MIXTURES OF SABADILLA ALKALOIDS AND N-OCTYL BICYCLOHEPTENE DICARBOXIMIDE AND USES THEREOF

Abstract

The present invention is directed to pesticidal mixtures comprising sabadilla alkaloids and N-octyl bicycloheptene dicarboximide and methods of controlling pests including insects and mites by application of pesticidal mixtures comprising sabadilla alkaloids and N-octyl bicycloheptene dicarboximide.

Claims

1. A pesticidal mixture comprising an effective amount of sabadilla alkaloids and N-octyl bicycloheptene dicarboximide.

2. The mixture of claim 1, wherein the sabadilla alkaloids are derived from Schoenocaulon officinale.

3. The mixture of claim 1, wherein the sabadilla alkaloids are veratridine and cevadine.

4. The mixture of claim 1, wherein the ratio of sabadilla alkaloids to N-octyl bicycloheptene dicarboximide is from about 1:2.5 to about 25:1.

5. The mixture of claim 1, wherein the ratio of sabadilla alkaloids to N-octyl bicycloheptene dicarboximide is from about 1:1 to about 1:10.

6. The mixture of claim 1, wherein the sabadilla alkaloids are at a concentration from about 0.05% to about 0.5% w/w, wherein w/w denotes weight by total weight of the mixture.

7. The mixture of claim 1, wherein the N-octyl bicycloheptene dicarboximide is at a concentration from about 0.05% to about 5.0% w/w, wherein w/w denotes weight by total weight of the mixture.

8. The mixture of claim 1 further comprising one or more excipients selected from the group consisting of solvents, anti-caking agents, stabilizers, defoamers, slip agents, humectants, dispersants, wetting agents, thickening agents, emulsifiers, penetrants, adjuvants, polymers, propellants and/or preservatives.

9. A method of controlling a pest comprising applying a pesticidal mixture comprising an effective amount of sabadilla alkaloids and N-octyl bicycloheptene dicarboximide to the pest or the pest's environment.

10. The method of claim 9, wherein the pest is at least one of an insect and a mite.

11. The method of claim 9, wherein the pest is selected from the group consisting of bed bugs (Hemiptera), fleas (Siphonaptera), cockroaches (Blattodea), flies (Diptera), ants (Hymenoptera), mosquitoes (Culicidae) and mites (Acari).

12. The method of claim 9, wherein the pest is selected from the group consisting of common bed bugs (Cimex lectularius), house fly (Musca domestica), yellow fever mosquito (Aedes aegypti), southern house mosquito (Culex quinquefasciatus), African malaria mosquito (Anopheles gambiae), common malaria mosquito (Anopheles quadrimaculatus) and German cockroach (Blattella germanica).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0011] Applicant unexpectedly discovered that pesticidal mixtures of sabadilla alkaloids and N-octyl bicycloheptene dicarboximide provided enhanced pesticidal activity. Further, Applicant discovered that pesticidal mixtures of sabadilla alkaloids and N-octyl bicycloheptene dicarboximide were capable of controlling a large variety of arthropods.

[0012] The present invention is directed to pesticidal mixtures comprising an effective amount of sabadilla alkaloids and N-octyl bicycloheptene dicarboximide.

[0013] Sabadilla alkaloids may be derived from any species of Schoenocaulon. The genus Schoenocaulon includes the following species: S. calcicola, S. caricifolium, S. comatum, S. conzattii, S. dubium (alt. S. gracile), S. framei, S. ghiesbreghtii (alt. S. drummondii, S. yucatanense), S. ignigenum, S. intermedium, S. jaliscense, S. macrocarpum (alt. S. lauricola), S. madidorum, S. megarrhizum, S. mortonii, S. oaxacense, S. obtusum, S. officinale, S. pellucidum, S. plumosum, S. pringlei, S. rzedowskii, S. tenorioi, S. tenue, S. tenuifolium, S. texanum, and S. tigrense. In a preferred embodiment the sabadilla alkaloids are derived from S. officinale. In another preferred embodiment, the sabadilla alkaloids are veratridine and cevadine.

[0014] N-octyl bicycloheptene dicarboximide is a synthetic chemical having the following chemical structure:

##STR00001##

[0015] As used herein, all numerical values relating to amounts, weight percentages and the like are defined as about or approximately each particular value, namely, plus or minus 10%. For example, the phrase at least 5% by weight is to be understood as at least 4.5% to 5.5% by weight. Therefore, amounts within 10% of the claimed values are encompassed by the scope of the claims.

[0016] As used herein, w/w denotes weight by weight of the total mixture.

[0017] The term effective amount means the amount of the formulation that will control the target pest. The effective amount will vary depending on the mixture concentration, the type of pest(s) being treated, the severity of the pest infestation, the result desired, and the life stage of the pest during treatment, among other factors. Thus, it is not always possible to specify an exact effective amount. However, an appropriate effective amount in any individual case may be determined by one of ordinary skill in the art.

[0018] In a preferred embodiment, the ratio of sabadilla alkaloids to N-octyl bicycloheptene dicarboximide is from about 1:2.5 to about 25:1, preferably from about 1:1 to about 1:10.

[0019] In another preferred embodiment, the pesticidal mixtures of the present invention may contain one or more excipients selected from the group consisting of solvents, anti-caking agents, stabilizers, defoamers, slip agents, humectants, dispersants, wetting agents, thickening agents, emulsifiers, penetrants, adjuvants, polymers, propellants and/or preservatives.

[0020] The present invention is further directed to methods of controlling a pest comprising applying a pesticidal mixture comprising an effective amount of sabadilla alkaloids and N-octyl bicycloheptene dicarboximide to the pest or the pest's environment.

[0021] In a preferred embodiment, the pest is selected from an insect and a mite.

[0022] In an embodiment, the pest controlled is selected from the group consisting of bed bugs (Hemiptera), fleas (Siphonaptera), cockroaches (Blattodea), flies (Diptera), ants (Hymenoptera), mosquitoes (Culicidae) and mites (Acari). In a preferred embodiment, the pest controlled are selected from the group consisting of common bed bugs (Cimex lectularius), house fly (Musca domestica), yellow fever mosquito (Aedes aegypti), southern house mosquito (Culex quinquefasciatus), African malaria mosquito (Anopheles gambiae), common malaria mosquito (Anopheles quadrimaculatus) and German cockroach (Blattella germanica).

[0023] The pesticidal mixtures of the present invention can be applied by any convenient means. Those skilled in the art are familiar with the modes of application including spraying, brushing, soaking, in-furrow treatments, pressurized liquids (aerosols), fogging or side-dressing.

[0024] In a preferred embodiment, sabadilla alkaloids are applied to the pest or the pest's environment at a rate from about 1 to about 1,000 grams per hectare (g/HA), preferably from about 10 to about 700 g/HA and most preferably from about 22 to about 560 g/HA.

[0025] In a preferred embodiment, N-octyl bicycloheptene dicarboximide is applied to the pest or the pest's environment at a rate from about 1 to about 10,000 g/HA, more preferably from about 10 to about 7,000 g/HA and most preferably from about 22 to about 5,600 g/HA.

[0026] In another preferred embodiment, pesticidal mixtures of the present invention comprise from about 0.05% to about 0.5% w/w sabadilla alkaloids.

[0027] In another preferred embodiment, pesticidal mixtures of the present invention comprise from about 0.05% to about 5.0% w/w N-octyl bicycloheptene dicarboximide.

[0028] As used herein, control a pest or controlling pest(s) refers to killing, incapacitating, repelling, or otherwise decreasing the negative impact of the pest on plants or animals to a level that is desirable to the grower or animal.

[0029] As used herein, pest's environment refers to any area that the pest is present during any life stage. One environment likely to be treated by the methods of the present invention includes the plants that the pest is living on and the surrounding soil. The pest's environment may also include harvested plants, gardens, fields, greenhouses, or other buildings, and various indoor surfaces and structures, such as furniture including beds, and furnishings including books, clothing, etc.

[0030] The articles a, an and the are intended to include the plural as well as the singular, unless the context clearly indicates otherwise. For example, the methods of the present invention are directed to controlling pest but this can include control of a multiple pests (such as a more than one insect or more than one insect species or more than one mite or more than one mite species).

[0031] The following examples are intended to illustrate the present invention and to teach one of ordinary skill in the art how to use the extracts of the invention. They are not intended to be limiting in any way.

EXAMPLES

Example 1German Cockroach

[0032] In this study, the response of the German cockroach (Blattella germanica) to application of a 1:1 and 1:10 ratio of sabadilla (S. officinale) alkaloids to N-octyl bicycloheptene dicarboximide will be observed. Specifically, sabadilla alkaloids and N-octyl bicycloheptene dicarboximide will be applied to the pest at the respective rates of: 1) 0.05% w/w to 0.05% w/w, 2) 0.05% w/w to 0.5% w/w, 3) 0.5% w/w to 0.5% w/w and 0.5% w/w to 5.0% w/w.

[0033] The study will be conducted by direct spray using 4 replications and 10 insects per replication. Application volume will be 0.3-1.0 ml dependent upon surface area of the target area.

[0034] The results of the study are predicted to show more than an additive effect. One can determine that the response is synergistic using the following formula: % C.sub.exp=A+B(AB/100).

[0035] % C.sub.exp=A+B(AB/100), where % C.sub.exp is the expected efficacy and in which A and B are the control levels given by the single [insecticides]. If the ratio between the experimentally observed efficacy of the mixture Cobs and the expected efficacy of the mixture is greater than 1, synergistic interactions are present in the mixture. (Gisi, Synergisitic Interaction of Fungicides in Mixtures, The American Phytopathological Society, 86:11, 1273-1279, 1996). Adopting a conservative approach, Applicant determined synergy to be present at ratios of 1.1.

Example 2House Fly

[0036] In this study, the response of the house fly (Musca domestica) to application of a 1:1 and 1:10 ratio of sabadilla (S. officinale) alkaloids to N-octyl bicycloheptene dicarboximide will be observed. Specifically, sabadilla alkaloids and N-octyl bicycloheptene dicarboximide will be applied to the pest at the respective rates of: 1) 0.05% w/w to 0.05% w/w, 2) 0.05% w/w to 0.5% w/w, 3) 0.5% w/w to 0.5% w/w and 0.5% w/w to 5.0% w/w.

[0037] The study will be conducted by direct spray using 4 replications and 10 insects per replication. Application volume will be 0.3-1.0 ml dependent upon surface area of the target area.

[0038] The results of the study are predicted to show more than an additive effect. One can determine that the response is synergistic using the following formula: % C.sub.exp=A+B(AB/100).

Example 3Common Bed Bug

[0039] In this study, the response of the common bed bug (Cimex lectularius) to application of a 1:1 and 1:10 ratio of sabadilla (S. officinale) alkaloids to N-octyl bicycloheptene dicarboximide will be observed. Specifically, sabadilla alkaloids and N-octyl bicycloheptene dicarboximide will be applied to the pest at the respective rates of: 1) 0.05% w/w to 0.05% w/w, 2) 0.05% w/w to 0.5% w/w, 3) 0.5% w/w to 0.5% w/w and 0.5% w/w to 5.0% w/w.

[0040] The study will be conducted by direct spray using 4 replications and 10 insects per replication. Application volume will be 0.3-1.0 ml dependent upon surface area of the target area.

[0041] The results of the study are predicted to show more than an additive effect. One can determine that the response is synergistic using the following formula: % C.sub.exp=A+B(AB/100).