NEAR-IR EMITTING CATIONIC SILVER CHALCOGENIDE QUANTUM DOTS

Abstract

A novel near-IR emitting cationic silver chalcogenide quantum dot with a mixed coating wherein the coating comprises of at least 2 different types of materials and is capable of luminescence at the desired near IR bandwidth at wavelengths of 800-850 nm. The quantum dot is fabricated via an advantageous single-step, homogeneous, aqueous method at a low temperature resulting a near IR emitting semiconductor quantum dot with high Quantum Yield, high transfection with low toxicity. The quantum dots may be used in medical imaging, tumor detection, drug delivery and labeling as well as in quantum dot sensitized solar cells.

Claims

1. A method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot with a mixed coating, wherein the silver chalcogenide comprises a silver cation source and a sulfide source; wherein the silver chalcogenide is one or more selected from a group consisting of silver sulfide, silver selenide, and silver telluride; wherein the mixed coating comprises at least two types of coating materials, wherein both of the coating materials are bound to a silver chalcogenide surface; and the first type of the coating material is a macromolecule selected from a group of polymers consisting of polyethyleneimine, poly dimethylaminoethyl methacrylate, poly amido amine dendrimers, dendrimers with amine end groups and chitosan; and the second type of the coating material is selected from a group consisting of thiolates, carboxylates and amines; wherein the method is single-step, and takes place in a homogeneous, aqueous environment and at room temperature.

2. The method of synthesizing a near-JR emitting cationic silver chalcogenide quantum dot of claim 1, comprising: i. reacting a water soluble silver salt and a water soluble chalcogenide source in an aqueous medium in the presence of coating materials at room temperature, at a pH-1 value ranging from 5 to 11 under an inert atmosphere to obtain a mixture; ii. stirring the mixture for a crystal growth; and iii. subsequently washing a resulting quantum dot with water.

3. The method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot of claim 1, wherein the silver chalcogenide is silver sulfide.

4. The method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot of claim 1, wherein the first type of the coating material is polyethyleneimine.

5. The method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot of claim 1, wherein the second type of the coating material is 2-mercaptopropionic acid.

6. The method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot of claim 1, wherein the silver chalcogenide is silver sulfide, the first type of the coating material is polyethyleneimine, and the second type of the coating material is 2-mercaptopropionic acid.

7. The method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot of claim 1, wherein the first type of the coating material is a 25 kDa branched polyethyleneimine, and a mole ratio of the polyethyleneimine to the 2-mercaptopropionic acid is from 60/40 to 80/20.

8. The method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot of claim 1, wherein a mole ratio of the silver cation source to the coating material is 1/5.

9. The method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot of claim 1, wherein a mole ratio of the silver cation source to the sulfide source is 4.

10. The method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot of claim 1, wherein a molar ratio of the polyethyleneimine to 2-mercaptopropionic acid is 80/20, wherein a pH-value of a reaction mixture is set to a value of 5.5-11.0.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0019] FIG. 1 The illustration of the cationic silver chalcogenide quantum dot with a binary coating.

[0020] FIG. 2 Aqueous synthesis of PEI and 2-MPA coated Ag.sub.2S NIRQDs.

[0021] FIG. 3 Photoluminescence spectra of Cat-Ag.sub.2S-QDs prepared with different PEI/2MPA ratios (mole of Ag:mole of S=4, Reaction at room temperature and pH 10, reaction time: 5 min).

[0022] FIG. 4 Photoluminescence spectra of Cat-Ag.sub.2S-QDs prepared with PEI/2MPA ratios of 60/40 and 80/20 at pH 7.5, 9 and 10 (mole of Ag:mole of S=4, mole of Ag:mole of PEI=1:3, mole of Ag:mole of 2-MPA=1:2; Reaction at room temperature, reaction time: 5 min).

[0023] FIG. 5 TEM images of Cat-Ag.sub.2S-QDs with 80/20 PEI/2MPA synthesized at pH 9.0 particle distributions a) 20 nm and b 5 nm scale c) diffraction of Ag.sub.2S crystal lattice d) d-spacing between a plane determined by a focused image.

[0024] FIG. 6 Confocal images of HeLa cells treated with Cat-Ag.sub.2S-QDs. Near IR (a), transmission (b) merged image (c) of an individual cell. Red color shows the quantum dots. Arrows show QDs in the cell.

[0025] FIG. 7 MTT assays illustrating the percentage cell viability of HeLa cells exposed free Ag.sup.+ ion, Cat-Ag.sub.2S-QD with 80/20 PEI/2MPA synthesized at pH 9, 2-MPA coated Ag.sub.2S and PEI coated Ag.sub.2S. Concentrations are based on Ag.sup.+ ion concentration in QD solutions. This concentration range corresponds to 4.6-115 g QD/mL.

DETAILED DESCRIPTION OF THE INVENTION

[0026] One aspect of the invention is a near-IR emitting cationic silver chalcogenide quantum dot with a mixed coating wherein the silver chalcogenide is selected from a group comprising silver sulfide (Ag.sub.2S), silver selenide (Ag.sub.2Se), silver telluride (Ag.sub.2Te) and mixtures thereof and the mixed coating comprises of at least two types of materials both are capable of binding to silver chalcogenide surface and one being a macromolecule selected from a group of polymers comprising polyethyleneimine, poly(dimethylaminoethyl methacrylate), Poly(amido amine)dendrimer (PAMAM), dendrimers with amine end groups and chitosan and the other one is being a small molecule selected from a group of small molecules comprising thiolates, carboxylates and amines. In a preferred embodiment of the invention, the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S). In another preferred embodiment of the invention, the macromolecular coating material is selected to be polyethyleneimine (PEI) and more preferably a 25 kDa branched PEI. In another preferred embodiment of the invention the small molecule coating is selected to be a water soluble thiolated small molecule and preferably a propionic acid and most preferably 2-mercaptopropionic acid.

[0027] A preferred embodiment of the invention is a near-IR emitting cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S) with the mixed coating comprising at least 2 types of materials one of which is selected to be polyethyleneimine and the other one is selected to be a thiolated small molecule and preferably is a propionic acid and most probably is 2-mercaptopropionic acid.

[0028] In a preferred embodiment of the invention, a near-IR emitting cationic silver chalcogenide quantum dot wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S) with the mixed coating comprising at least 2 types of materials one of which is selected to be polyethyleneimine and the other one is selected to be a thiolated small molecule and preferably is a propionic acid and most preferably is 2-mercaptopropionic acid.

[0029] More preferred embodiment of this invention is related to a near-IR emitting cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the quantum dot is characterized as the molar ratio of polyetyleneamine (PEI) to 2-mercaptopropionic acid used in the synthesis is ranging between 60/40 to 80/20.

[0030] Another preferred embodiment of the invention is related to a near-IR emitting cationic silver chalcogenide quantum dot (Cat-Ag.sub.2X-QD) as described above wherein the quantum dot is further characterized as the molar ratio of silver cation source to the total coating material used in the synthesis is 1/5.

[0031] Another preferred embodiment of the invention is a near-IR emitting cationic silver chalcogenide quantum dot (Cat-Ag.sub.2X-QD) as described above wherein the quantum dot is further characterized as the molar ratio of silver cation source to the sulfide source used in the synthesis is 4.

[0032] The most preferred embodiment of the invention is a near-IR emitting cationic silver chalcogenide quantum dot (Cat-Ag.sub.2X-QD) as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the quantum dot is characterized as: [0033] i. The molar ratio of polyetyleneamine (PEI) to 2-mercaptopropionic acid used in the synthesis is 80/20 [0034] ii. The molar ratio of silver cation source to the total coating material used in the synthesis is 1/5 and [0035] iii. The molar ratio of silver cation source to sulfide source used in the synthesis is 4.

[0036] Another aspect of the invention is a method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot with a mixed coating wherein the silver chalcogenide is selected from a group comprising silver sulfide (Ag.sub.2S), silver selenide (Ag.sub.2Se), Silver Telluride (Ag.sub.2Te) and mixtures thereof and the mixed coating comprises of at least 2 types of materials both are capable of binding to silver chalcogenite surface and one being a cationic macromolecule selected from a group of polymers comprising polyethyleneimine, poly(dimethylaminoethyl methacrylate), Poly(amido amine) (PAMAM), poly-L-lactic acid (PLLA), dendrimers with amine end groups and chitosan and the other one is being a small molecule selected from a group of small molecules comprising thiolates, carboxylates and amines and the method is characterized as a single-step, homogeneous, aqueous and the method taking place at room temperature.

[0037] Another selected embodiment of the invention is related to method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot as described above, comprising the steps of [0038] i. Reacting water soluble silver salt and water soluble chalcogenide source in an aqueous medium in the presence of the coating materials at room temperature, at a pH ranging from 5-11 under inert atmosphere and [0039] ii. Stirring the mixture for crystal growth and [0040] iii. Subsequently washing the resulting quantum dot with water

[0041] A preferred embodiment of the invention is a method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot with a binary coating as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S).

[0042] Another preferred embodiment of the invention is related to a method of synthesizing a near-IR emitting cationic silver chalcogenide quantum dot with a binary coating wherein the coating materials are selected from at least two types of materials one of which is a cationic polymeric coating and another one is a selected from a group of small molecules with an affinity to silver chalcogenite crystal surface as described above. In a more preferred embodiment of the invention the polymeric coating is selected to be polyethyleneimine and the other coating is selected from a group of small molecules comprising thiolates, carboxylates and amines.

[0043] Another embodiment of the invention is the method of synthesizing the cationic silver chalcogenide quantum dot as described above wherein one of the coating material is a macromolecule selected from a group of polymers comprising polyethyleneimine, poly(dimethylaminoethyl methacrylate), Poly(amido amine) (PAMAM), poly-L-lactic acid (PLLA), dendrimers with amine end groups and chitosan and the other one is selected to be a thiolated small molecule and preferably is a propionic acid and most probably is 2-mercaptopropionic acid.

[0044] Another preferred embodiment of the present invention is the method of synthesizing the cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S) with the mixed coating comprising at least 2 types of materials one of which is selected to be polyethyleneimine and the other one is selected to be a thiolated small molecule and preferably is a propionic acid and most probably is 2-mercaptopropionic acid.

[0045] Another preferred embodiment of the invention is related to a method of synthesizing the cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the said method is characterized as the used polyetyleneamine (PEI) to 2-mercaptopropionic acid mole ratio of is selected to be in between 60/40 to 80/20.

[0046] Also another preferred embodiment of the invention is about a method of synthesizing the cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the method is characterized as the used silver cation source to the total coating material mole ratio is 1/5.

[0047] A specialized embodiment of the invention is about a method of synthesizing the cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the method is characterized as the used silver cation source to sulfide source mole ratio is 4.

[0048] A preferred embodiment of the invention is related to a method of synthesizing the cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the method is characterized as follows: [0049] i. The molar ratio of used polyetyleneamine (PEI) to 2-mercaptopropionic acid is 80/20 [0050] ii. The molar ratio of silver cation source to the total coating materials is set to 1/5 [0051] iii. The molar ratio of silver cation source to sulfide source is 4 and [0052] iv. The pH of the reaction mixture is set to a value between 5.5-11.0.

[0053] The third aspect of the invention is directed to a novel near-IR emitting cationic quantum dot with a mixed coating wherein the silver chalcogenide is selected from a group comprising silver sulfide (Ag.sub.2S), silver selenide (Ag.sub.2Se), Silver Telluride (Ag.sub.2Te) and mixtures thereof and the mixed coating comprises of at least 2 types of materials both are capable of binding to silver chalcogenide surface and one being a macromolecule selected from a group of polymers comprising polyethyleneimine, poly(dimethylaminoethyl methacrylate), Poly(amido amine) (PAMAM), poly-L-lactide acid (PLLA), dendrimers with amine end groups and chitosan and the other one is being a small molecule selected from a group of small molecules comprising thiolates, carboxylates and amine which has improved quantum yield and transfection efficiency and is synthesized via a single step, homogeneous reaction that takes place in aqueous solution at low temperatures and preferably comprising the steps of; [0054] i. Reacting water soluble silver salt and water soluble chalcogenide source in an aqueous medium in the presence of the coating materials at room temperature, at a pH ranging from 5-11 under inert atmosphere and [0055] ii. Stirring the mixture and [0056] iii. Subsequently washing the resulting quantum dot with water

[0057] In a preferred embodiment of the invention the near-IR emitting cationic silver chalcogenide quantum dot synthesized as described above is characterized that the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S).

[0058] Another preferred embodiment of the invention is the near-IR emitting cationic silver chalcogenide quantum dot synthesized as described above wherein one of the coating materials is selected to be polyethyleneimine and the other one is being a small molecule selected from a group of small molecules comprising thiolates, carboxylates and amines.

[0059] Another preferred embodiment of the invention is the near-IR emitting cationic silver chalcogenide quantum dot synthesized as described above wherein one of the coating material is a macromolecule selected from a group of polymers comprising polyethyleneimine, poly(dimethylaminoethyl methacrylate), Poly(amido amine) (PAMAM), poly-L-lactide acid (PLLA), dendrimers with amine end groups and chitosan and the other one is selected to be a thiolated small molecule and preferably a propionic acid and most probably is 2-mercaptopropionic acid.

[0060] Another embodiment of the invention is the near-IR emitting cationic silver chalcogenide quantum dot synthesized as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyethyleneiminepreferably 25 kDa branched polyetyleneimine and the other coating material is selected to be 2-mercaptopropionic acid.

[0061] Another preferred embodiment of the invention is a near-IR emitting cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the quantum dot wherein the quantum dot is characterized as the molar ratio of polyetyleneamine (PEI) to 2-mercaptopropionic acid used in the synthesis is ranging between 60/40 to 80/20. Another embodiment of the invention is the near-IR emitting cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid wherein the quantum dot is further characterized as the molar ratio of silver cation source to the total coating material used in the synthesis is 1/5.

[0062] Another embodiment of the invention is near-IR emitting cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the quantum dot is further characterized as the molar ratio of silver cation source to the sulfide source used in the synthesis is 4. Another embodiment of the invention is the near-IR emitting cationic silver chalcogenide quantum dot as described above wherein the silver chalcogenide is selected to be silver sulfide (Ag.sub.2S), one of the coating material is selected to be polyetyleneiminepreferably 25 kDa branched polyethyleneimine and the other coating material is selected to be 2-mercaptopropionic acid and the quantum dot is characterized as: [0063] i. The molar ratio of polyetyleneamine (PEI) to 2-mercaptopropionic acid used in the synthesis is 80/20 [0064] ii. The molar ratio of silver cation source to the total coating material used in the synthesis is 1/5 and [0065] iii. The molar ratio of silver cation source to sulfide source used in the synthesis is 4. [0066] iv. The pH of the reaction mixture is set to a value between 5.5-11.0

[0067] One final aspect of the invention is the use of the near-IR emitting cationic silver chalcogenide quantum dot with a mixed coating wherein the coating comprises of at least 2 different types of materials as described earlier in sensors, quantum dot sensitized solar cells, fluorescent tagging and medical applications such as medical imaging including tumor detection and labeling, therapy including drug delivery and transfection.

[0068] The near-IR emitting cationic silver chalcogenide quantum dot can especially be used as optical probes, transfection agents and specific disease targeting agents.

[0069] Quantum Dots have great potential in the field of biotechnology and medicine. Broad absorption and narrow emission profiles of quantum dots allow excitation of different quantum dots at a single wavelength but emission from different quantum dots at different wavelengths depending on the crystal size of the QD. This property of QDs is very advantageous for labeling; including peptides, oligonucleotides, cells, tissues and as such. Fluorescent labeling also known as fluorescent tagging is defined as a fluorophoreusually an organic moleculeis being chemically attached to a biomolecule in order for the detection of the said protein, antibody, oligonucleotide, amino acid, etc. Long luminescence lifetime of quantum dots with respect to organic fluorophores, simultaneous excitation of quantum dots emitting at different wavelengths at a single excitation wavelength, higher extinction coefficient make quantum dots good fluorescent tag candidates. Considering the NIR region, lack of organic fluorophores makes NIR quantum dots very attractive for fluorescent tagging.

[0070] These also provide valuable means for optical imaging for tracking and diagnosis. Considering this quantum dot's ability to cross cell membrane is a critical spec for cell labeling and imaging applications. Medical imaging is another application area that quantum dots stated in the current invention can be useful for. Medical imaging is a method of creating images of the human body for diagnostic purposes mostly. Tumor detection is a branch of medical imaging that quantum dots can be used for. Quantum dots can also be used for therapeutic purposes and preferably as disease specific drug delivery vehicles. Small drugs such as chemotherapeutic drugs and/or oligonucleotides can be conjugated to quantum dots. Gene delivery application can also be conducted by the cationic quantum dots which is a process of foreign DNA, siRNA, mRNA introduction to host cells and done for mostly gene therapy and genetic modifications. Quantum dots conjugated with drugs are valuable theranostic materials where conjugation with disease or tissue specific ligands such as molecules, antibodies, peptides, proteins provide site/tissue/disease specific delivery of QDs and hence the therapeutic agent. Fluorescence of theranostic QDs also provide opportunity to monitor the influence of delivered therapeutic agent to the target site with optical imaging. Optical probes as used in this invention can be defined as the chemical tracking devices.

[0071] Quantum Dots can be utilized as optical probes in variety of sensors detecting a chemical, an ion or a process where interaction of quantum dots with the target species enhances or reduces the luminescence of the quantum dot. Broad absorbance range of quantum dots is also useful in quantum dot sensitized solar cell applications. NIR quantum dots have strong absorbance in the visible region which allows enhanced utilization of the solar energy with NIR quantum dots compared to those which absorb the UV and emit in the visible range, such as cadmium chalcogenides. NIR emitting quantum dots as described herein can be used as optical probes for sensors and as sensitizers in solar cells.

Definitions

[0072] Quantum dots as used herein this invention refer to luminescent nanocrystals that are made of semiconductor materials and exhibits quantum confinement effect. Cationic quantum dots refer to quantum dots that have cationic outer surfaces in other words coated with organic materials with cationic nature. Cationic materials refer to cationic polymers in this invention. Cationic polymers that can be used as a coating for cationic quantum dots can be listed as follows: polyethyleneimine, poly (dimethylaminoethyl methacrylate), Poly(amido amine) (PAMAM), poly-L-lactide acid (PLLA), dendrimers with amine end groups and chitosan. An example to the polymers used in this invention is polyethyleneimine (PEI). PEI may be in a linear or branched form; and the molecular weights may range from 1,800-70,000 Da.

[0073] Mixed coating as used herein refers to binary coating and differs from double coating and the resulting quantum dot differs from a core/shell type quantum dots. The coating materials used in this invention can be classified under 2 groups one of which is a cationic polymer also referred as macromolecule or macromolecular coating can be defined as large molecules that are created by polymerization of smaller subunits and the ones that are used in this invention are listed above.

[0074] The other coating material is selected from a group comprising water soluble small molecules with ability to bind silver chalcogenide crystal surface such as thiolates, amines and carboxylates. Small molecules as used here in this invention refer to low molecular weight organic compounds. Examples to these water soluble small molecules suitable for use in the present invention include but not limited to thioglycolic acid, 3-mercaptopropionic acid, 2-mercaptopropionic acid, thioglycerol, glutathione and cystamine.

[0075] Silver chalcogenide mixture as used herein refers to Ag.sub.2S, Ag.sub.2Se and Ag.sub.2Te mixture in alloy form or as physical mixture. Silver chalcogenide mixture may also refer to core/shell type structures comprising of but not limited to silver chalcogenides such as, Ag.sub.2Se/Ag.sub.2S; Ag.sub.2Te/Ag.sub.2Se; Ag.sub.2Te/Ag.sub.2S, Ag.sub.2S/CdS, Ag.sub.2S/AgInS, AgS/CdS/ZnS.

[0076] In this detailed description of the invention, some exemplary references are used for illustrative purposes only therefore it should be understood that the invention is not limited to the scope of these particular embodiments. The terminology used herein is for the purpose of description and not to limit the scope of the invention.

[0077] Transfection as used herein this invention refers to a process in which nucleic acids are delivered into a cell.

General Synthesis Method

[0078] All reactions were performed under an inert atmosphere. Typically, a water soluble silver salt and 0.25 equimolar of chalcogenide source is dissolved separately in deoxygenated water. Desired amounts of the coating materials (mixed coating) are added to the silver solution and pH of the solution was adjusted to desired value using NaOH and CH.sub.3COOH solutions. Chalcogenide solution was added to the silver and coating mixture under vigorous mechanical stirring at 5000 rpm at room temperature (25 C.). During the reaction, samples were taken at different time zones to follow the particle growth. Prepared quantum dot solutions were washed with deionized water using Amicon-Ultra centrifugal filters (30000 Da cut off) and stored in dark at 4 C. Examples of water soluble silver salts suitable for use in the present invention include, but not limited to silver nitrate, silver acetate, silver propionate, silver sulfate, silver butyrate, silver isobutyrate, silver benzoate, silver tartrate, silver salicylate, silver malonate, silver succinate and silver lactate. Examples of chalcogenide sources suitable for use in the present invention include, but not limited to sodium sulfide (Na.sub.2S); Sodium Selenide (Na.sub.2Se); Sodium Telluride (Na.sub.2Te); hydrogen sulfide (H.sub.2S); hydrogen selenide (H.sub.2Se); Hydrogen Telluride (H.sub.2Te); thioacetamide; thioureas; sodium hydrogen telluride (NaHTe); Sodium hydrogen selenide (NaHSe).

General Characterization Methods

[0079] Absorbance spectra of the prepared silver chalcogenide quantum dots were recorded in the 300-1100 nm range and crystal size of QDs were calculated from the absorption onset determined from the absorbance spectrum using Brus equation (eqn. 1):

[00001]$\begin{array}{cc}.Math..Math.E=\frac{{}^2.Math.{}^2}{8.Math.R^{2.Math.}}\left[\frac{1}{m_e}+\frac{1}{m_h}\right]-1.8.Math..Math.\frac{e^2}{{\mathrm{.Math.}}_{{\mathrm{Ag}}_2.Math.S}.Math.4.Math..Math..Math.{\mathrm{.Math.}}_0.Math.R}& \left(1\right)\end{array}$

[0080] Wherein R is the radius of the nanocrystal, m.sub.e (0.286 m0) and m.sub.h (1.096) are the respective effective electron and hole masses for inorganic core, and .sub.Ag2S (5.95) is the dielectric constant and E is the band gap energy difference between the bulk semiconductor and the nanocrystal.

[0081] Fluoresence spectra of QDs were recorded on a home-made device equipped with an amplified silicon detector sensitive over the wavelength range of 400-1100 nm together with a lock-in amplifier. A continuous-wave, frequency-doubled Nd:vanadate laser was used to excite samples at 532 nm. A concave gold reflector and a 0.5-meter Czerny-Turner monochromator was used to collect and image emission. A long-pass filter with a transmission of 90% between 550-1100 nm was used. Data corrected with the spectral response of the detection system.

[0082] Quantum yield of QDs were calculated according to the formula provided below: s=r (Is/Ir) (Ar/As) (ns/nr), where s, Is, As and ns are the QY, emission peak area, integrated absorption intensity and

refractive index of QDs, respectively, and r, Ir, Ar and nr are the corresponding parameters of LDS 798 NIR dye in methanol (quantum yield reported as 14% by the producer). For QY calculations five different solutions of QD and reference dye were prepared at five different concentrations.

[0083] TEM analysis of nanoparticles was performed using a JEOL JEM-ARM200CFEG UHR-Transmission Electron Microscope (JEOL, Japan). Hydrodynamic size and zeta potential of the aqueous nanoparticles were measured with a Malvern Zetasizer Nano-ZS.

[0084] Quantum Dots were digested with 65% nitric acid-96% sulphuric acid mixture and diluted to certain volumes. Ag.sup.+ ion concentrations in solutions were measured by ICP-OES and calculated using a standard curve of known Ag.sup.+ ion concentrations.

In Vitro Tests

[0085] HeLa cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum and antibiotics (Penicillin/Streptomycin) in a 5% CO.sub.2-humidified incubator at 37 C. 50000 HeLa cells were cultured in dishes and incubated for 18 h. After incubation, culture medium was replenished and cells were incubated with Quantum Dot solution with 2.5 g/mL Ag.sup.+ ion concentration for 6 hours. Cells were washed with PBS (pH 7.2) and fixed with 4% para-formaldehyde for 15 minutes. A home-made confocal microscope system equipped with a 60 (NA:1.49) oil immersion objective and a Si detector was used to image QD internalization by the cells. Briefly, cells were placed on the stage of the confocal laser scanning microscope and exited at 532 nm by laser.

EXAMPLES

[0086] The following examples are provided to illustrate the present invention and are not intended to limit the scope of the invention. Table 1 lists reaction parameters for the synthesis of PEI coated Ag.sub.2S QD, 2MPA coated Ag.sub.2S QD, cationic Ag.sub.2S QDs with mixed coating at pH 10 and properties of the resulting Ag.sub.2S QDs. Table 2 lists properties of cationic silver sulfide quantum dots at different pH values.

Example 1

General Synthesis Method and Particle Characterization of Polyethyleneimine (PEI)/2-Mercaptopropionic Acid Coated Silver Sulfide (Ag.SUB.2.S) (Cat-Ag.SUB.2.S-QD) Nanoparticles (as Shown in FIG. 2)

[0087] All reactions were performed under an inert atmosphere. Typically, PEI and 2-MPA were added to an aqueous solution of AgNO.sub.3 (0.25 mmol in 75 mL deoxygenated MilliQ water) and pH of the solution was adjusted to desired value using NaOH and CH.sub.3COOH solutions (2.5 M). Na.sub.2S (0.0625 mmol in 25 ml of deoxygenated water) solution was added to the PEI/2MPA/AgNO.sub.3 solution under vigorous mechanical stirring at room temperature (25 C.) (Scheme 1.) Samples were withdrawn from the reaction mixture for the assessment of particle growth via UV-Vis spectrophotometer and spectrofluorometer. Usually, crystal growth stops in 5 min, therefore, all comparative reactions were quenched in liquid nitrogen (after 5 minute crystal growth, washed with deionized water using Amicon-Ultra centrifugal filters (30000 Da cut off) and stored in dark at 4 C.

[0088] The prepared Cat-Ag.sub.2S-QDs have PEI and 2-MPA as coating material on the surface. The ratio between the PEI and 2-MPA was studied to obtain stable nanoparticles with high quantum efficiency. PEI to 2-MPA ratio was changed as 100/0, 90/10, 80/20, 60/40, 0/100 (Table 1). FIG. 3 is the normalized absorbance graph of the prepared Cat-Ag.sub.2S-QDs. As depicted from the figure, the best PEI/2-MPA ratios are 60/40 and 80/20.

[0089] At the 60/40 and 80/20 PEI/2-MPA ratios reactions were carried out also pH 5.5, 7.5, 9 and 11. Best luminescence was obtained at pH 10 with 60/40 PEI/2MPA composition and pH 9 for 80/20 PEI/2MPA (FIG. 4). In addition, emission peaks with 60/40 PEI/2MPA of Cat-Ag.sub.2S-QDs has two emission maximum and have poor colloidal stability at pH values below 9. However, Cat-Ag.sub.2S-QDs with 80/20 PEI/2MPA is stable and have strong emission.

[0090] Properties of Cat-Ag.sub.2S-QDs with binary coating as described herein influenced by the pH of the medium, as well. Table 2 shows the changes in particle properties of Cat-Ag.sub.2S-QDs synthesized with PEI/2MPA ratio of 80/20 at pH9 and at room temperature with the pH after synthesis and purification. At pH 7.4 quantum yield is doubled compared to pH 9 and reached to 166% at pH 5.5.

[0091] TEM images of the particles reveal mostly spherical particles with sizes around 2-4 nm (FIG. 5).

TABLE-US-00001 TABLE 1 Effect of PEI/2-MPA ratios on the properties of Cat-Ag.sub.2S-QDs Re- 2- ac- Band Zeta PEI MPA tion .sub.abs(cutoff).sup.a Size.sup.b gap .sub.em(max) FWHM, Dh.sup.c pot. (%) (%) pH (nm) (nm) (eV) (nm) nm (nm) (mV) 100 0 10 906 2.94 1.37 4.0 51 90 10 10 674 2.23 1.48 838 175 2.9 34 80 20 10 761 2.48 1.63 819 173 3.5 28 60 40 10 777 2.52 1.60 817 168 3.8 36 0 100 7.5 806 2.61 1.54 837 128 7.10 62 .sup.aAbsorbance onset. .sup.bCalculated by Brus equation. .sup.cHydrodynamic diameter measured by DLS at pH 7.4 and reported as the number average.

TABLE-US-00002 TABLE 2 Influence of pH on the properties of Ag.sub.2S-PEI/2MPA Cat-Ag.sub.2S-QDs* Band Zeta .sub.abs(cutoff).sup.a Size.sup.b gap .sub.em(max) FWHM, Dh.sup.c pot. QY pH (nm) (nm) (eV) (nm) nm (nm) (mV) (%) 5.5 783 2.54 1.59 812 151 9.4 63 166 7.4 783 2.54 1.59 828 150 8.9 60 150 9.0 783 2.54 1.59 825 170 8.0 32 77 .sup.aAbsorbance onset. .sup.bCalculated by Brus equation. .sup.cHydrodynamic diameter measured by DLS and reported as the number average. .sup.dQuantum yield calculated with respect to LDS 798 near-IR dye (Ag:S = 4, Ag:PEI = 1:4, Ag:2-MPA = 1:1, Temp = RT, reaction pH = 9, 5 min reaction). *Cationic Ag.sub.2S quantum dots synthesized with 80% PEI, 20% 2-MPA at pH 9.0 were used.

Example 2

Synthesis Method of Polyethyleneimine (PEI)/2-Mercaptopropionic Acid Coated Silver Sulfide (Ag.SUB.2.S) Nanoparticle with 60/40 Molar Ratio of PEI/2-Mercaptopropionic Acid

[0092] 2.1410.sup.3 mmol PEI (0.75 mmol NH.sub.2) and 0.5 mmol 2-MPA (0.5 mmol SH) were added to an aqueous solution of AgNO.sub.3 (0.25 mmol in 75 mL deoxygenated MilliQ water) and pH of the solution was adjusted to 10. Na.sub.2S (0.0625 mmol in 25 ml of deoxygenated water) solution was added to the PEI/2MPA/AgNO.sub.3 solution under vigorous mechanical stirring at room temperature (25 C.) (Samples withdrawn from the reaction mixture for the assessment of particle growth via UV-Vis spectrophotometer and spectrofluorometer.) QDs washed with deionized water using Amicon-Ultra centrifugal filters (30000 Da cut off) and stored in dark at 4 C.

Example 3

Synthesis Method of Polyethyleneimine (PEI)/2-Mercaptopropionic Acid Coated Silver Sulfide (Ag.SUB.2.S) Nanoparticle with 80/20 Molar Ratio of PEI/2-Mercaptopropionic Acid

[0093] 2.85610.sup.3 mmol PEI (1 mmol NH.sub.2) and 0.25 mmol 2-MPA (0.25 mmol SH) were added to an aqueous solution of AgNO.sub.3 (0.25 mmol in 75 mL deoxygenated MilliQ water) and pH of the solution was adjusted to 9. Na.sub.2S (0.0625 mmol in 25 ml of deoxygenated water) solution was added to the PEI/2MPA/AgNO.sub.3 solution under vigorous mechanical stirring at room temperature (25 C.) (Samples withdrawn from the reaction mixture for the assessment of particle growth via Uv-vis spectrophotometer and spectrofluorometer.) QDs washed with deionized water using Amicon-Ultra centrifugal filters (30000 Da cut off) and stored in dark at 4 C.

Example 4

In Vitro Cell Internalization Studies for Polyethyleneimine (PEI)/2-Mercaptopropionic Acid Coated Silver Sulfide (Ag.SUB.2.S) Nanoparticles

[0094] Ag.sub.2S quantum dots synthesized with 80/20 molar ratio of PEI/2MPA as described in example 3 was used for in vitro cell internalization and optical imaging of QDs in cells. Briefly, 50000 HeLa cells were cultured in dishes and incubated for 18 h. After incubation, culture medium was replenished and cells were incubated with Quantum Dot solution with 2.5 g/mL Ag.sup.+ ion concentration for 6 hours. Cells were washed with PBS (pH 7.2) and fixed with 4% para-formaldehyde for 15 minutes. A home-made confocal microscope system equipped with a 60 (NA: 1.49) oil immersion objective and a Si detector was used to image QD internalization by the cells. Briefly, both QD treated and untreated cells were placed on the stage of the confocal laser scanning microscope and exited at 532 nm by laser. A significant emission was detected from cells treated with QDs however, no emission, no autofluoresence was detected from the untreated cells in the equal scale of emission intensity. The study proves the efficient uptake of QDs and effectiveness of the NIR emission at in the NIR in cell imaging with no complication of autofluoresence from cells (FIG. 6).

Example 5

Cytotoxicity Tests for Polyethyleneimine (PEI)/2-Mercaptopropionic Acid Coated Silver Sulfide (Ag.SUB.2.S) Nanoparticles

[0095] For the cytotoxicity assessment HeLa cells were cultured in the 96-well plates in complete medium at 37 C. and 5% CO.sub.2 for 24 h. On the second day, medium was renewed and Qunatum Dots were added to the culture medium at 1-25 g Ag.sup.+/mL concentrations and incubated for another 24 h. On the third day, cells were washed with PBS. MTT (thiazolyl blue tetrazolium bromide (3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide) solution was added on the cells and incubated for 4 h. Purple formazan was dissolved with DMSO:Ethanol (1:1) by gentle shaking for 15 min. Absorbance of formazan was measured at 600 nm with a reference at 630 nm on a microplate reader. Qunatum Dot absorbance in complete medium was measured as well and subtracted from MTT solution for the correction. Cytocompatibility of 100% polyethyleneimine coated, 100% 2-mercaptopropionic acid coated and 80/20 PEI/2MPA coated Ag.sub.2S Qunatum Dots were tested on HeLa cells at 1-25 g Ag.sup.+/mL concentrations (FIG. 7). The toxicity of free Ag.sup.+ ion and PEI were also measured for comparison. At this concentration range free Ag.sup.+ does not cause any significant cell death which is advantageous as compared to Cadmium; Mercury and Lead. The results can be summarized as follows: 100% 2MPA coated Ag.sub.2S QDs are not toxic; polyethyleneimine is highly toxic even at 2.8 g/mL PEI level; but polyetyleneimine cytotoxicity diminishes dramatically as it binds to Ag.sub.2S surface; both Ag.sub.2S with 100% PEI and 80/20 PEI/2MPA showed no significant drop in the cell viability.