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INDOLE DERIVATIVE-CONTAINING INHIBITOR, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

20220340547 · 2022-10-27

    Inventors

    Cpc classification

    International classification

    Abstract

    The present invention relates to an indole derivative-containing inhibitor, a preparation method therefor and an application thereof, and in particular, to a compound as represented by general formula (Ia), a preparation method therefor, a pharmaceutical composition comprising the compound, and the use thereof in treating related diseases such as cancer, inflammation, chronic liver disease, diabetes, cardiovascular disease, and AIDS as a kinase inhibitor, especially as a receptor tyrosine kinase inhibitor (TKI), more specifically, as an EGFR or HER2 inhibitor. The compound exhibits good inhibitory activity in EGFR and HER2 20 exon mutations.

    ##STR00001##

    Claims

    1. A compound of formula (Ia), a stereoisomer thereof or a pharmaceutically acceptable salt thereof: ##STR00307## wherein: E is selected from the group consisting of CR.sub.aa and N; ring A is selected from the group consisting of heterocyclyl, aryl and heteroaryl; R.sub.1 is selected from the group consisting of hydrogen, deuterium, alkyl, deuterated alkyl, haloalkyl, alkoxy, haloalkoxy, halogen, amino, nitro, hydroxy, cyano, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl and —(CH.sub.2).sub.nR.sub.aa, wherein the alkyl, haloalkyl, cycloalkyl, heterocyclyl, aryl and heteroaryl are each optionally further substituted; R.sub.2 is selected from the group consisting of hydrogen, deuterium, alkyl, deuterated alkyl, haloalkyl, alkoxy, haloalkoxy, halogen, amino, nitro, hydroxy, cyano, azido, alkoxycarbonyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, —(CH.sub.2).sub.nOR.sub.aa, —(CH.sub.2).sub.nNR.sub.aaR.sub.bb, —NR.sub.aaC(O)R.sub.bb, —C(O)NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —NR.sub.aaC(O)NR.sub.bbR.sub.cc, —NR.sub.aaC(O)NR.sub.bb(CH.sub.2).sub.nR.sub.cc, —C≡CR.sub.aa, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nR.sub.bb, —NR.sub.aaC(O)C≡CR.sub.bb, —C(O)NR.sub.aaR.sub.bb, —C(O)OR.sub.aa, —NR.sub.aaS(O).sub.mR.sub.bb, —O(CH.sub.2).sub.nR.sub.aa, —(CH.sub.2).sub.nP(O)R.sub.aaR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nC(O)R.sub.aa, —NR.sub.aaC(O)OR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mR.sub.aa, —(CH.sub.2).sub.nNR.sub.aaS(O).sub.mR.sub.bb and ##STR00308## wherein the alkyl, haloalkyl, cycloalkyl, heterocyclyl, aryl and heteroaryl are each optionally further substituted; each R.sub.3 is independently selected from the group consisting of hydrogen, deuterium, alkyl, deuterated alkyl, haloalkyl, alkoxy, haloalkoxy, halogen, amino, nitro, hydroxy, cyano, azido, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, —(CH.sub.2).sub.nOR.sub.aa, —(CH.sub.2).sub.nNR.sub.aaR.sub.bb, —NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —NR.sub.aa(CH.sub.2).sub.nNR.sub.bbR.sub.cc, —NR.sub.aaC(O)R.sub.bb, —NR.sub.aaC(O)NR.sub.bbR.sub.cc, —NR.sub.aaC(O)NR.sub.bb(CH.sub.2).sub.nR.sub.cc, —NR.sub.aaC(O)C≡CR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2)NR.sub.bbR.sub.cc, —C(O)NR.sub.aaR.sub.bb, —C(O)OR.sub.aa, —NR.sub.aaS(O).sub.mR.sub.bb, —O(CH.sub.2).sub.nR.sub.aa, —O(CH.sub.2).sub.nNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nP(O)R.sub.aaR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nC(O)R.sub.aa, —NR.sub.aaC(O)OR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mR.sub.aa and —(CH.sub.2).sub.nNR.sub.aaS(O).sub.mR.sub.bb, wherein the alkyl, haloalkyl, cycloalkyl, heterocyclyl, aryl and heteroaryl are each optionally further substituted; each R.sup.1 is independently selected from the group consisting of hydrogen, deuterium, alkyl, deuterated alkyl, haloalkyl, alkoxy, haloalkoxy, halogen, amino, nitro, hydroxy, cyano, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl and —(CH.sub.2).sub.nR.sub.aa, wherein the alkyl, haloalkyl, cycloalkyl, heterocyclyl, aryl and heteroaryl are each optionally further substituted; or, any two of R.sup.1 are bonded to form a cycloalkyl, heterocyclyl, aryl or heteroaryl, wherein the cycloalkyl, heterocyclyl, aryl and heteroaryl are each optionally further substituted; R.sub.aa, R.sub.bb and R.sub.cc are each independently selected from the group consisting of hydrogen, deuterium, alkyl, deuterated alkyl, haloalkyl, alkoxy, hydroxyalkyl, haloalkoxy, halogen, cyano, nitro, hydroxy, amino, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl and heteroaryl, wherein the alkyl, deuterated alkyl, haloalkyl, alkoxy, hydroxyalkyl, haloalkoxy, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl and heteroaryl are each optionally further substituted; x is an integer from 0 to 4; y is an integer from 0 to 4; m is an integer from 0 to 2; n is an integer from 0 to 4.

    2. (canceled)

    3. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein ring A is selected from the group consisting of C.sub.6-14 aryl, 3 to 12 membered heterocyclyl and 5 to 14 membered heteroaryl.

    4. (canceled)

    5. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.1 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, substituted or unsubstituted C.sub.1-3 alkyl, substituted or unsubstituted C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, substituted or unsubstituted C.sub.3-6 cycloalkyl, substituted or unsubstituted C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl and —(CH.sub.2).sub.nR.sub.aa.

    6. (canceled)

    7. (canceled)

    8. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.2 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, azido, alkoxycarbonyl, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 1 to 6 membered alkoxycarbonyl, substituted or unsubstituted 3 to 12 membered heterocyclyl, substituted or unsubstituted C.sub.6-14 aryl, substituted or unsubstituted 5 to 12 membered heteroaryl, substituted or unsubstituted 5 to 14 membered heteroaryl, —NR.sub.aaC(O)NR.sub.bb(CH.sub.2).sub.nR.sub.cc, —C≡CR.sub.aa, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nR.sub.bb, —C(O)NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —C(O)OR.sub.aa, —O(CH.sub.2).sub.nR.sub.aa, —(CH.sub.2).sub.nP(O)R.sub.aaR.sub.bb, —NR.sub.aaS(O).sub.mR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nC(O)R.sub.aa, —NR.sub.aaC(O)OR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mR.sub.aa, —(CH.sub.2).sub.nNR.sub.aaS(O).sub.mR.sub.bb, and ##STR00309##

    9. (canceled)

    10. (canceled)

    11. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.3 is selected from the group consisting of substituted or unsubstituted 3 to 12 membered heterocyclyl, —O(CH.sub.2).sub.nR.sub.e and —O(CH.sub.2).sub.nNR.sub.eR.sub.f, hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, azido, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —NR.sub.aa(CH.sub.2).sub.nNR.sub.bbR.sub.cc, —NR.sub.aaC(O)C≡CR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nNR.sub.bbR.sub.cc, —C(O)NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —C(O)OR.sub.aa, —O(CH.sub.2).sub.nR.sub.aa, —(CH.sub.2).sub.nP(O)R.sub.aaR.sub.bb, —NR.sub.aaS(O).sub.mR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nC(O)R.sub.aa, —NR.sub.aaC(O)OR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mR.sub.aa and —(CH.sub.2).sub.nNR.sub.aaS(O).sub.mR.sub.bb; R.sub.e and R.sub.f are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, azido, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-6 cycloalkyl and C.sub.3-6 heterocyclyl containing 1 to 2 nitrogen atom; wherein the C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.3-6 cycloalkyl and C.sub.3-6 heterocyclyl containing 1 to 2 nitrogen atom are each optionally substituted by one or more substituents selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-6 cycloalkyl, C.sub.3-6 heterocyclyl and —C(O)CH═CR.sub.gR.sub.h; R.sub.g and R.sub.h are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, azido, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-6 cycloalkyl and C.sub.3-6 heterocyclyl R.sub.aa, R.sub.bb and R.sub.cc are each independently selected from the group consisting of hydrogen atom, deuterium atom, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.1-6 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl, wherein the C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl are each optionally further substituted by one or more of deuterium, halogen, amino, cyano, C.sub.1-4 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 haloalkoxy, C.sub.1-6 haloalkyl, C.sub.2-6 alkenylcarbonyl, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, substituted or unsubstituted 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —O(CH.sub.2).sub.nR.sub.AA and —C(O)CH═CHR.sub.AAR.sub.BB; and R.sub.AA and R.sub.BB are each independently selected from the group consisting of hydrogen atom, deuterium atom, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.1-6 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl, wherein the C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl are each optionally further substituted by deuterium, halogen, amino, cyano, C.sub.1-4 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 haloalkoxy, C.sub.1-6 haloalkyl, C.sub.2-6 alkenylcarbonyl, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl or 5 to 14 membered heteroaryl.

    12. (canceled)

    13. (canceled)

    14. (canceled)

    15. (canceled)

    16. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein R.sup.1 is selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, deuterated C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 hydroxyalkyl, C.sub.1-6 alkoxy, C.sub.1-6 haloalkoxy, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl; or, any two of R.sup.1 are bonded to form a C.sub.3-8 cycloalkyl 3 to 12 membered heterocyclyl C.sub.6-14 aryl or 5 to 14 membered heteroaryl.

    17. (canceled)

    18. (canceled)

    19. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein the formula (Ia) is further shown as formula (IIa): ##STR00310## wherein: M.sub.3 is selected from the group consisting of CR.sub.13 and N; R.sub.4 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, azido, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —NR.sub.aaC(O)R.sub.bb, —NR.sub.aaC(O)NR.sub.bb(CH.sub.2).sub.nR.sub.cc, —NR.sub.aaC(O)C≡CR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nNR.sub.bbR.sub.cc, —C(O)NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —C(O)OR.sub.aa, —O(CH.sub.2).sub.nR.sub.aa, —(CH.sub.2).sub.nP(O)R.sub.aaR.sub.bb, —NR.sub.aaS(O).sub.mR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nC(O)R.sub.aa, —NR.sub.aaC(O)OR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mR.sub.aa and —(CH.sub.2).sub.nNR.sub.aaS(O).sub.mR.sub.bb; R.sub.5 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —O(CH.sub.2).sub.nR.sub.aa, —NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —O(CH.sub.2).sub.nNR.sub.aaR.sub.bb and —NR.sub.aa(CH.sub.2).sub.nNR.sub.bbR.sub.cc, wherein the C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl are each optionally further substituted by one or more substituents selected from the group consisting of hydrogen, deuterium, halogen, hydroxy, thiol, amino, C.sub.1-4 alkyl, C.sub.1-4 haloalkyl, C.sub.1-4 alkoxy, C.sub.1-4 haloalkoxy, C.sub.2-6 alkenylcarbonyl, C.sub.1-4 hydroxyalkyl, C.sub.3-8 cycloalkyl, substituted or unsubstituted 3 to 12 membered heterocyclyl and —(CH.sub.2).sub.nNR.sub.ddR.sub.ee; R.sub.6 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, hydroxy, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 haloalkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl and —O(CH.sub.2).sub.nR.sub.aa; R.sub.13 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, hydroxy, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 haloalkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl; R.sub.dd and R.sub.ee are each independently selected from the group consisting of hydrogen atom, deuterium atom, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.1-6 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl, wherein the C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl are each optionally further substituted by deuterium, halogen, amino, cyano, C.sub.1-4 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 haloalkoxy, C.sub.1-6 haloalkyl, C.sub.2-6 alkenylcarbonyl, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl or 5 to 14 membered heteroaryl.

    20. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein the formula (I) is further shown as formula (II): ##STR00311## wherein: R.sub.4 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, azido, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —NR.sub.aaC(O)NR.sub.bb(CH.sub.2).sub.nR.sub.cc, —NR.sub.aaC(O)C≡CR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nNR.sub.bbR.sub.cc, —C(O)NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —C(O)OR.sub.aa, —O(CH.sub.2).sub.nR.sub.aa, —(CH.sub.2).sub.nP(O)R.sub.aaR.sub.bb, —NR.sub.aaS(O).sub.mR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nC(O)R.sub.aa, —NR.sub.aaC(O)OR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mR.sub.aa and —(CH.sub.2).sub.nNR.sub.aaS(O).sub.mR.sub.bb; R.sub.5 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —O(CH.sub.2).sub.nR.sub.aa, —NR.sub.aa(CH.sub.2).sub.nR.sub.bb and —NR.sub.aa(CH.sub.2).sub.nNR.sub.bbR.sub.cc, wherein the C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl are each optionally further substituted by one or more substituents selected from the group consisting of halogen, hydroxy, thiol, C.sub.1-4 alkyl, C.sub.1-4 haloalkyl, C.sub.1-4 alkoxy, C.sub.1-4 hydroxyalkyl and C.sub.3-8 cycloalkyl; R.sub.6 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 haloalkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl.

    21. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.2 is selected from the group consisting of hydrogen, halogen, cyano, azido, alkoxycarbonyl, C.sub.1-6 alkyl, haloC.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, substituted or unsubstituted 5 to 14 membered heteroaryl, —NR.sub.aC(O)NR.sub.c(CH.sub.2).sub.nR.sub.b, —C≡CR.sub.a, —NR.sub.aC(O)CH═CHR.sub.b, —C(O)NR.sub.a(CH.sub.2).sub.nR.sub.b, —C(O)OR.sub.a, —O(CH.sub.2).sub.nR.sub.a, —(CH.sub.2).sub.nS(O).sub.mR.sub.a, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aR.sub.b and —(CH.sub.2).sub.nP(O)R.sub.aR.sub.b, wherein the heterocyclyl is selected from 5 to 8 membered heteroaryl containing 1 to 2 atoms selected from the group consisting of nitrogen, oxygen and sulfur atom, which is optionally substituted by hydroxy, halogen, C.sub.1-6 alkyl or C.sub.1-6 alkoxy; R.sub.a, R.sub.b and R.sub.c are each independently selected from the group consisting of hydrogen, C.sub.1-6 alkyl, C.sub.3-6 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl, which is optionally further substituted by one or more of halogen, hydroxy, C.sub.1-6 alkyl, C.sub.3-6 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl.

    22.-28. (canceled)

    29. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein the formula (I) is further shown as formula (III): ##STR00312## wherein: M.sub.1 and M.sub.2 are each independently selected from the group consisting of CR.sub.9 and N; R.sub.7 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, azido, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —NR.sub.aaC(O)NR.sub.bb(CH.sub.2).sub.nR.sub.cc, —NR.sub.aaC(O)C≡CR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nNR.sub.bbR.sub.cc, —C(O)NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —C(O)OR.sub.aa, —O(CH.sub.2).sub.nR.sub.aa, —(CH.sub.2).sub.nP(O)R.sub.aaR.sub.bb, —NR.sub.aaS(O).sub.mR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nC(O)R.sub.aa, —NR.sub.aaC(O)OR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mR.sub.aa and —(CH.sub.2).sub.nNR.sub.aaS(O).sub.mR.sub.bb; R.sub.8 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —O(CH.sub.2).sub.nR.sub.aa, —NR.sub.aa(CH.sub.2).sub.nR.sub.bb and —NR.sub.aa(CH.sub.2).sub.nNR.sub.bbR.sub.cc, wherein the C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl and 5 to 14 membered heteroaryl are each optionally further substituted by one or more substituents selected from the group consisting of halogen, hydroxy, thiol, C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl and C.sub.3-8 cycloalkyl; R.sub.9 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, azido, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —NR.sub.aaC(O)NR.sub.bb(CH.sub.2).sub.nR.sub.cc, —NR.sub.aaC(O)C≡CR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nR.sub.bb, —NR.sub.aaC(O)CH═CH(CH.sub.2).sub.nNR.sub.bbR.sub.cc, —C(O)NR.sub.aa(CH.sub.2).sub.nR.sub.bb, —C(O)OR.sub.aa, —O(CH.sub.2).sub.nR.sub.aa, —(CH.sub.2).sub.nP(O)R.sub.aaR.sub.bb, —NR.sub.aaS(O).sub.mR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mNR.sub.aaR.sub.bb, —(CH.sub.2).sub.nC(O)R.sub.aa, —NR.sub.aaC(O)OR.sub.bb, —(CH.sub.2).sub.nS(O).sub.mR.sub.aa and —(CH.sub.2).sub.nNR.sub.aaS(O).sub.mR.sub.bb.

    30. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein the formula (I) is further shown as formula (IV): ##STR00313##

    31. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 20, wherein the formula (I) is further shown as shown formula (V): ##STR00314## wherein: R.sub.10 is selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —C≡CR.sub.aa, —CH═CH(CH.sub.2).sub.nR.sub.aa, —CR.sub.aa═CH(CH.sub.2).sub.nR.sub.bb and —CH═CH(CH.sub.2).sub.nNR.sub.aaR.sub.bb.

    32. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 20, wherein the formula (I) is further shown as formula (VI): ##STR00315## wherein: R.sub.11 and R.sub.12 are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, substituted or unsubstituted C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl, substituted or unsubstituted C.sub.3-8 cycloalkyl, 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl, —(CH.sub.2).sub.nR.sub.aa and —(CH.sub.2).sub.nNR.sub.aaR.sub.bb; or, R.sub.11 and R.sub.12, together with the nitrogen atom to which they are attached, form a 3 to 12 membered heterocyclyl or 5 to 10 membered heteroaryl, wherein the 3 to 12 membered heterocyclyl or 5 to 10 membered heteroaryl is optionally substituted by one or more substituents selected from the group consisting of deuterium, halogen, amino, hydroxy, cyano, nitro, oxo, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, C.sub.2-6 alkynyl, deuterated C.sub.1-6 alkyl, C.sub.1-6 haloalkyl, C.sub.1-6 alkoxy, C.sub.1-6 haloalkoxy, C.sub.1-6 hydroxyalkyl, C.sub.3-12 cycloalkyl, substituted or unsubstituted 3 to 12 membered heterocyclyl, C.sub.6-14 aryl, 5 to 14 membered heteroaryl and —(CH.sub.2).sub.nNR.sub.aaR.sub.bb.

    33. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 19, wherein the compound is further shown as formula (VII): ##STR00316## wherein: R.sub.1 is selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, C.sub.1-6 alkyl, C.sub.3-8 cycloalkyl and 3 to 6 membered heterocyclyl, which is optionally further substituted by one or more substituents selected from the group consisting of deuterium, halogen, amino, nitro, hydroxy, cyano, C.sub.1-6 alkyl, C.sub.3-8 cycloalkyl and 3 to 6 membered heterocyclyl; R.sub.11 and R.sub.12 are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl and —(CH.sub.2).sub.nNR.sub.aaR.sub.bb; R.sub.aa and R.sub.bb are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.1-6 alkoxy and 3 to 6 membered heterocyclyl; n is an integer of 0, 1, 2 or 3; or, R.sub.11 and R.sub.12, together with the nitrogen atom to which they are attached, form a 4 to 12 membered heterocyclyl, which is optionally substituted by one or more substituents selected from the group consisting of halogen, hydroxy, amino, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, amino substituted by C.sub.1-6 alkyl, C.sub.3-12 cycloalkyl and substituted or unsubstituted 3 to 12 membered heterocyclyl; each R.sup.1 is independently selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl and C.sub.3-8 cycloalkyl; y is an integer of 0, 1, 2 or 3.

    34. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 19, wherein the compound is further shown as formula (VIII): ##STR00317## wherein: R.sub.2 is selected from the group consisting of C.sub.6-12 aryl, 4 to 7 membered heterocyclyl and 4 to 7 membered heteroaryl, which is optionally further substituted by one or more substituents selected from the group consisting of hydroxy, amino, halogen, C.sub.1-6 alkyl, C.sub.1-6 hydroxyalkyl and C.sub.1-6 alkoxy; R.sub.1 is selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, C.sub.1-6 alkyl, C.sub.3-8 cycloalkyl and 3 to 6 membered heterocyclyl, which is optionally further substituted by one or more substituents selected from the group consisting of deuterium, halogen, amino, nitro, hydroxy, cyano, C.sub.1-6 alkyl, C.sub.3-8 cycloalkyl and 3 to 6 membered heterocyclyl; R.sub.11 and R.sub.12 are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl and —(CH.sub.2).sub.nNR.sub.aaR.sub.bb; R.sub.aa and R.sub.bb are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, C.sub.1-6 alkyl, C.sub.1-6 alkoxy and 3 to 6 membered heterocyclyl; n is an integer of 0, 1, 2 or 3; or, R.sub.11 and R.sub.12, together with the nitrogen atom to which they are attached, form a 4 to 12 membered heterocyclyl, which is optionally substituted by one or more substituents selected from the group consisting of halogen, hydroxy, amino, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, amino substituted by C.sub.1-6 alkyl, C.sub.3-12 cycloalkyl and substituted or unsubstituted 3 to 12 membered heterocyclyl; each R.sup.1 is independently selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, C.sub.1-6 alkyl, C.sub.1-6 alkoxy, C.sub.1-6 hydroxyalkyl and C.sub.3-8 cycloalkyl.

    35. (canceled)

    36. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein the compound has the following structure: ##STR00318## ##STR00319## ##STR00320## ##STR00321## ##STR00322## ##STR00323## ##STR00324## ##STR00325## ##STR00326## ##STR00327## ##STR00328## ##STR00329## ##STR00330## ##STR00331## ##STR00332## ##STR00333## ##STR00334## ##STR00335## ##STR00336## ##STR00337## ##STR00338## ##STR00339## ##STR00340## ##STR00341## ##STR00342## ##STR00343## ##STR00344## ##STR00345## ##STR00346## ##STR00347## ##STR00348## ##STR00349## ##STR00350## ##STR00351## ##STR00352## ##STR00353##

    37. A method for preparing the compound of formula (V), a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 31, characterized by comprising the following steps of: ##STR00354## reacting a compound of formula (V-1) with a compound of formula (V-2) to obtain a compound of formula (V-3); then subjecting the compound of formula (V-3) to a reduction reaction to obtain a compound of formula (V-4); then reacting the compound of formula (V-4) with a compound of formula (V-5) to obtain the compound of formula (V), a stereoisomer thereof or a pharmaceutically acceptable salt thereof, wherein: X.sub.1 is selected from the group consisting of halogen; X.sub.2 is selected from the group consisting of halogen; R.sub.1, R.sub.2, R.sub.5, R.sub.6 and R.sub.10 are as defined in claim 31; or, characterized by comprising the following step of: ##STR00355## reacting a compound of formula (V-6) with a compound of formula (V-7) to obtain the compound of formula (V-1); then subjecting the compound of formula (V-1) to reactions to obtain the compound of formula (V), a stereoisomer thereof or a pharmaceutically acceptable salt thereof; wherein: X.sub.3 is selected from the group consisting of halogen.

    38. (canceled)

    39. A method for preparing the compound of formula (VII), a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 33, characterized by comprising the following steps of: ##STR00356## reacting a compound of formula (VII-1) with a compound of formula (VII-2) to obtain a compound of formula (VII-3); then subjecting the compound of formula (VII-3) to a reduction reaction to obtain a compound of formula (VII-4); then reacting the compound of formula (VII-4) with a compound of formula (VII-5) to obtain the compound of formula (VII), a stereoisomer thereof or a pharmaceutically acceptable salt thereof, wherein: X.sub.1 is selected from the group consisting of halogen; X.sub.2 is selected from the group consisting of halogen; R.sup.1, R.sub.1, R.sub.11, R.sub.12 and y are as defined in claim 33.

    40. A method for preparing the compound of formula (VIII), a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 34, characterized by comprising the following steps of: ##STR00357## wherein: X.sub.1 is selected from the group consisting of halogen; X.sub.2 and X.sub.3 are each independently selected from the group consisting of halogen; R.sup.1, R.sub.1, R.sub.2, R.sub.11, R.sub.12 and y are as defined in claim 34.

    41. A pharmaceutical composition comprising a therapeutically effective dose of the compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, and one or more pharmaceutically acceptable carriers, diluents or excipients.

    42. A method of treating a cancer-related disease in a mammal in need thereof, the method comprising administering to the mammal a therapeutically effective amount of the compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1.

    43. (canceled)

    44. A method of treating cancer, inflammation, chronic liver disease, diabetes, cardiovascular disease or AIDS-related disease in a mammal in need thereof, the method comprising administering to the mammal a therapeutically effective amount of the compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein the cancer, inflammation, chronic liver disease, diabetes, cardiovascular disease or AIDS-related disease is disease mediated by HER2 exon 20 mutation and/or EGFR exon 20 mutation.

    45. The method according to claim 44, wherein the cancer is selected from the group consisting of breast cancer, cervical cancer, colon cancer, lung cancer, stomach cancer, rectal cancer, pancreatic cancer, brain cancer, liver cancer, solid tumor, glioma, glioblastoma, leukemia, lymphoma, myeloma and non-small cell lung cancer.

    46. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 33, wherein R.sub.1 is selected from the group consisting of C.sub.1-3 alkyl, C.sub.3-6 cycloalkyl and 3 to 6 membered heterocyclyl containing 1 to 2 atoms selected from the group consisting of N, O and S, which is optionally further substituted by one or more substituents selected from the group consisting of deuterium, halogen, amino, nitro, hydroxy, cyano, C.sub.1-3 alkyl, C.sub.3-6 cycloalkyl and 3 to 6 membered heterocyclyl.

    47. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 33, wherein R.sub.1 is selected from the group consisting of methyl, ethyl, propyl, isopropyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclopropoxy, cyclobutoxy, tetrahydrofuranyl, pyranyl, aziridinyl, azetidinyl, pyrrolyl, piperidinyl and tetrahydrothienyl, which is optionally further substituted by one or more substituents selected from the group consisting of deuterium, fluorine, chlorine, bromine, amino, nitro, hydroxy, cyano, methyl, ethyl, propyl and isopropyl.

    48. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 33, wherein R.sub.aa and R.sub.bb are each independently selected from the group consisting of hydrogen, deuterium, halogen, cyano, amino, nitro, hydroxy, methyl, ethyl, propyl, isopropyl, methoxy, ethoxy, propoxy, isopropoxy and 5 to 6 membered heterocyclyl containing 1 to 2 atoms selected from the group consisting of N and O atom.

    49. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 33, wherein R.sup.1 is selected from the group consisting of hydrogen, deuterium, fluorine, chlorine, bromine, amino, nitro, hydroxy, cyano, methyl, ethyl, propyl, isopropyl, hydroxymethyl, hydroxyethyl, hydroxypropyl, isohydroxypropyl, methoxy, ethoxy, propoxy, isopropoxy, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

    50. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein ring A is selected from the group consisting of phenyl, benzoheteroaryl, 3 to 8 membered heterocyclyl and 5 to 10 membered heteroaryl.

    51. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.1 is selected from the group consisting of hydrogen, methyl, ethyl, propyl, butyl, tri-deuterated methyl, methyl substituted by one to three of fluorine, methyl substituted by one to three of chlorine, methyl substituted by one to three of bromine, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, ##STR00358##

    52. The compound, a stereoisomer thereof or a pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.3 is selected from the group consisting of methyl, ethyl, propyl, methoxy, ethoxy, propoxy, methoxy substituted by 1 to 3 fluorine, methoxy substituted by 1 to 3 chlorine, methoxy substituted by 1 to 3 bromine, —NHC(O)C≡CH, —NHC(O)C≡CCH.sub.3, —NHC(O)CH═CH.sub.2, —NHC(O)CF═CH.sub.2, —NHC(O)C(CH.sub.3)═CH.sub.2, —NHC(O)CH═CHCH.sub.3, —NHC(O)CH═CHCH.sub.2N(CH.sub.3).sub.2, ##STR00359## ##STR00360## ##STR00361##

    Description

    DETAILED DESCRIPTION

    [0220] The present invention will be further described with reference to the following examples, but the examples should not be considered as limiting the scope of the present invention.

    EXAMPLES

    [0221] The structures of the compounds of the present invention were identified by nuclear magnetic resonance (NMR) and/or liquid chromatography-mass spectrometry (LC-MS). NMR shifts (δ) are given in parts per million (ppm). NMR was determined by a Bruker AVANCE-400 instrument. The solvents for determination were deuterated-dimethyl sulfoxide (DMSO-d.sub.6), deuterated-methanol (CD.sub.3OD) and deuterated-chloroform (CDCl.sub.3), and the internal standard was tetramethylsilane (TMS).

    [0222] Liquid chromatography-mass spectrometry (LC-MS) was determined on an Agilent 1200 Infinity Series mass spectrometer. High performance liquid chromatography (HPLC) was determined on an Agilent 1200DAD high pressure liquid chromatograph (Sunfire C18 150×4.6 mm column), and a Waters 2695-2996 high pressure liquid chromatograph (Gimini C18 150×4.6 mm column).

    [0223] Yantai Huanghai HSGF254 or Qingdao GF254 silica gel plate was used as the thin-layer silica gel chromatography (TLC) plate. The dimension of the silica gel plate used in TLC was 0.15 mm to 0.2 mm, and the dimension of the silica gel plate used in product purification was 0.4 mm to 0.5 mm. Yantai Huanghai 200 to 300 mesh silica gel was generally used as a carrier for column chromatography.

    [0224] The raw materials used in the examples of the present invention are known and commercially available, or can be synthesized by or according to known methods in the art.

    [0225] Unless otherwise stated, all reactions of the present invention were carried out under continuous magnetic stirring under a dry nitrogen or argon atmosphere, the solvent was dry, and the reaction temperature was in degrees celsius.

    Example 1

    Isopropyl 2-((5-(but-2-ynamido)-2-methoxy-4-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0226] ##STR00055##

    Step 1: Methyl 2-chloro-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0227] ##STR00056##

    [0228] Aluminum trichloride (6.4 g, 48 mmol) was added in batches to a solution of methyl 2,4-dichloropyrimidine-5-carboxylate (5.0 g, 24 mmol) in 1,2-dichloroethane (50 mL) at 0° C. The reaction solution was stirred at room temperature for 15 minutes, followed by the addition of 1-methyl-1H-indole (3.2 g, 24 mmol). The reaction solution was heated to 55° C. and stirred for 1.5 hours. The reaction solution was cooled to 0° C., to which methanol (10 mL) and water (30 mL) were added slowly. The solution was stirred at room temperature for 30 minutes, and extracted with dichloromethane (30 mL*2). The organic phases were combined, dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by column chromatography (petroleumether:ethylacetate=1:1) to obtain compound 1-1 (5.0 g, yield: 69%).

    [0229] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 8.83 (s, 1H), 8.17 (dd, J=6.8, 1.8 Hz, 1H), 7.97 (s, 1H), 7.48-7.27 (m, 3H), 3.88 (s, 3H), 3.86 (s, 3H).

    Step 2: Methyl 2-((4-fluoro-2-methoxy-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0230] ##STR00057##

    [0231] P-toluenesulfonic acid monohydrate (3.8 g, 19.9 mmol) was added to a solution of 4-fluoro-2-methoxy-5-nitroaniline (1.1 g, 5.97 mmol) and compound 1-1 (1.5 g, 4.97 mmol) in dioxane (50 mL). The reaction solution was heated to 100° C. and reacted for 16 hours. The reaction solution was cooled, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by column chromatography (dichloromethane:methanol:ammonia (w/w 25%)=100:2:0.5%) to obtain compound 1-2 (1.8 g, yield: 80%).

    [0232] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.34 (br, 1H), 8.80 (s, 1H), 8.27 (s, 1H), 7.85 (m, 1H), 7.64 (d, J=8.0 Hz, 1H), 7.47-7.36 (m, 1H), 7.37-7.29 (m, 1H), 7.24-7.13 (m, 1H), 6.81 (d, J=12.1 Hz, 1H), 4.01 (s, 3H), 3.94 (d, J=2.9 Hz, 3H), 3.79 (s, 3H).

    Step 3: Methyl 2-((2-methoxy-4-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0233] ##STR00058##

    [0234] Potassium carbonate (413 mg, 2.99 mmol) was added to a solution of N-methyl-1-(1-methylpyrrolidin-2-yl)methanamine hydrochloride (180 mg, 1.19 mmol) and compound 1-2 (450 mg, 0.99 mmol) in acetonitrile (15 mL). The reaction solution was heated to 80° C. and reacted for 2 hours. The reaction solution was cooled, and concentrated to dryness. The resulting residues were dissolved in dichloromethane (30 mL), and washed with brine (20 mL*3). The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by column chromatography (dichloromethane:methanol:ammonia (w/w 25%)=100:5:0.5%) to obtain compound 1-3 (450 mg, yield: 81%).

    [0235] MS m/z (ESI): 560.2[M+H].sup.+.

    Step 4: Isopropyl 2-((2-methoxy-4-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0236] ##STR00059##

    [0237] Sodium hydride (19 mg, 0.46 mmol, w/w 60%) was added to a solution of compound 1-3 (200 mg, 0.36 mmol) in isopropanol (6 mL). The reaction solution was heated to 80° C. and reacted for 30 minutes. The reaction solution was concentrated to dryness to obtain the crude compound 1-4 (200 mg), which was used directly in the next step.

    [0238] MS m/z (ESI): 588.3[M+H].sup.+.

    Step 5: Isopropyl 2-((5-amino-2-methoxy-4-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0239] ##STR00060##

    [0240] Zinc powder (111 mg, 1.7 mmol) was added to a solution of compound 1-4 (250 mg, 0.43 mmol) and ammonium chloride (227 mg, 4.25 mmol) in acetone (10 mL) and water (1 mL). The reaction solution was stirred at room temperature for 40 minutes. The reaction solution was filtered. The organic phase was added with dichloromethane (30 mL), and washed with water (15 mL*2). The solid was rinsed with dichloromethane (30 mL). The organic phases were combined, dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by column chromatography (dichloromethane:methanol:ammonia (w/w 25%)=100:10:0.5%) to obtain compound 1-5 (140 mg, yield: 59%).

    [0241] MS m/z (ESI): 558.3[M+H].sup.+.

    Step 6: Isopropyl 2-((5-(but-2-ynamido)-2-methoxy-4-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0242] ##STR00061##

    [0243] 2-(7-Azabenzotriazol)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (102 mg, 0.27 mmol) was added to a solution of compound 1-5 (100 mg, 0.18 mmol), 2-butynoic acid (23 mg, 0.27 mmol) and N,N-diisopropylethylamine (70 mg, 0.54 mmol) in dichloromethane (5 mL). The reaction solution was stirred at room temperature for 16 hours. The reaction solution was washed with water (15 mL*2). The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by thin layer chromatography (dichloromethane:methanol:ammonia (w/w 25%)=100:5:0.5%) to obtain the product (26 mg, yield: 24%).

    [0244] MS m/z (ESI): 624.3[M+H].sup.+.

    [0245] .sup.1H NMR (400 MHz, Methanol-d4) δ 9.23 (s, 1H), 8.70 (s, 1H), 8.26-8.08 (m, 1H), 7.68-7.52 (m, 1H), 7.42 (d, J=8.1 Hz, 1H), 7.21 (t, J=7.5 Hz, 1H), 7.10 (t, J=7.5 Hz, 1H), 6.90 (s, 1H), 5.05-4.93 (m, 1H), 4.02-3.79 (m, 6H), 3.24-3.12 (m, 1H), 3.00-2.84 (m, 1H), 2.83-2.74 (m, 1H), 2.71 (s, 3H), 2.68-2.58 (m, 1H), 2.49 (s, 3H), 2.43-2.30 (m, 1H), 2.10-1.93 (m, 4H), 1.83-1.69 (m, 2H), 1.59-1.41 (m, 1H), 1.16-0.95 (m, 6H).

    Example 2

    Isopropyl 2-((5-acrylamido-2-methoxy-4-(9-methyl-3,9-diazaspiro[5.5]undecan-3-yl)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0246] ##STR00062## ##STR00063##

    Step 1: Methyl 2-((2-methoxy-4-(9-methyl-3,9-diazaspiro[5.5]undecan-3-yl)-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0247] ##STR00064##

    [0248] This step was carried out in accordance with Step 3 of Example 1 (260 mg, yield: 78%).

    [0249] MS m/z (ESI): 600.3[M+H].sup.+.

    Step 2: Isopropyl 2-((2-methoxy-4-(9-methyl-3,9-diazaspiro[5.5]undecan-3-yl)-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0250] ##STR00065##

    [0251] This step was carried out in accordance with Step 4 of Example 1 (260 mg, crude product).

    [0252] MS m/z (ESI): 628.3[M+H].sup.+.

    Step 3: Isopropyl 2-((5-amino-2-methoxy-4-(9-methyl-3,9-diazaspiro[5.5]undecan-3-yl)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0253] ##STR00066##

    [0254] This step was carried out in accordance with Step 5 of Example 1 (220 mg, yield: 89%).

    [0255] MS m/z (ESI): 598.3[M+H].sup.+.

    Step 4: Isopropyl 2-((5-acrylamido-2-methoxy-4-(9-methyl-3,9-diazaspiro[5.5]undecan-3-yl)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0256] ##STR00067##

    [0257] Acryloyl chloride (10 mg, 0.11 mmol) and triethylamine (20 mg, 0.20 mmol) were added to a solution of compound 1-5 (60 mg, 0.10 mmol) in dichloromethane (5 mL). The reaction solution was stirred at room temperature for 16 hours. The reaction solution was concentrated under reduced pressure, and the resulting residues were purified by thin layer chromatography (dichloromethane:methanol:ammonia (w/w 25%)=100:5:0.5) to obtain the product (26 mg, yield: 40%).

    [0258] MS m/z (ESI): 652.3[M+H].sup.+.

    Example 3

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((4aR,7aR)-1-methyloctahydro-6H-pyrrolo[3,4-b]pyridin-6-yl)phenyl)acrylamide

    [0259] ##STR00068## ##STR00069##

    Step 1: 3-(5-Bromo-2-chloropyrimidin-4-yl)-1-cyclopropyl-1H-indole (Intermediate 3-1)

    [0260] ##STR00070##

    [0261] Aluminum trichloride (2.66 g, 20 mmol) was added in batches to a solution of 5-bromo-2,4-dichloropyrimidine (2.28 g, 10 mmol) in 1,2-dichloroethane (30 mL) at 0° C. The reaction solution was stirred at room temperature for 15 minutes, followed by the addition of 1-cyclopropyl-1H-indole (1.57 g, 10 mmol). The reaction solution was heated to 60° C. and stirred for 2 hours. The reaction solution was cooled to 0° C., to which methanol (10 mL) and water (30 mL) were added slowly. The solution was stirred at room temperature for 30 minutes, and extracted with dichloromethane (20 mL*3). The organic phases were combined, dried over anhydrous sodium sulfate, and concentrated to dryness. The resulting residues were purified by column chromatography (petroleumether:ethyl acetate=2:3) to obtain intermediate 3-1 (2.71 g, yield: 78%).

    [0262] MS m/z (ESI): 348.1[M+H].sup.+.

    Step 2: 5-Bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(4-fluoro-2-methoxy-5-nitrophenyl)pyrimidin-2-amine (Intermediate 3-2)

    [0263] ##STR00071##

    [0264] P-toluenesulfonic acid monohydrate (1.90 g, 10 mmol) was added to a solution of intermediate 3-2 (1.12 g, 3.2 mmol) and 4-fluoro-2-methoxy-5-nitroaniline (707 mg, 3.8 mmol) in dioxane (30 mL). The reaction solution was heated to 100° C. and reacted for 16 hours. The reaction solution was cooled, and concentrated to dryness. The resulting residues were purified by column chromatography (dichloromethane:methanol:ammonia (w/w 25%)=100:2:0.5) to obtain intermediate 3-2 (1.15 g, yield: 72%).

    [0265] MS m/z (ESI): 498.2[M+H].sup.+.

    Step 3: Tert-butyl (4aR,7aR)-octahydro-6H-pyrrolo[3,4-b]pyridine-6-carboxylate (Intermediate 3-3)

    [0266] ##STR00072##

    [0267] A solution of di-tert-butyl dicarbonate (8.29 g, 38 mmol) in dichloromethane (20 mL) was slowly added dropwise to a solution of (4aR,7aR)-octahydro-1H-pyrrolo[3,4-b]pyridine (5.00 g, 40 mmol) in dichloromethane (800 mL) under an ice-water bath. After completion of the addition, the reaction solution was reacted for 16 hours. The reaction solution was concentrated, and the resulting residues were purified by column chromatography (dichloromethane:methanol:concentrated ammonia=gradient elution from 99:1:0.1 to 90:10:1) to obtain intermediate 3-3 (7.35 g, yield: 82%).

    [0268] MS m/z (ESI): 227.1[M+H].sup.+.

    Step 4: Tert-butyl (4aR,7aR)-1-methyloctahydro-6H-pyrrolo[3,4-b]pyridine-6-carboxylate (Intermediate 3-4)

    [0269] ##STR00073##

    [0270] Methyl iodide (2.84 g, 20 mmol) was slowly added dropwise to a solution of intermediate 3-3 (5.00 g, 22 mmol) and triethylamine (5.06 g, 50 mmol) in dichloromethane (100 mL) under an ice-water bath. After completion of the addition, the reaction solution was slowly warmed up to room temperature and reacted for 16 hours. The reaction solution was concentrated, and the resulting residues were purified by column chromatography (dichloromethane:methanol:concentrated ammonia=gradient elution from 99:1:0.1 to 90:10:1) to obtain intermediate 3-4 (3.28 g, yield: 62%).

    [0271] MS m/z (ESI): 241.1[M+H].sup.+.

    Step 5: Tert-butyl (4aR,7aR)-1-Methyloctahydro-1H-pyrrolo[3,4-b]pyridine-6-carboxylate (Intermediate 3-5)

    [0272] ##STR00074##

    [0273] Intermediate 3-4 (3.28 g, 14 mmol) was dissolved in dichloromethane (50 mL) under a water bath, to which 2M hydrogen chloride/ether solution (25 mL, 50 mmol) was slowly added dropwise. After completion of the addition, the reaction solution was reacted for 4 hours. The reaction solution was filtered, and the solid was rinsed with anhydrous ether (10 mL*2). The solid was dried under vacuum to obtain the hydrochloride intermediate 3-5 (2.07 g, yield: 71%).

    [0274] MS m/z (ESI): 141.3[M+H].sup.+.

    Step 6: 5-Bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((4aR,7aR)-1-methyloctahydro-6H-pyrrolo[3,4-b]pyridin-6-yl)-5-nitrophenyl)pyrimidin-2-amine (Intermediate 3-6)

    [0275] ##STR00075##

    [0276] Potassium carbonate (690 mg, 5.0 mmol) was added to a solution of intermediate 3-2 (498 mg, 1.0 mmol) and intermediate 3-5 (298 mg, 1.4 mmol) in acetonitrile (30 mL). The reaction solution was reacted under reflux for 16 hours. The reaction solution was cooled, and concentrated to dryness. The resulting residues were dissolved in dichloromethane (50 mL), and washed with brine (30 mL*3). The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness. The resulting crude product was purified by column chromatography (dichloromethane:methanol:concentrated ammonia=gradient elution from 99:1:0.1 to 95:5:0.5) to obtain intermediate 3-6 (420 mg, yield: 68%).

    [0277] MS m/z (ESI): 618.3 [M+H].sup.+.

    Step 7: 4-(1-Cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((4aR,7aR)-1-methyloctahydro-6H-pyrrolo[3,4-b]pyridin-6-yl)-5-nitrophenyl)-5-(oxazol-2-yl)pyrimidin-2-amine (Intermediate 3-7)

    [0278] ##STR00076##

    [0279] Intermediate 3-6 (200 mg, 0.3 mmol), 2-(tri-n-butylstannyl)oxazole (127 mg, 0.36 mmol) and tetrakis(triphenylphosphine) palladium (30 mg) were dissolved in toluene (3 mL) under a nitrogen atmosphere. The reaction solution was reacted at 110° C. overnight. The reaction solution was concentrated under reduced pressure, and the resulting crude product was purified by column chromatography (dichloromethane:methanol:concentrated ammonia=gradient elution from 99:1:0.1 to 94:6:0.6) to obtain intermediate 3-7 (145 mg, yield: 74%).

    [0280] MS m/z (ESI): 607.3 [M+H].sup.+.

    Step 8: N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((4aR,7aR)-1-methyloctahydro-6H-pyrrolo[3,4-b]pyridin-1-yl)phenyl)acrylamide (compound 3)

    [0281] ##STR00077##

    [0282] Zinc powder (45 mg, 0.70 mmol) was added to a solution of compound 3-7 (140 mg, 0.23 mmol) and ammonium chloride (123 mg, 2.3 mmol) in acetone (4 mL)/water (1 mL) under stirring at room temperature. The reaction solution was reacted at room temperature for 2 hours. The reaction solution was filtered, the solid was rinsed with dichloromethane (10 mL*2) and water (5 mL*2) successively, and the filtrate was partitioned. The organic phase was washed with water (5 mL*2), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated to obtain the crude intermediate 3-8. The crude intermediate 3-8 was dissolved in dichloromethane (5 mL), followed by the successive addition of triethylamine (50 mg, 0.50 mmol) and acryloyl chloride (23 mg, 0.25 mmol) under stirring at room temperature. The reaction solution was stirred at room temperature for 16 hours. The reaction solution was concentrated under reduced pressure, and the resulting residues were purified by column chromatography (dichloromethane:methanol:ammonia (w/w 25%)=99:1:0.1 to 93:7:0.7) to obtain compound 3 (59 mg, yield: 40%).

    [0283] MS m/z (ESI): 631.2 [M+H].sup.+.

    Example 4

    Isopropyl 2-((5-acrylamido-4-(5-amino-5-methylhexahydrocyclopenta[c]pyrrol-2(1H)-yl)-2-methoxyphenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0284] ##STR00078## ##STR00079##

    Step 1: Benzyl 5-hydroxy-5-methylhexahydrocyclopenta[c]pyrrole-2(1H)-carboxylate (Intermediate 4-1)

    [0285] ##STR00080##

    [0286] Benzyl 5-oxohexahyrdocyclopenta[c]pyrrole-2(1H)-carboxylate (1.20 g, 4.63 mmol) was dissolved in tetrahydrofuran (20 mL) at −78° C. under a nitrogen atmosphere, and methylmagnesium bromide (3.0 M ether solution, 2.5 mL, 7.5 mmol) was added dropwise. The reaction solution was stirred at −78° C. for two hours, and TLC indicated that the reaction was completed. Saturated ammonium chloride solution (15 mL) was added to quench the reaction, and the solution was extracted with ethyl acetate (25 mL*3). The organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain the crude intermediate 4-1 (1.21 g, colorless oil), which was used directly in the next step without further purification.

    [0287] MS m/z (ESI): 276.1 [M+H].sup.+.

    Step 2: Benzyl 5-formamido-5-methylhexahydrocyclopenta[c]pyrrole-2(1H)-carboxylate (Intermediate 4-2)

    [0288] ##STR00081##

    [0289] Intermediate 4-1 (1.21 g, 4.97 mmol) was dissolved in acetic acid (6 mL) at room temperature, and then the reaction system was cooled to 10° C. Trimethylsilyl cyanide (0.54 g, 5.47 mmol) was added, and then concentrated sulfuric acid (3 mL) was added dropwise. The reaction solution was kept below 10° C., and reacted for 4 hours. 10% sodium hydroxide solution was added to quench the reaction, and the reaction solution was extracted with dichloromethane (20 mL*3). The organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain the crude intermediate 4-2 (1.10 g).

    [0290] MS m/z (ESI): 303.1 [M+H].sup.+.

    Step 3: Benzyl 5-amino-5-methylhexahydrocyclopenta[c]pyrrole-2(1H)-carboxylate (Intermediate 4-3)

    [0291] ##STR00082##

    [0292] Intermediate 4-2 (1.10 g) was dissolved in ethanol (15 mL) at room temperature, followed by the addition of aqueous sodium hydroxide solution (1.50 g of sodium hydroxide dissolved in 10 mL of water). The reaction solution was heated to 75° C., and reacted for two hours. After completion of the reaction, ethanol was removed under reduced pressure, and 6N hydrochloric acid was added to adjust the pH to 2. The solution was washed with dichloromethane (25 mL*2) and partitioned. 10% sodium hydroxide solution was added to the aqueous phase to adjust the pH to 12, and the aqueous phase was extracted with dichloromethane (50 mL*3). The organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting residues were purified by column chromatography (dichloromethane:methanol:ammonia (w/w 25%)=99:1:0.1 to 92:8:0.8) to obtain intermediate 4-3 (0.60 g, yield of three steps: 47%).

    [0293] MS m/z (ESI): 275.1 [M+H].sup.+.

    Step 4: 5-Methyloctahydrocyclopenta[c]pyrrol-5-amine (Intermediate 4-4)

    [0294] ##STR00083##

    [0295] Intermediate 4-3 (600 mg) was dissolved in methanol (10 mL) at room temperature, followed by the addition of 10% palladium on carbon (120 mg). A hydrogenation reaction was carried out at room temperature for 16 hours. After completion of the reaction, the reaction solution was filtered, and the resulting solid was rinsed with dichloromethane (3 mL*2). The filtrate was concentrated under reduced pressure to obtain intermediate 4-4 (302 mg, yield: 98%).

    [0296] MS m/z (ESI): 141.1 [M+H].sup.+.

    Step 5: Methyl 2-((4-(5-((tert-butoxycarbonyl)amino)-5-methylhexahydrocyclopenta[c]pyrrol-2(1H)-yl)-2-methoxy-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate (Intermediate 4-6)

    [0297] ##STR00084##

    [0298] Potassium carbonate (415 mg, 3.0 mmol) was added to a solution of intermediate 1-2 (451 mg, 1.0 mmol) and intermediate 4-4 (168 mg, 1.2 mmol) in acetonitrile (15 mL). The reaction solution was reacted under reflux for 16 hours. The reaction solution was cooled and filtered, and the resulting solid was rinsed with dichloromethane (10 mL*2). The filtrates were combined and concentrated under reduced pressure to obtain the crude intermediate 4-5. The crude product was dissolved in dichloromethane (25 mL), followed by the addition of Boc.sub.2O (500 mg). The reaction solution was stirred at room temperature for 16 hours. The reaction solution was concentrated, and the resulting residues were purified by column chromatography (dichloromethane:methanol:concentrated ammonia=gradient elution from 99:1:0.1 to 95:5:0.5) to obtain intermediate 4-6 (511 mg, yield: 76%).

    [0299] MS m/z (ESI): 672.4 [M+H].sup.+.

    Step 6: Isopropyl 2-((4-(5-((tert-butoxycarbonyl)amino)-5-methylhexahydrocyclopenta[c]pyrrol-2(1H)-yl)-2-methoxy-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate (Intermediate 4-7)

    [0300] ##STR00085##

    [0301] Sodium hydride (25 mg, 0.62 mmol, w/w 60%) was added to a solution of compound 4-6 (150 mg, 0.22 mmol) in isopropanol (5 mL). The reaction solution was reacted under reflux for 1 hour. The reaction solution was concentrated to dryness to obtain the crude compound 1-4 (174 mg), which was used directly in the next step.

    [0302] MS m/z (ESI): 700.2[M+H].sup.+.

    Step 7: Isopropyl 2-((5-acrylamido-4-(5-((tert-butoxycarbonyl)amino)-5-methylhexahydrocyclopenta[c]pyrrol-2(1H)-yl)-2-methoxyphenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate (Intermediate 4-9)

    [0303] ##STR00086##

    [0304] Zinc powder (100 mg, 1.5 mmol) was added to a solution of compound 4-7 (174 mg, crude product) and ammonium chloride (267 mg, 5 mmol) in acetone (10 mL)/water (2 mL) at room temperature under stirring. The reaction solution was reacted at room temperature for 2 hours. The reaction solution was filtered, the solid was rinsed with dichloromethane (30 mL*2) and water (15 mL*2) successively, and the filtrate was partitioned. The organic phase was washed with water (10 mL*2), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated to obtain the crude intermediate 4-8. The crude intermediate 4-8 was dissolved in dichloromethane (10 mL), followed by the successive addition of triethylamine (50 mg, 0.50 mmol) and acryloyl chloride (27 mg, 0.30 mmol) under stirring at room temperature. The reaction solution was stirred at room temperature for 16 hours. The reaction solution was concentrated under reduced pressure, and the resulting residues were purified by preparative HPLC to obtain intermediate 4-9 (63 mg, yield of three steps: 39%).

    [0305] MS m/z (ESI): 724.1 [M+H].sup.+.

    Step 8: Isopropyl 2-((5-acrylamido-4-(5-amino-5-methylhexahydrocyclopenta[c]pyrrol-2(1H)-yl)-2-methoxyphenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate hydrochloride (Hydrochloride of Compound 4)

    [0306] ##STR00087##

    [0307] Intermediate 4-9 (60 mg) was dissolved in dichloromethane (2 mL) at room temperature, followed by the addition of 2M solution (1 mL) of hydrogen chloride in ether. The reaction solution was stirred at room temperature for two hours. After completion of the reaction, the reaction solution was filtered. The resulting solid was rinsed with dichloromethane (1 mL*2), and dried under vacuum to obtain the hydrochloride of compound 4 (39 mg, yield: 71%).

    [0308] MS m/z (ESI): 624.3 [M+H].sup.+.

    Example 5

    N-(4-Fluorobenzyl)-2-((5-methacrylamido-2-methyl-4-(4-methylpiperazin-1-yl)phenyl) amino)-N-methyl-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxamide

    [0309] ##STR00088## ##STR00089##

    Step 1: Methyl 2-((2-methoxy-4-(4-methylpiperazin-1-yl)-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate (Intermediate 5-1)

    [0310] ##STR00090##

    [0311] Potassium carbonate (415 mg, 3.0 mmol) was added to a solution of intermediate 1-2 (451 mg, 1.0 mmol) and methylpiperazine (120 mg, 1.2 mmol) in acetonitrile (15 mL). The reaction solution was reacted under reflux for 16 hours. The reaction solution was cooled and filtered, and the resulting solid was rinsed with dichloromethane (10 mL*2). The filtrates were combined and concentrated under reduced pressure. The resulting residues were purified by column chromatography (dichloromethane:methanol:concentrated ammonia=gradient elution from 99:1:0.1 to 95:5:0.5) to obtain intermediate 5-1 (401 mg, yield: 75%).

    [0312] MS m/z (ESI): 532.3[M+H].sup.+.

    Step 2: 2-((2-Methoxy-4-(4-methylpiperazin-1-yl)-5-nitrophenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylic acid (Intermediate 5-2)

    [0313] ##STR00091##

    [0314] Intermediate 5-1 (400 mg, 0.75 mmol) was dissolved in tetrahydrofuran (5 mL) at room temperature, followed by the addition of lithium hydroxide monohydrate (42 mg, 1.0 mmol). The reaction solution was stirred at room temperature for 16 hours. The reaction solution was directly purified by preparative HPLC to obtain compound 5-2 (286 mg, yield: 73%).

    [0315] MS m/z (ESI): 518.3[M+H].sup.+.

    Step 3: N-(4-Fluorobenzyl)-2-((2-methoxy-4-(4-methylpiperazin-1-yl)-5-nitrophenyl)amino)-N-methyl-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxamide

    [0316] ##STR00092##

    [0317] Intermediate 5-2 (200 mg, 0.39 mmol), 1-(4-fluorophenyl)-N-methylmethanamine (60 mg, 0.43 mmol), triethylamine (101 mg, 1.0 mmol) and 2-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (190 mg, 0.50 mmol) were dissolved in dichloromethane (10 mL) at room temperature. The reaction solution was stirred at room temperature for 16 hours. The reaction solution was concentrated, and the resulting residues were purified by column chromatography (dichloromethane:methanol:concentrated ammonia=gradient elution from 99:1:0.1 to 94:6:0.6) to obtain intermediate 5-3 (143 mg, yield: 58%).

    [0318] MS m/z (ESI): 639.1[M+H].sup.+.

    Step 4: N-(4-Fluorobenzyl)-2-((5-methacrylamido-2-methyl-4-(4-methylpiperazin-1-yl)phenyl)amino)-N-methyl-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxamide (compound 5)

    [0319] ##STR00093##

    [0320] Zinc powder (40 mg, 0.62 mmol) was added to a solution of compound 5-3 (140 mg, 0.22 mmol) and ammonium chloride (118 mg, 2.2 mmol) in acetone (3 mL)/water (1 mL) under stirring at room temperature. The reaction solution was reacted at room temperature for 2 hours. The reaction solution was filtered, the solid was rinsed with dichloromethane (10 mL*2) and water (5 mL*2) successively, and the filtrate was partitioned. The organic phase was washed with water (5 mL*2), dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated to obtain the crude intermediate 5-4. The crude intermediate 5-4 was dissolved in dichloromethane (3 mL), followed by the successive addition of triethylamine (50 mg, 0.50 mmol) and methacryloyl chloride (31 mg, 0.30 mmol) under stirring at room temperature. The reaction solution was stirred at room temperature for 16 hours. The reaction solution was concentrated under reduced pressure, and the resulting residues were purified by preparative HPLC to obtain compound 5 (45 mg, yield: 31%).

    [0321] MS m/z (ESI): 661.3 [M+H].sup.+.

    Example 6

    Trans-N-(2-((2-(dimethylamino)ethyl)(methyl)amino)-5-((5-(3-(4-fluorophenyl)acrylamido)-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-(trifluoromethoxy)phenyl)but-2-ynamide

    [0322] ##STR00094## ##STR00095##

    Step 1: Trans-N-(2,4-dichloropyrimidin-5-yl)-3-(4-fluorophenyl)acrylamide

    [0323] ##STR00096##

    [0324] 2,4-Dichloropyrimidin-5-amine (1.64 g, 10 mmol) and triethylamine (1.52 g, 15 mmol) were dissolved in dichloromethane (50 mL), followed by the addition of p-fluorocinnamyl chloride (1.84 g, 10 mmol) at room temperature. The reaction solution was stirred at room temperature for 2 hours. The reaction solution was concentrated, and the resulting residues were purified by column chromatography (petroleumether:ethylacetate=90:10 to 20:80) to obtain intermediate 6-1 (2.14 g, yield: 68%).

    [0325] MS m/z (ESI): 312.2[M+H].sup.+.

    Step 2: Trans-N-(2-chloro-4-(1-methyl-1H-indol-3-yl)pyrimidin-5-yl)-3-(4-fluorophenyl)acrylamide

    [0326] ##STR00097##

    [0327] Aluminum trichloride (853 mg, 6.4 mmol) was added in batches to a solution of methyl 2,4-dichloropyrimidine-5-carboxylate (1.00 g, 3.2 mmol) in 1,2-dichloroethane (20 mL) at 0° C. The reaction solution was stirred at room temperature for 15 minutes, followed by the addition of 1-methyl-1H-indole (420 mg, 3.2 mmol). The reaction solution was heated to 55° C. and stirred for 1.5 hours. The reaction solution was cooled to 0° C., to which methanol (5 mL) and water (20 mL) were added slowly. The solution was stirred at room temperature for 30 minutes, and extracted with dichloromethane (20 mL*2). The organic phases were combined, dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by column chromatography (petroleumether:ethylacetate=1:1) to obtain compound 6-2 (814 mg, yield: 62%).

    [0328] MS m/z (ESI): 407.1[M+H].sup.+.

    Step 3: Trans-N-(2-((4-fluoro-5-nitro-2-(trifluoromethoxy)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidin-5-yl)-3-(4-fluorophenyl)acrylamide

    [0329] ##STR00098##

    [0330] P-toluenesulfonic acid monohydrate (1.52 g, 8 mmol) was added to a solution of 4-fluoro-2-trifluoromethoxy-5-nitroaniline (576 mg, 2.40 mmol) and compound 6-2 (814 mg, 2.00 mmol) in dioxane (20 mL). The reaction solution was heated to 100° C. and reacted for 16 hours. The reaction solution was cooled, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by column chromatography (dichloromethane:methanol:concentrated ammonia=100:0:0 to 97:3:0.3) to obtain compound 6-3 (722 mg, yield: 59%).

    [0331] MS m/z (ESI): 611.1[M+H].sup.+.

    Step 4: Trans-N-(2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-5-nitro-2-(trifluoromethoxy)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidin-5-yl)-3-(4-fluorophenyl)acrylamide

    [0332] ##STR00099##

    [0333] Potassium carbonate (138 mg, 1.0 mmol) was added to a solution of N,N,N′-trimethylethylenediamine (61 mg, 0.60 mmol) and compound 6-3 (305 mg, 0.50 mmol) in acetonitrile (10 mL). The reaction solution was heated to 80° C. and reacted for 2 hours. The reaction solution was cooled, and concentrated to dryness. The resulting residues were dissolved in dichloromethane (20 mL), and washed with brine (15 mL*3). The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by column chromatography (dichloromethane:methanol:concentrated ammonia=100:0:0 to 95:5:0.5) to obtain compound 6-4 (267 mg, yield: 77%).

    [0334] MS m/z (ESI): 693.5[M+H].sup.+.

    Step 5: Trans-N-(2-((2-(dimethylamino)ethyl)(methyl)amino)-5-((5-(3-(4-fluorophenyl)acrylamido)-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-(trifluoromethoxy)phenyl)but-2-ynamide

    [0335] ##STR00100## ##STR00101##

    [0336] Zinc powder (37 mg, 0.57 mmol) was added to a solution of compound 6-4 (100 mg, 0.14 mmol) and ammonium chloride (77 mg, 1.4 mmol) in acetone (5 mL) and water (1 mL). The reaction solution was stirred at room temperature for 40 minutes. The reaction solution was filtered. Dichloromethane (10 mL) was added to the organic phase, and washed with water (5 mL*2). The solid was rinsed with dichloromethane (10 mL). The organic phases were combined, dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude intermediate 6-5. The crude intermediate 6-5 was dissolved in dichloromethane (5 mL), followed by the successive addition of 2-butynoic acid (17 mg, 0.20 mmol), N,N-diisopropylethylamine (37 mg, 0.29 mmol) and 2-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (76 mg, 0.20 mmol). The reaction solution was reacted at room temperature for 16 hours. The reaction solution was concentrated under reduced pressure, and the resulting residues were purified by preparative HPLC to obtain compound 6 (34.2 mg, yield: 32%).

    [0337] MS m/z (ESI): 729.3[M+H].sup.+.

    Example 7

    N-(6-((4-(1-Methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)quinolin-4-yl)acrylamide

    [0338] ##STR00102##

    Step 1: 4-Chloro-N-(4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)quinolin-6-amine (Intermediate 7-1)

    [0339] ##STR00103##

    [0340] P-toluenesulfonic acid monohydrate (5.70 g, 30 mmol) was added to a solution of 4-chloroquinolin-6-amine (1.78 g, 10 mmol) and 3-(2-chloropyrimidin-4-yl)-1-methyl-1H-indole (2.43 g, 10 mmol) in dioxane (80 mL). The reaction solution was heated to 100° C. and reacted for 16 hours. The reaction solution was cooled, and concentrated to dryness to obtain the crude product. The resulting crude product was purified by column chromatography (dichloromethane:methanol:concentrated ammonia=100:0:0 to 97:3:0.3) to obtain compound 7-1 (1.73 g, yield: 45%).

    [0341] MS m/z (ESI): 386.1[M+H].sup.+.

    Step 2: N.SUP.6.-(4-(1-Methyl-1H-indol-3-yl)pyrimidin-2-yl)quinoline-4,6-diamine (Intermediate 7-2)

    [0342] ##STR00104##

    [0343] A mixture of intermediate 7-1 (1.00 g, 2.6 mmol) and phenol (10 g) was heated to 150° C. Ammonia gas was introduced and reacted for 2 hours. The reaction solution was cooled, to which water (100 mL) was added. 6M sodium hydroxide solution was added to adjust the pH to above 11, and the solution was extracted with dichloromethane (50 mL*3). The organic phases were combined, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The resulting residues were purified by column chromatography (dichloromethane:methanol:concentrated ammonia=100:0:0 to 90:10:1) to obtain compound 7-3 (433 mg, yield: 46%).

    [0344] MS m/z (ESI): 367.1[M+H].sup.+.

    Step 3: N-(6-((4-(1-Methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)quinolin-4-yl)acrylamide (compound 7)

    [0345] ##STR00105##

    [0346] Intermediate 7-2 (100 mg, 0.27 mmol) and triethylamine (50 mg, 0.5 mmol) were dissolved in dichloromethane (5 mL), followed by the addition of acryloyl chloride (30 mg, 0.33 mmol) at room temperature. The reaction solution was stirred at room temperature for 2 hours. The reaction solution was concentrated, and the resulting residues were purified by preparative HPLC to obtain compound 7 (21 mg, yield: 18%).

    [0347] MS m/z (ESI): 421.2[M+H].sup.+.

    Example 8

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(dimethylphosphono)pyrimidin-2-yl)amino)-4-methoxy-2-(9-methyl-3,9-diazaspiro[5.5]undec-3-yl)phenyl)acrylamide

    [0348] ##STR00106##

    [0349] The compound of Example 8 was prepared by referring to the method of Example 2.

    [0350] MS m/z (ESI): 678.2 [M+H].sup.+.

    Example 9

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(dimethylphosphoryl)pyrimidin-2-yl)amino)-4-methoxy-2-(9-methyl-3,9-diazaspiro[5.5]undecan-3-yl)phenyl)acrylamide

    [0351] ##STR00107##

    [0352] The compound of Example 9 was prepared by referring to the method of Example 2.

    [0353] MS m/z (ESI): 668.3 [M+H].sup.+.

    Example 10

    N-(5-((5-Chloro-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)but-2-ynamide

    [0354] ##STR00108##

    [0355] The compound of Example 10 was prepared by referring to the method of Example 1.

    [0356] MS m/z (ESI): 572.3 [M+H].sup.+.

    Example 11

    N-(5-((5-Azido-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(9-methyl-3,9-diazaspiro[5.5]undecan-3-yl)phenyl)acrylamide

    [0357] ##STR00109##

    [0358] The compound of Example 11 was prepared by referring to the method of Example 3.

    [0359] MS m/z (ESI): 607.1 [M+H].sup.+.

    Example 12

    Isopropyl 2-((5-acrylamido-2-methoxy-4-(methyl(quinuclidin-3-yl)amino)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0360] ##STR00110##

    [0361] The compound of Example 12 was prepared by referring to the method of Example 4.

    [0362] MS m/z (ESI): 624.1 [M+H].sup.+.

    Example 13

    Isopropyl (E)-2-((3-(3-(1-cyclopropylpyrrolidin-2-yl)acrylamido)quinoxalin-6-yl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0363] ##STR00111##

    [0364] The compound of Example 13 was prepared by referring to the method of Example 7.

    [0365] MS m/z (ESI): 617.3 [M+H].sup.+.

    Example 14

    2-((2-((1-Acryloylpiperidin-4-yl)oxy)quinolin-7-yl)amino)-4-(1-methyl-1H-indazol-3-yl)pyrimidine-5-carbonitrile

    [0366] ##STR00112##

    [0367] The compound of Example 14 was prepared by referring to the method of Example 7.

    [0368] MS m/z (ESI): 531.2 [M+H].sup.+.

    Example 15

    Isopropyl (E)-2-((3-(4-(dimethylamino)but-2-enamido)-2-methylquinolin-6-yl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0369] ##STR00113##

    [0370] The compound of Example 15 was prepared by referring to the method of Example 7.

    [0371] MS m/z (ESI): 578.1 [M+H].sup.+.

    Example 16

    Isopropyl 2-((5-acrylamido-4-(5-amino-5-methylhexahydrocyclopenta[c]pyrrol-2(1H)-yl)-2-methoxyphenyl)amino)-4-(1-cyclopropyl-1H-indol-pyridinyl-3-yl)pyrimidine-5-carboxylate

    [0372] ##STR00114##

    [0373] The compound of Example 16 was prepared by referring to the method of Example 4.

    [0374] MS m/z (ESI): 650.2 [M+H].sup.+.

    Example 17

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(thiazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(8-methyloctahydro-2H-pyrazino[1,2-a]pyrazin-2-yl)phenyl)acrylamide

    [0375] ##STR00115##

    [0376] The compound of Example 17 was prepared by referring to the method of Example 3.

    [0377] MS m/z (ESI): 662.2 [M+H].sup.+.

    Example 18

    N-(2-(5-Amino-5-methylhexahydrocyclopenta[c]pyrrol-2(1H)-yl)-5-((4-(1-cyclopropyl-1H-indol-3-yl)-5-(1H-pyrazol-1-yl)pyrimidin-2-yl)amino)-4-methoxyphenyl)acrylamide

    [0378] ##STR00116##

    [0379] The compound of Example 18 was prepared by referring to the methods of Examples 3 and 4.

    [0380] MS m/z (ESI): 630.1 [M+H].sup.+.

    Example 19

    Isopropyl 2-((5-acrylamido-4-((3aR,6aS)-hexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-2-methoxyphenyl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0381] ##STR00117##

    [0382] The compound of Example 19 was prepared by referring to the method of Example 2.

    [0383] MS m/z (ESI): 622.3 [M+H].sup.+.

    Example 20

    N-(5-((5-(Dimethylphosphoryl)-4-(1-(4-fluorobenzyl)-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-ethoxy-2-(9-methyl-3,9-diazaspiro[5.5]undecan-3-yl)phenyl)acrylamide

    [0384] ##STR00118##

    [0385] The compound of Example 20 was prepared by referring to the method of Example 3.

    [0386] MS m/z (ESI): 750.4 [M+H].sup.+.

    Example 21

    (E)-N-(5-((5-(3-(4-Cyanophenyl)ureido)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-cyclopropoxyphenyl)-3-(1-methylpyrrolidin-2-yl)acrylamide

    [0387] ##STR00119##

    [0388] The compound of Example 21 was prepared by referring to the method of Example 5.

    [0389] MS m/z (ESI): 694.1 [M+H].sup.+.

    Example 22

    (E)-N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-((4-(trifluoromethoxy)benzyl)oxy)pyrimidin-2-yl)amino)-2-methylphenyl)-3-(1-methylpyrrolidin-2-yl)acrylamide

    [0390] ##STR00120##

    [0391] The compound of Example 22 was prepared by referring to the method of Example 5.

    [0392] MS m/z (ESI): 683.3 [M+H].sup.+.

    Example 23

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(prop-1-yn-1-yl)pyrimidin-2-yl)amino)-2-((1S,4S)-5-cyclopropyl-2,5-diazabicyclo[2.2.1]heptan-2-yl)-4-methoxyphenyl)acrylamide

    [0393] ##STR00121##

    [0394] The compound of Example 23 was prepared by referring to the method of Example 3.

    [0395] MS m/z (ESI): 600.2 [M+H].sup.+.

    Example 24

    Isopropyl 2-((5-acrylamido-2-methoxy-4-(5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0396] ##STR00122##

    [0397] The compound of Example 24 was prepared by referring to the method of Example 1.

    [0398] MS m/z (ESI): 610.3[M+H].sup.+.

    [0399] .sup.1H NMR (400 MHz, DMSO-d6) δ 8.66-8.58 (m, 2H), 8.24 (s, 1H), 8.08 (s, 1H), 7.78 (br, 1H), 7.52-7.46 (m, 1H), 7.25-7.15 (m, 1H), 7.15-7.02 (m, 1H), 6.78 (s, 1H), 6.66 (br, 1H), 6.39-6.19 (m, 1H), 5.83-5.76 (m, 1H), 5.12-4.91 (m, 1H), 3.87 (s, 3H), 3.82 (s, 3H), 3.15-3.01 (m, 9H), 2.81-2.70 (m, 5H), 1.22-0.98 (m, 6H).

    Example 25

    Methyl 2-((5-acrylamido-2-methoxy-4-((1S,4S)-5-methyl-2,5-diazabicyclo[2.2.1]heptan-2-yl)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0400] ##STR00123##

    [0401] The compound of Example 25 was prepared by referring to the method of Example 2, and the synthetic route is as follows:

    ##STR00124##

    [0402] MS m/z (ESI): 568.1 [M+H].sup.+.

    [0403] .sup.1H NMR (400 MHz, Methanol-d4) δ 8.74 (s, 1H), 8.68-8.31 (m, 3H), 7.99 (s, 1H), 7.86-7.71 (m, 1H), 7.51-7.38 (m, 1H), 7.28-7.18 (m, 1H), 7.18-7.05 (m, 1H), 6.61 (s, 1H), 6.54-6.41 (m, 1H), 6.41-6.27 (m, 1H), 5.86-5.73 (m, 1H), 4.48-4.32 (m, 1H), 4.29-4.16 (m, 1H), 3.96 (s, 3H), 3.89 (s, 3H), 3.82-3.66 (m, 4H), 3.66-3.55 (m, 1H), 3.38-3.34 (m, 1H), 3.27-3.21 (m, 1H), 2.90 (s, 3H), 2.35-2.19 (m, 2H).

    Example 26

    Isopropyl 2-((5-acrylamido-2-methoxy-4-(6-methyl-2,6-diazaspiro[3.3]heptan-2-yl)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0404] ##STR00125##

    [0405] The compound of Example 26 was prepared by referring to the method of Example 2.

    [0406] MS m/z (ESI): 596.4 [M+H].sup.+.

    [0407] .sup.1H NMR (400 MHz, Methanol-d4) δ 8.69 (s, 1H), 8.40-8.20 (m, 1H), 8.00-7.83 (m, 1H), 7.83-7.70 (m, 1H), 7.50-7.36 (m, 1H), 7.30-7.17 (m, 1H), 7.18-7.04 (m, 1H), 6.59-6.18 (m, 3H)), 5.86-5.71 (m, 1H), 5.55-5.41 (m, 1H), 5.10-4.98 (m, 1H), 4.68-4.63 (m, 1H), 4.46-4.40 (m, 1H), 4.20-4.02 (m, 3H), 4.00-3.76 (m, 6H), 3.70-3.54 (m, 2H), 3.12 (s, 3H), 2.55-2.40 (m, 2H), 1.22-0.98 (m, 6H).

    Example 27

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(trifluoromethyl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0408] ##STR00126##

    [0409] The compound of Example 27 was prepared by referring to the method of Example 2.

    [0410] MS m/z (ESI): 618.2 [M+H].sup.+.

    Example 28

    N-(5-((5-Bromo-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0411] ##STR00127##

    [0412] The compound of Example 28 was prepared by referring to the method of Example 2, and the synthetic route is as follows:

    ##STR00128## ##STR00129##

    Step 1: Preparation of 3-(5-bromo-2-chloropyrimidin-4-yl)-1-cyclopropyl-1H-indole

    [0413] ##STR00130##

    [0414] 5-Bromo-2,4-dichloropyrimidine (1.2 g, 6.3 mmol) was dissolved in 1,2-dichloroethane (10 mL), and the solution was cooled to 0° C. Ferric chloride (1.7 g, 10.6 mmol) was added, and the reaction solution was stirred at room temperature for half an hour. 1-Cyclopropyl-1H-indole (0.83 g, 5.3 mmol) was added, and the reaction solution was stirred at 60° C. for 1 hour. Water (30 mL) was added, and the solution was filtered. The filtrate was extracted with dichloromethane (20 mL×3). The organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by column chromatography (petroleum ether/ethyl acetate: 100/1˜dichloromethane/ethyl acetate: 5/1) to obtain 3-(5-bromo-2-chloropyrimidin-4-yl)-1-cyclopropyl-1H-indole (0.7 g, yield: 38%) as a brown solid.

    Step 2: Preparation of 5-bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(4-fluoro-2-methoxy-5-nitrophenyl)pyrimidin-2-amine

    [0415] ##STR00131##

    [0416] 3-(5-Bromo-2-chloropyrimidin-4-yl)-1-cyclopropyl-1H-indole (0.4 g, 1.2 mmol), 4-fluoro-2-methoxy-5-nitroaniline (0.32 g, 1.7 mmol) and p-toluenesulfonic acid (1.14 g, 6.0 mmol) were dissolved in dioxane (10 mL). The reaction solution was stirred at 100° C. overnight. The reaction solution was cooled to room temperature, to which water (10 mL) was added. The solution was extracted with dichloromethane (10 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by column chromatography (dichloromethane/ethyl acetate: 100/1˜3/1) to obtain 5-bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(4-fluoro-2-methoxy-5-nitrophenyl)pyrimidin-2-amine (0.3 g, 53%) as a yellow solid.

    [0417] MS m/z (ESI): 498.1 [M+H].sup.+.

    Step 3: Preparation of 5-bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-nitrophenyl)pyrimidin-2-amine

    [0418] ##STR00132##

    [0419] 5-Bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(4-fluoro-2-methoxy-5-nitrophenyl)pyri midin-2-amine (100 mg, 0.2 mmol) was dissolved in acetonitrile (10 mL), followed by the addition of potassium carbonate (83 mg, 0.6 mmol) and (3aR,6aS)-2-methyloctahydropyrrolo[3,4-c]pyrrole (30 mg, 0.24 mmol). The reaction solution was stirred at 80° C. for 1 hour. The reaction solution was cooled to room temperature and filtered. The filtrate was concentrated to dryness by rotary evaporation to obtain the crude 5-bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((3aR,6aS)-5-methylhexahydro pyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-nitrophenyl)pyrimidin-2-amine (100 mg, yield: 83%) as a red gel.

    [0420] MS m/z (ESI): 604.1 [M+H].sup.+.

    Step 4: Preparation of N1-(5-bromo-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)-6-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzene-1,3-diamine

    [0421] ##STR00133##

    [0422] 5-Bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((3aR,6aS)-5-methylhexa hydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-nitrophenyl)pyrimidin-2-amine (100 mg, 0.17 mmol) was dissolved in ethanol (5 mL), followed by the addition of saturated ammonium chloride solution (2.5 mL) and iron powder (46 mg, 0.83 mmol). The reaction solution was stirred at 70° C. for 1 hour. The reaction solution was cooled to room temperature and filtered. The filtrate was extracted with dichloromethane (20 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain N1-(5-bromo-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)-6-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzene-1,3-diamine (100 mg, yield: 100% crude) as a yellow solid.

    [0423] MS m/z (ESI): 474.1 [M+H].sup.+.

    Step 5: Preparation of N-(5-((5-bromo-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0424] ##STR00134##

    [0425] N1-(5-Bromo-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)-6-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzene-1,3-diamine (30 mg, 0.05 mmol) was dissolved in tetrahydrofuran (1 mL), and the solution was cooled to 0° C. Triethylamine (10.6 mg, 0.1 mmol) and 3-chloropropionyl chloride (6.6 mg, 0.05 mmol) were added successively. The reaction solution was stirred at 0° C. for 1 hour. Water (5 mL) was added, and the solution was extracted with dichloromethane (5 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was dissolved in acetonitrile (3 mL), followed by the addition of sodium hydroxide (18 mg, 0.5 mmol) in water (0.5 mL). The reaction solution was stirred at room temperature for 1 hour. Water (5 mL) was added, and the solution was extracted with dichloromethane (10 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by prep-TLC to obtain N-(5-((5-bromo-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide (5.4 mg, yield: 19%) as a pale yellow solid.

    [0426] MS m/z (ESI): 628.1 [M+H].sup.+.

    Example 29

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0427] ##STR00135##

    [0428] The compound of Example 29 was prepared by referring to the method of Example 2.

    [0429] MS m/z (ESI): 550.3 [M+H].sup.+.

    [0430] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.60 (br, 1H), 9.03-8.83 (br, 1H), 8.50 (s, 1H), 8.40-8.37 (m, 1H), 8.14-8.11 (m, 1H), 7.65-7.60 (m, 2H), 7.35-7.28 (m, 2H), 7.16 (d, J=5.2 Hz, 1H), 6.76 (s, 1H), 6.45-6.37 (m, 2H), 5.76-5.70 (m, 1H), 3.88 (s, 3H), 3.48-3.40 (m, 1H), 3.05-2.81 (m, 8H), 2.68-2.55 (m, 2H), 2.48 (s, 3H), 1.25-1.15 (m, 4H).

    Example 30

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(trifluoromethyl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)but-2-ynamide

    [0431] ##STR00136##

    [0432] The compound of Example 30 was prepared by referring to the method of Example 1.

    [0433] MS m/z (ESI): 630.4 [M+H].sup.+.

    [0434] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.19-9.08 (br, 1H), 8.72 (s, 1H), 8.54-8.40 (br, 1H), 8.24 (d, J=8.0 Hz, 1H), 7.74-7.70 (m, 2H), 7.60 (d, J=8.0 Hz, 1H), 7.32-7.24 (m, 2H), 6.74 (s, 1H), 3.85 (s, 3H), 3.47-3.42 (m, 1H), 3.08-2.86 (m, 8H), 2.56-2.48 (m, 2H), 2.47 (s, 3H), 2.01 (s, 3H), 1.15-1.03 (m, 4H).

    Example 31

    2-((5-Acrylamido-2-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)amino)-N,N-dimethyl-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxamide

    [0435] ##STR00137##

    [0436] The compound of Example 31 was prepared by referring to the method of Example 5 with intermediate 1-2 as the starting material, and the specific synthetic route is as follows:

    ##STR00138##

    [0437] MS m/z (ESI): 595.2 [M+H].sup.+.

    [0438] .sup.1H NMR (400 MHz, DMSO-d6) δ 9.18-9.07 (m, 1H), 8.45-8.35 (m, 1H), 8.28-8.20 (m, 1H), 8.21-8.11 (m, 1H), 8.11-8.00 (m, 1H), 7.90-7.80 (m, 1H), 7.47-7.35 (m, 1H), 7.20-7.07 (m, 1H), 7.07-6.93 (m, 1H), 6.70 (s, 1H), 6.57-6.39 (m, 1H), 6.19-6.03 (m, 1H), 5.73-5.55 (m, 1H), 3.89-3.64 (m, 6H), 3.45-3.38 (m, 4H), 3.20-3.13 (m, 8H), 2.86-2.66 (m, 4H), 2.20 (s, 3H).

    Example 32

    2-((5-Acrylamido-2-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)amino)-N-cyclopropyl-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxamide

    [0439] ##STR00139##

    [0440] The compound of Example 32 was prepared by referring to the method of Example 5.

    [0441] MS m/z (ESI): 607.1 [M+H].sup.+.

    [0442] .sup.1H NMR (400 MHz, DMSO-d6) δ 9.18-9.07 (m, 1H), 8.45-8.35 (m, 1H), 8.28-8.20 (m, 1H), 8.21-8.11 (m, 2H), 8.11-8.00 (m, 1H), 7.90-7.80 (m, 1H), 7.47-7.35 (m, 1H), 7.20-7.07 (m, 1H), 7.07-6.93 (m, 1H), 6.70 (s, 1H), 6.57-6.39 (m, 1H), 6.19-6.03 (m, 1H), 5.73-5.55 (m, 1H), 3.89-3.64 (m, 6H), 3.45-3.38 (m, 5H), 3.18-3.06 (m, 2H), 2.86-2.66 (m, 4H), 2.20 (s, 3H), 0.70-0.47 (m, 2H), 0.47-0.24 (m, 2H).

    Example 33

    2-((5-Acrylamido-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-N-benzyl-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxamide

    [0443] ##STR00140##

    [0444] The compound of Example 33 was prepared by referring to the method of Example 5.

    [0445] MS m/z (ESI): 633.3 [M+H].sup.+.

    Example 34

    2-((5-Acrylamido-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-N-(1-benzyl-1H-pyrazol-4-yl)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxamide

    [0446] ##STR00141##

    [0447] The compound of Example 34 was prepared by referring to the method of Example 5.

    [0448] MS m/z (ESI): 699.1 [M+H].sup.+.

    [0449] .sup.1H NMR (400 MHz, DMSO-d6) δ 10.55-10.42 (m, 1H), 10.18-10.06 (m, 1H), 8.81-8.67 (m, 1H), 8.54-8.43 (m, 1H), 8.43-8.34 (m, 1H), 8.19-7.99 (m, 2H), 8.00-7.90 (m, 1H), 7.53-7.40 (m, 2H), 7.40-7.26 (m, 3H), 7.27-7.19 (m, 2H), 7.21-7.12 (m, 1H), 7.11-7.01 (m, 1H), 7.01-6.91 (m, 1H), 6.48-6.34 (m, 1H), 6.31-6.12 (m, 1H), 5.88-5.67 (m, 1H), 5.31 (s, 2H), 3.99-3.62 (m, 6H), 2.98-2.82 (m, 2H), 2.74 (s, 3H), 2.42-2.27 (m, 2H), 2.29-2.02 (m, 6H).

    Example 35

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0450] ##STR00142##

    [0451] The compound of Example 35 was prepared by referring to the method of Example 2, and the synthetic route is as follows:

    ##STR00143##

    Step 1: Preparation of 2-chloro-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile

    [0452] ##STR00144##

    [0453] 2,4-Dichloropyrimidine-5-carbonitrile (2 g, 12 mmol) was dissolved in dichloroethane (30 mL), and the solution was cooled to 0° C. Ferric chloride (3.9 g, 24 mmol) was added, and the reaction solution was stirred at room temperature for half an hour. 1-Cyclopropyl-1H-indole (2.17 g, 14 mmol) was added, and the reaction solution was stirred at 60° C. for 2 hours. Water (30 mL) was added, and the solution was filtered. The filtrate was extracted with dichloromethane (30 mL×2). The organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by column chromatography (petroleum ether/ethyl acetate: 100/1˜3/1) to obtain 2-chloro-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (1.6 g, yield: 47%) as a brown solid.

    Step 2: Preparation of 4-(1-cyclopropyl-1H-indol-3-yl)-2-((4-fluoro-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile

    [0454] ##STR00145##

    [0455] 2-Chloro-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (1 g, 3.4 mmol), 4-fluoro-2-methoxy-5-nitroaniline (0.7 g, 3.7 mmol) and p-toluenesulfonic acid (0.7 g, 3.7 mmol) were dissolved in 2-pentanol (40 mL). The reaction solution was stirred at 100° C. overnight. The reaction solution was cooled to room temperature, to which water (50 mL) was added. The solution was extracted with dichloromethane (50 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by column chromatography (petroleum ether/ethyl acetate: 100/1˜1/1˜dichloromethane/ethyl acetate: 100/1˜10/1) to obtain 4-(1-cyclopropyl-1H-indol-3-yl)-2-((4-fluoro-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (1 g, 67%) as a brown solid.

    [0456] Steps 3 to 5 of Example 35 were carried out by referring to Steps 3 to 5 of Example 28.

    [0457] MS m/z (ESI): 575.2 [M+H].sup.+.

    [0458] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.20-9.05 (m, 1H), 8.88-8.73 (m, 1H), 8.64 (s, 1H), 8.56-8.41 (m, 2H), 7.73 (s, 1H), 7.66-7.58 (m, 1H), 7.34-7.28 (m, 1H), 6.74 (s, 1H), 6.46-6.30 (m, 2H), 5.76-5.69 (m, 1H), 3.88 (s, 3H), 3.52-3.41 (m, 1H), 3.05-2.90 (m, 6H), 2.88-2.59 (m, 5H), 2.56-2.44 (m, 3H), 1.19-1.09 (m, 4H).

    Example 36

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0459] ##STR00146##

    [0460] The compound of Example 36 was prepared by referring to the method of Example 3, and the synthetic route is as follows:

    ##STR00147##

    Step 1: Preparation of 4-(1-cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3, 4-c]pyrrol-2(1H)-yl)-5-nitrophenyl)-5-(oxazol-2-yl)pyrimidin-2-amine

    [0461] ##STR00148##

    [0462] 5-Bromo-4-(1-cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((3aR,6aS)-5-methylhexa hydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-nitrophenyl)pyrimidin-2-amine (200 mg, 0.33 mmol), 2-(tributylstannyl)oxazole (178 mg, 0.5 mmol) and 2-dicyclohexylphosphorus-2,4,6-triisopropylbiphenyl (157 mg, 0.33 mmol) were dissolved in N,N-dimethylformamide (5 mL), followed by the addition of tetrakis(triphenylphosphine)palladium (191 mg, 0.17 mmol). The reaction solution was purged with nitrogen, and stirred at 140° C. under microwave for 2 hours. The reaction solution was cooled to room temperature, to which water (20 mL) was added. The solution was extracted with dichloromethane (20 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by prep-TLC to obtain 4-(1-cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3, 4-c]pyrrol-2(1H)-yl)-5-nitrophenyl)-5-(oxazol-2-yl)pyrimidin-2-amine (200 mg, yield: 100% crude) as a yellow solid.

    [0463] MS m/z (ESI): 593.1 [M+H].sup.+.

    Step 2: Preparation of N1-(4-(1-cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)-6-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzene-1,3-diamine

    [0464] ##STR00149##

    [0465] 4-(1-Cyclopropyl-1H-indol-3-yl)-N-(2-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-5-nitrophenyl)-5-(oxazol-2-yl)pyrimidin-2-amine (200 mg, 0.34 mmol) was dissolved in ethanol (10 mL), followed by the addition of saturated ammonium chloride solution (5 mL) and iron powder (190 mg, 3.4 mmol). The reaction solution was stirred at 80° C. for 2 hours. The reaction solution was cooled to room temperature and filtered. The filtrate was extracted with dichloromethane (20 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain N1-(4-(1-cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)-6-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzene-1,3-diamine (200 mg, yield: 100% crude) as a yellow solid.

    [0466] MS m/z (ESI): 563.1 [M+H].sup.+.

    Step 3: Preparation of N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0467] ##STR00150##

    [0468] N1-(4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)-6-methoxy-4-((3 aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)benzene-1,3-diamine (200 mg, 0.36 mmol) was dissolved in tetrahydrofuran (10 mL), and the solution was cooled to 0° C. Triethylamine (54 mg, 0.53 mmol) and 3-chloropropionyl chloride (68 mg, 0.53 mmol) were added successively. The reaction solution was stirred at 0° C. for 1 hour. Sodium hydroxide (142 mg, 3.5 mmol) in water (1 mL) was added, and the reaction solution was stirred at room temperature for 1 hour. Water (10 mL) was added, and the solution was extracted with dichloromethane (10 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by prep-HPLC to obtain N-(5-((4-(1-cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide (15.3 mg, yield: 7%) as a pale yellow solid.

    [0469] MS m/z (ESI): 617.3 [M+H].sup.+.

    [0470] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.30-9.18 (m, 1H), 8.87-8.77 (m, 1H), 7.95-7.87 (m, 1H), 7.81-7.75 (m, 1H), 7.69-7.59 (m, 2H), 7.59-7.50 (m, 2H), 7.23-7.17 (m, 1H), 7.08 (s, 1H), 6.70 (s, 1H), 6.64-6.54 (m, 1H), 6.52-6.40 (m, 1H), 5.78-5.70 (m, 1H), 5.34-5.26 (m, 1H), 3.88 (s, 3H), 3.42 (s, 1H), 6.70 (s, 1H), 6.64 Ccy4H), 2.98-2.90 (m, 2H), 2.75-2.66 (m, 2H), 2.59-2.53 (m, 1H), 2.36-2.31 (m, 1H), 1.70-1.61 (m, 3H), 1.13-1.08 (m, 2H), 0.95-0.93 (m, 2H).

    Example 37

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0471] ##STR00151##

    [0472] The compound of Example 37 was prepared by referring to the method of Example 3, and the synthetic route is as follows:

    ##STR00152## ##STR00153##

    [0473] MS m/z (ESI): 593.1 [M+H].sup.+.

    [0474] .sup.1H NMR (400 MHz, CDCl.sub.3) δ9.65 (s, 1H), 8.86 (s, 1H), 8.14-7.98 (m, 1H), 7.76 (s, 1H), 7.57-7.44 (m, 2H), 7.21-7.12 (m, 2H), 7.02-6.94 (m, 1H), 6.73 (s, 1H), 6.47 (d, J=17.2 Hz, 1H), 5.73 (d, J=11.7 Hz, 1H), 5.40-5.22 (m, 1H), 3.88 (s, 3H), 3.43-3.36 (m, 1H), 3.22-3.04 (m, 2H), 2.88 (s, 2H), 2.73 (s, 3H), 2.70-2.35 (m, 6H), 2.25-2.18 (m, 1H), 2.05-1.96 (m, 1H), 1.15-1.01 (m, 4H).

    Example 38

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0475] ##STR00154##

    [0476] The compound of Example 38 was prepared by referring to the method of Example 3.

    [0477] MS m/z (ESI): 619.2[M+H].sup.+.

    [0478] .sup.1H NMR (400 MHz, CDCl.sub.3) δ9.75 (s, 1H), 8.86 (s, 1H), 7.79-7.65 (m, 2H), 7.55-7.45 (m, 2H), 7.21-7.12 (m, 2H), 7.02-6.93 (m, 1H), 6.72 (s, 1H), 6.49 (d, J=15.9 Hz, 1H), 5.78-5.71 (m, 1H), 5.34-5.22 (m, 1H), 3.88 (s, 3H), 3.44-3.35 (m, 1H), 3.32-3.03 (m, 4H), 2.75 (s, 3H), 2.56 (s, 3H), 2.25-2.19 (m, 1H), 2.05-1.98 (m, 1H), 1.37-1.29 (m, 5H), 1.16-1.02 (m, 4H).

    Example 39

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(8-methyloctahydro-2H-pyrazino[1,2-a]pyrazin-2-yl)phenyl)acrylamide

    [0479] ##STR00155##

    [0480] The compound of Example 39 was prepared by referring to the method of Example 3.

    [0481] MS m/z (ESI): 646.1[M+H].sup.+.

    [0482] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.63 (s, 1H), 8.86 (s, 1H), 8.55-8.46 (m, 1H), 7.77 (s, 1H), 7.73-7.63 (m, 2H), 7.55-7.53 (m, 1H), 7.50-7.48 (m, 1H), 7.21-7.13 (m, 2H), 7.02-6.94 (m, 1H), 6.74 (s, 1H), 6.47-6.31 (m, 2H), 5.80 (d, J=9.5 Hz, 1H), 5.37-5.24 (m, 1H), 3.88 (s, 3H), 3.46-3.39 (m, 1H), 3.09-2.78 (m, 8H), 2.71-2.51 (m, 5H), 2.26-2.19 (m, 1H), 2.04-1.98 (m, 1H), 1.16-1.02 (m, 4H).

    Example 40

    Cis N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(1-methylhexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl)phenyl)acrylamide

    [0483] ##STR00156##

    [0484] The compound of Example 40 was prepared by referring to the method of Example 3.

    [0485] MS m/z (ESI): 617.3[M+H].sup.+.

    [0486] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.33-9.19 (m, 2H), 8.82 (s, 1H), 8.06-7.86 (br, 1H), 7.72 (s, 1H), 7.54-7.50 (m, 2H), 7.42-7.32 (br, 1H), 7.19-7.14 (m, 2H), 7.03-7.00 (m, 1H), 6.66-6.51 (m, 2H), 6.48-6.43 (m, 1H), 5.71 (d, J=11.6 Hz, 1H), 3.86 (s, 3H), 3.42-3.22 (m, 3H), 3.16-3.04 (br, 1H), 2.98-2.91 (m, 2H), 2.90-2.81 (m, 2H), 2.76-2.66 (br, 1H), 2.62-2.49 (br, 3H), 2.32-2.29 (br, 1H), 2.28-2.17 (m, 1H), 1.11-1.05 (m, 4H).

    Example 41

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(3,5-dimethylisoxazol-4-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phen yl)acrylamide

    [0487] ##STR00157##

    [0488] The compound of Example 41 was prepared by referring to the method of Example 3.

    [0489] MS m/z (ESI): 645.2[M+H].sup.+.

    [0490] .sup.1H NMR (400 MHz, CDCl.sub.3) δ8.96 (s, 1H), 8.37-8.28 (m, 1H), 8.19 (s, 1H), 7.66-7.50 (m, 2H), 6.99 (s, 1H), 6.68 (s, 1H), 6.38 (d, J=16.6 Hz, 1H), 5.69 (d, J=10.3 Hz, 1H), 5.34-5.26 (m, 1H), 3.90 (s, 3H), 3.34-3.08 (m, 6H), 3.00-2.91 (m, 2H), 2.85-2.73 (m, 3H), 2.30-2.17 (m, 3H), 2.06-1.71 (m, 9H), 1.10-1.01 (m, 2H), 0.91-0.84 (m, 2H).

    Example 42

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1H-pyrazol-1-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0491] ##STR00158##

    [0492] The compound of Example 42 was prepared by referring to the method of Example 3.

    [0493] MS m/z (ESI): 616.4[M+H].sup.+.

    Example 43

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phen yl)acrylamide

    [0494] ##STR00159##

    [0495] The compound of Example 43 was prepared by referring to the method of Example 3.

    [0496] MS m/z (ESI): 630.3[M+H].sup.+.

    [0497] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.41 (s, 1H), 8.81 (s, 1H), 8.41 (s, 1H), 7.70 (d, J=7.2 Hz, 1H), 7.56-7.52 (m, 3H), 7.45 (s, 1H), 7.20-7.11 (m, 2H), 7.09-7.05 (m, 1H), 6.73 (s, 1H), 6.45-6.32 (m, 2H), 5.71 (d, J=11.2 Hz, 1H), 3.86 (s, 3H), 3.84 (s, 3H), 3.38-3.31 (m, 1H), 2.99-2.83 (m, 8H), 2.65-2.53 (br, 2H), 2.45 (s, 3H), 1.06-0.99 (m, 4H).

    Example 44

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(5-methyloxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0498] ##STR00160##

    [0499] The compound of Example 44 was prepared by referring to the method of Example 3.

    [0500] MS m/z (ESI): 631.1[M+H].sup.+.

    Example 45

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(thiazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0501] ##STR00161##

    [0502] The compound of Example 45 was prepared by referring to the method of Example 3.

    [0503] MS m/z (ESI): 633.1[M+H].sup.+.

    [0504] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.36-9.18 (br, 1H), 8.76 (s, 1H), 7.84-7.81 (m, 1H), 7.74-7.70 (m, 2H), 7.55-7.50 (m, 2H), 7.18-7.15 (m, 2H), 7.06-6.98 (m, 2H), 6.70 (s, 1H), 6.46-6.41 (m, 2H), 5.77-5.72 (m, 1H), 3.87 (s, 3H), 3.44-3.33 (m, 1H), 2.98-2.80 (m, 8H), 2.67-2.57 (br, 5H), 0.99-0.90 (m, 4H).

    Example 46

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(thiophen-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0505] ##STR00162##

    [0506] The compound of Example 46 was prepared by referring to the method of Example 3.

    [0507] MS m/z (ESI): 632.2[M+H].sup.+.

    Example 47

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-phenylpyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0508] ##STR00163##

    [0509] The compound of Example 47 was prepared by referring to the method of Example 3.

    [0510] MS m/z (ESI): 626.4[M+H].sup.+.

    Example 48

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(pyridin-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0511] ##STR00164##

    [0512] The compound of Example 48 was prepared by referring to the method of Example 3.

    [0513] MS m/z (ESI): 627.1[M+H].sup.+.

    Example 49

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(2,4-difluorophenoxy)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0514] ##STR00165##

    [0515] The compound of Example 49 was prepared by referring to the method of Example 3.

    [0516] MS m/z (ESI): 678.2[M+H].sup.+.

    Example 50

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(4-methylpiperazin-1-yl)phenyl)acrylamide

    [0517] ##STR00166##

    [0518] The compound of Example 50 was prepared by referring to the method of Example 3.

    [0519] MS m/z (ESI): 591.1[M+H].sup.+.

    [0520] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.59 (s, 1H), 8.85 (s, 1H), 8.42-8.35 (br, 1H), 8.08-7.94 (br, 1H), 7.81 (s, 1H), 7.54 (d, J=8.0 Hz, 1H), 7.51 (s, 1H), 7.40-7.31 (br, 1H), 7.20-7.15 (m, 2H), 7.04-6.94 (m, 1H), 6.81 (s, 1H), 6.42 (d, J=6.0 Hz, 1H), 6.34-6.27 (m, 1H), 5.77 (d, J=8.8 Hz, 1H), 3.89 (s, 3H), 3.43-3.37 (m, 1H), 3.22-2.80 (m, 8H), 2.62 (s, 3H), 1.13-1.05 (m, 4H).

    Example 51

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(4-(4-methylpiperazin-1-yl)piperidin-1-yl)phenyl)acrylamide

    [0521] ##STR00167##

    [0522] The compound of Example 51 was prepared by referring to the method of Example 3.

    [0523] MS m/z (ESI): 674.2[M+H].sup.+.

    [0524] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.60 (s, 1H), 8.85 (s, 1H), 8.52 (s, 1H), 8.11-8.02 (br, 1H), 7.76 (s, 1H), 7.53 (d, J=8.4 Hz, 1H), 7.49 (s, 1H), 7.30-7.28 (m, 1H), 7.19 (s, 1H), 7.15 (t, J=7.6 Hz, 1H), 7.00-6.94 (m, 1H), 6.73 (s, 1H), 6.44-6.40 (m, 1H), 6.34-6.27 (m, 1H), 5.77 (d, J=10.0 Hz, 1H), 3.88 (s, 3H), 3.44-3.36 (m, 1H), 3.08-2.70 (m, 11H), 2.52-2.48 (m, 4H), 2.10-2.06 (m, 2H), 1.65-1.41 (m, 3H), 1.15-1.05 (m, 4H).

    Example 52

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-2-(3-(dimethylamino)azetidin-1-yl)-4-methoxyphenyl)acrylamide

    [0525] ##STR00168##

    [0526] The compound of Example 52 was prepared by referring to the method of Example 3.

    [0527] MS m/z (ESI): 591.2[M+H].sup.+.

    [0528] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 8.86 (s, 1H), 8.76 (s, 1H), 7.70-7.64 (m, 3H), 7.58-7.51 (m, 2H), 7.41-7.28 (m, 2H), 7.18-7.22 (m, 2H), 7.08-7.04 (m, 1H), 6.35-6.23 (m, 2H), 5.74 (d, J=10.0 Hz, 1H), 3.96-3.88 (m, 5H), 3.74-3.67 (m, 2H), 3.42-3.33 (m, 1H), 3.23-3.17 (m, 1H), 2.27 (s, 6H), 1.05-0.97 (s, 4H).

    Example 53

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(3-(pyrrolidin-1-yl)azetidin-1-yl)phenyl)acrylamide

    [0529] ##STR00169##

    [0530] The compound of Example 53 was prepared by referring to the method of Example 3.

    [0531] MS m/z (ESI): 617.3[M+H].sup.+.

    [0532] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 8.86-8.66 (m, 2H), 7.70-7.51 (m, 5H), 7.36-7.29 (m, 1H), 7.23-7.18 (m, 1H), 7.10-7.02 (m, 1H), 6.44-6.28 (m, 3H), 5.73 (d, J=10.7 Hz, 1H), 3.94-3.87 (m, 5H), 3.80-3.72 (m, 2H), 3.40-3.33 (m, 2H), 2.63-2.50 (m, 5H), 1.88-1.82 (m, 4H), 1.11-1.01 (m, 4H).

    Example 54

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(4-(pyrrolidin-1-yl)piperidin-1-yl)phenyl)acrylamide

    [0533] ##STR00170##

    [0534] The compound of Example 54 was prepared by referring to the method of Example 3.

    [0535] MS m/z (ESI): 645.4[M+H].sup.+.

    [0536] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.59 (s, 1H), 8.85 (s, 1H), 8.55 (s, 1H), 8.11 (s, 1H), 7.75 (s, 1H), 7.54 (d, J=8.3 Hz, 1H), 7.48 (s, 1H), 7.20-7.12 (m, 2H), 7.00-6.93 (m, 1H), 6.72 (s, 1H), 6.45-6.41 (m, 1H), 5.78 (d, J=8.7 Hz, 1H), 3.87 (s, 3H), 3.44-3.37 (m, 1H), 3.16-3.06 (m, 2H), 2.80-2.65 (m, 3H), 2.22-2.01 (m, 6H), 1.45-1.19 (m, 5H), 1.17-1.03 (m, 4H), 0.95-0.89 (m, 1H).

    Example 55

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-2-((3aR, 6aS)-5-cyclopropylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-4-methoxyphenyl)acrylamide

    [0537] ##STR00171##

    [0538] The compound of Example 55 was prepared by referring to the method of Example 3.

    [0539] MS m/z (ESI): 643.2[M+H].sup.+.

    Example 56

    (S)—N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-2-(3-(dimethylamino)pyrrolidin-1-yl)-4-methoxyphenyl)acrylamide

    [0540] ##STR00172##

    [0541] The compound of Example 56 was prepared by referring to the method of Example 3.

    [0542] MS m/z (ESI): 605.1[M+H].sup.+.

    [0543] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.39 (s, 1H), 8.83 (s, 1H), 8.69-8.51 (m, 1H), 8.09-7.84 (m, 1H), 7.73 (s, 1H), 7.58-7.47 (m, 2H), 7.44-7.32 (m, 1H), 7.23-7.13 (m, 2H), 7.08-6.94 (m, 1H), 6.82-6.61 (m, 2H), 6.43 (d, J=16.9 Hz, 1H), 5.73 (d, J=10.6 Hz, 1H), 3.87 (s, 3H), 3.44-3.28 (m, 3H), 3.23-2.99 (m, 3H), 2.55 (s, 6H), 2.32-2.16 (m, 2H), 1.17-1.01 (m, 4H).

    Example 57

    (R)—N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-2-(3-(dimethylamino)pyrrolidin-1-yl)-4-methoxyphenyl)acrylamide

    [0544] ##STR00173##

    [0545] The compound of Example 57 was prepared by referring to the method of Example 3.

    [0546] MS m/z (ESI): 605.3[M+H].sup.+.

    [0547] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.39 (s, 1H), 8.83 (s, 1H), 8.66-8.51 (m, 1H), 8.06-7.87 (m, 1H), 7.73 (s, 1H), 7.64-7.47 (m, 2H), 7.37 (mi, 1H), 7.22-7.10 (m, 2H), 7.05-6.96 (m, 1H), 6.80-6.60 (m, 2H), 6.43 (d, J=17.0 Hz, 1H), 5.73 (d, J=9.8 Hz, 1H), 3.87 (s, 3H), 3.47-3.24 (m, 3H), 3.23-2.98 (m, 3H), 2.52 (s, 6H), 2.29-2.19 (m, 2H), 1.17-0.97 (m, 4H).

    Example 58

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-2-(4-(dimethylamino)piperidin-1-yl)-4-methoxyphenyl)acrylamide

    [0548] ##STR00174##

    [0549] The compound of Example 58 was prepared by referring to the method of Example 3.

    [0550] MS m/z (ESI): 619.1[M+H].sup.+.

    Example 59

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(4-morpholinopiperidin-1-yl)phenyl)acrylamide

    [0551] ##STR00175##

    [0552] The compound of Example 59 was prepared by referring to the method of Example 3.

    [0553] MS m/z (ESI): 661.1[M+H].sup.+.

    Example 60

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-2-(4-cyclopropylpiperazin-1-yl)-4-methoxyphenyl)acrylamide

    [0554] ##STR00176##

    [0555] The compound of Example 60 was prepared by referring to the method of Example 3.

    [0556] MS m/z (ESI): 617.2[M+H].sup.+.

    Example 61

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(methylsulfonyl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0557] ##STR00177##

    [0558] The compound of Example 61 was prepared by referring to the method of Example 3.

    [0559] MS m/z (ESI): 628.3[M+H].sup.+.

    Example 62

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(dimethylphosphoryl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0560] ##STR00178##

    [0561] The compound of Example 62 was prepared by referring to the method of Example 3.

    [0562] MS m/z (ESI): 626.2[M+H].sup.+.

    Example 63

    Isopropyl 2-((5-acrylamido-2-methoxy-4-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxylate

    [0563] ##STR00179##

    [0564] The compound of Example 63 was prepared by referring to the method of Example 2.

    [0565] MS m/z (ESI): 610.2 [M+H].sup.+.

    [0566] 1H NMR (400 MHz, DMSO-d6) δ 8.66-8.58 (m, 2H), 8.24 (s, 1H), 8.08 (s, 1H), 7.78 (br, 1H), 7.49 (d, J=8.2 Hz, 1H), 7.20 (t, J=7.6 Hz, 1H), 7.15-7.02 (m, 1H), 6.78 (s, 1H), 6.66 (br, 1H), 6.39-6.19 (m, 1H), 5.83-5.76 (m, 1H), 5.12-4.91 (m, 1H), 3.87 (s, 3H), 3.82 (s, 3H), 3.15-3.01 (m, 9H), 2.76 (s, 5H), 1.13 (d, J=6.2 Hz, 6H).

    Example 64

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(pyridin-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0567] ##STR00180##

    [0568] The compound of Example 64 was prepared by referring to the method of Example 3.

    [0569] MS m/z (ESI): 627.2 [M+H].sup.+.

    [0570] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.39 (br, 1H), 8.94-8.73 (br, 1H), 8.60 (d, J=1.6 Hz, 1H), 8.51 (d, J=4.0 Hz, 1H), 8.41 (s, 1H), 7.78-7.60 (m, 3H), 7.49 (d, J=8.0 Hz, 1H), 7.23-7.15 (m, 2H), 7.05-7.02 (m, 1H), 7.09-7.05 (m, 1H), 6.75 (s, 1H), 6.45-6.35 (m, 2H), 5.74-5.72 (m, 1H), 3.88 (s, 3H), 3.33-3.25 (m, 1H), 2.96-2.86 (m, 8H), 2.65-2.50 (br, 2H), 2.48 (s, 3H), 0.99-0.90 (m, 4H).

    Example 65

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aS,6aS)-1-methylhexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl)phen yl)acrylamide

    [0571] ##STR00181##

    [0572] The compound of Example 65 was prepared by referring to the method of Example 3.

    [0573] MS m/z (ESI): 630.3 [M+H].sup.+.

    [0574] .sup.1H NMR (400 MHz, Chloroform-d) δ 9.24-9.06 (br, 1H), 8.42-8.26 (m, 1H), 7.85-7.64 (m, 1H), 7.60-7.49 (m, 2H), 7.49-7.35 (m, 2H), 7.24-7.16 (m, 2H), 7.15-7.06 (m, 1H), 6.93-6.68 (br, 1H), 6.63 (s, 1H), 6.49-6.35 (m, 1H), 5.74-5.59 (m, 1H), 3.93-3.76 (m, 7H), 3.53-3.40 (m, 1H), 3.38-3.25 (m, 2H), 3.08-2.98 (m, 1H), 2.98-2.88 (m, 1H), 2.88-2.76 (m, 3H), 2.69 (s, 3H), 2.60-2.44 (m, 1H), 2.38-2.25 (m, 1H), 2.05-1.91 (m, 1H), 1.09-0.93 (m, 4H).

    Example 66

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)-2-(3-(dimethylamino)azetidin-1-yl)-4-methoxyphenyl)-2-fluoroacrylamide

    [0575] ##STR00182##

    [0576] The compound of Example 66 was prepared by referring to the method of Example 3.

    [0577] MS m/z (ESI): 609.2[M+H].sup.+.

    [0578] .sup.1H NMR (400 MHz, CDCl.sub.3) MR (400 MHz, br, 1H), 8.76 (s, 1H), 7.84-7.81 (m, 1H), 7.74-7.70 (m, 2H), 7.55-7.50 (m, 2H), 7.18-7.15 (m, 2H), 7.06-6.98 (m, 2H), 6.70 (s, 1H), 6.46-6.41 (m, 2H), 3.87 (s, 3H), 3.44-3.33 (m, 1H), 2.98-2.80 (m, 8H), 2.67-2.57 (br, 3H), 0.99-0.90 (m, 4H).

    Example 67

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethyl amino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0579] ##STR00183##

    [0580] The compound of Example 67 was prepared by referring to the method of Example 2, and the specific synthetic route is as follows:

    ##STR00184##

    Step 1: Preparation of 4-(1-cyclopropyl-1H-indol-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile

    [0581] ##STR00185##

    [0582] 4-(1-Cyclopropyl-1H-indol-3-yl)-2-((4-fluoro-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (0.4 g, 0.9 mmol) was dissolved in acetonitrile (20 mL), followed by the addition of potassium carbonate (0.37 g, 2.7 mmol) and N1,N1,N2-trimethylethane-1,2-diamine (0.11 g, 1.1 mmol). The reaction solution was stirred at 80° C. for 1 hour. The reaction solution was cooled to room temperature and filtered. The filtrate was concentrated to dryness by rotary evaporation to obtain the crude product 4-(1-cyclopropyl-1H-indol-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (0.48 g, yield: 100% crude) as a yellow solid.

    Step 2: Preparation of 2-((5-amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile

    [0583] ##STR00186##

    [0584] 4-(1-Cyclopropyl-1H-indol-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-m ethoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (0.48 g, 0.97 mmol) was dissolved in ethanol (20 mL), followed by the addition of saturated sodium bicarbonate solution (10 mL) and iron powder (0.54 g, 9.7 mmol). The reaction solution was stirred at 80° C. for 2 hours. The reaction solution was cooled to room temperature and filtered. The filtrate was extracted with dichloromethane (20 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain 2-((5-amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.4 g, yield: 88%) as a yellow solid.

    Step 3: Preparation of N-(5-((5-cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0585] ##STR00187##

    [0586] 2-((5-Amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.4 g, 0.8 mmol) was dissolved in tetrahydrofuran (10 mL), and the solution was cooled to 0° C. Triethylamine (0.12 g, 1.2 mmol) and 3-chloropropionyl chloride (0.12 g, 0.97 mmol) were added successively. The reaction solution was stirred at 0° C. for 1 hour. 3M sodium hydroxide solution (3 mL) was added, and the reaction solution was stirred at room temperature for 1 hour. Water (30 mL) was added, and the solution was extracted with dichloromethane (30 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by prep-TLC (dichloromethane/MeOH: 20/1) to obtain N-(5-((5-cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide (0.15 g, yield: 34%) as a yellow solid.

    [0587] MS m/z (ESI): 551.4 [M+H].sup.+.

    [0588] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.08 (s, 1H), 9.42 (s, 1H), 8.68 (s, 1H), 8.49-8.47 (m, 2H), 7.76 (s, 1H), 7.61 (d, J=8.0 Hz, 1H), 7.30-7.26 (m, 2H), 6.81 (s, 1H), 6.39 (s, 2H), 5.69-5.67 (m, 1H), 3.89 (s, 3H), 3.48-3.44 (m, 1H), 2.93-2.91 (m, 2H), 2.73 (s, 3H), 2.35-2.30 (m, 8H), 1.17-1.11 (m, 4H).

    Example 68

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aR)-1-hexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl)phenyl)-2-fluoroacrylamide

    [0589] ##STR00188##

    [0590] The compound of Example 68 was prepared by referring to the method of Example 3.

    [0591] MS m/z (ESI): 630.4[M+H].sup.+.

    [0592] .sup.1H NMR (400 MHz, Chloroform-d) δ 9.39 (s, 1H), 9.07-8.84 (m, 1H), 8.40 (s, 1H), 7.69 (d, J=7.9 Hz, 1H), 7.60-7.41 (m, 4H), 7.23-7.14 (m, 2H), 7.06 (t, J=7.5 Hz, 1H), 6.68 (s, 1H), 6.54-6.28 (m, 2H), 5.79-5.60 (m, 1H), 3.85 (s, 3H), 3.83 (s, 3H), 3.41-3.28 (m, 1H), 3.20-3.02 (m, 2H), 3.00-2.86 (m, 3H), 2.86-2.72 (m, 1H), 2.70-2.56 (m, 1H), 2.44 (s, 3H), 2.38-2.25 (m, 1H), 2.24-2.07 (m, 1H), 1.83-1.61 (m, 1H), 1.12-0.87 (m, 4H).

    Example 69

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(2,4-difluorophenyl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0593] ##STR00189##

    [0594] The compound of Example 69 was prepared by referring to the method of Example 3.

    [0595] MS m/z (ESI): 662.0[M+H].sup.+.

    [0596] .sup.1H NMR (400 MHz, Chloroform-d) δ 9.37 (s, 1H), 8.75 (br s, 1H), 8.35 (s, 1H), 7.97 (s, 1H), 7.60 (s, 1H), 7.49 (d, J=8.1 Hz, 1H), 7.24-7.17 (m, 2H), 7.14-6.98 (m, 2H), 6.88-6.83 (m, 2H), 6.74 (s, 1H), 6.43-6.39 (m, 2H), 5.77-5.65 (m, 1H), 3.88 (s, 3H), 3.29-3.26 (m, 1H), 2.96-2.86 (m, 8H), 2.70-2.54 (m, 2H), 2.47 (s, 3H), 1.01-0.96 (m, 2H), 0.89-0.78 (m, 2H).

    Example 70

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-5-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0597] ##STR00190##

    [0598] The compound of Example 70 was prepared by referring to the method of Example 3.

    [0599] MS m/z (ESI): 630.2[M+H].sup.+.

    [0600] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.11 (s, 1H), 8.53-8.47 (m, 1H), 8.31 (s, 1H), 7.66-7.59 (m, 2H), 7.56-7.49 (m, 1H), 7.35-7.27 (m, 2H), 6.73 (s, 1H), 6.44-6.34 (m, 3H), 5.70 (d, J=11.8 Hz, 1H), 3.91 (s, 3H), 3.40 (s, 3H), 3.32-3.22 (m, 2H), 3.21-2.89 (m, 9H), 2.76-2.60 (m, 3H), 1.03-0.97 (m, 2H), 0.83-0.77 (m, 2H).

    Example 71

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aS)-5-methylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)phenyl)acrylamide

    [0601] ##STR00191##

    [0602] The compound of Example 71 was prepared by referring to the method of Example 3.

    [0603] MS m/z (ESI): 630.1[M+H].sup.+.

    [0604] .sup.1H NMR (400 MHz, Chloroform-d) δ 9.26 (s, 1H), 8.94-8.71 (m, 1H), 8.62 (s, 1H), 7.71-7.58 (m, 2H), 7.51 (d, J=8.5 Hz, 1H), 7.39-7.32 (m, 1H), 7.23 (s, 1H), 7.19-7.12 (m, 1H), 7.07-6.98 (m, 1H), 6.67 (s, 1H), 6.48-6.30 (m, 2H), 5.97 (s, 1H), 5.73-5.70 (m, 1H), 3.94 (s, 3H), 3.86 (s, 3H), 3.43-3.27 (m, 2H), 3.11-2.65 (m, 10H), 2.57-2.49 (m, 2H), 1.08-0.92 (m, 4H).

    Example 72

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0605] ##STR00192##

    [0606] The compound of Example 72 was prepared by referring to the method of Example 2.

    [0607] MS m/z (ESI): 577.1 [M+H].sup.+.

    Example 73

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl(2-(pyrrolidin-1-yl)ethyl)amino)phenyl)acrylamide

    [0608] ##STR00193##

    [0609] The compound of Example 73 was prepared by referring to the method of Example 2.

    [0610] MS m/z (ESI): 577.4 [M+H].sup.+.

    Example 74

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-(3-(dimethylamino)azetidin-1-yl)-4-methoxyphenyl)acrylamide

    [0611] ##STR00194##

    [0612] The compound of Example 74 was prepared by referring to the method of Example 2.

    [0613] MS m/z (ESI): 549.2 [M+H].sup.+.

    [0614] .sup.1H NMR (400 MHz, Chloroform-d) δ 8.90-8.72 (m, 1H), 8.59 (s, 1H), 8.54-8.36 (m, 2H), 7.76 (s, 1H), 7.67-7.56 (m, 1H), 7.39-7.27 (m, 3H), 6.86-6.58 (m, 1H), 6.56-6.29 (m, 2H), 5.81-5.62 (m, 1H), 4.42-4.22 (m, 2H), 4.10-3.99 (m, 2H), 3.96 (s, 3H), 3.86-3.76 (m, 1H), 3.56-3.39 (m, 1H), 2.81 (s, 6H), 1.27-1.20 (m, 4H).

    Example 75

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(3-(pyrrolidin-1-yl)azetidin-1-yl)phenyl)acrylamide

    [0615] ##STR00195##

    [0616] The compound of Example 75 was prepared by referring to the method of Example 2.

    [0617] MS m/z (ESI): 575.1 [M+H].sup.+.

    Example 76

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-(2-((dimethyl amino)methyl)azetidin-1-yl)-4-methoxyphenyl)acrylamide

    [0618] ##STR00196##

    [0619] The compound of Example 76 was prepared by referring to the method of Example 2.

    [0620] MS m/z (ESI): 563.5 [M+H].sup.+.

    Example 77

    (S)—N-(5-((5-cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-(3-(dimethylamino)pyrrolidin-1-yl)-4-methoxyphenyl)acrylamide

    [0621] ##STR00197##

    [0622] The compound of Example 77 was prepared by referring to the method of Example 2.

    [0623] MS m/z (ESI): 563.3 [M+H].sup.+.

    [0624] .sup.1H NMR (400 MHz, Chloroform-d) δ 9.08 (s, 1H), 8.73-8.59 (m, 1H), 8.56-8.39 (m, 2H), 8.14 (br s, 1H), 7.71 (s, 1H), 7.62 (d, J=8.1 Hz, 1H), 7.34-7.20 (m, 2H), 6.72 (s, 1H), 6.46-6.28 (m, 2H), 5.78-5.66 (m, 1H), 3.89 (s, 3H), 3.49-3.44 (m, 1H), 3.24-3.15 (m, 4H), 2.97-2.89 (m, 1H), 2.35 (s, 6H), 2.24-2.16 (m, 1H), 2.04-1.96 (m, 1H), 1.22-1.05 (m, 4H).

    Example 78

    (R)—N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-(3-(dimethylamino)pyrrolidin-1-yl)-4-methoxyphenyl)acrylamide

    [0625] ##STR00198##

    [0626] The compound of Example 78 was prepared by referring to the method of Example 2.

    [0627] MS m/z (ESI): 563.3 [M+H].sup.+.

    [0628] .sup.1H NMR (400 MHz, Chloroform-d) δ 9.08 (s, 1H), 8.63 (s, 1H), 8.55-8.46 (m, 2H), 8.24-8.11 (m, 1H), 7.71 (s, 1H), 7.62 (d, J=8.1 Hz, 1H), 7.37-7.27 (m, 2H), 6.71 (s, 1H), 6.37 (s, 2H), 5.79-5.66 (m, 1H), 3.89 (s, 3H), 3.50-3.44 (m, 1H), 3.26-3.09 (m, 4H), 2.99-2.89 (m, 1H), 2.37 (s, 6H), 2.25-2.17 (m, 1H), 2.05-1.97 (m, 1H), 1.18-1.07 (m, 4H).

    Example 79

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-(4-(3-(cyano methyl)azetidin-1-yl)piperidin-1-yl)-4-methoxyphenyl)acrylamide

    [0629] ##STR00199##

    [0630] The compound of Example 79 was prepared by referring to the method of Example 2.

    [0631] MS m/z (ESI): 628.2 [M+H].sup.+.

    Example 80

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(4-(3-(methoxymethyl)azetidin-1-yl)piperidin-1-yl)phenyl)acrylamide

    [0632] ##STR00200##

    [0633] The compound of Example 80 was prepared by referring to the method of Example 2.

    [0634] MS m/z (ESI): 633.3 [M+H].sup.+.

    Example 81

    N-(2-((2-(Dimethylamino)ethyl)(methyl)amino)-4-methoxy-5-((4-(1-methyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0635] ##STR00201##

    [0636] The compound of Example 81 was prepared by referring to the method of Example 3.

    [0637] MS m/z (ESI): 567.1 [M+H].sup.+.

    [0638] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.16-9.96 (br, 1H), 9.78 (s, 1H), 8.86 (s, 1H), 8.77-8.56 (br, 1H), 7.88 (s, 1H), 7.47 (s, 1H), 7.28 (d, J=8.0 Hz, 1H), 7.19 (s, 1H), 7.13 (t, J=8.0 Hz, 1H), 7.01-6.88 (m, 2H), 6.78 (s, 1H), 6.45 (d, J=13.2 Hz, 2H), 5.73-6.69 (m, 1H), 3.94 (s, 3H), 3.88 (s, 3H), 3.00-2.87 (br, 2H), 2.71 (s, 3H), 2.42-2.21 (br, 8H).

    Example 82

    N-(4-Methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)-5-((4-(1-methyl-1H-indol-3-yl)-5-(oxazol-2-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0639] ##STR00202##

    [0640] The compound of Example 82 was prepared by referring to the method of Example 3.

    [0641] MS m/z (ESI): 593.4 [M+H].sup.+.

    Example 83

    N-(2-((2-(Dimethylamino)ethyl)(methyl)amino)-4-methoxy-5-((4-(1-methyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0642] ##STR00203##

    [0643] The compound of Example 83 was prepare by referring to the method of Example 3.

    [0644] MS m/z (ESI): 580.5 [M+H].sup.+.

    Example 84

    N-(4-Methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)-5-((4-(1-methyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0645] ##STR00204##

    [0646] The compound of Example 84 was prepared by referring to the method of Example 3.

    [0647] MS m/z (ESI): 606.2 [M+H].sup.+.

    Example 85

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0648] ##STR00205##

    [0649] The compound of Example 85 was prepared by referring to the method of Example 3.

    [0650] MS m/z (ESI): 606.3 [M+H].sup.+.

    [0651] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.25-10.01 (br, 1H), 9.62 (s, 1H), 8.44 (s, 1H), 7.66-7.61 (m, 2H), 7.56 (s, 1H), 7.52 (d, J=8.0 Hz, 1H), 7.41 (s, 1H), 7.19-7.16 (m, 2H), 7.05 (t, J=7.2 Hz, 1H), 6.77 (s, 1H), 6.48-6.21 (m, 2H), 5.71-5.68 (m, 1H), 3.87 (s, 3H), 3.83 (s, 3H), 3.38-3.30 (m, 1H), 3.00-2.84 (m, 2H), 2.71 (s, 3H), 2.40-2.16 (m, 8H), 1.04-1.00 (m, 4H).

    Example 86

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0652] ##STR00206##

    [0653] The compound of Example 86 was prepared by referring to the method of Example 3.

    [0654] MS m/z (ESI): 632.1 [M+H].sup.+.

    Example 87

    N-(2-((2-(Dimethylamino)ethyl)(methyl)amino)-4-methoxy-5-((4-(1-methyl-1H-indol-3-yl)-5-(pyridin-3-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0655] ##STR00207##

    [0656] The compound of Example 87 was prepared by referring to the method of Example 3.

    [0657] MS m/z (ESI): 577.5 [M+H].sup.+.

    Example 88

    N-(4-Methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)-5-((4-(1-methyl-1H-indol-3-yl)-5-(pyridin-3-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0658] ##STR00208##

    [0659] The compound of Example 88 was prepared by referring to the method of Example 3.

    [0660] MS m/z (ESI): 603.2 [M+H].sup.+.

    Example 89

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(pyridin-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0661] ##STR00209##

    [0662] The compound of Example 89 was prepared by referring to the method of Example 3.

    [0663] MS m/z (ESI): 603.1[M+H].sup.+.

    Example 90

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(pyridin-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0664] ##STR00210##

    [0665] The compound of Example 90 was prepared by referring to the method of Example 3.

    [0666] MS m/z (ESI): 629.3 [M+H].sup.+.

    Example 91

    N-(2-((2-(Bis(methyl-d3)amino)ethyl)(methyl)amino)-5-((5-cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxyphenyl)acrylamide

    [0667] ##STR00211##

    [0668] The compound of Example 91 was prepared by referring to the method of Example 2.

    [0669] MS m/z (ESI): 557.1 [M+H].sup.+.

    Example 92

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-(methyl-d3)pyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0670] ##STR00212##

    [0671] The compound of Example 92 was prepared by referring to the method of Example 2.

    [0672] MS m/z (ESI): 580.4 [M+H].sup.+.

    Example 93

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethyl amino)ethyl)(methyl-d3)amino)-4-methoxyphenyl)acrylamide

    [0673] ##STR00213##

    [0674] The compound of Example 93 was prepared by referring to the method of Example 2.

    [0675] MS m/z (ESI): 554.2 [M+H].sup.+.

    Example 94

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((methyl-d3)((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0676] ##STR00214##

    [0677] The compound of Example 94 was prepared by referring to the method of Example 2.

    [0678] MS m/z (ESI): 580.5 [M+H].sup.+.

    Example 95

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-(methyl-d3)-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0679] ##STR00215##

    [0680] The compound of Example 95 was prepared by referring to the method of Example 3.

    [0681] MS m/z (ESI): 609.4 [M+H].sup.+.

    Example 96

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1-(methyl-d3)-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0682] ##STR00216##

    [0683] The compound of Example 96 was prepared by referring to the method of Example 3.

    [0684] MS m/z (ESI): 635.2 [M+H].sup.+.

    Example 97

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0685] ##STR00217##

    [0686] The compound of Example 97 was prepared by referring to the method of Example 3.

    [0687] MS m/z (ESI): 592.1 [M+H].sup.+.

    Example 98

    N-(2-((2-(Dimethylamino)ethyl)(methyl)amino)-4-methoxy-5-((5-(1-methyl-1H-pyrazol-4-yl)-4-(1-(methyl-d3)-1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0688] ##STR00218##

    [0689] The compound of Example 98 was prepared by referring to the method of Example 3.

    [0690] MS m/z (ESI): 583.3 [M+H].sup.+.

    Example 99

    N-(5-((4-(1H-Indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)-2-((2-(di methylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0691] ##STR00219##

    [0692] The compound of Example 99 was prepared by referring to the method of Example 3.

    [0693] MS m/z (ESI): 566.4 [M+H].sup.+.

    Example 100

    N-(4-Methoxy-2-(methyl(2-(methylamino)ethyl)amino)-5-((4-(1-methyl-1H-indol-3-yl)-5-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0694] ##STR00220##

    [0695] The compound of Example 100 was prepared by referring to the method of Example 3.

    [0696] MS m/z (ESI): 566.2 [M+H].sup.+.

    Example 101

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(isopropylsulfonyl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0697] ##STR00221##

    [0698] The compound of Example 101 was prepared by referring to the method of Example 2.

    [0699] MS m/z (ESI): 632.3[M+H].sup.+.

    Example 102

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(isopropylsulfonyl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0700] ##STR00222##

    [0701] The compound of Example 102 was prepared by referring to the method of Example 2.

    [0702] MS m/z (ESI): 658.1[M+H].sup.+.

    Example 103

    N-(2-((2-(Dimethylamino)ethyl)(methyl)amino)-4-methoxy-5-((4-(1-methyl-1H-indol-3-yl)-5-(methylsulfonyl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0703] ##STR00223##

    [0704] The compound of Example 103 was prepared by referring to the method of Example 2.

    [0705] MS m/z (ESI): 578.4[M+H].sup.+.

    Example 104

    N-(4-Methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)-5-((4-(1-methyl-1H-indol-3-yl)-5-(methylsulfonyl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0706] ##STR00224##

    [0707] The compound of Example 104 was prepared by referring to the method of Example 2.

    [0708] MS m/z (ESI): 604.5[M+H].sup.+.

    Example 105

    N-(2-((2-(Dimethylamino)ethyl)(methyl)amino)-5-((5-(dimethylphosphoryl)-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxyphenyl)acrylamide

    [0709] ##STR00225##

    [0710] The compound of Example 105 was prepared by referring to the method of Example 3.

    [0711] MS m/z (ESI): 576.1[M+H].sup.+.

    Example 106

    N-(5-((5-(Dimethylphosphoryl)-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0712] ##STR00226##

    [0713] The compound of Example 106 was prepared by referring to the method of Example 3.

    [0714] MS m/z (ESI): 602.2[M+H].sup.+.

    Example 107

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(dimethylphosphoryl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0715] ##STR00227##

    [0716] The compound of Example 107 was prepared by referring to the method of Example 3.

    [0717] MS m/z (ESI): 602.3[M+H].sup.+.

    Example 108

    N-(5-((4-(1-Cyclopropyl-1H-indol-3-yl)-5-(dimethylphosphoryl)pyrimidin-2-yl)amino)-4-methoxy-2-(methyl((1-methylpyrrolidin-2-yl)methyl)amino)phenyl)acrylamide

    [0718] ##STR00228##

    [0719] The compound of Example 108 was prepared by referring to the method of Example 3.

    [0720] MS m/z (ESI): 628.1[M+H].sup.+.

    Example 109

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aR,6aR)-1-methylhexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl)phenyl)acrylamide

    [0721] ##STR00229##

    [0722] The compound of Example 109 was prepared by referring to the method of Example 2.

    [0723] MS m/z (ESI): 575.2[M+H].sup.+.

    [0724] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.10 (br, 1H), 9.21-9.00 (br, 1H), 8.64 (s, 1H), 8.55-8.38 (m, 2H), 7.69 (br, 1H), 7.60 (d, J=8.0 Hz, 1H), 7.32-7.29 (m, 2H), 6.77 (s, 1H), 6.45-6.32 (m, 2H), 5.70-5.67 (m, 1H), 3.88 (s, 3H), 3.48-3.35 (m, 2H), 3.08-3.01 (m, 2H), 2.98-2.81 (m, 4H), 2.72-2.23 (m, 6H), 1.17-1.11 (m, 4H).

    Example 110

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-4-methoxy-2-((3aS,6aS)-1-methylhexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl)phenyl)acrylamide

    [0725] ##STR00230##

    [0726] The compound of Example 110 was prepared by referring to the method of Example 2.

    [0727] MS m/z (ESI): 575.3 [M+H].sup.+.

    [0728] .sup.1H NMR (400 MHz, Chloroform-d) δ 8.90-8.72 (m, 1H), 8.56 (s, 1H), 8.51-8.30 (m, 2H), 7.64 (s, 1H), 7.59-7.45 (m, 1H), 7.40-7.21 (m, 3H), 6.70-6.47 (m, 1H), 6.47-6.10 (m, 2H), 5.73-5.45 (m, 1H), 3.81 (s, 3H), 3.71-3.48 (m, 1H), 3.50-3.33 (m, 1H), 3.22-1.92 (m, 9H), 1.33-0.65 (m, 7H).

    Example 111

    N-(5-((5-Cyano-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0729] ##STR00231##

    Method 1

    [0730] ##STR00232## ##STR00233##

    Step 1: Preparation of 3-(5-bromo-2-chloropyrimidin-4-yl)-1H-indole

    [0731] ##STR00234##

    [0732] 2,4-Dichloro-5-bromopyrimidine (3.0 g, 13.17 mmol) was dissolved in 1,2-dichloroethane (50 mL), and the solution was cooled to 0° C. Aluminum trichloride (3.88 g, 23.94 mmol) was added, and the reaction solution was stirred at room temperature for half an hour. Indole (1.4 g, 11.97 mmol) was added, and the reaction solution was heated to 60° C. and reacted for 14 hours. The reaction solution was cooled to room temperature, neutralized with saturated sodium bicarbonate solution, and stirred at room temperature for 20 minutes. The solution was filtered, and the filter cake was rinsed with dichloromethane. The filter cake was dried by rotary evaporation to obtain the crude product 3-(5-bromo-2-chloropyrimidin-4-yl)-1H-indole (1.7 g, yield: 41.8%, yellow solid).

    [0733] MS m/z (ESI): 308.0, 310.0 [M+H].sup.+.

    Step 2: Preparation of 5-bromo-N-(4-fluoro-2-methoxy-5-nitrophenyl)-4-(1H-indol-3-yl)pyrimidin-2-amine

    [0734] ##STR00235##

    [0735] 3-(5-Bromo-2-chloropyrimidin-4-yl)-1H-indole (0.50 g, 1.62 mmol), 4-fluoro-2-methoxy-5-nitroaniline (0.33 g, 1.78 mmol) and p-toluenesulfonic acid (0.34 g, 1.78 mmol) were dissolved in 2-pentanol (25 mL). The reaction solution was reacted at 100° C. for 14 hours. The reaction solution was cooled to room temperature, neutralized with saturated sodium bicarbonate solution, and stirred at room temperature for 20 minutes. The solution was filtered, and the filter cake was rinsed with 2-pentanol followed by petroleum ether. The filter cake was dried to obtain 5-bromo-N-(4-fluoro-2-methoxy-5-nitrophenyl)-4-(1H-indol-3-yl)pyrimidin-2-amine (0.40 g, 54%, khaki solid).

    [0736] MS m/z (ESI): 458.0, 460.0 [M+H].sup.+.

    Step 3: Preparation of N.SUP.1.-(5-bromo-4-(1H-indol-3-yl)pyrimidin-2-yl)-N.SUP.4.-(2-(dimethylamino)ethyl)-2-methoxy-N.SUP.4.-methyl-5-nitrobenzene-1,4-diamine

    [0737] ##STR00236##

    [0738] 5-Bromo-N-(4-fluoro-2-methoxy-5-nitrophenyl)-4-(1H-indol-3-yl)pyrimidin-2-amine (0.40 g, 0.87 mmol) was dissolved in acetonitrile (20 mL), followed by the addition of potassium carbonate (0.36 g, 2.61 mmol) and N1,N1,N2-trimethylethane-1,2-diamine (0.10 g, 0.96 mmol). The reaction solution was stirred at 80° C. for 2 hours. The reaction solution was cooled to room temperature, diluted with ethyl acetate, and washed with saturated brine. The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain N.sup.1-(5-bromo-4-(1H-indol-3-yl)pyrimidin-2-yl)-N.sup.4-(2-(dimethylamino)ethyl)-2-methoxy-N.sup.4-methyl-5-nitrobenzene-1,4-diamine (0.40 g, yield: 87%, red solid).

    [0739] MS m/z (ESI): 540.2, 542.2 [M+H].sup.+.

    Step 4: Preparation of N.SUP.1.-(5-bromo-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)-N.SUP.4.-(2-(dimethylamino)ethyl)-2-methoxy-N.SUP.4.-methyl-5-nitrobenzene-1,4-diamine

    [0740] ##STR00237##

    [0741] N.sup.1-(5-Bromo-4-(1H-indol-3-yl)pyrimidin-2-yl)-N.sup.4-(2-(dimethylamino)ethyl)-2-methoxy-N.sup.4-methyl-5-nitrobenzene-1,4-diamine (0.30 g, 0.56 mmol), cesium carbonate (0.13 g, 0.72 mmol) and 3-iodooxetane (0.36 g, 1.11 mmol) were dissolved in N,N-dimethylformamide (8 mL) at room temperature. The reaction solution was reacted under microwave at 110° C. for 1.5 hours, followed by cooling to room temperature. The reaction solution was diluted with ethyl acetate and filtered. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by preparative thin layer chromatography (dichloromethane:methanol=20:1) to obtain N.sup.1-(5-bromo-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)-N.sup.4-(2-(dimethylamino)ethyl)-2-methoxy-N.sup.4-methyl-5-nitrobenzene-1,4-diamine (0.12 g, yield: 36%, red solid).

    [0742] MS m/z (ESI): 596.0, 598.0 [M+H].sup.+.

    Step 5: Preparation of 2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile

    [0743] ##STR00238##

    [0744] N.sup.1-(5-Bromo-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)-N.sup.4-(2-(dimethylamino)ethyl)-2-methoxy-N.sup.4-methyl-5-nitrobenzene-1,4-diamine (0.12 g, 0.20 mmol), Pd.sub.2(dba).sub.3 (18.4 mg, 0.02 mmol), X-Phos (19 mg, 0.04 mmol), zinc cyanide (23.5 mg, 0.20 mmol) and zinc powder (13 mg, 0.20 mmol) were dissolved in N,N-dimethylacetamide (3 mL) at room temperature. The reaction solution was purged with nitrogen, and reacted under microwave at 110° C. for 1.5 hours. The reaction solution was cooled to room temperature and filtered, and the resulting solid was rinsed with ethyl acetate. The organic phase was dried by an oil pump, and the resulting crude product was purified by preparative thin layer chromatography (dichloromethane:methanol=20:1) to obtain 2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.096 g, yield: 88%, yellow solid).

    [0745] MS m/z (ESI): 543.1 [M+H].sup.+.

    Step 6: Preparation of 2-((5-amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile

    [0746] ##STR00239##

    [0747] 2-((4-((2-(Dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.12 g, 0.22 mmol) was dissolved in ethanol (10 mL), followed by the addition of aqueous ammonium chloride solution (0.06 g, 1.1 mmol, water, 2 mL) and iron powder (0.12 g, 2.2 mmol). The reaction solution was stirred at 80° C. for 2 hours. The reaction solution was cooled to room temperature and filtered. The filter cake was rinsed with dichloromethane, and the organic solvent was removed under reduced pressure. The residues were dissolved in dichloromethane and water, and the resulting solution was partitioned. The aqueous phase was extracted with dichloromethane (20 mL×2). The organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain 2-((5-amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.095 g, yield: 83.8%, yellow oil).

    [0748] MS m/z (ESI): 513.2 [M+H].sup.+.

    Step 7: Preparation of N-(5-((5-cyano-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0749] ##STR00240##

    [0750] 2-((5-Amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.075 g, 0.146 mmol) was dissolved in dichloromethane (5 mL), and the solution was cooled to 0° C. Triethylamine (0.03 g, 0.292 mmol) and 3-chloropropionyl chloride (0.024 g, 0.19 mmol) were added successively. The reaction solution was stirred at 0° C. for 1 hour. After completion of the reaction, the reaction solution was concentrated to dryness by rotary evaporation. The resulting crude product was dissolved in dichloromethane, and washed with saturated sodium bicarbonate solution and saturated brine. The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was dissolved in acetonitrile (5 mL), followed by the addition of aqueous sodium hydroxide solution (0.058 g, 1.46 mmol, water, 0.5 mL). The reaction solution was reacted at 40° C. for two hours. The reaction solution was cooled to room temperature, diluted with ethyl acetate, and washed with saturated brine. The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by preparative chromatography to obtain N-(5-((5-cyano-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide (0.04 g, yield: 48%, yellow solid).

    Method 2

    [0751] ##STR00241##

    Step 1: Preparation of 3-(5-bromo-2-chloropyrimidin-4-yl)-1H-indole

    [0752] ##STR00242##

    [0753] Indole (5.0 g, 54.86 mmol) was dissolved in 2-methyltetrahydrofuran (50 mL) under an ice bath. Methyl magnesium bromide (3.0M 2-methyltetrahydrofuran solution, 18.3 mL, 54.86 mmol) was added dropwise, and the reaction solution was kept at below 30° C. After completion of the addition, the reaction solution was stirred at room temperature for 30 minutes. A solution of 2,4-dichloro-5-bromopyrimidine in 2-methyltetrahydrofuran (5.0 g, 21.94 mmol, 10 mL of solvent) was added dropwise. After completion of the addition, the reaction solution was stirred at room temperature for half an hour, and then heated to 70° C. for 14 hours. The reaction solution was cooled to room temperature, and poured into saturated aqueous ammonium chloride solution. A solid precipitated out, which was collected by filtration. The resulting solid was resuspended in water, sonicated and filtered. The resulting solid was dried to obtain the product 3-(5-bromo-2-chloropyrimidin-4-yl)-1H-indole (4.0 g, yield: 59%, yellow solid).

    [0754] MS m/z (ESI): 308.0 [M+H].sup.+.

    [0755] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 12.21 (s, 1H), 8.86 (s, 1H), 8.80 (s, 1H), 8.46 (d, J=7.2 Hz, 1H), 7.55 (d, J=7.2 Hz, 1H), 7.30-7.23 (m, 2H).

    Step 2: Preparation of 5-bromo-N-(4-fluoro-2-methoxy-5-nitrophenyl)-4-(1H-indol-3-yl)pyrimidin-2-amine

    [0756] ##STR00243##

    [0757] 3-(5-Bromo-2-chloropyrimidin-4-yl)-1H-indole (0.50 g, 1.62 mmol), 4-fluoro-2-methoxy-5-nitroaniline (0.33 g, 1.78 mmol) and p-toluenesulfonic acid (0.34 g, 1.78 mmol) were dissolved in 2-pentanol (25 mL). The reaction solution was reacted at 100° C. for 14 hours. The reaction solution was cooled to room temperature, neutralized with saturated sodium bicarbonate solution, and stirred at room temperature for 20 minutes. The solution was filtered, and the filter cake was rinsed with 2-pentanol followed by petroleum ether. The filter cake was dried to obtain 5-bromo-N-(4-fluoro-2-methoxy-5-nitrophenyl)-4-(1H-indol-3-yl)pyrimidin-2-amine (0.40 g, 54%, khaki solid).

    [0758] MS m/z (ESI): 458.0, 460.0 [M+H].sup.+.

    Step 3: Preparation of N1-(5-bromo-4-(1H-indol-3-yl)pyrimidin-2-yl)-N4-(2-(dimethylamino)ethyl)-2-methoxy-N4-methyl-5-nitrobenzene-1,4-diamine

    [0759] ##STR00244##

    [0760] 5-Bromo-N-(4-fluoro-2-methoxy-5-nitrophenyl)-4-(1H-indol-3-yl)pyrimidin-2-amine (0.40 g, 0.87 mmol) was dissolved in acetonitrile (20 mL), followed by the addition of potassium carbonate (0.36 g, 2.61 mmol) and N1,N1,N2-trimethylethane-1,2-diamine (0.10 g, 0.96 mmol). The reaction solution was stirred at 80° C. for 2 hours. The reaction solution was cooled to room temperature, diluted with ethyl acetate, and washed with saturated brine. The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain N1-(5-bromo-4-(1H-indol-3-yl)pyrimidin-2-yl)-N4-(2-(dimethylamino)ethyl)-2-methoxy-N4-methyl-5-nitrobenzene-1,4-diamine (0.40 g, yield: 87%, red solid).

    [0761] MS m/z (ESI): 540.2, 542.2 [M+H].sup.+.

    Step 4: Preparation of N1-(5-bromo-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)-N4-(2-(dimethylamino)ethyl)-2-methoxy-N4-methyl-5-nitrobenzene-1,4-diamine

    [0762] ##STR00245##

    [0763] N1-(5-Bromo-4-(1H-indol-3-yl)pyrimidin-2-yl)-N4-(2-(dimethylamino)ethyl)-2-meth oxy-N4-methyl-5-nitrobenzene-1,4-diamine (0.30 g, 0.56 mmol), cesium carbonate (0.13 g, 0.72 mmol) and 3-iodo-oxetane (0.36 g, 1.11 mmol) were dissolved in N,N-dimethylformamide (8 mL) at room temperature. The reaction solution was reacted under microwave at 110° C. for 1.5 hours, followed by cooling to room temperature. The reaction solution was diluted with ethyl acetate and filtered. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by silica gel preparative thin layer chromatography (dichloromethane:methanol=20:1) to obtain N1-(5-bromo-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)-N4-(2-(dimethylamino)eth yl)-2-methoxy-N4-methyl-5-nitrobenzene-1,4-diamine (0.12 g, yield: 36%, red solid).

    [0764] MS m/z (ESI): 596.0 [M+H].sup.+.

    Step 5: Preparation of 2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile

    [0765] ##STR00246##

    [0766] N1-(5-Bromo-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)-N4-(2-(dimethylamino)ethyl)-2-methoxy-N4-methyl-5-nitrobenzene-1,4-diamine (0.12 g, 0.20 mmol), Pd.sub.2(dba).sub.3 (18.4 mg, 0.02 mmol), X-Phos (19 mg, 0.04 mmol), zinc cyanide (23.5 mg, 0.20 mmol) and zinc powder (13 mg, 0.20 mmol) were dissolved in N,N-dimethylacetamide (3 mL) at room temperature. The reaction solution was purged with nitrogen, and reacted under microwave at 110° C. for 1.5 hours. The reaction solution was cooled to room temperature and filtered, and the resulting solid was rinsed with ethyl acetate. The organic phase was concentrated under reduced pressure, and the resulting crude product was purified by preparative thin layer chromatography (dichloromethane:methanol=20:1) to obtain 2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.096 g, yield: 88%, yellow solid).

    [0767] MS m/z (ESI): 543.1 [M+H].sup.+.

    Step 6: Preparation of 2-((5-amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile

    [0768] ##STR00247##

    [0769] 2-((4-((2-(Dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.12 g, 0.22 mmol) was dissolved in ethanol (10 mL), followed by the addition of aqueous ammonium chloride solution (0.06 g, 1.1 mmol, water, 2 mL) and iron powder (0.12 g, 2.2 mmol). The reaction solution was stirred at 80° C. for 2 hours. The reaction solution was cooled to room temperature and filtered. The filter cake was rinsed with dichloromethane, and the organic solvent was removed under reduced pressure. The residues were dissolved in dichloromethane and water, and the resulting solution was partitioned. The aqueous phase was extracted with dichloromethane (20 mL×2). The organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain 2-((5-amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.095 g, yield: 83.8%, yellow oil).

    [0770] MS m/z (ESI): 513.2 [M+H].sup.+.

    Step 7: Preparation of N-(5-((5-cyano-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0771] ##STR00248##

    [0772] 2-((5-Amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.075 g, 0.146 mmol) was dissolved in dichloromethane (5 mL), and the solution was cooled to 0° C. Triethylamine (0.03 g, 0.292 mmol) and 3-chloropropionyl chloride (0.024 g, 0.19 mmol) were added successively. The reaction solution was stirred at 0° C. for 1 hour. After completion of the reaction, the reaction solution was concentrated to dryness by rotary evaporation. The resulting crude product was dissolved in dichloromethane, and washed with saturated sodium bicarbonate solution and saturated brine. The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was dissolved in acetonitrile (5 mL), followed by the addition of aqueous sodium hydroxide solution (0.058 g, 1.46 mmol, water, 0.5 mL). The reaction solution was reacted at 40° C. for two hours. The reaction solution was cooled to room temperature, diluted with ethyl acetate, and washed with saturated brine. The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by preparative chromatography to obtain N-(5-((5-cyano-4-(1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide (0.04 g, yield: 48%, yellow solid).

    [0773] MS m/z (ESI): 567.2[M+H].sup.+.

    [0774] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.15-9.82 (br, 1H), 9.45 (s, 1H), 8.69 (s, 2H), 8.55-8.38 (br, 1H) 7.80 (s, 1H), 7.61 (d, J=8.0 Hz, 1H), 7.33-7.29 (m, 2H), 6.78 (s, 1H), 6.42-6.38 (m, 1H), 5.72-5.66 (m, 2H), 5.28-5.13 (m, 5H), 3.90 (s, 3H), 3.13-2.95 (br, 2H), 2.74 (s, 3H), 2.61-2.24 (br, 6H), 1.85-1.53 (m, 2H).

    Example 112

    N-(5-((5-Cyano-4-(1-cyclopropyl-6-methoxy-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0775] ##STR00249##

    [0776] The compound of Example 112 was prepared by referring to the method of Example 2.

    [0777] MS m/z (ESI): 581.4 [M+H].sup.+.

    [0778] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.04 (s, 1H), 9.38 (s, 1H), 8.66 (s, 1H), 8.40-8.38 (m, 2H), 7.71 (s, 1H), 7.06 (s, 1H), 6.92 (s, 1H), 6.77 (s, 1H), 6.42-6.38 (m, 1H), 5.71-5.68 (m, 1H), 3.91-3.89 (m, 6H), 3.41-3.40 (m, 1H), 3.10-2.90 (m, 3H), 2.74 (s, 3H), 2.70-2.30 (m, 8H), 1.16-1.10 (m, 4H).

    Example 113

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-(2-(dimethylamino)ethoxy)-4-methoxyphenyl)acrylamide

    [0779] ##STR00250##

    [0780] The compound of Example 113 was prepared by referring to the method of Example 2.

    [0781] MS m/z (ESI): 538.4[M+H].sup.+.

    [0782] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.76 (s, 1H), 9.26 (s, 1H), 8.64 (s, 1H), 8.47 (s, 1H), 7.77-7.63 (m, 1H), 7.61 (d, J=8.1 Hz, 1H), 7.34-7.27 (m, 1H), 6.65 (s, 1H), 6.35 (t, J=26.3 Hz, 2H), 5.69 (d, J=11.4 Hz, 1H), 4.16 (t, J=5.1 Hz, 2H), 3.87 (s, 3H), 3.52-3.39 (m, 1H), 2.74-2.59 (m, 2H), 2.40 (s, 6H), 1.21-1.04 (m, 4H).

    Example 114

    N-(5-((5-Cyano-4-(1-cyclopropyl-6-fluoro-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0783] ##STR00251##

    [0784] The compound of Example 114 was prepared by referring to the method of Example 2.

    [0785] MS m/z (ESI): 569.4 [M+H].sup.+.

    [0786] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.03-9.64 (m, 1H), 9.48-9.26 (m, 1H), 8.66 (s, 1H), 8.54-8.33 (m, 2H), 7.73 (s, 1H), 7.12-6.92 (m, 1H), 6.76 (s, 1H), 6.38 (d, J=17.0 Hz, 1H), 5.70 (d, J=11.4 Hz, 1H), 3.90 (s, 3H), 3.48-3.35 (m, 1H), 3.18-2.98 (m, 2H), 2.74 (s, 3H), 2.71-2.31 (m, 6H), 2.12-1.47 (m, 4H), 1.22-1.02 (m, 4H).

    Example 115

    N-(5-((5-Cyano-4-(6-cyano-1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0787] ##STR00252##

    [0788] The compound of Example 115 was prepared by referring to the method of Example 2.

    [0789] MS m/z (ESI): 576.4[M+H].sup.+.

    [0790] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 12.53 (s, 1H), 9.48 (s, 1H), 9.29 (s, 1H), 8.68 (s, 1H), 8.61 (s, 1H), 8.56-8.43 (m, 1H), 8.03-7.92 (m, 1H), 7.91-7.79 (m, 1H), 7.55-7.44 (m, 1H), 6.83-6.69 (m, 1H), 6.44-6.32 (m, 1H), 5.76 (d, J=10.2 Hz, 1H), 3.95 (s, 3H), 3.58-3.30 (m, 3H), 3.25-3.06 (m, 2H), 2.83 (s, 6H), 1.65 (s, 3H), 1.30-1.20 (m, 2H), 1.17-1.12 (s, 2H).

    Example 116

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethyl amino)ethyl)(methyl)amino)-6-methoxypyridin-3-yl)acrylamide

    [0791] ##STR00253##

    Method 1

    [0792] ##STR00254##

    Step 1: Preparation of 6-chloro-2-methoxypyridin-3-amine

    [0793] ##STR00255##

    [0794] 6-Chloro-2-methoxy-3-nitropyridine (5 g, 26.6 mmol) was dissolved in ethanol (100 mL) and water (30 mL). Ammonium chloride (7.0 g, 133 mmol) was added, and iron powder (7.5 g, 133 mmol) was added in batches. The reaction solution was stirred at 80° C. for 2 hours. The reaction solution was cooled to room temperature, and filtered through celite. Ethyl acetate (150 mL) and saturated brine (120 mL) were added to the filtrate, and the organic layer was separated. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain 6-chloro-2-methoxypyridin-3-amine (4 g, yield: 95%) as a brown solid.

    [0795] MS m/z (ESI): 159.1 [M+H].sup.+.

    Step 2: Preparation of N-(6-chloro-2-methoxypyridin-3-yl)acetamide

    [0796] ##STR00256##

    [0797] 6-Chloro-2-methoxypyridin-3-amine (2.0 g, 12.7 mmol) was dissolved in dichloromethane (100 mL), followed by the addition of diisopropylethylamine (3.5 mL, 19.0 mmol). The reaction solution was cooled to 0° C., to which acetyl chloride (1.2 g, 15.2 mmol) was added and stirred for 2 hours. The reaction solution was washed successively with 80 mL of water, 80 mL of 1N hydrochloric acid and 80 mL of saturated brine, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain N-(6-chloro-2-methoxypyridin-3-yl)acetamide (2.0 g, yield: 79%) as a brown solid.

    [0798] MS m/z (ESI): 201.1 [M+H].sup.+.

    Step 3: Preparation of N-(6-chloro-2-methoxy-5-nitropyridin-3-yl)acetamide

    [0799] ##STR00257##

    [0800] 6-Chloro-2-methoxypyridin-3-amine (2.0 g, 10.0 mmol) was dissolved in trifluoroacetic anhydride (20 mL), and cooled to −10° C. Fuming nitric acid (0.5 mL, 10 mmol) was added dropwise, and the reaction solution was stirred for 2 hours. Crushed ice was added to the reaction solution, and the solution was extracted with dichloromethane (50 mL). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain N-(6-chloro-2-methoxy-5-nitropyridin-3-yl)acetamide (1.6 g, yield: 65%) as a brown solid.

    [0801] MS m/z (ESI): 244.1 [M−H].sup.+.

    Step 4: Preparation of N-(6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropyridin-3-yl)acetamide

    [0802] ##STR00258##

    [0803] N-(6-chloro-2-methoxy-5-nitropyridin-3-yl)acetamide (1.6 g, 6.5 mmol) was dissolved in acetonitrile (30 mL), followed by the addition of N1,N1,N2-trimethylethane-1,2-diamine (1 g, 9.8 mmol). The reaction solution was stirred at 80° C. for 3 hours. The solvent was removed by rotary evaporation, and the resulting crude product was purified by column chromatography (dichloromethane/methanol: 100/1˜10/1) to obtain N-(6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropyridin-3-yl)acetamide (0.9 g, yield: 45%) as a brown solid.

    [0804] MS m/z (ESI): 312.1 [M+H].sup.+.

    Step 5: Preparation of N2-(2-(dimethylamino)ethyl)-6-methoxy-N2-methyl-3-nitropyridine-2,5-diamine

    [0805] ##STR00259##

    [0806] N-(6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropyridin-3-yl)acetamide (0.9 g, 2.9 mmol) was dissolved in methanol (30 mL) and concentrated hydrochloric acid (5 mL). The reaction solution was stirred at 60° C. for 3 hours. The solvent was removed by rotary evaporation, and dichloromethane (50 mL) and saturated sodium bicarbonate solution (50 mL) were added to the resulting residues and stirred until no bubbles generation. The organic layer was separated, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by column chromatography (dichloromethane/methanol: 100/1˜10/1) to obtain N2-(2-(dimethylamino)ethyl)-6-methoxy-N2-methyl-3-nitropyridine-2,5-diamine (0.15 g, yield: 19%) as a brown solid.

    [0807] MS m/z (ESI): 270.1 [M+H].sup.+.

    Step 6: Preparation of 4-(1-cyclopropyl-1H-indol-3-yl)-2-((6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropyridin-3-yl)amino)pyrimidine-5-carbonitrile

    [0808] ##STR00260##

    [0809] N2-(2-(Dimethylamino)ethyl)-6-methoxy-N2-methyl-3-nitropyridine-2,5-diamine (0.11 g, 0.41 mmol), 2-chloro-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.12 g, 0.41 mmol), tris(dibenzylideneacetone)dipalladium (0.18 g, 0.2 mmol), x-phos (0.2 g, 0.41 mmol) and sodium tert-butoxide (0.12 g, 1.2 mmol) were dissolved in dioxane (5 mL). The reaction solution was purged with nitrogen, and stirred under microwave at 140° C. for 1 hour. The reaction solution was cooled to room temperature and filtered. The filtrate was concentrated to dryness by rotary evaporation, and the resulting crude product was purified by prep-TLC (dichloromethane/methanol: 20/1) to obtain 4-(1-cyclopropyl-1H-indol-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (0.1 g, yield: 47%) as a yellow solid.

    [0810] MS m/z (ESI): 528.1 [M+H].sup.+.

    Step 7: Preparation of 2-((5-amino-6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxypyridin-3-yl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile

    [0811] ##STR00261##

    [0812] 4-(1-Cyclopropyl-1H-indol-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropphenyl)amino)pyrimidine-5-carbonitrile (0.1 g, 0.19 mmol) and platinum dioxide (0.05 g) were dissolved in tetrahydrofuran (5 mL). The reaction solution was purged with hydrogen, and stirred at room temperature overnight. The reaction solution was filtered, and the filtrate was concentrated to dryness by rotary evaporation to obtain 2-((5-amino-6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxypyridin-3-yl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (85 mg, yield: 90%) as a brown solid.

    [0813] MS m/z (ESI): 498.1 [M+H].sup.+.

    Step 8: Preparation of N-(5-((5-cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-6-methoxypyridin-3-yl)acrylamide

    [0814] ##STR00262##

    [0815] 2-((5-Amino-6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxypyridin-3-yl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (85 mg, 0.17 mmol) was dissolved in tetrahydrofuran (5 mL), and the solution was cooled to 0° C. Triethylamine (26 mg, 0.26 mmol) and 3-chloropropionyl chloride (33 mg, 0.26 mmol) were added successively. The reaction solution was stirred at 0° C. for 1 hour. 3M sodium hydroxide solution (2 mL) was added, and the reaction solution was stirred at 40° C. for 1 hour. Water (20 mL) was added, and the solution was extracted with dichloromethane (20 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by prep-TLC (dichloromethane/MeOH: 20/1) to obtain N-(5-((5-cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-6-methoxypyridin-3-yl)acrylamide (0.03 g, yield: 32%) as a yellow solid.

    [0816] MS m/z (ESI): 552.1 [M+H].sup.+.

    [0817] Method 2:

    ##STR00263##

    Step 1: Preparation of 6-chloro-2-methoxypyridin-3-amine

    [0818] ##STR00264##

    [0819] 6-Chloro-2-methoxy-nitropyridine (5 g, 26.6 mmol) was dissolved in ethanol (100 mL) and water (30 mL). Ammonium chloride (7.0 g, 133 mmol) was added, and iron powder (7.5 g, 133 mmol) was added in batches. The reaction solution was stirred at 85° C. for 2 hours. The reaction solution was cooled to room temperature, and filtered through celite. Ethyl acetate (150 mL) and saturated brine (120 mL) were added to the filtrate, and the organic layer was separated. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain 6-chloro-2-methoxypyridin-3-amine (4 g, yield: 95%) as a brown solid.

    [0820] MS m/z (ESI): 159.1 [M+H].sup.+.

    Step 2: Preparation of N-(6-chloro-2-methoxypyridin-3-yl)acetamide

    [0821] ##STR00265##

    [0822] 6-Chloro-2-methoxypyridin-3-amine (4.0 g, 25.0 mmol) was dissolved in dichloromethane (100 mL), followed by the addition of diisopropylethylamine (4.8 g, 37.5 mmol). The reaction solution was cooled to 0° C., to which acetyl chloride (2.4 g, 30.0 mmol) was added and stirred for 2 hours. The reaction solution was washed successively with 80 mL of water, 80 mL of 1N hydrochloric acid and 80 mL of saturated brine, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain N-(6-chloro-2-methoxypyridin-3-yl)acetamide (4.0 g, yield: 79%) as a brown solid.

    [0823] MS m/z (ESI): 201.1 [M+H].sup.+.

    Step 3: Preparation of N-(6-chloro-2-methoxy-5-nitropyridin-3-yl)acetamide

    [0824] ##STR00266##

    [0825] 6-Chloro-2-methoxypyridin-3-amine (2.0 g, 10.0 mmol) was dissolved in trifluoroacetic anhydride (20 mL), and cooled to −10° C. Fuming nitric acid (0.5 mL, 10 mmol) was added dropwise, and the reaction solution was stirred for 2 hours. Crushed ice was added to the reaction solution, and the solution was extracted with dichloromethane (50 mL). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain N-(6-chloro-2-methoxy-5-nitropyridin-3-yl)acetamide (1.6 g, yield: 65%) as a brown solid.

    [0826] MS m/z (ESI): 244.1 [M−H].sup.+.

    Step 4: Preparation of N-(6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropyridin-3-yl)acetamide

    [0827] ##STR00267##

    [0828] N-(6-chloro-2-methoxy-5-nitropyridin-3-yl)acetamide (1.6 g, 6.5 mmol) was dissolved in acetonitrile (30 mL), followed by the addition of N1,N1,N2-trimethylethane-1,2-diamine (1 g, 9.8 mmol). The reaction solution was stirred at 80° C. for 3 hours. The solvent was removed by rotary evaporation, and the resulting crude product was purified by column chromatography (dichloromethane/methanol: 100/1˜10/1) to obtain N-(6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropyridin-3-yl)acetamide (0.9 g, yield: 45%) as a brown solid.

    [0829] MS m/z (ESI): 312.1 [M+H].sup.+.

    Step 5: Preparation of N2-(2-(dimethylamino)ethyl)-6-methoxy-N2-methyl-3-nitropyridine-2,5-diamine

    [0830] ##STR00268##

    [0831] N-(6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropyridin-3-yl)acetamide (0.9 g, 2.9 mmol) was dissolved in methanol (30 mL) and concentrated hydrochloric acid (5 mL). The reaction solution was stirred at 60° C. for 3 hours. The solvent was removed by rotary evaporation, and dichloromethane (50 mL) and saturated sodium bicarbonate solution (50 mL) were added to the resulting residues and stirred until no bubbles generation. The organic layer was separated, dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by column chromatography (dichloromethane/methanol: 100/1˜10/1) to obtain N2-(2-(dimethylamino)ethyl)-6-methoxy-N2-methyl-3-nitropyridine-2,5-diamine (0.15 g, yield: 19%) as a brown solid.

    [0832] MS m/z (ESI): 270.1 [M+H].sup.+.

    Step 6: Preparation of 4-(1-cyclopropyl-1H-indol-3-yl)-2-((6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitropyridin-3-yl)amino)pyrimidine-5-carbonitrile

    [0833] ##STR00269##

    [0834] N2-(2-(Dimethylamino)ethyl)-6-methoxy-N2-methyl-3-nitropyridine-2,5-diamine (0.11 g, 0.41 mmol), 2-chloro-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (0.12 g, 0.41 mmol), tris(dibenzylideneacetone)dipalladium (0.18 g, 0.2 mmol), x-phos (0.2 g, 0.41 mmol) and sodium tert-butoxide (0.12 g, 1.2 mmol) were dissolved in dioxane (5 mL). The reaction solution was purged with nitrogen, and stirred under microwave at 140° C. for 1 hour. The reaction solution was cooled to room temperature and filtered. The filtrate was concentrated to dryness by rotary evaporation, and the resulting crude product was purified by prep-TLC (dichloromethane/methanol: 20/1) to obtain 4-(1-cyclopropyl-1H-indol-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (0.1 g, yield: 47%) as a yellow solid.

    [0835] MS m/z (ESI): 528.1 [M+H].sup.+.

    Step 7: Preparation of 2-((5-amino-6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxypyridin-3-yl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile

    [0836] ##STR00270##

    [0837] 4-(1-Cyclopropyl-1H-indol-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-m ethoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (80 mg, 0.15 mmol) was dissolved in methanol (10 mL), followed by the addition of Raney nickel (80 mg) and 85% hydrazine hydrate (90 mg, 1.5 mmol) at 0° C. The reaction solution was stirred at room temperature for 2 hours. The reaction solution was filtered, and the filtrate was concentrated to dryness by rotary evaporation. Water (15 mL) was added to the resulting residues, and the solution was extracted with dichloromethane (15 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation to obtain 2-((5-amino-6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxypyridin-3-yl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (80 mg, yield: 100% crude) as a brown solid.

    [0838] MS m/z (ESI): 498.1 [M+H].sup.+.

    Step 8: Preparation of N-(5-((5-cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-6-methoxypyridin-3-yl)acrylamide

    [0839] ##STR00271##

    [0840] 2-((5-Amino-6-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxypyridin-3-yl)amino)-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidine-5-carbonitrile (80 mg, 0.16 mmol) was dissolved in dichloromethane (10 mL), and the solution was cooled to 0° C. Triethylamine (24 mg, 0.24 mmol) and 3-chloropropionyl chloride (25 mg, 0.19 mmol) were added successively. The reaction solution was stirred at 0° C. for 1 hour. Water (10 mL) was added, and the solution was extracted with dichloromethane (10 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. Tetrahydrofuran (5 mL) and a solution of sodium hydroxide (64 mg, 1.6 mmol) in water (0.5 mL) were added, and the reaction solution was stirred at 40° C. overnight. Water (10 mL) was added, and the solution was extracted with ethyl acetate (10 mL×2). The organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to dryness by rotary evaporation. The resulting crude product was purified by prep-HPLC to obtain N-(5-((5-cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-6-methoxypyridin-3-yl)acrylamide (0.9 mg, yield: 0.7%) as a yellow solid. MS m/z (ESI): 552.1 [M+H].sup.+.

    Example 117

    N-(5-((5-Cyano-4-(1-cyclopropyl-4-methoxy-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0841] ##STR00272##

    [0842] The compound of Example 117 was prepared by referring to the method of Example 2.

    [0843] MS m/z (ESI): 581.1[M+H].sup.+.

    [0844] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.14 (s, 1H), 9.10-8.91 (br, 2H), 8.72 (s, 1H), 7.83 (s, 1H), 7.29-7.21 (m, 2H), 7.02 (s, 1H), 6.72 (d, J=7.6 Hz, 1H), 6.44-6.37 (m, 1H), 6.32-6.27 (m, 1H), 5.81-5.77 (m, 1H), 3.83 (s, 3H), 3.80 (s, 3H), 3.59-3.50 (m, 1H), 2.85-2.75 (m, 2H), 2.70 (s, 3H), 2.34-2.25 (m, 2H), 2.22 (s, 6H), 1.12-1.06 (m, 4H).

    Example 118

    N-(5-((5-Cyano-4-(1-cyclopropyl-5-methoxy-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0845] ##STR00273##

    [0846] The compound of Example 118 was prepared by referring to the method of Example 2.

    [0847] MS m/z (ESI): 581.4[M+H].sup.+.

    [0848] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.08-9.55 (m, 1H), 9.42 (s, 1H), 8.68 (s, 1H), 8.44 (s, 1H), 8.03 (s, 1H), 7.76 (s, 1H), 7.51 (d, J=8.6 Hz, 1H), 6.97 (d, J=8.6 Hz, 1H), 6.77 (s, 1H), 6.39 (d, J=15.7 Hz, 1H), 5.69 (d, J=10.5 Hz, 1H), 3.89 (s, 6H), 3.51-3.38 (m, 1H), 3.03 (s, 2H), 2.73 (s, 3H), 2.65-2.18 (m, 6H), 1.89-1.54 (m, 3H), 1.23-1.04 (m, 4H).

    Example 119

    N-(5-((5-Cyano-4-(1-cyclopropyl-7-methoxy-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0849] ##STR00274##

    [0850] The compound of Example 119 was prepared by referring to the method of Example 2.

    [0851] MS m/z (ESI): 581.3 [M+H].sup.+.

    [0852] .sup.1H NMR (400 MHz, Chloroform-d) δ 10.32-9.79 (m, 1H), 9.56-9.18 (m, 1H), 8.80-8.55 (m, 1H), 8.47-8.27 (m, 1H), 8.24-7.97 (m, 1H), 7.88-7.61 (m, 1H), 7.22-7.04 (m, 1H), 6.89-6.68 (m, 2H), 6.56-6.21 (m, 2H), 5.83-5.55 (m, 1H), 4.20-3.77 (m, 7H), 3.16-2.81 (m, 2H), 2.73 (s, 3H), 2.59-2.15 (m, 8H), 1.19-1.02 (m, 4H).

    Example 120

    N-(5-((5-Cyano-4-(1-cyclopropyl-4-fluoro-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0853] ##STR00275##

    [0854] The compound of Example 120 was prepared by referring to the method of Example 2.

    [0855] MS m/z (ESI): 569.2 [M+H].sup.+.

    [0856] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.22 (s, 1H), 8.90 (s, 1H), 8.75 (s, 1H), 8.69 (s, 1H), 8.27 (s, 1H), 7.48 (d, J=7.6 Hz, 1H), 7.28-7.23 (m, 1H), 7.09 (s, 1H), 6.92-6.87 (m, 1H), 6.46-6.39 (m, 1H), 6.30-6.26 (m, 1H), 5.78 (d, J=9.6 Hz, 1H), 3.84 (s, 3H), 3.61-3.52 (m, 1H), 2.96-2.92 (m, 2H), 2.75 (s, 3H), 2.37-2.29 (m, 2H), 2.22 (s, 6H), 1.16-1.04 (m, 4H).

    Example 121

    N-(5-((5-Cyano-4-(1-cyclopropyl-5-fluoro-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0857] ##STR00276##

    [0858] The compound of Example 121 was prepared by referring to the method of Example 2.

    [0859] MS m/z (ESI): 569.1[M+H].sup.+.

    Example 122

    N-(5-((5-Cyano-4-(1-cyclopropyl-7-fluoro-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0860] ##STR00277##

    [0861] The compound of Example 122 was prepared by referring to the method of Example 2.

    [0862] MS m/z (ESI): 569.4[M+H].sup.+.

    Example 123

    N-(5-((5-Cyano-4-(4-cyano-1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0863] ##STR00278##

    [0864] The compound of Example 123 was prepared by referring to the method of Example 2.

    [0865] MS m/z (ESI): 576.3[M+H].sup.+.

    Example 124

    N-(5-((5-Cyano-4-(5-cyano-1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0866] ##STR00279##

    [0867] The compound of Example 124 was prepared by referring to the method of Example 2.

    [0868] MS m/z (ESI): 576.2[M+H].sup.+.

    Example 125

    N-(5-((5-Cyano-4-(7-cyano-1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0869] ##STR00280##

    [0870] The compound of Example 125 was prepared by referring to the method of Example 2.

    [0871] MS m/z (ESI): 576.2[M+H].sup.+.

    Example 126

    N-(5-((5-Cyano-4-(1-cyclopropyl-5,6-dimethoxy-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0872] ##STR00281##

    [0873] The compound of Example 126 was prepared by referring to the method of Example 2.

    [0874] MS m/z (ESI): 611.1[M+H].sup.+.

    Example 127

    N-(5-((5-Cyano-4-(1-cyclopropyl-5-fluoro-6-methoxy-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0875] ##STR00282##

    [0876] The compound of Example 127 was prepared by referring to the method of Example 2.

    [0877] MS m/z (ESI): 599.2[M+H].sup.+.

    [0878] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.06 (s, 1H), 9.54 (s, 1H), 8.66 (s, 1H), 8.38 (s, 1H), 8.32 (s, 1H), 7.75-7.55 (m, 1H), 7.27-7.25 (m, 1H), 7.05 (s, 1H), 6.41-6.34 (m, 1H), 6.17 (d, J=16.9 Hz, 1H), 5.72 (d, J=10.3 Hz, 1H), 3.91 (s, 3H), 3.72 (s, 3H), 3.63-3.54 (m, 1H), 2.90-2.88 (m, 2H), 2.75 (s, 3H), 2.63-2.56 (m, 1H), 2.37-2.35 (m, 2H), 2.21 (s, 5H), 1.18-1.16 (m, 2H), 1.04-0.93 (m, 2H).

    Example 128

    N-(5-((5-Cyano-4-(6-cyclopropyl-2,3-dihydro-6H-[1,4]dioxino[2,3-f]indol-8-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0879] ##STR00283##

    [0880] The compound of Example 128 was prepared by referring to the method of Example 2.

    [0881] MS m/z (ESI): 609.3[M+H].sup.+.

    Example 129

    N-(5-((5-Cyano-4-(5-cyclopropyl-5H-[1,3]dioxolo[4,5-f]indol-7-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0882] ##STR00284##

    [0883] The compound of Example 129 was prepared by referring to the method of Example 2.

    [0884] MS m/z (ESI): 595.1[M+H].sup.+.

    Example 130

    N-(2-((2-(Dimethylamino)ethyl)(methyl)amino)-4-methoxy-5-((5-(3-methyl-1,2,4-oxadiazol-5-yl)-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0885] ##STR00285##

    [0886] The compound of Example 130 was prepared by referring to the method of Example 2.

    [0887] MS m/z (ESI): 582.4[M+H].sup.+.

    Example 131

    N-(2-((2-(Dimethylamino)ethyl)(methyl)amino)-4-methoxy-5-((5-(5-methyl-1,3,4-oxadiazol-2-yl)-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)acrylamide

    [0888] ##STR00286##

    [0889] The compound of Example 131 was prepared by referring to the method of Example 2.

    [0890] MS m/z (ESI): 582.2[M+H].sup.+.

    Example 132

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indazol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0891] ##STR00287##

    [0892] The compound of Example 132 was prepared by referring to the method of Example 2.

    [0893] MS m/z (ESI): 552.1[M+H].sup.+.

    Example 133

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-6-(2,2,2-trifluoroethoxy)pyridin-3-yl)acrylamide

    [0894] ##STR00288##

    [0895] The compound of Example 133 was prepared by referring to the method of Example 116.

    [0896] MS m/z (ESI): 620.3[M+H].sup.+.

    Example 134

    N-(5-((5-Cyano-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-6-(2,2,2-trifluoroethoxy)pyridin-3-yl)acrylamide

    [0897] ##STR00289##

    [0898] The compound of Example 134 was prepared by referring to the method of Example 116.

    [0899] MS m/z (ESI): 594.0[M+H].sup.+.

    Example 135

    N-(5-((5-Cyano-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-6-methoxypyridin-3-yl)acrylamide

    [0900] ##STR00290##

    [0901] The compound of Example 135 was prepared by referring to the method of Example 116.

    [0902] MS m/z (ESI): 526.3[M+H].sup.+.

    Example 136

    N-(5-((5-((Dimethyl(oxo)-λ.SUP.6.-sulfaneylidene)amino)-4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0903] ##STR00291##

    [0904] The compound of Example 136 was prepared by referring to the method of Example 36.

    [0905] MS m/z (ESI): 591.2 [M+H].sup.+.

    Example 137

    N-(5-((5-Cyano-4-(6-methoxy-1-(oxetan-3-yl)-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0906] ##STR00292##

    [0907] The compound of Example 137 was prepared by referring to the method of Example 111.

    [0908] MS m/z (ESI): 597.2 [M+H].sup.+.

    [0909] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.29-10.22 (br, 1H), 9.42 (s, 1H), 8.67 (s, 1H), 8.59 (m, 1H), 8.34 (s, 1H), 7.77 (s, 1H), 7.08 (s, 1H), 6.97-6.88 (br, 1H), 6.79 (s, 1H), 6.39 (d, J=14.4 Hz, 1H), 5.72-5.69 (m, 1H), 5.61-5.58 (m, 1H), 5.20 (d, J=6.4 Hz, 4H), 3.90 (s, 6H), 3.06-2.84 (br, 3H), 2.74 (s, 3H), 2.50-2.16 (br, 6H), 1.69-1.50 (br, 2H).

    Example 138

    N-(5-((5-Cyano-4-(3-methyl-1H-indazol-1-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0910] ##STR00293##

    [0911] The compound of Example 138 was prepared by referring to the following synthetic route.

    ##STR00294## ##STR00295##

    [0912] MS m/z (ESI): 526.3[M+H].sup.+.

    [0913] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.44-9.56 (m, 1H), 9.13 (s, 1H), 8.67 (s, 1H), 8.57-8.27 (m, 1H), 7.80-7.57 (m, 1H), 7.57-7.43 (m, 1H), 7.44-7.24 (m, 3H), 6.96-6.67 (m, 1H), 6.57-6.16 (m, 1H), 5.81-5.50 (m, 1H), 3.86 (s, 3H), 3.45-2.08 (m, 16H).

    Example 139

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0914] ##STR00296##

    Step 1: 1-Cyclopropyl-1H-pyrrolo[2,3-b]pyridine

    [0915] ##STR00297##

    [0916] Copper acetate (6.2 g, 34 mmol) was added to a mixture of 7-azaindole (4 g, 34 mmol), cyclopropylboronic acid (5.8 g, 68 mmol), 2,2′-bipyridine (5.3 g, 34 mmol) and sodium carbonate (7.2 g, 34 mmol) in 1,2-dichloroethane (100 ml). The reaction solution was heated to 85° C. under a nitrogen atmosphere, and reacted for 12 hours. The reaction solution was cooled, followed by the addition of water (150 ml) and filtration. The solid phase was rinsed with dichloromethane (60 ml), and the liquid phase was separated. The aqueous phase was extracted with dichloromethane (30 ml*2). The organic phases were combined, dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:dichloromethane=10:1) to obtain 1-cyclopropyl-1H-pyrrolo[2,3-b]pyridine (2.7 g, yield: 50.4%).

    [0917] MS m/z (ESI): 158.0[M+H].sup.+.

    Step 2: 2-Chloro-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidine-5-carbonitrile

    [0918] ##STR00298##

    [0919] Aluminum trichloride (1.07 g, 8.1 mmol) was added to a mixture of 2,4-dichloropyrimidine-5-carbonitrile (700 mg, 4.0 mmol) and 1-cyclopropyl-1H-pyrrolo[2,3-b]pyridine (637 mg, 4.0 mmol) in batches at 0° C. under a nitrogen atmosphere. The reaction was directly heated to 100° C. and stirred for 0.5 hour. The reaction solution was cooled to 0° C., to which methanol (10 ml) and water (30 ml) were added slowly. The solution was stirred at room temperature for 30 minutes, and extracted with dichloromethane (30 ml*2). The organic phases were combined, dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The crude product was purified by column chromatography (petroleum ether:dichloromethane=1:1) to obtain 2-chloro-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidine-5-carbonitrile (735 mg, yield: 62%).

    [0920] MS m/z (ESI): 296.0[M+H].sup.+.

    Step 3: 4-(1-Cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)-2-((4-fluoro-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile

    [0921] ##STR00299##

    [0922] P-toluenesulfonic acid monohydrate (643 mg, 3.38 mmol) was added to a solution of 4-fluoro-2-methoxy-5-nitroaniline (189 mg, 1.01 mmol) and 2-chloro-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidine-5-carbonitrile (250 mg, 0.85 mmol) in 2-butanol (15 ml). The reaction solution was heated to 90° C. for 12 hours. The reaction solution was cooled, and concentrated to dryness. Saturated aqueous sodium bicarbonate solution was added to adjust the pH to 9, and the solution was extracted with dichloromethane (35 ml*2). The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product. The crude product was purified by column chromatography (dichloromethane:ethyl acetate=5:1) to obtain the product 4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)-2-((4-fluoro-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (230 mg, yield: 61%).

    [0923] MS m/z (ESI): 446.1[M+H].sup.+.

    Step 4: 4-(1-Cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl) amino)-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile

    [0924] ##STR00300##

    [0925] Potassium carbonate (214 mg, 1.55 mmol) was added to a solution of N,N,N′-trimethylethylenediamine (58 mg, 0.57 mmol) and 4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)-2-((4-fluoro-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (230 mg, 0.52 mmol) in acetonitrile (10 mL). The reaction solution was heated to 80° C. and reacted for 2 hours. The reaction solution was cooled, and concentrated to dryness. The resulting residues were dissolved in dichloromethane (30 ml), and washed with brine (20 ml*3). The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product 4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (270 mg). The crude product was used directly in the next step.

    [0926] MS m/z (ESI): 528.2[M+H].sup.+.

    Step 5: 2-((5-Amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidine-5-carbonitrile

    [0927] ##STR00301##

    [0928] Iron powder (288 mg, 5.2 mmol) was added to a mixture of 4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)-2-((4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxy-5-nitrophenyl)amino)pyrimidine-5-carbonitrile (270 mg, 0.51 mol), ethanol (10 ml) and saturated aqueous ammonium chloride solution (4 ml). The reaction solution was heated to 80° C. and reacted for 2 hours. The reaction solution was filtered when it was still hot, the solid was rinsed with dichloromethane, and the liquid phase was concentrated to dryness to obtain the crude product. Dichloromethane (30 ml) was added to the crude product, and the solution was washed with water (15 ml*2). The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness to obtain the crude product 2-((5-amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidine-5-carbonitrile (250 mg), which was used directly in the next step.

    [0929] MS m/z (ESI): 498.3[M+H].sup.+.

    Step 6: 3-Chloro-N-(5-((5-cyano-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)propanamide

    [0930] ##STR00302##

    [0931] 3-Chloropropionyl chloride (77 mg, 0.60 mmol) was added to a solution of 2-((5-amino-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidine-5-carbonitrile (250 mg, 0.50 mmol) and triethylamine (0.21 ml, 1.51 mmol) in dichloromethane (10 ml) at 0° C. The reaction solution was reacted at 0° C. for 30 minutes. The reaction solution was concentrated to dryness to obtain the crude product 3-chloro-N-(5-((5-cyano-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)propanamide (250 mg), which was used directly in the next step.

    [0932] MS m/z (ESI): 588.3[M+H].sup.+.

    Step 7: N-(5-((5-Cyano-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide

    [0933] ##STR00303##

    [0934] Aqueous sodium hydroxide solution (containing sodium hydroxide (200 mg, 5 mmol) and water (1 ml)) was added to a solution of the crude 3-chloro-N-(5-((5-cyano-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)propanamide (290 mg, 0.50 mmol) in acetonitrile (10 ml). The reaction solution was stirred at room temperature for 16 hours. The reaction solution was concentrated to dryness to obtain the crude product. The crude product was dissolved in N,N-dimethylformamide (2 ml), and purified by preparative HPLC to obtain the product N-(5-((5-cyano-4-(1-cyclopropyl-1H-pyrrolo[2,3-b]pyridin-3-yl)pyrimidin-2-yl)amino)-2-((2-(dimethylamino)ethyl)(methyl)amino)-4-methoxyphenyl)acrylamide (55 mg, yield: 20%).

    [0935] MS m/z (ESI): 552.3[M+H].sup.+.

    [0936] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.32-9.88 (m, 1H), 9.59-9.18 (m, 1H), 8.92-8.52 (m, 3H), 8.52-8.26 (m, 1H), 7.96-7.61 (m, 1H), 7.24-6.99 (m, 1H), 6.82 (s, 1H), 6.55-6.15 (m, 2H), 5.85-5.52 (m, 1H), 3.89 (s, 3H), 3.72-3.58 (m, 1H), 3.01-2.83 (m, 2H), 2.74 (s, 3H), 2.51-2.19 (m, 8H), 1.30-1.04 (m, 4H).

    Example 140

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-(4-(3-ethoxyazetidin-1-yl)piperidin-1-yl)-4-methoxyphenyl)acrylamide

    [0937] ##STR00304##

    [0938] The compound of Example 140 was prepared by referring to the method of Example 67.

    [0939] MS m/z (ESI): 634.4[M+H].sup.+.

    [0940] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 12.90 (s, 1H), 9.35 (s, 1H), 8.67 (s, 1H), 8.48 (s, 1H), 8.38-8.27 (m, 1H), 7.79-7.71 (m, 1H), 7.62 (d, J=8.1 Hz, 1H), 7.36-7.28 (m, 2H), 6.88-6.79 (m, 1H), 6.41-6.27 (m, 2H), 5.75 (d, J=9.6 Hz, 1H), 4.63-4.47 (m, 2H), 3.91 (s, 3H), 3.78-3.62 (m, 2H), 3.55-3.43 (m, 3H), 3.30-3.03 (m, 4H), 2.77-2.68 (m, 2H), 2.24-1.97 (m, 4H), 1.23 (t, J=7.0 Hz, 3H), 1.20-1.06 (m, 4H).

    Example 141

    N-(5-((5-Cyano-4-(1-cyclopropyl-1H-indol-3-yl)pyrimidin-2-yl)amino)-2-((3aR,6aS)-5-cyclopropylhexahydropyrrolo[3,4-c]pyrrol-2(1H)-yl)-4-methoxyphenyl)acrylamide

    [0941] ##STR00305##

    [0942] The compound of Example 141 was prepared by referring to the method of Example 67.

    [0943] MS m/z (ESI): 601.2 [M+H].sup.+.

    [0944] .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.20 (400 MHz, CDCl9.35 (s, 1H), 8.67 (s, 1H), 8.8.41 (m, 2H), 7.84-8.27 (m, 1H), 7.79-7.71 (m, 1H), 7.62 (d, J=8.1 Hz, 1H), 7.36-7.28 (m, 2H), 6.88-6.79 (m, 1H), 6.41-6.27 (m, 2H), 5.75 (d, J=9.6 Hz, 1H), 3.85 (m, 3H), 3.55-3.41 (m, 2H), 3.38-3.18 (m, 2H), 3.15-2.92 (m, 5H), 2.83-2.60 (m, 2H), 2.07-1.96 (m, 1H), 1.32-1.23 (m, 4H), 1.21-1.10 (m, 4H).

    Example 142

    2-((5-Acrylamido-4-((2-(dimethylamino)ethyl)(methyl)amino)-2-methoxyphenyl)amino)-N-(4-fluorobenzyl)-4-(1-methyl-1H-indol-3-yl)pyrimidine-5-carboxamide

    [0945] ##STR00306##

    [0946] The compound of Example 142 was prepared by referring to the method of Example 5.

    [0947] MS m/z (ESI): 651.3 [M+H].sup.+.

    [0948] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 10.17-10.05 (m, 1H), 9.05-8.94 (m, 1H), 8.75-8.64 (m, 1H), 8.47-8.39 (m, 1H), 8.39-8.26 (m, 2H), 8.18-8.00 (m, 1H), 7.75 (s, 1H), 7.49-7.40 (m, 1H), 7.38-7.28 (m, 1H), 7.25-7.09 (m, 3H), 7.09-6.92 (m, 2H), 6.49-6.30 (m, 1H), 6.28-6.15 (m, 1H), 5.79-5.66 (m, 1H), 4.44-4.28 (m, 2H), 3.79 (s, 3H), 3.70 (s, 3H), 2.96-2.87 (m, 2H), 2.73 (s, 3H), 2.41-2.30 (m, 2H), 2.22 (s, 6H).

    Biological Assay and Evaluation

    [0949] The present invention is further illustrated below in combination with the following test examples, which are not intended to limit the scope of the present invention.

    [0950] 1. Enzymology Experiments

    Test Example 1. Determination of the Inhibitory Effect of the Compound of the Present Invention on the Kinase Activity of EGFR Exon 20 Insertion Mutation

    [0951] Experimental objective: The objective of this test example is to determine the inhibitory effect of the compound on the kinase activity of EGFR exon 20 insertion mutation.

    [0952] Experimental instruments: Centrifuge (Eppendorf 5810R), microplate reader (BioTek Synergy H1), pipette (Eppendorf & Rainin).

    [0953] Experimental process: The experiment applied the TR-FRET (time-resolved fluorescence resonance energy transfer) method to study the inhibitory effect of the compound on the kinase activity of EGFR exon 20 insertion mutation. The experiment was carried out in a 384-well plate. Experimental buffer (50 mM HEPES, 1 mM EGTA, 10 mM MgCl.sub.2, 2 mM DTT, 0.01% Tween-20) was formulated. The compound was diluted in gradient to different concentrations with the experimental buffer. 2.5 μL of the solution was added to each well of the 384-well plate. 2.5 μL of diluted EGFR kinase solution (0.001-0.5 nM) was added, and the plate was incubated at room temperature for 10 minutes. 5 μL of ULight-poly GT/ATP mixed solution was added, and the plate was incubated at room temperature for 30 to 60 minutes. 5 μL of EDTA stop solution and 5 μL of Eu-labeled antibody detection solution were added, and the plate was incubated at room temperature for 1 hour. The fluorescence signal value of each well at 665 nm was measured by the microplate reader.

    [0954] Experimental Data Processing Method:

    [0955] The inhibition rate (((positive control well value−sample well value)/(positive control well value−negative control well value))*100%) was calculated using the fluorescence signal value at 665 nm. The concentration and inhibition rate were fitted to a nonlinear regression curve by Graphpad Prism software to obtain the IC.sub.50 value. The specific data is shown in Table 1 below:

    TABLE-US-00001 TABLE 1 IC.sub.50 of the inhibitory effect of the compound on the kinase activity of EGFR exon 20 insertion mutation EGFR D770_N771insNPG Example No. IC.sub.50 (nM) AZD9291 2.89 24 0.62 26 0.36 35 0.43 37 0.56 38 0.45 40 0.55 43 0.29 45 1.00 52 0.79 53 0.50 57 0.82 63 0.62 64 0.39 65 0.29 67 0.14 68 0.17 74 0.64 77 0.91 78 0.50 81 0.47 85 0.27 109 0.78 110 0.53 111 0.18 112 0.17 113 0.70 114 0.61 118 0.55 119 0.49 127 0.18 137 0.16 139 0.68

    [0956] Experimental Conclusion:

    [0957] The above experiment demonstrates that the compounds of the examples of the present invention have a good inhibitory effect in the EGFR exon 20 insertion mutation kinase activity inhibition experiment.

    Test Example 2. Determination of the Inhibitory Effect of the Compound of the Present Invention on the Kinase Activity of Wild Type EGFR

    [0958] Experimental objective: The objective of this test example is to determine the inhibitory effect of the compound on the kinase activity of wild type EGFR.

    [0959] Experimental instruments: Centrifuge (Eppendorf 5810R), microplate reader (BioTek Synergy H1), pipette (Eppendorf & Rainin).

    [0960] Experimental process: The experiment applied the TR-FRET (time-resolved fluorescence resonance energy transfer) method to study the inhibitory effect of the compound on the kinase activity of wild type EGFR. The experiment was carried out in a 384-well plate. Experimental buffer (50 mM HEPES, 1 mM EGTA, 10 mM MgCl.sub.2, 2 mM DTT, 0.01% Tween-20) was formulated. The compound was diluted in gradient to different concentrations with the experimental buffer. 2 μL of the solution was added to each well of the 384-well plate. 4 μL of diluted EGFR kinase solution (0.001-0.5 nM) was added, and the plate was incubated at room temperature for 10 minutes. 4 μL of ULight-poly GT/ATP mixed solution was added, and the plate was incubated at room temperature for 30 to 60 minutes. 5 μL of EDTA stop solution and 5 μL of Eu-labeled antibody detection solution were added, and the plate was incubated at room temperature for 1 hour. The fluorescence signal value of each well at 665 nm was measured by the microplate reader.

    [0961] Experimental Data Processing Method:

    [0962] The inhibition rate (((positive control well value−sample well value)/(positive control well value−negative control well value))*100%) was calculated using the fluorescence signal value at 665 nm. The concentration and inhibition rate were fitted to a nonlinear regression curve by Graphpad Prism software to obtain the IC.sub.50 value. The specific data is shown in Table 2 below:

    TABLE-US-00002 TABLE 2 IC.sub.50 of the inhibitory effect of the compound on the kinase activity of wild type EGFR EGFR WT Example No. IC.sub.50 (nM) AZD9291 1.95 25 89.75 27 121.50 28 63.32 30 453.00 31 >1000 32 82.87 33 133.10 46 72.75 66 50.76 116 1324.00 120 >1000 136 250.00 138 >1000 142 133.10

    [0963] Experimental Conclusion:

    [0964] The above experiment demonstrates that the compounds of the examples of the present invention have a weak inhibitory effect in the EGFR wild type kinase activity inhibition experiment.

    Test Example 3. Determination of the Inhibitory Effect of the Compound of the Present Invention on the Proliferation of Ba/F3 EGFR Mutant Cell Line and A431 Cell Line

    [0965] Experimental objective: The objective of this test example is to determine the inhibitory effect of the compound on the proliferation of Ba/F3 EGFR mutant cell line and A431 cell line

    [0966] Experimental instruments: Microplate reader (BioTek Synergy H1), pipette (Eppendorf & Rainin).

    [0967] Experimental Process:

    [0968] Ba/F3 EGFR mutant cells were cultured, and harvested after reaching a suitable density. The cells were adjusted to an appropriate cell concentration with the complete medium. The cell suspension were spread on a 96-well plate (90 μL per well), and the plate was placed in a 37° C., 5% CO.sub.2 incubator overnight to let the cells adhere to the wall. Solutions of the compound at different concentrations were formulated with DMSO and culture medium. Solvent control was set. The solution of the compound was added to the 96-well plate (10 μL per well), and the plate was incubated in the 37° C., 5% CO.sub.2 incubator for 72 h to 144 h. CellTiter-Glo solution was added and mixed well by shaking, and the plate was incubated in the dark for 10 minutes. The values were measured by BioTek Synergy H1 microplate reader.

    [0969] Experimental Process:

    [0970] A431 cells were cultured, and harvested after reaching a suitable density. The cells were adjusted to an appropriate cell concentration with the complete medium. The cell suspension were spread on a 96-well plate (90 μL per well), and the plate was placed in a 37° C., 5% CO.sub.2 incubator overnight to let the cells adhere to the wall. Solutions of the compound at different concentrations were formulated with DMSO and culture medium. Solvent control was set. The solution of the compound was added to the 96-well plate (10 L per well), and the plate was incubated in the 37° C., 5% CO.sub.2 incubator for 72 h. CellTiter-Glo solution was added and mixed well by shaking, and the plate was incubated in the dark for 10 minutes. The values were measured by BioTek Synergy H1 microplate reader.

    [0971] Experimental Data Processing Method:

    [0972] The inhibition rate was calculated using the fluorescence signal value. The concentration and inhibition rate were fitted to a nonlinear regression curve by Graphpad Prism software to obtain the IC.sub.50 value. The specific data is shown in Table 3 below:

    TABLE-US-00003 TABLE 3 IC.sub.50 of the inhibitory effect of the compound on the proliferation of Ba/F3 EGFR mutant cell line and A431 cell line Ba/F3 EGFR-D770-N771ins_SVD A431 Example No. IC.sub.50 (nM) IC.sub.50 (nM) AZD9291 212.70 156.30 1 76.00 536.80 24 14.97 123.80 35 24.75 161.50 36 27.12 439.30 37 16.62 66.00 38 34.65 145.70 40 34.65 133.30 43 21.00 NA 45 53.44 209.80 47 33.13 51.73 52 34.88 306.50 53 21.65 182.20 56 45.32 144.40 57 28.08 113.30 63 14.97 NA 64 17.40 28.94 65 15.12 NA 67 10.41 94.54 68 15.40 NA 69 40.03 NA 70 47.36 NA 71 54.92 238.10 78 48.22 257.60 81 22.87 66.81 85 17.38 NA 109 49.14 167.90 111 21.17 34.08 112 26.69 NA 114 42.47 NA 118 45.09 200.60 119 45.44 NA 127 13.39 46.56 137 9.78 18.24

    [0973] Experimental Conclusion:

    [0974] The above experiment demonstrates that the compounds of the examples of the present invention have a good inhibitory effect in the Ba/F3 EGFR mutant cell proliferation inhibition experiment, but showed a poor inhibitory effect on A431 cell. By comparison, it can be seen that the compounds of the examples of the present invention have a high selectivity for the inhibition of Ba/F3 EGFR mutant cell proliferation.

    Test Example 4. In Vivo Pharmacodynamic Study of the Compound of the Present Invention in the Mouse Pro-B Cell Ba/F3 EGFR-D770-N771ins_SVD Xenograft Model

    [0975] 4.1 Experimental Objective:

    [0976] The in vivo efficacy of the compound in the mouse pro-B cell Ba/F3 EGFR-D770-N771ins_SVD xenograft model was evaluated.

    [0977] 4.2 Experimental instruments and reagents:

    [0978] 4.2.1 Instruments:

    [0979] 1. Biological safety cabinet (BSC-130011 A2, Medical equipment factory of Shanghai Boxun Industrial Co., Ltd.)

    [0980] 2. Clean bench (CJ-2F, Suzhou Fengshi Laboratory Animal Equipment Co., Ltd.)

    [0981] 3. CO.sub.2 incubator (Thermo-311, Thermo)

    [0982] 4. Centrifuge (Centrifuge 5720R, Eppendorf)

    [0983] 5. Automatic cell counter (Countess II, Life Technologies)

    [0984] 6. Vernier calipers (CD-6″ AX, Mitutoyo, Japan)

    [0985] 7. Cell culture flask (T75/T225, Corning)

    [0986] 8. Electronic balance (CPA2202S, Sartorius)

    [0987] 9. Electronic balance (BSA2202S-CW, Sartorius)

    [0988] 4.2.2 Reagents:

    [0989] 1. RPMI-1640 medium (22400-089, Gibco)

    [0990] 2. Fetal bovine serum (FBS) (10099-141C, Gibco)

    [0991] 3. Phosphate buffered saline (PBS) (10010-023, Gibco)

    [0992] 4. Tween 80 (30189828, Sinopharm Chemical Reagent Co., Ltd.)

    [0993] 5. Sodium carboxymethyl cellulose (30036365, Sinopharm Chemical Reagent Co., Ltd.)

    [0994] 4.3 Experimental Process and Data Processing:

    [0995] 4.3.1 Animals

    [0996] BALB/cA nude mice (6-8 weeks old, female) were purchased from Shanghai Sino-British SIPPR/BK Lab Animal Co., Ltd.

    [0997] 4.3.2 Cell Culture and Preparation of Cell Suspension

    [0998] a. A Ba/F3 EGFR-D770-N771ins_SVD cell line was taken from the cell bank, and the cells were resuscitated with the RPMI-1640 medium (RPMI-1640+10% FBS). The resuscitated cells were placed in the cell culture flasks (cell type, date, the name of the culturing person and the like were marked on the flask wall), and incubated in a CO.sub.2 incubator (the incubator temperature was 37° C., and the CO.sub.2 concentration was 5%).

    [0999] b. The cells were passaged every three days. After passage, the cells were further incubated in the CO.sub.2 incubator. The process was repeated until the number of cells met the requirements of the in vivo pharmacodynamic study.

    [1000] c. The cultured cells were collected and counted by the automatic cell counter. According to the counting results, the cells were resuspended in PBS to prepare a cell suspension (density: 2×10.sup.7 cells/mL), which was placed in an ice box for later use.

    [1001] 4.3.3 Cell Inoculation

    [1002] a. The nude mice were marked before inoculation with disposable universal ear tags for mouse and rat.

    [1003] b. At the time of inoculation, the cell suspension was mixed well, 0.1 to 1 mL of the cell suspension was taken with a 1 mL syringe, air bubbles were removed, and the syringe was placed on an ice bag for later use.

    [1004] c. The nude mice were bound with the operator's left hand. The right shoulder of the right back of the nude mouse (inoculation site) was disinfected with 75% alcohol. The nude mice were inoculated 30 seconds later.

    [1005] d. The test nude mice were inoculated in turn (0.1 mL of cell suspension per mouse).

    [1006] 4.3.4 Tumor Volume Measurement, Grouping and Administration in the Tumor-Bearing Mouse

    [1007] a. Tumor was measured on Day 8 to Day 14 after inoculation depending on the tumor growth, and tumor size was calculated.

    [1008] Tumor volume calculation: tumor volume (mm.sup.3)=length (mm)×width (mm)×width (mm)/2

    [1009] b. According to the body weight and tumor size of the tumor-bearing mouse, the mice were randomly grouped (5 mice per group).

    [1010] c. According to the grouping results, the test drug was administered (administration method: oral administration; administration frequency: 1 time/day; administration period: 14 days; vehicle: 0.5% CMC/1% Tween 80).

    [1011] d. The tumor was measured and the mouse was weighed twice a week after the test drug was administrated.

    [1012] e. The animals were euthanized at the end of the test.

    [1013] f. The data were processed with software such as Excel.

    [1014] Calculation of the Tumor Growth Inhibition Rate TGI (%) of the Compound:


    TGI (%)=[(1−average tumor volume at the end of the administration in a certain treatment group)/average tumor volume at the end of the administration in the solvent control group]×100%.

    [1015] TGI≥60% indicates that the compound is effective in this model;

    [1016] TGI<60% indicates that the compound is ineffective in this model.

    [1017] 4.4 The Test Results are Shown in Table 4 Below:

    TABLE-US-00004 TABLE 4 Pharmacodynamic parameters of the compound in xenograft mouse Tumor volume (mm3, Mean ± Administration SD) T/C (%) TGI (%) Group dose Day 14 Day 14 Day 14 ADZ9291 40 mg/kg  935 ± 176 48.40 51.60 (1932 ± 123) Example 37 40 mg/kg  499 ± 151 25.75 74.25 (1938 ± 197) Example 67 40 mg/kg 212 ± 44 10.94 89.06 (1938 ± 197) Example 81 40 mg/kg  334 ± 121 17.23 82.77 (1938 ± 197) Note: The data in parentheses refer to the tumor volume of the corresponding Vehicle QD × 2w group (i.e., the control group) at the corresponding time.

    [1018] 4.5 Experimental Results

    [1019] The above data demonstrates that: after 14 days of continuous oral administration, AZD9291 40 mpk is ineffective in the Ba/F3 EGFR-D770-N771ins_SVD pharmacodynamic model, while the compounds of Examples 37, 67 and 81 are effective at the same dose of 40 mpk.

    Test Example 5. Plasma Stability Test

    [1020] 5.1 Experimental Objective

    [1021] The objective of this experiment is to determine the stability of the compounds of the examples in the plasma of mouse, rat and human.

    [1022] 5.2 Experimental Steps

    [1023] 5.2.1 Solution Formulation

    [1024] 1. Plasma preparation: Animal or human whole blood was collected, placed into a test tube containing anticoagulant, and centrifuged at 3500 rpm for 10 min. The light yellow plasma in the upper layer was collected.

    [1025] 2. 1 mM test compound (m/M/V=C), the compound was weighed and formulated into stock solution with DMSO.

    [1026] 5.2.2 Experimental Process:

    [1027] 1. 398 μL of plasma and 2 μL of 1 mM compound (test compound) were added successively to a 96-well plate, and incubated at 37° C.

    [1028] 2. 50 μL of solution was taken out at 0, 15, 30, 60, 120 min, to which 400 μL of methanol stop solution containing internal standard was added.

    [1029] 3. The solution was centrifuged (3220 g, 30 min). 50 μL of supernatant was taken out, to which 50 μL of DDH.sub.2O was added to dilute. The resulting solution was injected into a LC-MS/MS.

    [1030] 5.3 Chromatography Conditions

    [1031] Instrument: Shimadzu LC-30AD (Nexera X2)

    [1032] Column: XSelect HSS T3 C.sub.18 (50*2.1 mm, particle size: 2.5 m);

    [1033] Mobile Phases:

    [1034] A: 0.1% formic acid solution,

    [1035] B: Acetonitrile containing 0.1% formic acid,

    [1036] 0˜0.3 min: 95% A.fwdarw.95% A;

    [1037] 0.3˜0.8 min: 95% A.fwdarw.5% A;

    [1038] Flow rate: 0.5 mL/min;

    [1039] Running time: 2.0 min;

    [1040] Injection volume: 3 μL.

    [1041] 5.4 Mass spectrometry conditions

    [1042] Instrument:

    [1043] API4000 Liquid Chromatography-Mass Spectrometer, AB Company, USA;

    [1044] The ion source was an electrospray ionization source (ESI);

    [1045] The drying gas (N2) temperature was 500° C.;

    [1046] The electrospray voltage was 5500V;

    [1047] The detection method was positive ion detection;

    [1048] The scanning method was the selective reaction monitoring (MRM) method.

    TABLE-US-00005 TABLE 5 Results of plasma stability of the compounds of the examples Residual rate (%) 0 15 30 60 120 t.sub.1/2 Species No. min min min min min (min) Human Example 67 100.00 93.86 85.71 67.74 37.38 82.71 Rat Example 67 100.00 90.61 81.14 59.66 33.25 74.04 Mouse Example 67 100.00 88.69 69.92 36.29 9.06 39.89

    [1049] 5.5 Experimental Conclusion:

    [1050] The above data demonstrate that the compounds of the examples of the present invention have high plasma stability and small differences between species.