Apparatus and methods for low temperature small angle X-ray scattering
09927336 · 2018-03-27
Assignee
Inventors
- Stephen P. Meisberger (Ithaca, NY, US)
- Matthew A. Warkentin (Ithaca, NY, US)
- Jesse B. Hopkins (Ithaca, NY, US)
- Andrea M. Katz (Ithaca, NY, US)
- Lois Pollack (Ithaca, NY, US)
- Robert E. Thorne (Ithaca, NY, US)
Cpc classification
International classification
G01N23/00
PHYSICS
Abstract
Apparatus and methods for performing small angle X-ray scattering (SAXS) at low (cryogenic) temperatures for determining the structure of and changes in the structure of proteins, DNA, RNA, and other biological molecules and biomolecular assemblies and structures. A cryogenic, small angle X-ray scattering (SAXS) application sample holder, includes a sample cell including a base portion and at least two parallel walls disposed on the base, wherein the sample cell has a liquid volume capacity defined by the walls and the base portion of 0.001 to 10 microliters. A method for performing cryogenic SAXS on a sample includes the steps of providing a sample biomolecule solution containing an aqueous buffer, a biomolecule, and a cryoprotectant agent, wherein the cryoprotectant agent comprises up to 60% (w/w) of the biomolecule solution, and other known components as necessary to solubilize and stabilize the biomolecule, in a sample holder of claim 1, cryogenically cooling the sample solution in the sample holder at a rate equal to or greater than 100 K/sec without ice formation, and examining the cooled sample using small angle X-ray scattering by passing a beam of X-rays through the sample.
Claims
1. A cryogenic, small angle X-ray scattering (SAXS) application sample holder, comprising: a sample cell including a base portion and at least two parallel walls disposed on the base, wherein the sample cell has a liquid volume capacity defined by the walls and the base portion of 0.001 to 10 microliters, and wherein each of the base portion and the at least two parallel walls are structured and configured to directly contact a liquid sample when in use.
2. The sample holder of claim 1, wherein the at least two parallel walls consist of two walls that are offset.
3. The sample holder of claim 1, further comprising a sample cell support structure to which the sample cell can be coupled, whereby the support structure is adapted to hold and position the sample cell in an X-ray beam, wherein the sample cell and coupled support structure have known physical characteristics allowing the sample to be cooled at a rate of at least 100 K/s.
4. The sample holder of claim 1, where the base portion and the walls are one or more of a polymer, silicon, silicon nitride, silicon dioxide, graphene.
5. The sample cell of claim 1 or 3, wherein the walls have a thickness of between 0.001 and 100 microns.
6. The sample cell of claim 5, where the at least two parallel walls have a thickness of between 0.1 and 50 microns.
7. The sample cell of claim 1 or 3, wherein the at least two parallel walls have a separation distance of between 0.1 mm and 5 mm.
8. The sample cell of claim 7, wherein the at least two parallel walls have a separation distance of between 0.5 mm and 2 mm.
9. The sample cell of claim 1 or 3, wherein the at least two parallel walls have a height and a width of between 10 and 1000 microns.
10. The sample cell of claim 9, wherein the at least two parallel walls have a height and a width of between 100 and 500 microns.
11. The sample cell of claim 1 or 3, wherein the at least two parallel walls have a maximum surface roughness of less than 10 nm.
12. The sample cell of claim 1 or 3, further comprising a contact line pinning ridge disposed along a vertical end of each of the at least two parallel walls.
13. The sample cell of claim 1 or 3, further comprising one or more side walls connected in a transverse orientation to a respective one of the at least two parallel walls.
14. The sample cell of claim 13, wherein the one or more side walls extend beyond the ends of the at least two parallel walls.
15. The sample cell of claim 13, wherein the one or more side walls are connected to the respective ends of two of the at least two parallel walls, further wherein the one or more side walls have an aperture, whereby a liquid can be injected there through and which allow a sample to expand or contract as it is cooled to cryogenic temperature and then warmed to a higher temperature.
16. The sample cell of claim 1 or 3, further comprising a support attached to an exterior face of at least one of the at least two parallel walls, whereby to support the walls and prevent their deflection or damage due to forces exerted by a sample between the walls as the sample is cooled to cryogenic temperatures and warmed.
17. The sample cell of claim 1 or 3, further comprising a plurality of sample cells having a common base portion.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
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DETAILED DESCRIPTION OF NON-LIMITING, EXEMPLARY EMBODIMENTS
(15) Embodiments of the invention include a system for cryo-SAXS measurements; methods for preparing biomolecular samples for cryo-SAXS measurements, measuring cryo-SAXS data, and analyzing this data to determine information about biomolecular structure; and sample cells and holders designed for rapid cooling of biomolecular solutions to ice-free states and for cryo-SAXS measurements.
(16) Systems for Cryo-SAXS Measurements
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(18) Slits or apertures 3 and 4 are used to define the size and shape of the beam that reaches the sample (to match the sample and sample cell dimensions), and to block X-rays scattered by interactions with gas and system components upstream of each slit or aperture, that otherwise may contribute to X-rays measured at the area detector 13. The first beam defining slit/aperture set 3 defines the X-ray beam size and shape that is transmitted to the sample. The second guard slit/aperture set 4 is set to a size slightly larger than the beam transmitted through slit set 3, and blocks X-rays scattered upstream of slit/aperture 3 that traveled obliquely through that aperture, as well as X-rays scattered by slit/aperture 3 itself. A typical beam size at the sample may be from 2 mm to as little as 10 m, depending upon the sample cell size and the X-ray optics.
(19) In conventional SAXS measurements, the sample cell is in vacuum, to reduce X-ray scattering by gas outside the sample cell. For cryo-SAXS, samples could be mounted on the end of a cryogenically cooled stage that is in vacuum, but this would complicate sample changing and make high throughput cryo-SAXS measurements challenging.
(20) In the exemplary system, the sample's temperature is maintained at cryogenic temperatures during SAXS data collection by placing the sample within a cryogenic temperature stream of flowing gas 5, which may be generated by a gas stream cryocooler 6. The gas may be nitrogen or, for lower scattering of X-rays, helium. Suitable cryocoolers manufactured by, e.g., Oxford Cryosystems, are used in cryocrystallography. Suitable cryogenic temperatures are below 180 K (to prevent ice formation on normal SAXS measurement timescales), and, advantageously, below 140 K (roughly the temperature at which pure water forms a glass and remains glassy without ice crystals forming for an indefinite time). Standard nitrogen gas stream cryocoolers typically operate at 100 K.
(21) The sample end of the upstream flight tube is terminated by a gas impermeable window 7. This window should be thinmuch thinner than the X-ray attenuation length in the material from which the window is madeto minimize X-ray beam attenuation and scattering by the window. It should also have surfaces that are free of surface roughness on length scales of 1 to 100 nm, corresponding to the range of q values of interest in SAXS measurements, so that the windows do not produce appreciable small-angle X-ray scatter. For example, the windows may be made of Si.sub.3N.sub.4 with a thickness of 200 nm.
(22) The second slit/aperture set 4 may be located within the upstream flight tube 2. It may also be located between the upstream flight tube window and the sample, including on the sample cell itself, to minimize the amount of parasitic small angle scatter transmitted through it from sources upstream of it.
(23) The X-ray beam emerging from the upstream flight tube then passes through a sample cell 8 containing the sample. The sample cell 8 may be attached via a supporting frame 9 that is connected to a rod or post 10. The sample cell, supporting frame and rod are attached to a sample stage (not shown) that allows positioning and possibly also orientation of the sample in the X-ray beam. To simplify attachment to the sample stage, the supporting frame and rod are preferably compatible with magnetic goniometer bases used in high throughput cryocrystallography, and with sample stage heads that accept magnetic goniometer bases.
(24) The direct X-ray beam and X-rays scattered by the sample and holder then proceed through a downstream window 11 (similar to the upstream window 7) and an evacuated downstream flight tube 12 (to minimize scattering of the direct beam) to an area detector 13 (e.g., a CCD detector or a pixel array detector, such as those manufactured by Rayonix and Dectris). The area detector is typically located from one to several meters from the sample, so that its area captures only X-rays scattered through small angles.
(25) A beam stop 14 of size at least slightly larger than the direct X-ray beam diameter absorbs and attenuates the direct beam so that the beam intensity at the detector does not saturate it, while allowing X-rays scattered by the sample through small angles to reach the detector.
(26) The direct X-ray beam intensity may be monitored using two detectors, one located upstream of the sample 15, and one located downstream of the sample, with the downstream direct beam measurement configured so as not to obstruct scattered X-rays. The ratio of the beam intensities at the positions of these two detectors gives the attenuation of the X-ray beam by the sample, sample cell, and other components along the beam's path. It thus depends on the X-ray beam's path length through the sample, and for samples of the same composition, can be used to determine this path length. These two detectors may be cross calibrated using a reference sample, and will advantageously have very low drift to allow accurate (better than 0.1%) relative intensity measurements.
(27) Suitable upstream detectors include a helium gas ionization chamber detector. Suitable apparatus for measuring the downstream beam intensity include a PIN diode detector directly incorporated into the beam stop 16. A calibrated beamstop that attenuates but does not fully block the direct beam can be used, so that area detector 13 can be used to measure the direct beam intensity. The beam stop may be designed to reflect some fraction of the direct beam into an off-axis detector (e.g., an X-Flash detector, a photomultiplier tube) that points toward the beam stop surface.
(28) Methods for Cryo-SAXS Measurements
(29) The methods required for cryo-SAXS on solutions of biomolecules cooled to cryogenic temperatures differ from those used in conventional, near room-temperature measurements in several important ways. The samples must be cooled to cryogenic temperatures and held at cryogenic temperatures until measurements are complete. They must be cooled in such a way that no ice or other inhomogeneities on 1 to 100 nm length scales probed in SAXS form during or after cooling are formed. This is considerably more challenging than, e.g., cooling protein crystals to cryogenic temperatures for cryocrystallographic measurements because SAXS data is far more sensitive to the presence of even minute amounts of ice than are crystallographic data.
(30) Minimizing ice formation requires that cryoprotectants such as ethanol, glycerol, ethylene glycol, polyethylene glycol, polypropylene glycol, sucrose or trehalose be added to the solutions. At high concentrations, cryoprotectants can change the structure of biomolecules. Cryoprotectant concentrations should thus be limited to below 60% w/w, and, more advantageously below 40% w/w. Our experiments show that achieving ice-free samples at such concentrations requires sample cooling rates comparable to or in excess of 100 K/s. Achieving these cooling rates requires the use of small samplesgenerally, volumes less than 10 microliters and typically less than 1 microliter; the use of sample cells with low thermal mass and good thermal conductivity; and the use of cooling methods that maximize the rate of heat transfer throughout the cooling process. The samples in general will contract or expand on cooling, and so sample cells must be designed to withstand the forces exerted upon them due to this expansion and contraction.
(31) On the other hand, radiation damage to the sample by the illuminating X-rays is dramatically reduced, so that much smaller sample volumes100 to 1000 times smaller than in conventional room-temperature SAXS measurementscan be used. The flow cells used in room-temperature SAXS are then replaced with much smaller volume, non-flow cells that can be rapidly cooled to cryogenic temperatures.
(32) The biomolecules and biomolecular structures of interest in SAXS and cryo-SAXS include proteins, nucleic acids (DNA and RNA), protein complexes, protein-nucleic acid complexes, and larger structures such as virus capsids.
(33) As in conventional SAXS measurements, in cryo-SAXS a monodisperse, aggregate-free solution of the biomolecule or complex of interest is prepared. Generally, this requires preparing a suitable buffer solution containing salts and other constituents to solubilize and stabilize the protein, and to prevent it from aggregating or precipitating. Maximum biomolecule concentrations are generally limited to below 10 mg/ml (depending upon the second virial coefficient describing intermolecular interactions in solution), to minimize effects on SAXS signals due to interparticle interference.
(34) Unlike in conventional SAXS, in cryo-SAXS cryoprotectants are added to this buffer solution to reduce the cooling rates required to prevent ice formation and to achieve a homogeneous, ice free state at cryogenic temperatures. Cryoprotectant free protein containing buffer solutions require impractically large cooling rates of approximately 10.sup.6 K/s to prevent ice formation. With cryoprotectant concentrations of 50% w/w, the required cooling rates are reduced to roughly 10-50 K/s. However, large cryoprotectant concentrations (especially above 50% w/w) may alter the biomolecule's structure. Concentrations in the range of 40% w/w and lower are thus advantageous, which may require cooling rates of at least several hundred K/s.
(35) Cryoprotectants also modify the average electron density difference or contrast between the biomolecules and the surrounding solution. This contrast determines the strength of the SAXS signal from the biomolecule, and proper choice of cryoprotectant or of a mixture of two or more cryoprotectants can maximize this electron density contrast. Contrast maximizing choices include the chain polymers polypropylene glycol and polyethylene glycol, which have electron densities of 332 and 386 electrons per cubic nanometer, comparable to that of water (334 electrons per cubic nanometer) and lower than those of most other common, biomolecule-friendly cryoprotective agents.
(36) After the biomolecule is dissolved in the cryoprotectant-containing buffer, the solution is usually centrifuged or filtered using standard methods (e.g., using size-exclusion chromatography) to eliminate aggregates.
(37) In cryo-SAXS, this solution is placed into a sample cell. The sample cell is optimized both to yield the cleanest SAXS data and to allow the fastest possible cooling of the sample, as discussed below. The sample cooling rate is ultimately limited by the sample volume and by its geometry (e.g., its surface area to volume ratio). Typical solution volumes in cryo-SAXS will be less than 10 microliters and advantageously less than 1 microliter in order to cool fast enough that cryoprotectant concentrations below 50% w/w will yield ice-free state at cryogenic temperatures. Experiments described later indicate that volumes as small as 10 nanoliters are sufficient to determine a molecular structure. The smallest feasible volume for a molecular structure determination using a single sample is determined by the amount of SAXS data that can be obtained before radiation damage to the sample becomes significant, compared with the amount of SAXS data required to determine a molecular structure. In principle, volumes as small as 1 pl (10.sup.12 liters) may be used with a synchrotron X-ray beam that is focused or defined using apertures to, e.g., a 5 m diameter, and using a cell thickness along the X-ray beam that is much smaller than the X-ray absorption length in the sample. For example, a cell with interior dimensions of 10 m10 m10 m has a volume of 1 pl. However, data from measurements on multiple samples would then need to be combined to obtain enough data to determine the molecular structure, and errors introduced by sample irreproducibility might then make accurate molecular structure determination difficult.
(38) Once the sample has been dispensed into the sample cell (e.g., using a pipette, a syringe, an automated liquid handler, an acoustic drop generator), the sample and cell are cooled to cryogenic temperature at a rate of at 100 K/s or larger. Reference is made to PCT/US2007/007963, 20090133410, System and method for increased cooling rates in rapid cooling of small biological samples, the subject matter of which is fully incorporated by reference. The cell is loaded and cooled as soon after sample filtration as is feasible (e.g., in one minute or less) to limit aggregation and also any deposition of biomolecule on the cell walls that may reduce biomolecule concentration.
(39) The sample may be cooled by inserting it into a cryogenic temperature stream of nitrogen or helium gas, including in the gas stream that is used to maintain the sample at cryogenic temperature during SAXS data collection. It may be cooled by plunging it into a cryogenic liquid such as liquid nitrogen, liquid propane, or liquid ethane, preferably held at temperatures just above their melting temperature. It may be cooled by slamming it onto a cryogenic temperature surface of a high thermal conductivity material such as copper. One advantageous method is to cool the sample and cell by plunging them into a liquid cryogen (nitrogen, propane or ethane) at a speed of roughly 1 m/s, where the cold gas layer that forms immediately above the cold liquid is removed prior to the plunge by blowing it away. This method gives the fastest possible cooling rates, approaching 10,000 K/s for 1 nl samples, as discussed in US Application 20090133410, the subject matter of which is fully incorporated herein by reference. These large cooling rates allow sample cryoprotectant concentrations needed to prevent ice formation to be minimized.
(40) After cooling to cryogenic temperatures, aggregation and sample degradation are impossible as long as the sample is maintained at temperatures below its glass transition temperature (roughly 140 K for pure water, and higher for water-cryoprotectant mixtures). In practice, temperatures below 150 K are sufficient, and so samples can be stored indefinitely in liquid nitrogen. This long sample storage time is a large advantage of cryo-SAXS over conventional SAXS on liquid samples. Many samples can be frozen and stored over an extended time period, and then measured as a group at, e.g., a synchrotron source, without concern for sample aggregation or degradation.
(41) For SAXS measurements, the sample is transferred from its storage container (or from the cryogenic temperature liquid or gas) to a cryogenic temperature stream of nitrogen or helium gas (5 in
(42) The sample is then illuminated with an X-ray beam 1, and the scattered X-rays measured using the area detector 13. At the same time, the direct beam intensity can be measured to high accuracy before and after the sample using detectors 15 and 16.
(43) A second, reference sample is prepared, dispensed into a sample cell, rapidly cooled to cryogenic temperature, and then examined using cryo-SAXS. The reference sample does not contain the biomolecule of interest, but is otherwise identical in composition to the first sample. This sample may be dispensed into the same cell as the first, biomolecule containing sample, or it may be dispensed into a different cell.
(44) Cryo-SAXS data from both the biomolecule containing and reference samples are examined to verify that both samples are ice free and free of other inhomogeneities induced by cooling. This can be assessed by examining the scattered intensity at small q, especially for the reference sample. As discussed below and as shown in
(45) If the sample cells are designed so that the path length of the X-ray beam through each sample is accurately and reproducibly fixed, then the SAXS data on the biomolecule containing and reference samples can be analyzed using methods standard in (room temperature) SAXS to determine the scattered intensity versus wave vector due to the biomolecule itself, and then to determine information about the biomolecule's structure.
(46) If the sample cell dimensions do not control or determine the path length of the X-ray beam through each sample (i.e., if the dimension of each sample along the X-ray beam path is not fixed), then this path length must be deduced for both the reference sample and biomolecule containing sample, or the path length ratio must be deduced. This ratio allows the measured scattered X-ray intensities versus wave vector q to be properly normalized, and for the normalized intensities from the biomolecule-containing and reference samples to be analyzed to determine the scattered intensity versus wave vector q for the biomolecule itself. This normalization can be performed by measuring the direct beam intensity before and after the sample, for each sample. The ratio of these intensities determines the attenuation factor for each sample, which is determined by the X-ray path length through each sample.
(47) Errors in this normalization procedure result if the sample boundaries where the beam enters and exits it are not flat and perpendicular to the beam over the area of the beam's cross-section. Thus, methods for flattening the sample boundary are useful. When the sample boundary is a meniscus formed due to contact with cell walls, the boundary can be flattened by pinning its contact lines using ridges on the interior surface of the sample cell, as discussed below, and by dispensing an accurately determined volume into the cell, just sufficient to produce a flat meniscus at the position of the contact line pinning ridges. The sample boundary can also be flattened by spinning the sample about its center of mass before and during cooling at a sufficient speed that the internal pressure generated by the spinning greatly exceeds the capillary pressure associated with surface tension responsible for curvature of its surface.
(48) Sample Holders for Cryo-SAXS Measurements
(49) The cells used to hold the biomolecule-containing and reference samples during cooling to cryogenic temperature and during cryogenic temperature SAXS data collection are a critical element in successful cryo-SAXS measurements.
(50) Conventional room-temperature SAXS measurements require large sample volumes to obtain sufficient data to determine a biomolecular structure. At room temperature, samples are damaged by relatively small X-ray doses (where dose is X-ray energy deposited per kg of sample). This sets a minimum sample volume required to determine a molecular structure that is typically at least 10 microliters and often much more. SAXS systems typically use a continuous flow cell that is fixed in place in the SAXS/X-ray apparatus. Each sample to be examined is flowed through the cell while X-rays illuminate a part of the cell. As sample flows through the X-ray beam, it becomes damaged, and the flow carries the damaged sample out of the beam. After each sample solution is measured, the cell interior is washed with an appropriate solution. The flow cells are often held in vacuum, so that removing them for cleaning and maintenance requires some effort. The cells must have two X-ray transparent windows bounding the liquid sample, whose size must be comparable to or larger than the X-ray beam used to examine the sample.
(51) Generally, biomolecule-containing solutions are transported to the SAXS setup (a small laboratory system, or a system at an X-ray beamline at a synchrotron X-ray source such as the Advanced Photon Source at Argonne National Laboratory) and then injected into the setup's fixed sample cell. Sample solutions, not sample holders loaded with sample solutions, are thus transported to the X-ray source/SAXS setup for measurements, and few sample cells are required.
(52) In both conventional SAXS and cryo-SAXS, the dimension of the sample cell and sample along the X-ray beam is typically chosen to most efficiently use the available X-ray photons in the X-ray beam to generate SAXS signal from the sample. Generally, the sample cell dimension is chosen to be comparable to the X-ray attenuation length in the sample solution at the chosen X-ray energy. This length increases from approximately 1 mm at 8 keV to 2 mm at 10 keV.
(53) In cryo-SAXS, to achieve the sample cooling rates of 100 K/s or greater needed to prevent ice formation (while using cryoprotectant concentrations that are not so large as to change the biomolecule's structure), the sample cells must have small volumespreferably less than 1 microliterand must be designed to minimize the cell's heat capacity and maximize the rate of heat transfer from the cell during cooling in a cryogenic temperature liquid or gas stream. Preferably, the cell's heat capacity should be small (less than 1 mJ/K) compared with the roughly 4 mJ/K heat capacity of 1 microliter of water (although a cell design with a large aspect ratio and large surface area to volume ratio could have a larger cell heat capacity). Our experiments show that cooling to cryogenic temperatures greatly reduces radiation damage to the biomoleculesby a factor of 100 or more. Consequently, cryo-SAXS allows the same amount of SAXS data to be obtained before radiation damage becomes excessive using a much smaller total sample volume.
(54) To achieve the fastest cooling and to minimize cryoprotectant concentrations, the sample-filled cells are preferably cooled by immersion in a liquid cryogen. The frames or other hardware used to hold the cells during cooling should not appreciably affect these cooling rates. These cells are then transported to the X-ray source/SAXS setup for measurement. Although individual cells could be thawed, cleaned and reloaded with a new sample after measurement of each sample, this would be tedious, and the time spent thawing and cleaning would be long compared with typical SAXS measurement times per sample (1 second or less at a third generation synchrotron source), and so would be a bottleneck in data collection. Instead, a large number of samples cells are loaded with a large number of reference and biomolecule-containing samples. These samples are then cooled, stored, and transported as a group for rapid sequential measurement on a cryo-SAXS setup.
(55) Consequently, cryo-SAXS sample cells should preferably be easily mass produced at modest cost, should be easy to fill using pipettes and other standard liquid handling hardware, should be easy to cool, and should be easy to transfer from a cryogenic storage container to the sample positioning stage of a SAXS setup. Preferably they are also easily cleaned (e.g., in a detergent solution, in an ultrasonic bath) and/or are of sufficiently low cost that they can be disposed after each use. A substantial infrastructure has been developed to handle cryocooled crystals mounted in nylon loops for X-ray cryocrystallography, and this infrastructure allows high throughput measurements. Sample holders for cryo-SAXS may be designed to be compatible with at least some hardware components of this infrastructure, including the sample holding and positioning stages and the magnetic goniometer bases conventionally used to quickly and easily attach samples to these stages.
(56) In the present invention, we distinguish two different kinds of sample cells for cryo-SAXS: (1) cells in which the solution is bounded in at least one direction by X-ray transparent walls or windows, and in which the X-ray beam is directed through these windows; and (2) cells in which there are no windows, walls or other obstructions along the X-ray beam path, so that the X-ray beam passes only through the sample itself.
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(58) The separation between the walls is set to be comparable to the X-ray attenuation length in the sample, to give adequate SAXS signal per incident direct beam photon and to minimize data collection times. This separation will typically be 0.5 to 2 mm and, particularly, about 1 mm, and a maximum range (depending upon X-ray energy and sample absorption) between 0.1 and 5 mm.
(59) These walls are advantageously highly X-ray transparent, which means that their thickness should be comparable to or smaller than the X-ray attenuation length in the wall material. X-ray attenuation lengths at 8 keV are roughly 70, 120, and 70 m for silicon, silicon dioxide and silicon nitride, respectively. If the walls are too thin, they are easily deflected and/or broken, including by forces exerted during processing required to fabricate the cells, by forces exerted during cleaning of the cells (e.g., by sonication), and by forces exerted by the sample between the walls as the sample contracts or expands during cooling and thawing. For silicon walls, our experiments suggest that walls larger than 15 m thick reliably survive the processes involved in cell fabrication and cleaning. Large wall deflectionsi.e., more than a fraction of 1% of the wall spacing introduce errors when subtracting SAXS signals from biomolecule containing and reference solutions. For silicon walls with heights of a few hundred micrometers, a wall thickness of 10 to 50 m provides a good compromise between X-ray transparency and rigidity.
(60) The walls should also produce very little X-ray scattering at small angles, so as to minimize background SAXS signals compared with those from the sample itself. Any inhomogeneity or nonuniformity in the walls that varies with position along or perpendicular to the wall surfaces on the length scales probed by SAXSfrom roughly 1 to 100 nmwill generate SAXS signals. Consequently, the walls should be as flat and smooth as is possible.
(61) The dimensions of the wall perpendicular to the X-ray beam directioni.e., the wall height and widthare set by the desired sample volume. For successful ice-free cooling with the smallest cryoprotectant concentrations, this volume, and thus the lateral wall dimensions, should be minimized. As discussed above, it should generally be kept below 1 microliter to give cooling rates of at least 100 K/s and to avoid cryoprotectant concentrations in excess of 50% w/w. For a 1 microliter volume, these dimensions should be less than roughly 1 mm.
(62) In many cases it will be preferable to match the lateral dimensions of the walls to the X-ray beam dimensions, since sample volume outside the beam does not contribute to the SAXS signal. A typical X-ray beam in current practice has a full width at half maximum intensity (FWHM) of roughly 100 m, although some small amount of intensity may be present (due in part to grazing incidence scattering) to a distance from beam center roughly two or three times this size. A wall height and width of between 100 to 500 m is advantageous. Total volumes defined by the walls will then be between roughly 0.01 and 1 microliter for a 1 mm cell dimension along the X-ray beam. Smaller volumesdown to 0.001 microliter and possibly smallermay be feasible when using smaller, more intense X-ray beams available at synchrotron sources, for samples with sufficiently low radiation sensitivity at cryogenic temperatures. A cell for use with a 20 m micrometer X-ray beam with cell dimensions of 0.5 mm long by 50 m by 50 m will have a volume of 1 nl. Sample cells with volumes as large as 10 microliters might yield fast enough cooling to achieve ice free and homogeneous samples for SAXS in some cases, but larger cells are not likely to be useful.
(63) Subject to the thickness and surface smoothness constraints above, the walls may be made of a wide variety of materials including those commonly used in semiconductor and microfluidic devices such as silicon, silicon nitride, silicon dioxide (quartz), graphene, and the polymers SU8, PDMS, PMMA, and polyimide. A variety of anisotropic etching processes have been developed that allow high aspect ratio (tall and thin) walls with nearly atomically smooth surfaces to be fabricated using crystalline materials including silicon. The walls could also be separately fabricated (e.g., by laser cutting thin sheets) and bonded to the base. Availability and cost of processing tools and processes are also an important consideration in materials choice.
(64) The base to which the walls are attached must be thick enough so as to be rigid, but not so thick that its thermal mass and thermal conductance appreciably slow sample cooling. Generally, a thickness of 0.1 to 1 mm should be sufficient. The base may extend beyond the footprint of the walls to facilitate sample cell handling during cooling and data collection. The base need not be X-ray transparent and need not have smooth surfaces. It can be of a variety of materials including standard materials used in semiconductor and microfluidic devices (silicon, silicon dioxide, silicon nitride, SU8 and polyimide). In particular, it can be of the same material as the walls.
(65) This basic cell design can be modified in several ways.
(66) During filling, sample liquid will tend to spread laterally along the walls and around their edges. Liquid spreading outside of the interior volume defined by the walls and base can be inhibited by adding rectangular, trapezoidal or triangular cross section ridges (19 in
(67) The surfaces of the walls can be treated, for example, by oxygen plasma etching, to make the interior wall surfaces hydrophilic, to encourage uniform sample contact with the X-ray transparent walls across the area of the X-ray beam. The exterior surfaces of the wall and base can be treated by silanizing or by applying coatings similar to the commercial product RainX to make them hydrophobic, to prevent sample spreading to these surfaces.
(68) For free-standing walls of a given height and width as in
(69) In the cell designs in
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(71) The sidewall thickness is chosen to maximize heat transfer rates from the sample while a providing adequate cell wall rigidity. They may have thicknesses from 1 to 200 m and, more particularly, in the range 25-50 m.
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(74) The sample cells described here could be fabricated using processes commonly used for semiconductor and microfluidic devices. All parts but the X-ray transparent windows could also be fabricated using stamping/embossing, micro injection molding, and possibly also 3D microprinting.
(75) In demonstration experiments, we have fabricated silicon sample cells with geometries similar to those shown in
(76) The sample cells must somehow be held during sample loading, during cooling to cryogenic temperatures, and during data collection. This may most easily and conveniently be done by attaching the cells to a frame or support. This support should be compatible with standard hardware used in crystallography for holding and positioning samples in the X-ray beam. It should also have minimal effect on sample cooling rates, which requires that it have a small thermal mass and/or have a weak thermal connection with the sample.
(77)
(78) An alternative sample cell design eliminates the X-ray transparent walls, and instead confines the sample between two walls that run parallel to rather than perpendicular to the X-ray beam. The X-ray beam then only passes through the sample and not the holder, so there is no contribution to background scatter from the holder. For example, in the cells of
(79) Initial Studies and Example Applications of Cryo-SAXS
(80) Here we describe our initial studies which led to the first successful demonstration of cryo-SAXS on biomolecules, which illustrate many of the principles and methods discussed above.
(81) Evaluation of Cryoprotectants.
(82) The cryoprotectants poly(ethylene glycol) (average molecular mass of 200 Da), glycerol, and dimethyl sulfoxide (DMSO) were purchased from Sigma (St. Louis, Mo.), and ethylene glycol was purchased from Avantor Performance Materials (Phillipsburg, N.J.). Cryoprotectant-water mixtures were prepared by weight in increments of 5%. Spherical drops of 1 microliter volume were held in a 700 m nylon loop (Hampton Research, Aliso Viejo, Calif.) and rapidly cooled by placing them in a T=100K nitrogen cryostream (700 series, Oxford Cryosystems, Oxford, United Kingdom). Cryo-SAXS data on these gas-stream-cooled samples were acquired at CHESS beamline F2. The X-ray energy was 9.88 keV, the sample-detector distance was 1.47 m and the detector was a fiber-coupled CCD (Quantum 1, Area Detector Systems Corporation, Poway, Calif.). The upstream slits and flight tube were held under vacuum and separated from the sample area by mica windows. SAXS curves were processed using BioXTAS RAW software.
(83) Preparation of Biological Samples for Cryo-SAXS.
(84) Glucose isomerase crystals (Hampton Research, Aliso Viejo, Calif.) were re-dissolved in buffer containing 100 mM Tris pH 8.0 and 1 mM MgCl2. Hen egg white lysozyme (Sigma, St. Louis, Mo.) was dissolved in buffer containing 40 mM Na-acetate pH 4.0, 50 mM NaCl and 1% (v/v) glycerol. A 24-bp DNA duplex with sequence GGTGACGAGTGAGCTACTGGGCGG (SEQ ID NO: 1) (and its complement) was made from synthetic HPLC-purified oligonucleotides (Integrated DNA Technologies, Coralville, Iowa). The complementary strands were mixed and annealed to form the duplex, following vendor instructions. The DNA was then buffer exchanged with 10 mM Na-MOPS pH 7.0 and 100 mM NaCl using a spin column (Amicon Ultra-0.5, 10,000 mol wt cutoff, EMD Millipore, Billerica, Mass.). For each matching buffer, a 2PEG solution was prepared with 946 mg/mL PEG 200. Each 2PEG solution was combined with the corresponding cryo-SAXS sample and matching buffer in a 1:1 ratio by volume, for a final concentration of 45% (w/w).
(85) Sample Holders.
(86) Two different window-free, low volume sample cells were used for cryo-SAXS. For 1 microliter volume samples, the cell (
(87) SAXS Data Collection.
(88) SAXS data from biological samples were collected using beamline G1 at CHESS, configured as shown in
(89) Because of the small sample volumes used for cryo-SAXS, background scattering originating upstream of the sample had to be minimized. The upstream flight tube was filled with He and extended to within 5 mm of the sample using 1.8 mm ID, 0.3 mm wall stainless steel tubing. To further block background scattering that could pass around the sample, the guard slits were supplemented by a 200 nm thick, 500 m square aperture Si.sub.3N.sub.4 window in a 381 m thick Si frame (Fabrication Services and Technology Ltd, Northampton, England) that was glued to the end of the tubing. Scattered X-rays were collected through an evacuated flight tube with a 200 nm thick, 2 mm square Si.sub.3N.sub.4 window with (Fabrication Services and Technology Ltd) epoxied to an aluminum cone at the upstream end. The photograph in
(90) SAXS data on biomolecules were acquired at CHESS beamline G1 during two separate runs with similar beamline configurations. In the first run, cryo-SAXS data were taken using the 1 microliter holder. A 1.52 m sample-detector distance and a 10.5 keV X-ray energy were used to probe scattering wavevectors 0.01<q<0.28 .sup.1, where q=4 sin()/, 2 is the scattering angle and is the X-ray wavelength. The beam size at the sample position was 119 m193 m (heightwidth, FWHM). The X-ray flux calculated from the current through an N.sub.2 ion chamber, placed at the beamstop position with the sample removed, was 6.310.sup.10 s.sup.1. In the second run, cryo-SAXS data from sub-microliter samples and room temperature SAXS data were acquired with an X-ray energy of 10.0 keV, a sample-detector distance of 1.35 m, a beam size of 220 m190 m (heightwidth, FWHM), and an X-ray flux of 1.010.sup.11 s.sup.1. The downstream vacuum window was Kapton film rather than Si.sub.3N.sub.4.
(91) Background Subtraction.
(92) Conventional SAXS sample cells use parallel, X-ray transparent windows to define a fixed path length. Scattering curves are collected from sample and matching buffer solutions held in identical cells, each is normalized by the transmitted X-ray intensity measured during the exposure, and the two are subtracted to obtain the macromolecule's scattering profile. The cryo-SAXS sample holders described above do not define a fixed path length for the sample, so a normalization and background subtraction method was devised to account for path length variation. The total scattering measured at the detector, I.sub.total(q), is modeled as
I.sub.total(q)I.sub.0T{log(1/T)[I.sub.M(q)+I.sub.S(q)]+I.sub.bkg(q)}
where I.sub.0 is the incident intensity, T is the X-ray transmission factor of the sample (and thus log(1/T) is proportional to the thickness), I.sub.M(q) and I.sub.S(q) are the scattering from the macromolecule and solvent, respectively, and I.sub.bkg(q) is the instrumental background scattering. With appropriate normalization, I.sub.M(q) can be found from three scattering profile measurements: one of the macromolecule-containing sample, one of the macromolecule-free buffer, and one of the empty cell. The incident and transmitted intensities, I.sub.incident and I.sub.transmitted, are measured at the same time as I.sub.total(q), and T is calculated using
T=(I.sub.incident,empty/I.sub.transmitted,empty)(I.sub.transmitted/I.sub.incident)
where the first factor, obtained from measurements of the empty cell, is included to cross-calibrate the two detectors. A background subtracted, thickness normalized intensity can be computed for both the sample (macromolecule plus buffer) and the buffer. Here background subtraction refers to removing the properly scaled instrumental background scattering:
I.sub.(q)(I.sub.total(q)/I.sub.transmittedI.sub.total,empty(q)/I.sub.transmitted,empty)(1/log(1/T)).
Then, the difference between buffer and sample curves I.sub.diff.(q)=I.sub.,sample(q)I.sub.,buffer(q) is proportional to I.sub.M(q), the scattering of the macromolecule.
Estimation of X-Ray Dose.
(93) The X-ray dose D delivered to the sample was calculated using D=t.sub.expfE(1T)/(V), where t.sub.exp is the exposure time, f is the X-ray flux (photons per second), E is the X-ray energy, T is the sample transmission factor, V is the illuminated volume, and p is the mass density. The density 1.07 g/cm.sup.3 was estimated from available data on PEG-water mixtures at room temperature. For each sample, the illuminated volume was found from VA.sup.1 log(1/T) where A is the beam area (product of width and height at FWHM) and .sup.1 is the X-ray absorption length. The absorption length was calculated from atomic absorption data and by approximating the 45% (w/w) PEG-200 water mixture as tetra(ethylene glycol) and water in a 1:13 molar ratio (i.e. H.sub.44O.sub.18C.sub.8) with the density given above; at 10 keV, .sup.12.15 mm. In X-ray crystallography, dose calculations are typically based on the mass-energy absorption coefficient. At the X-ray energies used, this calculation agrees with one based on the mass-energy absorption coefficient to within 1%.
(94) An accurate measure of the beam size at the sample position is required for computing the X-ray dose. X-ray burns in a 1 mm thick glass slide were acquired with exposures of 1, 2, 8, 16, and 32 seconds, digitized using a flatbed scanner at 12,800 dpi (EPSON Perfection 1660), and analyzed in MATLAB. The images were corrected for the nonlinear response of the glass using a calibration curve generated from the multiple exposures.
(95) Analysis of SAXS Profiles.
(96) The pair-distance distribution function P(r) was calculated from the SAXS profiles using the Bayesian Indirect Fourier Transform (BIFT) method. An indirect Fourier transform program with smoothness regularization was written in MATLAB, and Bayesian estimation was used to find the maximum particle dimension D.sub.max, the Lagrange multiplier a, and the noise level p. In addition, the evidence for the hypothesis was computed (i.e., the probability of the data given the basis set, noise model, and regularizer).
(97) Ab initio reconstructions of the low-resolution particle envelope from SAXS data were performed using the ATSAS suite of programs. For each SAXS curve, 16 DAMMIF reconstructions (fast mode, no symmetry) were aligned and averaged using DAMAVER. Each average reconstruction was aligned with its corresponding atomic structure using SUPCOMB and visualized using Pymol version 1.2rl (DeLano Scientific LLC).
(98) PEG-200 Solutions Yield Good Contrast, Low Background Scattering and Complete Vitrification of 1 L Drops.
(99) Excessive and irreproducible ice formation on cooling has been a major obstacle to cryo-SAXS. In biomolecular cryocrystallography, the cooling rate and the choice and concentration of cryoprotectant are key variables in obtaining a homogeneous, fully vitrified state at T=100K. Cryo-SAXS imposes additional constraints. Most cryoprotectants have higher electron densities than water. Adding cryoprotectant thereby increases the solvent's average electron density and its electron density fluctuations, decreasing SAXS contrast and increasing solvent background scattering. Consequently, cryoprotectants that are effective at low concentrations and that have electron densities near that of water are preferred. Based upon these criteria, we find PEG-200 to be the superior choice among several other small-molecule cryoprotectants tested (including glycerol, ethylene glycol, and DMSO).
(100) The cryoprotectant concentration necessary for complete vitrification was determined by acquiring cryo-SAXS profiles (
(101) In the SAXS data (
(102) The SAXS curves in
(103) At PEG concentrations above 45% (w/w), no ice signal was observed, and samples could be reliably vitrified. Similar measurements yielded minimum concentrations for ice-free cryo-SAXS profiles of 50% (w/w) for glycerol, 50% (w/w) for ethylene glycol, and 45% (w/w) for DMSO. These cryoprotectant concentrations are roughly 5% larger than are required to eliminate ice rings in crystallographic diffraction at comparable cooling rates.
(104) Sample Cell Design and Buffer Subtraction Technique Enable Collection of Cryo-SAXS Data from Biomolecules.
(105) The window-free, thin-wall sample cell in
(106) In conventional SAXS on liquid samples, the fixed sample cell windows precisely define the X-ray path length, allowing buffer subtraction of data acquired in the same cell. In our window-free cell, the path length depends on the sample's volume and the shape of its meniscus. A three-curve background subtraction method was used to correct for inevitable path length variations, as described above. To demonstrate this method,
(107) Radius of Gyration, Maximum Dimension, and Particle Envelope Determined by Cryo-SAXS for Glucose Isomerase.
(108) Macromolecular SAXS data are most often used to find shape information, including the radius of gyration (R.sub.g), the maximum particle dimension, and the low resolution envelope. To assess whether cryo-SAXS data are of sufficient quality for these purposes, the cryo-SAXS profile for 2 mg/ml GI was analyzed using standard techniques. The Guinier plot in
(109) To determine whether the cryoprotectant or the cooling process significantly alters the SAXS profile, data were collected at room temperature (RT) from GI in buffer with and without 45% (w/w) PEG-200 cryoprotectant. The samples were oscillated through the beam within a stationary quartz capillary to reduce radiation damage. As shown in
(110)
(111) Small differences between the 100 K and room temperature SAXS curves are observed in the Guinier region. The calculated R.sub.g is 2% larger at 100 K. This difference is small compared with experimental uncertainties, and may arise from differences in the sample geometry for 100 K and room temperature measurements, and also from residual interparticle interference at the working concentration of 2 mg/ml. However, since a protein's SAXS profile includes scattering from a hydration layer of ordered water molecules, the 2% increase in R.sub.g at 100K may reflect increased hydration water ordering, as is seen in protein crystals.
(112) SAXS from Cryocooled Glucose Isomerase is Insensitive to Large X-Ray Doses.
(113) X-ray induced changes to the macromolecule's structure or solution state must be minimized to obtain reliable SAXS profiles. In room temperature solution SAXS, a series of profiles are acquired and inspected for dose and time-dependent changes using the radius of gyration as a means of quantifying damage and determining the maximum tolerable X-ray exposure. Collecting data on vitrified samples at T100 K should eliminate radiation induced aggregation (which otherwise dominates low-angle scattering) and reduce unfolding and fragmentation.
(114) To verify that data collection at T=100K reduces the rate at which radiation damage is manifested in SAXS profiles, a series of 60 s exposures were acquired from a 1 microliter GI+buffer sample and then from a buffer-only sample. The accumulated dose for each exposure was calculated from the incident X-ray beam intensity and illuminated volume as described above. GI's SAXS profile at a given dose was obtained by subtracting a buffer curve at roughly the same dose.
(115) In cryoelectron microscopy and diffraction, in X-ray cryocrystallography, and in X-ray diffractive imaging of biological samples, all proteins show similar radiation sensitivitymeasured on a damage per dose basisat T=100K, and this should also be true in cryo-SAXS. For these other diffraction techniques, the maximum tolerable dose at T=100K to achieve a data set of a given resolution (in A) is roughly 10 MGy/A. For a SAXS data set to q=0.3 .sup.1, corresponding to a resolution of 20 , this yields a maximum tolerable dose of 200 MGy. However, analysis of SAXS data is sensitive both to loss of information at high-q and to radiation-induced changes at low-q. At room temperature, radiation damage first manifests at low-q, presumably because of molecular aggregation, fragmentation and unfolding, processes that should be strongly suppressed at T=100K. However, at large doses, microscopic inhomogeneities due to, for example, radiolytic cleavage of hydrogen and subsequent recombination and diffusion, may develop. In cryoelectron microscopy, hydrogen bubbles become evident beyond doses of 1000-10,000 electrons/nm.sup.2 corresponding to doses of 45 MGy. Recent SAXS measurements on cryo-cooled insulin crystals observed a strong increase in scatter attributed to hydrogen bubble formation beyond 70 MGy, increasing to >180 MGy for T30 K. Consequently, for typically radiation sensitive biomolecules with room temperature dose limits of 1-10 kGy, cryo-SAXS should yield dose limit increases of between two and five orders of magnitude, and corresponding reductions in minimum sample volumes.
(116) High Dose Tolerance Enables Macromolecular Envelope Reconstruction from Nanoliter Sample Volumes.
(117) Because of the greatly increased dose tolerance at T=100K, cryo-SAXS should enable dramatic reductions in minimum sample volumes for macromolecular envelope reconstruction. To demonstrate this, cryo-SAXS data were collected from non-spherical drops with thicknesses between 300 and 500 m, held within polyimide crystallography loops with a 600 m diameter a shown in
(118) With this non-ideal sample geometry, accurate buffer subtraction at high-q was more difficult to achieve than with the sample holders of
(119) To correct for these background subtraction errors in sub-microliter drops, constant offsets were chosen to maximize the so-called Bayesian evidence of the P(r) function obtained using the Bayesian Indirect Fourier Transform (BIFT) method. The implementation of BIFT is described above, and we have demonstrated its ability to correct offset errors under experimental conditions is demonstrated with synthetic data. In general it is not good practice to add offsets to SAXS profiles, as the high-q scattering is used to determine the foldedness (Kratky plot) and volume (Porod invariant) of the macromolecule. However, this correction can be done without biasing envelope reconstructions. For example, the program DAMMIN/F of the ATSAS suite by default adjusts the constant offset to account for the bead model's inability to accurately represent internal density fluctuations.
(120)
(121) The results presented here demonstrate the basic feasibility of cryo-SAXS as a method for determining structural information from macromolecules in sub-microliter volume samples. Macromolecular solutions can be cooled into a vitrified state exhibiting no excess low-q scatter, indicating the absence of ice or other inhomogeneities on the length scales probed by SAXS. Required cryoprotectant concentrations are tolerable, produce modest reductions in SAXS contrast, and (at least for the macromolecules studied here) do not affect macromolecule structure. Because aggregation, unfolding, fragmentation and other degradation processes that generate sample inhomogeneities are largely eliminated in vitrified samples, radiation damage per unit dose is reduced by at least two and as much as five orders of magnitude relative to room-temperature SAXS; unless radiation-induced hydrogen bubble formation becomes important, cryo-SAXS dose limits should be 100 MGy, substantially larger than in cryocrystallography because of the lower resolution provided by SAXS. This large decrease in radiation sensitivity allows minimum sample volumes to be reduced by a comparable factor. Solvent and instrumental background subtraction is possible even with non-ideal and non-identical macromolecule and buffer sample geometries, allowing determination of radii of gyration and structural envelopes that match results from crystallography.
(122) While elimination of ice crystallites allowed us to subtract cryo-SAXS buffer scattering at low-q, reliable buffer subtraction at high-q is still a challenge. Sample geometry nonidealities and irreproducibilities and drift in instrumental background introduce errors. However, by configuring the SAXS beamline to minimize background, designing a sample cell to optimize signal to noise, and measuring and correcting for path length variations, we were able to demonstrate reasonable buffer subtraction for 2 mg/ml GI.
(123) The primary effect of the 45% (w/w) PEG-200 concentration used here was a two-fold reduction in macromolecule contrast. However, cryoprotectants may also have effects on macromolecular structure and interactions. Cryoprotectants are osmolytes, and therefore modify water activity and macromolecule hydration. High osmolyte concentrations have been used to mimic cellular conditions in vitro, and therefore may help preserve biologically relevant solution structures over some concentration range. Glycerol is known to stabilize protein structure and prevent aggregation. PEG-200 decreases the melting temperature of nucleic acid secondary structures, but stabilizes tertiary structures. These and other effects of cryoprotectant will have to be taken into account when comparing cryo-SAXS results with those from other techniques. Smaller sample volumes and the use of liquid nitrogen or propane instead of gaseous nitrogen as the cooling agent should allow cryoprotectant concentrations to be decreased below 30%, comparable to values routinely used in cryocrystallography and generally assumed to have negligible effects on macromolecule structure.
(124) The use of cryocooling has the potential to eliminate many difficulties associated with room temperature SAXS experiments. Because vitrified samples are much more radiation tolerant, they may yield much larger integrated signal to noise with no concerns about damage. Samples that spontaneously aggregate or otherwise degrade with time can be frozen immediately after manufacture and stored indefinitely, eliminating concerns about long-term stability. Dramatically reduced sample volume requirements will facilitate mass screening of solution conditions for their effects on molecular structure or association, and combinatorial binding assays to, e.g., elucidate pathways for macromolecular complex formation. Short turnaround times will result from exploiting the existing infrastructure for high-throughput crystallography, including mail-in facilities that employ automated sample handling and full brightness, for rapid turn-around data collection. In the same way that cryo-MX has transformed atomic resolution studies, cryo-SAXS is poised to transform low-resolution studies of macromolecular structure and function.
(125) All references, including publications, patent applications, and patents, cited herein are hereby incorporated by reference to the same extent as if each reference were individually and specifically indicated to be incorporated by reference and were set forth in its entirety herein.
(126) The use of the terms a and an and the and similar referents in the context of describing the invention (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms comprising, having, including, and containing are to be construed as open-ended terms (i.e., meaning including, but not limited to,) unless otherwise noted. The term connected is to be construed as partly or wholly contained within, attached to, or joined together, even if there is something intervening.
(127) The recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein.
(128) All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., such as) provided herein, is intended merely to better illuminate embodiments of the invention and does not impose a limitation on the scope of the invention unless otherwise claimed.
(129) No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the invention.
(130) It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit and scope of the invention. There is no intention to limit the invention to the specific form or forms disclosed, but on the contrary, the intention is to cover all modifications, alternative constructions, and equivalents falling within the spirit and scope of the invention, as defined in the appended claims. Thus, it is intended that the present invention cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents.