SEPARATING AGENT FOR OPTICAL ISOMERS

Abstract

Provided is a separating agent for optical isomers, which is configured of a novel xylan derivative and a carrier. Specifically provided is a separating agent for optical isomers, which is configured of a carrier and a xylan-phenylcarbamate derivative that is obtained by substituting a hydroxy group of xylan with a group represented by formula (I) or (II). (In formula (I), R1 represents a halogen or an alkyl group having 1-5 carbon atoms; and the position of substitution of the R1 moiety is the meta position or the para position. In formula (II), each of R2 and R3 independently represents a halogen or an alkyl group having 1-5 carbon atoms, and R2 and R3 are groups different from each other.)

Claims

1. A separating agent for optical isomers, comprising a carrier, and a xylan-phenyl carbamate derivative in which hydroxyl groups in the xylan are substituted with groups represented by formula (I) or formula (II) below: ##STR00010## (in formula (I), R.sub.1 is a halogen or an alkyl group having 1 to 5 carbon atoms, and the R.sub.1 substitution position is a meta position or a para position; in formula (II), R.sub.2 and R.sub.3 are each independently a halogen or an alkyl group having 1 to 5 carbon atoms, and R.sub.2 and R.sub.3 are different from each other).

2. The separating agent for optical isomers according to claim 1, wherein the groups that substitute the hydroxyl groups in the xylan are groups represented by formula (I), and R.sub.1 is an alkyl group having 1 to 5 carbon atoms.

3. The separating agent for optical isomers according to claim 2, wherein the R.sub.1 substitution position is a meta position, and R.sub.1 is a methyl group or an ethyl group.

4. The separating agent for optical isomers according to claim 1, wherein the groups that substitute the hydroxyl groups in the xylan are groups represented by formula (II), and in formula (II), R.sub.2 is a halogen and R.sub.3 is an alkyl group having 1 to 5 carbon atoms.

5. The separating agent for optical isomers according to claim 4, wherein R.sub.2 is chlorine and R.sub.3 is a methyl group or an ethyl group.

6. The separating agent for optical isomers according to claim 1, wherein the carrier is silica gel.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0020] FIG. 1 is a diagram that shows a .sup.1H-NMR spectrum of a xylan-3-methylphenyl carbamate derivative at 80 C. in DMSO-d.sub.6.

[0021] FIG. 2 is a diagram that shows a chromatogram obtained by separating a specific racemate using the separating agent for optical isomers produced in Example 1.

[0022] FIG. 3 is a diagram that shows chromatograms obtained by separating a specific racemate using the separating agents for optical isomers produced in Examples 2 and 4 and Comparative Example 3.

DESCRIPTION OF EMBODIMENTS

[0023] The separating agent for optical isomers of the present invention is constituted from a carrier and a xylan-phenyl carbamate derivative in which hydroxyl groups in the xylan are substituted with groups represented by formula (I) or formula (II) below.

##STR00002##

(In formula (I), R.sub.1 is a halogen or an alkyl group having 1 to 5 carbon atoms, and the R.sub.1 substitution position is a meta position or a para position.)

##STR00003##

(In formula (II), R.sub.2 and R.sub.3 are each independently a halogen or an alkyl group having 1 to 5 carbon atoms, and R.sub.2 and R.sub.3 are different from each other.)

[0024] The xylan used in the present invention may be -1, 4-xylan, -1,3-xylan, or a mixture of these.

[0025] The average degree of polymerization of the xylan (the average number of pyranose rings in 1 molecule of xylan) is preferably 5 or more, and more preferably 10 or more, with no particular upper limit, but this average degree of polymerization is preferably 1000 or less from the perspective of ease of handling, and is more preferably 5 to 1000, further preferably 10 to 1000, and particularly preferably 10 to 500.

[0026] In the xylan-phenyl carbamate derivative, some of the hydroxyl groups in the xylan may be unreacted, and may be substituted with another substituent group as long as the advantageous effect of the present invention is not impaired. A mode in which such hydroxyl groups account for approximately 20% or less of all the hydroxyl groups can be given as an example.

[0027] A method such as that described below can be given as an example of a method of substituting hydrogen atoms in hydroxyl groups in xylan with substituted phenyl carbamate groups represented by formula (I) or formula (II).

[0028] A phenylisocyanate compound in which a hydrogen atom on the aromatic ring is substituted with R.sub.1, as shown in formula (I) above, or a phenylisocyanate compound substituted with R.sub.2 and R.sub.3, as shown in formula (II) above, is dissolved in an amide-based solvent, such as N,N-dimethylacetamide, N-methyl-2-pyrrolidone or 1,3-dimethyl-2-imidazolidinone, in which lithium chloride is dissolved, xylan is added to this solution, and a reaction is brought about at an appropriate temperature for an appropriate duration (20 to 100 C., 1 to 24 hours), thereby causing the isocyanate group in the phenylisocyanate compound to react with a hydroxyl group in the xylan.

[0029] In addition to the amide-based solvent, pyridine, for example, can be used at the same time.

[0030] In formula (I) above, chlorine, bromine or fluorine can be used as the halogen, and chlorine is preferred. In addition, a methyl group or an ethyl group is preferred as the alkyl group having 1 to 5 carbon atoms.

[0031] In addition, R.sub.1 is positioned at a meta position or a para position, but the meta position is advantageous in terms of optical resolution of specific optical isomers.

[0032] In addition, it is preferable for R.sub.1 to be an alkyl group having 1 to 5 carbon atoms from the perspective of excellent optical resolution of specific optical isomers.

[0033] In formula (II), chlorine, bromine or fluorine can be used as the halogen, and chlorine is preferred. In addition, a methyl group or an ethyl group is preferred as the alkyl group having 1 to 5 carbon atoms.

[0034] R.sub.2 and R.sub.3 are different from each other, and in cases where one of R.sub.2 or R.sub.3 is a halogen, it is preferable for the other of R.sub.2 or R.sub.3 to be an alkyl group having 1 to 5 carbon atoms from the perspective of excellent optical resolution of specific optical isomers.

[0035] Specifically, it is preferable for chlorine to be substituted as a halogen in one of R.sub.2 or R.sub.3 and for a methyl group or an ethyl group to be substituted as a group that is not a halogen. For example, R.sub.2 is preferably a halogen, and more preferably chlorine. In addition, R.sub.3 is preferably an alkyl group having 1 to 5 carbon atoms, and more preferably a methyl group.

[0036] Examples of carriers used to support the xylan derivative include porous organic carriers and porous inorganic carriers, with porous inorganic carriers being preferred. Suitable porous organic carriers include polymeric substances selected from among polystyrene, poly(meth)acrylamide, poly(meth)acrylates, or the like and suitable porous inorganic carriers include silica gel, alumina, zirconia, titania, magnesia, glass, kaolin, titanium oxide, silicate, hydroxyapatite, or the like. Preferred carriers are silica gel, alumina and glass.

[0037] It is possible to suppress excessive adsorption of a substance to be separated on the carrier by subjecting the carrier to a surface treatment. Examples of surface treatment agents include silane coupling agents such as aminopropylsilane and titanate-based and aluminate-based coupling agents.

[0038] The average particle diameter of a carrier able to be used in the present invention is generally 0.1 to 1000 m, and preferably 1 to 50 m. The average pore diameter of the carrier is generally 10 to 10000 , and preferably 50 to 1000 .

[0039] In addition, the specific surface area of the carrier is generally 5 to 1000 m.sup.2/g, and preferably 10 to 500 m.sup.2/g.

[0040] The average particle diameter of the separating agent for optical isomers of the present invention can be measured using an apparatus in which measurements are carried out using microscope images, such as a Mastersizer 2000E available from Malvern Instruments Ltd.

[0041] In the separating agent for optical isomers of the present invention, the quantity of the xylan-phenyl carbamate derivative supported on the carrier can be 1 to 100 wt. %, and preferably 5 to 50 wt. %, relative to the carrier.

[0042] This supported quantity can be determined by means of thermogravimetric analysis.

[0043] The method for supporting the xylan-phenyl carbamate derivative on the carrier may be a chemical method or a physical method. Examples of physical methods include a method of dissolving the xylan-phenyl carbamate derivative in a solvent capable of dissolving same, mixing thoroughly with the carrier, and then removing the solvent by means of reduced pressure, heating or an air stream, and a method of dissolving the xylan-phenyl carbamate derivative in a solvent capable of dissolving same, mixing thoroughly with the carrier, and then dispersing in a solvent in which the xylan-phenyl carbamate derivative is insoluble so as to diffuse the solvent in which the xylan-phenyl carbamate derivative is soluble. A separating agent obtained in this way may be subjected to a suitable treatment, such as heating, addition of a solvent or washing, so as to improve separation performance.

[0044] The separating agent for optical isomers of the present invention can be packed in a column having a publicly known size by means of a publicly known method, and used as an HPLC column.

[0045] When separating optical isomers by means of HPLC using a column packed with the separating agent for optical isomers of the present invention, the HPLC flow rate may be adjusted as appropriate, and an example thereof is 0.1 to 5 mL/min.

[0046] In addition to HPLC, the separating agent for optical isomers of the present invention can be used as a filler for a capillary column for gas chromatography or electrophoresis, and especially for capillary electrochromatography (CEC), CZE (capillary zone electrophoresis) and MEKC (micellar electrokinetic chromatography).

EXAMPLES

[0047] The present invention will now be explained in greater detail through the use of examples. However, the present invention is not limited to the examples given below.

Example 1

[0048] A pre-treatment was carried out by dispersing xylan derived from beech wood (available from Sigma-Aldrich) in water at 60 C. and recovering xylan as an acetone-insoluble fraction.

[0049] Xylan-phenyl carbamate derivative (a), into which meta-methylphenyl carbamate groups represented by formula (1) below were introduced, was obtained by reacting the pre-treated xylan with an excess of phenylisocyanate in which the meta position was substituted with a methyl group in a solution (dimethylacetamide, lithium chloride, dry pyridine) at 80 C. according to the reaction formula shown below, so as to convert hydroxyl groups in the xylan into carbamoyl groups.

##STR00004##

[0050] A .sup.1H-NMR spectrum of the obtained xylan-phenyl carbamate derivative is shown in FIG. 1. The .sup.1H-NMR spectrum (500 MHz) was obtained using a Brucker-500 Spectrometer (available from Brucker, USA).

##STR00005##

[0051] Table 1 shows results obtained from thermogravimetric analysis of a xylan-phenyl carbamate derivative substituted with substituted phenyl carbamate groups represented by formula (1).

TABLE-US-00001 TABLE 1 Measured value C %: 64.47 H %: 6.59 N %: 6.54 Theoretical value C %: 64.25 H %: 6.54 N %: 6.32

Examples 2 to 7

[0052] Xylan-phenyl carbamate derivatives (b) to (g), into which substituted phenyl carbamate groups represented by formulae (2) to (7) below were introduced, were obtained using the same procedure as that used in Example 1, except that phenylisocyanates having different substituent groups were used.

##STR00006##

Comparative Examples 1 to 4

[0053] Xylan-phenyl carbamate derivatives (h) to (k) of Comparative Examples 1 to 4, into which substituted phenyl carbamate groups represented by formulae (8) to (12) below were introduced, were obtained using the same procedure as that used in Example 1, except that phenylisocyanates having different substituent groups were used.

##STR00007##

[0054] .sup.1H NMR data (DMSO-d6, 80 C.) for the xylan-phenyl carbamate derivatives produced in the examples and comparative examples are given below. Xylan-phenyl carbamate derivatives (j) and (k) are publicly known, and data for these are therefore omitted.

[0055] (b): .sup.1H NMR (DMSO-d6): 3.1-5.7 (glucose protons, 6H), 6.6-7.9 (aromatic, 8H), 9.2-10.1 (NH, 2H).

[0056] (c): .sup.1H NMR (DMSO-d6): 3.2-5.3 (glucose protons, 6H), 6.6-7.7 (aromatic, 8H), 9.0-9.8 (NH, 2H).

[0057] (d): .sup.1H NMR (DMSO-d6): 3.1-5.2 (glucose protons, 6H), 6.9-7.8 (aromatic, 8H), 9.1-9.9 (NH, 2H).

[0058] (e): .sup.1H NMR (DMSO-d6): 1.8-2.5 (CH.sub.3, 6H), 3.1-5.3 (glucose protons, 6H), 6.6-7.6 (aromatic, 8H), 8.7-9.6 (NH, 2H).

[0059] (f): .sup.1H NMR (DMSO-d6): 0.7-1.4 (CH.sub.3, 6H), 2.1-2.7 (CH.sub.2, 4H), 3.1-5.4 (glucose protons, 6H), 6.6-7.8 (aromatic, 8H), 8.7-9.8 (NH, 2H).

[0060] (g): .sup.1H NMR (DMSO-d6): 1.9-2.4 (CH.sub.3, 6H), 3.1-5.3 (glucose protons, 6H), 6.8-7,9 (aromatic, 6H) 9.1-10.0 (NH, 2H).

[0061] (h): .sup.1H NMR (DMSO-d6): 3.1-5.6 (glucose protons, 6H), 7.2-8.5 (aromatic, 8H), 9.7-10.5 (NH, 2H).

[0062] (i): .sup.1H NMR (DMSO-d6): 3.4-4.0 (CH.sub.3, 6H), 3.1-5.2 (glucose protons, 6H), 6.3-7.5 (aromatic, 8H), 8.6-9.4 (NH, 2H).

Preparation of Separating Agents for Optical Isomers, and Production of Analytical Columns

[0063] Separating agent for optical isomers-1 was obtained by completely dissolving 0.35 g of xylan-phenyl carbamate derivative (a) prepared in Example 1 in 8 mL of tetrahydrofuran, and then coating the obtained solution on the surface of 1.40 g of silica gel (average particle diameter 7 m, average pore diameter 100 nm) that had been surface treated in advance with aminopropyltriethoxysilane.

[0064] Column-1 was obtained by packing separating agent for optical isomers-1 in a stainless steel column (25 cm0.20 cm i.d.) using a slurry method.

[0065] The number of theoretical plates in column-1 was 1500 to 3000 when using benzene as a reference, using a mixture of hexane/2-propanol (90/10, v/v) as an eluant and setting the flow rate to 0.1 mL/min.

[0066] The dead time (t.sub.0) was measured using 1,3,5-tri-tert-butylbenzene as a non-retentive substance.

[0067] A JASCO PU-2089 chromatograph equipped with a UV/Vis (Jasco UV-2070) and a circular dichroism detector (JASCO CD-2095) was used as the HPLC apparatus.

[0068] A sample (a 2 mg/mL solution of a racemate) was injected into the chromatograph system using an Intelligent sampler (JASCO AS-2055).

[0069] FIG. 2 shows a chromatogram obtained by separating a racemate having the following structure.

##STR00008##

[0070] Separating agents for optical isomers-2 to -11 were obtained in the same way as separating agent for optical isomers-1 using xylan-phenyl carbamate derivatives (b) to (k) obtained in Examples 2 to 7 and Comparative Examples 1 to 4. In addition, these separating agents for optical isomers were packed in stainless steel columns in the same way as in Example 1.

[0071] Racemates 1 and 2 having the structures shown below were separated using columns packed with separating agents for optical isomers-1 to -11. When carrying out analysis using these columns, hexane/2-propanol (90/10, v/v) was used as an eluant, the flow rate was 0.1 mL/min, and the detection wavelength was 254 nm.

##STR00009##

[0072] Tables 2 and 3 show results obtained by separating racemates 1 and 2 using the columns.

[0073] The asymmetry identification ability (separation coefficient ) of the compounds shown in the tables was calculated from the retention coefficient (k.sub.1) in the manner shown below.

[0074] In addition, FIG. 3 shows chromatograms obtained by separating racemate 1 by means of columns using the separating agents for optical isomers of Examples 2 and 4 and Comparative Example 3.

[0075] Retention Coefficient (k.sub.1)

k.sub.1=[(enantiomer retention time)(dead time)]/dead time

[0076] Separation Coefficient (a)

=(retention coefficient of more strongly retained enantiomer)/(retention coefficient of more weakly retained enantiomer)

TABLE-US-00002 TABLE 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 a: 3-CH.sub.3 b: 3-Cl c: 4-F d: 4-Cl e: 4-CH.sub.3 f: 4-C.sub.2H.sub.5 g: 3-Cl-4-CH.sub.3 Racemate k.sub.1 k.sub.1 k.sub.1 k.sub.1 k.sub.1 k.sub.1 k.sub.1 1 0.32(+) 1.38 0.40(+) 1.35 0.37(+) 1.36 0.39(+) 1.25 0.36(+) 1.46 0.33(+) 1.46 0.31(+) 1.29 2 1.30() 1.62 0.88() 1.4 1.24() 1.51 0.94() 1.31 1.99() 1.44 1.71() 1.61 1.02() 1.37 *In the table, the symbols in parentheses indicate the optical rotation at 254 nm of the initially eluted enantiomer.

TABLE-US-00003 TABLE 3 Comp. Comp. Ex. 4 Comp. Ex. 1 Comp. Ex. 2 Ex. 3 k: 3,5- Race- h: 4-NO.sub.2 i: 4-OCH.sub.3 j: (3,5-Cl.sub.2) (CH.sub.3).sub.2 mate k1 k1 k1 k1 1 0.35(+) 1.15 0.48(+) 1.22 0.51(+) 1.19 0.47(+) 1.40 2 1.08 1.0 2.94 1.0 0.45() 1.23 1.44() 1.42 *In the table, the symbols in parentheses indicate the optical rotation at 254 nm of the initially eluted enantiomer.

[0077] From the results in Tables 2 and 3, it was understood that separating agents for optical isomers of Examples 1 to 6, which had non-polar substituent groups, exhibited higher separation coefficients for racemates 1 and 2 than Comparative Examples 1 and 2, which had polar substituent groups.

[0078] In view of the separation results for racemate 2 in Table 2 and Table 3, Examples 1 and 5, in which only 1 methyl group was substituted, exhibited higher separation coefficients than Comparative Example 4, in which 2 methyl groups were substituted.

[0079] In addition, Example 6, in which the para position was substituted with an ethyl group, exhibited similar separative ability to Examples 1 and 5 for racemates 1 and 2.

[0080] Among the separating agents for optical isomers in Table 2 and Table 3, comparing those in which the phenyl group was substituted with chlorine showed that the separation coefficient for racemates 1 and 2 was such that Example 2 in which one meta position was substituted with chlorine>Example 4 in which the para position was substituted with chlorine>Example 7 in which a meta position was substituted with chlorine and the para position was substituted with a methyl group>Comparative Example 3 in which two meta positions were substituted with chlorine.

[0081] Comparing Examples 1 and 5 with Examples 2 and 4 in Table 2, it was understood that compounds in which meta positions of the phenyl group were substituted exhibited better separative ability of racemates 1 and 2.

[0082] In addition, comparing Example 2 and Example 1 in Table 2 and comparing Comparative Example 3 and Comparative Example 4 in Table 3, it was understood that compounds having a methyl group, which is an electron-donating group, exhibited better separative ability of racemates 1 and 2 than compounds having chlorine, which is an electron-withdrawing group.

INDUSTRIAL APPLICABILITY

[0083] According to the present invention, it is possible to provide a separating agent for optical isomers, which uses a xylan-phenyl carbamate derivative and which exhibits better separative ability for specific racemates than xylan-phenyl carbamate derivatives in which hydroxyl groups in xylan are substituted with phenyl carbamate groups substituted with chlorine atoms or methyl groups at the 3- and 5-positions of the phenyl group.