Blue transgenic fluorescent ornamental fish

Abstract

The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Claims

1. A transgenic tetra comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra comprises the Blue tetra 1 transformation event, sperm comprising the Blue tetra 1 transformation event having been deposited as ECACC accession no. 14103001.

2. The transgenic tetra of claim 1, further defined as a fertile, transgenic tetra.

3. The transgenic tetra of claim 1, further defined as a transgenic White Tetra.

4. The transgenic tetra of claim 1, wherein the fish is homozygous for the integrated expression cassette.

5. The transgenic tetra of claim 1, wherein the fish is heterozygous for the integrated expression cassette.

6. A method of producing a transgenic tetra comprising: (a) obtaining a tetra that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra comprises the Blue tetra 1 transformation event, sperm comprising the Blue tetra 1 transformation event having been deposited as ECACC accession no. 14103001; and (b) breeding the obtained tetra with a second tetra to provide a transgenic tetra comprising the Blue tetra 1 transformation event.

7. The method of claim 6, wherein the second tetra is a non-transgenic tetra.

8. A progeny of a transgenic tetra of claim 1 that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra and progeny exhibit fluorescence and comprise the Blue tetra 1 transformation event, sperm comprising the Blue tetra 1 transformation event having been deposited as ECACC accession no. 14103001.

9. The progeny fish of claim 8, further defined as a fertile, transgenic tetra.

10. The progeny fish of claim 8, further defined as a transgenic White Tetra.

11. The progeny fish of claim 8, wherein the fish is homozygous for the integrated expression cassette.

12. The progeny fish of claim 8, wherein the fish is heterozygous for the integrated expression cassette.

13. A method of producing a transgenic fish comprising: (a) obtaining a transgenic fish in accordance with claim 8; and (b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the Blue tetra 1 transformation event.

14. The method of claim 13, wherein the second fish is a non-transgenic fish.

Description

EXAMPLES

(1) Certain embodiments of the invention are further described with reference to the following examples. These examples are intended to be merely illustrative of the invention and are not intended to limit or restrict the scope of the present invention in any way and should not be construed as providing conditions, parameters, reagents, or starting materials that must be utilized exclusively in order to practice the art of the present invention.

Example 1

Blue Transgenic Tetra

(2) Transgenic fish exhibiting a blue color are provided. The specific transgenic events embodied in these fish are designated the Blue tetra 1 transformation event. Sperm from these fish may be used to fertilize tetra eggs and thereby breed transgenic tetra that comprise these specific transgenic integration events. Sperm from this line was deposited at the European Collection of Cell Cultures (ECACC), Public Health England, CRYOSTORES, Bld. 17, Porton Down, Salisbury, SP4 OJG, United Kingdom, under the provisions of the Budapest Treaty as Blue tetra 1 (the deposit was designated as accession no. 14103001).

(3) The fluorescent transgenic fish have use as ornamental fish in the market. Stably expressing transgenic lines can be developed by breeding a transgenic individual with a wild-type fish, mutant fish, or another transgenic fish. The desired transgenic fish can be distinguished from non-transgenic fish by observing the fish in white light, sunlight, ultraviolet light, blue light, or any other useful lighting condition that allows visualization of the blue color of the transgenic fish.

(4) The fluorescent transgenic fish should also be valuable in the market for scientific research tools because they can be used for embryonic studies such as tracing cell lineage and cell migration. Additionally, these fish can be used to mark cells in genetic mosaic experiments and in fish cancer models.

(5) All of the compositions and/or methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations may be applied to the compositions and/or methods and in the steps or in the sequence of steps of the methods described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain agents that are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope, and concept of the invention as defined by the appended claims.

REFERENCES

(6) The following references, to the extent that they provide exemplary procedural or other details supplementary to those set forth herein, are specifically incorporated herein by reference. U.S. Pat. No. 7,135,613 U.S. Pat. No. 7,700,825 U.S. Pat. No. 7,834,239

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