Multifunctional formulation comprised of natural ingredients and method of preparation/manufacturing thereof

Abstract

This invention relates to a pharmaceutical preparation for the treatment of compromised tissue such as skin wounds and ulcers in humans and animals and a method of preparation. This is a multifunctional natural matrix meant for the treatment of compromised tissues which also relates to the anti-cancer transdermal patch for melanoma therapy. Further, the invention comprises for the treatment of Alzheimer's, and multiple sclerosis also. The composition consists of water-solubilized nano-sized formulation of non-aqueous solvent extract of phyto-pharmaceuticals in herbal, animal or synthetic biocompatible gel or on matrix coated or both. The composition is used as a topical device for the treatment of compromised tissues in its preferred embodiment.

Claims

1. A phytopharmaceutical gel formulation, comprising: a) a water soluble phytopharmaceutical formulation, consisting of: 0.5 to 20 percent of a centrifuged and sonicated water soluble, nano-sized, ethanol extract of the plant Emblica officinalis; 1.0 to 20 percent of a centrifuged and sonicated water soluble, nano-sized, ethanol extract of the plant Camellia sinsensis; 0.1 to 12 percent of a centrifuged and sonicated water soluble, nano-sized, ethanol extract of curcumin; and at least one dried layer having a thickness of about 1 μm to 500 μm; and b) 0.2 to 12.0 percent of Aloe vera gel.

2. The phytopharmaceutical gel formulation of claim 1, wherein said Aloe vera gel comprises 0.5 to 9.0 percent tocopherol.

3. The phytopharmaceutical gel formulation of claim 1, further comprising 0.1 to 11 percent of solubilized silver nano-particles.

4. The phytopharmaceutical gel formulation of claim 1, further comprising liposomes that encapsulate the phytopharmaceutical formulation, wherein said liposomes have a diameter of about 0.3 to 500 nanometers.

5. The phytopharmaceutical gel formulation of claim 1, further comprising a biocompatible, porous matrix carrying the at least one dried layer of phytopharmaceutical formulation.

6. The phytopharmaceutical gel formulation of claim 5, comprising a plurality of dried layers of phytopharmaceutical formulation, wherein each layer is about 10 μm to 100 μm.

7. The phytopharmaceutical gel formulation of claim 5, wherein the biocompatible, porous matrix comprises a woven cotton bandage or a silicon patch.

8. The phytopharmaceutical gel formulation of claim 5, wherein the biocompatible, porous matrix includes microneedles for transdermal drug delivery.

9. A phytopharmaceutical gel formulation, comprising: a) a water soluble phytopharmaceutical formulation, consisting of: 0.5 to 20 percent of a centrifuged and sonicated water soluble, nano-sized, ethanol extract of the plant Emblica officinalis; 1.0 to 20 percent of a centrifuged and sonicated water soluble, nano-sized, ethanol extract of the plant Camellia sinsensis; 0.1 to 12 percent of a centrifuged and sonicated water soluble, nano-sized, ethanol extract of curcumin; b) Aloe vera gel; and c) 0.1 to 11 percent of solubilized silver nano-particles, wherein the water soluble phytopharmaceutical formulation, Aloe vera gel and silver nano-particles together comprise at least one dried layer having a thickness of about 1 μm to 500 μm.

10. The phytopharmaceutical gel formulation of claim 9, comprising 0.2 to 12.0 percent of Aloe vera gel.

11. The phytopharmaceutical gel formulation of claim 10, wherein said Aloe vera gel comprises 0.5 to 9.0 percent tocopherol.

12. The phytopharmaceutical gel formulation of claim 9, further comprising liposomes that encapsulate the phytopharmaceutical formulation, Aloe vera gel, and silver nano-particles, wherein said liposomes have a diameter of about 0.3 to 500 nanometers.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 illustrates a graph displaying in vitro anti-oxidant potential of physico-chemical treated nano-sized extracts.

(2) FIG. 2 illustrates a bar graph of mean zone of inhibition of E. coli, S. bacillus, Staphylococcus aureus, Pseudomonas aeruginosa Candida albicans using formulation F1-F5 and 1× antimicrobial solution at 24 Hrs.

(3) FIG. 3 illustrates a bar graph of the LD50 average of the nano-sized water solubilized curcumin extracts on HCT-15, KB and INT-407 cancer cell lines was found up to be 4.5 μg/ml.

(4) FIG. 4 illustrates a scanning electron microscopic image of water soluble nano-curcumin prepared by physico-chemical treatment.

(5) FIG. 5 illustrates a scanning electron microscopic image of water soluble nano-extract of Emblica officinalis prepared by physico-chemical treatment.

(6) FIG. 6 illustrates a scanning electron microscopic image of water soluble nano-extract of Camellia sinensis prepared by physico-chemical treatment.

(7) FIG. 7 illustrates a light microscopic image of HCT-15 cell line at 24 Hrs without treatment with water soluble nano-curcumin prepared by physico-chemical treatment.

(8) FIG. 8 illustrates a light microscopic image of HCT-15 cell line at 24 Hrs with treatment with 3.5 μg/ml of water soluble nano-curcumin prepared by physico-chemical treatment.

DETAILED DESCRIPTION OF THE SEVERAL EMBODIMENTS

(9) It should be noted that the particular description and embodiments set forth in the specification below are merely exemplary of the wide variety and arrangement of instructions which can be employed with the present invention. The present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. All the features disclosed in this specification may be replaced by similar other or alternative features performing similar or same or equivalent purposes. Thus, unless expressly stated otherwise, they all are within the scope of present invention.

(10) Various modifications or substitutions are also possible without departing from the scope or spirit of the present invention. Therefore, it is to be understood that this specification has been described by way of the most preferred embodiments and for the purposes of illustration and not limitation.

(11) The present invention provides a formulation with enhanced bioavailability of its ingredients, working together in synergistic way. The said formulation of present invention is applied on a multi-purpose wound healing matrix with bandage.

(12) The present invention relates to a natural formulation and associated matrix bandage for compromised tissues viz, skin having wounds or melanoma. The formulation comprises of nano and water soluble formulation of water insoluble herbal extract to enhance their bioavailability.

(13) The most important part is that natural water insoluble ingredients are used as part of formulation and their bioavailability is enhanced to great extent to make them effective, in much more range/amount than already known in prior art.

(14) Since the components in the formulation are from herbal/natural resources, it is very safe and eco-friendly and does not produce any adverse effect on the skin. The herbal gel formulation of the present invention consists of a natural herbal extract of Curcuma longa, curcumin, non-aqueous preferably ethanolic extract of Emblica officinalis and Camellia sinensis and silicon and/or Aloe vera. The gel can be used directly on to the compromised tissue or combined with a non-adherent carrier of breathable porous matrix of polyurethane or cotton gauze or equivalent. The active ingredients of the gel viz. nano-formulated water solubilized agents/extracts e.g. water soluble pure nano-curcumin are also formulated as transdermal drug delivery patch for the delivery of herbal molecules for the treatment of cancer viz. melanoma. Further, the present invention also comprises beneficial use for Alzheimer's and multiple sclerosis.

(15) The present invention provides a healing wound bed matrix containing characteristics of antibiotic, anti-oxidant, anti-inflammatory, anti-cancer and biocompatible within or on the surfaces of the wound. Further, the present invention is also beneficial for Alzheimer's and multiple sclerosis treatment.

(16) The matrix bed is in the form of fabric or in the form of bandage or in the form of fiber bundles or mesh or in the form of gel or in the form of transdermal patch (e.g. silicon) or in the form of breathable film and porous matrix of polymer e.g. Polyurethane (PU). The base matrix could any one of the above mentioned which can be coated three dimensionally with bioactive nano-formulation of Aloe vera or silicone gel containing curcumin, Emblica and Camellia extract as a primary layer and which on one side is coated with layer by layer of the bioactive nano-formulation of Aloe vera or silicone gel containing curcumin, Emblica and Camellia extract having higher concentration. This multi-layer differential coating forms a graduated barrier and release layer of the formulation. The nano-formulation can be layered coated either with individual bioactive extract or in different combination of four viz. Curcuma extract, Curcumin; Emblica and Camellia. The layered coating can also be done on alternate basis of the individual or in combinations of some or all. Further the same could be altered using silicon based gel formulation. The transdermal patch based on silicone base material can also be used to deliver the bioactive extract preferably Curcuma extract, Nano-Curcumin towards dealing with cancer therapy, preferably melanoma.

(17) The invention thus provides a matrix with multifunctional wound healing and anti-cancer therapy. The natural components which are used in various compositions for medicinal values here are: Curcuma extract, curcumin, Emblica extract, Camellia extract, Aloe vera extract and/or Aloe vera gel or equivalent gel.

(18) Other compositions like silicon based gel either alone or in combination with Aloe gel and or extracts of the four may also be used.

(19) Other more composition like silicon based gel or patch either alone or in combination with Aloe gel and or extracts of the four preferably nano-curcumin alone or in combination with nano-extract of Emblica extract, Camellia extract may be used as anti-cancer patch preferably for melanoma.

(20) The embodiments of the present invention provide the matrices exhibiting anti-microbial, anti-inflammatory, anti-oxidant and anti-cancer properties. The matrix is in the form of fabric or in the form of bandage or in the form of fiber or in the form of gel or in the form of transdermal patch having micro needles. Blend of cancer (preferably melanoma) healing matrix components of wound bed matrix with nano-curcumin, with or without its other constituents. Nano-Curcumin and other nano-extract are made by physico-chemical treatment of the aqueous insoluble extracted powder (preferably ethanol extract) followed by ultrasonic wave at least 25 KHz for 15 min-5 Hrs at ice temperature to obtain gradation of the particle size and mixed aqueous solution of the some of the extracts or all together at different ratio. A method to delivering curcumin nano-particles targeting melanoma is preferably via transdermal drug delivery approaches using silicon based transdermal patch having micro needles.

(21) Accordingly, the present invention provides a ready to use, flexible, multifunctional biocompatible formulation and bandage dressing and a method of manufacturing thereof, said formulation and dressing comprising a matrix based structure, having a plurality of biopolymers or synthetic polymers in the form of a formulation, with all ingredients working together in synergy, said matrix contained with the water soluble nano-form of non-aqueous solvent-extracted, water-insoluble phyto-pharmaceuticals from different natural sources, and a gel that is from herbal, animal or synthetic sources; such that the ingredients of formulation have much enhanced bioavailability than their naturally known values thus making it highly effective for wound healing, drug-delivery, anti-cancer therapy, anti-Alzheimer's and anti-multiple sclerosis.

(22) In an embodiment, said plurality of biopolymers are selected from but not limited to Emblica officinalis, Acacia catechu, Azadirachta indica, Curcuma longa, Camellia sinsensis, Mimosa pudica, Ocimum sanctum, Phyllanthus emblica, Ricinus communis, Terminalia arjuna, Withania somnifera, Bacopa monnieri etc, combined with a gel that can be natural source like alginate, Aloe vera, carboxy methyl cellulose, gelatin, pectin or synthetic polymers like silicon/siloxane/Dimethicone/methicone/siloxysilicate and their derivatives.

(23) In another embodiment, said matrix bed is in the form of fabric or in the form of bandage or in the form of fiber bundles or mesh or in the form of gel or in the form of transdermal patch (e.g. silicon) or in the form of breathable film and porous matrix of polymer e.g. Polyurethane (PU).

(24) In another embodiment, said base matrix is coated three dimensionally, preferably with bioactive nano-formulation of Aloe vera or silicone gel containing curcumin, Emblica and Camellia extract as a primary layer and which on one side is coated with layer by layer of the bioactive nano-formulation of Aloe vera or silicone gel containing curcumin, Emblica and Camellia extract having higher concentration, such that this multi-layer differential coating forms a graduated barrier and release layer of the formulation.

(25) In yet another embodiment, said nano-formulation is layered coated either with individual bioactive extract or in different combination of four viz. Curcuma extract, Curcumin; Emblica and Camellia.

(26) In another embodiment, said layered coating is also be done on alternate basis of the individual or in combinations of some or all or altered using silicon based gel formulation.

(27) In yet another embodiment, said transdermal patch based on silicone base material is used to deliver the bioactive extracts preferably Curcuma extract, Nano-Curcumin towards dealing with cancer therapy, preferably melanoma.

(28) In another embodiment, said system comprising of a ready to use, flexible, multifunctional biocompatible matrix based dressing contained with water-soluble nano-form of non-aqueous solvent extracted water insoluble phyto-pharmaceutical from different plant sources, and gel that can be from herbal, animal or synthetic sources, for the for effective wound healing, anti-cancer therapy and the manufacturing of the same thereof.

(29) In another embodiment, said dressing is made using a suitable material, selected from either a medical grade polyurethane or equivalent polymer film or cotton in the form of woven or nonwoven gauze, foam or scaffold made from biopolymers, such that it allows air to flow through it making the dressing breathable.

(30) In yet another embodiment, said dressing consists of a layer of coating over the matrix, said coating being gel like in nature and is incorporated with the formulation of phyto-pharmaceutical, extracted from different plant sources that promotes the wound healing and regeneration of skin, said formulation having all ingredients working together in synergy and having enhanced bioavailability of naturally water-insoluble ingredients.

(31) In another embodiment, said formulation of the phytopharmaceutical comprises of water soluble extract that is prepared from two or more plant sources selected from but not limited to Emblica officinalis, Acacia catechu, Azadirachta indica, Curcuma longa, Camellia sinsensis, Mimosa pudica, Ocimum sanctum, Phyllanthus emblica, Ricinus communis, Terminalia arjuna, Withania somnifera, Bacopa monnieri etc, combined with a gel that can be natural source like alginate, Aloe vera, carboxy methyl cellulose, gelatin, pectin etc or can be synthetic polymers like silicon/siloxane/Dimethicone/methicone/siloxysilicate and their derivatives, polyvinyl alcohol, polyvinylpyrrolidone etc.

(32) In another embodiment, said formulation of the phyto-pharmaceutical comprises of water soluble extract that can be obtained by mixing different non-aqueous extracts using non-aqueous solvent from plants preferably Emblica officinalis, in the range of 0.5%-20%, Camellia sinsensis in the range of 1%-20%, and making water soluble curcumin solution in the range of 0.1%-12% mixed with Aloe vera gel in the range of 0.2%-12% along with 0.5-9% tocoferol or all mixed together, accompanied with nano-silver or nano-silver mixed with Aloe vera accompanied with Aloe-vera layer by layer.

(33) In another embodiment, a layer by layer matrix formulation is done by preparing 0.3-2.5% Aloe-vera solution in water followed by preparation of nano-crystalline silvel solution using 1-10% silver, 0.5-6 parts of 0.5-3% Aloe vera solution and 0.3-5 part of nano-silver solution and mixing the solution uniformly using stirrer at the speed of 800-1200 rpm for 10-40 mins and coating the non-adherent porous breathable matrix, preferably 3D coating, then drying the coated matrix at 25-175° C. in presence or absence of light and repeating this step 2-10 times preferably 2-3 times; preparing 2-6% Aloe vera solution in water and another solution of 2-10% Aloe vera in nano-silver solution prepared earlier; taking the dried coated gauze previously to give 10-500 μm thick coats of 2-10% Aloe vera in nano-silver solution on one side using the coating machine followed by coating with 10-500 μm 2-6% Aloe vera in water on the same or both sides and repeating the process 2-10 times with or without drying the matrix in between the coating steps; drying of the intermediate and the final coated matrix at 25-175° C. in presence or absence of light; optionally combining the steps used for nano-silver with nano-phytoextracts in the 0.2-20 ratio of both preferably 1:4 ratio or optionally replacing the steps used for nano-silver, nano-silver with other nanoparticles of other metal and/or their derivatives viz gold, zinc, zinc oxide, copper, copper oxide, etc.; also optionally in steps used for nano-silver, replacing entirely the metallic nanoparticles by nano-phyto-extracts and coating alternatively alone or along with Aloe vera gel to obtain the desired final product.

(34) In another embodiment, said non-aqueous solvent is preferably ethanol, methanol, hexane, toluene, chloroform, di-chloro-methane, methylene chloride, acetone, alone or in combination with other solvent in ratio of 1:1-0.1:5 wt/wt.

(35) In another embodiment, different mix of water-solubilized nano-formulation of non-aqueous extract have a range of 0.2-15%. In yet another embodiment, water-solubilized nano-sized phyto-pharmaceutical is further formulated in nano-carrier for targeted delivery in the form of nano-particles, liposome, polymer nano-encapsulated, sized 0.3-500 nm, linker conjugated or ligand conjugated for sustained, controlled and targeted delivery meant for anti-cancer, anti-Alzheimer's and anti-multiple sclerosis.

(36) In another embodiment, in nano-carrier for targeted delivery in the form of nanoparticle, liposome, polymer nano-encapsulated, sized 0.3-500 nm, where linker conjugated or ligand conjugated are relating to blood brain barrier Receptor, blood brain tumor brain receptor, angiogenesis Receptor; cell penetrating peptide-TAT peptide (AYGRKKRRQRRR), transferrin receptor, Nicotinic acetylcholine receptors (nAChRs), brain capillary endothelial cells receptors, integrin αvβ3-cRGD, LVD peptide; NG2 proteoglycan-TH10 peptide TAASGVRSMH, EGFR receptor, COX-2 receptor, ER+, PR+17b-beta estradiol, Used to manage tumors that test positive for either estrogen or progesterone receptors, HER2+ anti-HER2 mAb, KRRQQKIRKYTMRR, triple positive, triple negative, folate receptor and other equivalent. In another embodiment, encapsulated form of said phyto-pharmaceuticals is linker conjugated or ligand conjugated.

(37) In another embodiment, said nano-sized nano-formulation is coated onto the stent to be a part of constituent to prepare drug eluting stent.

(38) In another embodiment, the synthetic polymers like silicon/siloxane/Dimethicone/methicone/siloxysilicate and their derivatives may contain aqueous or non-aqueous solubilized silver nano-particles in the range of 0.1-11%.

(39) In another embodiment, non-aqueous solubilized nano-silver preferably is in toluene and or hexane.

(40) In another embodiment, in order to get pure nano-silver loaded silicon patch, preferably toluene solubilized nano-silver is mixed with soft skin silicon adhesive and kept for 5-25 min under flowing air preferably under vacuum at room temperature followed by curing at 60 to 90° C. for a time period of 10 min to overnight.

(41) In another embodiment, the layering of each zone of the bioactive agents may be from 1 μm-500 μm.

(42) In another embodiment, said matrix is of silicon based contains nano-phyto-extracts and/or nano-silver or both or alone as a silicon based patch may be or may not be with microneedles. The nano-phyto-extracts and nano-silver can be adsorbed, linked, conjugated or encapsulated with micro or nano-carriers.

(43) In another embodiment, said matrix is of silicon based and contains nano-phyto-extracts and/or nano-silver or both or alone as a silicon based patch, manufactured by using 0.5-15% of nano-phyto-extracts, nano-curcumin or curcumin and nano-phyto-extracts; and coating the solution on a non-adherent surface preferably polyurethane film and then keeping it at 25-120° C. preferably at 80° C. from 30 mins to overnight.

(44) In another embodiment, said formulation is accompanied by polymers like gelatin, collagen, chitosan, polyvinyl alcohol, polyvinyl pyrrhilidone, polyethylene glycol, mucopolysacchharides, carboxymethyl cellulose.

(45) In another embodiment, the preferred ethanol extracts are prepared from the leaves of Camellia sinsensis and fruit of Emblica officinalis, in the following steps: adding dried and crushed plant to 100% non-aqueous solvent e.g. ethanol solution and keeping over magnetic stirrer for 20 hrs at 60° C.; separating the ethanolic extract by centrifugation at 8000 rpm for 15 mins; allowing the supernatant to dry in rotary evaporator or in oven at 30° C.-85° C.; preparing the solution of the desired concentration by dissolving the dried ethanolic extract in a 10%-20% ethanol solution and sonication at 5-85 amplitude for 15 min to 5 hrs followed by evaporation of excess of ethanol to form water soluble plant extract.

(46) In another embodiment, said dressing is prepared by coating the phyto-pharmaceutical formulation over the woven cotton bandage followed by drying in air at room temperature in dark.

(47) In yet another embodiment, said dressing is sterilized using gamma irradiation.

(48) In another embodiment, said dressing is flexible and hence is used to cover the uneven surface of the compromised tissues of the body.

(49) In another embodiment, said dressing is used for the wound management and is also used as transdermal drug delivery system preferably of silicon patch with micro needles towards the delivery of active phyto-pharmaceutical and their carrier for the treatment of the cancer preferably melanoma.

(50) In another embodiment, said dressing possesses antimicrobial properties to prevent the growth of microorganism over the wounds thus accelerating the healing process.

(51) In another embodiment, said dressing is capable of absorbing exudates from the wounds to provide favorable condition for the wound repair.

(52) In another embodiment, said dressing can be used for various applications like exudating wounds in case of diabetic foot ulcers, venous ulcers or can be used for the covering the wound site created due to burns and also after surgical procedures.

(53) In another embodiment, said dressing is preferably prepared in the form of a non-adherent gauze or patch.

(54) The present invention is mainly for medical purpose and the broad range applications of the invention are as: Anti-microbial Anti-inflammatory Analgesic Antioxidant Wound healing Radiation poisoning Modulates immunity Biocompatible Haemostatic Non allergic Nano based technology for drug releasing Anti-cancer properties Multiple sclerosis Alzheimer's disease

(55) The following examples are for the purpose of illustration only and should not be construed to limit and the scope of the present invention.

Example 1

(56) Preparation of non-aqueous extract of Emblica officinalis, Camellia sinensis and Curcuma longa is performed preferably using 99.99% of ethanol followed by solvent evaporation to get water insoluble extract. The water insoluble extract was subjected to physico-chemical treatment by altering the pH of the solution followed by ultrasonic treatment at 5-25 amplitude for 2 to 5 hrs to get water soluble nano-sized fraction of non-aqueous (e.g. ethanolic) extracts. Yet another approach of curcumin, 1M solution of NaOH was prepared (pH of the solution was 12.5-12.6). 5-10% Curcumin solution was prepared in 1M solution of NaOH with continuous mixing. The prepared solution was then neutralized with 5N HCl and then adjusted to pH 5.5 The prepared solution was then sonicated for 5 hrs with amplitude 25 and temperature maintained below 30° C. The obtained nano-formulation was assayed for anti-microbial, anti-inflammatory, anti-oxidant and/or anti-cancer potentials.

Example 2

(57) The obtained nano-formulation in example one is/are further subjected for formulation using silicon/siloxane/Dimethicone/methicone/siloxysilicate based materials such as Dow Corning silicone elastomeric blend and soft skin adhesive etc from Dow Corning. The mixed subjects are kept for curing at temperature ranging from 10-150° C. to get gelly matrix, tacky less and tacky patches. The said nano-formulation could be loaded either individually or in combination onto the silicon based patches with micro needles meant for transdermal drug delivery. Individuality of the said nano-formulation such as water soluble pure curcumin could be loaded onto the micro needles containing silicon patch as a transdermal patch for melanoma therapy. Above obtained gel formulation may also contain tocoferol, vit E and glycerol.

Example 3

(58) The obtained nano-formulation in example one is/are coated onto the fabric preferably non-adherent fabric or polyurethane (PU) film or foam or breathable matrix. In a formulation of dressing matrix, nano-formulation of the three extract of Emblica officinalis, Camellia sinensis and Curcuma longa in the ratio of 1:1:1 at 5-15% concentration is coated on the pre-coated of silicon and/or Aloe vera gel fabric or PU film or foam or breathable matrix and dried at 4-25° C. followed by multiple coating-drying cycle layer by layer (L-B-L) to get different gradation of the bioactive agents. The layering thickness of each layered zone can be from 1 μm-500 μm. Multi-layered gradation of nano-formulated bioactive agents could be in different form such as (F1) Curcumin-Emblica officinalis-Camellia sinensis (F2) Camellia sinensis-Emblica officinalis-Curcumin (F3) Emblica officinalis-Vit E-Camellia sinensis-Curcumin (F4) Camellia sinensis-Aloe vera-Emblica officinalis-Aloe vera-Curcumin-Aloe vera (F5) Camellia sinensis-silicon-Emblica officinalis-Aloe vera-Vit E-Curcumin and so on.

Example 4

(59) Take 10-50 ml of Dow corning soft skin adhesive and add 2-9 ml of Curcumin nano-particles dissolved in Dow corning SPE solution 2011 and mixed uniformly, and then make complete mixture with using the complete blend of soft skin adhesive. Cast the solution on to PU film and keep in air circulating oven at 80° C. for 10 mins. In another approach, keep in oven at 80° C. for 30 mins and 100° C. In yet another approach, curcumin nano-particles may be replaced or combined with silver nano-particles. To get only nano-silver loaded silicon patch, toluene solubilized nano-silver is mixed with Dow corning soft skin adhesive and kept for 5-25 min under flowing air or under vacuum at room temperature followed by curing at 80° C. for 10 min to overnight.

Example 5

(60) Take 20 ml of 5% curcumin nano-particle solution add 5 ml of 5% Camellia sinsensis extract in water, and then add 5% of Emblica officinalis extract in water, into it. Mix the complete solution by sonicating the extract blend as stated in example 1. Coat the blend over the woven cotton gauze (coated with non-adherent solution) using the coating machine. Allow the coated gauze to dry in air at 25° C. In another approach, take 50 ml curcumin nano-particles solution and use the solution for coating the pre-coated gauze using the coating machine. Allow the coated gauze to dry in air at 25° C. Prior coating, all the nano-sized extracts were in 0.5% of Aloe vera reconstituted gel.

Example 6

(61) Take 15 ml of 7.5% polyvinyl alcohol solution and then add 12 ml of 10% gelatin solution followed by adding 1 ml each of 2% chitosan solution, PEG 200 and glycerol. Mix the solution to prepare a homogenous blend to which adds 5 ml each of Dow corning soft skin adhesive complete pack. Mix the solution to form a homogenous mixture and then cast the blend on to the PU films and allow it to dry in air or oven at 60° C. In another formulation, take 15 ml of 7.5% polyvinyl alcohol solution and then add 12 ml of 10% gelatin solution followed by adding 1 ml each of 2% chitosan solution, PEG 400 and glycerol. Mix the solution to prepare a homogenous blend to which add 5 ml each of Dow corning skin adhesive and mix the solution to form a homogenous mixture and to which add 2 ml of Curcumin nano-particles dissolved in Dow corning SPE solution 2011, mix them properly, and then cast the blend on to the PU films or non-adherent gauze and allow it to dry in air or oven at 60° C. In yet another formulation, take 15 ml of 7.5% polyvinyl alcohol solution and then add 12 ml of 10% gelatin solution followed by adding 1 ml each of 2% chitosan solution, PEG 200 and glycerol. Mix the solution to prepare a homogenous blend to which adds 5 ml each of Dow corning skin adhesive. Mix the solution to form a homogenous mixture and to which add 2 ml of silver nano-particles with or without nano-curcumin, mix them properly, and then cast the blend on to the PU films and allow it to dry in air or oven at 60° C.

Example 7

(62) Prepare 0.5% P15 solution in USP grade water followed by reparation of nano-crystalline silvel solution using 1-10% silver.

(63) Now take 3 parts of 0.5-3% Aloe vera solution and 1 part of nano-silver solution and mix the solution uniformly using stirrer at the speed of 800-1000 rpm for 10-20 mins and coat the non-adherent coated smart gauze and or another matrix mentioned before. The obtained coated matrix is dried at 25-175° C. avoiding exposure to the light while drying and this step is repeated. Now prepare 2-6% Aloe vera solution in water and another solution of 2-10% Aloe vera in nano-silver solution prepared earlier.

(64) Take the dried coated gauze previously to give 10-100μ thick coats of 2-10% Aloe vera in nano-silver solution on one side using the coating machine followed by coating with 10-100μ 2-6% Aloe vera in water on the same side using the coating machine and repeat the process with or without drying the matrix in between the coating steps. Drying of the intermediate and the final coated matrix takes place at 25-175° C. avoiding exposure to the light while drying. The obtained products were tested on mice for their wound healing potentials comparing with existing dressing in market of the class.

(65) Discussion of the Experiments Performed

(66) The prepared multifunctional nano-formulation have efficacy like anti-microbial, biocompatible, anti-oxidant and anti-inflammatory and anti-cancer. Various experiments were conducted to check the said efficacy of matrix. Antimicrobial activities were studies using following bacteria and fungi viz. Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus (including MRSA), Candida albicans, Aspergillus niger.

(67) The biocompatibility, anti-oxidant and anti-inflammatory property of the said matrix were evaluated using mouse L929 fibroblastic cell line and human dermal fibroblasts viability, H.sub.2O.sub.2, DPPH (2,2-diphenyl-1-picrylhydrazyl) inhibition and Cox, Lox inhibition assay, respectively. The anti-cancer efficacy was tested using MCF-7, KB, INT-407, HCT-15, Colo-205, A-431, Hela cell line and the toxicity were compare using human normal primary fibroblast (HNPF).

(68) The obtained results of anti-oxidant potential of the nano-sized extract contained formulation were considerably efficacious. The in-vitro anti-oxidant potential of some of the nano-sized extract and formulation are shown in FIG. 1. Similarly, anti-microbial activity, anti-inflammatory and anti-cancer potential were also considerably efficacious and the data for some of them are shown in this patent. Aqueous solution of nano-formulation of all three phyto-extracts in different combination at below 5% base concentration also resulted into 99.999% reduction in microbial CFU/ml in all time frames 30, 60, 90 and 120 seconds using time kill method. FIG. 2 is showing the anti-microbial potential of the formulation (F1-F5). FIG. 3 is showing the killing concentration of the water soluble nano-sized extracts. LD50 average of nano-curcumin on all used cancer cell lines was found up to be 4.5 μg/ml, which classifies the same as anti-cancer agent at very low concentration. Further, the combined formulation using nano-sized water soluble extract was found to have LD50 at concentration less than 1 μg/ml and it goes even beyond 0.2 μg/ml depending on cell types (FIG. 3). Further, dose toxicity on HNPF was very low even at very high dosage more than 30 μg/ml the novel nano-phyto-solution were non-toxic to HNPF which shows LD50 potential against the cancer cell lines while keeping normal cell almost unaffected. Anti-cancer property on some of the used cancer cell lines are shown in FIG. 3.

(69) FIG. 1: In vitro anti-oxidant potential of physico-chemical treated nano-sized extracts. Data shows the IC50 value of the extract i.e. concentration at which H.sub.2O.sub.2 is inhibited by 50%. NCu: —Nano-sized curcumin; NEo: —nano-sized extract of Emblica officinalis; NCs: —nano-sized extract of Camellia sinensis; NCu-NEo-NCs: —1:1:1 ratio of NCu, NEo and NCs.

(70) FIG. 2: Mean zone of inhibition of E. coli, S. bacillus, Staphylococcus aureus, Pseudomonas aeruginosa Candida albicans using formulation F1-F5 and 1× antimicrobial solution at 24 Hrs. F1: Curcumin-Emblica officinalis-Camellia sinensis; F2: Camellia sinensis-Emblica officinalis-Curcumin; F3: Emblica officinalis-Vit E-Camellia sinensis-Curcumin; F4: Camellia sinensis-Aloe vera-Emblica officinalis-Aloe vera-Curcumin-Aloe vera; F5: Camellia sinensis silicon-Emblica officinalis-Aloe vera-Vit E-Curcumin; 1×: 1× antimicrobial solution.

(71) FIG. 3: The LD50 average of the nano-sized water solubilized curcumin extracts on HCT-15, KB and INT-407 cancer cell lines was found up to be 4.5 μg/ml. A combined formulation using nano-sized water soluble extract was found to have LD50 at concentration less than 1 μg/ml. NCu: —Nano-sized curcumin; NEo: —nano-sized extract of Emblica officinalis; NCs: —nano-sized extract of Camellia sinensis; NCu-NEo-NCs: —1:1:1 ratio of NCu, NEo and NCs.

(72) The sizes obtained from the physico-chemical treatment of the extract were below 500 nm as shown in the scanning electron microscopic images 1, 2 and 3. And the LD50 of the nano-curcumin on HCT-15 was found to be 3.5 μg/ml. The morphology and concentration dependent killing of the HCT-15 cell lines are shown in the image 4&5 and FIG. 3 respectively.

(73) The wound healing experimentation on mice were significantly efficacious in terms of epithilialization, wound contraction, and management of inflammation and pain comparing to other groups of mice used existing dressing of the same class.

(74) FIG. 4: Scanning electron microscopic image of water soluble nano-curcumin prepared by physico-chemical treatment.

(75) FIG. 5: Scanning electron microscopic image of water soluble nano-extract of Emblica officinalis prepared by physico-chemical treatment.

(76) FIG. 6: Scanning electron microscopic image of water soluble nano-extract of Camellia sinensis prepared by physico-chemical treatment.

(77) FIG. 7: Light microscopic image of HCT-15 cell line at 24 Hrs without treatment with water soluble nano-curcumin prepared by physico-chemical treatment.

(78) FIG. 8: Light microscopic image of HCT-15 cell line at 24 Hrs with treatment with 3.5 μg/ml of water soluble nano-curcumin prepared by physico-chemical treatment.

(79) The obtained results of the potential and properties of the product of this invention were found considerably efficacious.

(80) This product has resulted into 99.999% reduction in microbial CFU/ml in all time frames 30, 60, 90 and 120 seconds using time kill method.

(81) This clearly indicates the technical advancement as compared to prior art.