INLAY FOR A FLASK AND METHOD FOR INSERTING AN INLAY INTO A FLASK

20250011702 ยท 2025-01-09

    Inventors

    Cpc classification

    International classification

    Abstract

    An inlay for a flask comprising a bottom portion, a side portion, an intermediate portion, and a baffle element. The bottom portion comprises a downward-facing or downward-and-outwardfacing first surface for abutting a bottom inner surface of the flask. The side portion comprises an outer surface for abutting a side inner surface of the flask, wherein the outer surface faces outwards with respect to a vertical reference line intersecting the inlay. The intermediate portion mechanically connects the bottom portion and the side portion. The baffle element extends away from the bottom portion, from the side portion, and/or from the intermediate portion for extending into an inner volume of the flask. An inner angle between the first surface and the outer surface is at least 50, wherein the inner angle refers to an angle on the side of the vertical reference line in a vertical cross section through the inlay. The inlay comprises a first flexible material adapted to allow for bending said side portion inwards. The inlay is adapted to allow for folding the inlay with respect to a horizontal crease line to insert the inlay into the flask. The inlay comprises, along a vertical plane comprising the horizontal crease line, a second flexible material to allow for said folding.

    Claims

    1. An inlay for a flask, the inlay comprising: a bottom portion comprising a downward-facing or downward-and-outward-facing first surface for abutting a bottom inner surface of the flask; a side portion comprising an outer surface for abutting a side inner surface of the flask, wherein the outer surface faces outwards with respect to a vertical reference line intersecting the inlay; an intermediate portion mechanically connecting the bottom portion and the side portion; and a baffle element extending away from the bottom portion, from the side portion, and/or from the intermediate portion for extending into an inner volume of the flask; wherein an inner angle between the first surface and the outer surface is at least 50, wherein the inner angle refers to an angle on the side of the vertical reference line in a vertical cross section through the inlay; and wherein the inlay comprises a first flexible material adapted to allow for bending said side portion inwards; wherein the inlay is adapted to allow for folding the inlay with respect to a horizontal crease line to insert the inlay into the flask; and wherein the inlay comprises, along a vertical plane comprising the horizontal crease line, a second flexible material to allow for said folding.

    2. The inlay according to claim 1, wherein a ratio of a height of the inlay along the vertical direction and a maximum width of the inlay in a horizontal direction is at most 0.5.

    3. The inlay according to claim 1, wherein the inlay is an integral part.

    4. The inlay according to claim 1, wherein the intermediate portion or an outward-facing surface of the intermediate portion extends from the outer surface to the first surface.

    5. The inlay according to claim 1, wherein the first flexible material is a first elastomer, wherein: the first elastomer is adapted to promote a force-fit connection of the first surface to the bottom inner surface of the flask and/or to promote a force-fit connection of the outer surface to the side inner surface of the flask.

    6. The inlay according to claim 5, wherein the first elastomer is adapted to, upon the bending the side portion inwards, provide an elastic restoration force against the bending, said restoration force promoting said force-fit connection of the side portion to the side inner surface of the flask and/or of the first surface to the bottom inner surface of the flask (30).

    7. The inlay according to claim 1, wherein the downward-facing or downward-and-outward-facing first surface is a downward-facing bottom surface of the bottom portion.

    8. The inlay according to claim 1, wherein, in a horizontal plane intersecting the bottom portion, a shape of the bottom portion is a ring segment or a ring.

    9. The inlay according to claim 1, wherein the bottom portion and the first surface comprise an opening.

    10. The inlay according to claim 5, wherein the bottom portion comprises or is composed of the first elastomer.

    11. The inlay according to claim 1, wherein the first surface extends in a horizontal plane.

    12. The inlay according to claim 1, wherein the bending the side portion inwards bends the side portion towards the vertical reference line and/or reduces the inner angle, wherein, the elastic restoration force acts to bend the side portion away from the vertical reference line and to increase the inner angle.

    13. The inlay according to claim 1, wherein the baffle element extends upwards from the bottom portion.

    14. The inlay according to claim 1, wherein the flask is an Erlenmeyer flask.

    15. An inlay for a flask, the inlay comprising: a bottom portion comprising a downward-facing for abutting a bottom inner surface of the flask; a side portion comprising an outer surface for abutting a side inner surface of the flask, wherein the outer surface faces outwards with respect to a vertical reference line intersecting the inlay; an intermediate portion mechanically connecting the bottom portion and the side portion; and a baffle element extending away from the bottom portion for extending into an inner volume of the flask; wherein an inner angle between the first surface and the outer surface is at least 50, wherein the inner angle refers to an angle on the side of the vertical reference line in a vertical cross section through the inlay; and wherein the inlay comprises a first flexible material adapted to allow for bending said side portion inwards; wherein the inlay is adapted to allow for folding the inlay with respect to a horizontal crease line to insert the inlay into the flask; and wherein the inlay comprises, along a vertical plane comprising the horizontal crease line, a second flexible material to allow for said folding.

    16. A method for inserting an inlay into a flask, the inlay comprising a second flexible material and a baffle element, the method comprising: folding the second flexible material with respect to a crease line; aligning the crease line parallel to an axis of the flask; inserting the folded inlay into the flask with the crease line aligned with the axis of the flask; and unfolding, unfolding the second flexible material, the inlay in the flask, such that a downward-facing or downward-and-outward-facing first surface and/or an outer surface of the inlay abuts an inner surface of the flask, and that the baffle element extends into an inner volume of the flask.

    17. The method of claim 16, wherein the second flexible material is a second elastomer, wherein the second elastomer promotes the unfolding the inlay in the flask.

    18. The method of claim 17, wherein the inlay is unfolded by the second elastomer such that the crease line is orientated orthogonal to the axis of the flask.

    19. The method of claim 16, wherein the inlay comprises a bottom portion comprising the first surface, a side portion comprising the outer surface, an intermediate portion mechanically connecting the bottom portion and the side portion, and a first flexible material, wherein the unfolding comprises: arranging the first surface to abut a bottom inner surface of the flask; and arranging the outer surface to abut a side inner surface of the flask.

    20. The method of claim 19, wherein the first flexible material is an elastomer, and wherein the unfolding comprises: providing, by the first elastomer, a force-fit connection of the first surface to the bottom inner surface of the flask and/or a force-fit connection of the outer surface to the side inner surface of the flask.

    Description

    BRIEF DESCRIPTION OF THE DRAWINGS

    [0076] The techniques of the present disclosure and the advantages associated therewith will be best apparent from a description of exemplary embodiments in accordance with the accompanying drawings, in which:

    [0077] FIG. 1a is a top view of an inlay according to a first embodiment;

    [0078] FIG. 1b is a sectional view of the inlay according to the first embodiment;

    [0079] FIG. 2a is a sectional view of an inlay according to another embodiment;

    [0080] FIG. 2b is a sectional view of an inlay according to another embodiment;

    [0081] FIG. 3a is sectional views of inlays according to other embodiments;

    [0082] FIG. 3b is sectional views of inlays according to other embodiments;

    [0083] FIG. 3c is a sectional view of an inlay according to another embodiment;

    [0084] FIG. 3d is a sectional view of an inlay according to another embodiment;

    [0085] FIG. 4a is a perspective view of an inlay according to another embodiment;

    [0086] FIG. 4b is another perspective view of the inlay according to the embodiment of FIG. 4a;

    [0087] FIG. 4c is a side view of the inlay according to the embodiment of FIG. 4a, FIG. 4b;

    [0088] FIG. 5a illustrates a method for inserting an inlay into a flask according to an embodiment;

    [0089] FIG. 5b further illustrates the method for inserting the inlay into the flask;

    [0090] FIG. 5c further illustrates the method for inserting the inlay into the flask;

    [0091] FIG. 5d further illustrates the method for inserting the inlay into the flask;

    [0092] FIG. 6 illustrates a method for inserting an inlay into a flask according to another embodiment;

    [0093] FIG. 7a is a perspective view of an inlay according to an embodiment with a baffle element extending upwards from a bottom portion;

    [0094] FIG. 7b is a perspective view of an inlay according to another embodiment with a baffle element extending upwards from a bottom portion;

    [0095] FIG. 7c is a perspective view of an inlay according to another embodiment with a baffle element extending upwards from a bottom portion;

    [0096] FIG. 7d is a perspective view of an inlay according to another embodiment with a baffle element extending upwards from a bottom portion;

    [0097] FIG. 7e is a perspective view of an inlay according to another embodiment with a baffle element extending upwards from a bottom portion;

    [0098] FIG. 8a is a perspective view of an inlay according to an embodiment with a baffle element extending inwards from a side portion;

    [0099] FIG. 8b is a perspective view of an inlay according to another embodiment with a baffle element extending inwards from a side portion;

    [0100] FIG. 8c is another perspective view of the embodiment of FIG. 8b;

    [0101] FIG. 8d is a perspective view of an inlay according to another embodiment with a baffle element extending inwards from a side portion;

    [0102] FIG. 8e is another perspective view of the embodiment of FIG. 8d;

    [0103] FIG. 8f is a cross-sectional view of the embodiment of FIG. 8d and FIG. 8e;

    [0104] FIG. 9a is a perspective view of a body with second openings;

    [0105] FIG. 9b is a perspective view of a body with second openings and recesses;

    [0106] FIG. 9c is a perspective view of a body with light-emitting diodes; and

    [0107] FIG. 10 illustrates oxygen transfer rates.

    DETAILED DESCRIPTION

    [0108] FIG. 1a, FIG. 1b show an inlay 10 according to a first embodiment. FIG. 1a is a top view, and FIG. 1b is a cross-sectional view along the vertical x, z plane 28 comprising the line c in FIG. 1a. In FIG. 1b, a bottom portion of a flask 30 (not shown in FIG. 1a) is indicated by dashed lines.

    [0109] The inlay 10 comprises a bottom portion 2 and a side portion 12. An intermediate portion 22, which, in the depicted embodiment, is the edge between the bottom portion 2 and the side portion 12, and connects the portions 2, 12.

    [0110] The bottom portion 2 has a first surface 4, adapted to abut a bottom inner surface 32 of a flask 30 (not shown in FIGS. 1a). In the depicted embodiment, the first surface 4 faces downwards, but in other embodiments, it is a downward-and-outward-facing surface 4 (see FIG. 3d).

    [0111] The side portion 12 has an outer surface 14, which is adapted to abut a side inner surface 34 of the flask 30.

    [0112] The inner angle 18 between the first surface 4 and the outer surface 14 is selected according to the shape of a flask 30, that the inlay 10 is to be inserted into, and amounts to at least 50, and preferably at least 70 to implement an inlay 10 for an Erlenmeyer flask.

    [0113] Baffle elements 20 (stirring elements 20) in the form of fins 20 extend upwards from the bottom portion 2. In alternative embodiments, see FIG. 8a, FIG. 8b, FIG. 8c, FIG. 8d, FIG. 8c, the baffle elements 20 extend inwards from the side portion 12.

    [0114] Where the vertical plane 28 intersects the inlay 10, the inlay comprises a (first) flexible material. Consequently, the inlay 10 can be folded along the line c. In this sense, line c serves as a crease line.

    [0115] The side portion 12 of the inlay 10 can be bent inwards towards the axis a. For this purpose, the bottom portion 2 in the vicinity of the intermediate portion 22, the intermediate portion 22, and/or the side portion 12 comprises a flexible material.

    [0116] In the embodiment depicted in FIG. 1a, FIG. 1b, the outer contour of the bottom portion 2 as well as the outer contour of the side portion 12 is a full circle, i.e., in the top view of FIG. 1a. In alternative embodiments (not shown), this full circle is interrupted. For example, the height of the side portion 12 and/or of the intermediate portion 22 and/or of the baffle element(s) 20 may be reduced above the crease line c or the respective element may be absent above the crease line c to facilitate easier folding with respect to the crease line c. In alternative embodiments (not shown), the outer contour of the bottom portion 2 as well as the outer contour of the side portion 12 is a square or a rectangle, i.e., in a top view.

    [0117] Thicknesses t1, t2 of the bottom and the side portions 2, 12, respectively, are preferably no more than 6 mm, 5 mm, or 4 mm, to ensure that the material of the respective portion is flexible.

    [0118] FIG. 2a, FIG. 2b illustrate the folding of the inlay 10, giving cross sectional views of folded inlays according to different embodiments.

    [0119] The inlay 10 of FIG. 2a comprises rigid baffle elements 20. The rigid baffle elements 20 may be advantageous in some embodiments to ensure a high oxygen transfer rate.

    [0120] The inlay 10 of FIG. 2b comprises flexible baffle elements 20, i.e., baffle elements 20 comprising a flexible material. Advantageously, in this embodiment, the inlay 10 can be folded to a smaller height (as compared to the inlay 10 of the embodiment depicted in FIG. 2a). This makes inserting the inlay 10 into a flask easier, faster, and more reliable.

    [0121] FIG. 3a, FIG. 3b, FIG. 3c, FIG. 3d illustrate various embodiments of the inlay 10 for different flasks 30.

    [0122] Before going into the details of the individual embodiments, common properties of all the depicted embodiments will be described. These do not only apply to the embodiments of FIG. 3a, FIG. 3b, FIG. 3c, FIG. 3d, but as well to the other embodiments of this description.

    [0123] In some embodiments, the inlay 10 is fixed in the flask 30, i.e., using a fixing element such as a glue or a resin. In corresponding embodiments, the first and/or second flexible material can be selected from a wide range of materials, including plastics, for as long as they are sufficiently thin to be flexible and allow for a bending with respect to the crease line c (see FIG. 2a, FIG. 2b) by more than 90. The first and/or second flexible material can as well be an elastomer, such as silicone or rubber.

    [0124] However, preferably, the inlay 10 is adapted to be held in place in the flask 10 using force-fit connections, i.e., a force-fit connection of the first surface 4 to the bottom inner surface 32 of the flask and/or a force-fit connection of the outer surface 14 to the side inner surface 34 of the flask 30. For this purpose, the first and/or second flexible material is/are an elastomer material.

    [0125] The second elastomer material, along the crease line c, provides a restoration force against the folding of the inlay 10 with respect to the crease line c. In other words, when the force which caused the folding is released, the inlay 10 is unfolded by the second elastomer, or by the restoration force it provides, respectively. This pushes the outer surface 14 against the side inner surface 34 of the flask 30, and the first surface 4 against the bottom inner surface 32 of the flask 30, promoting said force-fit connections.

    [0126] The first elastomer material, comprised in the side portion 12, the intermediate portion 22, and/or the bottom portion 2 in the vicinity of the intermediate portion 22, provides an elastic restoration force against the bending inwards of the side portion 12. Consequently, this restoration force pushes the outer surface 14 against the side inner surface 34 of the flask 30, and the first surface 4 against the bottom inner surface 32 of the flask, further promoting said force-fit connections.

    [0127] The advantage of the embodiments adapted to provide the force-fit connection(s) over the ones fixed in the flask 30 is rooted in the force-fit connection(s) being detachable. Consequently, the inlay 10 can be removed from the flask 30 without damaging or destroying it, and can be reused after cleaning or in another flask 30.

    [0128] The first and/or second elastomer material is/are typically rubber or silicone. Those also offer the advantage that the flask 30 can be autoclaved with the inlay 10 inside.

    [0129] Inlays 10 for being fixed to the flask 30 have inner angles 18 corresponding to the inner angle between the bottom inner wall 32 and the side inner wall 34 of the respective flask 30.

    [0130] Inlays 10 for force-fit connection(s) to the flask 30 have inner angles 18 slightly larger than the inner angle between the bottom inner wall 32 and the side inner wall 34 of the respective flask 30. A difference of at least 1 is preferred, for example a difference of 1 or 2 has proven sufficient.

    [0131] FIG. 3a illustrates inlays 10 for wide-neck Erlenmeyer flasks 30 according to ISO 24450. The inner angles between the bottom inner walls 32 and the side inner walls 34 of those Erlenmeyer flasks 30 are in the range from 71-79, with generally larger angles for smaller flasks. The inlay 10 for the wide-neck Erlenmeyer flasks 30 has an inner angle 18 of at least 70.

    [0132] FIG. 3b illustrates inlays 10 for narrow-neck Erlenmeyer flasks 30 according to ISO 1773. The inner angles between the bottom inner walls 32 and the side inner walls 34 of those Erlenmeyer flasks 30 are in the range from 75-79, with generally larger angles for smaller flasks. The inlay 10 for the narrow-neck Erlenmeyer flasks 30 has an inner angle 18 of at least 75.

    [0133] FIG. 3c illustrates an inlay 10 for a flask 30 with vertical inner side walls, i.e., with an inner angle 18 of 90 between the bottom inner wall 32 and the side inner walls 34 of the flask 30.

    [0134] FIG. 3d illustrates an inlay 10 for a flask 30 typically used with a centrifuge (not shown). This type of flask 30 has an inner angle 18 in the range from 95 to 120 between its bottom inner wall 32 and its side inner walls 34.

    [0135] Fernbach flasks (not shown) have inner angles between 50 and 70. An inlay (not shown) for a Fernbach flask has an inner angle 18 of at least 50.

    [0136] FIG. 4a, FIG. 4b, FIG. 4c illustrate another embodiment of the inlay 10. FIG. 4a, FIG. 4b give different perspective views of the inlay 10 according to the embodiment. FIG. 4c is a side view.

    [0137] The inlay 10 of FIG. 4a, FIG. 4b, FIG. 4c is formed with an opening 16.

    [0138] The opening 16 makes it easier to fold the inlay 10 and it also improves the fit of the inlay 10 to the flask 30, in particular of the first surface 4 to the bottom inner surface 32 of the flask 30. Moreover, the opening 16 reduces the amount of material needed to fabricate the inlay 10.

    [0139] The opening 16 may also be formed with any of the other embodiments.

    [0140] The opening 16 comprises the center of the first surface 4. In the depicted embodiment, it is centered, but it may also be arranged slightly off-center. In the depicted embodiment, the opening 16 has a circular cross-section, but alternative cross-sections are possible, for as long as the amount of material of the bottom surface 2 is reduced sufficiently to improve the folding and the fit to the flask 30. A diameter r1 (FIG. 4a) of the opening 16 in the range from 1-3 cm has proven useful for this purpose. In case of a non-circular cross section, a width thereof is selected in the same range.

    [0141] The intermediate portion 22 of the inlay 10 has an outward-facing surface 24, with a convex (i.e., outward-curved) shape, facing downward and outward. The curvature of the outward-facing surface 24 (FIGS. 4b, 4c) is matched to the curvature of a typical lab flask 30 at the transition from its bottom inner wall 32 to its side inner walls 34. More specifically, the curvature is in the range from 6 mm to 6 cm, matched to the respective curvature of Erlenmeyer flasks 30 according to ISO 1773 or ISO 24450.

    [0142] A corresponding outward-facing surface 24 may also be formed on any of the other embodiments.

    [0143] The diameter R (cf. FIG. 4c) of the inlay 10 is in the range of 4 to 30 cm, matched to the maximum inner diameter of Erlenmeyer flasks 30 according to ISO 1773 or ISO 24450. Typically, the diameter R is selected with a value of 1 to 5 mm larger than an inner diameter of the flask 30 at its widest position.

    [0144] The height H (cf. FIG. 4c) of the inlay 10 is in the range from 0.5 cm to 10 cm, depending on the size of the flask. Within this range, a smaller height H improves the folding, whereas a larger height H improves the adhesion to the side inner wall of the flask 30. The range from 0.5 cm to 6 cm ensures the balancing of both properties.

    [0145] The width w of the ring-shaped bottom portion of the inlay 10 is approximately 1 cm in case of an inlay for a 250 ml Erlenmeyer flask.

    [0146] FIG. 5a, FIG. 5b, FIG. 5c, FIG. 5d illustrate a method for inserting an inlay 10 into a flask 30.

    [0147] FIG. 5a shows the inlay 10 outside of the flask 30, as is the case at the beginning of the method. The flask 30 is an Erlenmeyer flask 30 with an axis A.

    [0148] FIG. 5b shows the folding of the inlay 10 with respect to the crease line c.

    [0149] FIG. 5b further shows that the inlay 10 with its crease line c is aligned with the axis A of the flask 30.

    [0150] FIG. 5c shows the inlay 10 and the flask 30, after the inlay 10 has been inserted with its crease line c aligned with the axis A of the flask 30 (as depicted in FIG. 5b). The inlay 10 is arranged fully below the neck portion of the flask 30.

    [0151] In FIG. 5c, the inlay 10 is being unfolded. In the depicted embodiment, the inlay 10 is an integral piece of elastomer, more specifically of silicone, formed by molding. As the forces applied to the inlay 10 during the insertion into the flask 30 (FIG. 5b) are released in FIG. 5c, the elastomer provides a restoring force driving the inlay 10 back into its original shape as depicted in FIG. 5a.

    [0152] In alternative embodiments (not shown), the inlay 10 is not elastic, i.e., it does not comprise an elastomer. In corresponding embodiments, an additional action is required to unfold the inlay 10 inside of the flask 30. In other words, a machine, a tool or a worker grasps into the flask 30 and unfolds the inlay 10.

    [0153] FIG. 5d shows the inlay 10 in its final state in the flask 30 after the unfolding.

    [0154] In some embodiments (not shown) the inlay 10 is fixed in the flask 30, for example with glue. In yet other embodiments (not shown) a portion of the inlay 10 is removed, by etching or ashing (i.e., heating the flask 30 to a temperature above a decomposition temperature of the material of the inlay 10).

    [0155] FIG. 6 illustrates a method 60 for inserting an inlay 10 into a flask 30 according to an alternative embodiment. The inlay 10 comprises a second flexible material and a baffle element 20.

    [0156] The method 60 begins with folding 62 the second flexible material with respect to a crease line c.

    [0157] The method 60 continues with aligning 64 the crease line c parallel to an axis A of the flask 30.

    [0158] The method 60 continues with inserting 66 the folded inlay 10 into the flask 30 with the crease line c aligned with the axis A of the flask 30.

    [0159] The method further comprises unfolding 68, unfolding the second flexible material, the inlay 10 in the flask 30, such that a downward-facing or downward-and-outward-facing first surface 4 and/or an outer surface 14 of the inlay 10 abuts an inner surface 32, 34 of the flask 30, and that the baffle element 20 extends into an inner volume 36 of the flask 30.

    [0160] FIG. 7a, FIG. 7b, FIG. 7c, FIG. 7d, FIG. 7e give perspective views of inlays 10 according to various embodiments with a baffle element 20 extending upwards from the bottom portion 2.

    [0161] In the embodiments of FIG. 7a, FIG. 7b, FIG. 7c, the baffle element 20 comprises a c-shaped, or half-moon-shaped element 70. The respective element has an extrusion shape, with a c-shaped, or half-moon-shaped horizontal basis in a horizontal plane. The extrusion refers to an extrusion along the vertical direction, resulting in the height of the respective baffle element 20.

    [0162] The inlay 10 of FIG. 7a comprises a single c-shaped, or half-moon-shaped element 70.

    [0163] The inlay 10 of FIG. 7b comprises two c-shaped, or half-moon-shaped elements 70, arranged opposite to each other, with an interruption along the azimuthal direction.

    [0164] The inlay 10 of FIG. 7c comprises a single, extended c-shaped element 70 covering approximately 270 (i.e., more than 180, and/or more than) 230 along the azimuthal direction. The c-shaped element 70 of FIG. 7c preferably comprises an interruption along the azimuthal direction.

    [0165] In the embodiments of FIG. 7d, FIG. 7e, the baffle element 20 comprises fins extending upwards from the bottom portion 2. The fins have extrusion shapes with bases in a respective horizontal plane.

    [0166] At least 4 or at least 8 or at least 10 fins are preferable. The embodiment of FIG. 7d comprises 12 fins. The embodiment of FIG. 7e comprises 40 fins.

    [0167] FIG. 8a, FIG. 8b, FIG. 8c, FIG. 8d, FIG. 8e, FIG. 8f illustrate inlays 10 according to three embodiments with a baffle element 20 extending inwards from the side portion 12. FIG. 8b, FIG. 8c refer to the same embodiment. FIG. 8d, FIG. 8e, FIG. 8f refer to the same embodiment.

    [0168] The inlay of FIG. 8a has a baffle element 20 with an inner contour, i.e., in a horizontal plane, having a rosette-shape. The depicted baffle element 20 has an inner contour according to an 8-leaved rosette. However, an inner contour according to a 3-, 4-, 5-, 6-, 7- or more leaved rosette is alternatively possible.

    [0169] The inlay 10 of FIG. 8b, FIG. 8c and the inlay 10 of FIG. 8d, FIG. 8c, FIG. 8f has a baffle element 20 with a shape of a screw extending along an inner surface of the side portion 12.

    [0170] The inlay 10 of FIG. 8b, FIG. 8c is adapted for a flask 30 with vertical inner side walls 34.

    [0171] The inlay 10 of FIG. 8d, FIG. 8e, FIG. 8f is adapted for an Erlenmeyer flask 30.

    [0172] FIG. 9a, FIG. 9b, FIG. 9c illustrate additional elements, such as a second opening 72, a recess 74, or a light-emitting diode 76, respectively.

    [0173] The bodies 10 depicted in FIG. 9a, FIG. 9b, FIG. 9c are similar to the inlays 10 described above, with a bottom portion 2, a side portion 12, and an intermediate portion 22. However, as compared to the respective inlays, the bodies 10 miss respective baffle elements. The bodies 10 may be combined with any of the baffle elements 20 described above, e.g., with a baffle element 20 from FIG. 7a, FIG. 7b, FIG. 7c, FIG. 7d, FIG. 7e, or from FIG. 8a, FIG. 8b, FIG. 8c, FIG. 8d, FIG. 8c, FIG. 8f to form an inlay 10. In other words, the additional elements of FIG. 9a, FIG. 9b, FIG. 9c (i.e., second opening 72, recess 74, or light-emitting diode 76) can be formed on the inlay 10 according to any of the embodiments described above.

    [0174] The body 10 of FIG. 9a is formed with (second) openings 72, with horizontal cross sections and orientations along the vertical directions, i.e., perpendicular to the cross section of the respective opening 72.

    [0175] When an inlay 10 with the body 10 of FIG. 9a is inserted into a flask 30, the openings 72 can be used to shine light through them into the inner volume 36 of the flask from below the flask 30, e.g., at an optode (e.g. for measuring pH and/or dissolved oxygen) arranged in the flask 30. Furthermore, the light can also directly shine into the solution when no optodes are applied in flask 30 to measure biomass concentration, particle concentration and/or particle size, fluorescent markers and/or particles or other optical signals. Therefore, the openings 72 permit in situ analysis of the solution in the flask 30.

    [0176] The body 10 of FIG. 9b is formed with recesses 74 for a feeding pill or a feeding cartridge, providing nutrients to the culture in flask 30 without the need for a pump. Alternatively, the recess may be used to provide a well-defined position for a buffer pill or a buffer cartridge for regulating the pH value of the solution.

    [0177] When an inlay 10 with the body 10 of FIG. 9b is inserted into a flask 30, the recesses 74 can be used to keep a feeding pill or a feeding cartridge at a controlled position with a well-defined local environment. Therefore, the recesses 74 permit a more reproducible cultivation of the microorganisms in the solution because feeding pills or discs do not aggregate/stick together due to the fluid movement (aggregation causes variable surface area and hence variable nutrient release via diffusion of nutrient into the fluid).

    [0178] The body 10 of FIG. 9c is formed with light-emitting diodes 76 mounted thereon.

    [0179] When an inlay 10 with the body 10 of FIG. 9c is inserted into a flask 30, the light-emitting diodes 76 permit cultivating phototrophic microorganisms, e.g. algae, providing a high light intensity directly to the fluid and hence optimized cultivation conditions in comparison to shake flasks that are illuminated traditionally from above or beneath the flask through the absorbing glass material of the flask.

    [0180] FIG. 10 compares the oxygen transfer rates OTR in mmol/(l*h) vs. time t in hours, for different flasks 30 with and without the inlay 10.

    [0181] Oxygen transfer rate 80 is the one of a regular Erlenmeyer flask, such as a narrow-neck Erlenmeyer flask according to ISO 1773 or a wide-neck Erlenmeyer flask according to ISO 24450, i.e. without any baffle or baffle element. The oxygen transfer rate 80 is the lowest one measured with approx. 25 mmol/(l*h).

    [0182] Oxygen transfer rate 82 is the one of a commercially available baffled flask. The oxygen transfer rate 82 is the highest one observed, thus providing a reference.

    [0183] Oxygen transfer rate 84 is the one of an Erlenmeyer flask, wherein a body 10 without a baffle element 20 is inserted. The oxygen transfer rate 84 is similarly low as the oxygen transfer rate 80 of the Erlenmeyer flask.

    [0184] Oxygen transfer rates 86, 88 are the ones of Erlenmeyer flasks, wherein a respective inlay 10 is inserted. More specifically, oxygen transfer rate 88 uses the inlay 10 of FIG. 7c, and oxygen transfer rate 86 uses the inlay 10 of FIG. 7e. The oxygen transfer rates 86, 88 are significantly improved as compared to the oxygen transfer rate 80 of the Erlenmeyer flask or to the oxygen transfer rate 84 of the body 10.

    [0185] To summarize FIG. 10, the inlay 10 with the baffle element 20 improves the oxygen transfer rate OTR to a degree similar to the one of a baffled flask.

    LIST OF REFERENCE SIGNS

    [0186] 10 inlay [0187] 30 flask [0188] 2 bottom portion of inlay [0189] 4 downward-facing or downward-and-outward-facing first surface [0190] 12 side portion of inlay [0191] 14 outer surface [0192] 22 intermediate portion of inlay [0193] 20 baffle element [0194] a reference line [0195] 18 inner angle [0196] c crease line [0197] 28 vertical plane [0198] t1 thickness of bottom portion [0199] t2 thickness of side portion [0200] 32 bottom inner surface of the flask [0201] 34 side inner surface of the flask [0202] 36 inner volume of the flask [0203] 16 opening [0204] 24 outward-facing surface of the intermediate portion of inlay [0205] r1 radius of opening [0206] W width of the ring-shaped bottom portion [0207] R radius of inlay [0208] H height of inlay [0209] A axis of flask [0210] 62 folding [0211] 64 aligning the crease line parallel to the axis of the flask [0212] 66 inserting the folded inlay into the flask [0213] 68 unfolding the inlay [0214] 70 c-shaped or half-moon-shaped element [0215] 72 second opening [0216] 74 recess [0217] 76 light emitting diode [0218] r oxygen-transfer rate (mmol/(l*h)) [0219] t time (hours)