COMPOSITION FOR WEIGHT REDUCTION AND BODY FAT REDUCTION, PREPARATION METHOD AND APPLICATION THEREOF

Abstract

A composition for weight reduction and body fat reduction, its preparation method and application are disclosed, which relates to the technical field of health food. The composition includes the following components: 10-30 parts of concentrated milk protein, 10-30 parts of soybean isolated protein powder, 10-30 parts of concentrated whey protein powder, 5-15 parts of skim milk powder, 0.5-15 parts of collagen peptide, 0.1-3 parts of Agaricus bisporus extract, 0.1-3 parts of Flammulina velutipes extract, 0.1-5 parts of okra powder, 0.1-2 parts of Opuntia ficus-indica extract, 1-10 parts of medium chain triglyceride microcapsule powder, 0.1-5 parts of mango concentrated powder, 0.1-2 parts of ginger oil microcapsule powder, 0.1-2 parts of citrus powder, 0.1-5 parts of guarana extract and 0.5-2 parts of compound vitamin.

Claims

1. A composition for weight reduction and body fat reduction, consisting of following components in parts by weight: 10-30 parts of concentrated milk protein, 10-30 parts of soybean isolated protein powder, 10-30 parts of concentrated whey protein powder, 5-15 parts of skim milk powder, 0.5-15 parts of collagen peptide, 0.5-3 parts of Agaricus bisporus extract, 0.5-3 parts of Flammulina velutipes extract, 0.5-5 parts of okra powder, 0.1-2 parts of Opuntia ficus-indica extract, 1-10 parts of medium chain triglyceride microcapsule powder, 0.5-5 parts of mango concentrated powder, 0.1-2 parts of ginger oil microcapsule powder, 0.1-2 parts of citrus powder, 0.5-5 parts of guarana extract and 0.5-2 parts of compound vitamin; a weight ratio of the Agaricus bisporus extract, the Flammulina velutipes extract, the okra powder and the Opuntia ficus-indica extract is 1-2:1-2:1-4:0.5-1.5; a weight ratio of the medium chain triglyceride microcapsule powder, the mango concentrated powder, the ginger oil microcapsule powder, the citrus powder and the guarana extract is 2-8:1-4:0.5-1.5:0.5-1.5:1-3; the compound vitamin comprises vitamin A, vitamin B1, vitamin B6, vitamin B12, vitamin C, vitamin E and vitamin D; Ginger oil microcapsule powder contains 10% gingerol.

2. The composition for weight reduction and body fat reduction of claim 1, wherein a weight ratio of the concentrated milk protein, the soybean isolated protein powder, the concentrated whey protein powder, the skim milk powder and the collagen peptide is 15-25:15-25:15-25:8-12: 3-12.

3. The composition for weight reduction and body fat reduction of claim 1, wherein a weight ratio of the concentrated milk protein, the soybean isolated protein powder, the concentrated whey protein powder, the skim milk powder and the collagen peptide is 20:20:20:12:8.

4. The composition for weight reduction and body fat reduction of claim 1, wherein a weight ratio of the Agaricus bisporus extract, the Flammulina velutipes extract, the okra powder and the Opuntia ficus-indica extract is 1:1:3:1.

5. The composition for weight reduction and body fat reduction of claim 1, wherein a weight ratio of the medium chain triglyceride microcapsule powder, the mango concentrated powder, the ginger oil microcapsule powder, the citrus powder and the guarana extract is 5:3:1:1:2.

6. The composition for weight reduction and body fat reduction of claim 1, wherein the composition consists of the following components in parts by weight: 15-25 parts of concentrated milk protein, 15-25 parts of soybean isolated protein powder, 15-25 parts of concentrated whey protein powder, 8-12 parts of skim milk powder, 3-12 parts of collagen peptide, 1-2 parts of Agaricus bisporus extract, 1-2 parts of Flammulina velutipes extract, 1-4 parts of okra powder, 0.5-1.5 parts of Opuntia ficus-indica extract, 2-8 parts of medium chain triglyceride microcapsule powder, 1-4 parts of mango concentrated powder, 0.5-1.5 parts of ginger oil microcapsule powder, 0.5-1.5 parts of citrus powder, 1-3 parts of guarana extract and 1-2 parts of compound vitamin.

7. The composition for weight reduction and body fat reduction of claim 6, wherein the composition consists of the following components in parts by weight: 20 parts of concentrated milk protein, 20 parts of soybean isolated protein powder, 20 parts of concentrated whey protein powder, 12 parts of skim milk powder, 8 parts of collagen peptide, 1 part of Agaricus bisporus extract, 1 part of Flammulina velutipes extract, 3 parts of okra powder, 1 part of Opuntia ficus-indica extract, 5 parts of medium chain triglyceride microcapsule powder, 3 parts of mango concentrated powder, 1 part of ginger oil microcapsule powder, 1 part of citrus powder, 2 parts of guarana extract and 2 parts of compound vitamin.

8. A method for preparing the composition for weight reduction and body fat reduction of claim 1, comprising the steps of: (1) mixing medium chain triglyceride microcapsule powder, mango concentrated powder, ginger oil microcapsule powder, citrus powder and guarana extract with the formulation dosage, adding water, and uniformly mixing to obtain a compound 1; (2) adding Agaricus bisporus extract, Flammulina velutipes extract, okra powder and Opuntia ficus-indica extract with the formulation dosage into the compound obtained in the step (1), and uniformly mixing to obtain a compound 2; (3) drying the compound 2 obtained in the step (2) to obtain a dried material; (4) mixing the dried material with concentrated milk protein, soybean isolated protein powder, concentrated whey protein powder, skim milk powder, collagen peptide and compound vitamin with the formulation dosage to obtain the composition for weight reduction and body fat reduction.

9. Use of the composition for weight reduction and body fat reduction of claim 1 in the preparing health products.

Description

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] The following non-limiting embodiments may allow one of ordinary skill in the art to more fully understand the disclosure without limiting the disclosure in any way. The following content is only exemplary description of the scope of protection of the present disclosure, and those skilled in the art can make various changes and modifications to the present disclosure according to the disclosed content, which should also belong to the scope of protection of the present disclosure as claimed.

[0032] The disclosure is further illustrated by means of the following specific embodiments. The various chemical reagents used in the embodiments of the present disclosure were obtained by conventional commercial means unless otherwise specified. Unless otherwise specified, the contents are mass contents in the following. Unless otherwise indicated, it is understood that it was carried out at room temperature.

[0033] In the following embodiments, the compound vitamin was a composition of vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C and vitamin E in a weight ratio of 1:3:1:1:100:10.

[0034] In the following embodiments, some raw material purchase information is as follows, but the present disclosure is not limited thereto:

TABLE-US-00001 Raw material Manufacturer Article No. Concentrated milk Fonterra 470 protein Soybean isolated Shandong Yuxin Biotechnology 787 protein powder Co., Ltd Concentrated whey Shanghai Proqin International PROTARMOR protein powder Trade Co., Ltd 80 Skim milk powder Hangzhou Juneng Food Co., Ltd / Collagen Peptide Jialida (Liaoyuan) Gelatin Co., Ltd Verisol B (CN) Agaricus bisporus Shanghai Ruixinda Health / extract Technology Co., Ltd Flammulina velutipes Beijing Xinda Qichuang Health / extract Technology Co., Ltd Okra powder Shanghai Yipan Biotechnology / Co., Ltd Opuntia ficus-indica Tianjin Binhai Jiecheng Specialty / extract Chemical Co., Ltd Medium chain Dalian Yinuo Biotechnology Co., / triglyceride Ltd microcapsule powder Mango concentrated Tianjin Binhai Jiecheng Specialty / powder Chemical Co., Ltd Ginger oil Xi'an Nuozhong Kangjian 10% Gingerol microcapsule powder Biotechnology Co., Ltd Citrus powder Guangzhou Mile Biotechnology / Co., Ltd Guarana Extract Hangzhou Tiancao Technology 10% Co., Ltd

[0035] Embodiments 1-7, comparative examples 1-6 were formulated as follows:

Embodiment 1

[0036] 20 parts of concentrated milk protein, 20 parts of soybean isolated protein powder, 20 parts of concentrated whey protein powder, 12 parts of skim milk powder, 8 parts of collagen peptide, 1 part of Agaricus bisporus extract, 1 part of Flammulina velutipes extract, 3 parts of okra powder, 1 part of Opuntia ficus-indica extract, 5 parts of medium chain triglyceride microcapsule powder, 3 parts of mango concentrated powder, 1 part of ginger oil microcapsule powder, 1 part of citrus powder, 2 parts of guarana extract and 2 parts of compound vitamin.

Embodiment 2

[0037] 15 parts of concentrated milk protein, 25 parts of soybean isolated protein powder, 15 parts of concentrated whey protein powder, 12 parts of skim milk powder, 3 parts of collagen peptide, 2 parts of Agaricus bisporus extract, 1 part of Flammulina velutipes extract, 4 parts of okra powder, 0.5 parts of Opuntia ficus-indica extract, 8 parts of medium chain triglyceride microcapsule powder, 1 part of mango concentrated powder, 1.5 parts of ginger oil microcapsule powder, 0.5 parts of citrus powder, 3 parts of guarana extract and 1 part of compound vitamin.

Embodiment 3

[0038] 25 parts of concentrated milk protein, 15 parts of soybean isolated protein powder, 25 parts of concentrated whey protein powder, 8 parts of skim milk powder, 12 parts of collagen peptide, 1 part of Agaricus bisporus extract, 2 parts of Flammulina velutipes extract, 1 part of okra powder, 1.5 parts of Opuntia ficus-indica extract, 2 parts of medium chain triglyceride microcapsule powder, 4 parts of mango concentrated powder, 0.5 parts of ginger oil microcapsule powder, 1.5 parts of citrus powder, 1 part of guarana extract and 2 parts of compound vitamin.

Embodiment 4

[0039] 10 parts of concentrated milk protein, 30 parts of soybean isolated protein powder, 10 parts of concentrated whey protein powder, 15 parts of skim milk powder, 0.5 parts of collagen peptide, 1 part of Agaricus bisporus extract, 1 part of Flammulina velutipes extract, 3 parts of okra powder, 1 part of Opuntia ficus-indica extract, 10 parts of medium chain triglyceride microcapsule powder, 0.5 parts of mango concentrated powder, 2 parts of ginger oil microcapsule powder, 0.1 parts of citrus powder, 5 parts of guarana extract and 0.5 parts of compound vitamin.

Embodiment 5

[0040] 10 parts of concentrated milk protein, 30 parts of soybean isolated protein powder, 10 parts of concentrated whey protein powder, 15 parts of skim milk powder, 0.5 parts of collagen peptide, 3 parts of Agaricus bisporus extract, 0.5 parts of Flammulina velutipes extract, 5 parts of okra powder, 0.1 parts of Opuntia ficus-indica extract, 5 parts of medium chain triglyceride microcapsule powder, 3 parts of mango concentrated powder, 1 part of ginger oil microcapsule powder, 1 part of citrus powder, 2 parts of guarana extract and 0.5 parts of compound vitamin.

Embodiment 6

[0041] 10 parts of concentrated milk protein, 30 parts of soybean isolated protein powder, 10 parts of concentrated whey protein powder, 15 parts of skim milk powder, 0.5 parts of collagen peptide, 3 parts of Agaricus bisporus extract, 0.5 parts of Flammulina velutipes extract, 5 parts of okra powder, 0.1 parts of Opuntia ficus-indica extract, 10 parts of medium chain triglyceride microcapsule powder, 0.5 parts of mango concentrated powder, 2 parts of ginger oil microcapsule powder, 0.1 parts of citrus powder, 5 parts of guarana extract and 0.5 parts of compound vitamin.

Embodiment 7

[0042] 30 parts of concentrated milk protein, 10 parts of soybean isolated protein powder, 30 parts of concentrated whey protein powder, 5 parts of skim milk powder, 15 parts of collagen peptide, 0.5 parts of Agaricus bisporus extract, 3 parts of Flammulina velutipes extract, 0.5 parts of okra powder, 2 parts of Opuntia ficus-indica extract, 1 part of medium chain triglyceride microcapsule powder, 5 parts of mango concentrated powder, 0.1 parts of ginger oil microcapsule powder, 2 parts of citrus powder, 0.5 parts of guarana extract and 2 parts of compound vitamin.

Comparative Example 1

[0043] The difference from embodiment 1 is that in the formulation of comparative example 1, no Agaricus bisporus extract was added, and the Flammulina velutipes extract was added in 2 parts, and the rest were the same.

Comparative Example 2

[0044] The difference from embodiment 1 is that in the formulation of comparative example 2, no Opuntia ficus-indica extract was added, and the Flammulina velutipes extract was added in 2 parts, and the rest were the same.

Comparative Example 3

[0045] The difference from embodiment 1 is that in the formulation of comparative example 3, no ginger oil microcapsule powder was added, and the medium chain triglyceride microcapsule powder was added in 6 parts, and the rest were the same.

Comparative Example 4

[0046] The difference from embodiment 1 is that the mango concentrated powder was replaced by the conjugated linoleic acid microcapsule powder in the same amount, and the rest were the same.

Comparative Example 5

[0047] 20 parts of concentrated milk protein, 20 parts of soybean isolated protein powder, 20 parts of concentrated whey protein powder, 12 parts of skim milk powder, 8 parts of collagen peptide, 4 parts of Agaricus bisporus extract, 4 parts of Flammulina velutipes extract, 0.3 parts of okra powder, 3 parts of Opuntia ficus-indica extract, 5 parts of medium chain triglyceride microcapsule powder, 3 parts of mango concentrated powder, 1 part of ginger oil microcapsule powder, 1 part of citrus powder, 2 parts of guarana extract and 2 parts of compound vitamin.

Comparative Example 6

[0048] 20 parts of concentrated milk protein, 20 parts of soybean isolated protein powder, 20 parts of concentrated whey protein powder, 12 parts of skim milk powder, 8 parts of collagen peptide, 1 part of Agaricus bisporus extract, 1 part of Flammulina velutipes extract, 3 parts of okra powder, 1 part of Opuntia ficus-indica extract, 12 parts of medium chain triglyceride microcapsule powder, 0.1 parts of mango concentrated powder, 3 parts of ginger oil microcapsule powder, 0.5 parts of citrus powder, 1 part of guarana extract and 2 parts of compound vitamin.

Preparation Example

[0049] Compositions of all embodiments and comparative examples were prepared according to the following method, in their respective formulations: [0050] (1) mixing medium chain triglyceride microcapsule powder, mango concentrated powder, ginger oil microcapsule powder, citrus powder and guarana extract with the formulation dosage, adding purified water, adjusting the solid content to 45%, stirring, and fully and uniformly mixing to obtain a compound 1; [0051] (2) adding Agaricus bisporus extract, Flammulina velutipes extract, okra powder and Opuntia ficus-indica extract with the formulation dosage into the compound obtained in the step (1), stirring, and fully and uniformly mixing to obtain a compound 2; [0052] (3) spray drying the compound 2 obtained in the step (2) to obtain a dried material; [0053] (4) fully mixing the dried material with concentrated milk protein, soybean isolated protein powder, concentrated whey protein powder, skim milk powder, collagen peptide and compound vitamin with the formulation dosage to obtain the composition for weight reduction and body fat reduction.

Result Detection

1. Animal Experiment

[0054] Experimental animals: male C57BL/6 mice, 4 weeks old;

[0055] Feeding conditions: mice were housed in plastic cages, with a 12/12 h light/dark cycle, free access to water and diet (tap water for drinking water, basic feed including corn, wheat, alfalfa, fish meal, soybean meal, chicken meal, vegetable oil, amino acids, vitamins, minerals, etc.), at temperature of 25 C. and air humidity of 40-60%, in a stable environment, with adaptive feeding.

[0056] Grouping: after one week of adaptive feeding, the mice were randomly divided into 15 groups with 10 mice in each group, wherein 13 groups were experimental groups and the compositions of embodiments 1 to 7 and comparative examples 1 to 6 were respectively given, one group was a model group and one group was a blank group.

[0057] Feeding mode: the blank group was continuously fed with the original basic feed, the model group and the experimental groups were fed with the high-fat feed (made by uniformly mixing the original basic feed, lard and egg yolk powder in a mass ratio of 7:2:1), the feeding amount was 80 g/kg (mouse weight), and the mice could drink water and move freely.

[0058] During the experiment, the experimental groups were given aqueous solutions of the compositions prepared in embodiments 1 to 7 and comparative examples 1 to 6 by gavage, respectively, where the amount of the composition was 500 mg/kg (mouse weight), and the normal group and the model group were given equal amounts of physiological saline by gavage at a volume of 10 mL/kg, and the gavage continued for 4 weeks. The initial weight average value M0 before the gavage of each group of mice and the weight average value M1 at the fourth week after the gavage were recorded, and the body fat was weighed by laparotomy after the experiment was completed.

[0059] The weights of the mice were weighed as follows:

TABLE-US-00002 Group M0 (g) M1 (g) Blank group 16.13 0.14 22.64 1.67 Model group 16.48 0.27 35.19 2.57**## Embodiment 1 group 16.34 0.16 22.84 1.06 Embodiment 2 group 15.86 0.15 24.21 1.03 Embodiment 3 group 15.90 0.18 23.90 0.96 Embodiment 4 group 16.27 0.09 24.73 1.47 Embodiment 5 group 15.94 0.11 24.55 2.12 Embodiment 6 group 16.07 0.14 24.62 1.68 Embodiment 7 group 16.22 0.20 25.34 2.04*# Comparative 16.31 0.24 31.38 2.37**## example 1 group Comparative 15.91 0.13 32.07 1.95**## example 2 group Comparative 16.16 0.19 30.22 2.37**## example 3 group Comparative 16.36 0.18 .sup.28.85 1.62*# example 4 group Comparative 16.09 0.11 30.34 2.34**# example 5 group Comparative 16.20 0.16 30.61 1.88**# example 6 group Note: compared with the blank group, *P < 0.05, **P < 0.01; compared with the model group, P < 0.05, P < 0.01; compared with the embodiment 1 group, #P < 0.05, ##P < 0.01.

[0060] Body fat weighing results were as follows:

TABLE-US-00003 Total body fat weight Group (g) Blank group 2.87 0.14 Model group 5.26 0.65**## Embodiment 1 group 2.76 0.31 Embodiment 2 group 2.91 0.28 Embodiment 3 group 2.85 0.36 Embodiment 4 group 2.96 0.17 Embodiment 5 group 2.92 0.29 Embodiment 6 group 3.15 0.31*.sup. Embodiment 7 group 3.22 0.17*.sup. Comparative 4.59 0.44**## example 1 group Comparative 4.71 0.32**## example 2 group Comparative .sup.4.05 0.17**# example 3 group Comparative 3.78 0.34**# example 4 group Comparative 4.32 0.36**## example 5 group Comparative 4.13 0.42*# example 6 group Note: compared with the blank group, *P < 0.05, **P < 0.01; compared with the model group, P < 0.05, P < 0.01; compared with embodiment 1 group, #P < 0.05, ##P < 0.01.

[0061] From the results in the tables, the formulations of embodiments 1 to 7 significantly inhibited weight gain in mice caused by a high fat diet, and the weight loss effect of embodiment 1 was best from the specific data presentation. Meanwhile, the body fat change result of the mice shows that the composition of the present disclosure has the effects of promoting fat metabolism and reducing the content of fat in the body.

[0062] The results of comparative examples 1 to 3 show that the weight reduction effect is poor without the addition of Agaricus bisporus extract, Opuntia ficus-indica extract or ginger oil microcapsule powder, indicating the interaction between the ingredients in the formulation, which further improves the weight reduction effect. In comparative example 4, the weight reduction effect of the conjugated linoleic acid microcapsule powder instead of the mango concentrated powder is obviously inferior to that of embodiment 1, which shows that the mango concentrated powder has better performance than the conjugated linoleic acid microcapsule powder in the formulation of the disclosure.

[0063] In comparative example 5, the weight reduction effect is obviously poor due to the change of the compounding ratio of the Agaricus bisporus extract, the Flammulina velutipes extract, the okra powder and the Opuntia ficus-indica extract, and in comparative example 6, the weight reduction effect is obviously poor due to the change of the compounding ratio of the medium chain triglyceride microcapsule powder, the mango concentrated powder, the ginger oil microcapsule powder, the citrus powder and the guarana extract, which means that the compounding of the Agaricus bisporus extract, the Flammulina velutipes extract, the okra powder and the Opuntia ficus-indica extract in a specific ratio plays the best role in blocking fat absorption and promoting fat metabolism, and the compounding of the medium chain triglyceride microcapsule powder, the mango concentrated powder, the ginger oil microcapsule powder, the citrus powder and the guarana extract in a specific ratio plays the best role in accelerating fat metabolism, which realizes the better weight reduction effect.

2. Volunteer Experiment

[0064] Twelve healthy adults, including six men and six women, were selected in each group. The age group was 20-35 years old, and the BMI was 24-32 kg/m.sup.2. They had no other metabolic diseases, digestive system diseases, endocrine system diseases and mental diseases, etc.; they had no history of allergies or intolerances to the food under test, no recent gastrointestinal diseases or taking any drugs. Subjects were trained prior to the experiment and informed consent was signed.

[0065] Control group 1: each subject ate normally according to the recommended daily nutrient intake recommended by the Chinese Nutrition Association, and drank 150 mL of warm water within 30 min before meals, once in the morning and once in the evening.

[0066] Experiment group 1: each subject ate normally according to the recommended daily nutrient intake recommended by the Chinese Nutrition Association, and ate 15 g of the composition provided in embodiment 1 (brewed with 150 mL warm water) within 30 min before meals, once in the morning and once in the evening.

[0067] Experiment group 2: each subject ate normally according to the recommended daily nutrient intake recommended by the Chinese Nutrition Association, and ate 15 g of the composition provided in comparative example 4 (brewed with 150 mL warm water) within 30 min before meals, once in the morning and once in the evening.

[0068] Test instrument: body composition analyzer, Jawon, model ioi353.

[0069] The data testing mode was as follows: {circle around (1)} Body weight and body fat: body weight and body fat were measured on the first morning after the start of the experiment and on the next morning after the end of the experiment following empty urine on an empty stomach, {circle around (2)} Satiety feeling: the satiety feeling was fed back 2 hours after breakfast and dinner, and the satiety feeling was recorded in four ways of hunger, general, better and good.

[0070] For 30 consecutive days, the body weight, body fat and satiety feeling of each group of subjects were detected and recorded, and the statistical results were as follows:

TABLE-US-00004 Average Average weight Average changes in satiety Group change body fat feeling Control group Increase by 0.4 kg Increase by 0.2 kg General 1 Experimental Reduce by 2.8 kg Reduce by 2.4 kg Better Group 1 Experimental Reduce by 1.3 kg Reduce by 1.5 kg Better Group 2

[0071] The results show that for overweight people, most of the them are in a fat-prone constitution, the products used in the experiment group 1 have good weight and body fat reducing effects under the same dietary structure, and the products in the experiment group 2 also have certain weight and fat reducing effects, but the effects are obviously inferior to those of the experiment group 1, so that the composition of the embodiment 1 is optimal.

[0072] The foregoing descriptions are merely exemplary embodiments of the present disclosure, but are not intended to limit the present disclosure. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present disclosure shall belong to the scope of protection of the present disclosure.