METHOD FOR PRODUCING A HIGH-PRESSURE TREATED PLANT SEED BASE PRODUCT, AND PLANT SEED BASE PRODUCT
20230094378 · 2023-03-30
Assignee
Inventors
- Mike RICHTER (Darmstadt, DE)
- Astrid SCHÖNBERGER (Darmstadt, DE)
- Daniel BONERZ (Bensheim, DE)
- Julian ASCHOFF (Griesheim, DE)
Cpc classification
A23C11/10
HUMAN NECESSITIES
A23V2002/00
HUMAN NECESSITIES
International classification
Abstract
A method provides a base for milk substitute products from plant seeds which gives a smooth mouthfeel similar to that of the corresponding product from animal milk. The method comprises the steps of: a) soaking plant seed feedstock in water, and b) high-pressure homogenizing of the liquefied plant seed feedstock at a pressure of at least 800 bar, preferably at least 1000 bar, most preferably at least 2000 bar.
Claims
1.-16. (canceled)
17. A method for producing a plant seed base product, without removing any components of the seeds used, comprising the steps of: a) soaking plant seed feedstock in water, the plant seed feedstock comprising at least seeds selected from the group consisting of cereals, pseudocereals, other plant seeds, and mixtures of these seeds; b1) liquefying the mixture from step a) under the action of enzymes to produce a liquefied plant seed feedstock; and b) high-pressure homogenizing the liquefied plant seed feedstock at a pressure of at least 800 bar.
18. The method of claim 17, wherein step a) is preceded by a step a1) of adding at least one enzyme.
19. The method of claim 18, wherein the at least one enzyme comprises one or more of amylase, lipase, β-glucanase, protease, and cellulase.
20. The method as claimed in claim 17, wherein at least one of the enzymes has a hydrolytic activity of at least 5% towards dietary fibers.
21. The method as claimed in claim 17, wherein step b) is preceded by a step b2) of deactivating at least one enzyme.
22. The method of claim 21, wherein the deactivating of at least one enzyme is achieved by heating and by altering a pH value.
23. The method of claim 21, wherein the deactivating is performed at temperatures in a range between 120° C. and 150° C. over a duration of up to 5 minutes.
24. The method of claim 21, wherein the deactivating is performed at temperatures at a maximum temperature of 95° C. over a duration of up to one hour.
25. The method as claimed in claim 22, wherein prior to the heating, the pH value is adjusted in the range from 3 to 5.
26. The method as claimed in claim 22, wherein following the heating the pH value is adjusted in the range from 6 to 8.
27. The method as claimed in claim 17, wherein step b) is preceded by a step b11) of comminuting the plant seed feedstock; and/or by a step b111) of comminuting the liquefied plant seed feedstock.
28. The method as claimed in claim 17, wherein step b) is followed by a step c) of keeping hot the high-pressure treated plant seed base product at a temperature in the range from 65° C. to 95° C.
29. The method as claimed in claim 17, wherein step b) comprises high-pressure homogenization of the liquefied plant seed feedstock at a pressure of at least 2000 bar.
30. A plant seed base product produced by the method according to claim 17, wherein the plant seed base product comprises all components of the at least one used plant seed or their degradation products, respectively, and wherein a volume density distribution of particles of the plant seed base product in which d3.97 is not more than 130 micrometers.
31. The plant seed base product of claim 30, wherein a percentage of plant seeds in the plant seed base product is up to 50 wt. %.
32. The plant seed base product as claimed in claim 30, wherein the plant seed base product comprises at least one seed selected from the group consisting of cereals, pseudocereals, oilseeds and legume seeds, and mixtures of these seeds.
33. A foodstuff or an additive to a foodstuff comprising the plant seed base product as claimed in claim 30, wherein the foodstuff is selected from the group consisting of alternatives to milk and milk products, beverages, drinking milk, milkshakes, drinking yoghurt, yoghurt, and ice cream preparations.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0051] The invention will now be explained in more detail by way of exemplary embodiments and with reference to the accompanying figures, wherein:
[0052]
[0053]
[0054]
[0055]
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[0059]
[0060]
DETAILED DESCRIPTION
[0061]
[0062] In the accompanying figures, the stated method steps according to the basic scheme are framed by thicker lines than the method steps according to the refinements.
[0063] According to the basic scheme of the method according to the invention as shown in
[0064] In the illustrated exemplary embodiment, the mash has a dry mass content of 17.6 wt. % and is heated to a temperature of 50° C.
[0065] After heating, soaking of the plant seed feedstock in water begins. The plant seed feedstock will swell for a holding time as selected by a person skilled in the art, and will thereby absorb water.
[0066] In the exemplary embodiment as illustrated in
[0067] The method step referred to as “liquefying” converts the mash into a homogeneous, flowable, in particular pumpable fluid. What is provided is a suspension of water-insoluble plant seed components in an aqueous phase which in particular contains proteins, starch, and sugars. These components are included in dissolved form, at least in part.
[0068] In order to ensure that the native starch molecules have been adequately degraded to sufficiently reduce the viscosity of the liquefied plant seed feedstock prior to the high-pressure homogenization, an iodine solution starch test can be performed on a sample of the product prior to entering the high-pressure homogenization.
[0069] The liquefied plant seed feedstock has a specific gravity of 17° Brix. It is conveyed through a nozzle, using at least one high-pressure pump, whereby the liquefied plant seed feedstock according to the invention is subjected to a significantly higher pressure load in comparison to conventional high-pressure homogenizers. Therefore, within the scope of the inventive method, the high-pressure homogenization is also referred to as “ultra-high-pressure homogenization” (UHPH for short). In the illustrated exemplary embodiment, the pressure is 2000 bar. The high-pressure homogenization produces the plant seed base product according to the invention from the liquefied plant seed feedstock. According to the exemplary embodiment illustrated in
[0070] For example, in order to adjust the flow behavior of the plant seed feedstock and of the liquefied plant seed feedstock, a further embodiment of the invention offers the possibility of influencing the composition of the plant seed feedstock in an enzymatic way.
[0071] The pH is adjusted by adding acid, for example hydrochloric acid HCl, to obtain a pH value in a target range between 6.2 and 6.4. Subsequently, at least one enzyme is added. In the illustrated exemplary embodiment, a β-glucanase was used in a concentration of about 0.5 kg/MT of plant seed feedstock. This concentration has proven to be suitable when using oat flour. Furthermore, an alpha-amylase was added in the illustrated exemplary embodiment. For this enzyme, a concentration of about 1.0 kg/MT plant seed feedstock has proven to be suitable when using oat flour. The unit “MT” means “metric ton” (1000 kg), the specified values indicate the amount of enzyme used in kg per 1000 kg of seed feedstock.
[0072] In order to determine the hydrolytic activity of the employed enzyme, an oat flour was diluted with water, the enzyme was added and the mixture was wet-milled. The oat flour had a residual moisture content of 5%. Mixing it with water creates the “starting product” for the rest of the process, which has a moisture content of 62.79%.
[0073] In each case, the long-chain and short-chain dietary fiber fractions were determined. The sum of these fractions gives the total content of dietary fibers. For better comparability, these values were only related to the dry mass (residual moisture content of 0%), and a difference between the flour and the product was calculated for the individual fractions.
[0074] In the table given below, the abbreviation “HMWDF” stands for “high molecular weight dietary fiber” and “LMWDF” stands for “low molecular weight dietary fiber”.
[0075] The percentages or fractions of dietary fibers are given in g/100 g.
TABLE-US-00001 Starting Starting Differ- Oat flour product Oat flour product ence Residual 5% 62.79% 0% 0% moisture Dietary 13.37 ± 1.97 4.68 ± 0.96 14.07 ± 2.07 12.58 ± 2.58 −11% fibers HMWDF 12.14 ± 1.85 3.24 ± 0.74 12.78 ± 1.95 8.71 ± 1.99 −32% content thereof LMWDF 1.23 ± 0.38 1.44 ± 0.42 1.29 ± 0.40 3.87 ± 1.13 200% content thereof
[0076] It is clearly apparent that the dietary fibers are affected. On the one hand, the total content of dietary fibers in the examined oat flour example drops by 11% compared to the raw material. Furthermore, a clear shift can be seen from the fraction of long-chain dietary fibers (HMWDF) to the fraction of smaller dietary fibers (LMWDF). Hence, the native dietary fibers are affected or attacked by the hydrolytic activity of the enzymes.
[0077] The results suggest that very large β-glucan molecules are broken down into medium-sized pieces.
[0078] Within the context of the invention, independently of the exemplary embodiment explained above, and similarly to the β-glucans found in oats and barley, the advantage of the hydrolytic breakdown of dietary fibers also applies to other soluble dietary fibers that increase the viscosity of a mixture made up of seed feedstock and water, such as to pentosans in rye or mucilage in linseed.
[0079] By breaking down these dietary fibers, the viscosity of the mixture of seed feedstock and water is greatly reduced, thereby allowing for a higher mixing ratio of seed feedstock to water, e.g. flour to water, by virtue of the invention. At the same time, the effort involved in pumping the mass is reduced, and better processability is generally facilitated.
[0080] Furthermore, water-soluble dietary fibers such as β-glucans are often associated with a “slimy” mouthfeel, hence their breakdown can improve the sensory impression.
[0081]
[0082] Then, the acidified liquefied plant seed feedstock is heated to a temperature of 95° C. and maintained at this temperature for a duration of 5 minutes. Optionally, the acidified plant seed feedstock can be cooled off to a temperature of 20° C. after the holding time, for example in order to reduce the stress on components of the employed apparatus, such as seals.
[0083] Prior to high-pressure homogenization, the pH value is neutralized by adding a lye, for example sodium hydroxide solution NaOH, to obtain a pH value in the target range of 6.7 to 7.0.
[0084]
[0085]
[0086] Thus, a comminution step can optionally be performed after the soaking. For example, such comminution can be achieved using a rotor-stator dispersing device, in particular a cutting mill. A “Turrax” used inline has proven to be particularly useful in a simple manner. Within the scope of the invention, comminuting is also possible through a high-pressure treatment which is performed at significantly lower pressures than the actual high-pressure homogenization, for example at pressures of up to 300 bar.
[0087] The high-pressure homogenization can be preceded by a comminution process as described above, which can be carried out in addition to or as an alternative to the optional comminution described above. Especially when using flakes or whole grains as the plant seed feedstock, the optional comminution will be helpful in the inventive method in order to be able to adapt the flowability of the (liquefied) plant seed feedstock to the respective requirements of the process.
[0088]
[0089]
[0090] In addition to the embodiments shown in
Exemplary Embodiment 1
[0091] A mixture made up of oat flour and water was used, with a dry mass content of 15 wt. %. Oat flour usually has a residual moisture content of not more than 12 wt. %. The flour used in this example had a residual moisture content of approx. 9 wt %. Prior to the ultra-high pressure treatment, comminution was carried out using a Turrax (IKA® Process-Pilot 2000/04) operated in-line at 12800 rpm at a back pressure of 1 bar. Samples ultra-high pressure-treated at treatment pressures of 1000, 2000, 3000, and 4000 bar were examined.
[0092] It was found that for this dry mass content, the best results were achieved at values between 2000 and 3000 bar.
[0093] The photographs in
[0094] Tastings of the samples revealed that at pressures above 1000 bar a structure with a smooth mouthfeel was produced. In the case of the samples produced at 2000 bar, some roughness was noticeable on the tongue, and the samples produced at 3000 bar did not show this roughness, but had a thinner texture than the samples produced at 2000 bar.
[0095] After a stability test by centrifugation at 4000 g for 10 minutes, the samples prepared at 2000 bar and 3000 bar showed less phase separation than the samples prepared at 1000 bar and 4000 bar.
[0096] The following table summarizes the results of particle size analysis (Malvern Panalytical, Mastersizer 3000; refractive index disperse phase: 1.449; refractive index dispersant: 1.330; light shading: 10-15%), which were performed on the materials that had been treated at the specified pressures. Here, the light shading value is a measure for the dilution, which cannot be converted into SI units. However, this specification will be sufficient for a person skilled in the art to comprehend and trace the measurement with this device and software. Given below are the parameters d.sub.3.97, d.sub.3.50, and d.sub.3.10, in micrometers, of the volume density distribution of the particles.
TABLE-US-00002 Parameter d.sub.3.97 d.sub.3.50 d.sub.3.10 1000 bar 176 32 0.894 2000 bar 118 28 1.12 3000 bar 88.7 24.2 1.76 4000 bar 82.9 19.6 1.86
[0097] The following results of a viscosity measurement (Anton Paar rheometer MCR 102; measuring body: ST-24; temperature=10° C.) confirm the findings, according to which the range of 2000 bar found for the stated dry mass content of the wholegrain oat material is preferred over the other pressures examined.
TABLE-US-00003 Viscosity in mPa .Math. s @ shear rate of 5/s 11.4/s 21/s 49.8/s 101/s 1000 bar 242.1 172.4 91.7 59.3 49.8 2000 bar 441.6 311.4 163.5 101.6 77.8 3000 bar 156.7 123.7 78.5 57.7 50.8 4000 bar 138.1 106.5 67.4 49.1 43.3
[0098] A variation of the product described above with a dry mass content of 20 wt. % also resulted in stable products when prepared by an ultra-high pressure treatment at 2000 bar and at 3000 bar.
[0099] Furthermore, it was found that lactic acid fermentation of the wholegrain oat base materials according to the invention is possible and led to yoghurt-like products with a sour taste classified as pleasant from a sensory point of view. For products with a texture similar to a milk-based yoghurt, dry mass contents above 20 wt. % should be aimed for when using wholegrain oat flour.
Exemplary Embodiment 2
[0100] A mixture made up of oat flour and water with an oat content of 35 wt. % was used. Oat flour usually has a residual moisture content of not more than 12 wt. %. The flour used in this example had a residual moisture content of approx. 9 wt %.
[0101] Prior to the ultra-high pressure treatment, comminution was carried out using a Turrax (IKA® Process-Pilot 2000/04) operated in-line at 12800 rpm at a back pressure of 1 bar, or using a high-pressure homogenizer at 300 bar. No significant differences were found on the product between these two methods of comminution.
[0102] Samples ultra-high pressure-treated at treatment pressures of 2000, 2500, and 3000 bar were examined.
[0103] The following table summarizes the results of particle size analysis (Malvern Panalytical, Mastersizer 3000; refractive index disperse phase: 1.449; refractive index dispersant: 1.330; light shading: 10-15%), which were performed on the materials that had been treated at the specified pressures. Given below are the parameters d.sub.3.97, d.sub.3.50, and d.sub.3.10, in micrometers, of the volume density distribution of the particles.
TABLE-US-00004 Parameter d.sub.3.97 d.sub.3.50 d.sub.3.10 2000 bar 133 23.5 1.01 2500 bar 113 22.6 0.899 3000 bar 97.3 22.5 0.952
[0104] High-pressure homogenization within the scope of the invention, at a pressure above 2000 bar, in particular at 2500 bar or 3000 bar, allows to produce wholegrain oat base products with a dry mass content of 35 wt. % and a smooth texture in the mouthfeel. In terms of taste, the sample produced at 3000 bar was preferred in the sensory evaluation. The highest viscosity and the best mouthfeel among the samples examined was achieved with a pressure of 2500 bar.
[0105] The stability of a “ready to drink” product is better with a pressure of 3000 bar than that of a corresponding product that has undergone an ultra-high pressure treatment at 2000 bar. Here, better stability means a lower proportion of supernatant forming in the sample over a storage time of up to 72 hours, for example.
[0106] In this example, the samples were subjected to a subsequent sterilization process step at 141° C. for a duration of 4 s and were treated downstream in a two-stage high-pressure homogenizer at 250 bar in the first stage and 50 bar in the second stage. It was found that this subsequent process step narrows the particle size distribution by shifting the d.sub.3.10 parameter towards larger and the d.sub.3.97 parameter towards smaller values.
Exemplary Embodiment 3
[0107] The same mixture made up of oat flour and water as in exemplary embodiment 2 was used and the effect of repeated multiple homogenization was examined.
[0108] The following table summarizes the results of particle size analysis of these studies. The measurements were performed using the “Mastersizer 3000” from Malvern Panalytical, with the following parameters: refractive index disperse phase: 1.449; refractive index dispersant: 1.330; light shading: 10-15%. Given below are the parameters d.sub.3.97, d.sub.3.50, and d.sub.3.10, in micrometers, of the volume density distribution of the particles.
TABLE-US-00005 Parameter d.sub.3.97 d.sub.3.50 d.sub.3.10 Number of passes @ 300/50 bar 1 423 52.1 3.25 2 318 48.3 3.1 3 255 47.6 3.07 4 223 41.6 2.92 5 222 44.7 2.90 10 175 38.0 2.62 15 154 35.7 2.58 17 159 35.0 2.69
[0109] What was found is that it is impossible with multiple passes through a two-stage high-pressure homogenizer of the APV Gaulin LAB 60/500/2 type at 300/50 bar, to achieve a similar result as with the method according to the invention. The specification “300/50 bar” means that a total pressure of 300 bar is built up. The pressure drops from 300 bar to 50 bar via a first valve. This 50 bar is relieved to ambient pressure via a second valve. In fact, multiple repetition of homogenization at 300/50 bar permits to approximately obtain the particle size of a treatment according to the invention at 1000 bar. The maximum particle size is somewhat higher than with an ultra-high pressure treatment according to the invention at 1000 bar, but the d.sub.3.97 diameter is significantly higher. Adequate comminution for a pleasantly smooth mouthfeel and a particle size of less than 130 micrometers is only achieved at the higher pressures according to the invention.
[0110] Even repeated homogenization at 1000 bar does not achieve the same result as a high-pressure treatment according to the invention at 3000 bar.
TABLE-US-00006 Parameter d.sub.3.97 d.sub.3.50 d.sub.3.10 Number of passes @ 1000 bar 1 199 32.2 1.77 2 218 29.9 1.53 3 162 26.7 1.33 4 136 25.1 1.22 5 126 23.9 1.17 10 134 24.7 1.22
Exemplary Embodiment 4
[0111] A mixture made up of wholegrain rice flour and water with a wholegrain rice content of 35 wt. % was used. Wholegrain rice flour has a maximum residual moisture content of 14.5 wt. %. The wholegrain rice flour used in conjunction with this embodiment had a residual moisture content of about 12 wt. %. In comparison to the results obtained with wholegrain oats (exemplary embodiment 2) it was found that the processing of wholegrain rice is similarly possible.
[0112] Prior to the ultra-high pressure treatment, comminution was carried out using a “Turrax” (IKA® Process-Pilot 2000/04) operated in-line at 12800 rpm with a back pressure of 1 bar, or using a high-pressure homogenizer at 300 bar. No significant differences were found on the product between these two methods of comminution.
[0113] Samples ultra-high pressure treated at treatment pressures of 1000, 2000, 3000, and 4000 bar were examined.
[0114] The following table summarizes the results of particle size analysis (Malvern Panalytical, Mastersizer 3000; refractive index disperse phase: 1.449; refractive index dispersant: 1.330; light shading: 10-15%), which were performed on the materials that had been treated at the specified pressures. Given below are the parameters d.sub.3.97, d.sub.3.50, and d.sub.3.10, in micrometers, of the volume density distribution of the particles.
TABLE-US-00007 Parameter d.sub.3.97 d.sub.3.50 d.sub.3.10 1000 bar 134 17.0 3.40 2000 bar 87.1 13.5 2.72 3000 bar 66.4 11.9 2.19 4000 bar 48.9 11.2 3.85
[0115] Within the scope of the invention, high-pressure homogenization at a pressure above 2000 bar, in particular at 2500 bar or 3000 bar, allows to prepare wholegrain rice base products with a smooth texture in the mouth. The samples produced under at least 3000 bar exhibited better stability than the other samples. Here, better stability means a lower proportion of supernatant forming in the sample over a storage period.
Exemplary Embodiment 5
[0116] A mixture made up of 6.65 wt. % of golden linseed flour and water was used. Golden linseed flour has a maximum residual moisture content of 10 wt. %. The golden linseed flour used in this embodiment had a residual moisture content of about 9 wt. %.
[0117] In comparison to the results obtained with wholegrain oats and wholegrain rice (exemplary embodiments 2 and 3) it was found that the processing of golden linseed flour, i.e. a flour from an oilseed, is likewise possible within the scope of the invention.
[0118] The golden linseed flour that was used is flour from press cake after oil extraction, which is finely ground, and which therefore has a lower fat content than the whole seed. The proportion of linseed used was reduced in comparison to the other flours according to the above exemplary embodiments. The mucilage contained in the golden linseed caused comparatively strong thickening of the product and was broken down by cellulases.
[0119] Samples ultra-high pressure treated at treatment pressures of 1000, 2000, 3000, and 4000 bar were examined.
[0120] The following table summarizes the results of particle size analysis (Malvern Panalytical, Mastersizer 3000), which were performed on the materials that had been treated at the specified pressures. Given below are the parameters d.sub.3.97, d.sub.3.50, and d.sub.3.10, in micrometers, of the volume density distribution of the particles.
TABLE-US-00008 Parameter d.sub.3.97 d.sub.3.50 d.sub.3.10 1000 bar 262 29.5 3.88 2000 bar 123 20.1 3.05 3000 bar 72.6 19.9 4.60 4000 bar 71.2 23.6 5.03
[0121] The following results of a viscosity measurement (Anton Paar Rheometer MCR 102; measuring body: ST-24; temperature=10° C.) show that, at pressures of at least 3000 bar, a significantly higher viscosity was obtained than at 1000 or 2000 bar, while the difference between the samples produced at 3000 bar and at 4000 bar is small.
TABLE-US-00009 Viscosity in mPa .Math. s @ shear rate of 5/s 11.4/s 21/s 49.8/s 101/s 1000 bar 382.6 231.6 163.4 109.2 89.3 2000 bar 702.8 398.3 268.1 164 120.8 3000 bar 1617.9 876.2 565.3 318.2 207.5 4000 bar 1525.2 831.4 548 315.5 205.5
[0122] After a stability test by centrifugation at 4000 g for 10 minutes, the samples prepared at 1000 bar and 2000 bar show less phase separation and more homogeneous sediments than the samples prepared at 3000 bar and 4000 bar.
[0123] It will be apparent to a person skilled in the art that the invention is not limited to the examples described above, but can rather be varied in many ways. More particularly, the features of the individually illustrated examples can also be combined with one another or exchanged for one another.