Melon variety NUN 16121 MEM

Abstract

The invention relates to the field of Cucumis melo, in particular to a new variety of melon designated NUN 16121 MEM as well as plants, seeds and melon fruits thereof.

Claims

1. A plant, plant part or seed of melon variety NUN 16121 MEM, wherein a representative sample of said seed has been deposited under Accession Number NCIMB 42837.

2. The plant part of claim 1, further defined as a leaf, pollen, an ovule, a fruit, a scion, a rootstock, cutting, flower or a part of any of these or a cell.

3. A seed grown on the plant of claim 1.

4. A Cucumis melo plant, or a part thereof which does not significantly differ at the level of 5%, when grown under the same conditions, from the plant of claim 1 in any of the distinguishing characteristics selected from Tables 1 and 2.

5. A melon plant, or a part thereof which does not significantly differ from the plant of claim 1, when grown under the same conditions.

6. A tissue or cell culture of regenerable cells of the plant of claim 1.

7. The tissue or cell culture according to claim 6, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, parts of seeds, seedcoat, hypocotyl, pollen, leaves, anthers, roots, root tips, pistil, petiole, flower, fruit, seed, stem and stalks.

8. A melon plant regenerated from the tissue or cell culture of claim 6, wherein the plant has all of the physiological and morphological characteristics of the plant of claim 1, where numerical values are determined at the 5% significance level.

9. A method of producing of the plant of claim 1, or a part thereof, comprising vegetative propagation of the plant of claim 1.

10. The method of claim 9, wherein said vegetative propagation comprises regenerating a whole plant from a part of the plant of claim 1.

11. The method of claim 9, wherein said part is a cutting, a cell culture or a tissue culture.

12. A vegetative propagated plant of claim 1, or a part thereof, wherein the plant has all of the physiological and morphological characteristics of the plant of claim 1 when determined at the 5% significance level.

13. A method of producing a melon plant, comprising crossing the plant of claim 1 with a second melon plant one or more times.

14. A melon plant having one physiological and/or morphological characteristic which is different from those of the plant of claim 1 and which otherwise has all the physiological and morphological characteristics of the plant of claim 1 as listed in Table 1, when determined at the 5% significance level and when grown under the same conditions.

15. A food or feed product comprising the plant part of claim 2 wherein the plant part can be identified as a part of the plant of the invention.

16. A melon plant comprising at least a first set of the chromosomes of the plant of claim 1.

17. The plant of claim 1 further comprising a single locus conversion, wherein said plant has all or all but one of the morphological and physiological characteristics of the plant of claim 1 when grown under the same conditions, optionally wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

18. A plant comprising the scion or rootstock of melon variety NUN 16121 MEM, wherein a representative sample of seed of said variety has been deposited under Accession Number NCIMB 42837.

19. A method of making doubled haploids of the plant of claim 1 comprising the step of making double haploid cells from haploid cells from the plant of claim 1 or a seed of claim 1.

Description

DETAILED DESCRIPTION

(1) The present invention relates to a Cucumis melo variety, referred to as NUN 16121 MEM, which differs from the most similar comparison variety Caribbean Gold in one or more characteristics (referred herein to as distinguishing characteristics or distinguishing morphological and/or physiological characteristics (or essential physiological and/or morphological characteristics) selected from: 1) lower average fruit weight at edible maturity; 2) lower average fruit length at edible maturity; 3) flesh flavor at edible maturity that is type 3: very spicy instead of type 1: mild of Caribbean Gold; 4) flesh aroma at edible maturity that is type 2: faint instead of type 1: absent of Caribbean Gold; 5) higher rind thickness at medial; 6) lower seed cavity length; 7) earlier maturity in days from seeding to harvest; 8) higher leaf length (mature blade of third leaf); 9) higher leaf width (mature blade of third leaf); and 10) fruit shape that is type 3: round instead of type 2: oval of Caribbean Gold. Also encompassed by the present invention are progeny plants having all but 1, 2, or 3 of the morphological and/physiological characteristics of NUN 16121 MEM and methods of producing plants in accordance with the present invention.

(2) A melon plant of NUN 16121 MEM differs from the most similar comparison variety Caribbean Gold in one or more characteristics (referred herein to as distinguishing characteristics or distinguishing morphological and/or physiological characteristics (or essential physiological and/or morphological characteristics) selected from: 1) average fruit weight at edible maturity; 2) average fruit length at edible maturity; 3) flesh flavor at edible maturity; 4) Flesh aroma at edible maturity; 5) rind thickness at medial; 6) seed cavity length; 7) maturity in days from seeding to harvest; 8) leaf length; 9) leaf width; and 10) fruit shape.

(3) In another embodiment the plant of the invention is resistant to some pests and diseases: NUN 16121 MEM has high resistance to Fusarium oxysporum f. sp. melonis race 0, race 1 and race 2 (9 on a scale of 1-9) as well as moderate resistance to Spaerotheca fuliginea (Podospaera xanthii) (Powdery mildew) race 1, race 2 and race 5 (2 on a scale of 1-3).

(4) In yet another aspect, said melon variety NUN 16121 MEM may further exhibit at least one further trait, different from the most similar comparison variety Caribbean Gold, selected from the group consisting of a) average leaf (mature blade of third leaf) petiole diameter; b) average blossom scar diameter of fruit at edible maturity; and c) average peduncle diameter of fruit at edible maturity.

(5) It is understood that significant differences refer to statistically significant differences, when comparing the characteristic between two plant lines or varieties when grown under the same conditions. Preferably at least about 10, 15, 20 or more plants per line or variety are grown under the same conditions (i.e. side by side) and characteristics are measured on at least about 10, 15, 20 or more randomly selected plant or plant parts to obtain averages. Thus, physiological and morphological characteristics or traits are commonly evaluated at a significance level of 1%, 5% or 10%, when measured in plants grown under the same environmental conditions. Alternatively, significance or statistical significance of differences can be expressed as a p-value. A p-value represents the probability of obtaining a result equal to or more extreme than the result actually observed. ANOVA is a suitable method for determining the value of p (Clewer, A. G., and D. H. Scarisbrick. 2001). Thus, physiological and morphological characteristics or traits are commonly evaluated at a significance level of p0.1, or preferably p0.05 or even more preferably p0.01 when measured in plants grown under the same environmental condition.

(6) Thus, in one aspect, the invention provides seeds of the melon variety designated NUN 16121 MEM wherein a representative sample of seeds of said variety was deposited under the Budapest Treaty, with Accession number NCIMB 42837.

(7) Seeds of NUN 16121 MEM are obtainable by crossing the male parent with the female parent and harvesting the seeds produced on the female parent. The resultant NUN 16121 MEM seeds can be grown to produce NUN 16121 MEM plants. In one embodiment a plurality of NUN 16121 MEM seeds are packaged into small and/or large containers (e.g., bags, cartons, cans, etc.). The seeds may be disinfected, primed and/or treated with various compounds, such as seed coatings or crop protection compounds.

(8) Also provided are plants of melon variety NUN 16121 MEM, or a fruit or other plant part thereof, produced from seeds, wherein a representative sample of said seeds has been deposited under the Budapest Treaty, with Accession Number NCIMB 42837. Also included is a cell culture or tissue culture produced from such a plant. It is understood that such tissue or cell culture comprising cells or protoplasts from the plant of the invention can be obtained from a plant part selected from the group consisting of embryos, meristems, cotyledons, hypocotyl, pollen, leaves, anthers, roots, root tips, pistil, petiole, flower, fruit, seed, parts of seeds, seedcoat, stem and stalks. In one embodiment a plant regenerated from such a cell or tissue culture said plant expressing all the morphological and physiological characteristics of NUN 16121 MEM.

(9) In one embodiment the invention provides a melon plant regenerated from the tissue or cell culture of NUN 16121 MEM, wherein the plant has all of the physiological and morphological characteristics of NUN 16121 MEM as listed in Table 1 and/or 2 when determined at the 5% significance level. In another embodiment, the invention provides a melon plant regenerated from the tissue or cell culture of NUN 16121 MEM, wherein the plant has all of the physiological and morphological characteristics of NUN 16121 MEM when determined at the 5% significance level.

(10) Plants of NUN 16121 MEM can be produced by seeding directly in the ground (e.g., field) or by germinating the seeds in controlled environment conditions (e.g., greenhouses) and then transplanting the seedlings into the field. For example, the seed can be sown into prepared seed beds where they will remain for the entire production of the crop. Alternatively, the melon seed may be planted or transplanted in prepared mounds.

(11) In another aspect, the invention provides for a melon plant of variety NUN 16121 MEM, a representative sample of seed from said variety has been deposited under the Budapest Treaty, with Accession number NCIMB 42837.

(12) In other aspects, the invention provides for a fruit or parts thereof of melon variety NUN 16121 MEM, or a plant part, such as pollen, flowers, shoots or cuttings of variety NUN 16121 MEM or parts thereof.

(13) In one embodiment a plant of the invention comprises at least 3, 4, 5 or more, e.g. 6, 7, 8, 9 or all of the following morphological and/or physiological characteristics (i.e. distinguishing characteristics (average values; measured at harvest or market maturity, as indicated on the USDA Objective description of varietyMelon and Table 1 and/or 2 (unless indicated otherwise), when grown under the same environmental conditions): 1) average fruit weight at edible maturity; 2) average fruit length at edible maturity; 3) flesh flavor at edible maturity; 4) Flesh aroma at edible maturity; 5) rind thickness at medial; 6) seed cavity length; 7) maturity in days from seeding to harvest; 8) leaf length; 9) leaf width; and 10) fruit shape.

(14) In still another aspect the invention provides a method of producing a melon plant, comprising crossing a plant of melon variety NUN 16121 MEM with a second melon plant one or more times, and selecting progeny from said crossing.

(15) In yet another aspect the invention provides a method of producing a melon plant, comprising selfing a plant of melon variety NUN 16121 MEM one or more times, and selecting progeny from said selfing.

(16) In other aspects, the invention provides for progeny of variety NUN 16121 MEM such as progeny obtained by further breeding NUN 16121 MEM. Further breeding NUN 16121 MEM includes selfing NUN 16121 MEM one or more times and/or cross-pollinating NUN 16121 MEM with another melon plant or variety one or more times. In particular, the invention provides for progeny that retain all the essential morphological and physiological characteristics of NUN 16121 MEM or that retain one or more (e.g. 1) to 5) or 1) to 10) or all) of the distinguishing characteristics of the melon type described further above, or, in another embodiment, progeny that retain all morphological and physiological characteristics of NUN 16121 MEM as listed in Table 1 and/or 2; when grown under the same environmental conditions, when determined at the 5% significance level. In another aspect, the invention provides for vegetative reproductions of the variety and plants having all but 1, 2, or 3 of the physiological and morphological characteristics of NUN 16121 MEM (e.g. as listed in Table 1 and/or 2).

(17) The morphological and/or physiological differences between plants according to the invention, i.e. NUN 16121 MEM or progeny thereof, or plants having all but 1, 2, or 3 of the physiological and morphological characteristics of NUN 16121 MEM (as listed in Table 1 and/or 2); and other known varieties can easily be established by growing NUN 16121 MEM next to the other varieties (in the same field, under the same environmental conditions), preferably in several locations which are suitable for said melon cultivation, and measuring morphological and/or physiological characteristics of a number of plants (e.g., to calculate an average value and to determine the variation range/uniformity within the variety). For example, trials can be carried out in Acampo Calif., USA (N 38 degrees 07261/W 121 degrees 18 807, USA, whereby maturity, ploidy, plant sex form, leaf shape, leaf color, stem shape, surface and length, flower size and color, fruit group, mature fruit color, fruit size, fruit shape, rind texture and thickness, flesh texture and color, disease resistance, insect resistance, can be measured and directly compared for species of Cucumis melo.

(18) The morphological and physiological characteristics (and distinguishing characteristics) of NUN 16121 MEM, are provided in the Examples, in Table 1 and/or 2. Encompassed herein are also plants derivable from NUN 16121 MEM (e.g. by selfings and/or crossing and/or backcrossing with NUN 16121 MEM and/or progeny thereof) comprising all the physiological and morphological characteristics of NUN 16121 MEM listed in Table 1 and/or 2 as determined at the 5% significance level for numerical characteristics or identical for non-numerical characteristics when grown under the same environmental conditions and/or comprising one or more (or all; or all except one, two or three) of the distinguishing characteristics as determined at the 5% significance level when grown under the same environmental conditions.

(19) Also at-harvest and/or post-harvest characteristics of fruits can be compared, such as cold storage holding quality, post-harvest rind firmness and/or flesh firmness can be measured using known methods.

(20) (Fruit) Flesh firmness can for example be measured using a penetrometer, e.g. by inserting a probe into the fruit flesh and determining the insertion force, or by other methods. Fruit flesh firmness can for example be measured using a FT 327 Penetrometer, available from QA Supplies LLC, 1185 Pineridge Road, Norfolk, Va. 23502. For melons, it is preferably combined with a 8 mm round tip, which is also known as a 5/16 tip, available from QA Supplies under #2006061-8. It is understood that fruit flesh firmness expressed in kilogram (kg) can be converted into kg/cm. As in this case a tip with a diameter of 8.0 mm was used, the firmness value in kg should be divided by 0.5 (tip surface being 0.5 cm.sup.2). Likewise the kg/cm value can be multiplied by 9.80665 to convert it to N/cm.sup.2 (i.e. kilogram to Newton conversion).

(21) The morphological and/or physiological characteristics may vary somewhat with variation in the environment (such as temperature, light intensity, day length, humidity, soil, fertilizer use), which is why a comparison under the same environmental conditions is preferred. Colors can best be measured against The Munsell Book of Color (Munsell Color Macbeth Division of Kollmorgan Instruments Corporation) or using the Royal Horticultural Society Chart (World wide web at rhs.org.uk/Plants/RHS-Publications/RHS-colour-charts).

(22) In a preferred embodiment, the invention provides for melon fruits of variety NUN 16121 MEM, or a part of the fruit. In another embodiment, the invention provides for a container comprising or consisting of a plurality of harvested melon fruits of NUN 16121 MEM, or progeny thereof, or a derived variety.

(23) In yet a further embodiment, the invention provides for a method of producing a new melon plant. The method comprises crossing a plant of the invention NUN 16121 MEM, or a plant comprising all but 1, 2, or 3 of the morphological and physiological characteristics of NUN 16121 MEM (as listed in Table 1 and/or 2), or a progeny plant thereof, either as male or as female parent, with a second melon plant (or a wild relative of melon) one or more times, and/or selfing a melon plant according to the invention i.e. NUN 16121 MEM, or a progeny plant thereof, one or more times, and selecting progeny from said crossing and/or selfing. The second melon plant may for example be a line or variety of the species Cucumis melo, or other Cucumis species or even other Cucurbitacea species.

(24) Progeny are a later generation (of seeds) produced from the first cross of the F1 hybrid with another plant (F2) or with itself (S2), or any further generation produced by crossing and/or selfing (F3, F4, etc.) and/or backcrossing (BC2, BC3, etc.) one or more selected plants of the F2 and/or S2 and/or BC2 generation (or plants of any further generation, e.g. the F3) with another melon plant (and/or with a wild relative of melon). Progeny may have all the physiological and morphological characteristics of melon variety NUN 16121 MEM when grown under the same environmental conditions and/or progeny may have (be selected for having) one or more of the distinguishing characteristics of melon of the invention. Using common breeding methods such as backcrossing or recurrent selection, one or more specific characteristics may be introduced into NUN 16121 MEM, to provide or a plant comprising all but 1, 2, or 3 or more of the morphological and physiological characteristics of NUN 16121 MEM (as listed in Table 1 and/or 2).

(25) The invention provides for methods of producing plants which retain all the morphological and physiological characteristics of NUN 16121 MEM. The invention provides also for methods of producing a plant comprising all but 1, 2, or 3 or more of the morphological and physiological characteristics of NUN 16121 MEM (e.g. as listed in Table 1 and/or 2), but which are still genetically closely related to NUN 16121 MEM. The relatedness can, for example be determined by fingerprinting techniques (e.g., making use of isozyme markers and/or molecular markers such as SNP markers, AFLP markers, microsatellites, minisatellites, RAPD markers, RFLP markers and others). A plant is closely related to NUN 16121 MEM if its DNA fingerprint is at least 80%, 90%, 95% or 98% identical to the fingerprint of NUN 16121 MEM. In a preferred embodiment AFLP markers are used for DNA fingerprinting (Vos et al. 1995, Nucleic Acid Research 23: 4407-4414). A closely related plant may have a Jaccard's Similarity index of at least about 0.8, preferably at least about 0.9, 0.95, 0.98 or more (Parvathaneni et al., J. Crop Sci. Biotech. 2011 (March) 14 (1): 3943).

(26) The invention also provides plants and varieties obtained by these methods. Plants may be produced by crossing and/or selfing, or alternatively, a plant may simply be identified and selected amongst NUN 16121 MEM plants, or progeny thereof, e.g. by identifying a variant within NUN 16121 MEM or progeny thereof (e.g. produced by selfing) which variant differs from NUN 16121 MEM in one, two or three of the morphological and/or physiological characteristics (e.g. in one, two or three distinguishing characteristics), e.g. those listed in Table 1 and/or 2 or others. In one embodiment the invention provides a melon plant having a Jaccard's Similarity index with NUN 16121 MEM of at least 0.8, e.g. at least 0.85, 0.9, 0.95, 0.98 or even at least 0.99.

(27) The present invention also provides melon seeds and plants produced by a process that comprises crossing a first parent melon plant with a second parent melon plant, wherein at least one of the first or second parent melon plants is a plant provided herein, such as from variety NUN 16121 MEM. In another embodiment of the invention, melon seed and plants produced by the process are first filial generation (F1) melon seed and plants produced by crossing a plant in accordance with the invention with another, distinct plant.

(28) The present invention further contemplates plant parts of such an F1 melon plant, and methods of use thereof. Therefore, certain exemplary embodiments of the invention provide an F1 melon plant and seed thereof.

(29) WO2013182646 which is incorporated by reference, relates to a non-destructive method for analyzing maternal DNA of a seed. In this method the DNA is dislodged from the seed coat surface and can be used to collect information on the genome of the maternal parent of the seed. This method for analyzing maternal DNA of a seed, comprises the steps of contacting a seed with a fluid to dislodge DNA from the seed coat surface, and analyzing the DNA thus dislodged from the seed coat surface using methods known in the art. The skilled person is thus able to determine whether a seed has grown on a plant of NUN 16121 MEM (i.e. is progeny of NUN 16121 MEM), because the seed coat is genetically identical to NUN 16121 MEM. In one embodiment, the present invention relates to a seed coat comprising maternal tissue of NUN 16121 MEM In another embodiment the invention relates to a melon seed comprising a seed coat that comprises maternal tissue from NUN 16121 MEM.

(30) By crossing and/or selfing also (one or more) single traits may be introduced into the variety of the invention i.e. NUN 16121 MEM (e.g., using backcrossing breeding schemes), while retaining the remaining morphological and physiological characteristics of NUN 16121 MEM and/or while retaining one or more distinguishing characteristics. A single trait converted plant may thereby be produced. For example, disease resistance genes may be introduced, genes responsible for one or more quality traits, yield, etc. Both single genes (dominant or recessive) and one or more QTLs (quantitative trait loci) may be transferred into NUN 16121 MEM by breeding with NUN 16121 MEM.

(31) Any pest or disease resistance genes may be introduced into a plant according to the invention, i.e. NUN 16121 MEM, progeny thereof or into a plant comprising all but 1, 2, or 3 or more of the morphological and physiological characteristics of NUN 16121 MEM (e.g. as listed in Table 1 and/or 2). Resistance to one or more of the following diseases or pests is preferably introduced into plants of the invention: Bacterial Wilt, Root Rot, Crown Blight, Melon Rust, Powdery Mildew, Verticillum Wilt, Sulphur Burn, Scab, Watermelon Mosaic, Downy Mildew, Fusarium oxysporum f. sp. melonis (Fom) race 0, Fusarium oxysporum f. sp. melonis (Fom) race 1, Fusarium oxysporum f. sp. melonis (Fom) race 2, Fusarium oxysporum f. sp. melonis (Fom) race 1-2, Fusarium Wilt R2, Anthracnose, Cucumber Mosaic, Squash Mosaic, Root Knot (Nematode), Aphid, Pickle Worm, Darkling Ground Beetle, Banded Cucumber Beetle, Mite, Western Spotted Cucumber Beetle, Melon Leafhopper, Melon Worm, Western Striped Cucumber Beetle and Melon Leafminer. Other resistance genes, against pathogenic viruses, fungi, bacteria, nematodes, insects or other pests may also be introduced.

(32) Thus, invention also provides a method for developing a melon plant in a melon breeding program, using a melon plant of the invention, or its parts as a source of plant breeding material. Suitable plant breeding techniques are recurrent selection, backcrossing, pedigree breeding, mass selection, mutation breeding and/or genetic marker enhanced selection. For example, in one aspect, the method comprises crossing NUN 16121 MEM or progeny thereof, or a plant comprising all but 1, 2, or 3 or more of the morphological and physiological characteristics of NUN 16121 MEM (e.g. as listed in Table 1 and/or 2), with a different melon plant, and wherein one or more offspring of the crossing are subject to one or more plant breeding techniques selected from the group consisting of recurrent selection, backcrossing, pedigree breeding, mass selection, mutation breeding and genetic marker enhanced selection (see e.g. Brotman et al., Theor Appl Genet (2002) 104:1055-1063). For breeding methods in general see Principles of Plant Genetics and Breeding, 2007, George Acquaah, Blackwell Publishing, ISBN-13: 978-1-4051-3646-4.

(33) The invention thus also provides a method of introducing a single locus conversion, or single trait conversion or introducing a desired trait, into a melon plant according to the invention and/or into NUN 16121 MEM comprising: (a) crossing a melon plant of variety NUN 16121 MEM, a representative sample of seed of said variety having been deposited under Accession Number NCIMB 42837, with a second melon plant comprising a desired single locus to produce F1 progeny plants; (b) selecting F1 progeny plants that have the single locus; (c) crossing the selected progeny plants with a plant of NUN 16121 MEM, to produce backcross progeny plants; (d) selecting backcross progeny plants that have the single locus and one or more (or all) distinguishing characteristics of melon according to the invention and/or all the physiological and morphological characteristics of NUN 16121 MEM to produce selected backcross progeny plants; and (e) optionally repeating steps (c) and (d) one or more times in succession to produce selected second, third or fourth or higher backcross progeny plants that comprise the single locus and otherwise one or more (or all) the distinguishing characteristics of the melons according to the invention and/or comprise all of the physiological and morphological characteristics of NUN 16121 MEM, when grown in the same environmental conditions. The invention further relates to plants obtained by this method.

(34) The above method is provided, wherein the single locus confers a trait, wherein the trait is pest resistance or disease resistance.

(35) In one embodiment the trait is disease resistance and the resistance is conferred to Bacterial Wilt, Root Rot, Crown Blight, Melon Rust, Powdery Mildew, Verticillum Wilt, Sulphur Burn, Scab, Watermelon Mosaic, Downy Mildew, Fusarium oxysporum f. sp. melonis (Fom) race 0, Fusarium oxysporum f. sp. melonis (Fom) race 1, Fusarium oxysporum f. sp. melonis (Fom) race 2, Fusarium oxysporum f. sp. melonis (Fom) race 1-2, Fusarium Wilt R2, Anthracnose, Cucumber Mosaic, Squash Mosaic, Root Knot (Nematode), Aphid, Pickle Worm, Darkling Ground Beetle, Banded Cucumber Beetle, Mite, Western Spotted Cucumber Beetle, Melon Leafhopper, Melon Worm, Western Striped Cucumber Beetle or Melon Leafminer.

(36) The invention also provides a melon plant comprising at least a first set of the chromosomes of melon variety NUN 16121 MEM, a sample of seed of said variety having been deposited under Accession Number NCIMB 42837; optionally further comprising a single locus conversion, wherein said plant has essentially all of the morphological and physiological characteristics of the plant comprising at least a first set of the chromosomes of melon NUN 16121 MEM. In another embodiment, this single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

(37) In one embodiment, NUN 16121 MEM may also be mutated (by e.g. irradiation, chemical mutagenesis, heat treatment, etc.) and mutated seeds or plants may be selected in order to change one or more characteristics of NUN 16121 MEM. Methods such as TILLING may be applied to melon populations in order to identify mutants. Similarly, NUN 16121 MEM may be transformed and regenerated, whereby one or more chimeric genes are introduced into the variety or into a plant comprising all but 1, 2, 3, or more of the morphological and physiological characteristics (e.g. as listed in Table 1 and/or 2). Transformation can be carried out using standard methods, such as Agrobacterium tumefaciens mediated transformation or biolistics, followed by selection of the transformed cells and regeneration into plants. A desired trait (e.g. genes conferring pest or disease resistance, herbicide, fungicide or insecticide tolerance, etc.) can be introduced into NUN 16121 MEM, or progeny thereof, by transforming NUN 16121 MEM or progeny thereof with a transgene that confers the desired trait, wherein the transformed plant retains all the phenotypic and/or morphological and/or physiological characteristics of NUN 16121 MEM or the progeny thereof and contains the desired trait.

(38) The invention also provides for progeny of hybrid (F1) variety NUN 16121 MEM obtained by further breeding with NUN 16121 MEM. In one aspect progeny are F2 progeny obtained by crossing NUN 16121 MEM with another plant or S2 progeny obtained by selfing NUN 16121 MEM. Also encompassed are F3 progeny obtained by selfing the F2 plants. Further breeding encompasses traditional breeding (e.g., selfing, crossing, backcrossing), marker assisted breeding, and/or mutation breeding. In one embodiment, the progeny have one or more (or all) of the distinguishing characteristics mentioned further above when grown under the same environmental conditions. In a further embodiment the progeny have all the physiological and morphological characteristics of variety NUN 16121 MEM when grown under the same environmental conditions. In another embodiment the progeny have one, two, or three distinct traits (qualitative or quantitative) introduced into NUN 16121 MEM, while retaining all the other physiological and morphological characteristics of variety NUN 16121 MEM when grown under the same environmental conditions.

(39) The invention also provides a plant having one, two or three physiological and/or morphological characteristics which are different from those of NUN 16121 MEM and which otherwise has all the physiological and morphological characteristics of NUN 16121 MEM, wherein a representative sample of seed of variety NUN 16121 MEM has been deposited under Accession Number NCIMB 42837. In particular plants which differ from NUN 16121 MEM in none, one, two or three of the characteristics mentioned in Table 1 and/or 2 are encompassed.

(40) In one aspect, the plant having one, two or three physiological and/or morphological characteristics which are different from those of NUN 16121 MEM and which otherwise has all the physiological and morphological characteristics of NUN 16121 MEM differs from NUN 16121 MEM in one, two or three of the distinguishing morphological and/or physiological characteristics selected from 1) average fruit weight at edible maturity; 2) average fruit length at edible maturity; 3) flesh flavor at edible maturity; 4) Flesh aroma at edible maturity; 5) rind thickness at medial; 6) seed cavity length; 7) maturity in days from seeding to harvest; 8) leaf length; 9) leaf width; and 10) fruit shape.

(41) In another embodiment the plant having one, two or three physiological and/or morphological characteristics which are different from those of NUN 16121 MEM and which otherwise has all the physiological and morphological characteristics of NUN 16121 MEM differs from NUN 16121 MEM in one, two or three morphological or physiological characteristic other than the distinguishing morphological and/or physiological characteristics (or essential physiological and/or morphological characteristics) of NUN 16121 MEM selected from: 1) average fruit weight at edible maturity; 2) average fruit length at edible maturity; 3) flesh flavor at edible maturity; 4) Flesh aroma at edible maturity; 5) rind thickness at medial; 6) seed cavity length; 7) maturity in days from seeding to harvest; 8) leaf length; 9) leaf width; and 10) fruit shape.

(42) Melons according to the invention, such as the variety NUN 16121 MEM, or its progeny, or a plant having all physiological and/or morphological characteristics but one, two or three which are different from those of NUN 16121 MEM, can also be reproduced using vegetative reproduction methods. Therefore, the invention provides for a method of producing plants, or a part thereof, of variety NUN 16121 MEM, comprising vegetative propagation of variety NUN 16121 MEM. Vegetative propagation comprises regenerating a whole plant from a plant part of variety NUN 16121 MEM (or from its progeny or from or a plant having all physiological and/or morphological characteristics but one, two or three, which are different from those of NUN 16121 MEM), such as a cutting, a cell culture or a tissue culture.

(43) The invention also concerns methods of vegetatively propagating a plant of the invention. In certain embodiments, the method comprises the steps of: (a) collecting tissue or cells capable of being propagated from a plant of the invention; (b) cultivating said tissue or cells to obtain proliferated shoots; and (c) rooting said proliferated shoots, to obtain rooted plantlets. Steps (b) and (c) may also be reversed, i.e. first cultivating said tissue to obtain roots and then cultivating the tissue to obtain shoots, thereby obtaining rooted plantlets. The rooted plantlets may then be further grown, to obtain plants. In one embodiment, the method further comprises step (d) growing plants from said rooted plantlets

(44) The invention also provides for a vegetatively propagated plant of variety NUN 16121 MEM (or from its progeny or from or a plant having all but one, two or three physiological and/or morphological characteristics which are different from those of NUN 16121 MEM, or a part thereof, having one or more distinguishing characteristics and/or all the morphological and physiological characteristics of NUN 16121 MEM (except for the characteristics differing), when grown under the same environmental conditions.

(45) Parts of NUN 16121 MEM (or of its progeny or of a plant having all physiological and/or morphological characteristics but one, two or three which are different from those of NUN 16121 MEM) encompass any cells, tissues, organs obtainable from the seedlings or plants, such as but not limited to: melon fruits or parts thereof, cuttings, hypocotyl, cotyledon, pollen, scion and the like. Such parts can be stored and/or processed further. Encompassed are therefore also food or feed products comprising one or more of such parts, such as canned, chopped, cooked, roasted, preserved, frozen, dried, pickled, or juiced melon fruit from NUN 16121 MEM or from progeny thereof, or from a derived variety, such as a plant having all but one, two or three physiological and/or morphological characteristics which are different from those of NUN 16121 MEM.

(46) In one aspect haploid plants and/or double haploid plants of NUN 16121 MEM, or a plant having all but one, two or three physiological and/or morphological characteristics which are different from those of NUN 16121 MEM, or progeny of any of these, are encompassed herein. Haploid and double haploid (DH) plants can, for example, be produced by cell or tissue culture and chromosome doubling agents and regeneration into a whole plant. For DH production chromosome doubling may be induced using known methods, such as colchicine treatment or the like.

(47) Also provided are plant parts derived from variety NUN 16121 MEM (or from its progeny or from a plant having all but one, two or three physiological and/or morphological characteristics which are different from those of NUN 16121 MEM), or from a vegetatively propagated plant of NUN 16121 MEM (or from its progeny or from a plant having all but one, two or three physiological and/or morphological characteristics which are different from those of NUN 16121 MEM), being selected from the group consisting of: harvested fruits or parts thereof, pollen, cells, leaves or parts thereof, petioles, cotyledons, hypocotyls, shoots or parts thereof, stems or parts thereof, or vines or parts thereof, roots or parts thereof, cuttings, or flowers.

(48) In one embodiment, the invention provides for extracts of a plant described herein and compositions comprising or consisting of such extracts. In a preferred embodiment, the extract consists of or comprises tissue of a plant described herein or is obtained from such tissue.

(49) In still yet another aspect, the invention provides a method of determining the genotype of a plant of the invention comprising detecting in the genome (e.g., a sample of nucleic acids) of the plant at least a first polymorphism. The method may, in certain embodiments, comprise detecting a plurality of polymorphisms in the genome of the plant, for example by obtaining a sample of nucleic acid from a plant and detecting in said nucleic acids a plurality of polymorphisms. The method may further comprise storing the results of the step of detecting the plurality of polymorphisms on a computer readable medium

(50) The invention also provides for a food or feed product comprising or consisting of a plant part described herein wherein the plant part can be identified as a part of the plant of the invention. Preferably, the plant part is a melon fruit or part thereof and/or an extract from a fruit or another plant part described herein. The food or feed product may be fresh or processed, e.g., dried, grinded, powdered, pickled, chopped, cooked, juiced, preserved, pickled, or powdered canned, steamed, boiled, blanched and/or frozen, etc.

(51) In a preferred embodiment, the present invention and/or embodiments thereof relate to food and o/or a food product (or a feed) comprising a part of the melon plant of the invention NUN 16121 MEM (e.g. a bulb or a part thereof or a seed) wherein the genotype of the plant of the invention is present so that the plant or plant part of the invention can still be identified. A plant part can for example be identified by isolating DNA of the plant part and comparing the DNA sequence with that of a plant of NUN 16121 MEM (e.g. by alignment, if at least 99% of the DNA is identical (e.g. 99.5, 99.8 or even 99.9%) then the skilled person will recognize the plant part as a part of NUN 16121 MEM). The skilled person will know how to apply DNA sequence alignment techniques that are known in the art. Alternatively, he may use a set of SNP markers that are unique for NUN 16121 MEM to identify plant parts as part NUN 16121 MEM.

(52) For example, containers such as cans, boxes, crates, bags, cartons, Modified Atmosphere Packagings, films (e.g. biodegradable films), etc. comprising plant parts of plants (fresh and/or processed) described herein are also provided herein.

(53) Marketable melon fruits are generally sorted by size and quality after harvest. Alternatively the melon fruits can be sorted by Brix or sugar content.

(54) Melons may also be grown for use in grafting or inosculation as rootstocks (stocks) or scions (scions). Typically, different types of melons are grafted to enhance disease resistance, which is usually conferred by the rootstock, while retaining the horticultural qualities usually conferred by the scion. It is not uncommon for grafting to occur between cultivated melon varieties and related Cucurbitae species. Methods of grafting and vegetative propagation are well-known in the art.

(55) So in one aspect the invention relates to a plant comprising a rootstock or scion of NUN 16121 MEM.

(56) Using methods known in the art like reverse breeding, it is possible to produce parental lines for a hybrid plant such as NUN 16121 MEM; where normally the hybrid is produced from the parental lines. Such methods are based on the segregation of individual alleles in the spores produced by a desired plant and/or in the progeny derived from the self-pollination of that desired plant, and on the subsequent identification of suitable progeny plants in one generation, or in a limited number of inbred cycles. Such a method is known from WO2014076249 or from Wijnker et al, Nature Protocols Volume: 9, Pages: 761-772 (2014) DOI: doi:10.1038/nprot.2014.049, which are enclosed by reference. Such method for producing parental lines for a hybrid organism, comprises the steps of: a) defining a set of genetic markers that are present in a heterozygous form (H) in a partially heterozygous starting organism; b) producing doubled haploid lines from spores of the starting organism: c) genetically characterizing the doubled haploid lines thus obtained for the said set of genetic markers to determine whether they are present in a first homozygous form (A) or in a second homozygous form (B); d) selecting at least one pair of doubled haploid lines that have complementary alleles for at least a subset of the genetic markers, wherein each member of the pair is suitable as a parental line for a hybrid organism.

(57) Thus in one aspect, the invention relates to a method of producing a combination of parental lines of a plant of the invention (NUN 16121 MEM) comprising the step of making double haploid cells from haploid cells from the plant of the invention (NUN 16121 MEM) or a seed of that plant; and optionally crossing these parental lines to produce and collect seeds. In another aspect, the invention relates to a combination of parental lines produced by this method. In still another aspect said combination of parental lines can be used to produce a seed or plant of NUN 16121 MEM when these parental lines are crossed. In still another aspect, the invention relates to a combination of parental lines from which a seed or plant having all but one, two or three physiological and/or morphological characteristics of NUN 16121 MEM can be produced; or in another aspect, wherein a seed or plant having the distinguishing characteristics 1)-5) or 1)-10) of NUN 16121 MEM, as herein defined, can be produced when grown under the same environmental conditions. In still another aspect, the invention relates to a combination of parental lines from which a seed or plant having all the characteristics of NUN 16121 MEM as defined in Table 1 and/or 2 can be produced when grown under the same conditions.

(58) All documents (e.g., patent publications) are herein incorporated by reference in their entirety.

CITED REFERENCES

(59) ams usda.gov/AMSv1.0/getfile?dDocName=STELDEV3003780 On the worldwide web at rhs.org.uk/Plants/RHS-Publications/RHS-colour-charts On the worldwide web at upov.int/edocs/tgdocs/en/tg076.pdf Acquaah, Principles of Plant Genetics and Breeding, 2007, Blackwell Publishing, ISBN-13: 978-1-4051-3646-4 Brotman et al., Theor Appl Genet (2002) 104:1055-1063 DOI 10.1007/s00122-001-0808-x Colijn-Hooymans (1994), Plant Cell, Tissue and Organ Culture 39: 211-217) Ren et al., In Vitro Cell. Dev. Biol.Plant (2013) 49:223-229 DOI 10.1007/s11627-012-9482-8; Parvathaneni et al., J. Crop Sci. Biotech. 2011 (March) 14 (1): 3943 DOI No. 10.1007/s12892-010-0080-1 Vos et al. 1995, Nucleic Acid Research 23: 4407-4414 Wijnker et al, Nature Protocols Volume: 9, Pages: 761-772 (2014) DOI: doi:10.1038/nprot.2014.049 US 2006/0168701 WO2013182646 WO2014076249

EXAMPLES

Development of NUN 16121 MEM

(60) The hybrid NUN 16121 MEM was developed from a male and female proprietary inbred line of Nunhems. The female and male parents were crossed to produce hybrid (F1) seeds of NUN 16121 MEM. The seeds of NUN 16121 MEM can be grown to produce hybrid plants and parts thereof (e.g. melon fruit). The hybrid NUN 16121 MEM can be propagated by seeds or vegetative.

(61) The hybrid variety is uniform and genetically stable. This has been established through evaluation of horticultural characteristics. Several hybrid seed production events resulted in no observable deviation in genetic stability. Coupled with the confirmation of genetic stability of the female and male parents the Applicant concluded that NUN 16121 MEM is uniform and stable.

DEPOSIT INFORMATION

(62) A total of 2500 seeds of the hybrid variety NUN 16121 MEM were deposited according to the Budapest Treaty by Nunhems B.V. on Sep. 29, 2017, at the NCIMB Ltd., Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, United Kingdom (NCIMB). The deposit has been assigned or NCIMB 42837. A deposit of NUN 16121 MEM and of the male and female parent line is also maintained at Nunhems B.V. Access to the deposit will be available during the pendency of this application to persons determined by the Director of the U.S. Patent Office to be entitled thereto upon request. Subject to 37 C.F.R. 1.808(b), all restrictions imposed by the depositor on the availability to the public of the deposited material will be irrevocably removed upon the granting of the patent. The deposit will be maintained for a period of 30 years, or 5 years after the most recent request, or for the enforceable life of the patent whichever is longer, and will be replaced if it ever becomes nonviable during that period. Applicant does not waive any rights granted under this patent on this application or under the Plant Variety Protection Act (7 USC 2321 et seq.).

(63) USDA descriptors are the plant variety descriptors for melon (Cucumis melo)Exhibit C of the U.S. Department of Agriculture, Agricultural Marketing Service, Science and Technology, Plant Variety Protection Office, Beltsville, Md. 20705, which can be downloaded from the world wide web at ams.usda.gov/AMSv1.0/getfile?dDocName=STELDEV3003780 and which is herein incorporated by reference in its entirety.

(64) The most similar variety to NUN 16121 MEM is Caribbean Gold RZ, a commercial variety from RijkZwaan. In Table 1 and 2 a comparison between NUN 16121 MEM and Caribbean Gold RZ is shown based on a trial in the USA. Trial location: Acampo Calif. USA, (coordinates: 38.192873 N, 121.232637 W). Sowing date: Jul. 14, 2015 (sown directly in field).

(65) Two replications of 50 plants each, from which 15 plants or plant parts were randomly selected to measure characteristics. In Table 1 the USDA descriptors of NUN 16121 MEM (this application) and reference Caribbean Gold RZ (commercial variety) are summarized. In Table 2 additional descriptors of NUN 16121 MEM (this application) and reference Caribbean Gold RZ (commercial variety) are summarized.

(66) TABLE-US-00001 TABLE 1 Application Variety Comparison Variety USDA Descriptor NUN 16121 MEM Caribbean Gold RZ 1. TYPE: 5 5 1 = Persian 2 = Honey Dew 3 = Casaba 4 = Crenshaw 5 = Common/Summer 6 = Other 2. AREA OF BEST ADAPTATION IN U.S.A.: 4 4 1 = Southeast 2 = Northeast/North Central 3 = Southwest 4 = Most Areas 3. MATURITY: 75 78 Days From Seeding to Harvest 4. PLANT: Fertility: 1 = Andromonoecious 2 = Monoecious 1 n.r. 3 = Gynoecious 4 = Other Habit: 1 1 1 = Vine 2 = Semi-bush 3 = Bush 5. LEAF: Shape: 1 = Orbicular 2 = Ovate 3 = Reniform 3 3 (Cordate) Lobes: 1 = Not Lobed 2 = Shallowly Lobed 3 = 2 2 Deeply Lobed Color: 1 = Light Green (Honey Dew) 2 = Medium 2 2 Green 3 = Dark Green (Rio Gold) Color Chart Code (RHS chart) Yellow Green 147A Yellow Green 147A Average Length mm 144.5 124.7 Average Width mm 206.7 187.2 Surface: 3 3 1 = Pubescent 2 = Glabrous 3 = Scabrous 6. FRUIT: Average Length in cm 13.3 16.4 Average Diameter in cm 12.9 13.3 Average Weight in gram 1179.3 1620.5 Shape: 1 = Oblate 2 = Oval 3 = Round 4 = 3 2 Elongate-Cylindrical 5 = Spindle 6 = Acorn Surface: 1 = Smooth 2 = Netted 3 = Corrugated 2 2 4 = Warted Blossom Scar: 1 = Obscure 2 = Conspicuous 1 1 Rib Presence: 1 = Absent 2 = Present 1 1 No. Ribs per Fruit N.A. N.A. Rib Width at Medial in mm N.A. N.A. Ribs Surface: 1 = Smooth 2 = Netted N.A. N.A. Suture Depth: 1 = Shallow (Golden Delight) 2 = N.A. N.A. Medium 3 = Deep (Hackensack) Suture Surface: 1 = Smooth 2 = Netted N.A. N.A. Shipping Quality: 1 = Poor (Home Garden) 2 = 3 3 Fair (Short Distance Shipping) 3 = Excellent (Long Distance Shipping) Fruit Abscission: 1 = When Ripe 2 = When 2 2 Overripe 3 = Do Not Abscise 7. RIND NET Net Presence: 3 3 1 = Absent 2 = Sparse 3 = Abundant Distribution: 2 2 1 = Spotty 2 = Covers Entire Fruit Coarseness: 2 2 1 = Fine 2 = Medium Coarse 3 = Very Coarse Interlacing: 1 = None 2 = Some 3 = Complete 3 3 Interstices: 1 = Shallow 2 = Medium Deep 3 = 2 2 Deep 8. RIND TEXTURE: Texture: 1 = Soft 2 = Firm 3 = Hard 2 2 Average Thickness at Medial in mm 2.64 2.32 9. RIND COLOR: Rind Color At Edible Maturity 01-white; 02 = cream; 03 = buff; 04 = yellow; 05 = gold; 06 = green; 07 = orange; 08 = bronze; 09 = brown; 10 = gray; 11 = black; 12 = other. Primary Color/Color Chart Value 06 06 (green 139D) (yellow green 146B) Mottling Color/Color Chart Value N.A. N.A. Net Color/ 03 02 Color Chart Value (162D) (Greyed yellow 162D) Furrow (Suture)/Color Chart Value N.A. N.A. Rind Color At Full Maturity Primary Color/Color Chart Value N.A. N.A. Mottling Color/Color Chart Value N.A. N.A. Net Color/Color Chart Value N.A. N.A. Furrow (Suture)/Color Chart Value N.A. N.A. 10. FLESH (At Edible Maturity): Color Near Cavity/ 05 05 Color Chart Value (24A) (Orange 24A) Color in Center/ 05 05 Color Chart Value (24A) (Orange 24A) Color Near Rind/ 04 04 Color Chart Value (137A) (Green 139A) Refractometer % Soluable Solids (Center of 13.04 12.52 Flesh) Aroma: 1 = Absent 2 = Faint 3 = Strong 2 1 Flavor: 1 = Mild 2 = Somewhat Spicy 3 = Very 3 1 Spicy 11. SEED CAVITY: Average Length in mm 87.03 105.75 Average Width in mm 58.2 57.9 Shape in X-Section: 1 1 1 = Circular 2 = Triangular 12. SEEDS: Average No. Seeds per Fruit 700 509 Average grams per 1,000 Seeds 20 23

(67) TABLE-US-00002 TABLE 2 Application Comparison Variety Variety NUN Caribbean Non-USDA descriptor 16121 MEM Gold RZ Petiole length of third 155.1 146.1 leaf mature blade (mm) Petiole diameter of third 7.46 6.51 leaf mature blade (mm) Peduncle length of fruit 40.0 46.4 at edible maturity (mm) Peduncle diameter of fruit 7.9 5.6 at edible maturity (mm) Blossom scar diameter of fruit 16.9 21.4 at edible maturity (mm) Flesh firmness (measured with 1.22 1.4 8.00 mm tipped penetrometer) (kg)

(68) Table 1 and 2 contain typical values. Values may vary due to environment. Other values that are substantially equivalent are also within the scope of the invention. N.A.=not applicable; n.r.=not recorded.