Preparation method of green, biodegradable, and multifunctional collagen-based nanocomposite film

Abstract

Disclosed a preparation method of a green, biodegradable, and multifunctional collagen-based nanocomposite film, and overcomes the problems of difficult biodegradation, poor barrier property, and single function of food packaging materials in the existing technologies. The present invention includes the following steps: adding silicate nanosheet into deionized water for ultrasonic dispersion; then adding polyphenolic acid into the mixture, wherein a mass ratio of the polyphenolic acid to the silicate nanosheet is 1:(0.2˜1); and adjusting the pH value to 3.0˜4.0 to obtain a solution A; adding collagen with a concentration of 5 g/L into an acetic acid solution, and fully dissolving the collagen to obtain a solution B; isovolumetrically mixing the solution A with the solution B, stirring at room temperature, and adjusting the pH value to 4.5˜5.5 to obtain a casting solution; and pouring the casting solution into a polytetrafluoroethylene mold, and naturally drying to obtain a nanocomposite film.

Claims

1. A preparation method of a green, biodegradable, and multifunctional collagen-based nanocomposite film, comprising the following steps: (1) adding silicate nanosheet into deionized water for ultrasonic dispersion to form a mixture, then adding polyphenolic acid into the mixture, wherein a mass ratio of the polyphenolic acid to the silicate nanosheet is 1:(0.2-1), and adjusting pH to 3.0-4.0 to obtain a solution A; (2) adding collagen with a concentration of 5 g/L into an acetic acid solution, and fully dissolving the collagen to obtain a solution B; (3) isovolumetrically mixing the solution A with the solution B, stirring at room temperature, and adjusting pH value to 4.5-5.5 to obtain a casting solution; (4) pouring the casting solution into a polytetrafluoroethylene mold, and naturally drying to obtain a nanocomposite film; the silicate nanosheet is any one of Laponite, montmorillonite, and kaolin; and the polyphenolic acid is any one of tannic acid, folic acid, and gallic acid.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is a diagram of a collagen-based composite film, wherein a control sample is a pure collagen film;

(2) FIG. 2 is a diagram showing test results of the transmittance of the collagen-based composite film;

(3) FIG. 3 is a diagram showing the results of the antioxidant property of a tannic acid-Laponite-collagen nanocomposite film.

DETAILED DESCRIPTION OF THE PRESENT INVENTION

(4) The present invention will be described in detail below in combination with specific embodiments. The embodiments are used for illustrating the present invention, rather than limiting the scope of the present invention. Implementation conditions used in the embodiments can be further adjusted according to specific experimental environments; and the unspecified implementation conditions usually refer to conditions in the routine experiments.

(5) The present invention relates to a preparation method of a green, biodegradable, and multifunctional collagen-based nanocomposite film, which includes the following steps:

(6) (1) adding silicate nanosheet into deionized water for ultrasonic dispersion; then adding polyphenolic acid into the mixture, wherein a mass ratio of the polyphenolic acid to the silicate nanosheet is 1:(0.2˜1); and adjusting the pH value to 3.0˜4.0 to obtain a solution A, wherein the silicate nanosheet are any one of Laponite, montmorillonite, and kaolin; and the polyphenolic acid is any one of tannic acid, folic acid, and gallic acid;

(7) (2) adding collagen with a concentration of 5 g/L into an acetic acid solution, and fully dissolving the collagen to obtain a solution B;

(8) (3) isovolumetrically mixing the solution A with the solution B, stirring at room temperature, and adjusting the pH value to 4.5˜5.5 to obtain a casting solution;

(9) (4) pouring the casting solution into a polytetrafluoroethylene mold, and naturally drying to obtain a nanocomposite film.

Embodiment 1

(10) (1) adding Laponite nanosheet into deionized water for ultrasonic dispersion; then adding tannic acid into the mixture so that the concentration becomes 1 g/L; and adjusting the pH value to 3.5 to obtain a solution A, wherein a mass ratio of the tannic acid to the Laponite nanosheet is 1:0.2;

(11) (2) adding collagen with a concentration of 10 g/L into 0.5 mol/L of acetic acid solution, and fully dissolving the collagen to obtain a solution B;

(12) (3) isovolumetrically mixing the solution A with the solution B, stirring at room temperature, and adjusting the pH value to 5.0 to obtain a casting solution, wherein a mass ratio of the collagen to the tannic acid to the Laponite nanosheet is 10:1:0.2;

(13) (4) pouring the casting solution into a square polytetrafluoroethylene mold with a side length of 10 cm, and naturally drying to obtain a nanocomposite film.

Embodiment 2

(14) (1) adding montmorillonite nanosheet into deionized water for ultrasonic dispersion; then adding tannic acid into the mixture so that the concentration becomes 1 g/L; and adjusting the pH value to 3.5 to obtain a solution A, wherein a mass ratio of the tannic acid to the montmorillonite nanosheet is 1:0.4;

(15) (2) adding collagen with a concentration of 10 g/L into 0.5 mol/L of acetic acid solution, and fully dissolving the collagen to obtain a solution B;

(16) (3) isovolumetrically mixing the solution A with the solution B, stirring at room temperature, and adjusting the pH value to 5.0 to obtain a casting solution, wherein a mass ratio of the collagen to the tannic acid to the montmorillonite nanosheet is 10:1:0.4;

(17) (4) pouring the casting solution into a square polytetrafluoroethylene mold with a side length of 10 cm, and naturally drying to obtain a nanocomposite film.

Embodiment 3

(18) (1) adding kaolin nanosheet into deionized water for ultrasonic dispersion, then adding tannic acid into the mixture so that the concentration becomes 1 g/L, and adjusting the pH value to 3.5 to obtain a solution A, wherein a mass ratio of the tannic acid to the kaolin nanosheet is 1:0.6;

(19) (2) adding collagen with a concentration of 10 g/L into 0.5 mol/L of acetic acid solution, and fully dissolving the collagen to obtain a solution B;

(20) (3) isovolumetrically mixing the solution A with the solution B, stirring at room temperature, and adjusting the pH value to 5.0 to obtain a casting solution, wherein a mass ratio of the collagen to the tannic acid to the kaolin nanosheet is 10:1:0.6;

(21) (4) pouring the casting solution into a square polytetrafluoroethylene mold with a side length of 10 cm, and drying naturally to obtain a nanocomposite film.

Embodiment 4

(22) (1) adding Laponite nanosheet into deionized water for ultrasonic dispersion, then adding folic acid into the mixture so that the concentration becomes 1 g/L, and adjusting the pH value to 3.5 to obtain a solution A, wherein a mass ratio of the folic acid to the laponite nanosheet is 1:0.8;

(23) (2) adding collagen with a concentration of 10 g/L into 0.5 mol/L of acetic acid solution, and fully dissolving the collagen to obtain a solution B;

(24) (3) isovolumetrically mixing the solution A with the solution B, stirring at room temperature, and adjusting the pH value to 5.0 to obtain a casting solution, wherein a mass ratio of the collagen to the folic acid to the Laponite nanosheet is 10:1:0.8;

(25) (4) pouring the casting solution into a square polytetrafluoroethylene mold with a side length of 10 cm, and naturally drying to obtain a nanocomposite film.

EXPERIMENTAL EXAMPLE

(26) FIG. 1 is a diagram of a collagen-based composite film, wherein a control sample is a pure collagen film; FIG. 2 is a diagram showing test results of the transmittance of the collagen-based composite film; FIG. 3 is a diagram showing the results of the antioxidant property of a tannic acid-Laponite-collagen nanocomposite film. These figures show that the composite film prepared by the method of the present invention is biodegradable, and has high transmittance and significant antioxidant property.

(27) The above embodiments only exemplify principles and effects of the present invention. Those ordinary skilled in the art can make several modifications and improvements without departing from creative ideas of the present invention; and the modifications and improvements also fall within the protection scope of the present invention.