DISPOSABLE BIOREACTOR SYSTEM

Abstract

A cylindrical or annular polymeric bioreactor is disclosed which provides enhanced mixing and aeration of the growth medium while simultaneously offering reduced mechanical shear force.

Claims

1-15. (canceled)

16. An apparatus comprising: (a) a hollow toroidal one-piece polymeric bioreactor vessel comprising at least one port configured to receive at least one pre-inserted and pre-calibrated sensor to monitor a bioprocess carried out in said hollow toroidal one-piece polymeric bioreactor vessel; and (b) a drive configured to move the hollow toroidal one-piece polymeric bioreactor vessel such that there is build-up of a resonant frequency traveling wave of fluid orbiting in one direction in an interior of the hollow toroidal one-piece polymeric bioreactor vessel when a particular amplitude and orbital speed is imparted to said hollow toroidal one-piece polymeric bioreactor vessel, wherein the resonant frequency depends on size and shape of the hollow toroidal one-piece polymeric bioreactor vessel, and wherein the hollow toroidal one-piece polymeric bioreactor vessel was sterilized by gamma radiation.

17. The apparatus in accordance with claim 16, wherein the hollow toroidal one-piece polymeric bioreactor vessel comprises a textured surface on an inner surface of a bottom of the hollow toroidal one-piece polymeric bioreactor vessel.

18. The apparatus in accordance with claim 17, wherein the textured surface comprises a plurality of upstanding protuberances and said hollow toroidal one-piece polymeric bioreactor vessel includes at least one exit port for removal of gaseous by-products.

19. The apparatus in accordance with claim 18, wherein at least some of said upstanding protuberances allow passage of sparging gas into said hollow toroidal one-piece polymeric bioreactor vessel.

20. The apparatus in accordance with claim 16, further comprising pre-molded baffles on at least a portion of an inner surface of said hollow toroidal one-piece polymeric bioreactor vessel.

21. The apparatus in accordance with claim 16, wherein the hollow toroidal one-piece polymeric bioreactor vessel is fabricated from a substantially rigid plastic.

22. The apparatus in accordance with claim 16, wherein the hollow toroidal one-piece polymeric bioreactor vessel is fabricated from a substantially flexible plastic.

23. The apparatus in accordance with claim 16, further comprising means for illuminating contents of said hollow toroidal one-piece polymeric bioreactor vessel.

24. The apparatus in accordance with claim 23, wherein said means for illuminating the contents of said hollow toroidal one-piece polymeric bioreactor vessel comprises at least one LED.

25. The apparatus in accordance with claim 16, wherein the drive is further configured for simultaneously both orbiting and rocking said hollow toroidal one-piece polymeric bioreactor vessel.

26. The apparatus in accordance with claim 16, further comprising a plurality of input ports for inserting a sensor or process feedstock into the hollow toroidal one-piece polymeric bioreactor vessel.

27. The apparatus in accordance with claim 16, further comprising at least one pre-inserted and pre-calibrated sensor.

28. The apparatus in accordance with claim 16, wherein the drive is configured to move the hollow toroidal one-piece polymeric bioreactor vessel in a reciprocating motion to cause the fluid to orbit in a reversed direction.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0016] FIGS. 1 shows the prior art type of disposable bioreactor system (e.g., Hyclone/Thermo-Fisher) that utilizes a disposable polymeric liner and provides structural support for the liner.

[0017] FIG. 2 shows a prior art pillow disposable bioreactor bag of flexible polymeric material.

[0018] FIG. 3a shows a three-dimensional view of a bioreactor in accordance with the present invention having vertical side walls and which is toroidal in shape.

[0019] FIG. 3b shows two-dimensional views of a toroidal bioreactor in accordance with the present invention as depicted in FIG. 3a and shows alternative embodiments of the vessel having either a circular (12) or rectangular (11) cross-section.

[0020] FIG. 3c shows how the bioreactor of the present invention achieves its enhanced mixing via orbital motion (in this case moving counter clockwise indicated as 16) which creates a travelling wave of the growth medium present in the bioreactor vessel (17).

[0021] FIG. 4 shows a view of the walls of the preferred embodiment of the bioreactor vessel of FIGS. 3a and 3b where sparging is accomplished through the walls of the vessel. The walls are shown as corrugated in order to enhance mixing.

[0022] FIG. 5 shows a view of an alternative configuration for a bioreactor vessel in accordance with the present invention where the bioreactor is toroidal but oval in cross-section rather than circular. Again, the vessel can have a cross-section that is either substantially rectangular (5.2) or circular (5.3).

[0023] FIG. 6 shows an embodiment of the present invention which provides for multi-directional motion (i.e., both orbital and rocking) of the bioreactor vessel of the present invention shown in FIG. 3a, with the X and Y axes shown.

DESCRIPTION OF THE INVENTION

[0024] The most desirable single-use bioreactor solution would be one that effectively meets criteria 1 through 3, as set forth above, while at the same time requiring the fewest moving parts. Minimizing the number of moving parts will also tend to yield the least expensive and most reliable overall solution. A novel design for a polymeric bioreactor vessel in accordance with the present invention which uses a minimal number of parts is shown in FIGS. 3 through 6.

[0025] In FIG. 3a, a cylindrical bioreactor vessel 1 having a substantially rectangular cross-section is shown having a hollow core 6, so that the bioreactor is a toroid. Although the design shown in FIG. 3a is shown as having substantially vertical walls, this is not an essential aspect of the invention and a toroidal (donut) shaped vessel not having vertical surfaces is also suitable, as shown in FIG. 3b in cross section as 12. Alternatively, only one wall (either inner or outer) may be vertical and the other curved.

[0026] The vessel can suitably be of a rigid biocompatible polymer. Alternatively, the vessel can be fabricated in whole or in part of a flexible (non-rigid) polymer since the hydrostatic pressure of the liquid reaction (growth) medium present within the vessel together with the pressure of the sparging oxygen or oxygen containing gas will enable even a bag made of non-rigid polymer to substantially retain its annular shape. Suitable rigid polymers include, but are not limited to; USP Class VI approved polycarbonate and polystyrene. Suitable flexible polymers include, but are not limited to, low density polyethylene and ethylene /vinyl acetate copolymer.

[0027] In FIG. 3a, element 2 is a platform equipped with an electric motor or other drive means (not shown) which moves in an orbital motion as indicated by arrow 3 and as further shown in FIG. 3c. This orbital motion, combined with the toroidal shape of the bioreactor, sets up a traveling wave in the bioreactor growth medium. This traveling wave provides both effective mixing and oxygenation of the contents. It should be noted that in the case of a torioidal vessel whether having vertical or curved walls, the liquid motion will follow a pure traveling wave. In both cases, the exact liquid motion can be modeled using computational fluid dynamics.

[0028] Additionally, single-use bioreactor vessel 1 can suitably be equipped with one or a plurality of input ports (one shown as 4 in FIG. 3a) for process feedstock inputs (e.g.: pH buffers, glucose etc.), and/or also for pre-inserted and pre-calibrated sensors (e.g., to measure dissolved oxygen, pH, dissolved CO.sub.2 and the like). These sensors can be either traditional electrochemical sensors and/or disposable and pre-calibrated optical sensors. It is generally more efficacious to have the sensors pre-calibrated in order to avoid breaking the sterile barrier on the bioreactor. Optical probes are available that can be both pre-calibrated and can be gamma irradiated while mounted in the bioreactor. The ability to use gamma radiation allows the pre-calibrated sensors to be inserted in the bioreactor and the entire assembly subsequently sterilized. Port 5 denotes the exit port for removal of gaseous reaction by-products. In FIG. 3a 7 denotes a light source such as a bank of visible or UV light emitting LEDs that can be utilized to promote the growth of plant or algae culture or transiently activate certain genes for multi-drug production from a single cell platform The lighting is shown on the top, but this is not a necessity; the illumination can additionally or alternatively come from the sides and/or even the bottom of the vessel provided only that the growth media receives enough illumination to enhance photosynthesis or gene activation depending on the purpose of the growth run and the cell being grown. Ideally the LEDs will have a spectrum that is matched to the absorption spectrum of the species being grown. LEDs are now available from the ultra-violet through the near infrared (e.g.: http://www.marktechopto.com/) so matching to the absorption spectrum of the species under study can be easily done. Additionally, the surface of the bioreactor on which the LED's are mounted needs to be sufficiently transmitting in this region to allow the light to pass through to the interior of the bioreactor

[0029] The bioreactor vessel walls (outer and optional inner surfaces) define a structure that will have a resonant frequency which is determined by the particular configuration (size and shape) of the bioreactor. The resonant frequency of the bioreactor can be readily calculated knowing that the traveling wave must reproduce itself in phase every round trip (Hydrodynamics, Horace Lamb and Russ Caflisch, First Cambridge University Press, 1997). Once this frequency has been determined the bioreactor can be rotated with this circular frequency to thereby set-up resonant traveling wave motion of the fluid (growth medium) inside. The wave amplitude will be chosen depending on the level of mixing and agitation needed.

[0030] In FIG. 3b the top view, 10, of the bioreactor is shown along with the square or alternatively circular cross sections 11 and 12 respectively.

[0031] It is possible to further enhance the mixing and sparging efficiency of the bioreactor vessel of the present invention shown in FIG. 4 by providing a textured surface on the inner floor (bottom) and/or wall surfaces of the vessel (i.e., a surface which is in contact with the growth medium). This texturing can be achieved using baffles, ridges, bumps and/or other upstanding protuberances as shown in FIG. 4, which are preferably pre-molded on all or a part of the inner surface of the vessel. The sparging of the reaction medium is preferably accomplished in this design by bringing the oxygen or air into the bioreactor through a multitude of orifices in the floor and/or walls of the vessel. These orifices can be in the protuberances in the texturing or separate from them. In FIG. 4, the flow direction of the fluid in the annular vessel is indicated as 4.1, (which corresponds to arrow 3 in FIGS. 4), the textured surface on the inner wall and/or floor surface of the vessel (the textured surface being shown here for simplicity to have an approximately sinusoidal shape pattern) is indicated as 4.2, and the sparge orifices are indicated as 4.3.

[0032] The optimal patterning (e.g., size, shape and frequency) will be a function of the size of the reactor, the velocity, viscosity, and nature of cell platform and its associated optimized growth medium. The particular patterning which provides optimal agitation can be determined through finite element analysis studies (www.fluent.com) or through empirical experiment. These studies generally include mixing studies as a function of time or number of agitation cycles. Additional computational studies that employ Henry's law (p. 384, General Chemistry, 2.sup.nd Edition, Donald A. McQuarrie and Peter A. Rock, W. H. Freeman and Company, New York, 1987) to model oxygen transfer or calculate the oxygen transfer rate are possible. These studies require the finite element analysis code to take into account the surface area of the bubbles created during sparging. For example, a higher number of bubbles having decreased size will increase the surface area available for oxygen transfer.

[0033] In FIG. 4, the sparging gas (normally air or oxygen) is suitably brought to the bioreactor using tubing 4.3 which preferably will have a fluid diode or hydrophobic filter at or near its end that allows the sparging gas to flow into the bioreactor, but prevents the liquid (reaction medium) from draining out of the bioreactor vessel through said tubing. The aforementioned texturing of the floor and/or walls therefore does not preclude bringing the sparge gas into the vessel through the floor, top, and/or walls of the vessel. This type of sparging, combined with the traveling wave motion induced by the orbital movement of the vessel, ensures that the cells or microbes in the bioreactor have access to sufficient feed and oxygen to achieve optimal total cell density and/or maximize product yield.

[0034] It should also be noted that the motion in direction FIG. 3a is only shown as orbital, but can also involve rocking in either the X or Y axis (or both) and thereby be a two-dimensional or three-dimensional motion, which can be readily tailored to work in conjunction with the bioreactor dimensions. Such multi-directional motion is shown in FIG. 6 where 6.1 and 6.2 are the plane of the platform when rocked about axis 6.3. The platform holding the bioreactor can also be orbited in one direction according to 6.4, or agitated in both directions as per 6.5. This motion can also be a reciprocating motion where the direction of rotation of the motor which causes the vessel to orbit is periodically reversed. This periodic reversal in the direction of the vessel's orbit can be used to cause more vigorous agitation if/when required during the growth process.