Cosmetic Composition Containing Exosomes Extracted from Stem Cell for Skin Whitening, Antiwrinkle or Regeneration

Abstract

A cosmetic composition for skin whitening, wrinkle improvement or skin regeneration includes, as an active ingredient, exosomes derived from stem cells comprising proliferating stem cells.

Claims

1. A cosmetic composition for skin whitening, wrinkle improvement or skin regeneration comprising, as an active ingredient, exosomes derived from stem cells comprising proliferating stem cells.

2. The cosmetic composition of claim 1, wherein the stem cells comprise one or more of bone marrow stem cells, cord blood stem cells, and adipose-derived stem cells.

3. The cosmetic composition of claim 2, wherein the stem cells are human-derived, animal-derived or plant-derived stem cells.

4. The cosmetic composition of claim 1, wherein the exosomes are capable of skin whitening, wrinkle improvement or regeneration associated with corresponding target cells or a tissue after treatment of the corresponding target cells or the tissue using the exosomes at a concentration of 1 to 150 g per 1 mL of the cosmetic composition.

5. The cosmetic composition of claim 1, wherein the cosmetic is any one of formulations selected from the group consisting of skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutritive lotion, massage cream, nutritive cream, moisture cream, hand cream, foundation, essence, nutritive essence, face packs, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, cleanser, treatment, cosmetic solution, cosmetic packs, ointments, gels, liniments, liquids, patches and spray.

6. A method of improving migration of foreskin fibroblasts, the method comprising: preparing exosomes derived from stem cells comprising proliferating human adipose-derived stem cells; and treating the foreskin fibroblasts with the exosomes to improve the migration of the foreskin fibroblasts.

7. The method of claim 6, wherein the stem cells comprise one or more of bone marrow stem cells, cord blood stem cells, and adipose-derived stem cells.

8. The method of claim 7, wherein the stem cells are human-derived, animal-derived or plant-derived stem cells.

9. The method of claim 6, wherein the treating the foreskin fibroblasts with the exosomes comprises treating the foreskin fibroblasts with the exosomes at a concentration of 1 to 150 g per 1 mL of a composition comprising the exosomes.

10. A method of improving collagen synthesis of foreskin fibroblasts, the method comprising: preparing exosomes derived from stem cells comprising proliferating human adipose-derived stem cells; and treating the foreskin fibroblasts with the exosomes to improve the collagen synthesis of the foreskin fibroblasts.

11. The method of claim 10, wherein the stem cells comprise one or more of bone marrow stem cells, cord blood stem cells, and adipose-derived stem cells.

12. The method of claim 11, wherein the stem cells are human-derived, animal-derived or plant-derived stem cells.

13. The method of claim 10, wherein the treating the foreskin fibroblasts with the exosomes comprises treating the foreskin fibroblasts with the exosomes at a concentration of 1 to 150 g per 1 mL of a composition comprising the exosomes.

14. A method for skin whitening, wrinkle improvement or skin regeneration, the method comprising: preparing exosomes derived from stem cells comprising proliferating stem cells; administering the exosomes to a subject to whiten skin of the subject, improve wrinkles of the subject or regenerate skin of the subject, or a combination thereof

15. The method of claim 14, wherein the stem cells comprise one or more of bone marrow stem cells, cord blood stem cells, or adipose-derived stem cells.

16. The method of claim 15, wherein the stem cells are human- derived, animal-derived or plant-derived stem cells.

17. The method of claim 14, wherein the administering the exosomes to the subject comprises administering the exosomes to the subject at a concentration of 1 to 150 g per 1 mL of a composition comprising the exosomes.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0076] FIG. 1 is a schematic diagram of the exosomes derived from stem cells differentiating into adipocytes and the application thereof

[0077] FIG. 2 is a schematic diagram of the method for isolating exosomes from stem cells differentiating into adipocytes.

[0078] FIG. 3A to 3C shows diagrams illustrating the characteristics of the exosomes derived from stem cells differentiating into adipocytes; A: structure and shape of the exosomes (transmission electron microscope), B: size of the exosome (nanoparticle analyzer, dynamic light scattering), C: exosome membrane surface marker (Western Blot).

[0079] FIG. 4A to 4C shows diagrams illustrating lipid-related bioactive factors in the exosomes through a microarray; A: exosomes derived from proliferating stem cells (hASC-EXO), B: exosomes derived from stem cells differentiating into adipocytes (D-EXO), C: adipokine array map.

[0080] FIG. 5 shows a diagram illustrating the expression rate of factors affecting the differentiation into adipocytes; exosomes derived from proliferating stem cells (hASC-EXO) and exosomes derived from stem cells differentiating into adipocytes (D-EXO).

[0081] FIG. 6 shows the result of inducing differentiation of human adipose-derived stem cells into adipocytes; A: human adipose-derived stem cells (hASCs), B: positive control group (DM), exosomes derived from stem cells differentiating into adipocytes (D-EXO) and exosomes derived from proliferating stem cells (hASC-EXO).

[0082] FIG. 7 shows the result of Oil red O staining of stem cells induced to differentiate into adipocytes; A: human adipose-derived stem cells (hASCs), B: positive control group (DM), exosomes derived from stem cells differentiating into adipocytes (D-EXO) and exosomes derived from proliferating stem cells (hASC-EXO).

[0083] FIG. 8 shows the result of inducing the formation of adipose tissues for 3 weeks by carrying the exosomes in a hydrogel obtained by mixing collagen and methylcellulose and subcutaneously injecting the exosomes into nude mice models; A: collagen/methylcellulose hydrogel (Gel), B: hydrogel carrying the exosomes derived from proliferating stem cells (hASC-EXO), C: hydrogel carrying the exosomes derived from stem cells differentiating into adipocytes (D-EXO).

[0084] FIG. 9 shows the result of hematoxylin-eosin staining of the gels subcutaneously injected into nude mice models. One gel does not carry the exosome (Gel), and the other gels carry the exosomes derived from proliferating stem cells (hASC-EXO) and the exosomes derived from stem cells differentiating into adipocytes (D-EXO), respectively. A, C, E: 40 magnification; B, D, F: 100 magnification.

[0085] FIG. 10 shows the result of Oil red O staining of the gels subcutaneously injected into nude mice models. One gel does not carry the exosome (Gel), and the other gels carry the exosomes derived from proliferating stem cells (hASC-EXO) and the exosomes derived from stem cells differentiating into adipocytes (D-EXO), respectively.

[0086] FIG. 11 is a schematic diagram of the method of isolating exosomes from proliferating human adipose-derived stem cells.

[0087] FIG. 12 shows images of human adipose-derived stem cells, human epidermal keratinocytes, and human foreskin fibroblasts observed with a microscope.

[0088] FIG. 13A to 13C shows diagrams illustrating the characteristics of the exosomes derived from human adipose-derived stem cells (Stem-Exo). The exosomes derived from human keratinocytes (K-Exo) and from human foreskin fibroblasts (F-Exo) were used as control groups. The structure and shape of the exosomes (transmission electron microscope), and the size of the exosomes (nanoparticle analyzer, dynamic light scattering) were illustrated respectively; A: Stem-Exo (scale bars indicate 50 nm (black), 100 nm (white), respectively), B: K-Exo (scale bars indicate 50 nm (black), 100 nm (white), respectively) and C: F-Exo (scale bars indicate 50 nm (black) and 200 nm (white), respectively).

[0089] FIG. 14A to 14D shows diagrams showing a comparison of the expression levels of bioactive factors contained in the exosomes derived from human adipose-derived stem cells (Stem-EXO), the exosomes derived from human epidermal keratinocytes (K-EXO) and the exosomes derived from human fibroblasts (F-EXO) using a microarray; A: table of microarray, B: result of microarray, C and D: graph showing the relative expression levels of bioactive factors.

[0090] FIG. 15A to 15I shows graphs illustrating the expression level of bioactive factor (A: PDGF-AA, B: PDGF-AB, C: PDGF-BB, D: FGF-6, E: MCP-F: MCP-3, G: Eotaxin, H: CCL-5, I: TIMP-1) related to wrinkle improvement effect in the exosomes using a microarray; Stem-EXO: exosomes derived from proliferating human adipose-derived stem cells, K-EXO: exosomes derived from human epidermal keratinocytes, F-EXO: exosomes derived from human foreskin fibroblasts.

[0091] FIG. 16A to 16D shows graphs illustrating the expression level of bioactive factor (A: TGF-beta, B: TNF-alpha, C: IL-6, D: IL-8) related to whitening effect in the exosomes using g a microarray; Stem-EXO: exosomes derived from proliferating human adipose-derived stem cells, K-EXO: exosomes derived from human epidermal keratinocytes, F-EXO: exosomes derived from human foreskin fibroblasts.

[0092] FIG. 17A to 17F shows graphs illustrating the expression levels of bioactive factors (A: EGF, B: HGF, C: PAI-1, D: VEGF, E: Angiogenin, F: Angiopoietin-1) related to skin regeneration and angiogenesis in the exosomes using a microarray; Stem-EXO: exosomes derived from proliferating human adipose-derived stem cells, K-EXO: exosomes derived from human epidermal keratinocytes, F-EXO: exosomes derived from human foreskin fibroblasts.

[0093] FIG. 18A to 18B shows diagrams illustrating the effect of human adipose-derived stem cell exosomes (Stem-EXO) on the migration of human fibroblasts; GM: stem cell culture medium (growth medium), SFM: serum-free medium, Stem-EXO: exosomes derived from proliferating human adipose-derived stem cells, K-EXO: exosomes derived from human epidermal keratinocytes, F-EXO: exosomes derived from human foreskin fibroblasts.

[0094] FIG. 19 shows a graph illustrating the effect of human adipose-derived stem cell exosomes (Stem-EXO) on collagen synthesis of human fibroblasts; SFM: serum-free medium, Stem-EXO: exosomes derived from proliferating human adipose-derived stem cells, K-EXO: exosomes derived from human epidermal keratinocytes, F-EXO: exosomes derived from human foreskin fibroblasts.

[0095] FIG. 20 shows a graph illustrating the effect of human adipose-derived stem cell exosomes (Stem-EXO) on the melanin synthesis of mouse melanocytes; GM: stem cell culture medium (growth medium), SFM: serum-free medium, Stem-EXO: exosomes derived from proliferating human adipose-derived stem cells, K-EXO: exosomes derived from human epidermal keratinocytes, F-EXO: exosomes derived from human foreskin fibroblasts.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0096] One aspect of the present invention is to provide a composition for inducing differentiation into adipocytes or regenerating adipose tissues. The composition may include, as an active ingredient, an exosome derived from stem cells differentiating into adipocytes, or an exosome derived from proliferating stem cells.

[0097] Another aspect of the present invention is to provide a cosmetic composition comprising a composition for inducing differentiation into adipocytes or regenerating adipose tissues. The cosmetic composition may include, as an active ingredient, an exosome derived from stem cells differentiating into adipocytes.

[0098] Still another aspect of the present invention is to provide a medium composition for inducing differentiation into adipocytes or regenerating adipose tissues. The medium composition may include, as an active ingredient, an exosome derived from stem cells differentiating into adipocytes.

[0099] Further another aspect of the present invention is to provide an injectable preparation comprising a composition for inducing differentiation into adipocytes or regenerating adipose tissues. The injectable preparation may include, as an active ingredient, an exosome derived from stem cells differentiating into adipocytes, and a hydrogel.

[0100] Still further another aspect of the present invention is to provide a cosmetic composition for skin whitening, wrinkle improvement or skin regeneration. The cosmetic composition may include, as an active ingredient, an exosome derived from proliferating stem cells.

[0101] Hereinafter, preferred embodiments are provided to help understanding of the present invention, but the embodiments are only for illustrative purposes. Further, it would be apparent to those skilled in the art that various modifications and alternative forms can be made within the scope and technical idea of the present invention, and that such modifications and alternative forms fall within the scope of the invention.

[0102] Some embodiments further relate to a method of inducing stem cells differentiating into adipocytes. For example, the method may include preparing exosomes derived from the stem cells differentiating into adipocytes and treating stem cells with the exosomes for a predetermined time period.

[0103] Some embodiments further relate to a method of inducing adipose tissue regeneration using exosomes. For example, the method may include preparing exosomes derived from the stem cells differentiating into adipocytes, placing the exosomes in a supporting material, and administering the supporting material to a subject, thereby regenerating adipose tissues. In some embodiments, the supporting material includes a hydrogel.

[0104] Some embodiments further relate to a method of producing a composition containing exosomes. For example, the method may include preparing exosomes derived from the stem cells differentiating into adipocytes and mixing the exosomes with any least one of stearic acid, cetyl alcohol, lanolin alcohol, liquid paraffin, Cyclomethicone, polyoxyethylene monoolein acid ester hexanediol, glycerin, triethylamine, or carbomer.

[0105] Some embodiments further relate to a method of improving migration of foreskin fibroblasts. For example, the method may include preparing exosomes derived from proliferating human adipose-derived stem cells and treating the foreskin fibroblasts with the exosomes, thereby improving the migration of the foreskin fibroblasts.

[0106] Some embodiments further relate to a method of improving collagen synthesis of foreskin fibroblasts. For example, the method may include preparing exosomes derived from proliferating human adipose-derived stem cells and treating the foreskin fibroblasts with the exosomes, thereby improving the collagen synthesis of the fore-skin fibroblasts.

Example 1

ExosomesDerived From Stem Cells Differentiating Into Adipocytes

Example 1-1

Isolation of Exosomes

[0107] In order to isolate the exosomes from stem cells differentiating into adipocytes, the differentiation into adipocytes was induced by culturing the stem cells in a differentiation medium.

[0108] The differentiation into adipocytes was confirmed as lipid droplets were formed in the cytoplasm while the stem cells became gradually uneven. The differentiating stem cell culture media were replaced with a serum-free medium and maintained for 48 hours, and the cell culture supernatant was recovered. The recovered cell culture supernatant was centrifuged at 300g for 10 minutes to remove the cells, and then centrifuged at 2,000g for 30 minutes to remove the cell secretion.

[0109] Thereafter, the cells were concentrated by centrifugation at 5,000g for 60 minutes using a centrifuge tube (molecular weight cut off=3,000, amicon tube) equipped with a filter having a molecular weight of 3,000. The supernatant obtained after the concentration was mixed with an exosome isolation reagent at a ratio of 1:0.5 and stored at 4 C. for one day. Subsequently, the cells were centrifuged at 10,000g for 60 minutes to obtain an exosome precipitate, then filtered through a filter (exosome spin column) having a molecular weight of 3,000, and washed with phosphate-buffered saline (PBS). The washed exosome precipitate was centrifuged at 10,000g for 60 minutes and resuspended in PBS (FIG. 2).

Example 1-2

Microscopic Analysis of Exosomes

[0110] The size and shape of the exosomes derived from Example 1-1 were confirmed using a transmission electron microscope and dynamic light scattering, and the surface protein of the exosomes was confirmed using Western Blot which detects a specific protein to the membrane surface of exosomes.

[0111] As a result, the exosomes isolated as shown in FIG. 3A were confirmed by a transmission electron microscope, and the size thereof was confirmed to be about 50.75 to 58.77 nm on average as shown in FIG. 3B. In addition, as shown in FIG. 3C, an exosome-specific marker expressed on the surface of the exosome membrane was confirmed through an antibody reaction.

Example 1-3

Analysis of Proteins and Bioactive Factors Related to Adipocyte Differentiation in Exosomes

[0112] A microarray was used to analyze the lipid-related bioactive factors present in the exosomes derived from stem cells differentiating into adipocytes and the exosomes derived from proliferating stem cells. The microarray was carried out through an antigen-antibody reaction, and the degree of fluorescence (Streptavidin-Cy3) expression was measured using a laser scanner (GenePix 4000B).

[0113] In addition, macrophage colony stimulating factor (MCSF), tumor necrosis factor- (TNF-), leptin, insulin, angiopoietinl (ANGPT1), and adipocyte complement-related protein of 30 kDa (Acrp30), all of which are bioactive factors influencing the differentiation into adipocytes among the factors expressed in the microarray analysis, were confirmed, and in this regard, the relative expression levels thereof in the exosomes derived from the stem cells differentiating into adipocytes and from proliferating stem cells were compared.

[0114] As a result, as shown in FIGS. 4A to 4C and Table 1, it was confirmed that there are different types of lipid-related bioactive factors present in the exosomes derived from the proliferating stem cells (hASC-EXO) and the exosomes derived from the stem cells differentiating into adipocytes (D-EXO), and it was also confirmed that there was a significant difference in the expression levels of bioactive factors affecting differentiation into adipocytes (FIG. 5).

TABLE-US-00001 TABLE 1 hASC-EXO D-EXO Adipsin ACRP30* OPG CRP ANGPT1* PDGF-AB Fas ANGPTL4* SDF-1 IL-1 sRI IL-1R4/ST2 TECK IL-6 IL-10 TGF-* MCP-1 Insulin* TIMP2 MCP-3 Leptin* TNF-* PDGF-BB MCSF* XEDAR

Example 1-4

Induction of Adipocyte Differentiation Using Exosomes

[0115] In order to induce adipocyte differentiation of stem cells using the exosomes, medium compositions each containing the exosomes derived from proliferating stem cell culture medium and the exosomes derived from stem cells differentiating into adipocytes were used. The medium compositions were used by adding the exosomes to the stem cell culture medium at a concentration of 30, 50 and 100 g/mL. After treating each medium composition on the cultured human adipose-derived stem cells (hASCs), the medium compositions were replaced once in every 3 days for 14 days.

[0116] The stem cells cultured in Dulbecco's Modified Eagle's Medium High Glucose medium (DMEM) containing 5% fetal bovine serum, 1 M dexamethasone, 1 g/mL insulin, 100 M indomethacin, 0.5 mM 3-isobutyl-1-methylxanthine were used as a positive control group. The stem cells treated with the exosomes derived from proliferating stem cells were used as a positive control group. Then, the cell shape and whether the differentiation was carried out were analyzed with respect to the stem cells, in which the differentiation into adipocytes was induced, using a microscope and Oil-red O staining for 14 days.

[0117] As a result, when the exosomes derived from the stem cells differentiating into adipocytes (D-EXO) were used, the stem cells were differentiated into adipocytes at a level similar to that of the positive control (DM) on day 7 (FIG. 6), and accordingly, the production of oil was confirmed (FIG. 7). However, in the case of the stem cells treated with the exosomes derived from proliferating stem cells (hASC-EXO), it was confirmed that only proliferation was carried out without differentiation into adipocytes

Example 1-5

Cosmetic Composition Comprising Exosomes Derived From Stem Cells Differentiating Into Adipocytes

[0118] According to Example 1-1, a liposome encapsulating the exosomes -derived from stem cells differentiating into adipocytes was prepared. Specifically, 3% by weight of lecithin was dispersed in an aqueous phase containing 0.01% by weight of the exosomes derived from stem cells differentiating into adipocytes at room temperature (15 C.), and then a reverse micelle emulsion (water/low temperature process carbon dioxide) was prepared using supercritical carbon dioxide. Subsequently, the reaction was terminated, the supercritical carbon dioxide was vaporized under reduced pressure to remove the supercritical carbon dioxide phase, and a low temperature process liposome suspension, in which the exosomes derived from stem cells differentiating into adipocytes are encapsulated, was obtained. Here, the temperature of the reaction process was 4 C. or below.

[0119] The cosmetic composition was prepared by the composition shown in Table 2 below using the liposome encapsulating the exosomes.

TABLE-US-00002 TABLE 2 Content Composition (% by weight) Stearic acid 2 Cetyl alcohol 2 Lanolin alcohol 2 Liquid paraffin 7 Cyclomethicone 5 Polyoxyethylene monooleic acid ester 2 Hexanediol 2 Glycerin 3 Triethylamine 5 Carbomer 0.2 Liposome encapsulating the exosomes 0.01 according to Example 1-1 of the present invention Purified water remainder

Example 1-6

Induction of Adipose Tissue Regeneration Using Exosomes Derived Rrom Stem Cells Differentiating Into Adipocytes

[0120] In order to confirm the effect on the adipose tissue regeneration when the exosomes derived from stem cells differentiating into adipocytes were injected into the body, the exosomes derived from the proliferating stem cells and the exosomes derived from stem cells differentiating into adipocytes were independently carried in a collagen/methylcellulose hydrogel.

[0121] Specifically, the hydrogel was prepared by adding methylcellulose powder to a collagen solution to form the collagen/methylcellulose hydrogel. That is, methylcellulose powder was added to a collagen solution dissolved in 0.02 N acetic acid at a concentration of 3 mg/mL such that the final concentration of methylcellulose became 6% by weight, and then the mixture was stirred at 4 C. for 1 hour to prepare the gel. In the thus-prepared collagen/methylcellulose hydrogel, the exosomes derived from the proliferating stem cells or the exosomes derived from stem cells differentiating into adipocytes were carried. Specifically, the exosomes were carried in the collagen/methylcellulose hydrogel to a final concentration of 50 g/mL, and then dispersed in the hydrogel by pipetting. Further, the hydrogel containing the exosomes was subcutaneously injected into the nude mice and observed for 3 weeks. The hydrogel containing no exosome was used as a negative control group, and the hydrogel containing the exosomes derived from proliferating stem cells (hASC-EXO) was used as a positive control group (FIG. 8). Three weeks later, hematoxylin-eosin staining and oil red o staining were performed to confirm the regeneration of adipose tissues in the transplanted hydrogel.

[0122] As a result, a large amount of mouse cells was introduced into the gel containing the exosomes derived from the stem cells differentiating into adipocytes (D-EXO) (FIG. 9), and a large number of adipocytes in which oil was produced were observed (FIG. 10), as compared with the negative and positive control groups. From these results, it can be concluded that the exosomes derived from stem cells differentiating into adipocytes or the collagen/methylcellulose hydrogel carrying the exosomes were remarkably effective in inducing the regeneration of adipose tissues.

Example 2

Exosomes Derived From Proliferating Stem Cells

Example 2-1

Isolation of Exosomes From Proliferating Human Adipose-Derived Stem Cells

[0123] The exosomes were isolated during the proliferation of human adipose-derived stem cells up to passages 7. That is, the exosomes were isolated from the proliferating human adipose-derived stem cells.

[0124] Specifically, the human adipose tissue-derived stem cells (passages 3 to 7) were cultured in a normal culture medium (Dulbecco Modified Eagle Medium (DMEM) containing 10% fetal bovine serum, 1% penicillin/streptomycin). Then, at 24 hours before isolating the exosomes, the cell culture media were replaced with DMEM medium, which is a serum-free and non-antibiotic medium without phenol red, and then maintained for 24 hours. After 24 hours, the cell culture supernatant was recovered. The recovered cell culture supernatant was centrifuged at 300g for 10 minutes to remove the cells, and then centrifuged at 2,000g for 30 minutes to remove the cell secretion. Thereafter, the cells were concentrated by centrifugation at 5,000g for 60 minutes using a centrifuge tube equipped with a filter having a molecular weight of 3,000 (molecular weight cut off=3,000, amicon tube). The supernatant obtained after the concentration was mixed with an exosome isolation reagent at a ratio of 1:0.5 and stored at 4 C. for one day. An exosome precipitate was obtained by centrifugation at 10,000g for 60 minutes, then filtered through a 0.22 m filter (exosome spin column) and washed with phosphate-buffered saline (PBS). The washed exosome precipitate was centrifuged at 10,000g for 60 minutes and resuspended in PBS (FIG. 11). After recovering the supernatant, the normal culture medium was added to the stem cells and cultured. This procedure was repeated up to passages 7 of the stem cells. The exosomes isolated during the process of proliferating up to passages 7 were used in the following experiments. In order to compare with the efficacy of the exosomes from the human adipose-derived stem cells, the exosomes were isolated from human epidermal keratinocytes and human foreskin fibroblasts in the same manner by the above method (FIG. 12).

Example 2-2

Microscopic Analysis of Exosomes

[0125] The sizes and shapes of the exosomes derived from human adipose-derived stem cells (Stem-Exo), the exosomes derived from human epidermal keratinocytes (K-Exo) and the exosomes derived from human foreskin fibroblasts (F-EXO) of Example 2-1 were confirmed by using a transmission electron microscope and dynamic light scattering.

[0126] As a result, the shape of each derived exosome was confirmed by a transmission electron microscope. In addition, the sizes of the exosomes derived from human adipose-derived stem cells, the exosomes derived from human epidermal keratinocytes, and the exosomes derived from human foreskin fibroblasts were about 69 nm, about 79.7 nm and about 94.6 nm, respectively, and the size of the exosomes (Stem-Exo) derived from the human adipose-derived stem cells was the smallest (FIGS. 13A to 13C).

Example 2-3

Analysis of Proteins and Bioactive Factors Associated With Wrinkle Improvement, Whitening and Skin Regeneration in Exosomes

[0127] A microarray analysis was performed to compare and analyze the bioactive factors associated with wrinkle improvement, whitening and skin regeneration present in the exosomes derived from human adipose-derived stem cells (Stem-Exo), exosomes derived from human epidermal keratinocytes (K-Exo) and exosomes derived from human foreskin fibroblasts (F-EXO). The microarray analysis was carried out through an antigen-antibody reaction, and the degree of fluorescence (Streptavidin-Cy3) expression was measured using a laser scanner (GenePix 4000B).

[0128] Through the microarray analysis, 9 bioactive factors influencing wrinkle improvement (PDFG-AA, PDGG-AB, PDGF-BB, FGF-6, MCP-1, MCP-3, Eotaxin, CCL-5, TIMP-1), 4 whitening-related bioactive factors (TGF-beta, TNF-alpha, IL-6, IL-8) and 6 bioactive factors related to skin regeneration and angiogenesis (EGF, HGF, PAI-1, VEGF, Angiogenin, Angiopoietin-1) were confirmed and in this regard, the relative expression levels of each bioactive factor in the exosomes derived from human adipose-derived stem cells and the exosomes derived from human epidermal keratinocytes and from human foreskin fibroblasts were compared (FIG. 14). FIGS. 14C and 14D show the relative expression levels of the bioactive factors, and the horizontal axis indicates the exosomes from human adipose-derived stem cells and the vertical axis indicates the exosomes from epidermal keratinocytes and fibroblast exosomes, respectively. In addition, the top line and the bottom line with the middle line of the graph at the center show 1.5-fold increase/decrease relative to the reference value, respectively. FIGS. 15A to 15I show the bioactive factors related to wrinkle improvement effect of the exosomes, FIGS. 16A to 16D show the bioactive factors related to whitening effect of the exosomes, and FIGS. 17A to 17F show the bioactive factors related to skin regeneration and angiogenesis of the exosomes.

[0129] As a result, as shown in FIGS. 15, 16 and 17, it was confirmed that there are different types of bioactive factors present in the exosomes derived from human adipose-derived stem cells (Stem-Exo), the exosomes derived from human epidermal keratinocytes (K-Exo) and the exosomes derived from human foreskin fibroblasts (F-EXO). Specifically, it was confirmed that the monocyte chemoattractant protein-1, -3 (MCP-1, -3), chemokine ligand 5 (CCL-5) and collagenase inhibitor (the tissue inhibitor of metalloproteinase-1 (TIMP-1)) related to the mechanisms associated with promoting collagen synthesis and inhibiting the degradation thereof, interleukin-6, -8 (IL-6, -8) associated with whitening, hepatocyte growth factor (HGF), palsminogen activator inhibitor-1 (PAI-1), angiogenin and angiopoietin-1 associated with skin regeneration and angiogenesis were over-expressed in the exosomes derived from human adipose-derived stem cells (Stem-EXO) compared to K-EXO and/or F-EXO (FIGS. 15, 16 and 17).

Example 2-4

Effect on Migration Effect of Human Foreskin Fibroblasts Using Exosomes Derived From Proliferating Human Adipose-Derived Stem Cells

[0130] In order to examine the effect of the exosomes derived from human adipose-derived stem cells on the migration of human foreskin fibroblasts, medium compositions each containing the exosomes derived from the proliferating human adipose-derived stem cell (Stem-EXO), the exosomes derived from human epidermal keratinocytes (K-EXO) and the exosomes extracted from human foreskin fibroblasts (F-EXO) were used. Each of the medium compositions was prepared by adding Stem-EXO to a DMEM serum-free culture medium at concentrations of 10, 30 and 50 g/mL, and adding K-EXO and F-EXO to a DMEM serum-free culture medium at a concentration of 50 g/mL, respectively. The DMEM medium containing 10% serum was used as a positive control group and the DMEM serum-free medium was used as a negative control group. The human foreskin fibroblasts were labeled with green fluorescent dye, then seeded in a 24-well plate at 110.sup.5 cells/well and cultured in a culture medium (DMEM containing 10% fetal bovine serum, 1% penicillin/streptomycin) for 72 hours. After culturing, an artificially uniform interval of wounds was prepared at the center of the bottom of the plate to which the cells were adhered using a sterilized yellow tip, and the medium compositions each containing the exosomes was applied to the cells.

[0131] As a result, the cells treated with the medium containing Stem-EXO at 24 hours showed a higher degree of migration than the cells treated with negative control, K-EXO and F-EXO, and such tendency was even more prominent in the medium containing Stem-EXO at a concentration of 30 and 50 g/mL. After 48 hours, the migration rapidly took place in the medium containing 10, 30 and 50 g/mL of Stem-EXO, showing better (e.g., faster, having less scaring, or less discoloration) wound healing effect compared to the media containing K-EXO and F-EXO (FIGS. 18A and 18B).

[0132] Therefore, the exosomes derived from human adipose-derived stem cells (Stem-Exo) showed an excellent effect on the migration of human foreskin fibroblasts compared to K-EXO or F-EXO.

Example 2-5

Effect of Exosomes Derived From Proliferating Human Adipose-Derived Stem Cells on Wrinkle Improvement

[0133] In order to examine the effect of the exosomes derived from human adipose-derived stem cells on the collagen synthesis of human foreskin fibroblasts, medium compositions each containing the exosomes derived from the proliferating human adipose-derived stem (Stem-EXO), the exosomes derived from human epidermal keratinocytes (K-EXO) and the exosomes derived from human foreskin fibroblasts (F-EXO) were used. The medium compositions each was prepared by adding Stem-EXO to a DMEM serum-free culture medium at concentrations of 10, 30 and 50 g/mL, and adding K-EXO and F-EXO to a DMEM serum-free culture medium at a concentration of 50 g/mL, respectively. The DMEM serum-free medium was used as a negative control group. The human foreskin fibroblasts were seeded in a 48-well plate at 510.sup.4 cells/well and cultured in a culture medium (DMEM containing 10% fetal bovine serum, 1% penicillin/streptomycin) for 72 hours and then washed with PBS, and the medium compositions each containing the exosomes was applied to the cells.

[0134] After completion of culturing, the culture solution of each well was recovered, centrifuged at 25 C. at 3,000 rpm for 10 minutes, and then the supernatant was taken and used for the extraction and quantification of soluble collagen. Each well of the plate from which the culture solution had been removed was washed with PBS. Then the cells were separated from the bottom of each well by applying trypsin (trypsin-EDTA), and the number of cells was measured.

[0135] Sircol collagen assay kit (Biocolor, UK) was used for the quantification of soluble collagen. The thus-obtained supernatant was treated with Tris-HC1 (pH 7.6) buffer mixed with polyethylene glycol and maintained at 4 C. for 12 hours or more. Thereafter, the resultant was centrifuged at 12,000 rpm for 10 minutes to concentrate collagen. After removing the supernatant, 1 mL of the provided collagen adsorption dye (sircol dye reagent) was added to the collagen pellet and then cultured with shaking for 30 minutes. The unadsorbed dye was removed by centrifugation at 12,000 rpm for 10 minutes, and the pellet was washed with an acid salt buffer. Then the dye adsorbed on the collagen was dissolved by applying an alkali reagent, and the absorbance was measured at a wavelength of 555 nm. The absorbance was substituted into the equation of the standard curve to calculate the amount of soluble collagen in the wells to which Stem-EXO, K-EXO, F-EXO and the negative control substance were added, respectively. The calibrated amount of collagen was substituted into the equation to calculate the synthesis rate.

[0136] As a result, the soluble collagen synthesis rate for the group treated with Stem-EXO increased depending on the concentration of the exosome used, compared with the negative control group (0.138 g). Specifically, in the case of the group treated with Stem-EXO at 50 g/mL, the amount of collagen synthesis was 2.59 g, which was significantly increased compared to the same amount of K-EXO (1.4 g) or F-EXO (0.8 g). Therefore, it can be implied that the exosomes derived from human adipose-derived stem cells has the effect of promoting collagen synthesis of human foreskin fibroblasts (FIG. 19).

Example 2-6

Inhibitory Effect of Exosomes Derived From Proliferating Human Adipose-Derived Stem Cells on Melanin Production Using Mouse Melanoma

[0137] The whitening effect of the exosomes derived from human adipose-derived stem cells (Stem-EXO), and the exosomes derived from human epidermal keratinocytes (K-EXO) and the exosomes derived from human foreskin fibroblasts (F-EXO) as control groups were determined by the degree of inhibition of melanin production in mouse melanoma. The melanoma cells are cells that are derived from mouse melanoma and secrete a melanin pigment referred to as melanin The melanoma cells were seeded at a density of 110.sup.5 cells/well in a 96-well plate to adhere the cells, and then cultured for 3 days by replacing the culture medium with a medium containing Stem-EXO, K-EXO and F-EXO, respectively. After 3 days, the medium was recovered and centrifuged at 4,500 rpm for 10 minutes, and the absorbance was measured at 405 nm to calculate the amount of melanin released from the cells. The cells adhered to the plate were removed by applying trypsin (trypsin-EDTA), and the number of cells was measured, followed by centrifugation to recover the cells. The cells were washed once with PBS and centrifuged to obtain cell pellets. To the cell pellets, 1 ml of 1 N sodium hydroxide (NaOH) solution containing 10% dimethyl sulfoxide (DMSO) was added to dissolve the melanin at 80 C. for 2 hours, then the resultant was added to a 96-well plate, and the absorbance was measured at 405 nm. The melanin was quantified using the measured absorbance and normalized to the protein concentration of the sample to determine the concentration of the synthesized melanin.

[0138] The exosomes derived from human adipose-derived stem cells were used on the melanoma cells at a concentration of 10, 30 and 50 g/mL, and the degree of melanin synthesis was examined. As a result, it was confirmed that the melanin synthesis was reduced at all concentrations of the exosomes derived from stem cells (FIG. 20).

Example 2-7

Cosmetic Compositions Containing Exosomes Derived From Proliferating Human Adipose-Derived Stem Cells

[0139] According to Example 2-1, a liposome encapsulating the exosomes and derived from the proliferating human adipose-derived stem cells was prepared.

[0140] Specifically, 3% by weight of lecithin was dispersed in an aqueous phase containing 0.01% by weight of the exosomes derived from the proliferating stem cells at room temperature (15 C.), and then a reverse micelle emulsion (water/low-temperature process carbon dioxide) was prepared using supercritical carbon dioxide. Subsequently, the reaction was terminated, the supercritical carbon dioxide was vaporized under reduced pressure to remove the supercritical carbon dioxide phase, and a low-temperature process liposome suspension, in which the exosomes derived from the proliferating stem cells encapsulated, was obtained. Here, the temperature of the reaction process was 4 C. or below.

[0141] The cosmetic composition was prepared by the composition shown in Table 3 below using the liposome encapsulating the exosomes.

TABLE-US-00003 TABLE 3 Content Composition (% by weight) Stearic acid 2 Cetyl alcohol 2 Lanolin alcohol 2 Liquid paraffin 7 Cyclomethicone 5 Polyoxyethylene monooleic acid ester 2 Hexanediol 2 Glycerin 3 Triethylamine 5 Carbomer 0.2 Liposome encapsulating the exosomes 0.01 according to Example 2-1 of the present invention Purified water remainder

Formulation Example 1

Preparation of Skin Softening Cosmetic Water (Skin Lotion)

[0142] The skin softening cosmetic water (skin lotion) was prepared by the composition shown in Table 4 below using the liposome encapsulating the exosomes derived from the proliferating stem cells obtained by the method of Example 2-7.

TABLE-US-00004 TABLE 4 Content Composition (% by weight) Liposome encapsulating the exosomes 0.01 according to Example 2-1 of the present invention Ethanol 10 Glycerin 3 Butylene glycol 3 Sodium hyaluronate 0.1 Triethanolamine 0.1 Antioxidants 0.1 Preservatives, flavoring, coloring 0.1 Purified water remainder

Formulation Example 2

Preparation of Nutritive Cosmetic Water (Milk Lotion)

[0143] The nutritive cosmetic water (milky lotion) was prepared by the composition shown in Table 5 below using the liposome encapsulating the exosomes derived from the proliferating stem cells obtained by the method of Example 2-7.

TABLE-US-00005 TABLE 5 Content Composition (% by weight) Liposome encapsulating the exosomes 0.01 according to Example 2-1 of the present invention Glycerin 5 Mineral oil 4 Beeswax 4 Polysorbate-60 1.5 Carboxyvinyl polymer 0.1 Butylene glycol 3 Squalane 5 Triethanolamine 0.15 Preservatives, flavoring, coloring 0.1 Purified water remainder