Humic acid composition and application thereof

Abstract

A humic acid composition includes fulvic acid, ulmic acid and black humic acid. Fulvic acid is in an amount ranging from 64% to 98 wt % based on a sum weight of fulvic acid, ulmic acid and black humic acid. The content of phenol hydroxyl groups is in a range from 0.76 mmol/g to 2.42 mmol/g in the humic acid composition. The humic acid composition contains a high content of fulvic acid with small molecular weight and a high content of active functional groups, and a high content of phenolic hydroxyl in the active groups. The humic acid composition can provide microbial organic source for soil directly, and improve the nutrient of crops and the soil environment in rhizosphere to promote the breeding of microorganism and delay the attenuation rate of soil microbes.

Claims

1. A humic acid composition, comprising fulvic acid, ulmic acid and black humic acid, wherein, fulvic acid is in an amount ranging from 64% to 98 wt % based on a sum weight of fulvic acid, ulmic acid and black humic acid, and a content of phenol hydroxyl groups is in a range from 0.76 mmol/g to 2.42 mmol/g in the humic acid composition.

2. The humic acid composition according to claim 1, wherein the content of phenol hydroxyl groups is in a range from 1.12 mmol/g to 2.21 mmol/g in the humic acid composition.

3. The humic acid composition according to claim 1, wherein a total content of acidic groups is in a range from 0.76 mmol/g to 2.94 mmol/g in the humic acid composition.

4. The humic acid composition according to claim 2, wherein a total content of acidic groups is in a range from 0.76 mmol/g to 2.94 mmol/g in the humic acid composition.

5. The humic acid composition according to claim 3, wherein the total content of acidic groups is in a range from 1.12 mmol/g to 2.89 mmol/g.

6. The humic acid composition according to claim 4, wherein the total content of acidic groups is in a range from 1.12 mmol/g to 2.89 mmol/g.

7. The humic acid composition according to claim 1, wherein, a total humic acid is in an amount ranging from 25 parts to 65 parts by weight of the humic acid composition.

8. The humic acid composition according to claim 1, wherein, a total humic acid is in an amount ranging from 25 parts to 55 parts by weight of the humic acid composition.

9. The humic acid composition according to claim 1, wherein, organic material is in an amount ranging from 35 parts to 55 parts by weight of the humic acid composition.

10. The humic acid composition according to claim 7, wherein, water-soluble matter is in an amount ranging from 80 parts to 105 parts by weight of the humic acid composition.

11. The humic acid composition according to claim 9, wherein, water-soluble matter is in an amount ranging from 80 parts to 105 parts by weight of the humic acid composition.

12. The humic acid composition according to claim 1, wherein, sodium ions are in an amount ranging from 4.05 mmol/g to 4.85 mmol/g, or potassium ions are in an amount ranging from 8.53 mmol/g to 11.23 mmol/g in the humic acid composition.

13. The humic acid composition according to claim 2, wherein, sodium ions are in an amount ranging from 4.05 mmol/g to 4.85 mmol/g, or potassium ions are in an amount ranging from 8.53 mmol/g to 11.23 mmol/g in the humic acid composition.

14. The humic acid composition according to claim 3, wherein, sodium ions are in an amount ranging from 4.05 mmol/g to 4.85 mmol/g, or potassium ions are in an amount ranging from 8.53 mmol/g to 11.23 mmol/g in the humic acid composition.

15. The humic acid composition according to claim 1, wherein, sodium ions are in an amount ranging from 0.20 mmol/g to 0.31 mmol/g, potassium ions are in an amount ranging from 0.84 mmol/g to 1.43 mmol/g, and ammonium ions are in an amount ranging from 3.57 mmol/g to 8.07 mmol/g in the humic acid composition.

16. The humic acid composition according to claim 2, wherein, sodium ions are in an amount ranging from 0.20 mmol/g to 0.31 mmol/g, potassium ions are in an amount ranging from 0.84 mmol/g to 1.43 mmol/g, and ammonium ions are in an amount ranging from 3.57 mmol/g to 8.07 mmol/g in the humic acid composition.

17. The humic acid composition according to claim 3, wherein, sodium ions are in an amount ranging from 0.20 mmol/g to 0.31 mmol/g, potassium ions are in an amount ranging from 0.84 mmol/g to 1.43 mmol/g, and ammonium ions are in an amount ranging from 3.57 mmol/g to 8.07 mmol/g in the humic acid composition.

18. The humic acid composition according to claim 1, wherein, fulvic acid is in an amount ranging from 81% to 98 wt % based on a sum weight of fulvic acid, ulmic acid and black humic acid.

19. The humic acid composition according to claim 1, wherein, fulvic acid is in an amount ranging from 85% to 98 wt % based on a sum weight of fulvic acid, ulmic acid and black humic acid.

20. A humic acid fertilizer, soil amendment or plant growth stimulin comprising the humic acid composition according to claim 1.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is infrared spectrogram of the humic acid composition from wheat straw in embodiment 1.

(2) FIG. 2 is infrared spectrogram of the humic acid composition from rice straw in embodiment 2.

(3) FIG. 3 is infrared spectrogram of the humic acid composition from corn stalk in embodiment 3.

EMBODIMENTS

(4) Specific embodiments of the invention are further described in detail hereinafter, but not limiting the present invention.

Embodiment 1

(5) (1) crushing wheat straw to get a chopped straw with a length of 20-60 mm, washing the chopped straw for removing the mud, dehydrating the chopped straw to a content of the absolute dry straw material of more than 15%, and feeding the chopped straw into a screw feeder to compress and dehydrate continuously to a content of the absolute dry straw materials of 3545% to obtain plant straw material;

(6) (2) feeding the plant straw material in step (1) into a vertical continuous steamer, adding ammonium sulfite solution and mixing with the plant straw material, wherein, ammonium sulfite is an amount of 18% by weight of absolute dry straw material; adding an extract up to a liquid ratio of 1:7.5;

(7) (3) heating up to a temperature 175 C. in the vertical continuous steamer, and keeping the temperature for 120 min to obtain pulp liquid A;

(8) (4) blowing the pulp liquid A to a blow bin, passing a squeezing pulp machine and a vacuum pulp washer for washing and replacing to get a pulp and the extract;

(9) (5) obtaining a concentrated solution with a content of solid matter of 55% and a temperature of 100110 C. from the extract by multi-effect evaporator, and getting the humic acid composition by pulp-spraying granulation after stirring the concentrated solution;

(10) (6) applying the pulp in step (4) to paper making, or to getting polysaccharide by enzymolysis.

(11) Infrared spectrum of the humic acid composition in the embodiment is shown in FIG. 1. The humic acid composition in the embodiment is measured, in which a content of fulvic acid is 44.62 parts by weight, a content of ulmic acid is 4.6 parts by weight, a content of black humic acid is 0.81 parts by weight, and the total content of the humic acid is 50.03 parts by weight. The humic acid composition comprises a total of acidic groups of 2.12 mmol/g, carboxyl group of 0.31 mmol/g, phenolic hydroxyl group of 1.81 mmol/g, potassium ions of 1.29 mmol/g, ammonium ions of 8.07 mmol/g, and sodium ions of 0.31 mmol/g.

Embodiment 2

(12) (1) crushing rice straw to get a chopped straw with a length of 60 mm, washing the chopped straw for removing the mud, dehydrating the chopped straw to a content of the absolute dry straw material of more than 15%, and feeding the chopped straw into a screw feeder to compress and dehydrate continuously to a content of the absolute dry straw materials of 3545% to obtain plant straw material;

(13) (2) feeding the plant straw material in step (1) into a horizontal continuous steamer, adding potassium hydroxide and anthraquinone and mixing with the plant straw material, wherein, potassium hydroxide is an amount of 22% by weight of absolute dry straw material, and anthraquinone is an amount of 0.06% by weight of absolute dry straw material; adding water up to a liquid ratio of 1:3;

(14) (3) heating up to a temperature 155 C. in the horizontal continuous steamer, and keeping the temperature for 20 min to obtain pulp liquid A;

(15) (4) blowing the pulp liquid A to a blow bin, passing a squeezing pulp machine and a vacuum pulp washer for washing and replacing to get a pulp and the extract;

(16) (5) obtaining a concentrated solution with a content of the solid matter of 55% and a temperature of 100110 C. from the extract by multi-effect evaporator, and getting the humic acid composition by pulp-spraying granulation after stirring the concentrated solution;

(17) (6) applying the pulp in step (4) to paper making, or to getting polysaccharide by enzymolysis.

(18) Infrared spectrum of the humic acid composition in the embodiment is shown in FIG. 2. The humic acid composition in the embodiment is measured, in which a content of fulvic acid is 30.20 parts by weight, a content of ulmic acid is 1.5 parts by weight, a content of black humic acid is 5.97 parts by weight, and the total content of the humic acid is 38.67 parts by weight. The humic acid composition comprises total acidic groups of 0.95 mmol/g, carboxyl group of 0 mmol/g, phenolic hydroxyl group of 0.95 mmol/g, and potassium ions of 9.76 mmol/g.

Embodiment 3

(19) (1) crushing corn straw to get a chopped straw with a length of 20-100 mm, removing the dust from the chopped straw by dry method to obtain plant straw material;

(20) (2) feeding the plant straw material in step (1) into a ball steamer, adding sodium carbonate and anthraquinone and mixing with the plant straw material, wherein, sodium carbonate is an amount of 18% by weight of absolute dry straw material, and anthraquinone is an amount of 0.06% by weight of absolute dry straw material; adding an extract up to a liquid ratio of 1:3;

(21) (3) heating up to a temperature 160 C. in the ball steamer, and keeping the temperature for 120 min to obtain pulp liquid A;

(22) (4) blowing the pulp liquid A to a blow bin, passing a squeezing pulp machine and a vacuum pulp washer for washing and replacing to get a pulp and the extract;

(23) (5) obtaining a concentrated solution with a content of solid matter of 55% and a temperature of 100110 C. from the extract by multi-effect evaporator, and getting the humic acid composition by pulp-spraying granulation after stirring the concentrated solution;

(24) (6) applying the pulp in step (4) to paper making, or to getting polysaccharide by enzymolysis.

(25) Infrared spectrum of the humic acid composition in the embodiment is shown in FIG. 3. The humic acid composition in the embodiment is measured, in which a content of fulvic acid is 54.32 parts by weight, a content of ulmic acid is 0.74 parts by weight, a content of black humic acid is 0.54 parts by weight, and the total content of the humic acid is 55.6 parts by weight. The humic acid composition comprises total acidic groups of 2.17 mmol/g, carboxyl group of 0.19 mmol/g, phenolic hydroxyl group of 1.98 mmol/g, and sodium ions of 4.64 mmol/g.

Embodiment 4

(26) (1) stocking wheat straw to get a chopped straw with a length of 20-60 mm and a qualification rate of less than 92% by dry method, then wetly rubbing, washing the cropped straw, and dehydrating the chopped straw to a content of the absolute dry straw material of 18% to obtain plant straw material;

(27) (2) compressing and dehydrating the plant straw material to a content of the absolute dry straw materials of 40% to obtain plant straw material as dehygrated;

(28) (3) feeding the straw raw material obtained in step (2) to a vertical steamer; adding sodium carbonate solution and a black liquid with a temperature of 90 C., and adding a hot black liquid with a temperature of 150 C. into the vertical steamer, wherein, liquid ratio is 1:13, and sodium carbonate is an amount of 22% by weight of the absolute dry straw material; heating quickly and circularly a cooking liquor up to a temperature of 145 C. by a heater after the hot black liquid being fed into the vertical steamer, and keeping the temperature of 145 C. for 80 minutes under a pressure of 0.45 MPa at a top of the vertical steamer; discharging the hot black liquid and getting an intermediate pulp, wherein the hot black liquid as discharged enters into a tank for feeding circularly into the vertical steamer;

(29) (4) washing the intermediate pulp with a cold black liquid with a temperature of 75 C. to get a washed intermediate pulp; blowing the washed intermediate pulp to a squeezing pulp machine and a vacuum pulp washer for washing and replacing to get a dense pulp and a warm black liquid; after exchanging the heat of the warm black liquid, adding the black liquid and a cooking solution into the vertical steamer to proceed to step (3) circularly; and defibering the dense pulp to obtain a pulp.

(30) In a black liquid from the process of washing the intermediate pulp, a content of fulvic acid is 15 g/L, a content of ulmic acid is 10 g/L, a content of black humic acid is 5 g/L, the total content of humic acid is 30 g/L, a content of organic matter is 38 g/L, a content of the water-insoluble component is 16 g/L, a content of the solid matter is 80 g/L, and a content of sodium ions is 8.0 g/L. The humic acid composition of the present invention is got by evaporation and pulp-spraying granulation.

(31) The humic acid composition in the embodiment is measured, in which a content of fulvic acid is 15 parts by weight, a content of ulmic acid is 10 parts by weight, a content of black humic acid is 5 parts by weight, the total content of the humic acid is 30 parts by weight, and a content of organic matter is 38 parts by weight. The humic acid composition comprises total acidic groups of 1.00 mmol/g, carboxyl group of 0.1 mmol/g, and phenolic hydroxyl group of 0.9 mmol/g.

Embodiment 5

(32) (1) stocking wheat straw to get a chopped straw with a length of 20-60 mm and a qualification rate of less than 92% by dry method, then wetly rubbing, washing the cropped straw, and dehydrating the chopped straw to a content of the absolute dry straw material of 18% to obtain plant straw material;

(33) (2) compressing and dehydrating the plant straw material to a content of the absolute dry straw materials of 40% to obtain plant straw material as dehygrated;

(34) (3) feeding the straw raw material obtained in step (2) to a vertical steamer; adding sodium carbonate solution and a black liquid with a temperature of 90 C., and adding a hot black liquid with a temperature of 150 C. into the vertical steamer, wherein, liquid ratio is 1:10, and sodium carbonate is an amount of 15% by weight of the absolute dry straw material; heating quickly and circularly a cooking liquor up to a temperature of 140 C. by a heater after the hot black liquid being fed into the vertical steamer, and keeping the temperature of 145 C. for 80 minutes under a pressure of 0.45 MPa at a top of the vertical steamer; discharging the hot black liquid and getting an intermediate pulp, wherein the hot black liquid as discharged enters into a tank for feeding circularly into the vertical steamer;

(35) (4) washing the intermediate pulp with a cold black liquid with a temperature of 75 C. to get a washed intermediate pulp; blowing the washed intermediate pulp to a squeezing pulp machine and a vacuum pulp washer for washing and replacing to get a dense pulp and a warm black liquid; after exchanging the heat of the warm black liquid, adding the black liquid and a cooking solution into the vertical steamer to proceed to step (3) circularly; and defibering the dense pulp to obtain a pulp.

(36) In a black liquid from the process of washing the intermediate pulp, a content of fulvic acid is 35 g/L, a content of ulmic acid is 6 g/L, a content of black humic acid is 1 g/L, the total content of humic acid is 42 g/L, a content of organic matter is 45 g/L, a content of water insoluble matter is 20 g/L, a content of the solid matter is 90 g/L, and a content of sodium ions is 10.5 g/L. The humic acid composition of the present invention is got by evaporation and pulp-spraying granulation.

(37) The humic acid composition in the embodiment is measured, in which a content of fulvic acid is 35 parts by weight, a content of ulmic acid is 6 parts by weight, a content of black humic acid is 1 part by weight, the total content of the humic acid is 42 parts by weight, and a content of organic matter is 45 parts by weight. The humic acid composition comprises total acidic groups of 2.89 mmol/g, carboxyl group of 0.68 mmol/g, and phenolic hydroxyl group of 2.21 mmol/g.

Embodiment 6

(38) (1) wetting a treated bagasse by depithing in a mode of spray, and entering into a extrusion screw, to get bagasse material;

(39) (2) feeding the bagasse material in step (1) to a horizontal continuous steamer, adding sodium hydroxide and anthraquinone and mixing with the bagasse material, wherein, sodium hydroxide is an amount of 22% by weight of absolute dry straw material, and anthraquinone is an amount of 0.06% by weight of absolute dry straw material; adding water up to a liquid ratio of 1:3;

(40) (3) heating up to a temperature 155 C. in the horizontal continuous steamer, and keeping the temperature for 20 min to obtain pulp liquid A;

(41) (4) blowing the pulp liquid A to a blow bin, passing a squeezing pulp machine and a vacuum pulp washer for washing and replacing to get a pulp and the extract;

(42) (5) obtaining a concentrated solution with a content of the solid matter of 55% and a temperature of 100110 C. from the extract by multi-effect evaporator, and getting the humic acid composition by pulp-spraying granulation after stirring the concentrated solution;

(43) (6) applying the pulp in step (4) to paper making, or to getting polysaccharide by enzymolysis.

(44) The humic acid composition in the embodiment is measured, in which a content of fulvic acid is 38 parts by weight, a content of ulmic acid is 7 parts by weight, a content of black humic acid is 2 parts by weight, the total content of the humic acid is 47 parts by weight, and a content of organic matter is 45 parts by weight. The humic acid composition comprises total acidic groups of 2.89 mmol/g, carboxyl group of 0.68 mmol/g, phenolic hydroxyl group of 2.21 mmol/g.

Embodiment 7

(45) (1) wetting a treated bagasse by depithing in a mode of spray, and entering into a extrusion screw, to get bagasse material;

(46) (2) feeding the bagasse material in step (1) to a horizontal continuous steamer, adding potassium hydroxide and anthraquinone and mixing with the bagasse material, wherein, potassium hydroxide is an amount of 22% by weight of absolute dry straw material, and anthraquinone is an amount of 0.06% by weight of absolute dry straw material; adding water up to a liquid ratio of 1:3;

(47) (3) heating up to a temperature 155 C. in the horizontal continuous steamer, and keeping the temperature for 20 min to obtain pulp liquid A;

(48) (4) blowing the pulp liquid A to a blow bin, passing a squeezing pulp machine and a vacuum pulp washer for washing and replacing to get a pulp and the extract;

(49) (5) obtaining a concentrated solution with a content of the solid matter of 55% and a temperature of 100110 C. from the extract by multi-effect evaporator, wherein, the concentrated solution is the humic acid composition.

(50) The humic acid composition in the embodiment is measured, in which a content of fulvic acid is 43.1 g/L, a content of ulmic acid is 6.1 g/L, a content of black humic acid is 0.4 g/L, a content of the total acidic groups is 0.95 mmol/g, a content of carboxyl group is 0.02 mmol/g, and a content of phenolic hydroxyl group is 2.22 mmol/g.

(51) Further, the humic acid composition is obtained by pulp-spraying granulation after stirring the concentrated solution. In the humic acid composition, a content of fulvic acid is 43.1 parts by weight, a content of ulmic acid is 6.1 parts by weight, a content of black humic acid is 0.4 parts by weight, a total of acidic groups is 2.24 mmol/g, carboxyl group is 0.22 mmol/g, and phenolic hydroxyl group is 2.22 mmol/g.

(52) (6) applying the pulp in step (4) to paper making, or to getting polysaccharide by enzymolysis

Test Example 1 Experiment Method of Germination of Wheat Seed

(53) Soaking a certain quantity of wheat seeds with a certain concentration of humic acid solution for 4 hours in a culture dish, and adding an appropriate amount of water after filtering out the humic acid solution, and then culturing putting wheat seeds in an incubator under an appropriate temperature. Calculating the germination number every day, testing the germination potential on the third or fourth day, and testing the germination rate and the length of the seeding on the seventh day, and then getting the average value. The specific criterion and method for processing data are as follows. It is shown in formula (1-1), formula (1-2), formula (1-3).

(54) Standard of the Seed Germination:

(55) For long seed, the seed germination refers to a length of plumule being a half of length of seed

(56) For circular seed, the seed germination refers to a length of plumule being equivalent to a diameter of seed
Seeds germination rate: (total number of germination/total seed number)100% (measuring after 7 days)formula (1-1)
Germination potential: (germination number after n days/total seed number)100% (measuring after n days)formula (1-2)
Germination index: Gt/t Gt: the germination number in the t-th day
Activity index: Germination indexhypocotyl lengthformula (1-3)

(57) TABLE-US-00001 TABLE 1 The influence of the humic acid on the germination of wheat seed (50 grain/dish, T = 22 C., germination potential on the 4th day) Germi- Germination Germination nation Plumule Activity Samples potential (%) ratio (%) index length index Embodiment 1 91 90 28.94 7.68 221.55 Comparison 1 73 75 22.32 5.67 186.45 Embodiment 2 92 91 29.78 7.71 223.51 Comparison 2 74 76 22.45 5.69 187.13 Embodiment 3 93 92 29.91 7.85 224.64 Comparison 3 75 75 22.49 5.73 168.56

(58) Note: example 1, samples of embodiments 1-3 are the humic acid composition prepared by the method as embodiments 1-3 respectively. The comparison 1, 2 and 3 are the corresponding fulvic acid respectively. For example, in embodiment 1, the content of fulvic acid is 44.62 parts by weight in wheat straw, so, the sample of comparison 1 is fulvic acid with equivalent weight to the one of fulvic acid in the humic acid composition, and so on.

(59) It is seen from the above test results that, compared with fulvic acid with equivalent amount, the humic acid composition stimulates stronger to the germination of wheat seed. In other words, compared with fulvic acid with equivalent amount, the humic acid composition of the present invention also contains a little content of brown and black humic acid, phenolic hydroxyl with high content in the humic acid composition, except for fulvic acid. And the humic acid composition has obviously stimulating function on seed germination. The humic acid composition can provide microbial organic source for soil directly, and improve the nutrient of crops and the soil environment in rhizosphere to promote the breeding of microorganism and delay the attenuation rate of soil microbes. Thus, the soil microbes can be kept exuberant life activities for a long time, so as to promote the growth and development of crops.

Test Example 2

(60) 1. Material and Method

(61) 1.1 Test Method

(62) It is conducted a field experiment for two years of and a pot experiment for one year. The field experiment is conducted in an experimental field of Tai'an agriculture technology institution. The pot experiment is carried out in a net house. Experimental soil is taken from the same experimental field with the field experiment. And the experimental soil is aquic brown soil, and belongs to light soil. In the experimental soil, a content of organic matter content is 13.2 g/kg, a content of alkali solution nitrogen is 71.5 mg/kg, a content of rapidly-available phosphorus is 11.5 mg/kg, a content of rapidly-available potassium is 75.8 mg/kg, and pH 6.9.

(63) The humic acid composition for testing is prepared by the method in embodiment 1. A biological activity fertilizer with the humic acid is manufactured by Tai'an agriculture technology institution as a comparation, (including humic acid of 21.0%, NPK of 16.0%, beneficial microbes 2.010.07 cfu/g). And other inorganic fertilizer contains urea (including N 46.0%). Wheat varieties: Taishan 241.

(64) In experiment, it is provided basic application of the humic acid composition of embodiment 1 (sample) by 1500.0 kg/hm.sup.2, and basic application of the biological activity fertilizer (comparison) containing the humic acid by 1500.0 kg/hm.sup.2. For the field experiment, experimental plot is an area of 20.0 m.sup.2, and the experiment is replicated for 4 times by random assortment, there are an isolation area with a width of 1.0 m between replication blocks, and planting around for guarding row. For the pot experiment, a pot has a height of 27.0 cm and a diameter of 30.0 cm, the experiment is replicated for 6 times by random assortment, and displaying around protective pot to eliminate the edge growth effect.

(65) For the field experiment, sowing on Oct. 6, 2011, and harvesting on June, 3 in the next year; sowing seeds on Oct. 9, 20123, and harvesting on June 11 in the next year. For the pot experiment, sowing seeds on Oct. 10, 2012 and 2013, and harvesting on May 19 in the next year. In the field experiment, sowing wheat seeds with a weight of 15.0 g in each line after ditching, and basic seedlings is an amount of around 1.5 million/hm.sup.2. In the pot experiment, each pot is filled with a doped soil with a weight of 11.5 kg which is sieved by a screen with opening of 0.5 cm, sowing 15 seeds, and planting 10 wheat seedlings. Single basic application is conducted in the field experiment, and urea fertilizer of 150.0 kg/hm.sup.2 is applied in combination with watering at jointing stage in the next year. Soil is mixed with fertilizer in the pot experiment and soil mix, and urea fertilizer of 1.5 g/basin is applied in combination with watering at green period in the next year.

(66) 1.2 Researching the Determining Items

(67) After wheat maturation, for each experimental plot of the field experiment, it is independently performed to harvest, thresh, dry, remove impurity, and weigh, and then agronomy properties are researched on the representative plant. At the jointing stage and the postulation period for the pot experiment, the microorganism quantity and the enzymatic activity are determined in the soil sample. The total quantity of microorganism is determined by the plate isolation method in 10.sup.5 cfu/g (dry soil). The urease activity is determined by the phenol sodium chlorate colorimetric method in NH.sup.3N mg/(g.Math.d). The sucrose enzyme is determined by the 3,5-dinitrosalicylic acid colorimetric method in glucose mg/(g.Math.d). The phosphatase activity is determined by disodium phenyl phosphate method in phenol mg/(g.Math.d). The catalase activity is determined by potassium permanganate method in KMnO.sub.4 mL/(g.Math.d).

(68) 2. Results and Analysis

(69) 2.1 the Influence of Different Humic Acid on Wheat Growth and Yield

(70) 2.1.1 the Influence on Wheat Development

(71) Fixed-point investigation on the dynamic change of the wheat development in the field experiment during each fertilizing treatment, the results are shown in table 2.

(72) TABLE-US-00002 TABLE 2 the influence of different humic acid treatment on the dynamic variation of the wheat development (average value of the field experiments for two years) Basic Tiller number (10.sup.4/hm.sup.2) Percentage of seedlings Before Green Booting Maximum Effective earbearing Treatment (10.sup.4/hm.sup.2) winter stage stage tiller tiller tiller (%) Comparison 150.0 703.6 817.4 639.1 1545.1 471.0 30.5 Sample 150.0 671.8 814.6 666.7 1581.8 573.8 36.3

(73) The results show that the tiller number before winter and at green stage by applying the humic acid composition as sample is similar with the ones by applying the biological activity fertilizer as comparison. From the maximum tiller of wheat, the fertilizer efficiency of the humic acid composition of the present invention is gradually shown. The tiller number at the booting stage, the effective tiller quantity and the percentage of earbearing tiller are all higher than the ones by applying the biological activity fertilizer as comparison. It can be seen from the dynamic analysis of the wheat development, that the humic acid composition of the present invention has the properties with promoting antecedent growth and controlling post growth, so that it is provided the nutrition condition for obtaining a high yield of wheat.

(74) 2.1.2 the Influence on the Property and Yield of Wheat Plant

(75) The investigation results of difference treatment on the property and yield of wheat plant are shown in table 3

(76) TABLE-US-00003 TABLE 3 The influence on the property and yield of wheat plant by the different treatment Length Anti Flag Gains Plant of Internode fracture leaf Secondary Wheatear per height wheatear length (cm) forces area root number ear 1000-gains Treatment (cm) (cm) 1th 2th (g) (cm.sup.2) (No.) (10.sup.4/hm.sup.2) (No.) weight (g) Comparison 70.6 8.7 4.2 7.2 251.1 33.8 29.4 471.1 35.6 49.7 Sample 71.8 9.5 4.9 7.8 281.5 37.7 35.5 516.3 43.9 55.8

(77) It can be seen from the above results, compared with the biological activity fertilizer as comparison, there is an improvement with difference degrees in all of aspects such as length of wheatear, flag leaf area and secondary root, with the exception of plant height. Thus, the property of wheat plant meets more the requirements for high yield.

(78) 2.1.3 the Influence on Wheat Yield

(79) The results of the wheat yields with difference treatment are shown in table 4.

(80) TABLE-US-00004 TABLE 4 the influence on wheat yield by different treatment (average value of the field experiment for two years) Yield increase compared to the Yield in each plot Yield comparison Treatment I II III IV Average (kg/hm.sup.2) (kg/hm.sup.2) (%) Comparison 14.06 13.69 13.21 13.20 13.54 6774.0 Sample 17.73 16.97 16.97 16.96 17.16 7981.9 1107.9 17.8

(81) It can be seen from the above results, the yield by applying the humic acid composition of the present invention is higher than the one by applying the biological activity fertilizer as comparison by 17.8% under almost similar condition on applying the inorganic nutrients. The organic, inorganic nutrients and microbial in the humic acid composition of the present invention play a positive role in wheat yield.

(82) 2.2 the Influence on the Microbial Activity in Soil

(83) Soil microorganism and enzyme activity are the important index of soil fertility. The number of bacteria, fungi and actinomycetes are determined at the jointing stage and the postulation period for the pot experiment, the results are shown in table 5.

(84) TABLE-US-00005 TABLE 5 The influence on the quantity of soil microorganism by different treatments (pot experiment) Bacteria Fungus Actinomyces (10.sup.5 cfu/g, (10.sup.5 cfu/g, (10.sup.5 cfu/g, Test stage Treatment DW) DW) DW) Jointing Comparison 15.09 2.95 3.65 stage stage Sample 20.76 3.95 4.86 Postulation Comparison 11.72 1.63 2.77 period Sample 17.62 2.67 4.18

(85) It can be seen from the number attenuation of soil microorganism from the jointing stage to the postulation period that bacteria are attenuated by 22.3%, and funguses are attenuated by 44.7%, and actinomycetes are attenuated by 24.1% by applying the humic acid as comparison. Bacteria are attenuated by 15.1%, funguses are attenuated by 32.4%, and actinomycetes are attenuated by 14.0% by applying the humic acid composition of the present invention. It indicates that the humic acid composition can increase the quantity of soil microorganism significantly, and also keep the microorganism in high biological activity.

(86) 2.3 Influence of the Humic Acid on the Enzyme Activity in Soil

(87) The result of the enzyme activity in soil is shown at jointing stage of the pot experiment in table 6.

(88) TABLE-US-00006 TABLE 6 The influence of different fertilizing treatments on the enzyme activity in soil Urease Sucrase Phosphatase Catalase [NH.sup.3N glucose [phenol [KMnO.sub.4 Treatment mg/(g .Math. d)] mg/(g .Math. d)] mg/(g .Math. d)] mL/(g .Math. d)] Comparison 4.82 0.88 3.47 14.59 Sample 7.16 1.57 4.78 19.79
It can be seen from the above results, compared with the treatment with the humic acid as comparison, urease is increased by 2.34 mg/NH.sub.3N(g.Math.d), sucrase is increased by glucose 0.69 mg/(g.Math.d), phosphatase is increased by 1.31 phenol mg/(g.Math.d), and catalase is increased by 5.2 KMnO.sub.4 mL/(g.Math.d) by applying the humic acid composition of the present invention. It is indicated that the humic acid composition of the present invention is helpful to enhance the urease activity, and improve the efficiency of nitrogen element in soil. It is a result by providing more enzymatic substrate, such as enhancing the sucrase activity, speeding up the transformation of organic matter in soil, enhancing the activity of phosphatase in soil, enhancing the transformation and release of organic phosphorus in soil to improve the nutrition level of phosphor element in crop, and improving the activity of catalase, thus, the normal activity of soil microorganisms is effectively protected.

(89) The experiment is carried out on the humic acid compositions prepared in the other embodiments, the obtained results are similar with the foresaid.