Qualitative and quantitative point-of-care assays

Abstract

Disclosed herein are equipment-free flow-through assay devices based on patterned porous media, methods of making same, and methods of using same. The porous, hydrophilic media are patterned with hydrophobic barriers for performing assays on liquids.

Claims

1. A capillary-driven device, comprising a hydrophobic detection reagent in a porous media, wherein the hydrophobic reagent changes to hydrophilic by the presence of a target analyte, and wherein the hydrophobic detection reagent is a compound of formula (I), ##STR00012## wherein, R.sup.1 and R.sup.2 are each independently, at each occurrence, selected from the group consisting of hydrogen, C.sub.1-C.sub.6-alkyl and C.sub.1-C.sub.6-alkoxy; x and y are each an integer independently selected from 0, 1, 2, 3, and 4; and n is an integer selected from 0 to 20.

2. The device of claim 1, wherein the device is a capillary-driven vertical flow-through device, a capillary-driven lateral flow-through device, or a combination thereof.

3. The device of claim 1, wherein the hydrophobic detection reagent is an oligomer or polymer.

4. The device of claim 1, wherein the hydrophobic detection reagent is selected from a carbamate, ether, polyether, a poly(phthalaldehyde), a polyvinyl carbamate, a polybenzyl carbamate, or a combination thereof.

5. The device of claim 1, wherein the hydrophobic detection reagent responds to hydrogen peroxide to convert from hydrophobic to hydrophilic molecules.

6. The device of claim 1, wherein R.sup.1 and R.sup.2 are each C.sub.1-C.sub.6-alkoxy.

7. The device of claim 1, wherein R.sup.1 and R.sup.2 are each methoxy.

8. The device of claim 1, wherein n is an integer selected from 0 to 20.

9. The device of claim 1, wherein an assay region of the device comprises one or more responsive agents that select for specific analytes.

10. The device of claim 1, comprising an assay region derivatized with a substrate.

11. The device of claim 1, comprising an immobilized enzyme.

12. The device of claim 1, comprising a first lateral flow channel leading to a first vertical flow column containing a plurality of treated layers; and a second lateral flow channel leading to a second vertical flow column containing a plurality of treated layers.

13. The device of claim 12, wherein the second vertical flow column is configured to correct for internal and/or external factors that affect the rate of the sample within the device, except for the effect of the target analyte on the rate.

14. The device of claim 1, comprising a layer containing a dye for visualization of the sample in a visualization layer, wherein the dye in the dye layer is separate from the hydrophobic detection reagent.

15. The device of claim 1, wherein the concentration of an analyte in a sample is determined by measuring the time for the sample to flow through the device after addition of the sample.

16. The device of claim 1, wherein the concentration of an analyte in a sample is determined by visually counting the number of analyte-dependent bars after addition of the sample.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1A illustrates a layout of a vertical flow-through assay device. FIG. 1B illustrates a detection reagent, along with the mechanism for conversion from hydrophobic to hydrophilic molecules. FIG. 1C illustrates the results of a quantitative determination of the presence of hydrogen peroxide in a sample. Three different quantities of the detection reagent were used to generate the three curves shown. FIG. 1D illustrates changes in sensitivity by altering the quantity of detection reagent within a vertical flow-through assay device.

(2) FIG. 2 illustrates variations to the initial detection reagent that can be used to change the sensitivity of an assay.

(3) FIGS. 3A and 3B are perspective views of flow-through assay devices. FIGS. 3C and 3D illustrate the results of a quantitative determination of alkaline phosphatase (FIG. 3C) or -D-galactosidase in a sample. The selectivity of the assays performed is evident by the lack of a response when enzymes other than the target were tested.

(4) FIG. 4 illustrates the removal of glucose and hydrogen peroxide from a sample, containing the target enzyme, through the addition of preprocessing layers.

(5) FIG. 5A illustrates the layout of a vertical flow-through device. FIG. 5B illustrates the results of a quantitative determination of hydrogen peroxide in a sample.

(6) FIG. 6A shows a digital assay that uses a single conduit device. FIG. 6B shows an analog assay that uses a radial paper-based microfluidic device. FIG. 6C shows a detection reagent.

(7) FIG. 7A shows a diagram of an assay procedure for a digital device. FIG. 7B shows photographs over time for a device exposed to an analyte.

(8) FIG. 8 shows the effect of the quantity of 180 per volume of paper (m) on the flow-through time of water through a digital device.

(9) FIGS. 9A and 9B show the effect of analyte concentration on flow-through time.

(10) FIGS. 10A-10E show an analog device.

(11) FIG. 11 shows the sensitivity of an analog embodiment of the device.

(12) FIG. 12 illustrates the layout of a vertical flow-through assay device.

(13) FIGS. 13A and 13B show the relationship between a detection reagent and the time required for a sample containing analyte to flow through a paper-based microfluidic device.

(14) FIG. 14 illustrates increasing the sensitivity of a quantitative assay by modifying the detection reagent.

(15) FIG. 15 shows the limit of detection as a function of detection reagent repeat units.

(16) FIG. 16 illustrates the layout of a vertical flow-through assay device.

(17) FIGS. 17A and 17B show the layout of a vertical and horizontal flow-through assay device. FIGS. 17C and 17 D show an enzyme assay detection reagent.

(18) FIGS. 18A and 18B show calibration curves for devices comprising enzyme assays.

(19) FIG. 19 shows the effect of humidity and temperature on the accuracy of assay devices.

(20) FIG. 20 illustrates a flow-through assay device.

(21) FIGS. 21A and 21B show detection of adenosine as a model target to demonstrate quantitative detection of an analyte using an aptamer-based device.

(22) FIG. 22 illustrates a multiplexed flow-through assay device.

(23) FIG. 23 depicts heavy metal analysis by a multiplexed flow-through assay device.

(24) FIGS. 24A-24C illustrate a multiplexed flow-through assay device, wherein heavy metal analysis can be performed.

(25) FIG. 25 shows an aptamer-based reagent for a flow-through assay device.

(26) FIG. 26 shows a detection reagent used, along with the mechanism for conversion from hydrophobic to hydrophilic molecules.

(27) FIGS. 27A and 27B show effects of preproccesing on an assay for adenosine using samples spiked with either glucose or hydrogen peroxide.

(28) FIGS. 28A-28D show calibration curves for heavy metal analysis.

(29) FIG. 29 shows heavy metal analysis of different samples.

DETAILED DESCRIPTION

(30) Disclosed herein are equipment-free flow-through assay devices based on patterned porous media, methods of making same, and methods of using same. The assay devices can be capillary-driven devices that include a hydrophobic detection reagent in a porous media. The hydrophobic reagent changes to hydrophilic by the presence of a target analyte.

(31) Porous, hydrophilic media can be patterned with hydrophobic barriers for performing assays on liquids. One example of a useful hydrophilic medium for assays is paper, which is inexpensive, disposable, wicks liquids rapidly, and does not require special handling procedures. The paper or other porous, hydrophilic medium can be patterned with hydrophobic barriers that provide spatially defined regions for fluid transport based on capillary action. These hydrophobic barriers, such as wax, can provide an impermeable barrier throughout the entire thickness of the porous, hydrophilic medium within defined areas. The regions defined by the hydrophobic barriers contain the hydrophilic, porous medium, rather than being empty as is common in glass or polymeric (PDMS) microfluidic devices.

(32) Detection Reagents

(33) The hydrophobic detection reagent can be a responsive hydrophobic oligomer. The detection reagent can be a responsive small molecule, oligomer or polymer, including, but not limited to, polyethers, poly(phthalaldehyde), polyvinyl carbamates, and other polycarbamates (e.g., polybenzyl carbamates). One example is an aryl boronate (referred to as the end-cap) protected oligomeric polycarbamate that is hydrophobic. FIG. 1B shows the structure 180 of a hydrophobic detection reagent and the conversion to hydrophilic byproducts in response to hydrogen peroxide (the target analyte). The aryl boronate end-cap is oxidatively cleaved in the presence of hydrogen peroxide to reveal a phenol. Through a quinone methide elimination, an electron cascade occurs along the oligomer backbone to generate hydrophilic small molecule byproducts. In some embodiments, the sensitivity of the detection reagent within the device is modified by increasing the rate of depolymerization through the inclusion of a methyl ether, 200, within each repeating unit (FIG. 2). The methyl ether destabilizes the benzylic CO bond, increasing the rate of quinone- and azaquinone methide elimination. In some embodiments, the sensitivity is improved by creating a larger change from hydrophobic to hydrophilic in response to a single detection event (Table 1). Longer oligomers will be more hydrophobic (210-240), yet produce the same byproducts, thereby amplifying the response of the detection reagent for a single detection event. The electronics of the detection reagent can be modified (i.e., naphthalene-based repeating units, additional methyl ethers or other electron donating groups, addition of alkyl chains to affect solubility, etc.) to tailor the sensitivity of the device. Other detection reagents that change from hydrophobic to hydrophilic or hydrophilic to hydrophobic can be used in the context of the device, such as responsive polymers (e.g., poly(phthalaldehyde)). The responsive end-cap used can include, but are not limited to, aryl boronate, TBS, FMoc, and Alloc protecting groups in order to provide selectivity for target analytes.

(34) Responsive Reagents

(35) In some embodiments, to perform an assay, an assay region of the hydrophilic medium can be derivatized with responsive reagents such as (but not limited to) small molecules, signal transduction molecules, substrates, aptamers, antibodies, or proteins, that interact with the target analyte. For example to detect hydrogen peroxide, an assay region of the hydrophilic medium can be derivatized with a hydrophobic small molecule detection reagent that selectively reacts with hydrogen peroxide. The presence of the hydrophobic detection reagent modifies the wetting properties of the assay region, converting the hydrophilic, porous medium to hydrophobic. When the hydrophobic detection reagent reacts with hydrogen peroxide, it converts to hydrophilic byproducts, switching the wetting properties of the assay region to hydrophilic. In another example, a plurality of assay regions, derivatized with different responsive reagents, can be used in order to detect a target enzyme, such as alkaline phosphatase. In the first assay region encountered by the sample, the assay region is derivatized with a substrate, such as glucose 6-phosphate, that selectively reacts with the target enzyme to produce glucose. Following the production of glucose, the sample can encounter an assay region containing an immobilized enzyme (immobilized on a bead or on the paper itself), such as glucose oxidase, that generates hydrogen peroxide from the glucose within the sample. The hydrogen peroxide is detected with an assay region derivatized with a hydrophobic small molecule detection reagent that selectively reacts with hydrogen peroxide. The presence of the hydrophobic detection reagent modifies the wetting properties of the assay region, converting the hydrophilic, porous medium to hydrophobic. When the hydrophobic detection reagent reacts with hydrogen peroxide, it converts to hydrophilic byproducts, switching the wetting properties of the assay region to hydrophilic. In general a wide variety of reagents can be used in assay devices (i) to detect analytes, (ii) to modify the pH of the sample solution, (iii) to modify the wetting properties of the hydrophilic, porous medium, (iv) to generate additional reagents necessary for the assay to be performed, or (v) to interact with other responsive reagents in order to initiate a signal transduction pathway (e.g., a thiol interacting with a disulfide within another responsive reagent). These reagents can include, but are not limited to, antibodies, aptamers, responsive polymers, proteins, salts, or organic small molecules. These reagents could be adsorbed to the porous, hydrophilic medium non-covalently (through non-specific interactions) or covalently.

(36) Device Assembly

(37) In some embodiments, the vertical flow-through assay device includes multiple alternating layers of a porous, hydrophilic medium that is patterned with hydrophobic barriers and protective coatings (or insulating material) that hold the layers of the device in contact, such as double sided tape, adhesive, or laminate. The fluid flows vertically from one layer to another, constrained by the patterned hydrophobic barriers. A plurality of the defined areas of porous, hydrophilic medium can be treated prior to assembly of the device to provide an assay for a target analyte. Some embodiments may include both lateral and vertical flow-through of the liquid.

(38) Fixed Sample Volume

(39) To obtain a quantitative measurement of an analyte in a sample, a fixed volume of liquid can be deposited in the device. In some embodiments, a defined volume of fluid (or a volume approximately close to the defined volume) can be obtained through patterning the hydrophilic, porous medium using hydrophobic barriers. The hydrophobic barriers generate microchannels, sample wells, or regions that accept a fixed volume of liquid.

(40) TABLE-US-00001 TABLE 1 Effect of oligomer length and electronics on limit of detection for quantifying hydrogen peroxide within a sample. Compound Number of Repeating Units Limit of Detection (M) 180 0 3125.4 200 0 168.5 210 1 24.8 220 2 11.8 230 5 3.7 240 8 3.2
Spotting Reagents

(41) In some embodiments, reagents can be spotted using capillary tubes and pipets. Ink jet printing and pins, such as used in microarrays, can be used to deposit reagents for mass-production. The reagents can be spotted using organic and/or aqueous solutions. The spotted reagents can be allowed to air dry at room temperature for at least 30 minutes before using the device. The reagents can be dried under vacuum as well.

(42) Flow-Through Device

(43) In some embodiments, the device, 100, is a hydrophilic column made up of a plurality (e.g., 3 to 6 layers) of layers of patterned hydrophilic, porous media with a central, circular defined region (FIG. 1A). A plurality of layers contain regions that are treated with reagents for an assay prior to assembly of the device. The sample (e.g., an aqueous sample, or other type of fluid-containing sample) is added to the top layer, 110, of the device, at the sample inlet region, 115; when the sample is added the assay time is started. The sample wicks vertically, due to capillary action, to the next layer, 120. The second layer, 125, is treated with buffer salts (HEPES) to control the pH of the assay solution. The sample then wicks vertically to contact a plurality (e.g., 1 to 4 layers) of layers, 130-150, containing a region treated with the detection reagent, 135-155. The region treated with the detection reagent alters the porous media to inhibit the flow of liquid through the layer (become hydrophobic) when hydrogen peroxide is not present within the sample. When hydrogen peroxide is present, the detection reagent converts to hydrophilic byproducts, switching the porous media from hydrophobic to hydrophilic (FIG. 1B). The rate that the sample is able to convert the detection reagent to hydrophilic depends on the concentration of hydrogen peroxide in the sample, thereby correlating the time for the sample to wick through the plurality of layers treated with the detection reagent to the concentration of hydrogen peroxide. The sample then encounters a layer, 160, that has been treated with a dye, to allow the sample to be visualized, 165. The colored sample wicks to the bottom layer (170), the visualization layer, causing the visualization layer to change color, 175. The change in color of the visualization layer stops the assay time. The time from addition of the sample to the change in color of the visualization layer is the assay time, and will correlate to the concentration of hydrogen peroxide within the sample (FIG. 1C). The number of layers within the device affects the volume of sample required for the assay to perform optimally, however the assay requires very small volumes of sample for all instances (e.g., 8 to 12 L). Changes of the quantity of detection reagent, 180, affect the sensitivity of the assay (FIG. 1D) and can be altered to tune the sensitivity to the desired levels for the assay.

(44) Enzyme Assay

(45) In some embodiments, the device (300) contains a central column of hydrophilic, porous media that splits the sample into two lateral flow channels leading to two separate vertical flow columns containing a plurality of treated layers where an assay is performed (FIGS. 3A and 3B). The device is made from hydrophilic, porous media (e.g., paper) patterned with hydrophobic barriers and layers of adhesive material (e.g., spray adhesive) stacked in alternating order. Protective coatings (e.g., laminate) are used to seal the top and bottom of the device (together), with an aperture in the top layer of the protective coating to allow for sample addition. The sample (e.g., 30 to 120 L) is added to the sample addition aperture (312), contacting the top layer of patterned paper, 310. The sample then wicks vertically through a plurality of layers (e.g., 2 to 6 layers), 322 and 332, before splitting into two sides (the assay side and the control side) via a horizontal lateral flow channel, 342, that contains buffer salts (e.g., HEPES). The sample wicks down vertically in both sides (352), encountering a substrate (e.g., glucose 6-phosphate) for the target analyte (e.g., alkaline phosphatase) in a plurality of layers (e.g., 1 to 4 layers), 350 and 360. When alkaline phosphatase (the target analyte) is present within the sample, the glucose 6-phosphate (the substrate) is converted to glucose. In both sides, the sample then reaches a lateral flow region containing additional substrate, 370. In the assay side of the device only (372), this lateral flow region also contains an immobilized enzyme (e.g., glucose oxidase immobilized on 10 m polystyrene particles). The immobilized glucose oxidase will convert the glucose generated by the target enzyme into hydrogen peroxide. The size of the polystyrene particles prevents the glucose oxidase from travelling with the solvent of the sample, minimizing the amount of protein present within the sample (high levels of protein will affect the viscosity and wicking rate of a liquid). The sample then wicks up vertically through identical columns of patterned paper on both sides. The sample encounters a plurality (e.g., 1 to 4 layers) of layers, 361 and 351, containing detection reagent that will respond to the presence of hydrogen peroxide. Following the detection reagent layer(s), the sample redissolves a dye (321) and then wicks to the top layer into the visualization regions, 311. The assay time is started when the visualization region of the assay side changes color, and ends when the visualization region of the control side changes color. The measured assay time will correlate to the initial concentration of alkaline phosphatase within the added sample. The comparison of flow-through time between the assay side and the control side directly measures the generation of hydrogen peroxide from the presence of the alkaline phosphatase, 380 (FIG. 3C). The responsive substrate used within the device (352, 362, 371, and 372) provides the selectivity for this type of assay. Changing the substrate allows for selective detection of different analytes (e.g., -D-Galactosidase, 381) without showing a response to other enzymes (e.g., catalase, 382). Due to the lack of glucose oxidase, the control side should not generate any hydrogen peroxide, even when alkaline phosphatase is present. The control side, 371, serves to correct for any internal (i.e., viscosity, or background hydrogen peroxide) or external factors (i.e., temperature, or humidity) that may affect the wicking rate of the sample within the device.

(46) Preprocessing

(47) In some embodiments, the device contains layers to remove impurities from the sample that may affect the assay results. The impurities removed may be physical (e.g., dirt) or chemical (e.g., glucose or hydrogen peroxide). The porous media used in the device can be used to filter particles from the sample. In some embodiments, the porous medium used can be selected to filter the sample and remove physical impurities, such as red blood cells. The pore size of the porous media used will determine the particle size that can be removed from the sample. Treatment of the hydrophilic, porous media can bind, or otherwise remove small chemical impurities that cannot be removed based on size. In certain embodiments, the device contains a layer(s) treated with immobilized glucose oxidase (322), followed by a subsequent layer(s) treated with immobilized catalase (332). Glucose that is present within the sample will be converted to hydrogen peroxide in the layer containing glucose oxidase, the generate hydrogen peroxide will then be decomposed by the catalase, eliminating glucose, 510, present within the sample (FIG. 4). This same setup allows for the removal of background hydrogen peroxide, 500, within the sample as well. Enzymes can be immobilized either through chemical modification of the porous media, or through coupling (e.g., biotin-streptavidin) with large particles (e.g., 10 m polystyrene particles). The pore size of the hydrophilic, porous media prevents the movement of the immobilized enzymes between layers.

(48) Dial Design

(49) In some embodiments, the device functions using a fixed assay time (e.g., 5, 10, 15, or 20 minutes), rather than measuring concentration by the final assay time. One example of the device, 500, contains a central sample addition column, surrounded by a circular array (e.g., 2 to 25 columns) of equally spaced assay columns. The sample is added to the top layer, 510, of the device, in the sample addition region (511), and then wicks down vertically through a plurality (e.g., 4 to 8 layers) of layers, 520-560. The bottom layer, 570, of the device laterally distributes, 571, the sample evenly to each of the surrounding assay columns. The sample then wicks up vertically into each column simultaneously, 562-512. In each column, the sample encounters a layer, 562, treated with buffer salts (e.g., HEPES) before contacting a plurality (e.g., 1 to 4 layers) of layers, 552 and 532, treated with the hydrophobic detection reagent. The presence of the analyte (e.g., hydrogen peroxide) converts the hydrophobic detection reagent to hydrophilic byproducts. In a clockwise manner, each subsequent assay column has an increasing quantity of the hydrophobic detection reagent initially included, starting at the top of the device (12 o'clock position). The sample then wicks through a layer, 522, treated with a dye before reaching the top layer, containing visualization regions, 512, for each column. Within a fixed period of time, the concentration of hydrogen peroxide initially within the sample will only convert a certain quantity of the hydrophobic detection reagent to hydrophilic. The assay columns containing less than or equal to this quantity of hydrophobic detection reagent will have allowed the sample to reach the visualization layer within the fixed assay time, whereas the columns containing more detection reagent than this will not. The concentration of hydrogen peroxide within the sample is measured by counting the number of visualization regions on the top of the device that have changed color at the end of the fixed assay time (FIG. 5B). In one embodiment of the device, the visualization regions are shaped like bars, so the device can be read similar to a dial. Other configurations of the device, including but not limited to, multiple arrays of visualization regions, different shaped arrays of visualization regions, and inclusion of a fluidic timer, can be used in order to tune the sensitivity, selectivity, and ease of use for the device.

(50) Multiplexed Assays

(51) In some embodiments, multiple assays can be performed simultaneously in a single device, using the same aliquot of sample. The single addition of a sample allows the user to perform multiple assays without additional processing, improving the ease of use for the device. One example includes a device where the sample is added to the top layer of the device, in the sample addition region, before wicking to the distribution layer. The distribution layer laterally distributes the sample evenly to two assay device regions. The sample then wicks down vertically into the two assay device regions, which are substantially identical to each other, but with the responsive reagents necessary to detect two distinct analytes (e.g., glucose 6-phosphate for alkaline phosphatase and lactose for -D-galactosidase, as well as aptamers for the detection of heavy metals). The layout of each of the assay device regions can be similar to 300, or a different configuration, depending the analytes detected, and the method of detection used (e.g., flow-through device, enzyme assay, or dial device).

EXAMPLES

(52) The foregoing may be better understood by reference to the following examples, which are presented for purposes of illustration and are not intended to limit the scope of the invention.

Example 1. Capillary-Driven Device Using Small Molecule Detection Reagents

(53) Capillary-driven devices using small molecule detection reagents were constructed and evaluated. FIGS. 6A-6C depict two 3D paper-based microfluidic devices including compound 1 as a detection agent.

(54) ##STR00007##

(55) Both of the assays are based on selective changes in the wetting properties of paper, since hydrogen peroxide (a model analyte) oxidatively cleaves compound 1 (the detection reagent; FIG. 6C), which is hydrophobic and is deposited into defined regions of the microfluidic conduits prior to assembling the devices. Reaction of hydrogen peroxide with compound 1 initiates an elimination reaction that converts compound 1 into hydrophilic byproducts such as compounds 2 and 3 (FIG. 6C). This change from hydrophobic to hydrophilic allows the sample to wick through the device and wet a detection region, where the time required to wet the detection region depends on the concentration of the analyte.

(56) FIG. 6A shows a digital assay that uses a single conduit device to report the concentration of hydrogen peroxide by measuring the time required for the sample to flow through the device in the z direction. This 3D paper-based microfluidic device of FIG. 6A (10 mm wide10 mm long0.9 mm thick) is formed by stacking alternating layers of wax patterned paper and patterned double-sided adhesive tape to create a device that contains seven layers (four paper layers and three tape layers) and a single hydrophilic conduit that extends in the z direction from one end of the device to the other. 2 L of sample is added to the top of the hydrophilic conduit (layer 1) and a colored readout appears on the bottom of the device (layer 7) when the sample has flowed through the entire device (FIG. 7). Layer 2 is patterned tape that contains a 2.5 mm diameter hole that is filled with a 180 m thick disk of Whatman Chromatography Paper No. 1. This disk contains 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (HEPES) that was predeposited (0.25 L from a 45 mM, pH 8.0 solution) and dried on the paper disk prior to assembling the device. The buffer salts are included in the device to ensure that the pH value of the sample is approximately 8.0, which facilitates rapid oxidative cleavage of 1 by hydrogen peroxide. Layer 4 contains a disk of Whatman Chromatography Paper No. 1 in a hole in patterned tape, whereas layers 3 and 5 are patterned Boise Aspen 30 printer paper that each contain compound 1 in a 2.5 mm diameter hydrophilic region (1 is predeposited from ethyl acetate solutions ranging in concentration from 7.5 to 130 mM). Two layers that contain 1 are included, because initial studies showed that two layers provided more reproducible assay results than a single layer of paper containing 1. Layer 6 is patterned tape, but in this case the 2.5 mm diameter, 180 m thick disk of Whatman Chromatography paper No. 1 contains predeposited (0.25 L) green food coloring. Layer 7 is the readout region and contains patterned Boise Aspen 30 printer paper with a 2.5 mm diameter white hydrophilic region that becomes bright green after the sample redissolves the dye in layer 6 and distributes it to layer 7.

(57) FIGS. 7A and 7B depict a diagram of the assay procedure for the digital device in FIG. 6A exposed to 100 mM hydrogen peroxide. The digital device of FIG. 7A-7B may function as follows: sample is added initially to the top of the device, the device is then inverted to allow the detection layer to become visible. After the sample is added to layer 1 (FIG. 7A, left images), it wicks through layers 1 and 2, buffering the sample solution with the redissolved HEPES. Once the sample reaches layer 3, hydrogen peroxide in the sample reacts with 1 to yield hydrophilic products (FIG. 7A, middle images). This reaction is repeated in layer 5. The reaction with hydrogen peroxide changes the wetting properties of the paper to allow the sample to continue wicking through the device to layer 6, where it redissolves the green food coloring from the paper disk. This food coloring is then wicked into layer 7 where the colored solution becomes visible (FIG. 7A, right images).

(58) The addition of pure water to this type of digital device demonstrates the effect that compound 1 has on the wetting properties of paper (FIG. 8, the error bars reflect the standard deviations from the average values) of the device in FIG. 6A. The average flow-through time for each experiment in FIG. 8 was determined by using at least five replicate measurements at 20 C. and 25% relative humidity. Separate experiments were conducted using different amounts of compound 1; from these experiments it was found that the presence of only 3.6 g mm.sup.3 of compound 1 in layers 3 and 5 is needed to prevent water from flowing through the vertical hydrophilic conduit in (e.g., as shown in FIG. 1A or FIG. 6A) within the allotted assay time of 45 min.

(59) When hydrogen peroxide is included in the sample, however, the flow-through time is correlated with the concentration of hydrogen peroxide in the sample (FIG. 9A, closed circles). Specifically, devices were assembled as shown in FIG. 6A; layers 3 and 5 each contained 1.7 g mm.sup.3 of compound 1. The open circles represent flow-through times when 0 g mm.sup.3 of compound 1 is included in the device. The average flow-through time for each concentration of hydrogen peroxide was determined using ten replicate measurements at 20 C. and 25% relative humidity. FIG. 9B shows an expanded view of the dotted region in 9A. This graph was used to determine the limit of detection for the assay. The error bars reflect the standard deviations from the average values. The measured flow-through times were recorded when the entire hydrophilic region of layer 7 turned green, which required between less than 1 second and 10 seconds from the initial appearance of green. Low concentrations of hydrogen peroxide had the slowest flow-through times and the greatest delay in filling layer 7 with the green solution.

(60) The exponential relationship between hydrogen peroxide concentration and flow-through time illustrated in FIG. 9A indicates that the assay is particularly sensitive to low concentrations of hydrogen peroxide: in this assay, the limit of detection is approximately 0.7 mM hydrogen peroxide (FIG. 9B) and the dynamic range is approximately 0.7-100 mM. FIG. 9A also reveals that the detection reagent (e.g., compound 1) is essential for generating the aforementioned relationship between hydrogen peroxide concentration and flow-through time: as can be seen in the absence of the detection reagent (FIG. 9A, open circles), the flow-through times are uniform and rapid across the different concentrations of hydrogen peroxide tested.

(61) Since wicking rates in porous media typically are affected by humidity, the effect of this variable was tested in the flow-through assay. The flow-through time is independent of humidity over the range of 17-62% relative humidity, which is a result that can be attributed to the exceedingly short path length that the sample must travel in the device (ca. 900 m).

(62) FIG. 6B and FIG. 10A show an analog assay that uses a radial paper-based microfluidic device for quantifying the level of hydrogen peroxide by using a fixed assay time and by counting the number of bars that become colored as a function of the concentration of hydrogen peroxide in the sample. This analog design (FIG. 6B) requires that a user count the number of colored bars that appear after a fixed assay time. Sample is initially added to the top of the device, where it wicks down through the device and is distributed to the surrounding conduits. These surrounding conduits have nearly the same configuration as the device in FIG. 6A, with the exception that the quantity of the detection agent (e.g., compound 1) increases by 1.1 g mm.sup.3 in each subsequent conduit along the radial configuration. FIGS. 10B-10E depict photographs after a 10 min assay of devices that were exposed to 1 mM (FIG. 10B); 35 mM (FIG. 10C); 75 mM (FIG. 10D); and 100 mM hydrogen peroxide (FIG. 10E). The edges of the devices shown are marked with white dotted lines. Specifically, the design uses an 8-layer 3D paper-based microfluidic device (32 mm wide32 mm long1.0 mm thick) that contains 16 hydrophilic conduits (2.5 mm diameter) arranged in a circle surrounding a central hydrophilic conduit (10 mm diameter). The sample is added to the central conduit in layer 1, which is patterned Whatman Chromatography Paper No. 1, and then wicks through the device in the z direction down to layer 7 (FIG. 10A, top image). In layer 7, the central conduit separates the sample evenly into the 16 surrounding conduits. The surrounding conduits direct the sample up (in the z direction), opposite to the direction of flow in the central conduit. This arrangement allows the sample to be added to the top of the device, and the bars for the dial to be visible on the top as well. Layer 8 is a single-sided protective tape that allows the user to place the device on a surface to run the assay. The outer conduits of this device are composed of the same layers as the conduit in the digital device (e.g., a paper disk containing HEPES buffer salts, followed by two layers containing 1, and then a paper disk containing dried green food coloring). However, in this device, each successive conduit contains increasing amounts of compound 1 compared to the previous conduit (FIG. 10A). Because flow-through time increases as the amount of compound 1 in the conduit increases, each conduit has a longer flow-through time than the preceding one. By using a fixed assay time (e.g., 10 min), the number of colored bars (resulting from flow-through) is used to quantify the amount of hydrogen peroxide in a sample. FIGS. 10B-10E show several examples of devices that were exposed to different concentrations of hydrogen peroxide.

(63) FIG. 11 shows the relationship between the number of bars filled (n) in the analog device and the concentration of hydrogen peroxide (0-100 mM) in a sample. The concentration of compound 1 increased by 2.3 g mm.sup.3 increments between conduits in the devices used to obtain this data. The assay may be more sensitive if conduits increase in 2.3 g mm.sup.3 increments rather than 1.1 g mm.sup.3. The average number of bars after a 10 min assay was obtained from five replicate measurements. The error bars reflect the standard deviations from the average values.

(64) While these studies used hydrogen peroxide as a model analyte for demonstrating the use of analog and digital assays in paper microfluidics, other analytes may be used as well.

Example 2. Capillary-Driven Device Using Oligomer Detection Reagents

(65) Capillary-driven devices using oligomer detection reagents 3-6 were constructed and evaluated.

(66) ##STR00008##

(67) The carbamate oligomers can depolymerize from head-to-tail as phase-switching reagents and be used for increasing the sensitivity of quantitative point-of-care assays that are based on measurements of time. The carbamate oligomers selectively react with hydrogen peroxide (a model analyte) and provide sensitivity by depolymerizing in the presence of the analyte to convert from water-insoluble polymers to water-soluble products. This switching reaction allows a sample to wick through a three-dimensional paper-based microfluidic device, where the flow-through time reflects the quantity of the analyte in the sample. Oligomers as short as pentamers allow quantitative detection to low nanomolar concentrations of the analyte. This approach improves the sensitivity four orders of magnitude compared to devices of Example 1 (limits-of-detection of hydrogen peroxide of Example 2 are approximately 146 nM). By further optimizing the number of layers of paper containing the oligomer in the assay platform, the limit of detection was improved by approximately another order of magnitude, providing a limit of detection of 31 nM and a 50,000-fold improvement. The assay devices can be used in resource-limited environments where many analytes of interest are present in samples at (or below) micro- and nanomolar levels.

(68) The devices including oligomeric detection reagents were prepared according to FIG. 12. The assays, like that of Example 1, are conducted in a three-dimensional (3D) paper-based microfluidic device that includes stacked, alternating layers of (i) paper that has been patterned with wax into hydrophobic and hydrophilic regions, and (ii) double-sided adhesive tape, which has holes patterned into it using a CO.sub.2 laser cutter, as shown in FIG. 12. The holes in the tape are filled with disks of hydrophilic paper such that hydrophilic regions in paper connect with the hydrophilic disks in the tape. Each layer of the device (except layers 1, 4, and 7) is pre-loaded with a reagent and dried before assembly, such that addition of sample to the top of this device allows the assay to occur automatically without user intervention. As the sample passes from layer 1 into layer 2, it re-dissolves HEPES buffer salts to control the pH of the sample. In layers 3 and 5, the sample encounters the detection reagent (e.g., an oligomer compound 3, 4, 5, or 6), which is hydrophobic and water-insoluble, thus alters the wetting properties of the paper. In the absence of the analyte, the sample stops wicking (or slowly wicks, depending on the quantity of oligomer detection reagent in the paper) through hydrophobic layers 3 and 5. In the presence of hydrogen peroxide, the oligomer detection reagent (e.g., compound 3, 4, 5, or 6) selectively degrades into hydrophilic products, thus switching phases from insoluble into soluble products, and, consequently, changing the wetting properties of the paper so that it is once again hydrophilic. After this switching reaction occurs, the sample wicks to layer 6 where it re-dissolves dried food coloring to convert the sample into a brightly colored solution, which becomes visible when the sample fills the hydrophilic circular region in layer 7. The quantity of hydrogen peroxide is measured in this device by tracking the time required for the sample to pass from the top of the device to the bottom, which is established by the appearance of green color in layer 7. The selectivity for the assay is provided by the selective oxidative cleavage of the aryl boronate in the oligomer detection reagent via hydrogen peroxide, although presumably other activity-based detection events could be employed if the aryl boronate is replaced with a substrate for another target analyte.

(69) Design of the oligomers (FIG. 14B) was based on compound 2 (FIG. 14A), which itself was designed based on compound 1 and the reaction kinetics/limit of detection profile of compound 1 (FIGS. 13A and 13B). Specifically, compound 2 was designed to degrade into hydrophilic products faster than compound 1. Addition of a methyl ether ortho to the benzylic leaving group on the benzene ring accelerates the rate of quinone methide elimination reactions, which is the proposed degradation pathway for compounds 1 and 2 to convert to hydrophilic products. Accelerating this quinone methide elimination reaction, it was hypothesized that more of hydrophobic compound 2, compared to compound 1, would convert to hydrophilic products in the time frame that it takes for (i) hydrogen peroxide to react with compound 2 and (ii) for the aqueous solution to pass through layers 3 and 5 in the device. If more of compound 2 converts to hydrophilic products than compound 1 within the time frame of flow-through, then less hydrogen peroxide would be needed to allow flow-through using compound 2, increasing the sensitivity for the assay. Oligomers modeled after compound 2 provide a greater change in hydrophobicity than compound 2 by converting from a large hydrophobic molecule (an oligomer) to small hydrophilic products upon reaction with hydrogen peroxide, further increasing the sensitivity for the assay, since the change in wetting properties will be amplified relative to compound 2. The proposed mechanism of response for the oligomers is as follows: oxidative cleavage of the aryl boronate in compounds 3-6 would generate phenol, which would initiate a cascade head-to-tail depolymerization reaction through quinone- and azaquinone-methide-mediated pathways, similar to the mechanism depicted for compound 1 in FIG. 1B.

(70) ##STR00009##

(71) Compound 2 was prepared from 4-(hydroxymethyl)-3-methoxyphenylboronic acid pinacol ester and p-nitrophenyl isocyanate. Compounds 3 and 4 were prepared in a step-wise fashion, while oligomers 5 and 6 were prepared according to the route outlined in Scheme 1. This route involved a tin-catalyzed polymerization of monomer 8, wherein the length of the oligomer was controlled by polymerization time. The polymerization reaction was quenched by addition of the aryl boronate end-cap, and a postpolymerization modification was used to append the p-nitrophenyl carbamate.

(72) ##STR00010##

(73) Addition of a methyl ether (such as the methyl ether in compound 2 vs. compound 1) can accelerate the rate of quinone- and azaquinone-methide-mediated release of a benzylic group by as much as 40 compared with a derivative that lacks the methyl ether. After optimizing the quantity of 2 needed for this quantitative flow-through assay, the anticipated difference in rate between compound 1 and compound 2 provided a 17-fold improvement in the sensitivity for the assay (i.e., LOD=1707 M for 1 vs. 103 M for 2). Formation of quinone methide can be a rate-limiting step under the conditions of the assay.

(74) Oligomers 3-6 impart significant improvements in sensitivity to the assay compared to 1 and 2 (FIG. 15). For example, after optimizing the quantity of each reagent for the flow-through assay, it was found that addition of one repeating unit (e.g., oligomer 3) improved the limit of detection 6 compared to 2 and 107 compared to compound 1. In fact, a nearly linear relationship exists between the limit of detection and the number of repeating units in the oligomer until, presumably, the rate of hydrogen peroxide-induced depolymerization becomes competitive with the residence time of the sample within layers 3 and 5 of the device (FIG. 15). Incomplete depolymerization would affect the magnitude of hydrophobic-to-hydrophilic phase-switching since the depolymerization intermediates (i.e., truncated oligomers) are expected to retain substantial hydrophobic character relative to the products of complete depolymerization. There appears to be a match in depolymerization time and flow-through time when approximately 2-5 repeating units are added to compound 2, at which point less dramatic improvement in sensitivity is obtained when using longer oligomers that depolymerize more slowly than oligomer 4 (e.g., oligomer 6, n=8, has a LOD=0.15 M, whereas 5, n=5, has a LOD=0.96 M).

(75) Devices were prepared with one, two, or three layers of paper that were modified with oligomer 3. The relationship between the limit of detection and the total quantity of oligomer 3 was then characterized within the devices to determine the minimum quantity of 3 needed to provide the lowest limit of detection. These experiments revealed that a device containing one layer of oligomer 3 provided a limit of detection for hydrogen peroxide that is nearly 5 better than a comparable device containing three layers of oligomer 3 (e.g., the LOD for one layer of 3==9.4 M, while three layers of 3=46 M). Specifically, the limit of detection worsens by 2 every additional layer of oligomer 3 incorporated into the device. Likewise, the dynamic range for the assay worsens as the number of layers containing oligomer 3 increases. For example, the device containing one layer of oligomer 3 has a dynamic range of 9.7 M to 1000 M, whereas the device containing three layers of oligomer 3 has a smaller dynamic range of 57 M to 500 M. A device with improved sensitivity can provide a single layer where the magnitude of phase-switching is substantial, rather than providing small stages of phase-switching over several layers in a device.

(76) A single layer device was produced, and was used to determine the optimum quantity of oligomer 5 (the oligomer that provided the best limit of detection) needed to provide the lowest limit of detection for quantifying hydrogen peroxide in a sample. This assay requires only 3.4 g of oligomer 5, yet now provides measurements of hydrogen peroxide down to 31 nM, which is a LOD that is 55,000 better than the aformentioned assay, as well as a useful dynamic range.

(77) The LOD for hydrogen peroxide of 31 nM is sufficiently sensitive to measure hydrogen peroxide in rain and other sources of water, for example, where the presence of hydrogen peroxide is indicative of pollution.

(78) Materials. All reactions were performed in flame-dried glassware under a positive pressure of argon unless otherwise noted. Air- and moisture-sensitive liquids were transferred via syringe or stainless steel cannula. Organic solutions were concentrated by rotary evaporation (25-40 mmHg) at 30 C. All reagents were purchased commercially and were used as received unless otherwise noted. 4-Nitrophenyl isocyanate was recrystallized from petroleum ether prior to use. N,N-Dimethylformamide, dimethylsulfoxide, tetrahydrofuran, and triethylamine were purified by known method. Flash-column chromatography was performed by known methods, employing silica gel (60 pore size, 32-63 m, standard grade). Thin-layer chromatography was carried out on silica gel TLC plates (2020 cm w/h, F-254, 250 m). Deionized water was purified by filtration and irradiation with UV light. The papers used were Whatman Chromatography Paper Grade I and Boise Aspen 30 Printer Paper (92 brilliant, 30% postconsumer content), and the tape was Ace Hardware Plastic carpet tape (part #50106).

(79) Methods. Proton nuclear magnetic resonance (.sup.1H NMR) spectra and carbon nuclear magnetic resonance spectra (.sup.13C NMR) were recorded using either a 300 MHz, 360 MHz, or 400 MHz NMR spectrometer at 25 C. Proton chemical shifts are expressed in parts per million (ppm) and are referenced to residual protium in the NMR solvent (CHCl.sub.3 7.26 ppm, CO(CH.sub.3).sub.2 2.05 ppm, or SO(CH.sub.3).sub.2 2.50 ppm). Data are represented as follows: chemical shift, multiplicity (s=singlet, bs=broad singlet, d=doublet, t=triplet, m=multiplet and/or multiple resonances), integration, and coupling constant (J) in Hertz. Carbon chemical shifts are expressed in parts per million and are referenced to the carbon resonances of the NMR solvent (CDCl.sub.3 0 77.0 ppm or CO(CH.sub.3).sub.2 29.8 and 206.3 ppm). UV/vis spectroscopic data were obtained using a six-cell spectrometer. Low resolution and high resolution mass spectra were acquired using mobile phases containing 5 mM ammonium formate. GPC data were acquired on a 3007.5 mm, 3-100 m particle size styrene divinyl benzene copolymer column using 1 mL/min N,N-dimethylformamide as the mobile phase. Molecular weights were calculated from low-angle and right-angle light scattering data. The system was calibrated using polystyrene standards.

(80) Preparation of Compound 2. Triethylamine (52 L, 0.38 mmol, 2.0 equiv) was added dropwise to a solution of 4-(hydroxymethyl)-3-methoxyphenylboronic acid pinacol ester (50 mg, 0.19 mmol, 1.2 equiv) and 4-nitrophenyl isocyanate (26 mg, 0.16 mmol, 1.0 equiv) in tetrahydrofuran (2.0 mL). The reaction mixture was stirred at 23 C. for 4 h. The solvent was removed by rotary evaporation and the residue was purified by silica gel flash column chromatography (10% ethyl acetate in hexanes, increasing to 20% ethyl acetate in hexanes) to afford compound 2 as a white, amorphous solid (46 mg, 0.11 mmol, 67%): IR (cm.sup.1) 3313, 2977, 2360, 1738, 1600, 1549, 1508; .sup.1H NMR (360 MHz, CO(CH.sub.3).sub.2) 9.46 (bs, 1H), 8.22 (d, 2H, J=9.3 Hz), 7.82 (d, 2H, J=9.3 Hz), 7.41 (d, 1H, J=7.3 Hz), 7.36 (d, 1H, J=7.4 Hz), 7.31 (s, 1H), 5.26 (s, 2H), 3.89 (s, 3H), 1.34 (s, 12H); .sup.13C NMR (360 MHz, CO(CH.sub.3).sub.2) 157.6, 154.0, 146.4, 143.4, 129.3, 128.4, 127.8, 125.7, 118.6, 116.4, 84.6, 62.9, 55.8, 25.2 (overlapping peaks in the aromatic region of the .sup.13C spectrum); MS (TOF MS AP) 427.2 (M-H.sup.+); HRMS (TOF MS AP) calcd for C.sub.21H.sub.24N.sub.2O.sub.7B (M-H.sup.+) 427.1677, found 427.1657.

(81) Preparation of Compound 8. p-Toluenesulfonic acid monohydrate (0.35 g, 1.9 mmol, 0.30 equiv) was added in one portion to a solution of compound 7 (2.4 g, 6.2 mmol, 1.0 equiv) in 4:1 tetrahydrofuran-water (62 mL) under an atmosphere of air. The reaction mixture was stirred at 23 C. for 4 h. Ethyl acetate (50 mL) and saturated aqueous sodium bicarbonate (10 mL) were added, each in one portion, and the layers were separated. The organic layer was washed with saturated aqueous sodium bicarbonate solution (150 mL) and was dried over sodium sulfate. The sodium sulfate was removed by filtration, the solvent was removed by rotary evaporation, and the residue was purified by silica gel flash column chromatography (20% ethyl acetate in hexanes, increasing to 60% ethyl acetate in hexanes) to afford compound 8 as a white, amorphous solid (1.5 g, 5.4 mmol, 87%): IR (cm.sup.1) 3540, 3470, 3269, 2963, 1727, 1615, 1547; .sup.1H NMR (400 MHz, CDCl.sub.3) 7.41-7.16 (m, 8H), 6.74 (d, 1H, J=Hz), 4.64 (s, 2H), 3.81 (s, 3H), 2.43 (bs, 1H); .sup.13C NMR (300 MHz, CDCl.sub.3) 158.0, 151.8, 150.4, 138.4, 129.4, 129.1, 125.8, 124.3, 121.6, 110.2, 101.6, 61.5, 55.3; MS (Q MS APCI+) 256.1 (MOH.sup.); HRMS (TOF MS AP+) calcd for C.sub.15H.sub.14NO.sub.3 (MOH.sup.) 256.0974, found 256.0967.

(82) Preparation of Oligomer 5. Compound 8 (0.60 g, 2.2 mmol, 1.0 equiv) was added in one portion to stirring dimethylsulfoxide (2.2 mL) at 110 C. Dibutyltin dilaurate (0.26 mL, 0.44 mmol, 0.2 equiv) was added in one portion and the reaction mixture was stirred for 2.75 min at 110 C. 4-(Hydroxymethyl)-3-methoxyphenylboronic acid pinacol ester (2.0 g, 7.6 mmol, 3.5 equiv) was added in one portion and the reaction mixture was stirred for 2 h at 110 C. The reaction mixture was cooled to 23 C. and poured into 0 C. methanol (20 mL). A yellow precipitate formed that was washed using a solid phase washing vessel by adding methanol, bubbling N.sub.2 through the solution at a vigorous rate for 15 min, then draining the solvent. This process was repeated three times. The solids were dried under vacuum for 12 h to give oligomer 9 as an off-white powder (0.17 g, 0.15 mmol, 33%); .sup.1H NMR (360 MHz, SO(CH.sub.3).sub.2) 9.90 (bs, 1H), 9.80 (bs, 3H), 9.65 (bs, 1H), 7.29-7.19 (m, 12H), 7.00-6.98 (m, 6H), 5.15 (s, 2H), 5.04 (s, 8H), 4.84 (bs, 1H), 4.39 (s, 2H), 3.84-3.70 (m, 18H), 1.29 (s, 12H). GPC M.sub.n=1.2 kDa, M.sub.w=1.7 kDa, PDI=1.44.

(83) Triethylamine (0.12 mL, 0.86 mmol, 10 equiv) was added dropwise to a solution of oligomer 9 (0.10 g, 86 mol, 1.0 equiv) and 4-nitrophenyl isocyanate (71 mg, 0.43 mmol, 5.0 equiv) in dimethylformamide (1.7 mL). The reaction mixture was stirred for 16 h at 23 C., after which the solvent was removed by rotary evaporation. The residue was washed using a solid phase washing vessel with methanol (3) followed by acetonitrile (2). The solids were dried under vacuum for 12 h to give oligomer 5 as a peach-colored powder (0.81 g, 61 mol, 71%); .sup.1H NMR (360 MHz, SO(CH.sub.3).sub.2) 10.45 (bs, 1H), 9.90 (bs, 1H), 9.80 (bs, 4H), 8.19 (d, 2H, J=9.3 Hz), 7.68 (d, 2H, J=9.1 Hz), 7.40-7.21 (m, 12H), 6.99 (d, 6H, J=8.3 Hz), 5.15 (s, 2H), 5.09-5.03 (m, 10H), 3.83-3.75 (m, 18H), 1.29 (s, 12H). GPC M.sub.n=1.4 kDa, M.sub.w=1.7 kDa, PDI=1.3.

(84) Preparation of Oligomer 6. Compound 8 (0.60 g, 2.2 mmol, 1.0 equiv) was added in one portion to stirring dimethylsulfoxide (2.2 mL) at 110 C. Dibutyltin dilaurate (0.26 mL, 0.44 mmol, 0.2 equiv) was added in one portion and the reaction mixture was stirred for 5.0 min at 110 C. 4-(Hydroxymethyl)-3-methoxyphenylboronic acid pinacol ester (2.0 g, 7.6 mmol, 3.5 equiv) was added in one portion and the reaction mixture was stirred for 2 h at 110 C. The reaction mixture was cooled to 23 C. and poured into 0 C. methanol (20 mL). A yellow precipitate formed that was washed using a solid phase washing vessel with methanol (3). The solids were dried under vacuum for 12 h to give oligomer 10 as a light yellow powder (0.30 g, 0.18 mmol, 64%); .sup.1H NMR (400 MHz, SO(CH.sub.3).sub.2) 9.89 (bs, 1H) 9.78 (bs, 6H), 9.63 (bs, 1H), 7.41-7.19 (m, 18H), 7.03-6.95 (m, 9H), 5.16 (s, 2H), 5.04 (s, 14H), 4.82 (t, 1H, J=5.6 Hz), 4.40 (d, 2H, J=5.7 Hz), 3.94-3.67 (m, 27H), 1.30 (s, 12H). GPC M.sub.n=1.3 kDa, M.sub.w=2.3 kDa, PDI=1.73.

(85) Triethylamine (0.16 mL, 1.2 mmol, 10 equiv) was added dropwise to a solution of oligomer 10 (0.20 g, 0.12 mmol, 1.0 equiv) and 4-nitrophenyl isocyanate (97 mg, 0.59 mmol, 5.0 equiv) in dimethylformamide (2.4 mL). The reaction mixture was stirred for 16 h at 23 C., after which the solvent was removed by rotary evaporation. The residue was washed using a solid phase washing vessel with methanol (3) followed by acetonitrile (2). The solids were dried under vacuum for 12 h to give oligomer 6 as a peach-colored powder (0.19 g, 0.11 mmol, 92%); .sup.1H NMR (360 MHz, SO(CH.sub.3).sub.2) 10.46 (bs, 1H), 9.91 (bs, 1H) 9.80 (bs, 6H), 8.20 (d, 2H, J=9.1 Hz), 7.69 (d, 2H, J=9.2 Hz), 7.38-7.22 (m, 18H), 7.01-6.99 (m, 9H), 5.16 (s, 2H), 5.09-5.04 (m, 16H), 3.85-3.76 (m, 27H), 1.30 (s, 12H). GPC M.sub.n=1.7 kDa, M.sub.w=1.9 kDa, PDI=1.2.

(86) Procedure for fabricating the paper-based microfluidic device. The paper was patterned using a wax printer. The wax was melted into the paper by placing the patterned paper in an oven at 150 C. for 105 s. The devices were assembled; the layout of the device is shown in FIG. 16. Specifically, FIG. 16 shows an expanded view of the device shown in FIG. 12. The device is 10 mm wide10 mm long0.9 mm thick. The paper used for all layers of wax-patterned paper in the devices was Boise Aspen 30 Printer Paper (92 brilliant, 30% postconsumer content). Layer 2 contains a paper disk (2.5 mm-diameter180 m thick) soaked in HEPES buffer (45 mM, pH 8.0) and dried. Disks were loaded with HEPES by adding 600 L of HEPES buffer to 320 mg of disks and dried under vacuum. Disks were Whatman Chromatography Paper Grade I paper fabricated using an Epilog Mini 24 Laser (CO.sub.2 laser). Layers 3 and 5 were loaded with 0.25 L of one of the hydrophobic detection reagents (e.g., compounds 1-6) dissolved in EtOAc (although compounds 5 and 6 were dissolved in THF). A solution of a hydrophobic detection reagent was spotted using a Drummond 0.25 L disposable micropipette. Layer 6 contains a paper disk soaked in green dye. The disks were loaded with the green dye by adding 600 L of green food coloring (1:5 food coloring-deionized-water) to 300 mg of disks followed by drying the disks under vacuum. After assembly, the devices were pressed using a rolling pin, applying medium pressure.

(87) Procedure for measuring flow-through. The time required for a sample to flow through the device in FIG. 16 was measured as follows: to layer 1 was added 8 L of an aqueous solution of H.sub.2O.sub.2. A timer was started immediately upon addition of the sample to the device. The device was turned over so that layer 7 was visible. The flow-through time was recorded when the hydrophilic region of layer 7 had completely changed color. Fourteen replicate tests were performed for each concentration of H.sub.2O.sub.2 and the two highest and two lowest flow-through times were removed from the data set to account for errors arising from failures during the device fabrication procedure.

(88) Measuring the depolymerization kinetics were done as follows; p-Dioxanes (250 L), dimethylsulfoxide (190 L), and phosphate buffered water (40 L, 0.01 M, pH 7.1) were added to a 2 mL vial and mixed by swirling the solution. A solution containing the oligomer (10 L from a 0.01 M solution in DMSO) was added to the vial and vortexed for 5 s. Hydrogen peroxide (10 L from a 0.2 M solution in phosphate buffered water, 0.01 M, pH 7.1) was added and the combined solution was aspirated using a pipet. The solution was transferred to a quartz cuvette (500 L, 0.1 cm path length) and the absorbance value at 385 nm was monitored continuously. Half-lives were calculated based on the relative quantity of released p-nitroaniline.

Example 3. Multiplexed Assays for Quantitative Assessment of Active Enzymes

(89) Quantitative assays for active enzymes can be conducted by measuring the time required for a reference region on a paper-based microfluidic device to turn green relative to an assay region. The intensity of the green color is not indicative of the quantity of the analyte; rather, the quantity of the analyte is directly related to the relative time required for the green color to appear. The assay is capable of measuring enzyme analytes in the picomolar range with assay times that range from 30 s to 12 min. The reference region in the device accounts for the effects of temperature, humidity, and sample viscosity on distribution rates within the device, and the assay provides quantitative results independent of the volume of the analyte (so long as a minimum volume is applied to the assay platform; e.g., 30 L). The assay platform also includes preprocessing steps to remove contaminants that may be present in a sample that could interfere with the quantitative assay. Moreover, the platform can be configured to conduct multiple quantitative assays simultaneously while requiring that a user add a drop of a test fluid to the device, then measure the time between when the reference region and the assay region turn green.

(90) FIGS. 17A-17D depict an exemplary paper-based microfluidic device for measuring active enzyme analytes quantitatively by measuring the relative time required for a sample to turn a reference region green relative to when an assay region turns green. FIG. 17A shows a photograph of the device. The device is made from stacked layers of wax-patterned paper that are held together using spray adhesive and then laminated. The dimensions of the device are 20 mm10 mm1.8 mm. The white dotted line shows the location of the cross-section depicted in FIG. 17B. The left channel in FIG. 17B is the assay region and the right channel is the reference region. FIG. 17C shows specific substrate reagents are incorporated into the device to provide selective detection of the target enzyme analyte. FIG. 17D shows hydrophobic oligomer (e.g., oligomer 4) that amplifies signal for the detection event. Amplification arises from head-to-tail depolymerization.

(91) This device has an entry point for addition of the sample, and hydrophilic channels of paper that split the sample into two equal directions (FIG. 17B). Layer 4 is where the sample splits into two directions, and where the sample encounters buffer salts to control the pH of the sample. In the left-hand channel in FIG. 17B, the sample re-dissolves a substrate for a target enzyme analyte (substrate 1, FIG. 17C), beginning in layer 5. If the target enzyme analyte is in the sample, it reacts with this substrate and causes release of one molecule of glucose per enzymatic reaction. After the sample continues through layers 5 and 6 and into layer 7, it encounters bead-bound glucose oxidase (FIGS. 17B-17C), which remains immobilized in the fibers of the paper. The glucose oxidase converts the released glucose into hydrogen peroxide as the sample wicks laterally in layer 7 of the device. Once the sample reaches the vertical conduit on the far left side of the device in FIG. 17B, it encounters an oligomer (e.g., oligomer 4 (n=2), FIG. 17D and FIG. 14B) that is hydrophobic, and thus alters the wetting properties of the paper from hydrophilic to hydrophobic (the synthesis of oligomer 4 is shown in Scheme 2, product is 51% overall yield; only 39.5 g used per device). In the absence of hydrogen peroxide, the sample wicks slowly through this hydrophobic region, but in the presence of hydrogen peroxide, oligomer 4 converts to hydrophilic products through a cascade depolymerization reaction, thus switching the wetting properties of the paper from hydrophobic back to hydrophilic. This switching reaction amplifies the effects of hydrogen peroxide on the flow rate through layers 5 and 6 by converting a large, hydrophobic oligomer (rather than a small molecule) into hydrophilic products. This switching reaction also allows the sample to pass through the layers containing oligomer 4 with a rate that depends on the concentration of hydrogen peroxide in the sample, which ultimately reflects the concentration of the target enzyme analyte. Once the sample passes through the layers containing oligomer 4, it continues to wick in the vertical direction until it re-dissolves dried green food coloring and carries the highly colored solution to the top layer where the bright green color becomes visible.

(92) The reference channel (right direction in layer 4 in the cross-section in FIG. 17B) contains the same reagents in the same order as the left-hand channel, with the exception of bead-bound glucose oxidase. In this channel, the enzyme analyte (if present) will react with the substrate deposited into the channel and generate glucose, but the glucose will not be converted into hydrogen peroxide, therefore hydrogen peroxide will not be present to react with oligomer 4. Consequently, the time required for the sample to pass through this reference region (and carry the green color to the viewing region on the top of the device) depends entirely on the temperature and humidity under which the assay is conducted, as well as on the viscosity of the sample. These factors will affect wicking rates in the assay region as well (the left-hand channel); therefore this reference region normalizes the output of the assay for the effects of these variables on sample distribution. The reference region normalizes the output of the assay since the readout is the time required for the reference region to turn green relative to when the assay region (the left-hand region) turns green.

(93) ##STR00011##

(94) The performance of the assay is demonstrated by the calibration curves shown in FIGS. 18A and 18B for the model enzyme analytes alkaline phosphatase and -D-galactosidase. In FIG. 18A, the analyte is alkaline phosphatase, which uses substrate 1a as the substrate in the device. The calibration curve was generated by depositing samples of alkaline phosphatase in 40 mM HEPES buffer (pH 8.0) to the top of the device and measuring the time (T.sub.assay) for the reference region to turn green after the assay region turns green. The limit of detection for this assay is approximately 320 pM (0.355 U/L) alkaline phosphatase, with a dynamic range of approximately 148 pM (0.164 U/L) to approximately 14.8 nM (16.4 U/L). This level of sensitivity exceeds the sensitivity of colorimetric assays that use camera-equipped cellular phones for quantification, and is more sensitive than comparable activity-based assays that use a glucose meter to obtain the quantitative readout.

(95) The calibration curves for FIG. 18A (alkaline phosphatase) and FIG. 18B (-D-galactosidase) were obtained at 19 C. and 20% relative humidity. The data points are the average of three measurements and the error bars reflect the standard deviations of these averages. The equation for the line in (a) is y=0.591x+0.349, and the equation for the line in (b) is y=0.122x+0.436. The data for alkaline phosphatase is black, catalase is green, and -D-galactosidase is blue. The assay is selective for alkaline phosphatase, as revealed by the negligible response when catalase or -D-galactosidase are added to the device instead of alkaline phosphatase (green and blue data in FIG. 18A, respectively). Catalase was used for comparison to alkaline phosphatase since it decomposes hydrogen peroxide rapidly to water and oxygen, and thus should not provide a measurable response if the mechanism of the quantitative assay relies on analyte-induced production of hydrogen peroxide to allow flow through, as expected. -D-Galactosidase was chosen because it belongs to a different enzyme family than alkaline phosphatase, and therefore demonstrates that selectivity between classes can be achieved by appropriate designs of substrate 1.

(96) If the substrate in the device is switched to detect an enzyme other than alkaline phosphatase, then the selectivity switches as well (FIG. 18B). The calibration curve in FIG. 18B is for the enzyme -D-galactosidase, which uses substrate 1b (FIG. 17C) as the substrate in the device. The limit of detection for this assay is comparable to the alkaline phosphatase assay (1.94 nM; 693 U/L), with a similar dynamic range (1.94 nM (693 U/L) to 43 nM (15,360 U/L)). This second calibration curve demonstrates that the assay can be reconfigured by changing the activity-based detection reagent (e.g., substrate 1) to target a variety of enzymes.

(97) The device includes bead-bound glucose oxidase and bead-bound catalase to scavenge glucose and hydrogen peroxide that may be in a sample. The scavenging reagents are placed in the device in layers 2 and 3 (before the sample reaches the t-junction in the cross-section in FIG. 17B), and are capable of scavenging 40 mM glucose and 30 mM hydrogen peroxide when the device is challenged with a sample of alkaline phosphatase containing one or the other contaminants. The typical concentration of glucose in blood is 3.5-5.3 mM, while the highest levels of hydrogen peroxide in urine, beverages such as tea, or rain water (in a polluted environment) are 5-100 M, 100 M, and 5.4 M, respectively. The device can remove these types of interfering contaminants.

(98) The assay can provide quantitative results without requiring precise measurements of sample volume. The hydrophilic paper absorbs a fixed volume of sample, which provides sufficient control over sample volume to allow quantitative assays, so long as a minimum quantity of sample is added to the device. The minimum volume for the device shown in FIG. 17B is 30 L.

(99) FIG. 19 shows the effect of humidity and temperature on the accuracy of measuring T.sub.assay when using alkaline phosphatase as the model enzyme analyte (11 nM (12.3 U/L) for the humidity assay and 3.7 nM (4.1 U/L) for the temperature study). The data points are the average of three measurements and the error bars reflect the standard deviations from these averages. The inset in (b) depicts the linear region of the temperature vs. T.sub.assay graph. The equation for the line is y=0.3079x3.6831. It is demonstrated that the humidity of the environment has a negligible effect on the results of the quantitative assay. Variations in temperature, however, may affect the time required for a sample to flow through the device. Temperature-induced changes in sample viscosity affect wicking rates, but are likely accounted for by the reference region, whereas temperature effects on enzymatic activity are not. Instead, as depicted in the black data in FIG. 19, the assay time tracks with the activity of the target enzyme (alkaline phosphatase). The effects of assay temperature on enzymatic activity (and, ultimately assay time) can be accounted for since there are three temperature ranges (within the ranges tested) that affect the assay: (i) 5 C. to 15 C., (ii) 15 C. to 33 C., and (iii) >33 C. The enzymatic activity is equal within the first temperature range, so no adjustment is needed to the calibration curve in FIG. 18A with the exception of adding 1 min to the measured flow through times so that they match the times in the calibration curve, which were acquired at 19 C. Likewise, another plateau appears above 33 C., which requires subtraction of 4 min from the measured flow through times. Between the range of 15 C. to 33 C., however, the relative flow through times increase linearly. This linear increase is accommodated in a quantitative assay either by (i) decreasing the measured flow through time by a factor of 0.3079 (the difference in temperature when the assay was conducted versus when the calibration curve was conducted) (0.3079 is the slope of the linear region in the inset of FIG. 19) if the assay temperature is above 19 C. (the temperature for establishing the calibration curve), or (ii) by increasing the measured flow through time by the same factor if the temperature of the assay is below 19 C. By using these adjustments, the calibration curve in FIG. 18A remains functional over a wide range of temperatures.

Example 4. Multiplexed Assays for Quantitative Assessment of Heavy Metal Contaminants

(100) Materials. All reagents were purchased commercially and were used as received unless otherwise noted. All DNA sequences were purchased from Integrated DNA technologies in IDTE buffer (pH 7.5). Deionized water was purified by filtration and irradiation with UV light. The papers used were Whatman Chromatography Paper Grade 1 and Boise Aspen 30 Printer Paper (92 brilliant, 30% postconsumer content), and the adhesive used was 3M Super 77 Multipurpose Adhesive. The laminate was Protac Ultra UV (8.0 mil) with a Diytac JetMounter JM26 laminator. Devices and laminate were cut using an Epilog mini 24 CO.sub.2 laser.

(101) Sequence of DNA Strands. Sequence id DNA Strands are listed below:

(102) TABLE-US-00002 AdenosineAssay1 AdenosineA: (SEQIDNO:1) 5-biotin-AAAAAAAAAAAACCCAGGTTCTCT-3 AdenosineB: (SEQIDNO:2) 5-TCACAGGTAAGTAAAAAAAAAAAA-biotin-3 AdenosineC: (SEQIDNO:3) 5-TTTTTTACTCATCTGTGAAGAGAACCTGGGGGAGTATTGCGGAG GAAGGT-3 LeadAssay.sup.2 LeadA: (SEQIDNO:4) 5-biotin-AAAAAAAAAAAAACAGACATCTCTTCTCCGAGCCGGT CGAAATAGGTGTAG-3 LeadB: (SEQIDNO:5) 5-biotin-AAAAAAAAAAAATGTCCGATGCTACACTATrAGGAAG AGATGTCTGT-3 MercuryAssay.sup.3 MercuryA: (SEQIDNO:6) 5-TCTCAACTCGTAAAAAAAAAAAAA-biotin-3 MercuryB: (SEQIDNO:7) 5-biotin-AAAAAAAAAAAACGCATTCAGGAT-3 Reagentd: (SEQIDNO:8) 5-TTCGTGTTGTTCCTGTTTGCG-3

(103) Adenosine aptamer beads: To 500 L, of Adenosine B (25 M) was added 250 L, of Sphero streptavidin magnetic particles (1% w/v) (Spherotech, Lake Forest, Ill.) and 250 L, of buffer. The suspension was mixed for 24 h in the dark at room temperature and then collected by magnetic separation. The beads were washed three times using buffer and redissolved in 500 L, of buffer to give a 0.5% (w/v) working solution. The formed DNA sequence is referred to as Bead-Adenosine B.

(104) Assay: To 200 L, of Adenosine A (12.5 M) was added 25 L, of streptavidin glucose oxidase (1 mg/ml) (Rockland Immunochemicals Inc., Gilbertsville, Pa.) and 575 L, of 200 mM phosphate buffer (pH 7.5). The solution was mixed at room temperature for 24 h in the dark and then collected using a Pall Nanosep 100K omega centrifugal filter. The sample was washed three times with buffer and then redissolved in 300 L, of buffer to give a working solution. The formed DNA sequence is referred to as GOX-Adenosine A. To 200 L, of Bead-Adenosine B was added 100 L, of GOX-Adenosine A and 100 L, of Adenosine C. The solution was mixed at room temperature in the dark for 90 min and then collected by magnetic separation. The suspension was washed three times with 40 mM HEPES buffer (pH 8.0) and redissolved in HEPES buffer. The final volume of buffer used was varied to change the concentration of adenosine aptamer solution added to the device.

(105) Control: To 100 L, of Adenosine A (12.5 M) was added 625 L, of streptavidin (2 M). The solution was mixed at room temperature for 24 h in the dark and then collected using a Pall Nanosep 10K omega centrifugal filter. The sample was washed three times with buffer and redissolved in 300 L, of buffer to give a working solution. The formed DNA sequence is referred to as Strep-Adenosine A. To 200 L, of Bead-Adenosine B was added 100 L, of Strep-Adenosine A and 100 L, of Adenosine C. The solution was mixed at room temperature in the dark for 90 min and collected by magnetic separation. The suspension was washed three times with 40 mM HEPES buffer (pH 8.0) and redissolved in HEPES buffer. The final volume of buffer used was varied to change the concentration of adenosine aptamer solution added to the device.

(106) Lead aptamer beads: To 500 L of Lead A (25 M) was added 250 L of Sphero streptavidin magnetic particles (1% w/v) (Spherotech, Lake Forest, Ill.) and 250 L of buffer. The suspension was mixed for 24 h in the dark at room temperature and collected by magnetic separation. The beads were washed three times using buffer and redissolved in 500 L of buffer to give a 0.5% (w/v) working solution. The formed DNA sequence is referred to as Bead-Lead A.

(107) Reagent 1: To 200 L of Lead B (12.5 M) was added 25 L of streptavidin glucose oxidase (1 mg/ml) (Rockland Immunochemicals Inc., Gilbertsville, Pa.) and 575 L of 200 mM phosphate buffer (pH 7.5). The solution was mixed at room temperature for 24 h in the dark and collected using a Pall Nanosep 100K omega centrifugal filter. The sample was washed three times with buffer and redissolved in 300 L of buffer to give a working solution. The formed DNA sequence is referred to as GOX-Lead B. To 300 L of Bead-Lead A was added 300 L of GOX-Lead B. The solution was mixed at room temperature in the dark for 90 min and collected by magnetic separation. The suspension was washed three times with 40 mM HEPES buffer (pH 8.0) and redissolved in 300 L of HEPES buffer to give a 0.5% (w/v) working solution.

(108) Reagent 2: To 200 L of Lead B (12.5 M) was added 625 L of streptavidin (2 M). The solution was mixed at room temperature for 24 h in the dark and collected using a Pall Nanosep 10K omega centrifugal filter. The sample was washed three times with buffer and redissolved in 300 L of buffer to give a working solution. The formed DNA sequence is referred to as Strep-Lead B. To 300 L of Bead-Lead A was added 300 L of Strep-Lead B. The solution was mixed at room temperature in the dark for 90 min and collected by magnetic separation. The suspension was washed three times with 40 mM HEPES buffer (pH 8.0) and redissolved in 300 L of HEPES buffer to give a 0.5% (w/v) working solution.

(109) Mercury Aptamer: Reagent c: To 500 L of Mercury A (25 M) was added 250 L of Sphero streptavidin magnetic particles (1% w/v) (Spherotech, Lake Forest, Ill.) and 250 L of buffer. The suspension was mixed for 24 h in the dark at room temperature and collected by magnetic separation. The beads were washed three times using buffer and the supernatant was removed and the beads were stored for use later. The formed DNA sequence is referred to as Reagent c.

(110) Reagent 3: To 200 L, of Mercury B (12.5 M) was added 25 L, of streptavidin glucose oxidase (1 mg/ml) (Rockland Immunochemicals Inc., Gilbertsville, Pa.) and 575 L, of 200 mM phosphate buffer (pH 7.5). The solution was mixed at room temperature for 24 h in the dark and collected using a Pall Nanosep 100K omega centrifugal filter. The sample was washed three times with buffer and redissolved in 300 L, buffer to give a working solution. The formed DNA sequence is referred to as Reagent b. To the solid Reagent c was added 300 L, of Reagent b, and the solution was split into two 150 L, aliquots. To one aliquot was added 100 L, of Reagent d and 750 L, of 40 mM HEPES buffer (pH 8.0) to give a 0.1% (w/v) working solution.

(111) Reagent 4: To the second aliquot of Reagent c and reagent e was added 850 L, of 40 mM HEPES buffer (pH 8.0) to give a 0.1% (w/v) working solution.

(112) Device for Assays of a Single Analyte. Assay devices for lead(II) or mercury(II) were assembled according to the procedure in Example 3. The layout of the device is shown in FIG. 20. The paper used for all layers was Whatman Chromatography Paper Grade I. Layer 2 contained 1 L, of food coloring (1:5 food coloring: deionized-water) pre-deposited in the outer regions. The central region contained 8 L, of immobilized glucose oxidase (0.25% w/v). The procedure for preparing the immobilized glucose oxidase was performed by known methods. Layer 2 also contained 8 L, of immobilized catalase (0.25% w/v). The immobilized catalase was deposited in layer 2 and then allowed to air dry at room temperature for 45 minutes before the immobilized glucose oxidase was added and allowed to air dry. The outer two circles in layer 3 were predeposited with 0.25 L, of oligomer 5 (4 mM) in THF. The outer circles in layer 4 were predeposited with 3 L, of glucose (50 mM). Both channels in layer 5 were predeposited with 3 L, of trehalose (1 M) and dried in ambient conditions for 30 minutes. The start channel in layer 5 (left-hand channel) was then pre-deposited with 3 L, of the assay aptamer, while the stop channel (right-hand channel) was pre-deposited with 3 L, of the control aptamer. All layers were air-dried in ambient conditions for 30 minutes after the reagents were deposited.

(113) The devices were assembled as sheets (20 cm20 cm) by aligning the edges of each layer on top of each other and using 3M Super 77 Multipurpose Adhesive to adhere individual layers together. The sheets of devices were then pressed using a Drytac JetMounter JM 26 laminator with medium pressure. Using a CO.sub.2 laser (Epilog Mini 24 Laser), individual devices were cut out with tabs left between devices to allow for processing of the devices as a sheet. In a sheet of Protac Ultra UV (8.0 mil) (22 cm22 cm), holes (d=5 mm) were cut using the CO.sub.2 laser to align with individual devices in the sheet of devices. The devices were laminated between two sheets of 22 cm22 cm Protac Ultra UV (the cut sheet covering layer 1) using a Drytac JetMounter JM 26 with medium pressure. Following lamination, individual devices were cut out using scissors.

(114) The devices contained two channels (one channel leading to a start region and the other to a stop region). The device was used along with aptamers for adenosine to demonstrate that the configuration of the device and the use of aptamers provided quantitative assays based on measurements of time (FIG. 20). The sensitivity of the assay was optimized by altering the quantity of the aptamer reagents. There was a quantitative relationship between the concentration of adenosine and the time-based measurement (FIG. 21A). The quantity of the aptamer in layer 5 had a profound impact on the limit-of-detection for the assay, where higher quantities of aptamer correlated with lower limits-of-detection (FIG. 21B). The limit-of detection for adenosine can be varied from 16 mM to 5 nM, with dynamic ranges of 1000-fold for each variation in the quantity of the aptamer.

(115) Immobilization of Enzymes on Polystyrene Beads. Catalase: To 1 mL of catalase (20 mg/mL) in 100 mM phosphate-buffered saline (pH 7.4) was added 5 mg biotin-X-NHS (EMD Chemicals, San Diego, Calif.) and mixed at room temperature for 4 hours to form biotin-catalase (b-catalase). The b-catalase was purified three times using PD-10 prepacked desalting column (GE Healthcare, Buckinghamshire, UK), washing with 100 mM phosphate-buffered saline (pH 7.4) and then concentrating by lyophilization. To 4 mg of b-catalase was added 1 mL of 9 m-diameter Sphero streptavidin magnetic particles (1% w/v) (Spherotech, Lake Forest, Ill.) and the resulting solution was mixed for 3 hours. The streptavidin beads were washed four times with HEPES buffer (40 mM, pH 8.0), concentrating by centrifugation between washes and then concentrated by lyophilization. The lyophilized powder was dissolved in 2 mL of HEPES buffer to give an immobilized catalase working solution (0.25% w/v).

(116) Glucose Oxidase: To 4 mg of biotin-glucose oxidase (b-GOx) (Rockland Immunochemicals Inc., Gilbertsville, Pa.) was added 1 mL of 9 m-diameter Sphero streptavidin magnetic particles (1% w/v) (Spherotech, Lake Forest, Ill.) and the resulting solution was mixed for 3 hours. The streptavidin beads were washed four times with HEPES buffer (40 mM, pH 8.0), concentrating by centrifugation between washes and then concentrated by lyophilization. The lyophilized powder was dissolved in 2 mL of HEPES buffer to give an immobilized glucose oxidase working solution (0.25% w/v).

(117) Procedure for Performing Assays for Single Analyte. The assay time was measured as follows: to layer 1 was added 60 L of sample. When the start region turned green, a timer was started. The assay time was recorded when the stop region turned red. Six replicate tests were performed for each sample and both the highest and lowest assay times were removed from the data set to account for errors arising from failures during the device fabrication process.

(118) Data for the Assay for Adenosine

(119) Table 2 shows assay times for detecting adenosine in a single channel assay device containing 0.00625% (w/v) beads containing adenosine aptamer in layer 5. There were 6 replicates for each concentration of adenosine. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(120) TABLE-US-00003 TABLE 2 Standard Adenosine Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (nM) (s) (s) (s) (s) (s) (s) (s) (s) 0 1 7 10 20 21 28 14.5 7.1 10 6 7 12 27 27 142 18.3 10.3 25 8 10 26 26 33 42 23.8 9.7 50 48 52 68 73 113 256 76.5 25.9 100 27 141 203 204 215 190.8 33.6 250 306 315 315 322 324 333 319.0 4.7 500 521 545 583 596 631 656 588.8 35.5 1000 1225 1345 1375 1382 1391 1404 1373.3 19.9

(121) Table 3 shows assay times for detecting adenosine in a single channel assay device containing 0.05% (w/v) beads containing adenosine aptamer in layer 5. There were 6 replicates for each concentration of adenosine. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(122) TABLE-US-00004 TABLE 3 Standard Adenosine Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (nM) (s) (s) (s) (s) (s) (s) (s) (s) 0 1 1 5 22 39 2700 16.8 17.4 10 4 7 8 16 33 82 16.0 12.0 50 114 132 152 152 172 180 152.0 16.3 100 68 274 287 294 298 301 288.3 10.5 500 55 442 454 485 488 521 467.3 22.8 1000 11 665 881 889 925 1067 842.5 119.7

(123) Table 4 shows assay times for detecting adenosine in a single channel assay device containing 0.083% (w/v) beads containing adenosine aptamer in layer 5. There were 6 replicates for each concentration of adenosine. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(124) TABLE-US-00005 TABLE 4 Standard Adenosine Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (nM) (s) (s) (s) (s) (s) (s) (s) (s) 0 6 11 12 12 21 33 14.0 4.7 1 1 2 2 10 16 20 7.5 6.8 5 18 22 25 32 41 52 30.0 8.4 10 8 32 42 47 67 74 47.0 14.7 50 61 67 88 109 110 138 93.5 20.4 100 265 289 296 304 344 381 3083 24.6 1000 892 906 911 914 915 911.5 4.0

(125) Table 5 shows assay times for detecting adenosine in a single channel assay device containing 0.25% (w/v) beads containing adenosine aptamer in layer 5. There were 6 replicates for each concentration of adenosine. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(126) TABLE-US-00006 TABLE 5 Standard Adenosine Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (nM) (s) (s) (s) (s) (s) (s) (s) (s) 0 1 2 4 7 14 21 6.8 5.3 50 1 1 10 13 15 23 9.8 6.2 100 13 15 28 28 33 40 26.0 7.7 500 10 68 79 84 90 236 80.3 9.3 1000 126 132 142 143 149 158 141.5 7.0 10000 113 170 181 197 202 405 187.5 14.7 100000 28 326 431 452 524 433.3 81.8

(127) Table 6 shows assay times for detecting adenosine in a single channel assay device containing 0.5% (w/v) beads containing adenosine aptamer in layer 5. There were 6 replicates for each concentration of adenosine. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(128) TABLE-US-00007 TABLE 6 Standard Adenosine Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (nM) (s) (s) (s) (s) (s) (s) (s) (s) 0 2 2 3 10 25 630 10.0 10.6 5 2 5 12 13 15 39 11.3 4.3 10 4 10 20 50 54 58 33.5 21.8 50 12 24 55 55 59 48.3 16.3 100 62 90 101 109 123 132 105.8 13.9 500 100 136 151 163 177 156.8 17.4 1000 275 370 433 437 438 670 419.5 33.1 5000 22 450 462 463 464 459.8 6.6 10000 788 789 794 814 822 824 804.8 15.8

(129) Table 7 shows assay times for detecting adenosine in a single channel assay device containing 1.0% (w/v) beads containing adenosine aptamer in layer 5. There were 6 replicates for each concentration of adenosine. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(130) TABLE-US-00008 TABLE 7 Standard Adenosine Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (nM) (s) (s) (s) (s) (s) (s) (s) (s) 0 9 11 11 13 14 21 12.3 1.5 1 1 2 3 21 22 26 12.0 11.0 5 11 12 45 48 52 89 39.3 18.4 10 77 86 87 116 138 138 106.8 25.1 50 127 150 151 160 172 173 158.3 10.2 100 118 182 218 244 292 306 234.0 46.3 500 539 564 575 602 603 608 586.0 19.6 1000 854 874 889 908 942 993 903.3 29.3
Effect of the Quantity of the Aptamer on the Limit-of-Detection of the Assay

(131) Table 8 shows the change in limit of detection with mass of aptamer-beads added to devices for detecting adenosine.

(132) TABLE-US-00009 TABLE 8 Aptamer-Beads (g) LOD (nM) 0.1875 15892.5 1.5 11977.0 2.49 5071.9 7.5 116.0 15 14.7 30 5.1

(133) Samples containing 100 nM adenosine that were spiked with either glucose or hydrogen peroxide were added to the device for performing assays of a single analyte (FIG. 20). Devices that did not contain immobilized glucose oxidase and immobilized catalase were used to show the effects of glucose and hydrogen peroxide on assays lacking preprocessing.

(134) Effects of Preprocessing

(135) No preprocessing; Table 9 shows assay times for detecting 100 nM adenosine spiked with glucose in a single channel assay device containing 0.5% (w/v) beads containing adenosine aptamer in layer 5. No immobilized glucose oxidase or catalase was present in layer 2. There were 6 replicates for each concentration of glucose. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(136) TABLE-US-00010 TABLE 9 Standard Glucose Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (mM) (s) (s) (s) (s) (s) (s) (s) (s) 0 109 234 247 256 257 290 248.5 10.7 10 8 141 164 192 192 172.3 24.7 25 8 62 91 96 97 86.5 16.5 50 3 19 23 34 71 220 36.8 23.7

(137) No preprocessing; Table 10 shows assay times for detecting 100 nM adenosine spiked with hydrogen peroxide in a single channel assay device containing 0.5% (w/v) beads containing adenosine aptamer in layer 5. No immobilized glucose oxidase or catalase was present in layer 2. There were 6 replicates for each concentration of hydrogen peroxide. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(138) TABLE-US-00011 TABLE 10 Hydrogen Standard Peroxide Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (mM) (s) (s) (s) (s) (s) (s) (s) (s) 0 109 234 247 256 257 290 248.5 10.7 0.5 1 2 7 8 11 16 7.0 3.7 10 2 13 36 38 43 64 32.5 13.3 25 1 1 2 3 10 24 4.0 4.1 50 5 6 26 32 32 44 24.0 12.3

(139) With preprocessing; Table 11 shows assay times for detecting 100 nM adenosine spiked with glucose in a single channel assay device containing 0.5% (w/v) beads containing adenosine aptamer in layer 5. Immobilized glucose oxidase or catalase was present in layer 2. There were 6 replicates for each concentration of glucose. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(140) TABLE-US-00012 TABLE 11 Standard Glucose Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (mM) (s) (s) (s) (s) (s) (s) (s) (s) 0 203 209 247 249 260 275 241.3 22.2 10 12 251 265 268 282 266.5 12.7 25 112 198 205 212 238 258 213.3 17.5 50 68 285 223 242 270 230 35.7

(141) With preprocessing; Table 12 shows assay times for detecting 100 nM adenosine spiked with hydrogen peroxide in a single channel assay device containing 0.5% (w/v) beads containing adenosine aptamer in layer 5. Immobilized glucose oxidase or catalase was present in layer 2. There were 6 replicates for each concentration of hydrogen peroxide. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(142) TABLE-US-00013 TABLE 12 Hydrogen Standard Peroxide Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (mM) (s) (s) (s) (s) (s) (s) (s) (s) 0 203 209 247 249 260 275 241.3 22.2 0.5 172 243 260 268 292 297 265.8 20.4 10 205 211 230 237 250 308 232.0 16.3 25 204 222 232 235 285 293 243.5 28.2 50 231 235 242 242 270 288 247.3 15.5
Assay for Pb.sup.2+

(143) Table 13 shows assay times for detecting lead(II) in a single channel assay device containing 0.5% (w/v) reagents 1 and 2 in layer 5. There were 6 replicates for each concentration of lead. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(144) TABLE-US-00014 TABLE 13 Standard Lead (II) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (mM) (s) (s) (s) (s) (s) (s) (s) (s) 0 18 20 24 32 63 102 34.8 19.5 1 15 22 28 29 30 37 27.3 3.6 5 1 43 47 54 60 60 51.0 7.5 10 24 69 75 88 94 187 81.5 27.6 25 84 100 126 134 167 248 131.8 27.6 50 29 120 128 140 142 145 132.5 10.4 100 189 203 210 211 217 331 210.3 5.7 500 300 336 341 343 345 390 341.3 3.9 1000 40 300 357 377 395 439 357.3 41.2 100000 668 675 679 757 772 781 720.75 50.9
Assay for Hg.sup.2+

(145) Table 14 shows assay times for detecting mercury(II) in a single channel assay device containing 0.1% (w/v) reagents 3 and 4 in layer 5. There were 6 replicates for each concentration of mercury. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(146) TABLE-US-00015 TABLE 14 Mercury Standard (II) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Deviation (mM) (s) (s) (s) (s) (s) (s) (s) (s) 0 1 12 18 23 50 70 25.8 16.8 1 13 16 50 72 96 519 58.5 34.0 5 89 109 132 159 163 184 140.8 25.3 10 226 232 245 262 270 252.3 17.1 25 114 297 328 332 335 345 323.0 17.6 50 114 393 417 432 434 610 419.0 18.9 100 442 524 585 593 618 619 580.0 39.9 10000 90 747 799 824 859 930 807.3 47.1

(147) Fabrication of the device for performing assays on multiple analytes simultaneously. Devices were assembled according to the procedure in Example 3; the layout is shown in FIG. 22. The paper used for all layers was Whatman Chromatography Paper Grade I. Layer 2 contained 1 L of food coloring (1:5 food coloring: deionized-water) pre-deposited in the outer regions. The central region contained 8 L of immobilized glucose oxidase (0.25% w/v). Layer 2 also contained 8 L of immobilized catalase (0.25% w/v). The immobilized catalase was deposited in layer 2 and then allowed to air dry at room temperature for 45 minutes before the immobilized glucose oxidase was added and allowed to air dry. The outer circles in layer 3 were predeposited with 0.25 L of oligomer 5 (4 mM) in THF. The outer circles in layer 4 were predeposited with 3 L of glucose (50 mM). All channels in layer 5 were predeposited with 3 L of trehalose (1 M) and dried in ambient conditions for 30 minutes. For the mercury portion of the device (top row), the start channel in layer 5 (left-hand channel) was then pre-deposited with 3 L of reagent 3, while the stop channel (right-hand channel) was pre-deposited with 3 L of reagent 4. For the lead portion of the device (top row), the start channel in layer 5 (left-hand channel) was then pre-deposited with 3 L of reagent 1, while the stop channel (right-hand channel) was pre-deposited with 3 L of reagent 2. All layers were air-dried in ambient conditions for 30 minutes after reagents were deposited.

(148) The devices were assembled as sheets (20 cm20 cm) by aligning the edges of each layer on top of each other and using 3M Super 77 Multipurpose Adhesive to adhere individual layers together. The sheets of devices were then pressed using a Drytac JetMounter JM 26 laminator with medium pressure. Using a CO.sub.2 laser (Epilog Mini 24 Laser), individual devices were cut out with tabs left between devices to allow for processing of the devices as a sheet. In a sheet of Protac Ultra UV (8.0 mil) (22 cm22 cm), holes (d=5 mm) were cut using the CO.sub.2 laser to align with individual devices in the sheet of devices. The devices were laminated between two sheets of 22 cm22 cm Protac Ultra UV (the cut sheet covering layer 1) using a Drytac JetMounter JM 26 with medium pressure. Following lamination, individual devices were cut out using scissors.

(149) Procedure for performing assays on multiple analytes simultaneously. The assay time was measured as follows: to layer 1 was added 80 L of sample. When the start region turned green, a timer was started. The assay time was recorded when the stop region turned red. Each assay (mercury and lead) was timed separately so that two times are measured, one for each analyte quantified. Six replicate tests were performed for each sample and both the highest and lowest assay times were removed from the data set to account for errors arising from failures during the device fabrication process.

(150) Use of De-Ionized Water

(151) Table 15 shows assay times for detecting mercury(II) and lead(II) in a multiple channel assay device containing 0.1% (w/v) reagents 3 and 4, and 0.5% (w/v) reagents 1 and 2 in layer 5. Samples were made using deionized water. There were 5 replicates for each sample. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(152) TABLE-US-00016 TABLE 15 Standard Trial 1 Trial Trial Trial Trial Average Deviation Heavy Metal(s) Assay (s) 2 (s) 3 (s) 4 (s) 5 (s) (s) (s) 0 nM Pb.sup.2+ and 0 nM Hg(II) 1 12 13 17 33 14.0 2.6 Hg.sup.2+ Pb(II) 2 6 9 34 45 16.3 15.4 100 nM Pb.sup.2+ and 0 nM Hg(II) 1 2 4 1 22 6.0 5.3 Hg.sup.2+ Pb(II) 189 203 220 243 244 222.0 20.1 0 nM Pb.sup.2+ and 100 nM Hg(II) 574 534 582 488 566 534.7 47.0 Hg.sup.2+ Pb(II) 1 2 21 23 30 15.3 11.6 100 nM Pb.sup.2+ and Hg(II) 504 506 541 557 580 534.7 26.1 100 nM Hg.sup.2+ Pb(II) 234 238 236 236 309 236.7 1.2 100 nM Pb.sup.2+, 100 nM Hg(II) 510 519 520 564 572 534.3 25.7 Hg.sup.2+, 100 nM Pb(II) 219 229 240 253 284 240.7 12.0 Cd.sup.2+ and 100 nM Zn.sup.2+
Use of Lake Water

(153) Samples were prepared with lake water from Tussey Lake (40 46 11.28 N, 77 45 29.88 W). The lake water was spiked with lead(II) to simulate contaminated drinking water.

(154) Table 16 shows assay times for detecting mercury(II) and lead(II) in a multiple channel assay device containing 0.1% (w/v) reagents 3 and 4, and 0.5% (w/v) reagents 1 and 2 in layer 5. Samples were made using lake water. There were 5 replicates for each sample. To account for errors in fabricating the devices, the fastest and slowest assay times were not used in determining the average or standard deviation values.

(155) TABLE-US-00017 TABLE 16 Standard Trial 1 Trial Trial Trial Trial Average Deviation Heavy Metal(s) Assay (s) 2 (s) 3 (s) 4 (s) 5 (s) (s) (s) O nM Pb.sup.2+ and 0 nM Hg(II) 1 8 9 10 22 9.0 1.0 Hg.sup.2+ Pb(II) 1 1 4 13 14 6.0 6.2 100 nM Pb2+ and Hg(II) 1 9 15 16 62 13.3 3.8 0 nM Hg.sup.2+ Pb(II) 233 241 245 246 248 244.0 2.6 10 nM Pb.sup.2+ and 0 nM Hg(II) 1 1 1 17 69 6.3 9.2 Hg.sup.2+ Pb(II) 70 80 81 83 83 81.3 1.5 1 nM Pb.sup.2+ and 0 nM Hg(II) 2 2 5 25 27 10.7 12.5 Hg.sup.2+ Pb(II) 11 30 32 52 58 38.0 12.2

(156) The disclosed assay for inorganic ions (e.g., Pb.sup.2+ and Hg.sup.2+) provides several advantages. The configuration uses aptamers and thus opens the assay strategy to new classes of analytes. The time-based approach can be used for quantitative analysis of enzymes, inorganic ions, and small molecules. The assays are easily reconfigured by exchanging reagents in the device both to alter the specificity for a target analyte, and to adjust the sensitivity and dynamic range of the assay(s). The strategy includes multiplexed assays, which allows complete analysis of samples using a single aliquot of the sample and a single step by the user. The assay is conveniently used, requiring that the user only add a sample onto the device. Pre-processing occurs within the device, and the device meters the volume of the sample to enable a quantitative readout. The assay platform includes reagents both for selective detection as well as signal amplification, yet no washing steps or other manipulations are required.

(157) FIG. 23 depicts photographs of a paper-based assay platform for simultaneous measurements of Hg.sup.2+ and Pb.sup.2+ in drinking water. The white dotted lines denote the edges of laminate that hold multiple layers of stacked, waxpatterned paper in contact with one another. The dimensions of the stacked paper are 20 mm30 mm1.8 mm (FIG. 22). The device has hydrophobic regions that define where the sample travels by capillary action through the hydrophilic regions in the paper. Typically, a few minutes after adding the sample to the center region, the start hydrophilic region turns green, at which point the user begins to measure the time required for the stop region to turn red (FIG. 23). The measurement of time, which can be accomplished in a number of ways without using an electronic timer, reflects the concentration of the analyte in the sample. The device in FIG. 23 is configured to quantify Hg.sup.2+ and Pb.sup.2+ in water simultaneously. Therefore, once the sample of water is added to the entry point, it distributes into four separate portions of the device, two of which lead to start regions, while the other two lead to complementary stop regions.

(158) The sample distributes to the start and stop regions through five layers of wax-patterned paper that are held together via laminate (FIGS. 24-26). Each layer and hydrophilic region in the device serves a unique function to facilitate the assay, with the central hydrophilic region in layer 1 operating as the collection zone where the sample is added to the device. Bead-bound glucose oxidase (GOX) and catalase in layer 2 pre-processes the sample to remove up to 50 mM glucose and hydrogen peroxide that would otherwise interfere with the assay. The beads are embedded in the fibers of the paper, therefore these enzymes should remain fixed in layer 2, whereas the sample distributes to hydrophilic regions of the device.

(159) The 5th layer is a lateral-flow region where the sample moves away from the central sample addition region to the outer columns of hydrophilic paper. In the four lateral-flow channels, the sample re-dissolves reagents for conducting the assays, where each of the four channels contains a different set of reagents (FIG. 25). Reagent 1 is used in the assay for Pb.sup.2+ and is a bead-bound aptamer complex including two polynucleotides, one of which is a DNAzyme bound to a 9 m-diameter bead that causes the complex to remain embedded in the paper. The second polynucleotide is covalently bound to GOX and non-covalently associated with the DNAzyme. When Pb.sup.2+ is present in the sample, it activates the DNAzyme, which cleaves the polynucleotide that is bound to GOX, thus releasing GOX into solution. GOX is then free to travel with the solvent to the outer hydrophilic columns, whereas the remainder of reagent 1 remains in layer 5.

(160) At the outer columns, the sample travels vertically towards the top of the paper-based device. As it passes through layers of paper, the sample encounters additional reagents that were pre-deposited and dried into the layers before the device was assembled. In layer 4, the sample re-dissolves glucose, which is processed by the liberated GOX to generate hydrogen peroxide. Layer 3 contains a hydrophobic poly(benzyl carbamate) oligomer 6 that reacts with hydrogen peroxide via the aryl boronate on one end of the oligomer to initiate a continuous head-to-tail depolymerization reaction (FIG. 26). This depolymerization process changes the wetting properties of layer 3 from hydrophobic back to hydrophilic and allows the sample to travel through this layer with a rate that depends on the concentration of hydrogen peroxide that is generated from the aptamer detection event. The 2.sup.nd layer contains dried food coloring, which, once re-dissolved, is carried to the top layer where it becomes visible in the appropriate start or stop region.

(161) The Pb.sup.2+ assay also contains reagent 2 in the lateral-flow channel opposite to reagent 1. Reagent 2 is exactly the same as reagent 1, with the exception that GOX is replaced with streptavidin. Streptavidin will not generate hydrogen peroxide when it encounters glucose, therefore a sample containing Pb.sup.2+ will take longer to travel to the viewing region when it encounters reagent 2 than when it encounters reagent 1. This difference in sample transport time may be dependent on the concentration of Pb.sup.2+ in the sample, thus providing the basis for the time-based measurement and quantitative assay. Moreover, this type of measurement, based on relative sample transport time, normalizes the assays for effects of humidity and sample viscosity that would normally complicate a quantitative measurement.

(162) The Hg.sup.2+ assay occurs on the same device simultaneously with the Pb.sup.2+ assay, but requires a different set of reagents in layer 5 than the Pb.sup.2+ assay (e.g., reagents 3 and 4, FIG. 25). Reagent 4 includes three components: polynucleotide b bound to GOX, polynucleotide c bound to a 9 m-diameter bead to affix c to the paper, and polynucleotide d, which brings b, c, d, and Hg.sup.2+ together into a four-component non-covalent complex when Hg.sup.2+ is present in the sample. This complex prevents the GOX from traveling with the sample through the remainder of the device, and thus minimizes the quantity of hydrogen peroxide that is generated in layer 4 to cause depolymerization in layer 3.

(163) Reagent 3 in the opposite lateral-flow region to reagent 4 contains only polynucleotides b and c, and thus is incapable of forming the four-component complex in the presence of Hg.sup.2+. In this channel, all of the GOX is capable of generating hydrogen peroxide in layer 4. Hence, the sample travels faster through the region containing reagent 3 than reagent 4, thus reagent 3 leads to the start region and reagent 4 to the stop region.

(164) Using a device that contains only two channels rather than four (e.g., one channel leading to a start region and the other to a stop region; FIG. 20), dose-dependent responses were established for Pb.sup.2+ and Hg.sup.2+ (FIGS. 28A-D). The limit-of-detection for the Hg.sup.2+ assay is approximately 4 nM (1 ppb) and the dynamic range extends up to approximately 25 nM (7 ppb), while the complimentary numbers for the Pb.sup.2+ assay are approximately 4 nM (1 ppb) up to approximately 50 nM (17 ppb). Both of these assays may be sufficiently sensitive to measure the World Health Organization's recommended upper limits for these ions in drinking water (e.g., 22 nM (6 ppb) for Hg.sup.2+ and 30 nM (10 ppb) for Pb.sup.2+).

(165) Based on these results in two-channel devices, the four-channel design was implemented for ion analysis in drinking water, as depicted in FIG. 23. The assay times obtained in the four-channel devices are identical to those obtained in the two-channel versions, indicating that the calibration curves in FIG. 28 can be applied to the aforementioned four-channel device. Moreover, FIG. 29 reveals that the multiplexed assays in the four-channel devices are selective for the desired analytes. For example, assay times for samples containing either 100 nM Pb.sup.2+; 100 nM of Pb.sup.2+ and Hg.sup.2+; or 100 nM of Pb.sup.2+, Hg.sup.2+, Cd.sup.2+, and Zn.sup.2+, all gave identical measurement times for Pb.sup.2+. Likewise, the measurement time for a sample containing 100 nM Hg.sup.2+ was identical to times obtained using samples containing Pb.sup.2+ or a combination of Pb.sup.2+, Hg.sup.2+, Cd.sup.2+, and Zn.sup.2+.

(166) Furthermore, the assay platform provides consistent, quantitative measurements even when lake water is used rather than de-ionized water (DI). For example, this observation is shown in FIG. 29 for lake water that was spiked with three different concentrations of Pb.sup.2+. Comparison of measurement times between DI water and lake water for 100 nM Pb.sup.2+, in particular, reveals that the assay is minimally affected by contaminants in lake water.