Rumen protected glucose and method of preparation

Abstract

The present disclosure provides a preparation method of rumen protected glucose, comprising the following steps: (1) preparing glucose into a pellet and drying; (2) fluidizing the pellet in a fluidized bed after drying; and (3) coating melted aliphatic alcohol and/or saturated fatty acid onto the surface of the pellet located in the fluidized bed to obtain a rumen protected bypass glucose particle. The prepared rumen-protected glucose could go through the rumen of ruminant effectively; and the availability of coating layer in ruminant increases, making the coated glucose be released completely and fully utilized by ruminant, so ketosis or subclinical ketosis and fatty liver disease of the ruminants in perinatal stage could be prevented and reduced effectively, the postpartum weight loss could be reduced, and the cycle conception rate and the milk yield could be increased.

Claims

1. A method of preparing rumen protected glucose, comprising: mixing glucose and lipase, wherein the lipase is heat tolerant and comprises an enzyme activity of no less than 10,000 U/g, with the enzyme activity maintained no less than 85% at a temperature of 85 C., with a pH range of 3-11; rendering and drying the glucose/lipase mixture into glucose/lipase pellets; fluidizing the glucose/lipase pellets in a fluidized bed after drying; and coating all surfaces of the glucose/lipase pellets with melted aliphatic alcohols and/or saturated fatty acids in the fluidized bed.

2. A method of preparing rumen protected disaccharide and/or polysaccharide, comprising: mixing a disaccharide and/or a polysaccharide and lipase, wherein the lipase is heat tolerant and comprises an enzyme activity of no less than 10,000 U/g, with the enzyme activity maintained no less than 85% at a temperature of 85 C., with a pH range of 3-11; rendering and drying the disaccharide and/or polysaccharide/lipase mixture into disaccharide and/or polysaccharide/lipase pellets; fluidizing the disaccharide and/or polysaccharide/lipase pellets in a fluidized bed after drying; and coating all surfaces of the disaccharide and/or polysaccharide/lipase pellets with melted aliphatic alcohols and/or saturated fatty acids in the fluidized bed.

3. The method of preparing rumen protected glucose of claim 1, wherein the weight range of the glucose is 25 to 75%.

4. The method of preparing rumen protected glucose of claim 1 wherein the step of mixing the glucose and the lipase comprises mixing the lipase and the glucose at a ratio of 0.01 U-0.2 U of lipase per gram of glucose.

5. The method of preparing rumen protected glucose of claim 1, wherein the melting point of the saturated fatty acids is above 52 C., and wherein the saturated fatty acids comprise greater than 70% wgt of C.sub.16-C.sub.18 saturated fatty acids.

6. The method of preparing rumen protected disaccharide and/or polysaccharide of claim 2, wherein the weight range of the disaccharide and/or polysaccharide is 25%-75%.

7. The method of preparing rumen protected disaccharide and/or polysaccharide of claim 2, wherein the step of mixing disaccharide and/or polysaccharide with lipase comprises mixing the disaccharide and/or polysaccharide and the lipase at a ratio of 0.01 U-0.2 U of lipase per gram of disaccharide and/or polysaccharide.

8. The method of preparing rumen protected disaccharide and/or polysaccharide of claim 2, wherein the melting point of the saturated fatty acids is above 52 C., and wherein the saturated fatty acids comprise greater than 70% wgt of C.sub.16-C.sub.18 saturated fatty acids.

9. A method of preparing rumen protected glucose, comprising: mixing glucose and lipase, wherein the lipase is heat tolerant and comprises an enzyme activity of no less than 10,000 U/g, with the enzyme activity maintained no less than 85% at a temperature of 85 C., with a pH range of 3-11; rendering and drying the glucose/lipase mixture into glucose/lipase pellets; fluidizing the glucose/lipase pellets in a fluidized bed after drying; and coating all surfaces of the glucose/lipase pellets with melted aliphatic alcohols and/or saturated fatty acids in the fluidized bed to obtain a rumen protected glucose pellets, with a weight range of the glucose of 25%-75%.

Description

DETAILED DESCRIPTION OF THE EMBODIMENTS

(1) The present disclosure has been described using detailed descriptions of embodiments thereof that are provided by way of example and are not intended to limit the scope of the invention.

(2) The high temperature resistant lipase with an enzyme activity of 10000 U/g used in the following embodiments is provided by AsiaPac Bio-Technology Co., Ltd, the enzyme activity thereof could maintain above 85% at the temperature of 85 C. and maintain a high level at pH 3-11.

(3) The melting point of the saturated fatty acid used in the following embodiments is above 52 C., and the percentage by weight of C16C18 saturated fatty acid in the saturated fatty acid is 70%.

Example 1, 30% Rumen Protected Glucose was Prepared

(4) 30.5 kg of glucose was weighed and smashed to pass through 120 mesh sieve; meanwhile, 200 mg of high temperature resistant lipase with the enzyme activity of 10000 U/g was weighed and mixed into the glucose; then 8 kg of water was added to obtain the moist material; furthermore, the moist material was extruded with extruder and shot blasting was processed; and then drying was proceeded at a low temperature below 60 C. to obtain the glucose pellet.

(5) 65.5 kg of saturated fatty acid and 4 kg of chitosan were heated to 120 C. to obtain melts.

(6) The glucose pellet was fluidized in a fluidized bed of bottom spray coating machine, and the melts which were cooled to below 100 C. were sprayed onto the surface of the glucose pellet, then the rumen protected bypass glucose with 30% glucose was made.

Example 2, 50% Rumen Protected Glucose was Prepared

(7) 50.5 kg of glucose was weighed and smashed to pass through 120 mesh sieve; meanwhile, 400 mg of high temperature resistant lipase with the enzyme activity of 10000 U/g was weighed and mixed into the glucose; then 13 kg of water was added to obtain the moist material; furthermore, the moist material was extruded with extruder and shot blasting was processed; and then drying was proceeded at a low temperature below 60 C. to obtain the glucose pellet.

(8) 47.5 kg of saturated fatty acid and 2 kg of polyacrylic resin IV were heated to 120 C. to obtain melts.

(9) The glucose pellet was fluidized in a fluidized bed of bottom spray coating machine, and the melts which were cooled to below 100 C. were sprayed onto the surface of the glucose pellet, then the rumen protected bypass glucose with 50% glucose was made.

Comparative Example, 45% Rumen Protected Glucose was Prepared

(10) 45.5 g of glucose was weighed and smashed to pass through 120 mesh sieve; 51 g of saturated fatty acid and 4 g of nylon were weighed and heated to 160 C. to obtain melts; the glucose was added into the melts which were cooled to 100 C., the mixture was formed after even stirring, then the mixture was added into high pressure sprayer, sprayed into the fluidized bed with 20 C. cool air and cooled, then the rumen bypass glucose with 45% glucose was made.

Example 3 Example of Effects

(11) In order to testify the properties of the rumen bypass glucose, in vitro method was employed to simulate the digestive tract of the ruminant to proceed the stability test (the simulated rumen environment: buffer solution of pH 6.6 and 5.4, the simulated abomasum and duodenum environment: buffer solution of pH 2.4) and evaluation.

(12) Materials and Methods

(13) The buffer solutions of pH 6.6, 5.4 and 2.4 were employed in the study to simulate the ruminant's rumen, abomasum and duodenal gastrointestinal tract respectively.

(14) Formulation of Buffer Solutions of Different pH

(15) TABLE-US-00001 TABLE 1 Formulation of buffer solutions of different pH (g) Reagent pH 6.6 pH 5.4 pH 2.4 Citric acid 5.7225 9.297 19.698 (C.sub.6H.sub.8O.sub.7H.sub.2O) Sodium hydrogen 52.089 39.936 4.4392 phosphate (Na.sub.2HPO.sub.412H.sub.2O) (1) The substances listed in the table are dissolved in a small amount of distilled water, and adjusted to 1000 mL.
Test Samples

(16) Sample A: the rumen bypass glucose prepared in Example 1, the content of glucose: 30%;

(17) Sample B: the rumen bypass glucose prepared in Example 2, the content of glucose: 50%;

(18) Sample C: the rumen bypass glucose prepared in comparative example, the content of glucose: 45%;

(19) Three production batches for each sample, each 400 g, reserved;

(20) Stability Test for Rumen Protected Glucose at Buffer Solutions of Different pH

(21) 1.0000 g of samples A, B and C were accurately weighed and respectively added into the bottom of a 50 mL test tube with a stopper, 20 mL of buffer solution was respectively added in, then the test tubes were fastened with tube stoppers and digested in thermostatic shaking water bath of 39 C. for 2, 4, 8, 12 and 24 h. Samples A, B and C were washed after the test tubes were taken out and the buffer solutions were filtered, and the filter liquor was adjusted, then the content of the glucose in the filter liquor was measured. Based on the content of the glucose in the filter liquor, the rumen bypass percentage and the releasing percentage in small intestine of glucose were calculated. Each coated rumen bypass glucose sample was set three repetitions at each point in time.

(22) Test method of glucose: GB/T22221, a high performance liquid chromatography method of measuring the fructose, glucose, sugar, maltose and lactose in food.

(23) Computational Formula
Rumen bypass percentage of product(W1)=(1A2)/A1100%

(24) In Formulation: A1the content of glucose in product;

(25) A2the content of glucose in filter liquor of buffer solution of pH6.6.

(26) The releasing percentage of product in small intestine
(W2)=A3/A1100%

(27) In Formulation: A1the content of glucose in product;

(28) A3the content of glucose in filter liquor of buffer solution of pH2.4.
The effective releasing percentage of product=W1W2100%

(29) In Formulation: W1the rumen bypass percentage of product;

(30) W2the releasing percentage of product in small intestine.

(31) Statistical Approach

(32) SPSS 19.0 was used in statistical analysis.

(33) Result Analysis

(34) The Rumen Bypass Percentage of Each Product at Different Point-in-Time (pH6.6)

(35) It can be seen from Table 1 that, the rumen bypass percentage of each test sample is Sample A=Sample B>Sample C, wherein each test sample was cultivated in buffer solution of pH6.6 under the condition of 39 C. constant temperature bath.

(36) TABLE-US-00002 TABLE 1 The rumen bypass percentage of each product at different point-in-time(%) Time 2 h 4 h 8 h 12 h 24 h Sample A 96.40 93.51 80.50 73.00 69.50 Sample B 96.80 93.10 79.10 72.15 70.21 Sample C 82.00 72.00 64.90 58.20 50.20

(37) The Releasing Percentage of Each Product in Small Intestine at Different Point-in-Time (pH2.4)

(38) It can be seen from Table 2 that, the releasing percentage in small intestine of each test sample is Sample A=Sample B>Sample C, wherein each test sample was cultivated in buffer solution of pH2.4 under the condition of 39 C. constant temperature bath.

(39) TABLE-US-00003 TABLE 2 The release rate of product in small intestine of each product at different point-in-time(%) Time 2 h 4 h 8 h 12 h 24 h Sample A 92.20 97.30 100.00 100.00 100.00 Sample B 93.14 97.80 100.00 100.00 100.00 Sample C 89.00 92.00 94.50 95.30 97.40

(40) The Effective Releasing Percentage of Each Product at Different Point-in-Time

(41) It can be seen from Table 3 that, the effective releasing percentage of each test sample is Sample A=Sample B>Sample C, wherein each test sample was cultivated in buffer solution of pH6.6 and pH2.4 under the condition of 39 C. constant temperature bath.

(42) TABLE-US-00004 TABLE 3 The effective releasing percentage of each product at different point-in-time (%) Time 2 h 4 h 8 h 12 h 24 h Sample A 88.9 91.0 80.5 73.0 69.5 Sample B 90.2 91.1 79.1 72.2 70.2 Sample C 73.0 66.2 61.3 55.5 48.9

CONCLUSION

(43) Several rumen protected glucose products were cultured in vitro, and the rumen bypass percentage, the releasing percentage in small intestine and the effective releasing percentage of each product at different point-in-time were obtained by simulating ruminant rumen fluid and the small intestine fluid. The experiment results show the rumen bypass percentage, the releasing percentage in small intestine and the effective releasing percentage of the rumen bypass glucose of the products in the present disclosure have been significantly improved compared with those of product obtained in comparative example. The similar experiment effect could also be obtained when the weight percentage of C16C18 saturated fatty acid in the saturated fatty acid used in the preparation of samples A, B and C is more than 70%.

(44) The foregoing descriptions of specific embodiments of the present invention are presented for purposes of illustration and description. It is not intended to be exhaustive or to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and their practical application, to thereby enable others skilled in the art to make and utilize various exemplary embodiments of the present invention, as well as various alternatives and modifications thereof. The invention is to be limited only by the claims provided below and equivalents thereof.