Wet tissue containing hot water extract of Coptidis rhizoma extracted under high temperature and high pressure conditions

Abstract

The present invention relates to a wet tissue containing a hot water extract of Coptidis Rhizoma extracted under high temperature and high pressure conditions, or a distillate thereof, and more specifically, to a wet tissue containing a hot water extract of Coptidis Rhizoma obtained by mixing 2,000-8,000 parts by weight of water on the basis of 100 parts by weight of Coptidis Rhizoma and extracting the same under high temperature and high pressure conditions of 120-131 C. and 1.2-2.8 atm, respectively, or a distillate thereof. The wet tissue containing a hot water extract of Coptidis Rhizoma or a distillate thereof shows remarkable antibacterial and fungicidal activities against pathogenic microorganisms existing in the skin and has an excellent inflammation inhibitory effect, and thus can be readily used for cleaning the skin and alleviating various inflammatory diseases.

Claims

1. A method for manufacturing a wet tissue containing a Coptidis rhizome-containing composition (CR-composition), wherein the CR-composition is a hot-water extract of Coptidis rhizome or a distillate thereof; the method comprising the steps of: (i) mixing 100 parts by weight of Coptidis rhizoma with 2000-8000 parts by weight of water and extracting under a high temperature condition of 120-131 C. and a high pressure condition of 1.2-2.8 atm to prepare the hot-water extract of Coptidis rhizome, and optionally distilling the hot-water extract of Coptidis rhizome to obtain vapor, and condensing the obtained vapor, thereby preparing the distillate of the hot-water extract of Coptidis rhizome; (ii) spraying 200-400 parts by weight of the CR-composition of Coptidis rhizoma prepared in step (i) onto 100 parts by weight of a fabric for wet tissue to absorb the CR-composition into the fabric for wet tissue; and (iii) hermetically packaging and sterilizing the fabric for wet tissue absorbed with the CR-composition of Coptidis rhizome.

2. The method of claim 1, wherein the CR-composition is the hot-water extract.

3. The method of claim 1, wherein the CR-composition is the distillate, and the method comprises the step of distilling the hot-water extract of Coptidis rhizome to obtain vapor, and condensing the obtained vapor, thereby preparing the distillate of the hot-water extract of Coptidis rhizome.

4. A method for manufacturing an article selected from the group consisting of a cotton swab, gauze, a mask, a diaper and a sanitary napkin, the method comprising the steps of: (i) mixing 100 parts by weight of Coptidis rhizoma with 2000-8000 parts by weight of water and extracting under the high temperature and high pressure conditions of 120-131 C. and 1.2-2.8 atm to prepare a hot-water extract of Coptidis rhizome; and optionally distilling the hot-water extract of Coptidis rhizome to obtain vapor, and condensing the obtained vapor, thereby preparing a distillate of hot-water extract of Coptidis rhizome; (ii) spraying 200-400 parts by weight of a CR-composition wherein the CR-composition is the hot-water extract or the distillate thereof prepared in step (i) onto 100 parts by weight of one material selected from the group consisting of cotton, fabric for gauze, cotton fabric, natural fiber fabric, synthetic fiber fabric, mixed fiber fabric and nonwoven fabric to absorb the CR-composition into the selected material; (iii) drying and sterilizing the material absorbed with the CR-composition; and (iv) manufacturing an article selected from the group consisting of a cotton swab, gauze, a mask, a diaper and a sanitary napkin using the dried and sterilized material of step (iii), and packaging and sterilizing the manufactured article.

5. The method of claim 4, wherein the CR-composition is the hot-water extract.

6. The method of claim 4, wherein the CR-composition is the distillate, and the method comprises the step of distilling the hot-water extract of Coptidis rhizome to obtain vapor, and condensing the obtained vapor, thereby preparing the distillate of the hot-water extract of Coptidis rhizome.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) The above objects, other features and advantages of the present invention will become more apparent by describing the preferred embodiments thereof with reference to the accompanying drawings, in which:

(2) FIG. 1 is a flow chart showing a process for manufacturing a wet tissue containing a hot-water extract of Coptidis rhizome according to the present invention; and

(3) FIG. 2 is a flow chart showing a process for manufacturing a wet tissue containing a distillate of hot-water extract of Coptidis rhizome according to the present invention.

PREFERRED EMBODIMENTS OF THE INVENTION

(4) Hereinafter, preferred examples of the present invention will be described in detail. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the examples set forth herein. Rather, these examples are provided so that this disclosure will be thorough and complete and will fully convey the concept of the invention to those skilled in the art.

Example 1: Preparation of Hot-Water Extract of Coptidis rhizome and Distillate Thereof

Examples 1-1 to 1-24: Hot-Water Extract of Coptidis rhizome

(5) According to the compositions shown in Table 1 below, Coptidis rhizome was mixed with water, and then extracted under the high temperature and high pressure conditions of 120-131 C. and 1.2-2.8 atm, thereby preparing hot-water extracts of Coptidis rhizome (a liquid phase remaining after removal of herbal solids after extraction was used, and herbal solids in the extracts in the Examples and the Comparative Examples were removed in the same manner). In addition, to enhance the antibacterial and antimicrobial effects of the hot-water extract of Coptidis rhizome, an extract of a mixture of Coptidis rhizome with at least one of Glycyrrhizae radix, Cassia obtusifolia L., Houttuyniae herba, Hagocho, Phellodendron bark, and Scutellaria root was prepared. Herein, the hot-water extract of Coptidis rhizome was prepared using a high-temperature and high-pressure extractor (Daerin Machinery Co., Ltd., Korea). Also, the volume of each of the extracts was adjusted to have the same volume as that of Example 1-1 by concentration or the addition of water.

(6) TABLE-US-00001 TABLE 1 Weight (g) Temp- Coptidis Glycyrrhizae Cassia Houttuyniae Scutellaria Phellodendron erature Pressure Time Conditions rhizoma radix obtusifolia L. herba Hagocho root bark water ( C.) (atm) (min) Ex. 1-1 100 5000 120 2.0 30 Ex. 1-2 100 2000 120 2.0 30 Ex. 1-3 100 8000 120 2.0 30 Ex. 1-4 100 5000 121 1.2 30 Ex. 1-5 100 5000 120 2.0 20 Ex. 1-6 100 5000 120 2.0 120 Ex. 1-7 90 10 5000 120 2.0 30 Ex. 1-8 90 10 5000 120 2.0 30 Ex. 1-9 90 10 5000 120 2.0 30 Ex. 1-10 80 20 5000 120 2.0 30 Ex. 1-11 80 20 5000 120 2.0 30 Ex. 1-12 80 20 5000 120 2.0 30 Ex. 1-13 80 20 5000 120 2.0 30 Ex. 1-14 80 10 10 5000 120 2.0 30 Ex. 1-15 80 10 10 5000 120 2.0 30 Ex. 1-16 80 10 10 5000 120 2.0 30 Ex. 1-17 70 10 10 10 5000 120 2.0 30 Ex. 1-18 70 30 5000 120 2.0 30 Ex. 1-19 70 30 5000 120 2.0 30 Ex. 1-20 70 30 5000 120 2.0 30 Ex. 1-21 80 20 5000 120 2.0 30 Ex. 1-22 80 20 5000 120 2.0 30 Ex. 1-23 80 10 10 5000 120 2.0 30 Ex. 1-24 100 5000 130 2.5 30

Examples 1-25 to 1-28: Distillate of Hot-Water Extract of Coptidis rhizome

Example 1-25

(7) Vapor obtained by distilling the extract of Example 1-1 at a temperature of 120-131 C. was condensed, thereby obtaining a distillate of hot-water extract of Coptidis rhizome.

Example 1-26

(8) Vapor obtained by distilling the extract of Example 1-8 at a temperature of 120-131 C. was condensed, thereby obtaining a distillate of hot-water extract of Coptidis rhizome.

Example 1-27

(9) Vapor obtained by distilling the extract of Example 1-9 at a temperature of 120-131 C. was condensed, thereby obtaining a distillate of hot-water extract of Coptidis rhizome.

Example 1-28

(10) Vapor obtained by distilling the extract of Example 1-13 at a temperature of 120-131 C. was condensed, thereby obtaining a distillate of hot-water extract of Coptidis rhizome.

Example 2: Manufacture of Wet Tissues Containing Hot-Water Extract of Coptidis rhizome Prepared Under High Temperature and High Pressure Conditions or Distillate Thereof

(11) 340 g of the extract or distillate of Example 1 was sprayed and absorbed onto 63 cotton tissue fabric sheets (16 cm18 cm; 100 g) (see Table 2). Next, the tissue treated with the extract or distillate was sealed and packaged, and then sterilized using a Hi-RETORT STERILIZER (Daerin Machinery Co., Ltd., Korea), thereby obtaining wet tissues (sterilized at 1.2 atm and 121 C. for 15 minutes).

(12) Meanwhile, wet tissues for oral cavity of Examples 2-7 and 2-10 were manufactured using an extract of a mixture of (Coptidis rhizome and Glycyrrhizae radix.

(13) TABLE-US-00002 TABLE 2 Wet tissue Extract used Example 2-1 Extract of Example 1-1 Example 2-2 Extract of Example 1-2 Example 2-3 Extract of Example 1-3 Example 2-4 Extract of Example 1-4 Example 2-5 Extract of Example 1-5 Example 2-6 Extract of Example 1-6 Example 2-7 Extract of Example 1-7 Example 2-8 Extract of Example 1-8 Example 2-9 Extract of Example 1-9 Example 2-10 Extract of Example 1-10 Example 2-11 Extract of Example 1-11 Example 2-12 Extract of Example 1-12 Example 2-13 Extract of Example 1-13 Example 2-14 Extract of Example 1-14 Example 2-15 Extract of Example 1-15 Example 2-16 Extract of Example 1-16 Example 2-17 Extract of Example 1-17 Example 2-18 Extract of Example 1-18 Example 2-19 Extract of Example 1-19 Example 2-20 Extract of Example 1-20 Example 2-21 Extract of Example 1-21 Example 2-22 Extract of Example 1-22 Example 2-23 Extract of Example 1-23 Example 2-24 Extract of Example 1-24 Example 2-25 Extract of Example 1-25 Example 2-26 Extract of Example 1-26 Example 2-27 Extract of Example 1-27 Example 2-28 Extract of Example 1-28

Example 3: Manufacture of Cotton Swab and Gauze Containing Hot-Water Extract of Coptidis rhizome

(14) 240 g of the hot-water extract of Coptidis rhizome of Example 1-1 was sprayed onto 100 g of each of cotton and cotton gauze fabric and dried. Then, a cotton swab was manufactured using the cotton, and the cotton gauze fabric was cut to a suitable size to thereby manufacture wound dressing gauze. Next, the cotton swab and the gauze were hermetically packaged, and then sterilized by irradiation with gamma-rays for 20 hours, thereby manufacturing final articles.

Example 4: Manufacture of Cotton Swab and Gauze Containing Distillate of Hot-Water Extract of Coptidis rhizome

(15) 240 g of the distillate of hot-water extract of Coptidis rhizome of Example 1-25 was sprayed onto 100 g of each of cotton and cotton gauze fabric and dried. Then, a cotton swab was manufactured using the cotton, and the cotton gauze fabric was cut to a suitable size to thereby manufacture wound dressing gauze. Next, the cotton swab and the gauze were hermetically packaged, and then sterilized by irradiation with gamma-rays for 20 hours, thereby manufacturing final articles.

Comparative Example 1: Preparation of Hot-Water Extracts of Coptidis rhizome for Comparison

(16) According to the compositions shown in Table 3 below, Coptidis rhizome was mixed and extracted, thereby preparing hot-water extracts of Coptidis rhizome for comparison. Also, to compare the effects of the extracts, the weight of each extract was adjusted to the same weight as that of each of the extracts of Example 1 by addition of water or concentration.

(17) TABLE-US-00003 TABLE 3 Weight (g) Temp- Coptidis Glycyrrhizae Cassia Houttuyniae Scutellaria Phellodendron erature Pressure Time Conditions rhizoma radix obtusifolia L. herba Hagocho root bark water ( C.) (atm) (min) Comp. Ex. 100 5000 100 1.0 30 1-1 Comp. Ex. 100 5000 90 1.0 30 1-2 Comp. Ex. 100 5000 80 1.0 30 1-3 Comp. Ex. 90 10 5000 100 1.0 30 1-4 Comp. Ex. 90 10 5000 100 1.0 30 1-5 Comp. Ex. 90 10 5000 100 1.0 30 1-6 Comp. Ex. 80 20 5000 100 1.0 30 1-7 Comp. Ex. 80 20 5000 100 1.0 30 1-8 Comp. Ex. 80 20 5000 100 1.0 30 1-9 Comp. Ex. 80 20 5000 100 1.0 30 1-10 Comp. Ex. 80 10 10 5000 100 1.0 30 1-11 Comp. Ex. 80 10 10 5000 100 1.0 30 1-12 Comp. Ex. 80 10 10 5000 100 1.0 30 1-13 Comp. Ex. 70 10 10 10 5000 100 1.0 30 1-14 Comp. Ex. 70 30 5000 100 1.0 30 1-15 Comp. Ex. 70 30 5000 100 1.0 30 1-16 Comp. Ex. 70 30 5000 100 1.0 30 1-17 Comp. Ex. 80 20 5000 100 1.0 30 1-18 Comp. Ex. 80 20 5000 100 1.0 30 1-19 Comp. Ex. 80 10 10 5000 100 1.0 30 1-20 Comp. Ex. 100 5000 120 2.0 30 1-21 Comp. Ex. 100 5000 120 2.0 30 1-22 Comp. Ex. 100 5000 120 2.0 30 1-23 Comp. Ex. 100 5000 120 2.0 30 1-24 Comp. Ex. 100 5000 120 2.0 30 1-25 Comp. Ex. 100 5000 120 2.0 30 1-26 Comp. Ex. 100 5000 95 1.5 30 1-27

Comparative Example 2: Manufacture of Wet Tissue Containing Organic Solvent Extract of Coptidis rhizome

(18) According to the compositions shown in Table 4 below, Coptidis rhizome extracts were prepared using 70% ethanol aqueous solution in place of water. Each of the extracts was filtered to remove herbal solids, and the filtrate was concentrated under reduced pressure to remove ethanol. Then, the weight of each extract was adjusted to the same weight as that of each of the extracts of Example 1 by addition of water.

(19) Meanwhile, extracts of Comparative Examples 2-8 and 2-9 were prepared in the following manner. Coptidis rhizome and 1,3-butyleneglycol or 70% ethanol solvent were placed in an extractor, which was then completely sealed so that pressure did not leak. The extraction of Coptidis rhizome was performed while the internal temperature of the extractor was elevated stepwise to 85 C. or 95 C. over 1 hour and maintained at that temperature for 3 hours. The extraction pressure was maintained at 1.2 atm or 1.5 atm. Also, to increase the efficiency of extraction, a stirrer having an anchor and a paddle was used, and the stirring speed during the extraction was maintained at 40-50 rpm. After completion of the extraction, the reactor was cooled to room temperature over 1 hour, and then the extract was filtered through a Nutsch filter to remove Coptidis rhizome solids. The filtrate was further filtered through filter paper (pore size: 1 m or less), and distilled under reduced pressure to remove the organic solvent. The volume of the distillate was adjusted to the same volume of each extract of Example 1 by adding water thereto.

(20) TABLE-US-00004 TABLE 4 Weight (g) Cassia Phello- 1,3 Temp- Coptidis Glycyrrhizae obtusifolia Houttuyniae Scutellaria dendron 70% butylene- erature Pressure Time Conditions rhizoma radix L. herba Hagocho root bark ethanol glycol ( C.) (atm) (min) Comp. Ex. 100 500 80 1.0 30 2-1 Comp. Ex. 80 20 500 80 1.0 30 2-2 Comp. Ex. 80 20 500 80 1.0 30 2-3 Comp. Ex. 80 20 500 80 1.0 30 2-4 Comp. Ex. 80 20 500 80 1.0 30 2-5 Comp. Ex. 80 20 500 80 1.0 30 2-6 Comp. Ex. 80 20 500 80 1.0 30 2-7 Comp. Ex. 100 500 85 1.2 240 2-8 Comp. Ex. 100 500 80 1.5 240 2-9

Comparative Example 3: Manufacture of Wet Tissues for Comparison

(21) Wet tissues were manufactured in the same manner as described in Example 2, except that the extract shown in Table 5 below was sprayed onto a fabric for wet tissue.

(22) TABLE-US-00005 TABLE 5 Wet tissue Extract used Comparative Example 3-1 Extract of Comparative Example 1-1 Comparative Example 3-2 Extract of Comparative Example 1-2 Comparative Example 3-3 Extract of Comparative Example 1-3 Comparative Example 3-4 Extract of Comparative Example 1-4 Comparative Example 3-5 Extract of Comparative Example 1-5 Comparative Example 3-6 Extract of Comparative Example 1-6 Comparative Example 3-7 Extract of Comparative Example 1-7 Comparative Example 3-8 Extract of Comparative Example 1-8 Comparative Example 3-9 Extract of Comparative Example 1-9 Comparative Example 3-10 Extract of Comparative Example 1-10 Comparative Example 3-11 Extract of Comparative Example 1-11 Comparative Example 3-12 Extract of Comparative Example 1-12 Comparative Example 3-13 Extract of Comparative Example 1-13 Comparative Example 3-14 Extract of Comparative Example 1-14 Comparative Example 3-15 Extract of Comparative Example 1-15 Comparative Example 3-16 Extract of Comparative Example 1-16 Comparative Example 3-17 Extract of Comparative Example 1-17 Comparative Example 3-18 Extract of Comparative Example 1-18 Comparative Example 3-19 Extract of Comparative Example 1-19 Comparative Example 3-20 Extract of Comparative Example 1-20 Comparative Example 3-21 Extract of Comparative Example 1-21 Comparative Example 3-22 Extract of Comparative Example 1-22 Comparative Example 3-23 Extract of Comparative Example 1-23 Comparative Example 3-24 Extract of Comparative Example 1-24 Comparative Example 3-25 Extract of Comparative Example 1-25 Comparative Example 3-26 Extract of Comparative Example 1-26 Comparative Example 3-27 Extract of Comparative Example 1-27 Comparative Example 3-28 Extract of Comparative Example 2-1 Comparative Example 3-29 Extract of Comparative Example 2-2 Comparative Example 3-30 Extract of Comparative Example 2-3 Comparative Example 3-31 Extract of Comparative Example 2-4 Comparative Example 3-32 Extract of Comparative Example 2-5 Comparative Example 3-33 Extract of Comparative Example 2-6 Comparative Example 3-34 Extract of Comparative Example 2-7 Comparative Example 3-35 Extract of Comparative Example 2-8 Comparative Example 3-36 Extract of Comparative Example 2-9 Comparative Example 3-37 Only water added

Comparative Example 4: Manufacture for Cotton Swab and Gauze Containing Hot-Water Extract of Coptidis rhizoma for Comparison

(23) A cotton swab and gauze containing the hot-water extract of Coptidis rhizome were manufactured in the same manner as described in Example 3, except that the hot-water extract of Coptidis rhizoma of Comparative Example 1-1 was sprayed.

Experimental Example 1: Examination of Long-Term Storage Stability

(24) The wet tissues of Example 2 and Comparative Example 3 were allowed to stand at room temperature (25 t) in a sealed and packaged state. At one month, the cover of the wet tissue package was opened, and 2 sheets of the tissue were taken out of the package and used in the experiment. Then, the cover of the wet tissue package was closed and the package was allowed to stand in the same manner as described above. At 3 months, two sheets of the tissue were taken out of the package and used in the experiment. At 6 months, two sheets of the tissue were finally taken out of the package and used in the experiment. Herein, the cover was carefully opened and closed so that the wet tissue would not be dried due to external conditions, and the package was stored in a closed space having a humidity of 80% or more.

(25) Using the wet tissues taken at each point of time, the degree of growth of general bacterial in the wet tissue during each storage period was examined. Each of the taken wet tissues was stirred in a stomacher bag containing peptone water for 120 seconds, and then serially diluted. 0.1 ml of each of the dilutions was plated on a plate count agar (PCA) plate medium. Measurement of general bacteria was performed using 3M Petrifilm after the dilution was incubated on the PCA medium at 35 C. for 24 hours. The results of the experiment are shown in Table 6 below. E. coli

(26) TABLE-US-00006 TABLE 6 Number of coli forms, log CFU/g Water tissue 1 month 3 months 6 months Example 2-1 ND ND ND Example 2-2 ND ND ND Example 2-3 ND ND ND Example 2-4 ND ND ND Example 2-5 ND ND ND Example 2-6 ND ND ND Example 2-7 ND ND ND Example 2-8 ND ND ND Example 2-9 ND ND ND Example 2-10 ND ND ND Example 2-11 ND ND ND Example 2-12 ND ND ND Example 2-13 ND ND ND Example 2-14 ND ND ND Example 2-15 ND ND ND Example 2-16 ND ND ND Example 2-17 ND ND ND Example 2-18 ND ND ND Example 2-19 ND ND ND Example 2-20 ND ND ND Example 2-21 ND ND ND Example 2-22 ND ND ND Example 2-23 ND ND ND Example 2-24 ND ND ND Example 2-25 ND ND ND Example 2-26 ND ND ND Example 2-27 ND ND ND Example 2-28 ND ND ND Comp. Ex. 3-1 ND 0.34 1.32 Comp. Ex. 3-2 ND 0.13 1.21 Comp. Ex. 3-3 ND 0.12 1.42 Comp. Ex. 3-4 ND 0.27 1.47 Comp. Ex. 3-5 ND 0.20 1.64 Comp. Ex. 3-6 ND 0.19 1.48 Comp. Ex. 3-7 ND 0.18 1.57 Comp. Ex. 3-8 ND 0.17 1.97 Comp. Ex. 3-9 ND 0.10 1.69 Comp. Ex. 3-10 ND 0.29 1.76 Comp. Ex. 3-11 ND 0.28 1.87 Comp. Ex. 3-12 ND 0.17 1.08 Comp. Ex. 3-13 ND 0.10 1.75 Comp. Ex. 3-14 ND 0.29 1.86 Comp. Ex. 3-15 ND 0.28 1.67 Comp. Ex. 3-16 ND 0.27 1.64 Comp. Ex. 3-17 ND 0.16 1.45 Comp. Ex. 3-18 ND 0.29 1.53 Comp. Ex. 3-19 ND 0.28 1.64 Comp. Ex. 3-20 ND 0.15 1.72 Comp. Ex. 3-21 ND 0.12 1.32 Comp. Ex. 3-22 ND 0.23 1.53 Comp. Ex. 3-23 ND 0.24 1.64 Comp. Ex. 3-24 ND 0.15 1.31 Comp. Ex. 3-25 ND 0.23 1.46 Comp. Ex. 3-26 ND 0.24 1.43 Comp. Ex. 3-27 ND 0.17 1 84 Comp. Ex. 3-28 ND 0.24 1.83 Comp. Ex. 3-29 ND 0.26 1.94 Comp. Ex. 3-30 ND 0.25 1.02 Comp. Ex. 3-31 ND 0.14 1.73 Comp. Ex. 3-32 ND 0.25 1.84 Comp. Ex. 3-33 ND 1.53 1.15 Comp. Ex. 3-34 ND 0.99 1.22 Comp. Ex. 3-35 ND 0.19 1.41 Comp. Ex. 3-36 ND 0.18 1.52 Comp. Ex. 3-37 ND 1.53 3.15 Commercially available Water tissue ND ND ND (Kleenex, Yuhan Kimberly Co., Ltd.) * ND: Not Detected

(27) As can be seen from the results in Table 6 above, the wet tissue of Comparative Example 3-37 containing only water did not inhibit the growth of general bacteria caused by external contamination during 6 months after manufacture, even though it was sterilized. In addition, in the case of the remaining wet tissues of Comparative Example 3, general bacteria caused by external contamination partially proliferated with the passage of time. However, in the case of the wet tissues of Example 2, it could be seen that general bacteria did not proliferate for 6 months, suggesting that the hot-water extract of Coptidis rhizoma according to the present invention has the effect of inhibiting external contamination without needing to add a preservative or an antibacterial agent, and thus the wet tissue containing it has high storage stability.

(28) Meanwhile, for the wet tissues of Examples 2-1 and 2-25 to 2-28, an experiment on the degree of growth of general bacteria in the wet tissues was performed for 18 months while measurement was performed at 3-month intervals. As a result, in the wet tissue of Example 2-1, 0.3 log CFU/g or more of bacteria were detected at 15 months (not detected up to 12 months), but in the wet tissues of Examples 2-25 to 2-28, 0.3 log CFU/g or more of bacteria were detected at 18 months (not detected up to 15 months).

(29) These results suggest that the wet tissue containing a distillate of the hot-water extract of Coptidis rhizoma has higher storage stability than the wet tissue containing the hot-water extract of Coptidis rhizome.

Example 2: Examination of Antibacterial Effect

(30) An experiment on the antibacterial effect of the wet tissue of the present invention against E. coli was performed. Specifically, 70 ml of phosphate buffered saline and 5 ml of a broth of Escherichia coli (accession No. ATCC 25922) were placed in a 250-ml flask, and 0.75 g of each of the wet tissues of Example 2 and Comparative Example 3 was cut to small pieces (11 cm or less) and added thereto. Each of the flasks was shaken at 320 rpm at 255 C. for 1 hour. Then, each of the cultures was diluted 10-fold with phosphate buffered saline, and then 0.1 ml of each dilution was streaked on agarose medium, after which each medium was incubated at 37 C. for 24 hours. The results are shown in Table 7 below as bacteria reduction ratio relative to the wet tissue of Comparative Example 3-37.

(31) TABLE-US-00007 TABLE 7 Water tissue Bacteria reduction ratio(%) Example 2-1 80.5 Example 2-2 85.8 Example 2-3 76.5 Example 2-4 77.6 Example 2-5 74.4 Example 2-6 85.5 Example 2-7 85.6 Example 2-8 94.7 Example 2-9 85.9 Example 2-10 93.0 Example 2-11 94.7 Example 2-12 95.8 Example 2-13 96.3 Example 2-14 93.4 Example 2-15 94.5 Example 2-16 95.2 Example 2-17 96.3 Example 2-18 97.4 Example 2-19 94.5 Example 2-20 95.3 Example 2-21 96.4 Example 2-22 94.5 Example 2-23 95.6 Example 2-24 95.6 Example 2-25 97.6 Example 2-26 97.7 Example 2-27 97.9 Example 2-28 97.6 Comp. Ex. 3-1 39.3 Comp. Ex. 3-2 36.4 Comp. Ex. 3-3 37.5 Comp. Ex. 3-4 24.4 Comp. Ex. 3-5 35.5 Comp. Ex. 3-6 46.7 Comp. Ex. 3-7 37.4 Comp. Ex. 3-8 44.5 Comp. Ex. 3-9 35.7 Comp. Ex. 3-10 46.8 Comp. Ex. 3-11 47.5 Comp. Ex. 3-12 44.6 Comp. Ex. 3-13 45.8 Comp. Ex. 3-14 33.9 Comp. Ex. 3-15 34.5 Comp. Ex. 3-16 35.6 Comp. Ex. 3-17 32.7 Comp. Ex. 3-18 33.4 Comp. Ex. 3-19 44.5 Comp. Ex. 3-20 45.6 Comp. Ex. 3-21 25.4 Comp. Ex. 3-22 23.5 Comp. Ex. 3-23 24.6 Comp. Ex. 3-24 26.7 Comp. Ex. 3-25 23.5 Comp. Ex. 3-26 22.8 Comp. Ex. 3-27 41.9 Comp. Ex. 3-28 46.3 Comp. Ex. 3-29 37.4 Comp. Ex. 3-30 34.5 Comp. Ex. 3-31 35.2 Comp. Ex. 3-32 37.3 Comp. Ex. 3-33 32.3 Comp. Ex. 3-34 33.7 Comp. Ex. 3-35 42.3 Comp. Ex. 3-36 41.4 Comp. Ex. 3-37 0.0 commercially available Water tissue 57.2 (Kleenex, Yuhan Kimberly Co., Ltd.)

(32) As can be seen from the results in Table 7 above, the medium containing the wet tissue product of the present invention showed a significant bacteria reduction ratio compared to the wet tissue of Comparative Example 3-37, suggesting that the wet tissue product of the present invention has a significantly high antibacterial activity compared to a commercially available wet tissue product. Meanwhile, the wet tissues (Comparative Examples 3-21 to 3-26) containing no Coptidis rhizoma extract had no antibacterial activity, suggesting that Coptidis rhizoma is essential for the antibacterial effect of the wet tissue of the present invention.

Experimental Example 3: Examination of Anti-Inflammatory Effect

(33) To examine the anti-inflammatory effect of the wet tissue of the present invention, bacterial conjunctivitis patient groups, each consisting of 10 persons, were allowed to wipe their upper eyelids using one sheet of the tissue of each of Example 2 and Comparative Example 3 at 6-hour intervals. Also, for an accurate comparative experiment, Fluorometholone Ophthalmic Suspension 0.1 (based on fluorometholone) that is a bacterial conjunctivitis therapeutic agent was administered at 6-hour intervals. The therapeutic effect of the wet tissue after 2 days of use was evaluated on a five-point scale, and the results of the evaluation are shown in Table 8.

(34) TABLE-US-00008 TABLE 8 Healing effect after Conditions 2 days of use Water tissue of Example 2-1 3.9 Water tissue of Example 2-2 3.8 Water tissue of Example 2-4 3.5 Water tissue of Example 2-11 4.9 Water tissue of Example 2-12 4.9 Water tissue of Example 2-13 4.7 Water tissue of Example 2-25 4.8 Water tissue of Example 2-26 4.8 Water tissue of Comp. Ex. 3-1 2.0 Water tissue of Comp. Ex. 3-3 1.8 Water tissue of Comp. Ex. 3-8 2.2 Water tissue of Comp. Ex. 3-10 2.1 Water tissue of Comp. Ex. 3-27 2.3 Water tissue of Comp. Ex. 3-35 2.2 Water tissue of Comp. Ex. 3-36 2.0 Water tissue of Comp. Ex. 3-37 1.1 Fluorometholone Ophthalmic Suspension 0.1 4.8 5: very good, 4: good, 3: moderate, 2: poor, 1: very poor

(35) As can be seen from the results in Table 8 above, the use of the wet tissues of Example 2 showed a therapeutic effect similar to that of the use of Fluorometholone Ophthalmic Suspension 0.1. In addition, it was shown that the wet tissues of Example 2 showed the effects of eliminating congestion, reducing inflammation, and inhibiting dry eye syndrome and pain.

Experimental Example 4: Examination of Effect on Inhibition of Oral Malodor

(36) 10 persons per group, who were in need of inhibition of oral malodor, were allowed to wipe their mouth cavity (including tongue) using one sheet of the tissue of each of Examples 2-7 and 2-10 (containing an extract prepared by extracting Coptidis rhizoma together with Glycyrrhizae radix in hot water), Comparative Examples 3-4 and 3-7, and Comparative Example 3-37 (treated with only water). The oral malodor inhibitory effect of the wet tissue after 2 days of use was evaluated on a five-point scale, and the results of the evaluation are shown in Table 9.

(37) TABLE-US-00009 TABLE 9 Healing effect after Conditions 2 days of use Water tissue of Example 2-7 4.1 Water tissue of Example 2-10 4.6 Water tissue of Comp. Ex. 3-4 2.3 Water tissue of Comp. Ex. 3-7 2.1 Water tissue of Comp. Ex. 3-37 1.1 5: very good, 4: good, 3: moderate, 2: poor, 1: very poor

(38) As can be seen from the results in Table 9 above, the use of the wet tissues of Examples 2-7 and 2-10 (containing an extract prepared by extracting Coptidis rhizoma together with Glycyrrhizae radix in hot water under the high temperature and high pressure conditions) exhibited an excellent effect on the inhibition of oral malodor. However, the wet tissue containing only water (Comparative Example 3-37) had no effect on the inhibition of oral malodor, and the wet tissues containing an extract prepared at 100 C. and 1 atm (Comparative Examples 3-4 and 3-7) showed an insignificant effect on the inhibition of oral malodor.

Experimental Example 5: Examination of Wound Healing Effect

(39) To examine the wound healing effect of the wet tissue of the present invention, on 10 persons per group, who had a wound having a length of 2-5 cm and a depth of 3 mm or less, the wound site was disinfected and dressed with the gauze of each of Examples 3 and 4 and Comparative Example 4. For comparison, general sterile gauze was used. After 24 hours, the gauze was replaced with fresh gauze, and after 24 hours, the degree of healing of the wound was evaluated on a five-point scale. The results of the evaluation are shown in Table below.

(40) TABLE-US-00010 TABLE 10 wound healing effect after Conditions 2 days of use gauze of Example 3 3.5 gauze of Example 4 4.1 Gauze of Comp. Ex. 4 2.5 commercially available general gauze 1.2 5: very good, 4: good, 3: moderate, 2: poor, 1: very poor

(41) As can be seen from the results in Table 10 above, the wound healing effect of the gauzes of Examples 3 and 4 was significantly better than that of the general gauze of Comparative Example 4. Particularly, the wound healing effect of the gauze of Example 4 containing the distillate of hot-water extract of Coptidis rhizoma was significantly better than that of the gauze of Example 3 containing the hot-water extract of Coptidis rhizome

(42) In addition, in the results of observation of the wound site, when the gauzes of Examples 3 and 4 were used, the wound was easily healed without forming a scab, suggesting that the gauzes showed good wound healing effects. However, the general gauze caused a severe scab, and showed little or no effect on wound healing.

Experimental Example 6: Examination of Skin Irritation of Coptidis rhizome Extract Using Closed Patch Test

(43) To examine the skin irritation of the hot-water extract of Coptidis rhizoma used in the wet tissue of the present invention, a human closed patch test was performed. This test method has been widely used to detect primary irritants. Specifically, 40 l of each of the extracts shown in Table 11 below was applied to the back of healthy adult persons (30 men and 30 women), and then fixed to the skin using a scanpore tape. After 24 hours, the tape was detached from the skin, and after 4 hours, the results were rated. The degree of erythema and edema was rated according to the following criteria. Criteria for evaluation followed the guidelines of the International Contact Dermatitis Research Group (ICDRG) (Wooding et al, 1967; Rietschell, 1982; Fischer & Maibach, 1984; Aberer et al, 1993) as follows:

(44) Criteria for Evaluation

(45) 0=no redness

(46) 1=mild erythema

(47) 2=intense erythema

(48) 3=intense erythema with edema

(49) 4=intense erythema with edema and vesicle