ANTICANCER T CELL THERAPY PRODUCT-ASSISTING COMPOSITION COMPRISING DEPLETING ANTI-CD4 MONOCLONAL ANTIBODY AND USE THEREOF

Abstract

The present invention relates to an anticancer T cell therapy product-assisting composition comprising a depleting anti-CD4 monoclonal antibody and a use thereof. Accordingly, the composition comprising a depleting anti-CD4 monoclonal antibody according to the present invention is able to maximize the anticancer effect of a cancer antigen-specific anticancer T cell therapy product by maintaining an immunodeficient state and is thus effective. In addition, when administered twice or more times at regular intervals of 5 to 8 days, the composition exhibits a far superior effect.

Claims

1. A method of preventing or treating cancer, comprising: i) inducing transient immunodeficiency in a cancer patient; ii) administering cancer antigen-specific CD8 T cells and IL-2; and iii) inducing continuous immunodeficiency.

2. The method of claim 1, wherein the transient immunodeficiency is induced by irradiation or the administration of an anticancer agent.

3. The method of claim 2, wherein the anticancer agent is one or more selected from the group consisting of cyclophosphamide and fludarabine.

4. The method of claim 1, wherein the cancer antigen is any one or more autologous cancer antigens selected from the group consisting of human telomere reverse transcriptase (hTERT), Wilm's tumor antigen 1 (WT-1), NY-ESO-1, melanoma-associated antigen (MAGE), carcinoembryonic antigen (CEA), CA-125, MUC-1 and melanoma antigen recognized by T cells 1 (MART-1).

5. The method of claim 1, wherein the continuous immunodeficiency is induced by a depleting anti-CD4 monoclonal antibody.

6. The method of claim 5, wherein the depleting anti-CD4 monoclonal antibody is administered twice or more at intervals of approximately 5 to 8 days.

7. The method of claim 1, wherein the cancer in Step i) is any one selected from the group consisting of lung cancer, stomach cancer, breast cancer, colon cancer, liver cancer, prostate cancer, uterine cancer, brain cancer and sarcomas.

8. A method of maintaining immunodeficiency, comprising: i) inducing transient immunodeficiency in a cancer patient; and ii) administering a depleting anti-CD4 monoclonal antibody to the cancer patient in which the transient immunodeficiency is induced.

9. The method of claim 8, wherein the cancer of Step i) is any one selected from the group consisting of lung cancer, stomach cancer, breast cancer, colon cancer, liver cancer, prostate cancer, uterine cancer, brain cancer and sarcomas.

10. The method of claim 8, wherein the transient immunodeficiency is induced by irradiation or the administration of an anticancer agent.

11. The method of claim 8, wherein the depleting anti-CD4 monoclonal antibody is administered twice or more at intervals of approximately 5 to 8 days.

12. A composition for maintaining immunodeficiency, comprising a depleting anti-CD4 monoclonal antibody.

13. The composition of claim 12, wherein the composition is administered twice or more at intervals of approximately 5 to 8 days.

14. The composition of claim 12, wherein when the composition is treated, a period of maintaining immunodeficiency is approximately 10 days or more after the treatment of the composition.

15. A composition for helping an anticancer T cell therapy product, comprising a depleting anti-CD4 monoclonal antibody.

16. The composition of claim 15, which is administered twice or more at intervals of approximately 5 to 8 days.

17. The composition of claim 15, wherein the cancer is any one selected from the group consisting of lung cancer, stomach cancer, breast cancer, colon cancer, liver cancer, prostate cancer, uterine cancer, brain cancer and sarcomas.

18. A pharmaceutical composition used in prevention or treatment of cancer, comprising: a depleting anti-CD4 monoclonal antibody, cancer antigen-specific CD8 T cells, an immunodeficiency inducer and IL-2.

19. The pharmaceutical composition of claim 18, wherein the immunodeficiency inducer is one or more selected from the group consisting of cyclophosphamide and fludarabine.

20. The pharmaceutical composition of claim 18, wherein the cancer is any one selected from the group consisting of the lung cancer, stomach cancer, breast cancer, colon cancer, liver cancer, prostate cancer, uterine cancer, brain cancer and sarcomas.

Description

DESCRIPTION OF DRAWINGS

[0082] FIG. 1A shows that, when T cell- and B cell-deficient mice are treated with Pmel-1-specific CD8 T cells (aPmel-1) and additionally treated with whole body radiotherapy and IL-2 administration, the anticancer effect of aPmel-1 increases, resulting in a decreased size of cancer cells.

[0083] FIG. 1B shows mice in each group on days 18 and 90 after the drug treatment.

[0084] FIG. 2A shows the slowdown of the growth of cancer cells by an increase in dose of cyclophosphamide (CTX) and additional administration of aPmel-1 and IL-2.

[0085] FIG. 2B shows the increase in survival rate of mice by an increase in dose of CTX and additional administration of aPmel-1 and IL-2.

[0086] FIG. 2C shows the change in cell number of an inguinal lymph node according to a dose of CTX.

[0087] FIG. 2D shows the change in cell number of the spleen according to a dose of CTX.

[0088] FIG. 3A shows the process of maintaining an immunodeficient state for increasing the anticancer effect of aPmel-1 using a depleting anti-CD4 monoclonal antibody (dCD4).

[0089] FIG. 3B shows that the growth of cancer cells goes more slowly by increasing the anticancer effect of aPmel-1 when a depleting anti-CD4 monoclonal antibody is added during aPmel-1 treatment.

[0090] FIG. 3C shows that the survival rates of mice increase when a depleting anti-CD4 monoclonal antibody is added during aPmel-1 treatment.

[0091] FIG. 3D shows the change in proportion of CD8 cells administered to mouse CD45-positive cells to which a depleting anti-CD4 monoclonal antibody is additionally treated during aPmel-1 treatment.

[0092] FIG. 3E shows that the total cell number and CD8 cell number of a mouse additionally treated with a depleting anti-CD4 monoclonal antibody during aPmel-1 treatment being increased.

MODES OF THE INVENTION

Example 1

Confirmation of Anticancer Effect of Only CD8 T Cells

[0093] Excluding other immune cells with an anticancer effect, the anticancer effect of only cancer antigen-specific CD8 T cells was intended to be evaluated.

[0094] Specifically, 210.sup.5 B16-F10 melanoma cancer cells were subcutaneously injected into the dorsal area of a T cell or B cell-deficient RAG2.sup./ mouse to induce the formation of cancer tissue. At the same time, a cell suspension was prepared by collecting the lymph node and spleen of thymocyte antigen 1.1 (Thy1.1)+premelanosome protein-1 (Pmel-1) transgenic mice, and then CD8 T cells were isolated using anti-CD8 microbeads (Miltenyi Biotec). The isolated cells were suspended in a 10% fetal bovine serum (FBS)-containing RPMI1640 medium (Welgene) at a concentration of 210.sup.6 cells/mL, followed by dispensing into a culture dish. After adding 5 g/mL of hgp100 peptide (KVPRNQDWL, aa 25-33 of human gp100, Peptron), the cells were incubated for 2 days, thereby preparing activated Pmel-1 CD8 T cells (aPmel-1).

[0095] For an aPmel-1-administered group, aPmel-1 was washed with PBS twice, and administered into the RAG2.sup./ mice at a dose of 210.sup.6 cells/500 L/mouse through intravenous injection at 5 days after B16-F10 implantation. For a transient immunodeficiency-induced group, 6 hours before the administration of the Pmel-1 CD8 T cells, 6Gy irradiation was applied to the entire body to induce total body irradiation (TBI). For an IL-2-administered group, after aPmel-1 administration, 10,000 IU of recombinant human IL-2 was intraperitoneally injected once a day for 3 days.

[0096] As a result, as shown in FIG. 1A, after only 20 days, the cancer cell sizes in B16-F10 only-implanted mice (Control) exceeded 2000 mm.sup.3. In the mice into which only aPmel-1 was intravenously injected, it was shown that the growth of cancer tissue slowed down, and the mice survived for approximately 40 days. When IL-2 was administered in addition to aPmel-1, the cancer tissue grew more slowly, and some mice survived for up to 60 days. When transient immunodeficiency was induced by radiotherapy and then aPmel-1 was administered, some mice survived for up to 100 days, and even when IL-2 was administered additionally, a result similar to the previous result was shown.

[0097] In addition, as shown in FIG. 1B, when cancer tissue sizes of mice in each experimental group were analyzed at 18 days after the cancer cell administration, in the experimental group to which aPmel-1 and IL-2 were administered as well as irradiation, almost no cancer tissue was observed. Nevertheless, after approximately 60 days, the cancer tissue began to grow, and at approximately 90 days, the cancer tissue had considerably grown and thus could be visually observed. This shows that, although vitiligo, which is a type of autoimmune disease, was observed as a strong immune response against aPmel-1, B16-F10 cancer cells avoided this and grew again. Therefore, it was determined that B16-F10 cannot be completely removed only with administered aPmel-1.

Example 2

Confirmation of Anticancer Effect of CD8 T Cells and Determination of Dose of Added Transient Immunodeficiency Inducer

[0098] In Example 1, it had been confirmed that cancer cells cannot be completely removed only with aPmel-1, and thus to overcome this, it was expected that the activity of other immune cells such as T cells is needed. Therefore, in a normal immune system, it was intended to determine a dose of an added transient immunodeficiency inducer as well as confirmation of the anticancer effect of cancer antigen-specific CD8 T cells.

[0099] Specifically, 210.sup.5 B16-F10 melanoma cancer cells were subcutaneously injected into the dorsal area of a C57BL/6 mouse to induce the formation of cancer tissue. At the same time, aPmel-1 was prepared in the same manner as in Example 1. aPmel-1 and IL-2 were administered in the same manner as in Example 1, and transient immunodeficiency was induced by intraperitoneally injecting 150, 200 and 300 mg/kg of cyclophosphamide (CTX) two days before the aPmel-1 administration. In addition, to confirm a period of maintaining transient immunodeficiency according to the dose of CTX, 150, 200 and 300 mg/kg of CTX was intraperitoneally injected into C57BL/6 mice once, and an inguinal lymph node and the spleen of the mice were collected, followed by single cell suspension and then counting a cell number.

[0100] As a result, as shown in FIGS. 2A and 2B, in mice (NT) in which only B16-F10 was implanted and PBS was administered, a cancer cell size exceeded 2000 mm.sup.3 within only 20 days, and most of the mice died. In the 150 mg/kg CTX-administered mouse, cancer tissue grew more slowly, and the mouse survived for up to 35 days. Even when aPmel-1 and IL-2 were additionally administered, the growth of cancer tissue is similar to when only CTX was administered, and the maximum survival date was only 40 days. The 200 mg/kg CTX-administered mouse showed slower growth of cancer tissue than the 150 mg/kg CTX-administered mouse, and survived for up to 45 days. When aPmel-1 and IL-2 were additionally administered, cancer tissue grew more slowly than when only CTX was administered, but had a similar size after 40 days and survived for up to 50 days. The 300 mg/kg CTX-administered mouse showed slower growth of cancer tissue than when 200 mg/kg CTX-administered mouse, and survived for up to 60 days. When aPmel-1 and IL-2 were additionally administered, it was confirmed that cancer tissue grew more slowly than when only CTX was administered, and even after 60 days had a size of only approximately 600 mm.sup.3, and the mouse has a survival rate of 40%.

[0101] As the dose of the added CTX increases, the period of maintaining a transient immunodeficiency effect increases, confirming that the anticancer effect caused by aPmel-1 increases. However, when 300 mg/kg or more of CTX was administered, it can be toxic to the mouse, leading to the death of the mouse, and therefore it is not suitable for induction of transient immunodeficiency, and when 150 mg/kg CTX was administered, a transient immunodeficiency effect was insignificant. As shown in FIGS. 2C and 2D, even when 200 mg/kg CTX was administered, since it was confirmed that the immunodeficiency effect was maintained for approximately 2 weeks, the dose of the added CTX was 200 mg/kg. However, it was confirmed that this short period achieved a sufficient anticancer effect from aPmel-1.

Example 3

Effect of CD4 Depletion on Anticancer Effect of Pmel-1 CD8 T Cells

[0102] When partial immunodeficiency was continuously induced using a depleting anti-CD4 monoclonal antibody, it was intended to confirm whether it affects the anticancer effect of cancer antigen-specific CD8 T cells. It was also intended to confirm whether the number of Thy1.1.sup.+Pmel-1 CD8.sup.+ T cells and the inherent number of CD8 T cells of a cancer patient were changed.

[0103] Specifically, experimental groups were prepared in the same manner as in Example 2. When continuous immunodeficiency was induced, from 10 days after B16-F10 implantation, 200 g of GK1.5 (depleting anti-CD4 monoclonal antibody) was intraperitoneally administered five times at intervals of 8 days (FIG. 3A). To confirm the change in CD8 T cell number, at 10, 17, 24, 31 and 38 days after the cancer cell administration, blood was collected from mice of each experimental group, stained with anti-CD45, anti-CD8 and anti-Thy1.1 antibodies, and then a proportion of Thy1.1.sup.+CD8.sup.+ cells was analyzed from CD45-positive cells through flow cytometry. Particularly, at 20 days after the cancer cell administration, the proportion and number of CD8 T cells in each organ were analyzed.

[0104] As a result, as shown in FIGS. 3B and 3C, mice (PBS) in which only B16-F10 was implanted and PBS was administered had a cancer cell size of more than 2000 mm.sup.3 within only 20 days, and most of the mice died. The mice to which aPmel-1 CD8 T cells and IL-2 were administered after 200 mg/kg CTX was administered began to show the rapid growth of cancer cells from 25 days, and survived for up to approximately 50 days. When the depleting anti-CD4 monoclonal antibody was administered under the same conditions as described above, the cancer cells grew insignificantly from day 35, but compared with that before the depleting anti-CD4 monoclonal antibody was administered, the growth of the cancer cells was slowed a maximum of 8-fold or more, and thus even after 60 days, the size was less than 500 mm.sup.3. In addition, 60% of the mice survived for 60 days or more. Even compared with the case in which 300 mg/kg CTX was administered and the depleting anti-CD4 monoclonal antibody was not administered (FIG. 2B), the size of a cancer cell was reduced 100 mm.sup.3 or more, and the proportion of the mice surviving for 60 days or more also increased 20% or more.

[0105] In addition, as shown in FIG. 3D, when the depleting anti-CD4 monoclonal antibody was continuously administered, compared to the case without administration, the proportion of the Thy1.1.sup.+CD8.sup.+ T cells was maintained at a higher level. Additionally, after the administration of the depleting anti-CD4 monoclonal antibody was stopped, the proportion of the Thy1.1.sup.+CD8.sup.+ T cells was gradually reduced after 4 to 5 days.

[0106] Particularly, as shown in FIG. 3E, compared with when CTX and aPmel-1 were administered, when a depleting anti-CD4 monoclonal antibody (dCD4) was additionally administered to the inguinal lymph node (TDLN), the total cell number increased. Among these, the number of CD8 T cells increased approximately 2.5 fold, compared with the case in which only CTX and aPmel-1 were administered, and particularly, it was confirmed that the CD8 T cells (Thy1.1.sup.CD8.sup.+ T cells, black bar) in C57BL/6 mouse, compared with the administered Thy1.1.sup.+CD8.sup.+ T cells (gray bar), greatly increases. The proportion of CD8 T cells in the spleen also increased similar to the result of the inguinal lymph node.

[0107] In the case of CD8 T cells (TIL) invading cancer tissue, when only CTX and aPmel-1 were administered, the proportion of Thy1.1.sup.+CD8.sup.+ T cells was highly exhibited, but when a depleting anti-CD4 monoclonal antibody was additionally administered, the proportion of Thy1.1.sup.CD8.sup.+ T cells was higher.

[0108] As a result, when partial immunodeficiency was continuously maintained through anti-CD4 monoclonal antibody depletion (anti-CD4 depletion), since in vivo proliferation of the administered CD8 T cells may be promoted, and the proliferation of CD8 T cells, which is inherent in a cancer patient, is induced, it is determined that the anticancer effect of the T cell therapy product, that is, aPmel-1, may increase.

INDUSTRIAL APPLICABILITY

[0109] The immunodeficiency-maintaining effect using a depleting anti-CD4 monoclonal antibody provided in the present invention can allow the effects of various anticancer therapies such as a T cell therapy product to be sufficiently exhibited in various cancer patients and thus can be effectively used in industries associated with prevention or treatment of cancer, indicating high industrial availability.