Esters or salts of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone and application thereof in preparation of animal antibacterial agents and growth promoters used in feed

Abstract

Esters or salts of 2-hydroxy-4-propyleyclohepta-2,4,6-trienone, and applications thereof in preparation of animal antibacterial agents and growth promoters used in feed. The esters or salts of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone has a structural formula as shown in formula 1 or 2: in formula 1, R1 represents heptadecyl; and in formula 2, L is sodium, potassium, calcium, magnesium, zinc, copper or manganese. The esters or salts of 2-hydroxy-4-propyleyclohepta-2,4,6-trienone, as shown in formula 1 or 2, have high stability and safety, and good antibacterial and growth promotion effects, which make them preferable to be used as animal antibacterial agents and growth promoters in feed, and have a very good application prospect in the cultivation industry. ##STR00001##

Claims

1. A compound which is an ester of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone, according to the following structural formula 1: ##STR00012## wherein, R1 represents heptadecyl; or a compound which is a salt of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone, according to the following structural formula 2: ##STR00013## wherein, L is sodium, potassium, calcium, magnesium, zinc, copper or manganese.

2. The compound according to claim 1, wherein L is magnesium, zinc, copper or manganese.

3. A feed additive containing an animal antibacterial agent, comprising the compound of claim 1.

4. The feed additive according to claim 3, wherein said animal includes pigs, chickens, ducks, geese, beef cattle, dairy cattle, sheep, fish, shrimp, foxes, martens or raccoon dogs in all growth stages.

5. The feed additive according to claim 3, wherein a dosage of the compound in animal feed is 0.1200 ppm.

6. A feed additive containing a growth promoter, comprising the compound of claim 1.

7. The feed additive according to claim 6, wherein said animal includes pigs, chickens, ducks, geese, beef cattle, dairy cattle, sheep, fish, shrimp, foxes, martens or raccoon dogs in all growth stages.

8. The feed additive according to claim 6, wherein a dosage of the growth promoter in the animal feed is 0.1200 ppm.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is an HPLC chromatogram of the manganese(II) 7-oxo-3-propylcyclohepta-1,3,5-trienolate in Embodiment 8.

DETAILED DESCRIPTION OF THE EMBODIMENTS

(2) The present invention is described in further detail with reference to embodiments which shall not be regarded as limits in the present invention.

Embodiment 1

Structural Formula

(3) ##STR00004##

Preparation of 7-oxo-3-propylcyclohepta-1,3,5-trien-1-yl stearate (IST-009-050)

Preparation Process

(4) 2-hydroxy-4-propylcyclohepta-2,4,6-trienone (3.28 g, 20 mmol, 1 eq) and triethylamine (110 eq) were dissolved in 100 ml of dichloromethane; at 10 C.25 C., to the resulting solution was added dropwise slowly with a mixture of n-octadecanoyl chloride (0.85 eq) and about 30 ml of dichloromethane. The resulting reaction mixture was stirred for 3-8 h. Thin layer chromatography (TLC, developing solvent: petroleum ether:ethyl acetate=5:1), in which a new spot appeared while an impurity spot appeared at the initial spot, showed that almost all the reactants were consumed. Then the resulting reaction solution was washed with 100 ml of water and then with saturated salt water, but fluorescence was still observed at the initial spot of TLC. The organic phase was then wash with dilute aqueous solution of potassium hydroxide, mixed with 8 g of silica gel, and separated over a silica column, wherein the eluent was a mixture of petroleum ether, ethyl acetate and triethylamine in a ratio of 5:1:0.02. A pure product (7-oxo-3-propylcyclohepta-1,3,5-trien-1-yl stearate) was obtained, with a purity of 98%.

(5) 7-oxo-3-propylcyclohepta-1,3,5-trien-1-yl stearate: H (CDCl.sub.3, 500 MHz) 7.0687.102 (2H, m), 6.9827.024 (1H, m), 6.8796.899 (1H, d), 2.5932.623 (2H, m), 2.4932.523 (2H, m), 1.7311.791 (2H, m), 1.6061.681 (2H, m), 1.3891.433 (2H, m), 1.256 (26H, m), 0.9500.979 (3H, m), 0.8690.890 (3H, m).

Embodiment 2

Structural Formula

(6) ##STR00005##

Preparation of potassium 7-oxo-3-propylcyclohepta-1,3,5-trienolate

(7) Potassium hydroxide (13 eq) and 2-hydroxy-4-propylcyclohepta-2,4,6-trienone were dissolved in sequence in 150 ml of ethanol at room temperature, and the resulting solution was refluxed for 1-4 h and became clear, and was cooled to room temperature wherein no precipitate was observed. And then the solution was cooled to 4020V to give a great amount of precipitate which was collected by filtration. The filter cake was washed with cold ethanol and then subjected to rotary evaporation to give a product (potassium 7-oxo-3-propylcyclohepta-1,3,5-trienolate), a purity of which was 99% by HPLC.

Embodiment 3

Structural Formula

(8) ##STR00006##

Preparation of sodium 7-oxo-3-propylcyclohepta-1,3,5-trienolate

(9) Sodium hydroxide (15 eq) and 2-hydroxy-4-propylcyclohepta-2,4,6-trienone were dissolved in sequence in 100 ml of ethanol at room temperature. The resulting solution was reflux for 15 h to (live precipitate, cooled to room temperature, stirred for 2 h, and then filtrated. The filter cake was washed with cold ethanol, and then subjected to rotary evaporation at 40 C. to give 15.9 g of product (sodium 7-oxo-3-propylcyclohepta-1,3,5-trienolate), a purity of which was 99% by HPLC.

Embodiment 4

Structural Formula

(10) ##STR00007##

Preparation of calcium 7-oxo-3-propylcyclohepta-1,3,5-trienolate

(11) Potassium hydroxide (15 eq) was dissolved in 300 ml of water at room temperature. Then the mixture was cooled to room temperature, added with 2-hydroxy-4-propylcyclohepta-2,4,6-trienone (16.4 g, 100 mmol, 1 eq), and stirred at room temperature until complete dissolution. To the resulting solution was added 80 ml of aqueous solution of calcium chloride (0.42 eq) dropwise to give a precipitate and the solution became viscous, then the solution was stirred for 23 h at room temperature, and subjected to suction filtration. Filter cake was washed with 200 ml of water twice, subjected to vacuum drying, and then dried in an oven at 35 C. to give a product (calcium 7-oxo-3-propylcyclohepta-1,3,5-trienolate), a purity of which was 99% by HPLC.

Embodiment 5

Structural Formula

(12) ##STR00008##

Preparation of magnesium 7-oxo-3-propylcyclohepta-1,3,5-trienolate

(13) Potassium hydroxide (15 eq) was dissolved in 300 ml of water at room temperature. Then the mixture was cooled to room temperature, added with 2-hydroxy-4-propylcyclohepta-2,4,6-trienone (16.4 g, 100 mmol, 1 eq), and stirred at room temperature until complete dissolution. To the resulting solution was added 80 ml of aqueous solution of hexahydrated magnesium chloride (0.42 eq) dropwise to give a precipitate and the solution became viscous, then the solution was stirred for 23 h at room temperature, and subjected to suction filtration. Filter cake was washed with 200 ml of water twice, subjected to vacuum drying, and then dried in an oven at 35 to give a product (magnesium 7-oxo-3-propylcyclohepta-1,3,5-trienolate), a purity of which was 99% by HPLC.

Embodiment 6

Structural Formula

(14) ##STR00009##

Preparation of zinc 7-oxo-3-propylcyclohepta-1,3,5-trienolate

(15) Potassium hydroxide (15 eq) was dissolved in 300 ml of water at room temperature. Then the mixture was cooled to room temperature, added with 2-hydroxy-4-propylcyclohepta-2,4,6-trienone (16.4 g, 100 mmol, 1 eq), and stirred at room temperature until complete dissolution. To the resulting solution was added 100 ml of aqueous solution of zinc sulfate (0.42 eq) dropwise to give a precipitate and the solution became viscous, then the solution was stirred for 23 h at room temperature, and subjected to suction filtration. Filter cake was washed with 200 ml of water twice, subjected to vacuum drying, and then dried in an oven at 35 C. to give a product (zinc 7-oxo-3-propylcyclohepta-1,3,5-trienolate), a purity of which was 99% by HPLC.

Embodiment 7

Structural Formula

(16) ##STR00010##

Preparation of copper 7-oxo-3-propylcyclohepta-1,3,5-trienolate

(17) Potassium hydroxide (15 eq) was dissolved in 300 ml of water at room temperature. Then the mixture was cooled to room temperature, added with 2-hydroxy-4-propylcyclohepta-2,4,6-trienone (16.4 g, 100 mmol, 1 eq), and stirred at room temperature until complete dissolution. To the resulting solution was added 100 ml of aqueous solution of copper chloride (0.42 eq), dropwise to give a precipitate and the solution became viscous, then the solution was stirred for 23 h at room temperature, and subjected to suction filtration. Filter cake was washed with 200 ml of water twice, subjected to vacuum drying, and then dried in an oven at 35 C. to give a product (copper 7-oxo-3-propylcyclohepta-1,3,5-trienolate), a purity of which was 99% by HPLC.

Embodiment 8

Structural Formula

(18) ##STR00011##

Preparation of manganese 7-oxo-3-propylcyclohepta-1,3,5-trienolate

(19) Potassium hydroxide (15 eq) was dissolved in 300 ml of water at room temperature. Then the mixture was cooled to room temperature, added with 2-hydroxy-4-propylcyclohepta-2,4,6-trienone (16.4 g, 100 mmol, 1 eq), and stirred at room temperature until complete dissolution. To the resulting solution was added 100 ml of aqueous solution of manganese sulfate (0.42 eq) dropwise to give a precipitate and the solution became viscous, then the solution was stirred for 23 h at room temperature, and subjected to suction filtration. Filter cake was washed with 200 ml of water twice, subjected to vacuum drying, and then dried in an oven at 35 C. to give a product (manganese 7-oxo-3-propylcyclohepta-1,3,5-trienolate), a purity of which was 99% by HPLC.

Embodiment 9: Thermal Stability Test of 7-oxo-3-propylcyclohepta-1,3,5-trien-1-yl Stearate in High Temperature

(20) The prepared 7-oxo-3-propylcyclohepta-1,3,5-trien-1-yl stearate (IST-009-050) was mixed with corncob powder to obtain a premix with a mass fraction of 2%. Samples (the premix) were placed in crucibles and spread, and then placed in oven at 60 C. or eat for different durations. Then 1.0 g of each of the samples (three duplicate samples from each sample) and 50 ml of ethanol were added into a conical flask, subjected to sonication for 10 min, and then filtrated through filter paper. The filtrate was transferred into a 50 ml volumetric flask, topped up to the final volume with ethanol, and then filtered with a 0.45 m microporous membrane and subjected to HPLC analysis.

(21) Chromatographic Parameters

(22) Column: Wondasil C.sub.18 (250 mm4.6 mm, 5 m);

(23) Mobile Phase: acetonitrile (100%);

(24) Detection Wavelength: 220 nm;

(25) Column Temperature: 25 C.;

(26) Injection Volume: 20 L;

(27) Flow Rate: 0.8 or 1.0 ml/min.

(28) Results of the stability test were as shown in table 1.

(29) Results showed that the thermal stability of 7-oxo-3-propylcyclohepta-1,3,5-trien-1-yl stearate is better than the unesterified 2-hydroxy-4-propylcyclohepta-2,4,6-trienone (IST-009-001), but not satisfactory yet.

(30) TABLE-US-00001 TABLE 1 Results of thermal stability test of 7-oxo- 3-propylcyclohepta-1,3,5-trien-1-yl stearate Compound Temperature ( C.) Time (h) Content (%) IST-009-050 2.0 IST-009-050 60 22 0.84 IST-009-050 80 22 0.61 IST-009-050 60 44 0.35 IST-009-050 80 44 0.03 IST-009-050 60 68 0.14 IST-009-050 80 68 0 IST-009-001 80 22 0 IST-009-001 80 44 0 IST-009-001 80 68 0

Embodiment 10: Thermal Stability Test of Different Metal Salts of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone

(31) Each of the metal salts of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone prepared in Embodiments 2-6 was mixed with corncob powder to obtain a premix with a mass fraction of 2%. Samples (the premix) were placed in crucibles and spread, and then placed in oven at 100 C. for different durations. Then 1.0 g of each of the samples (three duplicate samples from each sample) and 50 ml of ethanol were added into a conical flask, subjected to sonication for 10 min, and then filtrated through filter paper. The filtrate was transferred into a 50 ml volumetric flask, topped up to the final volume with ethanol, and then filtered with a 0.45 m microporous membrane and subjected to HPLC analysis. Results of the thermal stability test were as shown in table 2.

(32) Chromatographic Parameters

(33) Column: Wondasil C.sub.18 (250 mm4.6 mm, 5 m);

(34) Mobile phase: methanol/40 mM ammonium acetate (65:35, v/v; pH 4):

(35) Detection Wavelength: 220 nm;

(36) Column Temperature: 25 C.;

(37) Injection Volume: 20 L;

(38) Flow Rate: 0.8 or 1.0 ml/min.

(39) Results showed that the thermal stability of the metal salts of IST-009-001 were all significantly enhanced in comparison with IST-009-001, wherein the magnesium, copper and manganese salts have the highest thermal stability.

(40) TABLE-US-00002 TABLE 2 Results of thermal stability test of different metal salts of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone Residual Residual Initial content at content at content 100 C. for 100 C. for Compound (%) 20 h (%) 40 h (%) Sodium 7-oxo-3- 2.0 1.31 1.11 propylcyclohepta-1,3,5-trienolate Potassium 7-oxo-3- 2.0 1.43 1.13 propylcyclohepta-1,3,5-trienolate Calcium 7-oxo-3- 2.0 1.73 1.67 propylcyclohepta-1,3,5-trienolate Magnesium 7-oxo-3- 2.0 1.99 1.99 propylcyclohepta-1,3,5-trienolate Zinc 7-oxo-3- 2.0 1.85 1.83 propylcyclohepta-1,3,5-trienolate 2-hydroxy-4-propylcyclohepta- 2.0 0 0 2,4,6-trienone Copper 7-oxo-3- 2.0 1.99 1.98 propylcyclohepta-1,3,5-trienolate Manganese 7-oxo-3- 2.0 1.99 1.98 propylcyclohepta-1,3,5-trienolate

Embodiment 11: Optical Stability Test of Different Metal Salts of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone

(41) Each of the metal salts of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone prepared in Embodiments 2-6 was mixed with corncob powder to obtain a premix with a mass fraction of 2%. Samples (the premixes) were spread in a stability test chamber and subjected to illumination at 4500-5000 lux for 5 days. Then 1.0 g of each of the samples (three duplicate samples from each sample) and 50 ml of ethanol were added into a conical flask, subjected to sonication for 10 min, and then filtrated through filter paper. The filtrate was transferred into a 50 ml volumetric flask, topped up to the final volume with ethanol, and then filtered with a 0.45 m microporous membrane and subjected to HPLC analysis.

(42) Column: WondasilC.sub.18 (250 mm4.6 mm, 5 m);

(43) Mobile Phase: methanol/40 mM ammonium acetate (65:35, v/v; pH 4):

(44) Detection Wavelength: 220 nm;

(45) Column Temperature: 25 C.;

(46) Injection Volume: 20 L;

(47) Flow Rate: 0.8 or 1.0 ml/min.

(48) Results of the stability test were as shown in table 3. The results showed that the optical stability of the salts was enhanced, wherein results of the manganese, copper, zinc and magnesium salts were most significant.

(49) TABLE-US-00003 TABLE 3 Optical stability of different metal salts of 2-hydroxy-4-propylcyclohepta-2,4,6-trienone Residual Initial content of content 5 days later Compound (%) (%) Sodium 2.0 0.05 7-oxo-3-propylcyclohepta-1,3,5-trienolate Potassium 2.0 0.17 7-oxo-3-propylcyclohepta-1,3,5-trienolate Calcium 2.0 0.89 7-oxo-3-propylcyclohepta-1,3,5-trienolate Magnesium 2.0 1.52 7-oxo-3-propylcyclohepta-1,3,5-trienolate Zinc 2.0 1.77 7-oxo-3-propylcyclohepta-1,3,5-trienolate IST-009-001 2.0 0 (2-hydroxy-4-propylcyclohepta-2,4,6-trienone) Copper 2.0 1.9 7-oxo-3-propylcyclohepta-1,3,5-trienolate Manganese 2.0 1.96 7-oxo-3-propylcyclohepta-1,3,5-trienolate

Embodiment 12: Applications of Magnesium 7-oxo-3-propylcyclohepta-1,3,5-trienolate in Broiler Feeds

(50) 500 1-day-aged, healthy, fast-grown yellow feather broilers (female) in the same growing state and similar in weight were randomly divided into five groups according to table 4, 100 broilers in each group. Broilers of each group were fed with different dosages of magnesium 7-oxo-3-propylcyclohepta-1,3,5-trienolate or 2-hydroxy-4-propylcyclohepta-2,4,6-trienone. The broilers were kept in cages and fed with food and water ad libitum during a 30 days test period, wherein weight gain and feed conversion efficiency of the broilers fed with magnesium 7-oxo-3-propylcyclohepta-1,3,5-trienolate had been significantly improved.

(51) TABLE-US-00004 TABLE 4 Grouping of tested animals, and dosage of additives Quantity of the Dosage Administra- Group broilers Additives (ppm) tion 1 100 Blank Control Mixed with feed 2 100 Magnesium 2.5 Mixed with 7-oxo-3-propylcyclohepta- feed 1,3,5-trienolate 3 100 Magnesium 5.0 Mixed with 7-oxo-3-propylcyclohepta- feed 1,3,5-trienolate 4 100 2-hydroxy-4-propylcyclo- 2.5 Mixed with hepta-2,4,6-trienone feed 5 100 2-hydroxy-4-propylcyclo- 5.0 Mixed with hepta-2,4,6-trienone feed

(52) TABLE-US-00005 TABLE 5 Application effects of magnesium 7-oxo-3-propylcyclohepta- 1,3,5-trienolate in broiler feeds Average Average Initial weight Consump- daily Feed weight gain tion weight gain conversion Group (g) (g) (kg) (g) efficiency 1 41.03 735 151.78 24.5 2.065 2 41.24 785 155.94 26.2 1.984 3 42.47 798 151.54 26.6 1.899 4 42.62 783 156.66 26.1 1.988 5 41.18 804 153.24 26.8 1.906