Single factor modeling-constraint method and device for mice chronic fatigue syndrome

Abstract

The present invention belongs to animal experiments field, disclosed a single factor modeling-constraint method and device for mice chronic fatigue syndrome, the modeling-constraint method is specifically using mineral water bottle to restraint mice; the mineral water bottle is divided into upper, middle and lower parts; the body of mineral water bottle is uniformly drilled for mice to breathe; the inner of the mineral water bottle is blocked by a cardboard to reduce the move space of mice; cover the mice through the divided bottle; one end of the opening is tightly attach to the wall, the other end is tightly attach to the mice feeding box, and keeping 30 minutes to restraint. The present invention utilizes mineral water bottle to make three simple restraint tube, not only saves the costs of experiment, but also turns waste into treasure, which is a simple operation, and increases the possibility of the experiment.

Claims

1. A single factor modeling-constraint device for mice chronic fatigue syndrome, the single factor modeling-constraint device for mice chronic fatigue syndrome using a mineral water bottle to restrain mice; the mineral water bottle is divided into upper, middle and lower parts, which are part A, part B and part C respectively to restrain mice; a body of the mineral water bottle is equipped with holes for mice to breathe; a height of part A, part B and part C of mineral water bottle are the same; an inner of part A, part B and part C which are divided by the body of mineral water bottle is individually blocked by a cardboard to reduce a moving space of mice; a baffle is set on a surface of the cardboard, the baffle is made from a special material including an active infrared alarm device, wherein the active infrared alarm device is a pyroelectric infrared sensor, wherein the active infrared alarm device is configured to actively emit infrared rays, set a restraint time for 30 minutes through a computer software, and after boot up a program; if the mineral water bottle leaves the baffle, the infrared rays bounce back when encountering obstacles and are detected by a probe of the active infrared alarm device and emits alarm to reduce the moving space of mice; the special material is a combination of LiTaO.sub.3, LATGS and PZT.

2. The single factor modeling-constraint device for mice chronic fatigue syndrome according to claim 1, wherein the cardboard is equipped with holes for mice to breathe, and a shape of the cardboard comprises quadrilateral, triangle, and rhombus.

3. The single factor modeling-constraint device for mice chronic fatigue syndrome according to claim 1, wherein the part A, part B and part C are respectively placed on a same horizontal plane, one end of the part A, part B and part C is attached to the obstacles, and the other end of the part A, part B and part C is attached to a mice feeding box.

4. The single factor modeling-constraint device for mice chronic fatigue syndrome according to claim 3, wherein the mice feeding box comprises box baffle, the box baffle is made from the same special material with the baffle used on the cardboard.

Description

BRIEF DESCRIPTION OF DRAWINGS

(1) FIG. 1 is a structural schematic diagram provided by embodiment 1 of the present invention;

(2) FIG. 2 is a structural schematic diagram provided by embodiment 2 of the present invention;

(3) FIG. 3 is a schematic diagram of the baffle structure provided by an embodiment of the present invention;

(4) FIG. 4 is a structural schematic diagram provided by embodiment 3 of the present invention;

(5) FIG. 5 is an experimental effect diagram of the application of an embodiment of the present invention.

DETAILED DESCRIPTION OF THE EMBODIMENTS

(6) To make the objectives, technical solutions and advantages of the present invention clearer, a detailed description will be given below, in combination with the embodiments. It should be understood that the specific embodiments are only used to explain the present invention, which are not used to limit the present invention.

(7) To make the person skilled in the art fully understand how to implement the present invention, this section provides an explanation and implementation example of the technical solutions of the claims.

Embodiment 1

(8) The embodiments of the present invention provide a single factor modeling-constraint method for mice chronic fatigue syndrome, which utilize mineral water bottle to restraint mice. The structure of the mineral water bottle as shown in FIG. 1:

(9) The mineral water bottle is divided into upper, middle and lower parts, which are part A, part B and part C respectively to restraint mice.

(10) The body of mineral water bottle is equipped with holes uniformly for mice to breathe.

(11) Further, the height of part A, part B and part C of mineral water bottle which in the first step are same.

Embodiment 2

(12) As shown in FIG. 2, the inner of part A, part B and part C which are divided by the body of mineral water bottle is blocked by the cardboard to reduce the moving space of mice.

(13) Further, the cardboard is equipped with holes for mice to breathe, and the cardboard can be formed into different shapes in the bottle, including quadrilateral, triangle, and rhombus.

Embodiment 3

(14) As shown in FIG. 4, the part A, part B and part C are respectively place on the same horizontal plane. One end of the part A, part B and part C is tightly attach to the wall, the other end is tightly attach to the mice feeding box.

(15) The wall and the mice feeding box use high-tech baffle. The route map of the infrared alarm that inside the baffle as shown in FIG. 3. The baffle is made of special material (the special material is a combination of LiTaO.sub.3, LATGS and PZT, which is on the piezoelectric crystal such as titanium zirconate, with electrodes on the upper and lower surfaces, and a black film is added to the surface. When infrared light is intermittently illuminated, the temperature of the surface is rising, which causing the change of the arrangement of atoms that inside the crystal, that is, resulting in spontaneous polarization charges. This phenomenon is called the pyroelectric effect, which is one of the thermoelectric effects. The surface charge generated by the pyroelectric effect is temporary, and as soon as it appears, it is quickly neutralized by various ions in the air).

(16) The embodiment of the present invention is equipped with active infrared alarm device, specifically pyroelectric infrared sensor, the alarm actively emits infrared rays, set the restraint time for 30 minutes through the computer software, and after boot up the program. If the bottle leaves the baffle, the infrared rays bounce back when encountering obstacles, which is received by the probe of the alarm and emits alarm to reduce the moving space of mice.

(17) The embodiment of the present invention also provides a single factor modeling-constraint device for mice chronic fatigue syndrome, includes: computer, cardboard, baffles and the mineral water bottle;

(18) Turn on the wireless cardboard and the baffle operation panel, and boot up the computer, set the operation parameters. Cover the mice through the opening of the segmented part of the mineral water bottle and make it touch the baffle as much as possible. Control the computer to start timing function, which is 30 minutes. After the timing starts, the computer controls the corresponding material in the baffle to tightly suck the plastic bottle to prevent mice to escape;

(19) During the restraint period, the baffle plays monitoring and alarm function, and can remote observe the restraint situation by computer. If the mouse breaks free, the baffle will promptly transmit the signal to computer to alarm. After 30 minutes of restraint is over, the computer receives signal, and to remind in time. At the same time, the corresponding material's function in the baffle is dismissed, which can depart the plastic bottle and the baffle.

(20) When using traditional mice restraint cages in the experiment, due to the limitation of human and financial resources, mice can easily break free from the restraint cage, which needs experimental person to watch all the time. Resulting in low experimental efficiency and inability to complete experimental tasks on time every day. After using the present invention, sufficient restraint cages can be made according to specific experimental tasks, which can complete the restraint tasks. During the restraint period, the experimental person can remotely control and observe the state of the mice, without worrying about the mice breaking out from the restraint cage. The experimental efficiency is greatly improved, and the experimental effect is good.

(21) From the previous experimental data, using the present invention to restraint the experimental group mice, the results of the eight arms experiment show a statistically significant difference compared with the control group, indicating that the restraint effect of the present invention is good.

(22) As shown in FIG. 5, it can be seen from the experimental results graph, except the second day, the eight arm experimental results of the control group were better than the experimental groups, and the total number of arm insertion is more than the experimental groups, indicating that using this method to model has good effect and is success to restraint.

(23) The described embodiments are only specific embodiments of the present invention, but the scope of the protection is not limited by the above-mentioned. Any person skilled in the art in the technical scope of the present invention disclosed, any modifications, equivalent replacements and improvements within the spirit and principles of the present invention should be involved in the scope of protection of the present invention.