Disposable Fluid Circuits for Extracorporeal Photopheresis with Integrated Source of Photoactive Agent

Abstract

Disposable fluid circuits, systems utilizing disposable fluid circuits and methods for combining a treating agent with separated blood component are disclosed. The treating agent is contained within a container and the disposable fluid circuit includes one or more frangible cannulas located in one or more flow paths of the circuit that are selectively opened to establish flow communication between the treating agent container and a treatment container, and/or a separation chamber or other container.

Claims

1. A disposable fluid circuit for combined association with a cell separator and a separate irradiation device comprising: a) a patient access device for establishing flow communication with a patient; b) a cell separation chamber in openable fluid communication with said patient access device; c) a treatment container comprising walls made of a material that is transparent to light of a selected wavelength; d) tubing defining a sterile flow path between said separation chamber and said treatment container, said flow path having length sufficient to allow for placement of said separation chamber within said separator and allow for placement of said treatment container is said separate irradiation device; e) a container of photoactive agent integral with said circuit, said container having an outlet in openable flow communication with an outlet flow path; and f) a frangible cannula for preventing flow of photoactive agent from said container of photoactive agent to said treatment container.

2. The disposable fluid circuit of claim 1 further comprising a frangible cannula for preventing flow to said container of photoactive agent.

3. The disposable fluid circuit of claim 2 wherein at least one of said frangible cannulas is disposed in at least said inlet or outlet.

4. The disposable fluid circuit of claim 1 wherein said container of photoactive agent is located between said separation chamber and said treatment container.

5. The disposable fluid circuit of claim 1 further comprising a plasma container in openable flow communication with said separation chamber and said treatment container.

6. The disposable fluid circuit of claim 5 wherein said container of photoactive agent is located between said separation chamber and said plasma container.

7. The disposable fluid circuit of claim 6 comprising a frangible cannula located in a flow path between said container of photoactive agent and said separation chamber and a frangible cannula located in a flow path between said container of photoactive agent and said treatment chamber.

8. The disposable fluid circuit of claim 6 comprising a frangible cannula located in a flow path between said plasma container of photoactive agent and a frangible cannula located in a flow path between said container of photoactive agent and said treatment container.

9. A system for performing a therapeutic cellular treatment comprising: a) a reusable separator unit for separating blood into blood components; b) a reusable irradiation unit for treating a separated blood component; c) a controller configured to effect the separation of blood into blood components, effect the conveying of the separated blood components through a disposable fluid circuit and, optionally, to effect the treatment of said separated component; d) a disposable fluid circuit for combined association with a cell separator and a separate irradiation device comprising: i. a patient access device for establishing flow communication with a patient; ii. a cell separator comprising a separation chamber in openable fluid communication with said patient access device; iii. a treatment container comprising walls made of a material that is transparent to light of a selected wavelength; iv. tubing defining a sterile flow path between said separation chamber and said treatment container said flow path having length sufficient to allow for placement of said separation chamber within said separator and allow for placement of said treatment container is said separate irradiation device; v. a container of photoactive agent integral with said circuit, said container having an outlet in openable flow communication with an outlet flow path; and vi. a frangible cannula for preventing flow of photoactive agent from said container of photoactive agent to said treatment container, e) an automated cannula breaker configured to receive said cannula.

10. The system of claim 9 wherein said disposable fluid circuit further comprises a frangible cannula for preventing flow to said container of photoactive agent.

11. The system of claim 10 wherein said automated cannula breaker is configured to receive both cannulas.

12. The system of claim 9 wherein said automated cannula breaker is carried by said reusable separator unit.

13. The system of claim 9 wherein said automated cannula breaker is carried by said reusable irradiation unit.

14. The system of claim 9 wherein said controller is configured to effect breaking of one or more cannulas when a pre-determined volume of a target blood component has been collected in said treatment container.

15. A method for combining a treating agent with a collected blood component comprising: a) mounting an integral disposable fluid circuit comprising a one or more vascular access devices, a separation chamber, a treatment container and a container of a treating agent on a separator unit and an irradiation unit wherein said separation chamber is mounted within said separator unit and said treatment container is mounted within said irradiation unit; b) introducing whole blood into said separation chamber; c) separating whole blood into blood components wherein one of said blood components comprises mononuclear cells; d) removing said mononuclear cells from said separation chamber; e) conveying said mononuclear cells from said separation chamber to said treatment container; and f) opening a flow path between said treating agent container and said treatment container.

16. The method of claim 15 comprising opening a flow path between said separation chamber and said treating agent container.

17. The method of claim 15 wherein said disposable fluid circuit further comprises a plasma collection container and said method comprises separating a plasma component within said separation chamber and conveying said separated plasma to said plasma container.

18. The method of claim 17 comprising opening a flow path between said plasma container and said treating agent container.

19. The method of claim 17 comprising pumping a selected volume of said plasma component from said plasma container to said treatment container through said treating agent container.

20. The method of claim 16 comprising opening said frangible cannulas simultaneously.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0012] FIG. 1 is a diagram of the system and method for processing a mononuclear cell product in accordance with the present disclosure;

[0013] FIG. 2 is a partial perspective view of the front panel of a multifunctional apheresis separator useful in the methods and systems described herein with a portion of the disposable fluid circuit mounted thereon;

[0014] FIG. 3 is a diagram of one embodiment of a disposable fluid circuit suitable for use with the system described herein;

[0015] FIG. 4 is a perspective view of a container with separation chamber of the fluid circuit used with a separator;

[0016] FIG. 5 is a schematic view of the control circuitry, including the controller of the system of FIG. 1;

[0017] FIGS. 6(a)-6(b) are enlarged views of a treatment container and treating agent container of the fluid circuit of FIG. 3;

[0018] FIG. 7 is a diagram of another embodiment of a disposable fluid circuit suitable for use with the system of the present disclosure;

[0019] FIG. 8 is an enlarged view of a treatment container of the fluid circuit of FIG. 7 with the integral, in-line, treating agent container in openable flow communication therewith;

[0020] FIG. 9 is a diagram of still another embodiment of a disposable fluid circuit suitable for use with the system of the present disclosure;

[0021] FIG. 10 is a perspective view of an automated cannula breaker for use with the system of the present disclosure;

[0022] FIG. 11 is a view of the automated cannula breaker in association with an in-line pouch or bag of photoactive agent;

[0023] FIG. 12 is a flow chart setting forth the method of combining a treating agent with a collected blood component using, for example, the disposable fluid circuit of FIG. 3; and

[0024] FIG. 13 is a flow chart setting forth the method of combining a treating agent with a collected blood component using, for example, the disposable fluid circuit of FIGS. 7 and 8.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0025] The present disclosure is directed to disposable fluid circuits, methods, and systems for the processing of mononuclear cells in an ECP treatment. The circuits, methods, and systems of the present disclosure are described in connection with particular apheresis and irradiation/illumination devices for purposes of exemplification only. It will be understood that the methods and systems described and claimed herein may be carried out and provided in combination with other apheresis and/or irradiation/illumination devices that will be known to those of skill in the art.

[0026] Turning now to the Figures, FIG. 1 diagrammatically shows a system and the flow of fluid in the method described herein. In accordance with the present disclosure, the system includes a reusable separation unit or separator 10 and a treatment or irradiation unit 20. In one embodiment, irradiation unit 20 is independent and housed separately from separator 10. Although separately housed and shown as independent, stand-alone devices, it is preferable that separator 10 and irradiation device 20 be located adjacent to each other. While FIG. 1 shows a preferred embodiment of the individual separation and irradiation units, it will be appreciated that the methods described herein may also be used with devices having integrated separation and irradiation components housed in one device.

[0027] As generally shown in FIG. 1, whole blood is withdrawn from the patient 100 and introduced into the separator 10 where the whole blood is separated to provide a target cell population. More particularly, whole blood is withdrawn from the patient through venipuncture needle 82 (FIG. 3) and introduced into the separation chamber of separation container 12 carried within and/or mounted on a centrifuge device of separator 10. Within the separator 10, the target cell population is separated from other components. In a preferred embodiment in accordance with the present disclosure, the target cell population is the patient's mononuclear cells (MNC). Other components separated from the whole blood in this initial separation, such as red blood cells, plasma and platelets, may be returned to the patient or collected in pre-attached containers of the blood processing set, as shown by line 11. The collection of mononuclear cells is more specifically described in U.S. Pat. No. 6,027,657, the contents of which is incorporated herein by reference.

[0028] The separated target cell population, e.g., mononuclear cells with residual red blood cells and plasma, is then prepared for treatment and irradiation in reusable treatment component or irradiation unit 20. In accordance with the present disclosure, effective treatment of the mononuclear cells with ultraviolet light requires that the collected mononuclear cells be provided in a suspension having a suitable hematocrit, i.e., a certain (low) concentration of red blood cells. Specifically, the hematocrit level in the MNC suspension to be treated affects the amount of UV light that the MNC are exposed to as the red blood cells in the MNC suspension will block at least a portion the UV light from reaching the targeted MNCs. The hematocrit level of the MNC product to be treated may be adjusted by diluting the collected MNC product with plasma and/or saline, as described in U.S. Pat. No. 9,399,093 and U.S. Patent Application Publication No. US2014/0370491, both previously incorporated by reference.

[0029] Turning now, more specifically, to one embodiment of the reusable hardware units and disposable fluid circuit components of the system, devices/separators 10 useful in the collection (and washing) of mononuclear cells include the Amicus Separator made and sold by Fresenius-Kabi USA, of Lake Zurich, Ill. Mononuclear cell collections using a device such as the Amicus are described in greater detail in U.S. Pat. No. 6,027,657, previously incorporated by reference herein in its entirety.

[0030] Briefly, FIG. 2 shows a representative blood centrifuge device/ separator 10 with fluid circuit 200 mounted thereon. The fluid circuit 200 includes a blood processing container 14 (see FIG. 3) defining a separation chamber 12 suitable for harvesting mononuclear cells from whole blood. As shown in FIG. 2, a portion of disposable processing set or fluid circuit 200 is mounted on the front panel of device/separator 10. As also shown in FIG. 3, separation chamber 12, which is integral with the rest of circuit 200, is defined by the walls of a flexible processing container 14 carried within an annular gap defined by a rotating spool element 18 and an outer bowl element (not shown) housed within the cabinet of device/separator 10. The processing container 14 takes the form of an elongated tube or belt which is wrapped about the spool element 18 before use. The bowl and spool element 18 are pivoted on a yoke between an upright position and a suspended position, also not shown. In operation, the centrifuge device within separator 10 rotates the suspended bowl and spool element 18 about an axis, creating a centrifugal field within the processing chamber of container 14. Details of the mechanism for causing relative movement of the spool 18 and bowl elements as just described are disclosed in U.S. Pat. No. 5,360,542 entitled Centrifuge with Separable Bowl and Spool Elements Providing Access to the Separation Chamber, which is also incorporated herein by reference.

[0031] With reference to FIGS. 2-3 (as well as FIGS. 7 and 9), fluid circuit 200 (200, 200) includes a plurality of processing fluid flow cassettes 23L, 23M and 23R with tubing loops for association with peristaltic pumps 24 on device 10. As described in U.S. Pat. No. 6,027,657, previously incorporated by reference, cassettes 23L, 23M and 23R include molded plastic bodies with integrally molded liquid flow channels. Valve stations (depicted as numbered circles within each cassette and best seen in FIG. 7) are molded into the backside of cassette bodies. A flexible diaphragm covers and seals the backside of the cassette (23) body. Valve stations align with valve actuators of pump stations (PSL, PSM and PSR) located on the front panel of device 10. Fluid circuit 200 also includes a network of tubing and pre-connected containers for establishing flow communication with the patient and for processing and collecting fluids and blood and blood components, as shown in greater detail in FIG. 2.

[0032] The system described herein may, optionally, further include one or more automated cannula breaker(s) 250, as shown in FIGS. 10-11 and described in U.S. Patent Application Publication No. US2014/0263529, which is incorporated herein by reference in its entirety. Cannula breaker is configured to receive frangible cannulas commonly used in blood processing fluid circuits to open otherwise closed fluid paths. In accordance with the system described herein, cannula breakers 250 may be a stand-alone unit or more preferably, may be integral with or otherwise carried by separation unit 10 or irradiation unit 20. As described below, automated cannula breakers may relieve the operator of manually breaking the frangible cannulas such as those located between the container 69 of treating agent and treatment container 68.

[0033] As further seen in FIGS. 2 and 3, in one embodiment disposable processing set 200 may include a container 60 for supplying anticoagulant, a waste container 62 for collecting waste from one or more steps in the process for collecting, treating and/or washing mononuclear cells, a container 64 for holding saline or other priming or conditioning medium, a container 66 for collecting plasma, a container 68 for collecting the mononuclear cells and, preferably, container or pouch 69 for holding the photoactivation agent. As shown in FIG. 3 and, in greater detail, in FIGS. 6(a) and 6(b), container 69 may be integrally pre-attached to treatment container 68 and in openable fluid communication therewith. A frangible cannula 71 may be included in flow path 73 between containers 68 and 69 and broken by the operator to combine the photoactive agent with the collected MNCs in container 68. Alternatively, the portion of the flow path between containers 68 and 69 may be mounted onto automated cannula breaker 250 described above and shown in FIGS. 10-11, and described in greater detail in U.S. Patent Application Publication No. US2014/0263529, previously incorporated by reference. Cannula breaker 250 may be activated to open flow path 73 by operator control or by a pre-programmed command received from the system controller (described below) at a pre-determined time. For example, once weight scale 93 holding container 68 detects that a suitable volume of MNCs has been collected, controller 300 may effect breakage of cannula 71, thereby establishing flow from container 69 to container 68.

[0034] Container 68 may also serve as the illumination container, and is preferably pre-attached to the disposable circuit 200. Alternatively, container 68 may be attached to set 200 by known sterile connection techniques, such as sterile docking or the like. With reference to FIG. 3, fluid circuit includes inlet line 72, an anticoagulant (AC) line 74 for delivering AC from container 60, an RBC line 76 for conveying red blood cells from chamber 12 of container 14 to container 67, a platelet-poor plasma (PPP) line 78 for conveying PPP to container 66 and line 80 for conveying mononuclear cells to and from separation chamber 14 and collection/illumination container 68. The fluid processing circuit includes one or more patient access device(s) such as venipuncture needle(s) for accessing the circulatory system of the patient. As shown in FIG. 3, fluid circuit 200 includes inlet needle 70 and return needle 82. In an alternative embodiment, a single needle can serve as both the inlet and outlet needle.

[0035] Container 68 is suitable for irradiation by light of a selected wavelength. By suitable for irradiation it is meant that the walls of the container are sufficiently transparent to light of the selected wavelength to activate the photoactive agent. In treatments using UVA light, for example, container walls made of ethylene vinyl acetate (EVA) are suitable. Accordingly, as indicated above, container 68 in which the mononuclear cells are collected may serve both as the collection container and the irradiation container. Container 68 may be placed inside irradiation unit 20 by the operator or, more preferably, may be placed inside the irradiation chamber of irradiation device 20 at the beginning of the ECP procedure and prior to whole blood withdrawal (as shown by the broken lines representing device 20 in FIG. 2). In any event, container 68 preferably remains integrally connected to the remainder of fluid circuit 200 during the entire procedure, thereby maintaining the closed or functionally closed condition of fluid circuit 200.

[0036] Fluid flow through fluid circuit 200 is preferably driven, controlled and adjusted by a microprocessor-based controller in cooperation with the valves, pumps, weight scales and sensors of device 10, the details of which are described in the previously mentioned U.S. Pat. No. 6,027,657. As described below, the controller is programmed to activate rotation of pumps (and control the rotational speed thereof), associated with cassettes 23L, 23M and 23R, open and close valves, receive output signals from sensors and detectors, such as the interface detection system described below, and preferably, to commence and control treatment of the MNC in irradiation unit 20.

[0037] FIG. 5 is a schematic view of the control unit or controller 300 included in device 10 of the present disclosure. The controller 300 may include a microprocessor 304 (which may include multiple physical and/or virtual processors). According to other embodiments, the controller 300 may include one or more electrical circuits designed to carry out the actions described herein. In an embodiment, controller 300 may include a microprocessor and other circuits or circuitry. In addition, the controller 300 may include one or more memories 306. The instructions by which the microprocessor 304 is programmed may be stored on the memory 306 associated with the microprocessor 304, which memory/memories 306 may include one or more tangible non-transitory computer readable memories, having computer executable instructions stored thereon, which when executed by the microprocessor 304, may cause the microprocessors 304 to carry out one or more actions as described herein.

[0038] As is also illustrated in FIG. 5, controller 300 may be coupled to one or more of the structures described herein, for example to receive information (e.g., in the form of signals) from these structures or to provide commands (e.g., in the form of signals) to these structures to control the operation of the structures. As illustrated in FIG. 5, the controller 300 may be coupled to weight scales 93 (seen in FIG. 3) that hold solution containers or that are provided to collect separated blood components, the sensors associated with device 10, or more specifically with the cassettes 24L, 24M, and 24R, the valve assemblies 132, and the at least one input 302 to receive information from those devices. Additionally, the controller 300 may be coupled to the pumps 24 and the separator (centrifuge) drive unit (not shown) to provide commands to those devices and to control their operation. As further shown in FIG. 5, controller 300 may also be coupled to automated cannula breaker 250. It may also be possible that the controller 300 receives information from and provides commands to a given structure, such as one of the structures already mentioned. The controller 300 may be directly electrically connected to these structures to be coupled to them, or the controller 300 may be directly connected to other intermediate equipment that is directly connected to these structures to be coupled to them.

[0039] The at least one input 302 may include a number of different devices according to the embodiments described herein. For example, the input 302 could include a keyboard or keypad by which a user may provide information and/or instructions to the controller 300. Alternatively, the input 302 may be a touch screen, such as may be used in conjunction with a video display 308 (FIG. 1) that is disposed on the front panel of the device 10, the video display 308 also being coupled to the controller 300. The assembly of the input/touch screen 302 and video display 308 may be one of the aforementioned structures to which the controller 300 is coupled from which the controller 300 receives information and to which the controller 300 provides commands.

[0040] The method of using the FIGS. 3, 6(a) and 6(b) embodiment of disposable fluid circuit 200 to combine the photoactive agent with the collected MNCs in container 68 is generally set forth in FIG. 13. As shown in FIG. 12, upon commencement of an ECP procedure (step 400), MNCs are collected in in treatment container 68 and additional plasma and/or saline may added, as necessary, to arrive at a treatment-ready MNC product (step 402). Cannula 71 is then broken to allow the photoactive agent within container 69 to drain into container 68 (step 404). As described above, breaking of frangible cannula 71 may be performed manually by the operator or automatically by an automated cannula breaker 250, either by an operator-entered instruction or under the command of controller 300 once the system has sensed that a predetermined amount of MNCs has been collected in treatment container 68. The amount or concentration of photoactive agent in container 69 may be pre-determined based on the volume of collected MNCs and conditioning fluid. While the volume of the treating agent is fixed, the number of collected MNCs per procedure may vary. The system may be programmed to receive targeted collection amounts of MNCs and may further be programmed to cease the collection cycle once the target has been reached. Once the treating agent has drained into treatment container 68, container 69 may be sealed off from the remainder of the circuit (Step 406) and treatment of the collected cell product may proceed (Step 408).

[0041] FIGS. 7 and 8 show a different embodiment of a disposable fluid circuit 200 that can include an integrated treating agent container 69. In FIGS. 7 and 8, container 69 is shown in-line with the tubing 80 that defines a flow path and provides flow communication between separation chamber 12 of container 14 and treatment container 68. Fluid circuit 200 includes a frangible cannula located at the outlet of container 69 or located elsewhere between treating agent container 69 and treatment container 68. Fluid circuit 200 may include a second frangible cannula 71 located at the inlet of treatment container 68 or located elsewhere between treatment container 68 and treating agent container 69.

[0042] The method of using disposable fluid circuit 200 is generally set forth in FIG. 13. Upon commencement of the ECP procedure (step 500), both cannulas 71 are broken at or just before the time that the separated MNCs from the first cycle of normal MNC collection begin exiting (pumped from) separation chamber of separation container 14. As MNCs from separation chamber 12, through cassette 24R and now-open line 80, they enter in-line treating agent container 69 where they are mixed with the photoactive agent and the mixture is collected in treating container 68 (step 504). As in the method of using disposable fluid circuit of FIGS. 3 and 6(a) and 6(b), the concentration of photoactive agent in container 69 may be pre-determined based on the volume of collected MNCs to which plasma and/or saline are or have been added to arrive at a treatment-ready MNC product having a suitable hematocrit for effective treatment.

[0043] As described in connection with the earlier embodiments, breaking of frangible cannula 71 may be manually performed by the operator or automatically by an automated cannula breaker 250, either by an operator-entered instruction or under the command of controller 300 once the system has sensed that MNCs are being removed from separation chamber 12. For example, where an automated cannula breaker is used, breakage may be prompted by the optical sensor sensing the MNCs traveling through a predetermined portion of the disposable fluid circuit flow path.

[0044] A further alternative embodiment of a disposable fluid circuit 200 with an integrated treating agent container 69 is shown in FIG. 9. As in the embodiment of FIGS. 7 and 8, treating container 69 is an in-line container integral with fluid circuit 200. However, in this embodiment, container 69 is located between plasma container 66 and cassette 23R. Plasma container collects plasma separated from whole blood during initial mononuclear cell collection. The plasma collected in container 66 may be used to adjust the volume of the collected MNCs and arrive at a suitable hematocrit. Saline from the initial priming of circuit 200 may likewise be collected in plasma container 66 and used to adjust the volume of the MNC product and arrive at a treatment-ready MNC product, as described in simultaneously filed provisional patent application entitled METHODS AND SYSTEMS FOR MAINTAINING PATIENT FLUID BALANCE DURING AN EXTRACORPOREAL THERAPEUTIC CELL TREATMENT, identified by Attorney Docket number F-6782(9360-0547), which is incorporated by reference herein in its entirety. Similar to the embodiment of FIGS. 7 and 8, treating agent container 69 includes two frangible cannulas 71 located on the either side of the container either at the inlet and outlet elsewhere between plasma container 66 and treating agent container 69 and between treating agent container 69 and cassette 23R. As described in connection with the earlier embodiments, breaking of frangible cannulas 71 may be manually performed by the operator or automatically by an automated cannula breaker 250, either by operator instruction or under the command of controller 300, once the system has sensed that platelet poor plasma is being removed from separation chamber 12 during initial whole blood processing or when saline that was used to prime circuit 200 is being diverted to plasma container 66. Thus, in this embodiment, the treating agent is combined with the diluting solution (e.g., plasma/saline) and subsequently conveyed to treatment container 68 and the amount of the treating agent delivered to the MNC can be varied by pumping more or less of the diluting solution to the MNC in treatment container 68 (whereas in the previous embodiments, the amount of treating agent delivered is fixed).

Other Aspects

[0045] There are additional aspects to the methods and systems described herein including, without limitation, the following aspects.

[0046] Aspect 1. A disposable fluid circuit for combined association with a cell separator and a separate irradiation device including: a patient access device for establishing flow communication with a patient; a cell separation chamber in openable fluid communication with said patient access device; a treatment container comprising walls made of a material that is transparent to light of a selected wavelength; tubing defining a sterile flow path between the separation chamber and the treatment container, the flow path having length sufficient to allow for placement of the separation chamber within the separator and allow for placement of the treatment container in the separate irradiation device; a container of photoactive agent integral with the circuit, the container having an outlet in openable flow communication with an outlet flow path; and a frangible cannula for preventing flow of photoactive agent from the container of photoactive agent to the treatment container.

[0047] Aspect 2. The disposable fluid circuit of Aspect 1 further including a frangible cannula for preventing flow to the container of photoactive agent.

[0048] Aspect 3. The disposable fluid circuit of Aspect 2 wherein at least one of the frangible cannulas is disposed in at least the inlet or outlet.

[0049] Aspect 4. The disposable fluid circuit of any one of Aspect 1 through 3 wherein the container of photoactive agent is located between the separation chamber and the treatment container.

[0050] Aspect 5. The disposable fluid circuit of any one of Aspect 1 through 3 further comprising a plasma container in openable flow communication with the separation chamber and the treatment container.

[0051] Aspect 6. The disposable fluid circuit of Aspect 5 wherein the container of photoactive agent is located between the separation chamber and the plasma container.

[0052] Aspect 7. The disposable fluid circuit of Aspect 6 including a frangible cannula located in a flow path between the container of photoactive agent and the separation chamber and a frangible cannula located in a flow path between the container of photoactive agent and the treatment chamber.

[0053] Aspect 8. The disposable fluid circuit of claim 6 including a frangible cannula located in a flow path between the plasma container of photoactive agent and a frangible cannula located in a flow path between the container of photoactive agent and the treatment container.

[0054] Aspect 9. A system for performing a therapeutic cellular treatment including: a reusable separator unit for separating blood into blood components; a reusable irradiation unit for treating a separated blood component; a controller configured to effect the separation of blood into blood components, effect the conveying of the separated blood components through a disposable fluid circuit and, optionally, to effect the treatment of the separated component; a disposable fluid circuit for combined association with a cell separator and a separate irradiation device comprising: a patient access device for establishing flow communication with a patient; a cell separator comprising a separation chamber in openable fluid communication with the patient access device; a treatment container comprising walls made of a material that is transparent to light of a selected wavelength; tubing defining a sterile flow path between the separation chamber and said treatment container the flow path having length sufficient to allow for placement of the separation chamber within the separator and allow for placement of the treatment container is the separate irradiation device; a container of photoactive agent integral with said circuit, the container having an outlet in openable flow communication with an outlet flow path; and a frangible cannula for preventing flow of photoactive agent from the container of photoactive agent to the treatment container, an automated cannula breaker configured to receive the cannula.

[0055] Aspect 10. The system of Aspect 9 wherein the disposable fluid circuit further includes a frangible cannula for preventing flow to the container of photoactive agent.

[0056] Aspect 11. The system of Aspect 10 wherein the automated cannula breaker is configured to receive both cannulas.

[0057] Aspect 12. The system of any one of Aspects 9 through 11 wherein the automated cannula breaker is carried by said reusable separator unit.

[0058] Aspect 13. The system of any one of Aspects 9 through 11 wherein the automated cannula breaker is carried by the reusable irradiation unit.

[0059] Aspect 14. The system of any one of Aspects 9 through 13 wherein the controller is configured to effect breaking of one or more cannulas when a pre-determined volume of a target blood component has been collected in the treatment container.

[0060] Aspect 15. A method for combining a treating agent with a collected blood component including: mounting an integral disposable fluid circuit comprising a one or more vascular access devices, a separation chamber, a treatment container and a container of a treating agent on a separator unit and an irradiation unit wherein the separation chamber is mounted within the separator unit and said treatment container is mounted within the irradiation unit; introducing whole blood into the separation chamber; separating whole blood into blood components wherein one of the blood components comprises mononuclear cells; removing said mononuclear cells from the separation chamber; conveying said mononuclear cells from the separation chamber to the treatment container; and opening a flow path between the treating agent container and the treatment container.

[0061] Aspect 16. The method of Aspect 15 comprising opening a flow path between the separation chamber and the treating agent container.

[0062] Aspect 17. The method of Aspect 15 wherein the disposable fluid circuit further comprises a plasma collection container and the method comprises separating a plasma component within the separation chamber and conveying the separated plasma to the plasma container.

[0063] Aspect 18. The method of Aspect 17 including opening a flow path between the plasma container and the treating agent container.

[0064] Aspect 19. The method of any one of Aspects 17 through 18 including pumping a selected volume of the plasma component from the plasma container to the treatment container through the treating agent container.

[0065] Aspect 20. The method of any one of Aspects 16 through 19 including opening the frangible cannulas simultaneously.

[0066] It will be understood that the embodiments and examples described above are illustrative of some of the applications or principles of the present subject matter. Numerous modifications may be made by those skilled in the art without departing from the spirit and scope of the claimed subject matter, including those combinations of features that are individually disclosed or claimed herein. For these reasons, the scope hereof is not limited to the above description but is as set forth in the following claims, and it is understood that the claims may be directed to the features thereof, including as combinations of features that are individually disclosed or claimed herein.