LIPID FORMULATIONS FOR THE PREPARATION OF HEALTH INGREDIENT-CONTAINING HOT DRINKS

Abstract

The invention relates to mixtures comprising one or more phospholipids, wherein at least 2 wt % of the phospholipids bears a net negative charge, and/or is deprotonable, whereby the mixture comprises a weak basic salt; one or more oils, butters and/or triglycerides; one or more lipophilic or poorly water-soluble biologically active ingredients; and one or more liquid or solid polyols, sugars, monohydric and/or poly hydric alcohols. The invention further relates to sealed and air- or oxygen-impermeable and liquid-im-permeable containers comprising such mixture and the preparation thereof.

Claims

1. A sealed and air- or oxygen-impermeable and liquid-impermeable container, which encapsulates a mixture comprising: one or more phospholipids, wherein at least 2 wt % of the phospholipids: bears a net negative charge, and/or is deprotonable, whereby the mixture comprises a weak basic salt, one or more oils and/or triglycerides, one or more lipophilic or poorly water-soluble biologically active ingredients, and one or more liquid or solid polyols, polyol sugars, natural sugars, monohydric and/or polyhydric alcohols.

2. The container according to claim 1, wherein the container is a cartridge, an airtight sachet, bag, jar or an airless fluid dispenser.

3. The container according to claim 1, wherein all components of said enclosed mixtures are natural or nature-derived components and wherein the biologically active ingredients are natural and/or synthetic.

4. The container according to claim 1, wherein said one or more natural phospholipids are selected from the group consisting of soy, sunflower, linseed, rapeseed, egg, cow milk, krill lecithin and phospholipids and combinations thereof and wherein said one or more natural phospholipids have a purity grade in terms of phosphatidylcholine content of below 50%.

5. The container according to claim 1, wherein said negatively charged or deprotonable phospholipids are selected from the group consisting of phosphatidic acid, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol and combinations thereof and/or their respective salts.

6. The container according to claim 1, wherein said mixture further comprises one or more weak basic salts.

7. The container according to claim 6, wherein said one or more salts are selected from the group consisting of a bicarbonate salt, a dihydrogen and monohydrogen phosphate salt and combinations thereof.

8. The container according to claim 1, wherein said one or more liquid monohydric and/or polyhydric alcohols are selected from the group consisting of glycerol, propylene glycol, sorbitol, erythritol, tagatose, xylitol, ethanol, poly(ethylene glycol) and combinations thereof.

9. The container according to claim 8, wherein said one or more liquid nontoxic monohydric and/or polyhydric alcohols comprises glycerol.

10. The container according to claim 1, wherein the biologically active ingredient is a lipophilic or poorly water-soluble biologically active ingredient.

11. The container according to claim 1, wherein the biologically active ingredient is a hydrophilic biologically active ingredient.

12. The container according to claim 1, wherein the mixture further comprises one or more additional surfactants of natural origin or nature-derived, besides the phospholipids

13. The container according to claim 1, wherein said one or more additional surfactants are selected from the group consisting of ascorbyl palmitate, lysophosphatidyl choline and combinations thereof.

14. The container according to claim 1, wherein the mixture comprises: 5-40 wt % of a phospholipid component comprising phosphatidyl choline and negatively charged or deprotonable phospholipids selected from the group consisting of phosphatidic acid, phosphatidyl ethanolamine, phosphatidyl glycerol, a glycophospholipid, and combinations thereof, wherein said negatively charged phospholipids forms at least 2 wt % of said phospholipid component, 20-50 wt % of a mixture of glycerol and liquid or solid polyols, polyol sugars or natural sugars 5-40 wt % of a lipophilic component selected from the group consisting of natural triglyceride oils one or more natural plant bioactive substances.

15. A mixture comprising: one or more phospholipids, wherein at least 2 wt % of the phospholipids: bears a net negative charge, and/or is deprotonable, whereby the mixture comprises a weak basic salt, one or more oils, butters and/or triglycerides, one or more lipophilic or poorly water-soluble biologically active ingredients, and one or more liquid or solid polyols, polyol sugars, natural sugars, monohydric and/or polyhydric alcohols.

16. The mixture according to claim 15, which is a mixture as defined in claim 14.

17. The mixture according to claim 15 comprising: lecithin or a mixture of more natural phospholipids in an amount equal to between 5 and 40 percent of the mixture on a weight basis natural triglyceride oil or butter in an amount equal to between 5 and 40 percent of the mixture on a weight basis; a mixture of one or more liquid or solid polyols, polyol sugars, natural sugars, monohydric and/or polyhydric alcohols in an amount equal to between 10 and 75 percent of the emulsion on a weight basis; of which the lecithin/phospholipid fraction contains at least 2 weight % phospholipids bearing a net negative charge or which can be deprotonated or further deprotonated after addition of weak basic salt to the mixture.

18. A method for preparing the air- or oxygen-impermeable and liquid-impermeable container according to claim 1 comprising: a) mixing one or more phospholipids, wherein at least 2 weight % of the phospholipids is deprotonable and the other lipophilic ingredients with the one or more oils, butters and/or triglycerides, b) adding the one or more liquid or solid polyols, polyol sugars, natural sugars, monohydric and/or polyhydric alcohols with a weak basic salt dissolved in it to the mixture obtained in step a), whereby two phases are formed, c) mixing both phases under moderate heating thereby creating and ensuring a sufficiently deprotonated phospholipid fraction, d) optionally adding other biologically active ingredients that cannot be dissolved in the lipid mixture or the polyol phase beforehand to the mixture obtained in step c), e) optionally adding and mixing salts, taste enhancers and other food ingredients, such as milk powder, into the mixture obtained in step d) under moderate heating, preferably not exceeding 80? C., and f) filling an air- or oxygen-impermeable and liquid-impermeable container with the mixture obtained in step e).

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0093] FIG. 1: Photographs of beverages prepared in Example 1A (left) and Example 1B (right).

EXAMPLES

Example 1A

Preparation of a Mixture that Fails to Produce a Fine Colloidal Phospholipid Dispersion Upon Mixing with Hot Water

[0094] This experiment shows that production of a homogeneous and fine phospholipid dispersion is not possible with a premix based on phospholipids that have not been treated with weakly basic salts during preparation of the premix to produce sufficient phospholipids bearing a net negative charge and to which no additional net negatively charged lipids were added.

[0095] The following container with mixture was prepared: [0096] Aluminum foil sachet [0097] With a mixture of [0098] 2.0 gram soy phosphatidyl choline P30 (Lipoid GmbH, Germany) [0099] 1.0 gram MCT oil (De Notenshop, Netherlands) [0100] 0.3 gram tocopherol (Nutrilo GmbH, Germany) [0101] 0.2 gram ascorbyl palmitate (Dr Behr GmbH, Germany) [0102] 2.0 gram glycerol (De Tuinen BV, Netherlands)

[0103] All ingredients except for glycerol were weighed, heated and mixed at about 80 degrees Celsius until fully dissolved. Onto the resulting mixture heated glycerol was carefully poured after which both phases were mixed thoroughly, placed in the container and cooled. The container was then emptied in 100 mL of water of 90 degrees Celsius and mixed, directly, after 30 seconds and after 5 minutes. However, after preparation the resulting lipid dispersion very quickly flocculated, formed white inhomogeneities and precipitates in the whole volume of the formulation (FIG. 1).

Example 1B

Preparation of the Same Mixture as in Example 1A but then with Sodium Bicarbonate to Deprotonate the Fraction of Deprotonable Phospholipids in the Mixture

[0104] The following container with mixture was prepared: [0105] Aluminum foil sachet [0106] With a mixture of [0107] 2.0 gram soy phosphatidyl choline P30 (Lipoid GmbH, Germany) [0108] 1.0 gram MCT oil (De Notenshop, Netherlands) [0109] 0.3 gram tocopherol (Nutrilo GmbH, Germany) [0110] 0.2 gram ascorbyl palmitate (Dr Behr GmbH, Germany) [0111] 2.0 gram glycerol (De Tuinen BV, Netherlands) containing: [0112] 60 mg of sodium bicarbonate (Carl Roth GmbH, Germany)

[0113] All ingredients except for glycerol and sodium bicarbonate were weighed, heated and mixed at about 80 degrees Celsius until fully dissolved. In 10 gram of glycerol 0.4 gram of sodium bicarbonate was dissolved by heating glycerol to 120 degrees Celsius and under stirring. Onto the lipid mixture 1.5 grams of the bicarbonate-containing glycerol was carefully poured after which both phases were mixed thoroughly. Upon mixing and heating foam formed that was allowed to dissipate. The resulting mixture was placed in the container and further cooled. The container was then emptied in 100 ml of water of 90 degrees Celsius and mixed. After 1 minute of intermittent stirring a fine dispersion started to form that took another 3 minutes of waiting and stirring to be completely homogeneous (FIG. 1).

Example 2

A Sachet Containing a Preparation of the Natural Active Ingredient Palmitoylethanolamide (PEA) Based on the Technology as Described in this Patent

[0114] The following container with mixture was prepared: [0115] Aluminum foil sachet [0116] With a mixture of [0117] 1 gram soy phosphatidyl choline P30 (Lipoid GmbH, Germany) [0118] 0.8 gram MCT oil (De Notenshop BV, Netherlands) [0119] 0.2 gram cocoa butter (De Notenshop BV, Netherlands) [0120] 0.6 gram palmitoylethanolamide (Ergomax BV, Netherlands) [0121] 0.3 gram tocopherol ((Nutrilo GmbH, Germany) [0122] 0.1 gram ascorbyl palmitate (Dr Behr GmbH, Germany) [0123] 0.1 gram citrem (TER Ingredients GmbH&Co) [0124] 2.6 gram glycerol (De Tuinen BV, Netherlands) containing: [0125] 90 mg of sodium bicarbonate (Carl Roth GmbH, Germany) [0126] 0.5 g of ginger liquid flavor enhancer (Pomona BV, Netherlands)

[0127] All ingredients except for glycerol and sodium bicarbonate were weighed, heated and mixed at about 90 degrees Celsius until fully dissolved. In 10 gram of glycerol 0.4 gram of sodium bicarbonate was dissolved by heating glycerol to 120 degrees Celsius and under stirring. Onto the lipid mixture 1.5 grams the bicarbonate-containing glycerol was carefully poured after which both phases were mixed thoroughly. Upon mixing and heating foam formed that was allowed to dissipate. The resulting mixture was placed in the container and further cooled. The container was then emptied in 100 mL of water of 90 degrees Celsius and mixed thoroughly. After 1 minute of intermittent stirring a fine dispersion started to form that took another 3 minutes of waiting and stirring to be completely homogeneous. The mixture remained stable for more than one hour.

Example 3

A Sachet Containing a Preparation of Cocoa Flavanols and DHA-Rich Algal Oil Based on the Technology as Described in this Patent

[0128] The following container with mixture was prepared: [0129] Aluminum foil sachet [0130] With a mixture of [0131] 1.5 gram soy phosphatidyl choline P30 (Lipoid GmbH, Germany) [0132] 0.5 gram DHA-rich algal oil (Progress Biotech BV, Netherlands) [0133] 1.0 gram cocoa butter (De Notenshop BV, Netherlands) [0134] 0.3 gram tocopherol (Nutrilo GmbH, Germany) [0135] 0.2 gram ascorbyl palmitate (Dr Behr GmbH, Germany) [0136] 1.5 gram glycerol (De Tuinen BV, Netherlands) containing: [0137] 80 mg of sodium bicarbonate (Carl Roth GmbH, Germany) [0138] 1.5 gram erythritol (Hhoya BV, Netherlands) [0139] 50 mg xanthan gum (De Notenshop BV, Netherlands) [0140] 0.2 gram cocoa flavanols (NanJing Manhay Medical Technology Co Ltd, China) [0141] 0.5 gram dry milk powder (Campina BV, Netherlands)

[0142] All fats and oils were mixed with soy P30 and ascorbyl palmitate to form a fluid mixture under heating to 80 degrees Celsius. In 10 gram of glycerol 0.8 gram of sodium bicarbonate was dissolved by heating glycerol to 120 degrees celius and under stirring. After dissolution 10 grams of erythritol were added and melted-mixed into the glycerol phase until fully transparent. Onto the lipid mixture and under heating to 80 degrees Celsius 3 grams of the bicarbonate-containing glycerol-erythritol phase was carefully poured after which both phases were mixed thoroughly. Upon further mixing and heating foam formed that was allowed to dissipate. To this mixture the other ingredients were added (cocoa flavanols, cocoa powder, xanthan gum) and this mixture was heated again to 80 degrees Celsius and mixed thoroughly. The resulting mixture was placed in the container and further cooled. The container was then emptied in 100 ml of water of 90 degrees Celsius and mixed firmly. After 1 minute of intermittent stirring a fine dispersion started to form that took another 5 minutes of waiting and stirring to become completely homogeneous. The mixture remained stable for more than one hour.

Example 4

Capsule Containing Pomegranate Peel Extract and Pomegranate Seed Oil Based on the Technology as Described in this Patent

[0143] The following container with mixture was prepared: [0144] Nespresso aluminum coffee cartridge 10 mL (iCafilas) [0145] With a mixture of [0146] 1.0 gram soy phosphatidyl choline P30 (Lipoid GmbH, Germany) [0147] 0.8 gram MCT oil (coconut oil C8-C10 fraction) (De Notenshop BV, Netherlands) [0148] 0.25 gram pomegranate seed oil (Natural Heroes BV, Netherlands) [0149] 0.27 gram tocopherol (Nutrilo GmbH, Germany) [0150] 0.2 gram ascorbyl palmitate (Dr Behr GmbH, Germany) [0151] 0.2 gram lysophosphatidylcholine (Lipoid GmbH, Germany) [0152] 2.5 gram glycerol (De Tuinen BV, Netherlands) containing: [0153] 40 mg of sodium bicarbonate (Carl Roth GmbH, Germany) [0154] 0.5 gram propylene glycol (Sanco Industries Inc, USA) [0155] 0.2 gram pomegranate peel extract powder (NanJing Manhay Medical Technology Co Ltd, China)

[0156] All fats and oils were mixed with soy P30, lysophosphatidylcholine, ascorbyl palmitate and propylene glycol to form a fluid mixture under heating to 80 degrees Celsius. In 10 gram of glycerol 0.4 gram of sodium bicarbonate was dissolved by heating glycerol to 120 degrees Celsius and under stirring. Onto the lipid mixture and under heating to 80 degrees Celsius 1.5 grams of the bicarbonate-containing glycerol phase was carefully poured after which both phases were mixed thoroughly. Upon further mixing and heating foam formed that was allowed to dissipate. To this mixture the pomegranate peel extract was added and this mixture was heated again to 80 degrees Celsius and mixed thoroughly. The resulting mixture was poured into the aluminum coffee cartridge that was then sealed with aluminum foil in a special sealer device.

[0157] The cartridge with the mixture was then placed into a Nespresso coffee extractor machine and extracted with 100 mL warm water. The resulting phospholipid dispersion initially produced a white foam layer but quickly settled with a light pink color. It remained stable as a fine and clear dispersion for one hour.

Example 5

Capsule Containing Quercetin, MCT Oil and a Vitamin Mix Based on the Technology as Described in this Patent

[0158] The following container with mixture was prepared: [0159] Nespresso aluminum coffee cartridge 10 mL (iCafilas) [0160] With a mixture of [0161] 1.0 gram soy phosphatidyl choline P30 (Lipoid GmbH, Germany) [0162] 1.0 gram MCT oil (coconut oil C8-C10 fraction) (De Notenshop BV, Netherlands) [0163] 0.27 gram tocopherol (Nutrilo GmbH, Germany) [0164] 0.2 gram lysophosphatidylcholine (Lipoid GmbH, Germany) [0165] 2.5 gram glycerol (De Tuinen BV, Netherlands) containing: [0166] 61 mg of sodium bicarbonate (Carl Roth GmbH, Germany) [0167] 0.5 gram propylene glycol with tangerine flavor (Pomona BV, Netherlands) [0168] 0.2 gram quercetin (NanJing Manhay Medical Technology Co Ltd, China) [0169] 0.26 gram sodium ascorbate (Van Beekum BV, Netherlands) [0170] 0.16 gram zinc ascorbate (Parchem Inc, USA)

[0171] All fats and oils were mixed with soy P30, lysophosphatidylcholine, ascorbyl palmitate and propylene glycol to form a fluid mixture under heating to 80 degrees Celsius. In 10 gram of glycerol 0.25 gram of sodium bicarbonate was dissolved by heating glycerol to 120 degrees Celsius and under stirring. Onto the lipid mixture and under heating to 80 degrees Celsius 2.5 grams of the bicarbonate-containing glycerol phase was carefully poured after which both phases were mixed thoroughly. Upon further mixing and heating foam formed that was allowed to dissipate. To this mixture the quercetin and ascorbyl salts were added and this mixture was heated again to 80 degrees Celsius and mixed thoroughly. The resulting mixture was poured into the aluminum coffee cartridge that was then sealed with aluminum foil in a special sealer device.

[0172] The cartridge with the mixture was then placed into a Nespresso coffee extractor machine and extracted with 100 mL warm water. The resulting phospholipid dispersion was homogeneous and light yellow. It remained stable as a fine and clear dispersion for several hours.

Example 6

An Airtight Glass Jar Containing 10 Daily Doses of a Preparation of Cocoa Flavanols and DHA-Rich Algal Oil Based on the Technology as Described in this Patent

[0173] The following container with mixture was prepared: [0174] Airtight closed glass jar of 100 mL volume [0175] With a mixture of [0176] 15 gram soy phosphatidyl choline P30 (Lipoid GmbH, Germany) [0177] 5 gram DHA-rich algal oil (Progress Biotech BV, Netherlands) [0178] 2 gram cocoa butter (De Notenshop BV, Netherlands) [0179] 3 gram MCT (fractionated coconut oil) (De Notenshop BV, Netherlands) [0180] 1.5 gram tocopherol (Nutrilo GmbH, Germany) [0181] 0.6 gram ascorbyl palmitate (Dr Behr GmbH, Germany) [0182] 0.35 gram citrem (TER Ingredients GmbH&Co) [0183] 22.5 gram glycerol (De Tuinen BV, Netherlands) containing: [0184] 540 mg of sodium bicarbonate (Carl Roth GmbH, Germany) [0185] 10 gram erythritol (Hhoya BV, Netherlands) [0186] 500 mg xanthan gum (De Notenshop BV, Netherlands) [0187] 2 gram cocoa flavanols (NanJing Manhay Medical Technology Co Ltd, China) [0188] 2 gram cocoa powder (De Notenshop BV, Netherlands) [0189] 800 mg sodium chloride (De Notenshop BV, Netherlands) [0190] 100 mg stevia extract (Hhoya BV, Netherlands)

[0191] All fats and oils apart from DHA-rich algal oil were mixed with soy P30 and ascorbyl palmitate to form a fluid mixture under heating to 80 degrees Celsius. In 10 gram of glycerol 0.8 gram of sodium bicarbonate was dissolved by heating glycerol to 120 degrees Celsius and under stirring. After dissolution 10 grams of erythritol were added and melted-mixed into the glycerol phase until fully transparent. Onto the lipid mixture and under heating to 80 degrees Celsius 3 grams of the bicarbonate-containing glycerol-erythritol phase was carefully poured after which both phases were mixed thoroughly. Upon further mixing and heating foam formed that was allowed to dissipate. To this mixture the other ingredients were added (cocoa flavanols, cocoa powder, xanthan gum) and this mixture was heated again to 80 degrees Celsius and mixed thoroughly. Upon further cooling to 50 degrees Celsius the DHA-rich algal oil was added to the mixture and thoroughly stirred. The resulting mixture was placed in the glass jar and further cooled. Upon taking one daily dose of around 7 grams and mixing this with 100 ml of water of 90 degrees Celsius followed by a few minutes of intermittent stirring a fine dispersion started to form that took another 5 minutes of waiting and stirring to become completely homogeneous. The mixture remained stable for more than one hour.