Polyelectrolyte multilayers having salt-controlled internal structures
09896593 ยท 2018-02-20
Assignee
Inventors
- Walter J. Dressick (Waldorf, MD, US)
- Kathryn J. Wahl (Alexandria, VA)
- Dmitri Y. Petrovykh (Braga, PT)
- Nabil D. Bassim (Silver Spring, MD, US)
- Rhonda Michelle Stroud (Washington, DC, US)
Cpc classification
Y10T428/31612
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C08K3/30
CHEMISTRY; METALLURGY
Y10T428/31663
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
International classification
Abstract
A method, and an article made therefrom, of: contacting a substrate with a first solution of first polyelectrolyte chains to form a layer of the first polyelectrolyte on the substrate; and contacting the layer of the first polyelectrolyte with a second solution of second polyelectrolyte chains to form a layer of the second polyelectrolyte. The first polyelectrolyte has a polyanion or polycation chain. The second polyelectrolyte has a polyanion or polycation chain of a charge opposite to that of the first polyelectrolyte. The first solution or the second solution is an aggregate-forming solution comprising an ionic species having at least two discrete sites of a charge opposite to that of the polyelectrolyte chains in the aggregate-forming solution. The ionic species forms, via bridging interactions, aggregates of the polyelectrolyte chains that remain intact in the aggregate-forming solution during the contact.
Claims
1. An article made by a method comprising: contacting a substrate with a first solution comprising first polyelectrolyte chains to form a layer of the first polyelectrolyte on the substrate; wherein the first polyelectrolyte comprises a polyanion or polycation chain; and contacting the layer of the first polyelectrolyte with a second solution comprising second polyelectrolyte chains to form a layer of the second polyelectrolyte on the layer of the first polyelectrolyte; wherein the second polyelectrolyte comprises a polyanion or polycation chain of a charge opposite to that of the first polyelectrolyte; wherein the first solution or the second solution is an aggregate-forming solution comprising an ionic species having at least two discrete sites of a charge opposite to that of the polyelectrolyte chains in the aggregate-forming solution; and wherein the ionic species forms, via bridging interactions, aggregates of the polyelectrolyte chains that remain intact in the aggregate-forming solution during the contact.
2. The article of claim 1, wherein the method further comprises: depositing on the layer of the second polyelectrolyte one or more alternating layers of the first polyelectrolyte using the first solution and the second polyelectrolyte using the second solution.
3. The article of claim 1; wherein the aggregate-forming solution comprises the polycation chain; and wherein the ionic species is an anion having two or more discrete spaced negatively-charged sites at an obtuse angle.
4. The article of claim 3, wherein the method forms a multilayer structure having internal pores and comprised of layers of stacked polycation aggregates separated and held together by layers of polyanion.
5. The article of claim 3, wherein the polycation chain is polyallylamine hydrochloride.
6. The article of claim 3, wherein the polycation chain is polydiallyldimethylammonium chloride.
7. The article of claim 3, wherein the ionic species is SO.sub.4.sup.2, HPO.sub.4.sup.2, or an organic dicarboxylate.
8. The article of claim 3, wherein the polyanion chain is sodium polystyrene sulfonate.
9. The article of claim 3, wherein the aggregate-forming solution is the second solution.
10. The article of claim 3, wherein the substrate is a silicon or quartz substrate having a self-assembled monolayer of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane.
11. The article of claim 1, wherein the method further comprises: rinsing the layer formed from the aggregate-forming solution with, in order: 1) a polyelectrolyte-free solution having the same salt composition as the aggregate-forming solution; 2) a 1.0 M NaCl solution; and 3) water.
12. The article of claim 1, further comprising: rinsing the layer formed from the aggregate-forming solution with water three times.
13. The article of claim 5, further comprising: depositing Na.sub.4Ru(BPS).sub.3 on a layer of polyallylamine hydrochloride.
Description
DESCRIPTION OF THE DRAWINGS
(1) A more complete appreciation of the invention will be readily obtained by reference to the following Description of the Example Embodiments and the accompanying drawings.
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DETAILED DESCRIPTION OF EXAMPLE EMBODIMENTS
(15) In the following description, for purposes of explanation and not limitation, specific details are set forth in order to provide a thorough understanding of the present disclosure. It will be apparent to one skilled in the art, however, that the present subject matter may be practiced in other embodiments that depart from these specific details. In other instances, detailed descriptions of well-known methods and devices are omitted, to avoid obscuring the present disclosure with unnecessary details.
(16) Disclosed herein is an alternate method of controlling the internal structure of polyelectrolyte multilayers whereby anions capable of electrostatically bridging cationic sites on polycation chains in solution organize said polycation chains into solution aggregates. Polycation aggregate size and solution concentration are tuned by the concentration and nature of the bridging anion present in the polycation deposition solution. The anion-bridged aggregates of polycation are sufficiently stable such that they adsorb relatively intact onto the polyanion terminal layer of a growing multilayer. The adsorbed aggregates control the surface morphology of the stratum that they comprise by virtue of their size (i.e., steric effects) and charge (i.e., electrostatic effects), which determine their surface coverage and spatial distribution at the film-solution interface. Spaces between adjacent adsorbed aggregates constitute defect sites, which are covered by subsequent adsorption of the next polyanion layer as film growth proceeds. As a result, a multilayer film having a controllable unique porous internal defect structure is fabricated. The bridging-anion salts may be readily removed from the adsorbed polycation aggregates by the rinsing process used during film fabrication. Therefore, the fabrication of the internal structure does not require the use of harsh chemical dissolution steps associated with the hard templates currently used to prepare similar films.
(17) The polyelectrolyte multilayer films may exhibit controllable, unique, internal defect structures and morphologies if the salt of an anion having two or more discrete negatively-charged sites, closely spaced and rigidly fixed at an obtuse angle with respect to each other, is present in the polyelectrolyte solutions during multilayer deposition. Specifically, anions having at least two discrete negatively-charged sites can electrostatically interact with oppositely charged sites on polycation chains, leading to intrachain and interchain bridging. Aggregate structures are formed via bridging anion interactions with the polycation in solution and these aggregates are sufficiently stable that they are adsorbed relatively intact onto the oppositely charged polyanion layer already present on the substrate surface during multilayer fabrication.
(18) In contrast univalent anions are unable to establish such bridging interactions with the polycation chains in solution. Univalent anions, however, can electrostatically bind to individual cationic sites on the polycation chain. This binding partially neutralizes the charge of the polycation chain. As a result, electrostatic repulsion among the positively-charged sites on the chain is also reduced, permitting weaker van der Waals and hydrogen bonding interactions to increasingly dictate chain conformation. These weaker interactions lead to chain coiling and weak association of polyelectrolyte chains in solution, resulting in the formation of loosely aggregated, fluxional polyelectrolyte species. Because such aggregates are weakly associated, they do not maintain any portion of their solution structure upon adsorption to the surface, unlike the more stable aggregates formed using the bridging anion species. An analogous argument applies to polyanion aggregates formed via associations between the polyanion and univalent cations (or non-bridging multivalent cations) in the solutions.
(19) Because the polycation aggregates formed via bridging anions adsorb relatively intact, their presence on the surface can impede and control further adsorption of aggregates from solution due to like-charge and steric repulsion effects. Therefore, defect or void sites are formed on the surface during polycation aggregate adsorption. Subsequent adsorption of polyanion aggregates from solution onto these polycation aggregates readily occurs. However, the loose, weakly interacting polyanion aggregates present in solutions cannot maintain their solution structure during the adsorption process. Consequently, they are sufficiently fluxional following adsorption to the polycation aggregates on the surface that they can (partially or fully) cover the void or defect sites present in the underlying polycation aggregate layer. As a result, the surface is healed as increasing numbers of polycation and polyanion layers are deposited during multilayer fabrication. This deposition process leads to a multilayer having an internal structure comprised of layers of stacked polycation aggregates separated and held together by layers of polyanion strands, creating a unique internal porosity.
(20) Bridging multivalent anions that are useful for stabilizing polycation aggregate structures in solution sufficiently well to permit their relatively intact adsorption onto the substrate during multilayer fabrication include, but are not limited to, sulfate, monohydrogen phosphate, phosphate, and various organic dicarboxylates, such as oxalate, maleate, tartrate, malonate, fumarate, and succinate (
(21) The use of bridging multivalent anions that are the most capable of stabilizing the polycation solution aggregates during multilayer deposition, such as sulfate or monohydrogen phosphate, yields multilayers having the most and largest polycation aggregates adsorbed to the film during deposition of each polycation layer. This permits some control of the numbers and sizes of the defect sites creating the internal structure of the multilayer film. In addition, the use of bridging multivalent anions leads to control of multilayer film thickness, roughness, and related properties. Consequently, the concentration and nature of the bridging multivalent anion present in the polyelectrolyte solutions during multilayer deposition permits tuning of multilayer structure and properties.
(22) There is no valid reason that analogously structured multilayer films from polyanions using bridging cations could not or would not exist, provided that an appropriate bridging cation can be found. Small, multivalent rigid cations having discrete cationic sites closely and rigidly spaced apart from each other at a fixed obtuse angle are rare compared to analogous bridging anions. However, there are a few organic molecules that may be adequate for this purpose. Some possible candidates would include sufficiently water-soluble multiamine containing materials such as ethylenediamine or melamine, as well as various methyl viologen isomers having the proper non-linear orientation of their alkylated N sites and transition metal complexes whose ligands have cationic sites fixed to their peripheries.
(23) The method disclosed herein permits fabrication of multilayer films having unique porous internal defect structures and film morphologies simply via choice and concentration of the multivalent bridging salt anion present in the polyelectrolyte solutions during multilayer deposition. Compared to current approaches requiring the use of a dissolution step to remove hard templates used for fabrication of similar films, the method offers at least two potential advantages. First, the use of added salts containing bridging anions to control polycation solution aggregation, and therefore ultimately internal multilayer structure, requires no use of harsh chemical agents to dissolve the template. This is an advantage for multilayer films which may have functional groups sensitive to the acids, bases, or other dissolution agent often used for this purpose. One such example would be a multilayer in which a polypeptide is present as one of the polyelectrolyte components. Hydrolysis of the polypeptide amide bonds by acid or base would degrade or destroy the polypeptide strata, and therefore also the multilayer film. Second, the bridging anion salt is readily removed during the standard rinsing protocol used to fabricate the multilayer films. If the presence of the bridging anion in the completed multilayer is desired, it is readily accomplished by modification of the rinse procedures used during film fabrication.
(24) Given their unique internal structures, the films may be useful for filtration and encapsulation applications. In addition, the use of the internal defect sites as nanoreactors for conducting chemical reactions within their ultra-small volumes inside the multilayer film is also possible. Because there is no known analogous method available for providing such a controllable unique internal defect structure within a polyelectrolyte multilayer film, the films are expected to find great use in these areas.
(25) The simplest model for PSS/PAH multilayer growth, derived from observations in solutions with SO.sub.4.sup.2 (but generalizable to other bridging anions), is illustrated in
(26) During the salt rinsing steps, excess PAH solution remaining at the film surface is first replaced by a corresponding polyelectrolyte-free mixed SO.sub.4.sup.2/Cl.sup. salt solution. The following rinse with 1.00 M NaCl (aq) solution exchanges SO.sub.4.sup.2 dianions in the adsorbed PAH aggregates for Cl.sup. anions, concomitantly reducing the hydration of the aggregates, because SO.sub.4.sup.2 is a kosmotropic anion that binds water well, and forcing partial deprotonation of the PAH amine sites to maintain electroneutrality (
(27) During a subsequent rinse in water, excess NaCl is removed from the surface. PAH chains near the surface of the aggregate respond to the decrease in solution ionic strength by partial uncoiling and extension into the solution, consistently with previous observations and with the two apparent populations of PAH chains observed in the Ru(BPS).sub.3.sup.4 dye experiments described herein. After the aqueous rinsing step, the tightly packed and intertwined chains in the interior of PAH aggregates are covered by more loosely packed and extended (re)protonated exterior chains exhibiting a more open structure. The presence of flexible exterior PAH chains after water rinsing facilitates healing of void defects as film thickness grows (
(28) Two important quantitative consequences of the anion-bridging model directly explain the unusual combination of exponential and linear features of the PSS/PAH film formation in the presence of SO.sub.4.sup.2 anions. First, higher levels of SO.sub.4.sup.2 in the PAH treatment solution will lead to increased aggregation and to deposition of more and larger PAH aggregates (
(29) The effects of univalent anions on PEMLs are successfully predicted by their ordering in the Hofmeister series (Hofmeister, Zur Lehre von der Wirkung der SalzeZweite Mittheilung Arch. Exp. Pathol. Pharmakol. (Leipzig) 1888, 24, 247-260). Accordingly, the Hofmeister effect was considered as an alternative explanation for the results in experiments with multivalent anions (
(30) In summary, the presence of bridging dianions as additives to PSS and PAH polyelectrolyte solutions used for the fabrication of PSS/PAH PEMLs may exert a profound influence on the physicochemical properties of the assembled multilayer films. In contrast to previous observations for univalent anion additives, whereby PSS/PAH film properties were related to anion position in the Hofmeister series, the tandem increases in film absorbance and thickness observed here are correlated to the dianion concentrations and their ability to bridge protonated amine sites of the PAH component of multilayers. Detailed structural analysis of films prepared using divalent sulfate anion as an additive indicates that polyelectrolyte deposition using the protocol proceeds in the linear regime and without inclusion of the dianion, yielding stratified film structures that comprise PSS-decorated PAH aggregates.
(31) The film deposition is well-described by a general model in which electrostatic bridging of protonated amine sites on the PAH chains by the dianions occurs in solution, leading to the formation of PAH aggregates that are adsorbed intact onto each terminal PSS layer in the growing film. This behavior contrasts with analogous PAH aggregates present in solutions containing univalent anions, such as chloride, where PAH aggregates are apparently more loosely organized and insufficiently stable to maintain their structure upon adsorption to the surface. As a result, films deposited from solutions containing bridging dianions experience packing inefficiencies, which lead to formation of void defects that are partially healed as additional polyelectrolyte layers are deposited. The repeated formation and healing of void defects creates a uniquely structured internally porous architecture. Therefore, the ability to control PAH aggregate size and concentration in the deposition solution by altering the nature and concentration of dianion present suggests the possibility for tuning internal structure to create films exhibiting controlled porous structures useful for diverse applications ranging from encapsulation and filtration to nanoscale reaction vessels.
(32) In the method disclosed herein, a substrate is provided, which may be any substrate on which a PEML may be deposited. Suitable substrates include, but are not limited to, a silicon or quartz substrate, which may have a self-assembled monolayer (SAM) of an organosiloxane, such as N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (EDA), thereon, providing initial cationic sites in aqueous solution (pH<9-10) for polyelectrolyte adsorption.
(33) Next, a first solution of first polyelectrolyte chains is deposited on the substrate to form a layer of the first polyelectrolyte. The first polyelectrolyte may be either anionic or cationic, depending on the charge of the substrate surface. For example, an anionic polyelectrolyte solution would be used to treat a substrate bearing an EDA SAM. Then a second solution of second polyelectrolyte is deposited on the layer of the first polyelectrolyte to form a layer of the second polyelectrolyte. The second polyelectrolyte is a polyanion or polycation chain of the opposite charge as the first polyelectrolyte. Suitable polyelectrolytes include, but are not limited to, polyallylamine hydrochloride (PAH), polydiallyldimethylammonium chloride (PDDA), and sodium polystyrene sulfonate (PSS). Depositing the polyelectrolyte layers may be repeated to form thicker PEMLs by depositing onto the layer of the second polyelectrolyte one or more alternating layers of the first polyelectrolyte using the first solution and the second polyelectrolyte using the second solution.
(34) One of the solutions, which may be the first solution or the second solution and may be anionic or cationic, is an aggregate-forming solution comprising an ionic species having at least two discrete sites of charge opposite to that of the polyelectrolyte chains in the aggregate-forming solution. As described herein the ionic species forms via bridging interactions aggregates of the polyelectrolyte chains in the aggregate-forming solution that remain intact during the deposition. This process can form a multilayer structure having internal pores and comprised of layers of stacked polycation (polyanion) aggregates separated and held together by layers of polyanion (polycation). Suitable ionic species include, but are not limited to, an anion having two or more discrete negatively-charged sites at an obtuse angle, SO.sub.4.sup.2, HPO.sub.4.sup.2, and an organic dicarboxylate (
(35) The PEML may be further processed by rinsing the layer formed from the aggregate-forming solution as described herein with 1) a polyelectrolyte-free solution having the same salt composition as the aggregate-forming solution, 2) a 1.0 M NaCl solution, and 3) water. Alternatively the layer formed from the aggregate-forming solution may be rinsed with water three times.
(36) The following examples are given to illustrate specific applications. These specific examples are not intended to limit the scope of the disclosure in this application.
Example 1
Materials
(37) Deionized water (18 M-cm) for all experiments was obtained from an Elix 5 Milli-Q Plus Ultra-Pure Water System (Millipore Corp.). All organic solvents were ACS reagent Grade or better used as received from Sigma-Aldrich. Nitrogen gas was obtained from liquid N.sub.2 boil-off via plumbed in-house lines and passed through a cellulose filter prior to use.
(38) The following materials were used as received from the indicated commercial source, unless noted otherwise. Polyallylamine hydrochloride (PAH, M.sub.w=70,000 g.Math.mole.sup.1, No. 28322-3), sodium polystyrenesulfonate (PSS, M.sub.w=70,000 g.Math.mole.sup.1, No. 24305-1), sodium chloride (NaCl, 99.5%, No. S7653), sodium dihydrogen phosphate (NaH.sub.2PO.sub.4, >99.0%, No. S8282), sodium fluoride (NaF, 99+%, No. 20, 115-4), glacial acetic acid (HOAc, 99.99+%, No. 338826), sodium acetate (NaC.sub.2H.sub.3O.sub.2, >99.0%, No. S8750), disodium hydrogen phosphate (Na.sub.2HPO.sub.4, 98%, anhydrous, No. S-7907), sodium oxalate (Na.sub.2C.sub.2O.sub.4, 99.99%, No. 379735), disodium fumarate (Na.sub.2C.sub.4H.sub.2N.sub.2O.sub.4, >99%, No. F1506), sodium sulfate (Na.sub.2SO.sub.4, 99%, No. 23, 391-3), magnesium chloride (MgCl.sub.2.6H.sub.2O, >99.0%, No. M0250), ruthenium trichloride hydrate (RuCl.sub.3.H.sub.2O, >98%, No. 206229), disodium bathophenanthrolinesulfonate (BPS, 98%, Na.sub.2C.sub.24H.sub.14N.sub.2S.sub.2O.sub.6.3H.sub.2O, No. 14661-7), and 2,2-bipyridine (bpy, C.sub.10H.sub.8N.sub.2, 99+%, No. D21630-5) were all from Sigma-Aldrich Chemicals. Magnesium sulfate (MgSO.sub.4, 98.0%, No. BDH0246) was from BCH Chemicals Inc. and L-(+)-sodium tartrate (Na.sub.2C.sub.4H.sub.4O.sub.6.2H.sub.2O, >99.0%, No. BP352) was from Fisher Scientific. Disodium malonate (Na.sub.2C.sub.3H.sub.2O.sub.4, 99%, No. M0031), disodium succinate (Na.sub.2C.sub.4H.sub.4O.sub.4.6H.sub.2O, >98.0%, S0105), and disodium maleate (Na.sub.2C.sub.4H.sub.2N.sub.2O.sub.4, >99.0%, No. M0014) were from TCI America Inc. N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (EDA, >95%, No. SIA0591.0) from Gelest Inc. was purified by vacuum distillation (140-142 C.; 14-15 mm Hg) immediately prior to use.
(39) Ru(bpy).sub.3(ClO.sub.4).sub.2 was initially prepared as the chloride salt by refluxing stoichiometric amounts of RuCl.sub.3 and bpy ligand in 1:1 v/v ethanol:water under N.sub.2 atmosphere for ca. 4 h. The crude product was isolated by evaporation of the solvent, dissolved in a minimum amount of water, and purified by ion exchange chromatography using a literature method (Huang et al., Luminescent -Diimine Complexes of Ruthenium(II) Containing Scorpionate Ligands Inorg. Chim. Acta 2000, 310, 227-236), followed by metathesis to the ClO.sub.4.sup. salt prior to use. Na.sub.4Ru(BPS).sub.3 was prepared from RuCl.sub.3 and BPS by the literature method (Zanarini et al., Electrochemistry and Electrochemiluminescence of [Ru(II)-tris(bathophenanthroline-disulfonate)].sup.4 in Aprotic Conditions and Aqueous Buffers J. Phys. Chem. B 2008, 112, 10188-10193).
(40) Polished quartz slides (No. 20200, 25.4 mm50.8 mm1 mm) were from Quartz Scientific Inc. Silicon wafers (100 mm diameter, 500-550 m thickness, [100] orientation, p-type (B doping), resistivity 6-9, No. PD7403) from Wacker Siltronic Corp. were cut into 2550 mm.sup.2 pieces for use in the experiments.
Example 2
Polyelectrolyte Solutions
(41) The deposition solutions were prepared by addition of concentrated aqueous salt mixtures to a well-stirred solution of PAH (or PSS) in water. Each salt mixture contained NaCl and the ion to be studied at concentrations that produced 0.01-0.05 M of the studied ion and =1.00 M ionic strength after mixing with the PAH (or PSS) solution (Table 1). The concentrations of PAH and PSS after mixing were each 2 mg/mL. Before mixing, levels of the ion to be studied were typically ca. 0.070-0.075 M, limited for some of the dianions by solubility or irreversible gelation upon mixing with PAH. Even at these ion concentrations, gels were irreversibly formed if PAH solution was added to some salt mixtures. Adding the salt mixture to a stirred PAH solution sometimes initially generated a white precipitate, which quickly dissolved, however, to form a clear stable solution. In contrast, no gelation was observed for PSS solutions.
(42) TABLE-US-00001 TABLE 1 Salt Solution Compositions and pH Values Solution pH.sup.c Salt Identity.sup.a Solution Composition.sup.b Rinse.sup.d PSS.sup.e PAH.sup.f NaCl 1.00M NaCl 6.50.sup.g 6.47 4.58 NaF 0.05M NaF/0.95M NaCl 7.35.sup.h 7.46.sup.h 5.75 NaH.sub.2PO.sub.4 0.05M NaH.sub.2PO.sub.4/0.95M NaCl 4.18 4.20 4.03 Na(Acetate) 0.05M H.sub.3CCOONa/0.95M NaCl 7.11 6.97 6.50 MgCl.sub.2 0.05M MgCl.sub.2/0.85M NaCl 6.77 6.46 5.14 MgSO.sub.4 0.05M MgSO.sub.4/0.80M NaCl 6.52 6.40 5.09 Na.sub.2SO.sub.4 0.05M Na.sub.2SO.sub.4/0.85M NaCl 6.97 6.91 6.05 Na.sub.2HPO.sub.4 0.011M Na.sub.2HPO.sub.4/0.010M NaH.sub.2PO4/ 6.25 6.25 6.15 0.967M NaCl Na.sub.2Oxalate 0.05M Na.sub.2C.sub.2O.sub.4/0.85M NaCl 7.57.sup.h 7.39.sup.h 6.40 Na.sub.2Tartrate 0.05M NaOOCCH(OH)CH(OH)COONa/ 6.68 6.67 6.05 0.85M NaCl Na.sub.2Maleate 0.05M cis-NaOOCCHCHCOONa/0.85M NaCl 8.12.sup.h 7.69.sup.h 7.19 Na.sub.2Fumarate 0.05M trans-NaOOCCHCHCOONa/0.85M NaCl 7.00 6.72 6.07 Na.sub.2Malonate 0.05M NaOOCCH.sub.2COONa/0.85M NaCl 7.42 7.28 6.86 Na.sub.2Succinate 0.05M NaOOC(CH.sub.2).sub.2COONa/0.85M NaCl 7.17 7.28 6.85 .sup.aIdentity of the unique salt present in each polyelectrolyte solution during the deposition of the multilayer films. .sup.bComposition of the salt solution showing the concentrations of the unique salt tested and added NaCl to adjust total solution ionic strength = 1.00M. .sup.cpH of freshly prepared solution measured at 23 2 C. Std. dev. = 0.03 pH units (from 3 measurements). .sup.dpH of the first multilayer film rinse solution having the composition shown in column 2 and used in the salt rinse protocol. .sup.epH of the solution shown in column 2 also containing 2 mg PSS polyelectrolyte/mL solution and used for multilayer deposition. .sup.fpH of the solution shown in column 2 also containing 2 mg PAH polyelectrolyte/mL solution and used for multilayer deposition. .sup.g1.00M NaCl (aq) solution having this pH is also used as the second rinse solution in the salt rinse Protocol. .sup.hSolution pH dropped ca. 0.1-0.3 units after repeated solution exposure to the air during normal use due to slow adsorption of carbon dioxide.
(43) The presence of SO.sub.4.sup.2 and related dianions in solution during deposition of multilayers containing PAH greatly limits the available process window for film fabrication. For example, the dianion concentration must be kept below its gelation threshold value to provide stable polyelectrolyte solutions required for reproducible film deposition. In particular, for preparation of the =1.00 M PAH solutions with the various salts used in this work, it was found that gel formation is inhibited by addition of a concentrated salt solution containing NaCl and a salt of the dianion to be studied to a well-stirred solution of PAH in water. Concentrations of components are chosen with regard to solubility considerations and gelation threshold, as described in this work and the available literature. The procedure produces stable solutions having appropriate levels of the dianion, NaCl, and PAH for PEML fabrication (Table 1). In contrast, addition of the PAH solution to the concentrated salt solution leads to immediate or eventual (within ca. 1-2 days) gelation for several dianions studied.
(44) The following example describes a typical preparation of a polyelectrolyte solution for fabrication of PEMLs. To prepare the PAH deposition solution containing 2 mg PAH/mL 0.05 M Na.sub.2SO.sub.4/0.85 M NaCl, 350 mL of a solution containing 0.071 M Na.sub.2SO.sub.4 and 1.210 M NaCl was slowly added with stirring to an aqueous solution prepared by dissolving 1.00 g PAH in 150 mL water. Although some white cloudiness was observed during the initial stages of the addition, the solution became clear as the addition progressed. The solution was stirred for an additional 30 min and stored sealed in a Pyrex bottle for 2 days prior to use. Other solutions listed in Table 1 were prepared in a similar manner.
(45) Finally, because PAH contains primary alkylamine groups, solution pH must also be controlled to ensure that the degree of amine protonation remains approximately constant during the PEML deposition process regardless of which salt is present in solution. Kobayashi et al. (Poly(allylamine)-Chelating Properties and Resins for Uranium Recovery from Seawater Macromolecules 1985, 18, 2357-2361) have measured a pK.sub.a.sup.=19.67 for PAH in =1.00 M KCl (aq) solution and confirmed that it acts as a monobasic species. This value is ca. 0.9-1.0 pK unit lower than that expected for an aliphatic primary amine in aqueous solution (.fwdarw.0), consistent with independent measurements of the general effect of on pK.sub.a values in the solutions. Therefore, to maintain complete (i.e., >99%) protonation of the alkylamine sites of PAH in solution during PEML deposition, the PSS and PAH solutions must have pH7.67. This requirement is met for all experiments (note Table 1), ensuring consistent protonation of the PAH during multilayer fabrication regardless of the nature of the salt present.
Example 3
Substrate Preparation
(46) Silicon wafer and quartz substrates were cleaned with 1:1 v/v HCl/CH.sub.3OH and H.sub.2SO.sub.4, as described in Dressick et al., Proximity X-ray Lithography of Siloxane and Polymer Films Containing Benzyl Chloride Functional Groups J. Vac. Sci. Technol., A 1999, 17, 1432-1440. Thereafter, a self-assembled monolayer (SAM) of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (EDA) was immediately formed on each substrate as described in Chen et al. Channel-constrained Electroless Metal Deposition on Ligating Self-Assembled Film Surfaces J. Electrochem. Soc. 1999, 146, 1421-1430. The EDA-coated Si wafers (Si-EDA) and quartz (Q-EDA) were stored in Fluoroware containers and were sufficiently stable and clean for PEML deposition experiments for at least 6 months after preparation.
Example 4
Polyelectrolyte Deposition
(47) The PSS/PAH multilayer films were prepared on the lab bench under ambient conditions by dipcoating substrates in the PAH and PSS polyelectrolyte solutions containing the appropriate salts (
(48) TABLE-US-00002 TABLE 2 Properties of Multilayer Films Deposited from Salt Solutions (Triple Water Rinse Protocol) thickness (nm) roughness (nm) salt.sup.a (substrate).sup.b absorbance.sup.c total film.sup.d bilayer.sup.e r.sub.q.sup.f N.sup.g NaCl.sup.h (Q) 0.7588 NaCl.sup.h (Si) 97.5 2.2 4.1 0.1 0.83 0.37 3 NaH.sub.2PO.sub.4.sup.h (Q) 0.7696 80.4 1.6 4.0 0.1 0.95 0.12 5 NaH.sub.2PO.sub.4.sup.h (Si) 100.5 0.7 4.2 0.3 0.84 0.09 3 MgCl.sub.2.sup.i (Q) 0.7466 77.4 7.2 3.9 0.4 1.27 0.16 3 MgCl.sub.2.sup.i (Si) 93.5 4.0 3.9 0.1 0.99 0.50 4 NaSO.sub.4.sup.i (Q) 2.8157 347.6 12.5 17.4 0.6 15.90 4.80 3 NaSO.sub.4.sup.i (Si) 411.6 13.6 17.1 0.4 MgSO.sub.4.sup.i (Q) 2.6300 382.0 50.0 19.1 2.5 35.55 5.88 4 Na.sub.2HPO.sub.4.sup.k (Si) 304.9 5.5 12.7 0.2 2.91 0.68 3 .sup.aIdentity of the univalent or divalent ion salt present in each polyelectrolyte solution during multilayer deposition. Each salt was present at 0.05M concentration, except where noted, with sufficient NaCl added to fix the solution ionic strength at = 1.00M. .sup.bIdentity of the substrate coated with the multilayer. Q = quartz and Si = silicon wafer. Substrates were coated with a chemisorbed EDA organosiloxane monolayer prior to multilayer deposition. Multilayer films have structure: Substrate-EDA/(PSS/PAH).sub.x (x = 20 for quartz (Q) and x = 24 for silicon (Si) substrates). Films were triple-rinsed with water and dried in the filtered N.sub.2 gas stream after deposition of each PSS and PAH layer. .sup.cUV absorbance of the multilayer film at 225 nm on a quartz substrate. .sup.dThickness (std. dev.) of the multilayer film as determined by AFM. .sup.eAverage thickness (std. dev.) of a single PSS/PAH bilayer within the multilayer film, calculated by division of the total film thickness by the number of bilayers deposited, x, with x = 20 (quartz) or x = 24 (Si). .sup.fRoot-mean-square roughness, r.sub.q, (std. dev.) from AFM measurements. .sup.gNumber of measurements taken to determine roughness values. .sup.hContains 0.95M NaCl added to adjust ionic strength. .sup.IContains 0.85M NaCl added to adjust ionic strength. .sup.jContains 0.80M NaCl added to adjust ionic strength. .sup.kPAH solubility issues limit the Na.sub.2HPO.sub.4 concentration to 0.011M, with 0.957M NaCl and with 0.010M NaH.sub.2PO.sub.4 added for ionic strength and pH buffer control.
(49) For PEMLs containing the Na.sub.4Ru(BPS).sub.3 complex of structure shown in
(50) HPO.sub.4.sup.2 dianion was used to provide a comparison with the results for SO.sub.4.sup.2 because both these anions possess two angled anionic sites (PO.sup. and SO.sup., respectively, see
(51) To develop a better understanding of the PEML deposition in presence of phosphate, its ionization was examined under the experimental conditions. For the reaction:
H.sub.2PO.sub.4.sup.H.sup.++HPO.sub.4.sup.2(1a)
pK.sub.a=pH+log [HPO.sub.4.sup.2]/[H.sub.2PO.sub.4.sup.](1b)
If [HPO.sub.4.sup.2]=[H.sub.2PO.sub.4.sup.], the logarithm term vanishes and pK.sub.a=pH. Therefore, a =1.00 M solution containing 0.958 M NaCl, 0.011 M NaH.sub.2PO.sub.4, and 0.011 M Na.sub.2HPO.sub.4 was prepared, and its pH=pK.sub.a=6.290.03 measured. This value is ca. 0.9 pK units lower than the pK.sub.a=7.210.01 measured in water for the same reaction (CRC Handbook of Chemistry and Physics, 79th Ed.; Lide, D. R., Ed.; CRC Press: New York, 1999; p. 8-45).
(52) Given the solution pH of the experiments (cf., Tables 1 and 3), the third POH site of HPO.sub.4.sup.2 will exist in the non-ionized POH form in solution in the absence of PAH. The superior performance of HPO.sub.4.sup.2 in PEML deposition experiments may be a consequence of the potential deprotonation of that third POH site. Electrostatic binding of the two PO.sup. sites to protonated amines on the polyelectrolyte chain in PAH solution will alter the local environment of the remaining POH site. Spontaneous deprotonation of the remaining POH site would permit formation of an additional electrostatic interaction with an adjacent PAH protonated amine, further stabilizing the (PAH-NH.sub.3.sup.+).sub.2 . . . HPO.sub.4.sup.2 interface as shown in Eq. (2):
(PAH-NH.sub.3.sup.+).sub.2 . . . HPO.sub.4.sup.2+PNH.sub.3.sup.+.fwdarw.H.sup.++(PNH.sub.3.sup.+).sub.3 . . . PO.sub.4.sup.3 (where PNH.sub.3.sup.+ is PAH)(2)
The extreme pH sensitivity of the HPO.sub.4.sup.2 bridging phenomenon, which is quenched via addition of a proton to form H.sub.2PO.sub.4.sup., is consistent with the mechanism described above.
(53) TABLE-US-00003 TABLE 3 Correlation of Film Absorbance and Thickness with Ion Jones-Dole B Parameters Bilayerd Thickness Ion Name (Formula).sup.a ID.sup.b pK.sub.a.sup.c B.sup.d A(225 nm).sup.e (Std. Dev.).sup.f Chloride (Cl.sup.) CL 0.005.sup.g 0.7558 4.1 (0.1) Fluoride (F.sup.) F 0.107.sup.g 0.7620 3.9 (0.34) Oxalate (C.sub.2O.sub.4.sup.2) OX 4.19 0.164.sup.h 1.4746 9.4 (0.5) Sulfate (SO.sub.4.sup.2) S 0.206.sup.g 1.8176 11.4 (0.5) Acetate (CH.sub.3COO.sup.) AC 4.76 0.227.sup.h 0.7412 3.78 (0.07) Malonate CH.sub.2(COO.sup.).sub.2 MN 4.69 0.285.sup.h 0.8335 4.8 (0.1) Dihydrogenphosphate (H.sub.2PO.sub.4.sup.) DP 7.21 0.340.sup.g 0.7434 4.00 (0.04) Maleate (cis-.sup.OOCCHCHCOO.sup.) ML 6.07 0.348.sup.h 0.9829 6.0 (0.1) Fumarate (trans-.sup.OOCCHCHCOO.sup.) FT 4.44 0.377.sup.h 0.7970 4.3 (0.1) Monohydrogenphosphate (HPO.sub.4.sup.2).sup.i MP 12.32 0.382.sup.g 1.9078 12.0 (0.2) Succinate (.sup.OOCCH.sub.2CH.sub.2COO.sup.) SC 5.61 0.418.sup.h 0.7826 4.0 (0.4) Tartrate (.sup.OOCCH(OH)CH(OH)COO.sup.) TR 4.34 0.469.sup.h 1.0557 5.67 (0.09) .sup.aIon concentrations are 0.05M as Na.sup.+ salts, except where noted, with sufficient added NaCl to give a = 1.00M ionic strength in solution. .sup.bAbbreviation used to identify ions in FIG. 6. .sup.cpK.sub.a (25 C., .fwdarw.0) for the reaction HB.sup. H.sup.+ + B.sup.2 (OX, MN, ML, FT, SC, and TR), for the reaction HB
H.sup.+ + B.sup. (AC), for the reaction H.sub.2B.sup.
H.sup.+ + HB.sup.2 (DP), and for the reaction HB.sup.2
H.sup.+ + B.sup.3 (MP). Values are taken from: CRC Handbook of Chemistry and Physics, 79th Ed.; Lide, D. R., Ed.; CRC Press: New York, 1999; pp. 8-43-8-46. Estimates for corresponding pKa values in the = 1.00M solutions are obtained by subtraction of ~0.9 from each value. .sup.dJones-Dole viscosity B coefficient in water at 25 C. from the indicated reference. .sup.eAbsorbance at 225 nm for a quartz slide coated on both sides with a film of structure Q-EDA/(PSS/PAH).sub.20. .sup.fThickness (and standard deviation) in nm of a PSS/PAH bilayer calculated from AFM measurement of the total thickness of a film deposited on Si wafer having structure Q-EDA/(PSS/PAH).sub.24. .sup.gB value taken from Jenkins et al., Viscosity B-Coefficients of Ions in Solution Chem. Rev. 1995, 95, 2695-2724. .sup.hB value taken from Chmielewska et al., Viscosimetric Studies of Aqueous Solutions of Salts of Carboxylic Acids J. Mol. Liq. 2005, 122, 110-115. .sup.iThe HPO.sub.4.sup.2 ion is present at 0.011M in a solution also containing 0.010M H.sub.2PO.sub.4.sup. and 0.967M NaCl at pH ~6.25 (PSS and rinse solutions) or pH = 6.15 (PAH solution).
Example 5
Analytical Methods
(54) The XPS characterizations of the PEMLs on Si-EDA substrates were performed using the instrument and procedures described in Bassim et al., Layer-by-layer Assembly of Heterogeneous Modular Nanocomposites J. Phys. Chem. C 2012, 116, 1694-1701. FTIR spectra were acquired on a Nicolet FTIR spectrometer coupled to a single-bounce attenuated total reflectance (ATR) accessory (Specac Silvergate) with a 45 Ge prism and 7 mm sample window. Spectra were collected using a MCT detector as 256 scans over the 400-000 cm.sup.1 wavelength region with 4 cm.sup.1 resolution. UV-vis spectra of quartz slides bearing PEML films were acquired using a Cary 5000 UV-vis-NIR double-beam spectrophotometer equipped with Cary WinUV software. A baseline obtained from blank EDA-coated quartz slides was used to correct the UV-vis spectra. Components of the PEMLs produce distinct UV-vis absorbance features: PAH does not absorb above ca. 200 nm, whereas PSS exhibits an absorbance maximum at 225 nm and absorbs strongly below ca. 260 nm. The relative concentrations of available amine and sulfonate sites on terminal PSS and PAH layers were estimated from UV-vis spectra by using, respectively, the Ru(bpy).sub.3(ClO.sub.4).sub.2 and Na.sub.4Ru(BPS).sub.3 complexes (
(55) A NanoSight LM10-HSBF nanoparticle size determination system (Nanosight Inc., Worthington, Ohio, USA) was used for detection and analysis of polyelectrolyte aggregates in 0.04 M Na.sub.2SO.sub.4/0.88 M NaCl and 1.00 M NaCl solutions. The Brownian motion the solutions was tracked and analyzed using the software supplied with the instrument to estimate the hydrodynamic radii and concentrations of any particles present. Because the Nanosight NTA2.1 software was designed to determine the size of hard shell spherical particles, the system cannot provide absolute values for particle size for the polyelectrolyte aggregates having fluxional surface boundaries and shapes. Nevertheless, relative comparisons of the derived parameters for the various polyelectrolytes and solution conditions examined can still be made to provide qualitative information concerning the nature of any aggregates present for samples examined under identical acquisition conditions.
(56) Specifically, the instrument sample solution platen was first rinsed with a salt solution having the same composition as the polyelectrolyte solution to be analyzed and wiped dry with a KimWipe lint-free paper towel. The polyelectrolyte solution containing 2 mg/mL of PAH or PSS in either 0.04 M Na.sub.2SO.sub.4/0.88 M NaCl or 1.00 M NaCl solution was then applied to the platen and particle Brownian motion was recorded at room temperature for 60 s to obtain a video for analysis. Relative brightness (i.e., 0), gain (i.e., 1.00), and blur size (i.e., 5 pixel5 pixel) settings were the same for all samples and the detection threshold was automatically adjusted by the software. Each experiment was repeated 4-8 times to obtain average values and standard deviations for the particle mean diameter, D.sub.50 (i.e., particle median) value, and particle concentration. After removal of the sample solution, the platen was rinsed twice with salt solution having the same composition as the polyelectrolyte solution just analyzed. It was then rinsed successively with water, 70:30 v/v ethanol:water, and water again before being dried with a lint-free paper towel to complete the cleaning process before measurement of the next sample. Control experiments were performed using polyelectrolyte-free 1.00 M NaCl and 0.04 M Na.sub.2SO.sub.4/0.88 M NaCl solutions. Because the results for these controls indicated that the polyelectrolyte-free salt solutions contained essentially no particles (i.e., >100-fold less than analogous solutions containing the polyelectrolytes), no corrections were made to the data obtained for the polyelectrolyte solutions.
(57) Cross-sectional PEML samples for transmission electron microscopy (TEM) were prepared according to the literature procedure (Bassim et al., Layer-by-layer Assembly of Heterogeneous Modular Nanocomposites J. Phys. Chem. C 2012, 116, 1694-1701; Bassim et al. Minimizing Damage during FIB Sample Preparation of Soft Materials J. Microsc. 2012, 245, 288-301) using an FEI Nova Nanolab Focused Ion Beam (FIB) tool operated at 30 kV Ge. The top surface of each PEML was coated with evaporated carbon to minimize ion and electron beam damage during cross-section preparation. The cross-sectional samples were aligned using the [001] direction of the Si substrate and analyzed in scanning transmission electron microscopy high-angle annular darkfield (STEM-HAADF) mode with a 0.5 nm spot size in a JEOL 2200FS microscope operated at 200 keV. The STEM-HAADF image intensity depends on atomic number, Z.sup.1.6, so the contrast in PEMLs was increased by introducing a high-Z stain (Ru(BPS).sub.3.sup.4 dye complex) in one of the polyelectrolyte layers, as described above. Roughness and layer continuity were evaluated by integrated measurements of pixel intensity perpendicular to the layers.
(58) To assess sample surface topography, roughness, and film thickness, AFM experiments were performed in air on a Veeco Nanoman AFM in tapping mode (intermittent contact mode) using TESP tips with 20 nm nominal radii. AFM measurements were made in air to assess sample surface topography, surface roughness, and film thickness. At least 3 images per sample were acquired from regions away from film edges for evaluations of surface topography and roughness. Images for topography evaluation were typically acquired at 1010 m.sup.2 scan sizes, with occasional additional measurements made as necessary at 2020 m.sup.2, 55 m.sup.2, and 22 m.sup.2 scan sizes. Roughness measurements were made in a 25 m.sup.2 region (typically 55 m.sup.2) after processing the image using a flattening routine in the instrument software.
(59) Samples were prepared for AFM measurement of total PEML film thickness by gently scribing PEMLs deposited onto the Si-EDA (or Q-EDA) substrate with stainless steel micro-scope tweezers to create a step edge boundary for the measurement. The scribing was not found to damage the substrate surface. To determine whether any PSS/PAH layers were left after scribing, representative samples were more aggressively scribed using tweezers or a razor blade. AFM measurements indicated removal of a small additional amount of material (ca. 1 nm) consistent with loss of the EDA monolayer from these surfaces, which was observed as a brighter region by optical microscopy (the EDA appearing darker than the native silicon under a microscope objective). While this observation does not prove that there are no PEMLs remaining on the substrate, it is likely that the scribing provides a reasonable and consistent method to assess multilayer thickness.
(60) In some cases the thickness increase noted following deposition of a PAH layer to a PSS-terminated PEML was also measured. Samples for this measurement were prepared by dipping only a portion of the substrate bearing the PSS-terminated PEML into the PAH treatment solution, thereby creating a PSS-PAH step edge boundary to facilitate the measurement. For both the incremental PAH thickness increase and total PEML film thickness, measurements were made by scanning the AFM tip across each step edge boundary at a right angle. A minimum of 18 such measurements were made for each thickness determination, with the average thickness reported. The error for the total film thickness is reported as the standard deviation of the data. The errors reported for the PSS/PAH bilayer thickness and the PAH layer thickness are calculated using the propagation of errors method (chain rule calculus).
Example 6
PAH Aggregation
(61) Evidence of chain-bridging interactions in the =1.00 M solutions (in contrast to previous reports of gelation in low ionic strength solutions (Brunne et al., Biomimetic Synthesis of Silica Nanospheres Depends on the Aggregation and Phase Separation of Polyamines in Aqueous Solution Phys. Chem. Chem. Phys. 2004, 6, 854-857; Murthy et al., Nanoparticle-assembled Capsule Synthesis: Formation of Colloidal Polyamine-Salt Intermediates J. Phys. Chem. B 2006, 110, 25619-25627; Tikhonenko et al., Enzyme-polyelectrolyte Complex: Salt Effects on the Reaction of Urease with Polyallylamine Russ. J. Phys. Chem. A 2009, 83, 1781-1788; Wernersson et al., Effect of Association with Sulfate on the Electrophoretic Mobility of Polyarginine and Polylysine J. Phys. Chem. B 2010, 114, 11934-11941; Dragan et al., Influence of Low-Molecular-Weight Salts on the Formation of Polyelectrolyte Complexes Based on Polycations with Quaternary Ammonium Salt Groups in the Main Chain and Poly(sodium acrylate) Eur. Polym. J. 2001, 37, 1571-1575; Ghimici et al., Behaviour of Cationic Polyelectrolytes upon Binding of Electrolytes: Effects of Polycation Structure, Counterions, and Nature of the Solvent Colloid Polym. Sci. 2002, 280, 130-134; Kova{hacek over (c)}evi et al., The Influence of Ionic strength, Electrolyte Type, and Preparation Procedure on Formation of Weak Polyelectrolyte Complexes Colloids Surf., A 2007, 302, 107-112; Yu et al., Synthesis of Near-Infrared-Absorbing Nanoparticle-Assembled Capsules Chem. Mater. 2007, 19, 1277-1284; Ghimici et al., Interaction of the Low Molecular Weight Salts with Cationic Polyelectrolytes J. Polym. Sci. B: Polym. Phys. 1997, 35, 2571-2581) is obtained by comparing the light scattering data (
(62) TABLE-US-00004 TABLE 4 Light Scattering Analysis of PAH and PSS Salt Solutions Mean Particle Diameter.sup.c D50 Value.sup.d Concentration.sup.e Solution Composition.sup.a No..sup.b (nm) (nm) (particles/mL) PAH (0.04M Na.sub.2SO.sub.4/0.88M NaCl) 8 133 27 116 16 (9.35 0.98) 10.sup.8 PSS (0.04M Na.sub.2SO.sub.4/0.88M NaCl) 7 147 16 138 12 (3.53 1.13) 10.sup.8 PAH (1.00M NaCl) 4 186 10 183 9 (1.50 0.18) 10.sup.8 PSS (1.00M NaCl) 4 144 3 128 17 (6.02 2.45) 10.sup.8 .sup.aIdentity of polyelectrolyte and salt in soution analyzed. Polyelectrolyte is present at 2 mg/mL in each solution. Fresh polyelectrolyte solution was used for each measurement. Sample platen was prepared as described. .sup.bNumber of measurements made. .sup.cEstimated average polyelectrolyte aggregate diameter standard deviation in nm. .sup.dEstimated polyelectrolyte aggregate size standard deviation in nm for which half the aggregates are larger and half are smaller. .sup.eEstimated polyelectrolyte aggregate concentration standard deviation in particles/mL solution.
(63) This behavior of PAH aggregates indicates that SO.sub.4.sup.2 neutralizes PAH chain charge far more effectively than does Cl.sup.. The resulting large numbers of compact PAH aggregates ultimately nucleate gel formation at [SO.sub.4.sup.2]>0.05 M, in agreement with previous observations (Brunner et al., Biomimetic Synthesis of Silica Nanospheres Depends on the Aggregation and Phase Separation of Polyamines in Aqueous Solution Phys. Chem. Chem. Phys. 2004, 6, 854-857; Murthy et al., Nanoparticle-assembled Capsule Synthesis: Formation of Colloidal Polyamine-Salt Intermediates J. Phys. Chem. B 2006, 110, 25619-25627; Tikhonenko et al., Enzyme-polyelectrolyte Complex: Salt Effects on the Reaction of Urease with Polyallylamine Russ. J. Phys. Chem. A 2009, 83, 1781-1788). The neutralization of PAH chain charge by SO.sub.4.sup.2 anions appears to be particularly effective, as the analogous interactions of the PSSSO.sub.3.sup. groups with Na.sup.+ (or Mg.sup.2+) cations do not induce gelation in any of the solutions.
(64) Interactions of PAH solutions with water provide further insight into the properties of PAH aggregates: addition of PAH solutions (=1.00 M) containing SO.sub.4.sup.2 or HPO.sub.4.sup.2 dianions to water resulted in a faint precipitate exhibiting a bluish-white Tyndall effect, which suggests aggregates of 35-40 nm in size. No precipitate was observed upon addition of these PAH solutions to a mixed salt solution (containing the same SO.sub.4.sup.2 or HPO.sub.4.sup.2 dianion), or upon addition of PAH solution containing only univalent salts to water. PAH precipitation in this manner suggests expulsion of excess SO.sub.4.sup.2 during aqueous dilution with concomitant deprotonation of amine sites, resulting in dehydration and collapse of PAH aggregates (
Example 7
PEML Rinsing
(65) To avoid precipitation of PAH (
(66) TABLE-US-00005 TABLE 5 Properties of Multilayer Films Deposited from Salt Solutions (Salt Rinse Protocol) thickness (nm) roughness (nm) salt.sup.a (substrate).sup.b abs..sup.c total film.sup.d bilayer.sup.e r.sub.q.sup.f N.sup.g NaCl.sup.h (Q) 0.7558 NaCl.sup.h (Si) 97.5 2.2 4.1 0.09 0.8 0.4 3 NaF.sup.h (Q) 0.7620 82.5 4.7 4.1 0.2 1.59 0.03 3 NaF.sup.h (Si) 93.9 8.2 3.9 0.3 NaH.sub.2PO.sub.4.sup.h (Q) 0.7434 85.4 3.9 4.3 0.2 0.86 0.06 4 NaH.sub.2PO.sub.4.sup.h (Si) 96.5 1.1 4.02 0.04 0.60 0.03 3 Na(Acetate).sup.h (Q) 0.7412 77.3 0.9 3.78 0.04 0.71 0.02 3 Na(Acetate).sup.h (Si) 90.8 1.7 3.78 0.04 0.9 0.4 3 MgCl.sub.2.sup.i (Q) 0.7358 88.6 1.8 4.43 0.09 2.3 0.5 2 MgCl.sub.2.sup.i (Si) 93.2 1.4 3.88 0.06 1.0 0.5 4 MgSO.sub.4.sup.j (Q) 1.5001 MgSO.sub.4.sup.j (Si) 228.6 6.3 9.5 0.3 1.2 0.4 3 MgSO.sub.4.sup.i (Q) 1.8176 226.8 3.7 11.3 0.2 1.1 0.1 2 MgSO.sub.4.sup.i (Si) 274 13 11.4 0.5 Na.sub.2HPO.sub.4.sup.k (Q) 1.9078 Na.sub.2HPO.sub.4.sup.k (Si) 286.9 3.7 12.0 0.2 1.4 0.4 3 Na.sub.2Oxalate.sup.i (Q) 1.4746 192.8 5.2 9.6 0.3 1.93 0.05 5 Na.sub.2Oxalate.sup.i (Si) 227.0 12.0 9.4 0.5 0.9 0.2 3 Na.sub.2Tartrate.sup.i (Q) 1.0557 119.0 2.2 6.0 0.1 1.34 0.39 3 Na.sub.2Tartrate.sup.i (Si) 139.0 2.3 5.67 0.09 0.78 0.04 3 Na.sub.2Maleate.sup.i (Q) 0.9829 Na.sub.2Maleate.sup.i (Si) 144.8 3.1 6.0 0.1 1.10 0.18 3 Na.sub.2Fumarate.sup.i (Q) 0.7970 Na.sub.2Fumarate.sup.i (Si) 104.1 2.6 4.3 0.1 1.54 0.97 3 Na.sub.2Malonate.sup.i (Q) 0.8335 Na.sub.2Malonate.sup.i (Si) 114.6 2.6 4.8 0.1 0.92 0.10 3 Na.sub.2Succinnnate.sup.i (Q) 0.7826 Na.sub.2Succinnnate.sup.i (Si) 97.1 9.0 4.00 0.37 0.94 0.07 3 .sup.aIdentity of the univalent or divalent ion salt present in each polyelectrolyte solution (and initial rinse solution) during multilayer deposition. The listed salts are present at 0.05M concentration, unless otherwise noted, with sufficient NaCl added to fix the solution ionic strength at = 1.00M. .sup.bIdentity of the substrate coated with the multilayer. Q = quartz and Si = silicon wafer. Substrates were coated with a chemisorbed EDA organosiloxane monolayer prior to multilayer deposition. Multilayer films have structure: Substrate-EDA/(PSS/PAH).sub.x (x = 20 for quartz (Q) and x = 24 for silicon (Si) substrates). .sup.cUV absorbance of the multilayer film at 225 nm on a quartz substrate. .sup.dThickness (std. dev.) of the multilayer film as determined by AFM. .sup.eAverage thickness (std. dev.) of a single PSS/PAH bilayer within the multilayer film, calculated by division of the total film thickness by the number of bilayers deposited, x, with x = 20 (quartz) or x = 24 (Si). .sup.fRoot-mean-square roughness, r.sub.q, (std. dev.) from AFM measurements. .sup.gNumber of measurements taken to determine roughness values. .sup.hSolution also contains 0.95M NaCl for ionic strength adjustment. .sup.iSolution also contains 0.85M NaCl for ionic strength adjustment. .sup.jSolution also contains 0.80M NaCl for ionic strength adjustment. .sup.kPAH solubility limits the Na.sub.2HPO.sub.4 concentration to 0.011M, with 0.957M NaCl, and with 0.010M NaH.sub.2PO.sub.4 added for ionic strength and pH buffer control.
Example 8
Effects of Anions on PEML Properties
(67) Among the PEMLs prepared (via the salt rinse protocol) in the presence of various ions listed in
Example 9
Film Compositions
(68) To interpret the observations summarized in Table 5, the composition of the PEMLs was examined, with particular emphasis on films prepared in the presence of SO.sub.4.sup.2 (
(69) The presence of 0.05 M univalent anions vs divalent SO.sub.4.sup.2 anions produces the most dramatic effect on the film composition. In the presence of univalent anions, PAH and PSS adsorb in a nearly 1:1 ratio and few Cl.sup. ions are required to neutralize the excess PAH component. In contrast, ca. 2-3 times more PAH than PSS is deposited from solutions containing SO.sub.4.sup.2. Therefore, those films contain large amounts of Cl.sup. ions to charge-compensate the terminal PAH layers.
(70)
(71) The release of Ru(BPS).sub.3.sup.4 dye from the PAH during PSS treatment indicates that separate populations of strongly and weakly bound dye exist in the terminal PAH layer of the growing PSS/PAH/Ru film. Therefore, at least two separate environments, presumably corresponding to extended and coiled PAH chains (Feldt et al., Influence of Salt and Rinsing Protocol on the Structure of PAH/PSS Polyelectrolyte MultilayersLangmuir 2010, 26, 17048-17057), are present in the terminal PAH layer during dye deposition.
Example 10
Sulfate Solution Concentration
(72) The unusual behavior of PEMLs prepared from solutions containing SO.sub.4.sup.2, as summarized in
(73) TABLE-US-00006 TABLE 6 Dependence of Multilayer Films Properties on Solution Sulfate Concentration Associated with FIG. 4A (Salt Rinse Protocol) salt.sup.a concentration.sup.c thickness (nm) roughness (nm) (substrate).sup.b Na.sub.2SO.sub.4 NaCl abs..sup.d total film.sup.e bilayer.sup.f r.sub.q.sup.g N.sup.h NaCl (Q) 0.00 1.00 0.7558 NaCl (Si) 0.00 1.00 97.5 2.2 4.10 0.1 0.8 0.4 3 Na2SO4 (Q) 0.05 0.85 1.8176 226.8 3.7 11.3 0.2 1.14 0.1 3 Na2SO4 (Si) 0.05 0.85 274.2 12.8 11.4 0.5 Na2SO4 (Q) 0.043 0.871 1.2806 Na2SO4 (Si) 0.043 0.871 175.1 0.2 7.3 0.1 0.64 0.03 3 Na2SO4 (Q) 0.04 0.88 1.2425 Na2SO4 (Si) 0.04 0.88 151.5 1.9 7.6 0.1 1.22 0.09 3 Na2SO4 (Q) 0.037 0.889 1.1105 Na2SO4 (Si) 0.037 0.889 148.0 2.3 6.2 0.1 1.5 1.0 5 Na2SO4 (Q) 0.03 0.91 0.9785 Na2SO4 (Si) 0.03 0.91 134.6 3.0 5.6 0.1 0.8 0.3 3 Na2SO4 (Q) 0.01 0.97 0.8061 Na2SO4 (Si) 0.01 0.97 103.5 1.1 4.3 0.1 0.9 0.1 3 .sup.aIdentity of the salt present in each polyelectrolyte solution (and initial rinse solution for sulfate-containing solutions) during multilayer deposition. .sup.bIdentity of the substrate coated with the multilayer. Q = quartz and Si = silicon wafer. Substrates were coated with a chemisorbed EDA organosiloxane monolayer prior to multilayer deposition. Multilayer films have structure: Substrate-EDA/(PSS/PAH).sub.x (x = 20 for quartz (Q) and x = 24 for silicon (Si) substrates). .sup.cLeft column indicates the molar concentration of Na.sub.2SO.sub.4 in the polyelectrolyte treatment solution and first rinse solutions. Right column indicates the concentration of NaCl present to bring the solution ionic strength to = 1.00M. .sup.dUV absorbance of the multilayer film at 225 nm on a quartz substrate. .sup.eThickness std. dev. of the multilayer film as determined by AFM. .sup.fAverage thickness (std. dev.) of a single PSS/PAH bilayer within the multilayer film, calculated by division of the total film thickness by the number of bilayers deposited, x, with x = 20 (quartz) or x = 24 (Si). .sup.gRoot-mean-square roughness, r.sub.q, (std. dev.) from AFM measurements. .sup.hNumber of measurements taken to determine roughness values.
(74) The increase in PEML absorbance due to the presence of SO.sub.4.sup.2, A, can be estimated for each SO.sub.4.sup.2 solution in
(75) The Ru(bpy).sub.3(ClO.sub.4).sub.2 and Na.sub.4Ru(BPS).sub.3 complexes were also used to estimate the relative concentrations of available amine and sulfonate sites present on terminal PSS and PAH polyelectrolyte layers, respectively, for PSS/PAH multilayers deposited from 0.037 M Na.sub.2SO.sub.4/0.889 M NaCl solutions. A Q-EDA/(PSS/PAH).sub.2/PSS film was treated 4 h with aqueous 4.310.sup.4 M Ru(bpy).sub.3(ClO.sub.4).sub.2 solution, followed by a triple water rinse, to electrostatically bind Ru(bpy).sub.3.sup.2+ cation to available SO.sub.3.sup. groups present in the terminal PSS layer of the film. Analogous treatment of a Q-EDA/(PSS/PAH).sub.2 film with aqueous 4.010.sup.4 M Na.sub.4Ru(BPS).sub.3 solution bound Ru(BPS).sub.3.sup.4 anion to available NH.sub.3+ sites on the terminal PAH layer in this film. Binding occurs without decomposition of the dyes, as shown by maintenance of dye fluorescence in the films (note
[Ru]=A/(2)(3)
Taking a ratio of [Ru] terms from Eq. (3) for the bound Ru(BPS).sub.3.sup.4 and Ru(bpy).sub.3.sup.2+ species and recognizing that each Ru(bpy).sub.3.sup.2+ cation could bind at most two SO.sub.3.sup. groups and each Ru(BPS).sub.3.sup.4 anion could bind at most six NH.sub.3.sup.+ groups leads, after some simplification, to Eq. (4) as an estimate of the ratio of available amine sites at a terminal PAH layer to available sulfonate sites on a terminal PSS layer, [NH.sub.3.sup.+]/[SO.sub.3.sup.]:
[NH.sub.3.sup.+]/[SO.sub.3.sup.]A/(3A)(4)
In Eq. (4), A and are values associated with bound Ru(BPS).sub.3.sup.4 anion and A and are values associated with bound Ru(bpy).sub.3.sup.2+ cation. The =2.510.sup.7 cm.sup.2.Math.mole.sup.1) and =1.410.sup.7 cm.sup.2.Math.mole.sup.1 values for the metal-to-ligand charge-transfer (i.e., MLCT) absorbance band at 460 nm appropriate for complexes adsorbed to the polyelectrolyte surfaces were calculated from corresponding (L.Math.mole.sup.1.Math.cm.sup.1) values measured from UV-visible absorption maxima for aqueous solutions containing known concentrations of each complex using Eq. (5):
(cm.sup.2.Math.mole.sup.1)=(L.Math.mole.sup.1.Math.cm.sup.1)1000 cm.sup.3.Math.L.sup.1(5)
Use of Eq. (4) is strictly valid only if binding of the complex to the polyelectrolyte layer does not affect the oscillator strength of the electronic transition corresponding to the absorbance band. In fact, small shifts in the positions and bandwidths of the absorbance peak maxima are observed for both complexes in the experiment after binding to the polyelectrolyte layer (vide infra), suggesting that some small change in likely occurs. Therefore, absorbance bands of the same parentage are selected in each complex (i.e., MLCT d-*) for the calculation in Eq. (4) in order to minimize the effect of perturbations.
Example 11
Linear Film Growth
(76) In
(77) PEML growth from 0.04 M Na.sub.2SO.sub.4/0.88 M NaCl solutions was examined for any signs of exponential behavior by monitoring the absorbance, thickness, and roughness as a function of the number of PSS/PAH bilayers (Table 7). The average PSS/PAH bilayer thickness increases from <7 nm to 7.1-7.6 nm for films containing >8 PSS/PAH bilayers, consistent with previous observations (Soltwedel et al., Interdiffusion in Polyelectrolyte Multilayers Macromolecules 2010, 43, 7288-7293) for PSS/PAH deposition from 1.00 M NaCl (aq) solutions. In addition, no correlation is observed between film roughness and the number of deposited PSS or PAH layers. These observations are indicative of linear, rather than exponential, PEML growth for films deposited from solutions having fixed [SO.sub.4.sup.2]. Linear film growth consistent with previous observations (Soltwedel et al., Interdiffusion in Polyelectrolyte Multilayers Macromolecules 2010, 43, 7288-7293; Kiel et al. Structural Characterization of a Spin-assisted Colloid-polyelectrolyte Assembly: Stratified Multilayer Thin Films Langmuir 2010, 26, 18499-18502; Korneev et al., Neutron Reflectivity Analysis of Self-assembled Film Superlattices with Alternate Layers of Deuterated and Hydrogenated Polysterenesulfonate and Polyallylamine Physica B 1995, 213-214, 954-956) is confirmed for the PEMLs by linear correlations of both the film absorbance and total thickness with the number of deposited PSS/PAH bilayers (
(78) TABLE-US-00007 TABLE 7 PEML Properties as Functions of the Number of PSS/PAH Bilayers (Salt Rinse Protocol) thickness (nm) no..sup.a substrate.sup.b/[film structure].sup.c abs..sup.d total film.sup.e bilayer.sup.f roughness (nm) r.sub.q.sup.g 3.5 Si/[(PSS/PAH).sub.3/PSS] 21.8 6.2 6.2 0.3 1.85 0.2 4 Q/[(PSS/PAH).sub.4] 0.2371 4 Si/[(PSS/PAH).sub.4] 20.3 1.8 5.1 0.5 1.4 0.2 7.5 Si/[(PSS/PAH).sub.7/PSS] 50.7 1.7 6.7 0.2 1.7 0.1 8 Q/[(PSS/PAH).sub.8] 0.4296 8 Si/[(PSS/PAH).sub.8] 53.3 1.1 6.7 0.1 1.48 0.02 8.5 Q/[(PSS/PAH).sub.8/PSS] 0.5046 8.5 Si/[(PSS/PAH).sub.8/PSS] 60.1 1.3 7.1 0.2 1.46 0.14 9 Q/[(PSS/PAH).sub.9] 0.4978 11.5 Si/[(PSS/PAH).sub.11/PSS] 84.9 2.4 7.4 0.2 1.31 0.13 12 Q/[(PSS/PAH).sub.12] 0.7426 12 Si/[(PSS/PAH).sub.12] 87.1 1.7 7.3 0.1 0.89 0.13 13 Q/[(PSS/PAH).sub.13] 0.8028 15.5 Si/[(PSS/PAH).sub.15/PSS] 113.6 1.3 7.33 0.08 1.4 0.2 16 Q/[(PSS/PAH).sub.16] 0.9611 16 Si/[(PSS/PAH).sub.16] 117.6 1.3 7.35 0.06 0.99 0.08 19.5 Si/[(PSS/PAH).sub.19/PSS] 147.6 2.3 7.6 0.1 1.34 0.34 20 Q/[(PSS/PAH).sub.20] 1.2425 20 Si/[(PSS/PAH).sub.20] 151.5 1.9 1.9 1.22 0.09 .sup.aNumber of PSS/PAH bilayers deposited from 0.04M Na.sub.2SO.sub.4/0.88M NaCl (aq) solution. .sup.bIdentity of the substrate coated with the multilayer. Q = quartz and Si = silicon wafer. Substrates were coated with a chemisorbed EDA organosiloxane monolayer prior to multilayer deposition. Multilayer films have structure: Substrate-EDA/(PSS/PAH).sub.x (x = 20 for quartz (Q) and x = 24 for silicon (Si) substrates). .sup.cPSS and PAH layer structure of the multilayer film identified by the bilayer code in column 1. The EDA monolayer on the Q and Si substrates is omitted for clarity. .sup.dUV absorbance of the multilayer film at 225 nm on a quartz substrate. .sup.eThickness (std. dev.) of the multilayer film as determined by AFM. .sup.fAverage thickness (std. dev.) of a single PSS/PAH bilayer within the multilayer film, calculated by dividing the total film thickness (column 4) by the number of bilayers deposited (column 1). .sup.gRoot-mean-square roughness, r.sub.q, (std. dev.) from AFM measurements at three film surface sites.
Example 12
TEM Strata Imaging
(79) To observe the linear growth regime by direct TEM imaging, PEMLs with PAH layers stained by Ru(BPS).sub.3.sup.4 shown in
(80) TABLE-US-00008 TABLE 8 Absorbance Data for PAH/PSS/Ru(bathophenanthrolinesulfonate).sub.3.sup.4 Trilayer-based Multilayer Films Associated with FIGS. 5B and C Abs..sup.c Abs..sup.c Abs..sup.c Row Trilayer No..sup.a Film Structure.sup.b (225 nm) (286 nm) (464 nm) 1.sup.d,e 3 [(PSS/PAH/Ru.sup.f).sub.3/PSS/PAH] 0.37026 0.303833 0.093956 2.sup.e,g 4 [(PSS/PAH/Ru).sub.4] 0.494527 0.461827 0.147875 3.sup.g,h 4 [(PSS/PAH/Ru).sub.4/PSS] 0.468506 0.392885 0.127366 4.sup.d,h 4 [(PSS/PAH/Ru).sub.4/PSS/PAH] 0.454794 0.385262 0.127633 5.sup.d 7 [(PSS/PAH/Ru).sub.7/PSS/PAH] 0.792142 0.597925 0.20253 6.sup.d 8 [(PSS/PAH/Ru).sub.8/PSS/PAH] 0.933085 0.704562 0.224804 7.sup.d 9 [(PSS/PAH/Ru).sub.9/PSS/PAH] 1.0654 0.818163 0.242362 8.sup.h 10 [(PSS/PAH/Ru).sub.10/PSS] 1.185596 0.917511 0.259185 9.sup.d,e,h 10 [(PSS/PAH/Ru).sub.10/PSS/PAH] 1.176532 0.917933 0.254785 10.sup.e,g 11 [(PSS/PAH/Ru).sub.11] 1.320172 1.101638 0.291548 11.sup.g,h 11 [(PSS/PAH/Ru).sub.11/PSS] 1.29084 1.028493 0.273427 12.sup.d,h 11 [(PSS/PAH/Ru).sub.11/PSS/PAH] 1.284034 1.021332 0.271213 13.sup.d 12 [(PSS/PAH/Ru).sub.12/PSS/PAH] 1.399545 1.117805 0.293125 14.sup.d 13 [(PSS/PAH/Ru).sub.13/PSS/PAH] 1.526528 1.22435 0.314943 15.sup.d 16 [(PSS/PAH/Ru).sub.16/PSS/PAH] 1.910164 1.481669 0.397298 16.sup.d 16 [(PSS/PAH/Ru).sub.16/PSS/PAH] 1.907305 1.478812 0.398347 17.sup.d 16 [(PSS/PAH/Ru).sub.16/PSS/PAH] 1.908482 1.479037 0.403783 18.sup.d 18 [(PSS/PAH/Ru).sub.18/PSS/PAH] 2.14154 1.679246 0.474377 19.sup.d,e 19 [(PSS/PAH/Ru).sub.19/PSS/PAH] 2.266959 1.79541 0.509651 20.sup.e 20 [(PSS/PAH/Ru).sub.20] 2.434432 1.978641 0.565566 .sup.aNumber of PAH/PSS/Ru(bathophenanthrolinesulfonate).sub.3.sup.4 trilayers present in the film. .sup.bInternal structure of the multilayer film on the EDA-coated quartz slide (not shown). Polyelectrolyte layers were deposited from separate solutions containing 2 mg PSS or PAH/mL of 0.04M Na.sub.2SO.sub.4/0.85M NaCl solution. Polyelectrolyte layers were sequentially rinsed using 0.04M Na.sub.2SO.sub.4/0.85M NaCl solution, 1.00M NaCl solution, and water according to the salt rinse protocol. The Ru deposition solution comprised 2 mg Na.sub.4Ru(bathophenanthrolinesulfonate).sub.3/mL water. The Ru layers were triple rinsed in water. Films were dried in a filtered N.sub.2 gas stream after deposition of each layer. .sup.cUV-visible film absorbance of the indicated wavelength. Uncertainty is 0.005 abs. units. .sup.dData for films in these rows are used to prepare the plot shown in FIG. 5C. .sup.eComparison of data in adjacent rows marked e indicates the effect on absorbance of the addition of a Ru layer to a film terminated by a PAH layer, e.g., rows 1 vs. 2, 9 vs. 10. .sup.fRu represents the Ru(bathophenanthrolinesulfonate).sub.3.sup.4 transition metal complex ion, where bathophenanthrolinesulfonate is 4,7-di-(m-sulfonatophenyl)-1,10-phenanthroline, in each film structure. .sup.gComparison of data in adjacent rows marked g indicates the effect on film absorbance of the addition of a PSS layer to the multilayer film terminated by a Ru layer, e.g., rows 2 vs. 3. .sup.hComparison of data in adjacent rows marked h indicates the effect on film absorbance of the addition of a PAH layer to the multilayer film terminated by a PSS layer, e.g., rows 3 vs. 4.
(81) Despite the partial displacement of Ru(BPS).sub.3.sup.4 dye during PSS deposition, the analysis of absorbance data at 225 nm, 286 nm, and 460 nm in
(82) The STEM-HAADF image of the cross section of a Si-EDA/(PSS/PAH/Ru).sub.20 PEML is shown in
Example 13
Surface Morphology
(83) Examination of the topography of the PEMLs described in Table 7 and
(84) Specifically, large defects with sizes of ca. 200-400 nm and depths of ca. 4-8 nm are observed for films having 4 PSS/PAH bilayers. After deposition of ca. 8 PSS/PAH bilayers, defects become shallower, with depths of ca. 3-6 nm. For films with 12 PSS/PAH bilayers, defect diameters decrease to ca. 100-250 nm and depths to ca. 1-2 nm. These morphological changes suggest a transition from initial electrostatic adsorption of intact polyelectrolyte solution aggregates (
(85) It is noted that the TEM and AFM data in
Example 14
Testing the Anion Bridging Model
(86) The model summarized in
(87)
(88)
(89)
Example 15
Anion Structure Effects
(90) In the data summarized in
(91) The series of dicarboxylates analyzed in
(92) The effect of changing the rigidity is most pronounced in comparing maleate to succinate (SC), in which CO.sup. sites are connected much less rigidly by a pair of methylene groups. As a result, succinate produces the lowest PEML thickness among the dicarboxylates in
(93) The apparently weak bridging activity of succinate also suggests introduction of functional groups that are capable of supplementing electrostatic bridging by additional noncovalent interactions within the PAH aggregate. The carboxylate groups of tartrate (TR) are separated by a flexible two-carbon chain analogous to succinate. However, the presence of a single hydroxyl group on each of the carbon atoms in the chain dramatically increases film absorbance and thickness to levels rivaling maleate, i.e., overcoming the penalty to the bridging activity due to chain flexibility.
(94) The behavior of PEMLs fabricated using the dicarboxylate species clearly indicates that factors such as size and rigidity of the bridging anion, together with the angle presented by its negatively charged bridging sites, are important in controlling the PAH aggregate properties required for PEML fabrication using the methods. Smaller anions having (at least) two closely spaced bridging sites that are rigidly fixed at an obtuse angle are best structured to provide optimal bridging behavior and PEML deposition following the model in
(95) Obviously, many modifications and variations are possible in light of the above teachings. It is therefore to be understood that the claimed subject matter may be practiced otherwise than as specifically described. Any reference to claim elements in the singular, e.g., using the articles a, an, the, or said is not construed as limiting the element to the singular.