Fungicidal composition having synergistic effect

Abstract

A fungicidal composition having a synergistic effect is provided. The composition includes active ingredients A and B. The active ingredient A is polyhexamethylene biguanide or an agriculturally acceptable salt thereof, the active ingredient B is one selected from cyprodinil, epoxiconazole, benthiavalicarb-isopropyl, zoxamide, azoxystrobin, prothioconazole, difenoconazole, fenamidone, polyoxin, iprodione, acibenzolar, dithianon, pyraclostrobin, trifloxystrobin, picoxystrobin, fluazinam, thifluzamide or dimethomorph, and the weight ratio of the two ingredients is from 1:50 to 50:1. The test results show that the fungicidal composition according to the present invention has an obvious synergistic effect, and importantly, the application rate is reduced, such that the cost is lowered. The application rate of a single agent alone is effectively reduced by combining the fungicides of different mechanisms and modes of action, which is beneficial for broadening the fungicidal spectrum, retarding the resistance development of the fungi and improving the control effect.

Claims

1. A fungicidal composition having a synergistic effect, comprising active ingredients A and B, wherein the active ingredient A is polyhexamethylene biguanide or an agriculturally acceptable salt thereof, the active ingredient B is one selected from cyprodinil, epoxiconazole, benthiavalicarb-isopropyl, zoxamide, azoxystrobin, prothioconazole, difenoconazole , fenamidone, polyoxin, iprodione, acibenzolar, dithianon, pyraclostrobin, trifloxystrobin, picoxystrobin, fluazinam, thifluzamide, and dimethomorph, and a weight ratio of the active ingredient A to the active ingredient B is from 1:10 to 10:1.

2. The fungicidal composition according to claim 1, wherein the agriculturally acceptable salt of polyhexamethylene biguanide is one of polyhexamethylene biguanide hydrochloride, polyhexamethylene biguanide nitrate, polyhexamethylene biguanide carbonate, polyhexamethylene biguanide phosphate, polyhexamethylene biguanide sulfate, polyhexamethylene biguanide stearate, and polyhexamethylene biguanide acetate.

3. The fungicidal composition according to claim 1, wherein the agriculturally acceptable salt of polyhexamethylene biguanide is polyhexamethylene biguanide hydrochloride.

4. The fungicidal composition according to claim 1, comprising 6-92% by weight of the active ingredients and further comprising 94-8% by weight of pesticide adjuvants.

5. The fungicidal composition according to claim 4, which is prepared into pesticidally acceptable formations with the active ingredients and the pesticide adjuvants.

6. The fungicidal composition according to claim 5, which is in the form of a powder or a wettable powder prepared with the active ingredients and the pesticide adjuvants.

7. The fungicidal composition according to claim 1 is implemented to control at least one disease on crops in the agricultural area.

8. The fungicidal composition according to claim 1 is implemented to control at least one of cucumber downy mildew or wheat rust.

Description

DETAILED DESCRIPTION

(1) To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is described in further detail with reference to examples. It should be understood that specific examples described herein are merely provided for explaining, instead of limiting the present invention. Any modifications, equivalent replacements, and improvements made without departing from the spirit and principle of the present invention fall within the protection scope of the present invention.

(2) The percentages given in all the formulations in the examples below are all weight percentages. The various formulations are processed from the composition of the present invention by a process known in the prior art which may be varied as desired.

(3) I. Preparation Example Of Formulations

(4) (I) Processing of Wettable Powder and Examples

(5) The active ingredients polyhexamethylene biguanide or a salt thereof and one of cyprodinil, epoxiconazole, benthiavalicarb-isopropyl, zoxamide, azoxystrobin, prothioconazole, difenoconazole, fenamidone, polyoxin, iprodione, acibenzolar, dithianon, pyraclostrobin, trifloxystrobin, picoxystrobin, fluazinam, thifluzamide, and dimethomorph were fully mixed with various adjuvants and fillers in proportion, and ground by an ultra-fine grinder, to obtain a wettable powder.

Example 1

62% Polyhexamethylene Biguanide Hydrochloride.Cyprodinil Wettable Powder

(6) polyhexamethylene biguanide hydrochloride 60%, cyprodinil 2%, a sodium alkyl naphthalene sulfonate 4%, sodium dodecyl sulfonate 3%, ammonium sulfate 3%, and light calcium carbonate q.s. to 100%.

Example 2

50% Polyhexamethylene Biguanide Hydrochloride.Cyprodinil Wettable Powder

(7) polyhexamethylene biguanide hydrochloride 25%, cyprodinil 25%, sodium lignin sulfonate 6%, sodium dodecyl sulfonate 3%, xanthan gum 1%, sodium carboxymethyl starch 1%, and attapulgite clay q.s. to 100%.

Example 3

65% Polyhexamethylene Biguanide Hydrochloride.Cyprodinil Wettable Powder

(8) polyhexamethylene biguanide hydrochloride 2%, cyprodinil 63%, sodium lignin sulfonate 5%, a sodium methylnaphthalene sulfonate formaldehyde condensate 7%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 4

85% Polyhexamethylene Biguanide Hydrochloride.Epoxiconazole Wettable Powder

(9) polyhexamethylene biguanide hydrochloride 83%, epoxiconazole 2%, ammonium sulfate 1%, sodium alginate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%.

Example 5

30% Polyhexamethylene Biguanide Acetate.Epoxiconazole Wettable Powder

(10) polyhexamethylene biguanide acetate 15%, epoxiconazole 15%, sodium dodecyl sulfonate 2%, a sodium alkyl naphthalene sulfonate 2%, ammonium sulfate 3%, and light calcium carbonate q.s. to 100%.

Example 6

75% Polyhexamethylene Biguanide Carbonate.Epoxiconazole Wettable Powder

(11) polyhexamethylene biguanide carbonate 2%, epoxiconazole 73%, a sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignin sulfonate 4%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 7

62% Polyhexamethylene Biguanide Hydrochloride Benthiavalicarb-Isopropyl Wettable Powder

(12) polyhexamethylene biguanide hydrochloride 60%, benthiavalicarb-isopropyl 2%, sodium carboxymethyl starch 1%, sodium dodecyl sulfonate 4%, sodium lignin sulfonate 4%, xanthan gum 1%, and attapulgite clay q.s. to 100%.

Example 8

40% Polyhexamethylene Biguanide Stearate.Benthiavalicarb-Isopropyl Wettable Powder

(13) polyhexamethylene biguanide stearate 20%, benthiavalicarb-isopropyl 20%, ammonium sulfate 1%, sodium alginate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%.

Example 9

75% Polyhexamethylene Biguanide.Benthiavalicarb-Isopropyl Wettable Powder

(14) polyhexamethylene biguanide 2%, benthiavalicarb-isopropyl 73%, a sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignin sulfonate 4%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 10

85% Polyhexamethylene Biguanide Hydrochloride.Zoxamide Wettable Powder

(15) polyhexamethylene biguanide hydrochloride 82%, zoxamide 3%, calcium lignin sulfonate 2%, sodium dodecylbenzene sulfonate 1%, bentonite 2%, and attapulgite clay q.s. to 100%.

Example 11

60% Polyhexamethylene Biguanide.Zoxamide Wettable Powder

(16) polyhexamethylene biguanide 30%, zoxamide 30%, an alkylpolyoxyethylene ether sulfonate 1%, nekal 2%, bentonite 1.5%, white carbon black 2%, and diatomaceous earth q.s. to 100%.

Example 12

75% Polyhexamethylene Biguanide Sulfate.Zoxamide Wettable Powder

(17) polyhexamethylene biguanide sulfate 2%, zoxamide 73%, an alkylsulfonate 6%, sodium lignin sulfonate 6%, white carbon black 5%, and kaolin q.s. to 100%.

Example 13

88% Polyhexamethylene Biguanide Hydrochloride.Azoxystrobin Wettable Powder

(18) polyhexamethylene biguanide hydrochloride 86%, azoxystrobin 2%, a polyoxyethylene octyl phenyl ether 2%, sodium lignin sulfonate 6%, white carbon black 4%, and diatomaceous earth q.s. to 100%.

Example 14

50% Polyhexamethylene Biguanide.Azoxystrobin Wettable Powder

(19) polyhexamethylene biguanide 25%, azoxystrobin 25%, calcium lignin sulfonate 7%, white carbon black 5%, sodium dodecylbenzene sulfonate 3%, and attapulgite clay q.s. to 100%.

Example 15

62% Polyhexamethylene Biguanide Acetate.Azoxystrobin Wettable Powder

(20) polyhexamethylene biguanide acetate 2%, azoxystrobin 60%, calcium lignin sulfonate 5%, bentonite 4%, a polyoxyethylene octyl phenyl ether 3%, and attapulgite clay q.s. to 100%.

Example 16

85% Polyhexamethylene Biguanide Hydrochloride.Prothioconazole Wettable Powder

(21) polyhexamethylene biguanide hydrochloride 83%, prothioconazole 2%, a polyoxyethylene octyl phenyl ether 1%, sodium lignin sulfonate 2%, white carbon black 3%, and diatomaceous earth q.s. to 100%.

Example 17

50% Polyhexamethylene Biguanide.Prothioconazole Wettable Powder

(22) polyhexamethylene biguanide 25%, prothioconazole 25%, sodium dodecylbenzene sulfonate 3%, white carbon black 5%, calcium lignin sulfonate 7%, and attapulgite clay q.s. to 100%.

Example 18

85% Polyhexamethylene Biguanide Stearate.Prothioconazole Wettable Powder

(23) polyhexamethylene biguanide stearate 3%, prothioconazole 82%, calcium lignin sulfonate 5%, bentonite 4%, a polyoxyethylene octyl phenyl ether 3%, and attapulgite clay q.s. to 100%.

Example 19

88% Polyhexamethylene Biguanide.Difenoconazole Wettable Powder

(24) polyhexamethylene biguanide 86%, difenoconazole 2%, sodium dodecylbenzene sulfonate 2%, bentonite 1%, calcium lignin sulfonate 2%, and attapulgite clay q.s. to 100%.

Example 20

50% Polyhexamethylene Biguanide Carbonate.Difenoconazole Wettable Powder

(25) polyhexamethylene biguanide carbonate 25%, difenoconazole 25%, sodium lignin sulfonate 6%, an alkylsulfonate 6%, white carbon black 11%, and kaolin q.s. to 100%.

Example 21

86% Polyhexamethylene Biguanide Hydrochloride.Difenoconazole Wettable Powder

(26) polyhexamethylene biguanide hydrochloride 2%, difenoconazole 84%, nekal 1%, an alkylpolyoxyethylene ether sulfonate 2%, bentonite 1.5%, white carbon black 2%, and diatomaceous earth q.s. to 100%.

Example 22

62% Polyhexamethylene Biguanide Hydrochloride.Fenamidone Wettable Powder

(27) polyhexamethylene biguanide hydrochloride 60%, fenamidone 2%, a sodium alkyl naphthalene sulfonate 4%, sodium dodecyl sulfonate 3%, ammonium sulfate 3%, and light calcium carbonate q.s. to 100%.

Example 23

50% Polyhexamethylene Biguanide Acetate.Fenamidone Wettable Powder

(28) polyhexamethylene biguanide hydrochloride 25%, fenamidone 25%, sodium lignin sulfonate 6%, sodium dodecyl sulfonate 3%, xanthan gum 1%, sodium carboxymethyl starch 1%, and attapulgite clay q.s. to 100%.

Example 24

65% Polyhexamethylene Biguanide Hydrochloride.Fenamidone Wettable Powder

(29) polyhexamethylene biguanide hydrochloride 2%, fenamidone 63%, sodium lignin sulfonate 5%, a sodium methylnaphthalene sulfonate formaldehyde condensate 7%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 25

85% Polyhexamethylene Biguanide Hydrochloride.Polyoxin Wettable Powder

(30) polyhexamethylene biguanide hydrochloride 83%, polyoxin 2%, sodium alginate 3%, ammonium sulfate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%.

Example 26

30% Polyhexamethylene Biguanide Acetate.Polyoxin Wettable Powder

(31) polyhexamethylene biguanide acetate 15%, polyoxin 15%, sodium dodecyl sulfonate 2%, a sodium alkyl naphthalene sulfonate 2%, ammonium sulfate 3%, and light calcium carbonate q.s. to 100%.

Example 27

75% Polyhexamethylene Biguanide Carbonate.Polyoxin Wettable Powder

(32) polyhexamethylene biguanide carbonate 2%, polyoxin 73%, a sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignin sulfonate 4%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 28

62% Polyhexamethylene Biguanide Hydrochloride.Iprodione Wettable powder

(33) polyhexamethylene biguanide hydrochloride 60%, iprodione 2%, ammonium sulfate 3%, sodium carboxymethyl starch 1%, sodium dodecyl sulfonate 4%, sodium lignin sulfonate 4%, xanthan gum 1%, and attapulgite clay q.s. to 100%.

Example 29

40% Polyhexamethylene Biguanide Stearate.Iprodione Wettable Powder

(34) polyhexamethylene biguanide stearate 20%, iprodione 20%, ammonium sulfate 1%, sodium alginate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%.

Example 30

75% Polyhexamethylene Biguanide.Iprodione Wettable Powder

(35) polyhexamethylene biguanide 2%, iprodione 73%, a sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignin sulfonate 4%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 31

85% Polyhexamethylene Biguanide Hydrochloride.Acibenzolar Wettable Powder

(36) polyhexamethylene biguanide hydrochloride 82%, acibenzolar 3%, calcium lignin sulfonate 2%, sodium dodecylbenzene sulfonate 1%, bentonite 2%, and attapulgite clay q.s. to 100%.

Example 32

60% Polyhexamethylene Biguanide.Acibenzolar Wettable Powder

(37) polyhexamethylene biguanide 30%, acibenzolar 30%, an alkylpolyoxyethylene ether sulfonate 1%, nekal 2%, bentonite 1.5%, white carbon black 2%, and diatomaceous earth q.s. to 100%.

Example 33

78% Polyhexamethylene Biguanide Sulfate.Acibenzolar Wettable Powder

(38) polyhexamethylene biguanide sulfate 2%, acibenzolar 76%, an alkylsulfonate 2%, sodium lignin sulfonate 2%, white carbon black 3%, and kaolin q.s. to 100%.

Example 34

88% Polyhexamethylene Biguanide Hydrochloride.Dithianon Wettable Powder

(39) polyhexamethylene biguanide hydrochloride 86%, dithianon 2%, a polyoxyethylene octyl phenyl ether 2%, sodium lignin sulfonate 6%, white carbon black 4%, and diatomaceous earth q.s. to 100%.

Example 35

50% Polyhexamethylene Biguanide.Dithianon Wettable Powder

(40) polyhexamethylene biguanide 25%, dithianon 25%, calcium lignin sulfonate 7%, white carbon black 5%, sodium dodecylbenzene sulfonate 3%, and attapulgite clay q.s. to 100%.

Example 36

65% Polyhexamethylene Biguanide Acetate.Dithianon Wettable Powder

(41) polyhexamethylene biguanide acetate 2%, dithianon 63%, bentonite 5%, calcium lignin sulfonate 3%, a polyoxyethylene octyl phenyl ether 3%, and attapulgite clay q.s. to 100%.

Example 37

85% Polyhexamethylene Biguanide Hydrochloride.Pyraclostrobin Wettable Powder

(42) polyhexamethylene biguanide hydrochloride 83%, pyraclostrobin 2%, a polyoxyethylene octyl phenyl ether 1%, sodium lignin sulfonate 3%, white carbon black 6%, and diatomaceous earth q.s. to 100%.

Example 38

50% Polyhexamethylene Biguanide.Pyraclostrobin Wettable Powder

(43) polyhexamethylene biguanide 25%, pyraclostrobin 25%, sodium dodecylbenzene sulfonate 3%, white carbon black 8%, calcium lignin sulfonate 5%, and attapulgite clay q.s. to 100%.

Example 39

82% Polyhexamethylene Biguanide Stearate.Pyraclostrobin Wettable Powder

(44) polyhexamethylene biguanide stearate 2%, pyraclostrobin 80%, calcium lignin sulfonate 5%, bentonite 4%, a polyoxyethylene octyl phenyl ether 3%, and attapulgite clay q.s. to 100%.

Example 40

88% Polyhexamethylene Biguanide.Trifloxystrobin Wettable Powder

(45) polyhexamethylene biguanide 86%, trifloxystrobin 2%, sodium dodecylbenzene sulfonate 2%, bentonite 1%, calcium lignin sulfonate 2%, and attapulgite clay q.s. to 100%.

Example 41

50% Polyhexamethylene Biguanide Carbonate.Trifloxystrobin Wettable Powder

(46) polyhexamethylene biguanide carbonate 25%, trifloxystrobin 25%, sodium lignin sulfonate 6%, an alkylsulfonate 6%, white carbon black 11%, and kaolin q.s. to 100%.

Example 42

86% Polyhexamethylene Biguanide Hydrochloride.Trifloxystrobin Wettable Powder

(47) polyhexamethylene biguanide hydrochloride 2%, trifloxystrobin 84%, nekal 1%, an alkylpolyoxyethylene ether sulfonate 2%, bentonite 1.5%, white carbon black 2%, and diatomaceous earth q.s. to 100%.

Example 43

62% Polyhexamethylene Biguanide Hydrochloride.Picoxystrobin Wettable Powder

(48) polyhexamethylene biguanide hydrochloride 60%, picoxystrobin 2%, ammonium sulfate 1%, sodium carboxymethyl starch 3%, sodium dodecyl sulfonate 3%, sodium lignin sulfonate 2%, xanthan gum 1%, and attapulgite clay q.s. to 100%.

Example 44

40% Polyhexamethylene Biguanide Stearate.Picoxystrobin Wettable Powder

(49) polyhexamethylene biguanide stearate 20%, picoxystrobin 20%, sodium alginate 4%, ammonium sulfate 2%, a sodium methylnaphthalene sulfonate formaldehyde condensate 1%, organic silicone 1%, and bentonite q.s. to 100%.

Example 45

75% Polyhexamethylene Biguanide.Picoxystrobin Wettable Powder

(50) polyhexamethylene biguanide 2%, picoxystrobin 73%, a sodium methylnaphthalene sulfonate formaldehyde condensate 2%, sodium lignin sulfonate 5%, sodium dodecyl sulfate 3%, and diatomaceous earth q.s. to 100%.

Example 46

85% Polyhexamethylene Biguanide Hydrochloride.Fluazinam Wettable Powder

(51) polyhexamethylene biguanide hydrochloride 83%, fluazinam 2%, a polyoxyethylene octyl phenyl ether 1%, sodium lignin sulfonate 5%, white carbon black 3%, and diatomaceous earth q.s. to 100%.

Example 47

40% Polyhexamethylene Biguanide.Fluazinam Wettable Powder

(52) polyhexamethylene biguanide 20%, fluazinam 20%, calcium lignin sulfonate 5%, white carbon black 9%, sodium dodecylbenzene sulfonate 4%, and attapulgite clay q.s. to 100%.

Example 48

65% Polyhexamethylene Biguanide Acetate.Fluazinam Wettable Powder

(53) polyhexamethylene biguanide acetate 2%, fluazinam 63%, bentonite 4%, calcium lignin sulfonate 4%, a polyoxyethylene octyl phenyl ether 3%, and attapulgite clay q.s. to 100%.

Example 49

82% Polyhexamethylene Biguanide Hydrochloride.Thifluzamide Wettable Powder

(54) polyhexamethylene biguanide hydrochloride 80%, thifluzamide 2%, a polyoxyethylene octyl phenyl ether 1%, sodium lignin sulfonate 4%, white carbon black 5%, and diatomaceous earth q.s. to 100%.

Example 50

50% Polyhexamethylene Biguanide.Thifluzamide Wettable Powder

(55) polyhexamethylene biguanide 25%, thifluzamide 25%, sodium dodecylbenzene sulfonate 2%, white carbon black 5%, calcium lignin sulfonate 6%, and attapulgite clay q.s. to 100%.

Example 51

82% Polyhexamethylene Biguanide Stearate.Thifluzamide Wettable Powder

(56) polyhexamethylene biguanide stearate 2%, thifluzamide 80%, calcium lignin sulfonate 4%, bentonite 5%, a polyoxyethylene octyl phenyl ether 2%, and attapulgite clay q.s. to 100%.

Example 52

85% Polyhexamethylene Biguanide.Dimethomorph Wettable Powder

(57) polyhexamethylene biguanide 83%, dimethomorph 2%, sodium dodecylbenzene sulfonate 2%, bentonite 1%, calcium lignin sulfonate 2%, and attapulgite clay q.s. to 100%.

Example 53

50% Polyhexamethylene Biguanide Carbonate.Dimethomorph Wettable Powder

(58) polyhexamethylene biguanide carbonate 25%, dimethomorph 25%, sodium lignin sulfonate 4%, an alkylsulfonate 5%, white carbon black 10%, and kaolin q.s. to 100%.

Example 54

75% Polyhexamethylene Biguanide Hydrochloride.Dimethomorph Wettable Powder

(59) polyhexamethylene biguanide hydrochloride 2%, dimethomorph 73%, nekal 1%, an alkylpolyoxyethylene ether sulfonate 1%, bentonite 2%, white carbon black 3%, and diatomaceous earth q.s. to 100%.

(60) II. Efficacy Test

(61) (I) Bioassay Examples

(62) 1. Toxicity test of polyhexamethylene biguanide hydrochloride combined respectively with benthiavalicarb-isopropyl, dimethomorph, azoxystrobin, fenamidone, dithianon, polyoxin, zoxamide, acibenzolar, and fluazinam on downy mildew pathogens of cucumber

(63) Test target organism: downy mildew pathogens of cucumber

(64) Based on the test grade scale, the disease development on the leaves of the whole cucumber plant was investigated, and the disease index and control effect were calculated.

(65) The control effect was converted into probability (y), the concentration of the agents (g/ml) in solution was converted into a logarithmic value (x), the toxic regression equation and the median inhibition concentration EC50 were calculated by east square method, and the toxicity index and the co-toxicity coefficient (CTC) of the agents were calculated by SUN Peiyun method.
Actual toxicity index (ATI)=(EC50 of standard/EC50 of test agent)*100
Theoretical toxicity index (TTI)=toxicity index of agent A*percentage content of A in the mixture+toxicity index of agent B*percentage content of B in the mixture
Co-toxicity coefficient (CTC)=[actual toxicity index (ATI) of the mixture/theoretical toxicity index (TTI) of the mixture]*100

(66) Where CTC80, the composition exhibits an antagonistic effect; where 80<CTC<120, the composition exhibits an additive effect, and where CTC120, the composition exhibits a synergistic effect.

(67) (1) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Benthiavalicarb-Isopropyl on Downy Mildew Pathogens of Cucumber

(68) TABLE-US-00001 TABLE 1 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with benthiavalicarb-isopropyl on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 3.25 100.0 / / Benthiavalicarb-isopropyl 5.41 60.1 / / Polyhexamethylene biguanide 2.32 140.09 99.22 141.191 hydrochloride:benthiavalicarb-isopropyl = 50:1 Polyhexamethylene biguanide 1.91 170.16 98.67 172.454 hydrochloride:benthiavalicarb-isopropyl = 30:1 Polyhexamethylene biguanide 1.58 205.69 96.37 213.438 hydrochloride:benthiavalicarb-isopropyl = 10:1 Polyhexamethylene biguanide 2.08 156.25 80.05 195.191 hydrochloride:benthiavalicarb-isopropyl = 1:1 Polyhexamethylene biguanide 2.26 143.81 63.73 225.655 hydrochloride:benthiavalicarb-isopropyl = 1:10 Polyhexamethylene biguanide 3.79 85.75 61.43 139.589 hydrochloride:benthiavalicarb-isopropyl = 1:30 Polyhexamethylene biguanide 3.93 82.70 60.88 135.841 hydrochloride:benthiavalicarb-isopropyl = 1:50

(69) The results (in Table 1) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with benthiavalicarb-isopropyl on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber.

(70) (2) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Dimethomorph on Downy Mildew Pathogens of Cucumber

(71) TABLE-US-00002 TABLE 2 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with dimethomorph on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 4.08 100.0 / / Dimethomorph 6.36 63.9 / / Polyhexamethylene biguanide 2.99 136.5 99.29 137.48 hydrochloride:dimethomorph = 50:1 Polyhexamethylene biguanide 2.40 170.0 98.835 172.00 hydrochloride:dimethomorph = 30:1 Polyhexamethylene biguanide 2.12 192.4 96.718 198.93 hydrochloride:dimethomorph = 10:1 Polyhexamethylene biguanide 2.01 203.0 81.95 247.71 hydrochloride:dimethomorph = 1:1 Polyhexamethylene biguanide 2.91 140.2 67.18 208.69 hydrochloride:dimethomorph = 1:10 Polyhexamethylene biguanide 3.33 122.5 65.17 187.97 hydrochloride:dimethomorph = 1:30 Polyhexamethylene biguanide 4.87 83.8 64.60 129.72 hydrochloride:dimethomorph = 1:50

(72) The results (in Table 2) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with dimethomorph on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber. Especially when polyhexamethylene biguanide or an agriculturally acceptable salt thereof is mixed with dimethomorph at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and dimethomorph is always above 170, and the synergistic effect is obvious.

(73) (3) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Azoxystrobin on Downy Mildew Pathogens of Cucumber

(74) TABLE-US-00003 TABLE 3 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with azoxystrobin on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 4.21 100.00 / / Azoxystrobin 5.82 72.34 / / Polyhexamethylene biguanide 3.32 126.81 99.458 127.50 hydrochloride:azoxystrobin = 50:1 Polyhexamethylene biguanide 3.05 138.03 99.108 139.27 hydrochloride:azoxystrobin = 30:1 Polyhexamethylene biguanide 2.29 183.84 97.485 188.58 hydrochloride:azoxystrobin = 10:1 Polyhexamethylene biguanide 2.07 203.38 86.170 236.02 hydrochloride:azoxystrobin = 1:1 Polyhexamethylene biguanide 2.54 165.75 74.855 221.43 hydrochloride:azoxystrobin = 1:10 Polyhexamethylene biguanide 4.18 100.72 73.232 137.54 hydrochloride:azoxystrobin = 1:30 Polyhexamethylene biguanide 4.62 91.13 72.882 125.04 hydrochloride:azoxystrobin = 1:50

(75) The results (in Table 3) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with azoxystrobin on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber. Especially when polyhexamethylene biguanide hydrochloride is mixed with azoxystrobin at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and azoxystrobin is always above 135, and the synergistic effect is obvious.

(76) (4) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Fenamidone on Downy Mildew Pathogens of Cucumber

(77) TABLE-US-00004 TABLE 4 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with fenamidone on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.51 100 / / Fenamidone 5.12 107.62 / / Polyhexamethylene biguanide 4.49 122.72 100.149 122.537 hydrochloride:fenamidone = 50:1 Polyhexamethylene biguanide 4.13 133.56 100.246 133.232 hydrochloride:fenamidone = 30:1 Polyhexamethylene biguanide 3.69 149.35 100.693 148.322 hydrochloride:fenamidone = 10:1 Polyhexamethylene biguanide 2.69 205.09 103.81 197.563 hydrochloride:fenamidone = 1:1 Polyhexamethylene biguanide 2.72 202.57 106.927 189.447 hydrochloride:fenamidone = 1:10 Polyhexamethylene biguanide 3.9 141.28 107.374 131.577 hydrochloride:fenamidone = 1:30 Polyhexamethylene biguanide 4.23 130.26 107.471 121.205 hydrochloride:fenamidone = 1:50

(78) The results (in Table 4) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with fenamidone on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber. Especially when polyhexamethylene biguanide hydrochloride is mixed with fenamidone at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and fenamidone is always above 130, and the synergistic effect is obvious.

(79) (5) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Dithianon on Downy Mildew Pathogens of Cucumber

(80) TABLE-US-00005 TABLE 5 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with dithianon on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 4.72 100 / / Dithianon 5.34 88.39 / / Polyhexamethylene biguanide 4.06 116.26 99.772 116.526 hydrochloride:dithianon = 50:1 Polyhexamethylene biguanide 3.69 127.91 99.625 128.391 hydrochloride:dithianon = 30:1 Polyhexamethylene biguanide 2.83 166.78 98.945 168.558 hydrochloride:dithianon = 10:1 Polyhexamethylene biguanide 2.92 161.64 94.195 171.601 hydrochloride:dithianon = 1:1 Polyhexamethylene biguanide 2.83 166.78 89.445 186.461 hydrochloride:dithianon = 1:10 Polyhexamethylene biguanide 4.06 116.26 88.765 130.975 hydrochloride:dithianon = 1:30 Polyhexamethylene biguanide 4.38 107.76 88.618 121.601 hydrochloride:dithianon = 1:50

(81) The results (in Table 5) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with dithianon on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber. Especially when polyhexamethylene biguanide or an agriculturally acceptable salt thereof is mixed with dithianon at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and dithianon is always above 125, and the synergistic effect is obvious.

(82) (6) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Polyoxin on Downy Mildew Pathogens of Cucumber

(83) TABLE-US-00006 TABLE 6 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with polyoxin on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 4.71 100 / / Polyoxin 5.24 89.89 / / Polyhexamethylene biguanide 4.01 117.46 99.802 117.689 hydrochloride:polyoxin = 50:1 Polyhexamethylene biguanide 3.80 123.95 99.674 124.353 hydrochloride:polyoxin = 30:1 Polyhexamethylene biguanide 3.49 134.96 99.081 136.209 hydrochloride:polyoxin = 10:1 Polyhexamethylene biguanide 2.99 157.53 94.945 165.912 hydrochloride:polyoxin = 1:1 Polyhexamethylene biguanide 3.32 141.87 90.809 156.226 hydrochloride:polyoxin = 1:10 Polyhexamethylene biguanide 4.3 109.53 90.216 121.414 hydrochloride:polyoxin = 1:30 Polyhexamethylene biguanide 4.53 103.97 90.088 115.413 hydrochloride:polyoxin = 1:50

(84) The results (in Table 6) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with polyoxin on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber.

(85) (7) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Zoxamide on Downy Mildew Pathogens of Cucumber

(86) TABLE-US-00007 TABLE 7 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with zoxamide on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 4.89 100 / / Zoxamide 5.11 95.69 / / Polyhexamethylene biguanide 4.19 116.71 99.915 116.806 hydrochloride:zoxamide = 50:1 Polyhexamethylene biguanide 4.05 120.74 99.861 120.909 hydrochloride:zoxamide = 30:1 Polyhexamethylene biguanide 3.39 144.25 99.608 144.815 hydrochloride:zoxamide = 10:1 Polyhexamethylene biguanide 3.19 153.29 97.845 156.668 hydrochloride:zoxamide = 1:1 Polyhexamethylene biguanide 3.42 142.98 96.082 148.813 hydrochloride:zoxamide = 1:10 Polyhexamethylene biguanide 4.02 121.64 95.829 126.936 hydrochloride:zoxamide = 1:30 Polyhexamethylene biguanide 4.16 117.55 95.775 122.734 hydrochloride:zoxamide = 1:50

(87) The results (in Table 7) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with zoxamide on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber. Especially when polyhexamethylene biguanide hydrochloride is mixed with zoxamide at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and zoxamide is always above 130, and the synergistic effect is obvious.

(88) (8) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Acibenzolar on Downy Mildew Pathogens of Cucumber

(89) TABLE-US-00008 TABLE 8 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with acibenzolar on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 4.92 100 / / Acibenzolar 5.38 91.45 / / Polyhexamethylene biguanide 4.21 116.865 99.832 117.062 hydrochloride:acibenzolar = 50:1 Polyhexamethylene biguanide 4.03 122.084 99.724 122.422 hydrochloride:acibenzolar = 30:1 Polyhexamethylene biguanide 3.13 157.188 99.223 158.419 hydrochloride:acibenzolar = 10:1 Polyhexamethylene biguanide 3.19 154.232 95.725 161.120 hydrochloride:acibenzolar = 1:1 Polyhexamethylene biguanide 3.32 148.193 92.227 160.683 hydrochloride:acibenzolar = 1:10 Polyhexamethylene biguanide 3.87 127.132 91.726 138.600 hydrochloride:acibenzolar = 1:30 Polyhexamethylene biguanide 4.53 108.609 91.618 118.545 hydrochloride:acibenzolar = 1:50

(90) The results (in Table 8) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with acibenzolar on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber. Especially when polyhexamethylene biguanide hydrochloride is mixed with acibenzolar at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and acibenzolar is always above 130, and the synergistic effect is obvious.

(91) (9) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Fluazinam on Downy Mildew Pathogens of Cucumber

(92) TABLE-US-00009 TABLE 9 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with fluazinam on downy mildew pathogens of cucumber Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.08 100 / / Fluazinam 5.19 97.88 / / Polyhexamethylene biguanide 4.19 121.241 99.958 121.292 hydrochloride:fluazinam = 50:1 Polyhexamethylene biguanide 4.03 126.055 99.932 126.141 hydrochloride:fluazinam = 30:1 Polyhexamethylene biguanide 3.5 145.143 99.807 145.424 hydrochloride:fluazinam = 10:1 Polyhexamethylene biguanide 3.09 164.401 98.94 166.162 hydrochloride:fluazinam = 1:1 Polyhexamethylene biguanide 3.29 154.407 98.073 157.441 hydrochloride:fluazinam = 1:10 Polyhexamethylene biguanide 3.91 129.923 97.948 132.645 hydrochloride:fluazinam = 1:30 Polyhexamethylene biguanide 4.47 113.647 97.922 116.059 hydrochloride:fluazinam = 1:50

(93) The results (in Table 9) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with fluazinam on downy mildew of cucumber is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew pathogens of cucumber. Especially when polyhexamethylene biguanide hydrochloride is mixed with fluazinam at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and fluazinam is always above 120, and the synergistic effect is obvious.

(94) 2. Toxicity test of polyhexamethylene biguanide hydrochloride combined respectively with difenoconazole, epoxiconazole, prothioconazole, pyraclostrobin, trifloxystrobin, picoxystrobin, thifluzamide, iprodione, and cyprodinil on rust pathogens of wheat. The test method was the same as above.

(95) (1) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Difenoconazole on Rust Pathogens of Wheat

(96) TABLE-US-00010 TABLE 10 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with difenoconazole on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.82 100 / / Difenoconazole 5.45 106.79 / / Polyhexamethylene biguanide 4.92 118.293 100.133 118.136 hydrochloride:difenoconazole = 50:1 Polyhexamethylene biguanide 4.73 123.044 100.219 122.776 hydrochloride:difenoconazole = 30:1 Polyhexamethylene biguanide 3.52 165.341 100.617 164.327 hydrochloride:difenoconazole = 10:1 Polyhexamethylene biguanide 3.12 186.538 103.395 180.413 hydrochloride:difenoconazole = 1:1 Polyhexamethylene biguanide 3.41 170.674 106.173 160.751 hydrochloride:difenoconazole = 1:10 Polyhexamethylene biguanide 4.16 139.904 106.571 131.278 hydrochloride:difenoconazole = 1:30 Polyhexamethylene biguanide 4.90 118.776 106.657 111.362 hydrochloride:difenoconazole = 1:50

(97) The results (in Table 10) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with difenoconazole on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with difenoconazole at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and difenoconazole is always above 120, and the synergistic effect is obvious.

(98) (2) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Epoxiconazole on Rust Pathogens of Wheat

(99) TABLE-US-00011 TABLE 11 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with epoxiconazole on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.69 100 / / Epoxiconazole 5.29 107.56 / / Polyhexamethylene biguanide 4.85 117.32 100.148 117.146 hydrochloride:epoxiconazole = 50:1 Polyhexamethylene biguanide 4.65 122.37 100.244 122.068 hydrochloride:epoxiconazole = 30:1 Polyhexamethylene biguanide 3.42 166.37 100.687 165.239 hydrochloride:epoxiconazole = 10:1 Polyhexamethylene biguanide 3.04 187.17 103.78 180.354 hydrochloride:epoxiconazole = 1:1 Polyhexamethylene biguanide 3.39 167.85 106.873 157.052 hydrochloride:epoxiconazole = 1:10 Polyhexamethylene biguanide 4.11 138.44 107.316 129.005 hydrochloride:epoxiconazole = 1:30 Polyhexamethylene biguanide 4.76 119.54 107.412 111.289 hydrochloride:epoxiconazole = 1:50

(100) The results (in Table 11) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with epoxiconazole on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with epoxiconazole at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and epoxiconazole is always above 120, and the synergistic effect is obvious.

(101) (3) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Prothioconazole on Rust Pathogens of Wheat

(102) TABLE-US-00012 TABLE 12 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with prothioconazole on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.59 100 / / Prothioconazole 5.12 109.18 / / Polyhexamethylene biguanide 4.91 113.85 100.18 113.645 hydrochloride:prothioconazole = 50:1 Polyhexamethylene biguanide 4.33 129.10 100.296 128.718 hydrochloride:prothioconazole = 30:1 Polyhexamethylene biguanide 3.37 165.88 100.835 164.502 hydrochloride:prothioconazole = 10:1 Polyhexamethylene biguanide 3.11 179.74 104.59 171.855 hydrochloride:prothioconazole = 1:1 Polyhexamethylene biguanide 3.41 163.93 108.345 151.303 hydrochloride:prothioconazole = 1:10 Polyhexamethylene biguanide 4.04 138.37 108.884 127.077 hydrochloride:prothioconazole = 1:30 Polyhexamethylene biguanide 4.69 119.19 109.000 109.348 hydrochloride:prothioconazole = 1:50

(103) The results (in Table 12) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with prothioconazole on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with prothioconazole at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and prothioconazole is always above 120, and the synergistic effect is obvious.

(104) (4) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Pyraclostrobin on Rust Pathogens of Wheat

(105) TABLE-US-00013 TABLE 13 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with pyraclostrobin on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.45 100 / / Pyraclostrobin 4.98 109.44 / / Polyhexamethylene biguanide 4.83 112.84 100.185 112.628 hydrochloride:pyraclostrobin = 50:1 Polyhexamethylene biguanide 4.24 128.54 100.305 128.147 hydrochloride:pyraclostrobin = 30:1 Polyhexamethylene biguanide 3.46 157.51 100.858 156.174 hydrochloride:pyraclostrobin = 10:1 Polyhexamethylene biguanide 3.03 179.87 104.72 171.761 hydrochloride:pyraclostrobin = 1:1 Polyhexamethylene biguanide 3.51 155.27 108.582 142.999 hydrochloride:pyraclostrobin = 1:10 Polyhexamethylene biguanide 4.11 132.60 109.135 121.504 hydrochloride:pyraclostrobin = 1:30 Polyhexamethylene biguanide 4.57 119.26 109.255 109.154 hydrochloride:pyraclostrobin = 1:50

(106) The results (in Table 13) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with pyraclostrobin on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with pyraclostrobin at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and pyraclostrobin is always above 120, and the synergistic effect is obvious.

(107) (5) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Trifloxystrobin on Rust Pathogens of Wheat

(108) TABLE-US-00014 TABLE 14 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with trifloxystrobin on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.12 100 / / Trifloxystrobin 4.75 107.79 / / Polyhexamethylene biguanide 4.65 110.11 100.153 109.939 hydrochloride:trifloxystrobin = 50:1 Polyhexamethylene biguanide 4.12 124.27 100.251 123.961 hydrochloride:trifloxystrobin = 30:1 Polyhexamethylene biguanide 3.29 155.62 100.708 154.529 hydrochloride:trifloxystrobin = 10:1 Polyhexamethylene biguanide 3.03 168.98 103.895 162.642 hydrochloride:trifloxystrobin = 1:1 Polyhexamethylene biguanide 3.25 157.54 107.082 147.119 hydrochloride:trifloxystrobin = 1:10 Polyhexamethylene biguanide 3.91 130.95 107.539 121.766 hydrochloride:trifloxystrobin = 1:30 Polyhexamethylene biguanide 4.07 125.80 107.637 116.873 hydrochloride:trifloxystrobin = 1:50

(109) The results (in Table 14) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with trifloxystrobin on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with trifloxystrobin at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and trifloxystrobin is always above 120, and the synergistic effect is obvious.

(110) (6) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Picoxystrobin on Rust Pathogens of Wheat

(111) TABLE-US-00015 TABLE 15 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with picoxystrobin on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 4.98 100 / / Picoxystrobin 5.68 87.68 / / Polyhexamethylene biguanide 4.43 112.42 99.758 112.693 hydrochloride:picoxystrobin = 50:1 Polyhexamethylene biguanide 4.02 123.88 99.603 124.374 hydrochloride:picoxystrobin = 30:1 Polyhexamethylene biguanide 3.1 160.65 98.88 162.470 hydrochloride:picoxystrobin = 10:1 Polyhexamethylene biguanide 3.04 163.82 93.84 174.574 hydrochloride:picoxystrobin = 1:1 Polyhexamethylene biguanide 3.19 156.11 88.80 175.800 hydrochloride:picoxystrobin = 1:10 Polyhexamethylene biguanide 4.54 109.69 88.077 124.539 hydrochloride:picoxystrobin = 1:30 Polyhexamethylene biguanide 4.7 105.96 87.922 120.516 hydrochloride:picoxystrobin = 1:50

(112) The results (in Table 15) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with picoxystrobin on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with picoxystrobin at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and picoxystrobin is always above 120, and the synergistic effect is obvious.

(113) (7) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Thifluzamide on Rust Pathogens of Wheat

(114) TABLE-US-00016 TABLE 16 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with thifluzamide on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.56 100 / / Thifluzamide 5.12 108.59 / / Polyhexamethylene biguanide 4.78 116.32 100.168 116.125 hydrochloride:thifluzamide = 50:1 Polyhexamethylene biguanide 4.28 129.91 100.277 129.551 hydrochloride:thifluzamide = 30:1 Polyhexamethylene biguanide 3.22 172.67 100.781 171.332 hydrochloride:thifluzamide = 10:1 Polyhexamethylene biguanide 3.3 168.48 104.295 161.542 hydrochloride:thifluzamide = 1:1 Polyhexamethylene biguanide 3.08 180.52 107.809 167.444 hydrochloride:thifluzamide = 1:10 Polyhexamethylene biguanide 4.18 133.01 108.313 122.802 hydrochloride:thifluzamide = 1:30 Polyhexamethylene biguanide 4.36 127.52 108.422 117.615 hydrochloride:thifluzamide = 1:50

(115) The results (in Table 16) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with thifluzamide on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with thifluzamide at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and thifluzamide is always above 120, and the synergistic effect is obvious.

(116) (8) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Iprodione on Rust Pathogens of Wheat

(117) TABLE-US-00017 TABLE 17 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with iprodione on rust pathogens of wheat Co-toxicity EC.sub.50 coefficient Name of agent (g/ml) ATI TTI (CTC) Polyhexamethylene biguanide hydrochloride 5.89 100.00 / / Iprodione 5.35 110.09 / / Polyhexamethylene biguanide 4.98 118.27 100.198 118.036 hydrochloride:iprodione = 50:1 Polyhexamethylene biguanide 4.46 132.06 100.325 131.632 hydrochloride:iprodione = 30:1 Polyhexamethylene biguanide 3.83 153.79 100.917 152.393 hydrochloride:iprodione = 10:1 Polyhexamethylene biguanide 3.32 177.41 105.045 168.890 hydrochloride:iprodione = 1:1 Polyhexamethylene biguanide 3.11 189.39 109.173 173.477 hydrochloride:iprodione = 1:10 Polyhexamethylene biguanide 4.20 140.24 109.765 127.764 hydrochloride:iprodione = 1:30 Polyhexamethylene biguanide 4.88 120.70 109.892 109.835 hydrochloride:iprodione = 1:50

(118) The results (in Table 17) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with iprodione on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with iprodione at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and iprodione is always above 120, and the synergistic effect is obvious.

(119) (9) Toxicity Test of Polyhexamethylene Biguanide Hydrochloride Combined with Cyprodinil on Rust Pathogens of Wheat

(120) TABLE-US-00018 TABLE 18 Toxicity test result analysis of polyhexamethylene biguanide hydrochloride combined with cyprodinil on rust pathogens of wheat EC.sub.50 Co-toxicity (g/ coefficient Name of agent ml) ATI TTI (CTC) Polyhexamethylene biguanide 5.25 100 / / hydrochloride Cyprodinil 5.87 89.44 / / Polyhexamethylene biguanide 4.83 108.70 99.793 108.925 hydrochloride:cyprodinil = 50:1 Polyhexamethylene biguanide 4.36 120.41 99.659 120.822 hydrochloride:cyprodinil = 30:1 Polyhexamethylene biguanide 3.53 148.73 99.04 150.172 hydrochloride:cyprodinil = 10:1 Polyhexamethylene biguanide 3.22 163.04 94.72 172.128 hydrochloride:cyprodinil = 1:1 Polyhexamethylene biguanide 3.51 149.57 90.4 165.454 hydrochloride:cyprodinil = 1:10 Polyhexamethylene biguanide 4.65 112.90 89.781 125.750 hydrochloride:cyprodinil = 1:30 Polyhexamethylene biguanide 4.99 105.21 89.647 117.360 hydrochloride:cyprodinil = 1:50

(121) The results (in Table 18) show that the control effect of the combination of polyhexamethylene biguanide hydrochloride with cyprodinil on rust of wheat is significantly improved, suggesting that the combination has an obvious synergistic effect on rust pathogens of wheat. Especially when polyhexamethylene biguanide hydrochloride is mixed with cyprodinil at a ratio ranging from 1:30 to 30:1, the co-toxicity coefficient of polyhexamethylene biguanide hydrochloride and cyprodinil is always above 120, and the synergistic effect is obvious.

(122) (II) Field Efficacy Test

(123) Test method: in early stage of disease development, the first spray was given immediately, and then the second application was given after 7 days. Each treatment included 4 plots of 20 square meters each. The disease development before application and 10 days after the second application was statistically investigated. Samples were collected from 5 locations in each plot at random, and 5 plants were investigated at each location by investigating the percentages of the disease spot area on the leaves relative to the leaf area of the whole plant and grading. The disease index and the control effect were calculated.

(124) $\mathrm{Disease}\mathrm{index}=\frac{.Math.(\begin{array}{c}\mathrm{Number}\mathrm{of}\mathrm{leaves}\mathrm{at}\mathrm{each}\mathrm{grade}\mathrm{of}\mathrm{disease}\mathrm{development}\\ \mathrm{Representative}\mathrm{value}\mathrm{of}\mathrm{corresponding}\mathrm{grade}\end{array})}{\begin{array}{c}\mathrm{Total}\mathrm{number}\mathrm{of}\mathrm{leaves}\mathrm{investigated}\\ \mathrm{Representative}\mathrm{value}\mathrm{of}\mathrm{highest}\mathrm{level}\end{array}}100$$\mathrm{Control}\mathrm{effect}\left(\%\right)=\left(1-\frac{\begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{control}\mathrm{group}\mathrm{before}\mathrm{application}\\ \mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{treatment}\mathrm{group}\mathrm{after}\mathrm{application}\end{array}}{\begin{array}{c}\mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{control}\mathrm{group}\mathrm{after}\mathrm{application}\\ \mathrm{Disease}\mathrm{index}\mathrm{of}\mathrm{treatment}\mathrm{group}\mathrm{before}\mathrm{application}\end{array}}\right)100$
Anticipated control effect (%)=X+YXY/100 (where X and Y are the control effect of a single agent)

(125) Grade scale:

(126) Grade 0: no disease spot;

(127) Grade 1: number of disease spots on the leaf<5, and length<1 cm;

(128) Grade 3: 6number of disease spots on the leaf10, and length of some disease spots>1 cm;

(129) Grade 5: 11number of disease spots on the leaf25, some disease spots are contiguous, and the disease spot area is 10-25% of the leaf area;

(130) Grade 7: number of disease spots on the leaf26, the disease spots are contiguous, and the disease spot area is 26-50% of the leaf area;

(131) Grade 9: the disease spots are contiguous, and the disease spot area is above 50% of the leaf area, or all the leaves all wilted.

(132) 1. Field Efficacy Test of Polyhexamethylene Biguanide or a Salt Thereof Combined Respectively with Cyprodinil (or a Salt Thereof), Trifloxystrobin, Picoxystrobin, Fluazinam, and Thifluzamide for Controlling Rice Blast

(133) TABLE-US-00019 TABLE 19 Field efficacy test of polyhexamethylene biguanide or a salt thereof combined with the above fungicides for rice blast Disease Day 11 after the Application index second application rate before Disease Control No. Treatment agent (a.i.g/ha) application index effect (%) Example 20% polyhexamethylene biguanide 116 2.79 4.02 84.5 1 aqueous solution 50% cyprodinil wettable powder 4 3.21 28.65 4.1 Anticipated control effect after mixing 85.1 them 62% polyhexamethylene biguanide 120 3.02 3.60 87.2 hydrochloridecyprodinil wettable powder (polyhexamethylene biguanide hydrochloride:cyprodinil = 60:2) Example 20% polyhexamethylene biguanide 60 2.81 11.19 57.2 2 aqueous solution 50% cyprodinil wettable powder 60 2.9 18.59 31.1 Anticipated control effect after mixing 70.5 them 50% polyhexamethylene biguanide 120 2.88 2.89 89.2 acetatecyprodinil wettable powder (polyhexamethylene biguanide acetate:cyprodinil = 25:25) Example 20% polyhexamethylene biguanide 3.7 2.69 22.98 8.2 3 aqueous solution 50% cyprodinil wettable powder 116.3 2.79 5.58 78.5 Anticipated control effect after mixing 80.3 them 65% polyhexamethylene biguanide 120 2.72 3.09 87.8 hydrochloridecyprodinil wettable powder (polyhexamethylene biguanide hydrochloride:cyprodinil = 2:63) Example 20% polyhexamethylene biguanide 117 2.85 4.06 84.7 40 aqueous solution 25% trifloxystrobin wettable powder 3 2.98 26.48 4.5 Anticipated control effect after mixing 85.4 them 88% polyhexamethylene biguanidetri- 120 2.83 3.21 87.8 floxystrobin wettable powder (polyhexamethylene biguanide hydrochloride:Trifloxystrobin = 86:2) Example 20% polyhexamethylene biguanide 60 2.76 11.07 56.9 41 aqueous solution 25% trifloxystrobin wettable powder 60 3.04 15.93 43.7 Anticipated control effect after mixing 75.7 them 50% polyhexamethylene biguanide 120 2.93 3.57 86.9 carbonatetrifloxystrobin wettable powder (polyhexamethylene biguanide carbonate:trifloxystrobin = 25:25) Example 20% polyhexamethylene biguanide 2.8 3 26.02 6.8 42 aqueous solution 25% trifloxystrobin wettable powder 117.2 2.87 5.47 79.5 Anticipated control effect after mixing 80.9 them 86% polyhexamethylene biguanide 120 2.91 3.44 87.3 hydrochloridetrifloxystrobin wettable powder (polyhexamethylene biguanide hydrochloride:triflioxystrobin = 2:84) Example 20% polyhexamethylene biguanide 116 2.79 4.13 84.1 43 aqueous solution 25% picoxystrobin wettable powder 4 2.85 25.49 3.9 Anticipated control effect after mixing 84.7 them 62% polyhexamethylene biguanide 120 3.02 3.23 88.5 hydrochloridepicoxystrobin wettable powder (polyhexamethylene biguanide hydrochloride:picoxystrobin = 60:2) Example 20% polyhexamethylene biguanide 60 2.68 10.45 58.1 44 aqueous solution 25% picoxystrobin wettable powder 60 2.89 14.01 47.9 Anticipated control effect after mixing 78.2 them 40% polyhexamethylene biguanide 120 2.75 3.10 87.9 stearatepicoxystrobin wettable powder (polyhexamethylene biguanide stearate:picoxystrobin20:20) Example 20% polyhexamethylene biguanide 3.2 2.87 24.86 6.9 45 aqueous solution 25% picoxystrobin wettable powder 116.8 2.93 5.04 81.5 Anticipated control effect after mixing them 82.8 75% polyhexamethylene biguanidepic- 120 2.85 3.50 86.8 oxystrobin wettable powder (polyhexamethylene biguanide:picoxystrobin = 2:73) Example 20% polyhexamethylene biguanide 117 2.68 3.92 84.3 46 aqueous solution 15% fluazinam wettable powder 3 2.89 25.68 4.5 Anticipated control effect after mixing 85.0 them 85% polyhexamethylene biguanide 120 2.75 3.10 87.9 hydrochloridefluazinam wettable powder (polyhexamethylene biguanide hydrochloride:fluazinam = 83:2) Example 20% polyhexamethylene biguanide 60 2.76 10.89 57.6 47 aqueous solution 15% fluazinam wettable powder 60 2.85 13.23 50.1 Anticipated control effect after mixing 78.8 them 40% polyhexamethylene biguanideFluazinam 120 3.11 2.95 89.8 wettable powder (polyhexamethylene biguanide:fluazinam = 20:20) Example 20% polyhexamethylene biguanide 3.7 2.9 24.93 7.6 48 aqueous solution 15% fluazinam wettable powder 116.3 2.85 4.72 82.2 Anticipated control effect after mixing 83.6 them 65% polyhexamethylene biguanide 120 2.79 2.88 88.9 acetatefluazinam wettable powder (polyhexamethylene biguanide acetate:fluazinam = 2:63) Example 20% polyhexamethylene biguanide 117 3.21 5.02 83.2 49 aqueous solution 20% thifluzamide wettable powder 3 3.16 27.90 5.1 Anticipated control effect after mixing 84.1 them 82% polyhexamethylene biguanide 120 3.29 3.77 87.7 hydrochloridethifluzamide wettable powder (polyhexamethylene biguanide hydrochloride:thifluzamide = 80:2) Example 20% polyhexamethylene biguanide 60 3.05 11.86 58.2 50 aqueous solution 20% thifluzamide wettable powder 60 3.25 13.28 56.1 Anticipated control effect after mixing 81.6 them 50% polyhexamethylene 120 2.99 3.48 87.5 biguanidethifluzamide wettable powder (polyhexamethylene biguanide:thifluzamide = 25:25) Example 20% polyhexamethylene biguanide 3 3.3 28.62 6.8 51 aqueous solution 20% thifluzamide wettable powder 117 3.31 5.51 82.1 Anticipated control effect after mixing 83.3 them 82% polyhexamethylene biguanide 120 3.12 3.51 87.9 stearatethifluzamide wettable powder (polyhexamethylene biguanide stearate:thifluzamide = 2:80) Water 2.85 26.52 control (CK)

(134) The test results (in Table 19) show that the control effect of the combination of polyhexamethylene biguanide or a salt thereof with cyprodinil (or a salt thereof), trifloxystrobin, picoxystrobin, fluazinam, and thifluzamide respectively on rice blast is significantly improved, suggesting that the combination has an obvious synergistic effect on rice blast.

(135) (2) Efficacy Test of Polyhexamethylene Biguanide or a Salt Thereof Combined Respectively with Epoxiconazole, Prothioconazole, Difenoconazole, Polyoxin, and Iprodione for Rice Sheath Blight

(136) TABLE-US-00020 TABLE 20 Efficacy test of polyhexamethylene biguanide or a salt thereof combined respectively with the above fungicides for rice sheath blight Disease Day 11 after the Application index second application rate before Disease Control No. Treatment agent (a.i.g/ha) application index effect (%) Example 20% polyhexamethylene biguanide 117 2.99 3.76 83.1 4 aqueous solution 12.6% epoxiconazole suspension 3 3.32 23.75 3.9 Anticipated control effect after 83.8 mixing them 85% polyhexamethylene biguanide 120 3.12 3.04 86.9 hydrochlorideepoxiconazole wettable powder (polyhexamethylene biguanide hydrochloride:epoxiconazole = 83:2) Example 20% polyhexamethylene biguanide 60 3.21 10.68 55.3 5 aqueous solution 12.6% epoxiconazole suspension 60 3.32 14.11 42.9 Anticipated control effect after 74.5 mixing them 30% polyhexamethylene biguanide 120 3.34 2.91 88.3 acetateepoxiconazole wettable powder (polyhexamethylene biguanide acetate:epoxiconazole = 15:15) Example 20% polyhexamethylene biguanide 3.2 2.92 20.19 7.1 6 aqueous solution 12.6% epoxiconazole suspension 116.8 2.87 5.08 76.2 Anticipated control effect after 77.9 mixing them 75% polyhexamethylene biguanide 120 2.98 3.04 86.3 carbonateepoxiconazole wettable powder (polyhexamethylene biguanide carbonate:epoxiconazole = 2:73) Example 20% polyhexamethylene biguanide 117 2.76 3.39 83.5 16 aqueous solution 20% prothioconazole wettable 3 2.92 20.82 4.2 powder Anticipated control effect after 84.2 mixing them 85% polyhexamethylene biguanide 120 2.79 2.64 87.3 hydrochlorideprothioconazole wettable powder (polyhexamethylene biguanide hydrochloride:prothioconazole = 83:2) Example 20% polyhexamethylene biguanide 60 2.98 9.94 55.2 17 aqueous solution 20% prothioconazole wettable 60 3.32 12.75 48.4 powder Anticipated control effect after 76.9 mixing them 50% polyhexamethylene 120 2.99 2.63 88.2 biguanideprothioconazole wettable powder (polyhexamethylene biguanide:prothioconazole = 25:25) Example 20% polyhexamethylene biguanide 2.8 3.21 22.43 6.1 18 aqueous solution 20% prothioconazole wettable 117.2 2.87 5.08 76.2 powder Anticipated control effect after 77.7 mixing them 85% polyhexamethylene biguanide 120 3.12 3.07 86.8 stearateprothioconazole wettable powder (polyhexamethylene biguanide stearate:prothioconazole = 2:83) Example 20% polyhexamethylene biguanide 117.3 3.42 3.89 84.7 19 aqueous solution 18% difenoconazole suspension 2.8 3.42 24.64 3.2 Anticipated control effect after 85.2 mixing them 88% polyhexamethylene biguanidedi- 120 3.32 3.19 87.1 fenoconazole wettable powder (polyhexamethylene biguanide:difenoconazole = 86:2) Example 20% polyhexamethylene biguanide 60 3.62 11.80 56.2 20 aqueous solution 18% difenoconazole suspension 60 3.23 12.07 49.8 Anticipated control effect after 78.0 mixing them 50% polyhexamethylene biguanide 120 3.25 3.31 86.3 carbonatedifenoconazole wettable powder (polyhexamethylene biguanide carbonate:difenoconazole = 25:25) Example 20% polyhexamethylene biguanide 2.8 2.97 20.67 6.5 21 aqueous solution 18% difenoconazole suspension 117.2 2.99 3.65 83.6 Anticipated control effect after 84.7 mixing them 86% polyhexamethylene biguanide 120 3.15 2.98 87.3 hydrochloridedifenoconazole wettable powder (polyhexamethylene biguanide hydrochloride:difenoconazole = 2:84) Example 20% polyhexamethylene biguanide 117.2 3.28 4.03 83.5 25 aqueous solution 20% polyoxin wettable powder 2.8 3.32 23.77 3.8 Anticipated control effect after 84.1 mixing them 85% polyhexamethylene biguanide 120 2.75 2.42 88.2 hydrochloridepolyoxin wettable powder (polyhexamethylene biguanide hydrochloride:polyoxin = 83:2) Example 20% polyhexamethylene biguanide 60 2.76 9.00 56.2 26 aqueous solution 20% polyoxin wettable powder 60 2.85 9.99 52.9 Anticipated control effect after 79.4 mixing them 30% polyhexamethylene biguanide 120 3.31 2.59 89.5 acetatepolyoxin wettable powder (polyhexamethylene biguanide acetate:polyoxin = 15:15) Example 20% polyhexamethylene biguanide 3.2 2.87 19.76 7.5 27 aqueous solution 20% polyoxin wettable powder 116.8 2.79 3.22 84.5 Anticipated control effect after 85.7 mixing them 75% polyhexamethylene biguanide 120 2.98 2.71 87.8 carbonatepolyoxin wettable powder (polyhexamethylene biguanide carbonate:polyoxin = 2:73) Example 20% polyhexamethylene biguanide 116.1 3.43 4.42 82.7 28 aqueous solution 50% iprodione suspension 3.9 3.11 21.80 5.8 Anticipated control effect after 83.7 mixing them 62% polyhexamethylene biguanide 120 3.65 3.75 86.2 hydrochlorideiprodione wettable powder (polyhexamethylene biguanide hydrochloride:iprodione = 60:2) Example 20% polyhexamethylene biguanide 60 3.23 10.55 56.1 29 aqueous solution 50% iprodione suspension 60 2.95 10.06 54.2 Anticipated control effect after 79.9 mixing them 40% polyhexamethylene biguanide 120 2.98 2.42 89.1 stearateiprodione wettable powder (polyhexamethylene biguanide stearate:iprodione = 20:20) Example 20% polyhexamethylene biguanide 3.2 3.12 21.85 5.9 30 aqueous solution 50% iprodione suspension 116.8 3.32 4.55 81.6 Anticipated control effect after 82.7 mixing them 75% polyhexamethylene 120 3.34 3.33 86.6 biguanideiprodione wettable powder (polyhexamethylene biguanide:iprodione = 2:73) Water 3.21 23.89 control (CK)

(137) The test results (in Table 20) show that the control effect of the combination of polyhexamethylene biguanide or a salt thereof with epoxiconazole, prothioconazole, difenoconazole, polyoxin, and iprodione respectively on sheath blight of rice is significantly improved, suggesting that the combination has an obvious synergistic effect on rice sheath blight.

(138) (3) Efficacy Test of Polyhexamethylene Biguanide or a Salt Thereof Combined Respectively with Benthiavalicarb-Isopropyl, Zoxamide, Azoxystrobin, Fenamidone, Acibenzolar, Dithianon, Pyraclostrobin, and Dimethomorph for Downy Mildew of Grape

(139) TABLE-US-00021 TABLE 21 Efficacy test of polyhexamethylene biguanide or a salt thereof combined respectively with the above fungicides for downy mildew of grape Disease Day 11 after the Application index second application rate before Disease Control No. Treatment agent (a.i.g/ha) application index effect (%) Example 20% polyhexamethylene biguanide 130.6 3.75 4.61 82.6 7 aqueous solution 25% benthiavalicarb-isopropyl 4.4 3.92 26.51 4.3 wettable powder Anticipated control effect after 83.3 mixing them 62% polyhexamethylene biguanide 135 3.87 3.69 86.5 hydrochloridebenthiavalicarb-isopropyl wettable powder (polyhexamethylene biguanide hydrochloride:benthiavalicarb-isopropyl = 60:2) Example 20% polyhexamethylene biguanide 67.5 3.96 15.03 46.3 8 aqueous solution 25% benthiavalicarb-isopropyl 67.5 3.89 16.91 38.5 wettable powder Anticipated control effect after 67.0 mixing them 40% polyhexamethylene biguanide 135 3.98 3.35 88.1 stearatebenthiavalicarb-isopropyl wettable powder (polyhexamethylene biguanide stearate:benthiavalicarb-isopropyl = 20:20) Example 20% polyhexamethylene biguanide 3.6 3.89 25.26 8.1 9 aqueous solution 25% benthiavalicarb-isopropyl 126.4 3.82 6.16 77.2 wettable powder Anticipated control effect after 79.0 mixing them 75% polyhexamethylene 135 3.72 3.60 86.3 biguanidebenthiavalicarb-isopropyl wettable powder (polyhexamethylene biguanide:benthiavalicarb-isopropyl = 2:73) Example 20% polyhexamethylene biguanide 130.2 3.89 4.65 83.1 10 aqueous solution 30% zoxamide wettable powder 4.8 3.98 26.83 4.6 Anticipated control effect after 83.9 mixing them 85% polyhexamethylene biguanide 135 3.87 3.77 86.2 hydrochloridezoxamide wettable powder (polyhexamethylene biguanide hydrochloride:zoxamide = 82:3) Example 20% polyhexamethylene biguanide 67.5 3.76 14.06 47.1 11 aqueous solution 30% zoxamide wettable powder 67.5 4.04 16.22 43.2 Anticipated control effect after 70.0 mixing them 60% polyhexamethylene 135 3.93 3.58 87.1 biguanidezoxamide wettable powder (polyhexamethylene biguanide:zoxamide = 30:30) Example 20% polyhexamethylene biguanide 3.6 3.87 25.38 7.2 12 aqueous solution 30% zoxamide wettable powder 131.4 3.87 5.96 78.2 Anticipated control effect after 79.8 mixing them 75% polyhexamethylene biguanide 135 3.94 3.87 86.1 sulfatezoxamide wettable powder (polyhexamethylene biguanide sulfate:zoxamide = 2:73) Example 20% polyhexamethylene biguanide 131.9 3.79 4.85 81.9 13 aqueous solution 25% azoxystrobin water dispersible 3.1 3.82 26.02 3.6 granules Anticipated control effect after 82.6 mixing them 88% polyhexamethylene biguanide 135 3.59 3.10 87.8 hydrochlorideazoxystrobin wettable powder (polyhexamethylene biguanide hydrochloride:Azoxystrobin = 86:2) Example 20% polyhexamethylene biguanide 67.5 3.68 13.99 46.2 14 aqueous solution 25% azoxystrobin water dispersible 67.5 3.85 14.91 45.2 granules Anticipated control effect after 70.5 mixing them 50% polyhexamethylene 135 3.78 3.79 85.8 biguanideazoxystrobin wettable powder (polyhexamethylene biguanide:Azoxystrobin = 25:25) Example 20% polyhexamethylene biguanide 4.4 3.87 25.22 7.8 15 aqueous solution 25% azoxystrobin water dispersible 130.5 3.92 5.51 80.1 granules Anticipated control effect after 81.7 mixing them 62% polyhexamethylene biguanide 135 3.81 3.34 87.6 acetateazoxystrobin wettable powder (polyhexamethylene biguanide acetate:azoxystrobin = 2:60) Example 20% polyhexamethylene biguanide 130.6 3.65 4.62 82.1 22 aqueous solution 30% fenamidone wettable powder 4.4 3.89 26.20 4.7 Anticipated control effect after 82.9 mixing them 62% polyhexamethylene biguanide 135 3.79 3.72 86.1 hydrochloridefenamidone wettable powder (polyhexamethylene biguanide hydrochloride:fenamidone = 60:2) Example 20% polyhexamethylene biguanide 67.5 3.78 14.00 47.6 23 aqueous solution 30% fenamidone wettable powder 67.5 3.85 15.10 44.5 Anticipated control effect after 70.9 mixing them 50% polyhexamethylene biguanide 135 3.91 3.81 86.2 acetatefenamidone wettable powder (polyhexamethylene biguanide acetate:fenamidone = 25:25) Example 20% polyhexamethylene biguanide 4.2 3.86 25.12 7.9 24 aqueous solution 30% fenamidone wettable powder 130.8 3.82 5.37 80.1 Anticipated control effect after 81.7 mixing them 65% polyhexamethylene biguanide 135 3.79 3.96 85.2 hydrochloridefenamidone wettable powder (polyhexamethylene biguanide hydrochloride:fenamidone = 2:63) Example 20% polyhexamethylene biguanide 131.8 3.91 5.44 80.3 31 aqueous solution 15% acibenzolar wettable powder 3.2 3.21 21.57 4.9 Anticipated control effect after 81.3 mixing them 85% polyhexamethylene biguanide 135 3.79 3.80 85.8 hydrochlorideacibenzolar wettable powder (polyhexamethylene biguanide hydrochloride:acibenzolar = 83:2) Example 20% polyhexamethylene biguanide 67.5 3.98 14.60 48.1 32 aqueous solution 15% acibenzolar wettable powder 67.5 4.05 15.31 46.5 Anticipated control effect after 72.2 mixing them 60% polyhexamethylene 135 3.99 3.33 88.2 biguanideacibenzolar wettable powder (polyhexamethylene biguanide:acibenzolar = 30:30) Example 20% polyhexamethylene biguanide 3.5 3.73 24.49 7.1 33 aqueous solution 15% acibenzolar wettable powder 131.5 3.32 4.62 80.3 Anticipated control effect after 81.7 mixing them 78% polyhexamethylene biguanide 135 3.54 3.38 86.5 sulfateacibenzolar wettable powder (polyhexamethylene biguanide sulfate:acibenzolar = 2:76) Example 20% polyhexamethylene biguanide 131.9 4.11 5.43 81.3 34 aqueous solution 22.7% dithianon suspension 3.1 3.87 26.47 3.2 Anticipated control effect after 81.9 mixing them 88% polyhexamethylene biguanide 135 3.99 4.03 85.7 hydrochloridedithianon wettable powder (polyhexamethylene biguanide hydrochloride:dithianon = 86:2) Example 20% polyhexamethylene biguanide 67.5 3.65 13.70 46.9 35 aqueous solution 22.7% dithianon suspension 67.5 3.82 15.63 42.1 Anticipated control effect after 69.3 mixing them 50% polyhexamethylene 135 3.72 4.02 84.7 biguanidedithianon wettable powder(polyhexamethylene biguanide:dithianon = 25:25) Example 20% polyhexamethylene biguanide 4.2 3.57 23.41 7.2 36 aqueous solution 22.7% dithianon suspension 130.8 3.95 6.00 78.5 Anticipated control effect after 80.0 mixing them 65% polyhexamethylene biguanide 135 4.13 4.26 85.4 acetatedithianon wettable powder (polyhexamethylene biguanide acetate:dithianon = 2:63) Example 20% polyhexamethylene biguanide 131.8 3.69 5.14 80.3 37 aqueous solution 18% pyraclostrobin wettable 3.2 3.75 25.47 3.9 powder Anticipated control effect after 81.1 mixing them 85% polyhexamethylene biguanide 135 4.02 4.20 85.2 hydrochloridepyraclostrobin wettable powder (polyhexamethylene biguanide hydrochloride:pyraclostrobin = 83:2) Example 20% polyhexamethylene biguanide 67.5 0.00 46.4 38 aqueous solution 18% pyraclostrobin wettable 67.5 3.69 14.92 42.8 powder Anticipated control effect after 69.3 mixing them 50% polyhexamethylene 135 3.79 3.27 87.8 biguanidepyraclostrobin wettable powder (polyhexamethylene biguanide:pyraclostrobin = 25:25) Example 20% polyhexamethylene biguanide 3.3 3.83 25.25 6.7 39 aqueous solution 18% pyraclostrobin wettable 131.7 3.93 5.50 80.2 powder Anticipated control effect after 81.5 mixing them 82% polyhexamethylene biguanide 135 3.74 3.94 85.1 stearatepyraclostrobin wettable powder (polyhexamethylene biguanide stearate:pyraclostrobin = 2:80) Example 20% polyhexamethylene biguanide 131.9 3.87 4.95 81.9 40 aqueous solution 30% trifloxystrobin suspension 3.1 4.02 27.42 3.5 Anticipated control effect after 82.5 mixing them 88% polyhexamethylene biguanidetri- 135 3.95 3.88 86.1 floxystrobin wettable powder (polyhexamethylene biguanide:trifloxystrobin = 86:2) Example 20% polyhexamethylene biguanide 67.5 3.68 13.99 46.2 41 aqueous solution 30% trifloxystrobin suspension 67.5 4.06 16.24 43.4 Anticipated control effect after 69.5 mixing them 50% polyhexamethylene biguanide 135 3.82 3.19 88.2 carbonatetrifloxystrobin wettable powder (polyhexamethylene biguanide carbonate:trifloxystrobin = 25:25) Example 20% polyhexamethylene biguanide 3.1 3.65 24.30 5.8 42 aqueous solution 30% trifloxystrobin suspension 131.9 3.72 5.65 78.5 Anticipated control effect after 79.7 mixing them 86% polyhexamethylene biguanide 135 3.95 4.05 85.5 hydrochloridetrifloxystrobin wettable powder(polyhexamethylene biguanide hydrochloride:trifloxystrobin = 2:84) Water 3.87 27.35 control (CK)

(140) The test results (in Table 21) show that the control effect of the combination of polyhexamethylene biguanide or a salt thereof with benthiavalicarb-isopropyl, zoxamide, azoxystrobin, fenamidone, acibenzolar, dithianon, pyraclostrobin, and dimethomorph respectively on downy mildew of grape is significantly improved, suggesting that the combination has an obvious synergistic effect on downy mildew of grape.