BIOLOGICAL PRODUCT FOR CLEARING OF WATER, INDUSTRIAL WASTEWATER AND SOIL FROM CHEMICALS, WHICH ARE RESISTANT TO DEGRADATION AND METHOD FOR USING THE SAME

Abstract

A biological product for the purification of water, soil, industrial wastewater from chemicals that are resistant to degradation, such as pesticides and oil, contains a composition of strains of bacteria Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999, and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2);(1-2):1. The biological product can further contain sorbent, organic and mineral supplements, and stimulating agents. The biological product can be used for the decomposition of degradation-resistant pesticides and oil and has plant growth stimulating and fungicidal activity.

Claims

1. A method for the purification of ground, soil, water or industrial wastewater from stable to degradation pesticides, phenol or oil, that comprises introducing a product into contaminated soil, ground, water or wastewater, wherein the product comprises at least one selected from the group consisting of a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999 and Rhodococcus erythropolis VKPM Ac-1882, a composition containing strains Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B 10999, and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2):(1-2):1, and a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999 and Rhodococcus erythropolis VKPM Ac-1882 comprising a sorbent or being immobilized on the sorbent.

2. The method according to claim 1, wherein the stable to degradation pesticides are selected from the group consisting of chlorophenoxy acetic acids, 2,4-dichlorophenol, phenol, imidacloprid, dichloralurea, and tetramethylthiuram disulfide (TMTD).

3. A method for stimulating growth activity of plants and/or as fungicidal preparation, that comprises introducing a product in a foam of dry composition into a ground during ploughing, tillage, or seeding; or in the form of an aqueous solution into a ground by irrigation, wherein the product comprises at least one selected from the group consisting of a composition containing strains of Pseudomonas putida VKPM B-10997,Bacillus subtilis VKPM B-10999 and Rhodococcus erythropolis VKPM Ac-1882, a composition containing strains Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999, and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2):(1-2):1, and a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999 and Rhodococcus erythrapolis VKPM Ac-1882 comprising a sorbent or being immobilized on the sorbent.

4. The method according to claim 3, wherein the dry composition of the product is introduced into the ground in an amount of 10 kg per 1 ha of the ground.

5. A method for stimulating growth activity of plants and/or as fungicidal preparation, that comprises introducing seeds treated with a suspension of a product into a soil wherein the product comprises at least one selected from the group consisting of a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999 and Rhodococcus erythropolis VKPM Ac-1882, a composition containing strains Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999, and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2):(1-2):1, and a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999 and Rhodococcus erythropolis VKPM Ac-1882 comprising a sorbent or being immobilized on the sorbent.

6. The method according to claim 1, wherein the product comprises a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999 and Rhodococcus erythropolis VKPM Ac-1882.

7. The method according to claim 1, wherein the product comprises a composition containing strains Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999, and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2):(1-2):1.

8. The method according to claim 1, wherein the product comprises a composition containing strains of Pseudomonas putida VKPM B-10997,Bacillus subtilis VKPM B 10999 and Rhodococcus erythropolis VKPM Ac-1882 comprising a sorbent or being immobilized on the sorbent.

9. The method according to claim 8, wherein the composition further comprises mineral, organic and/or stimulating additives.

10. The method according to claim 2, wherein chlorophenoxy acetic acids comprise at least one selected from the group consisting of 2,4-dichlorophenoxyacetic acid (2,4-D), trichlorophenoxyacetic acid (2,4,5-T), chlorophenoxyacetic acid (CPAA), and phenoxyacetic acid (PAA).

11. The method according to claim 3, wherein the product comprises a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999 and Rhodococcus erythropolis VKPM Ac-1882.

12. The method according to claim 3, wherein the product comprises a composition containing strains Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999, and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2):(1-2):1.

13. The method according to claim 3, wherein the product comprises a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B 10999 and Rhodococcus erythropolis VKPM Ac-1882 comprising a sorbent or being immobilized on the sorbent.

14. The method according to claim 13, wherein the composition further comprises mineral, organic and/or stimulating additives.

15. The method according to claim 5, wherein the product comprises a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-I0999 and Rhodococcus erythropolis VKPM Ac-1882.

16. The method according to claim 5, wherein the product comprises a composition containing strains Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2):(1-2):1.

17. The method according to claim 5, wherein the product comprises a composition containing strains of Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999 and Rhodococcus erythropolis VKPM Ac 1882 comprising a sorbent or being immobilized on the sorbent.

18. The method according to claim 17, wherein the composition further comprises mineral, organic and/or stimulating additives.

19. The method according to claim 5, further comprises adding nutrients containing vermicompost.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0021] FIG. 1 is a plasmid profile of strains: 2-9 Pseudomonas putida VKPM B-10997, 10-17 Rhodococcus erythropolis VKPM Ac-1882.

[0022] The product is particularly effective when used for the decomposition of persistent pesticides, selected from the group of chlorofenoxyacetic acids such as 2,4-dichlorofenoxyacetic acid (2,4-D), trichlorofenoxyacetic acid (2,4,5-T), 2,4-dichlorophenoxy--propionic acid (Dichloroprop, 2,4-DR), 2-methyl-4-chlorophenoxy--propionic acid (Mekoprop, 2M-4HP, MSRR), 2,4,5-trichlorophenoxy--propionic acid (2,4,5-TP, Silvex), 2,4-dichlorophenoxy--oil (2,4-DV), methyl-[1-[(butylamino) carbonyl]-1H-benzimidazole-2-yl]carbamate and the product on its basis (benomyl, carbendazin, benazol); imidor, wherein the active substance is neonicotinoid imidacloprid; zontran, wherein the active substance is metribuzin, HCCH (hexachlorocyclohexane, hexachloran hexatox, dolmix, sineks) CPAA (chlorofenoxyacetic acid), PAA (fenoxyacetic acid), hexachlorophenol, 1,1-di(4-chlorophenyl)-2,2-dichloroethane (DDT) and 2,4-dichlorophenol and phenols. Particularly the product is effective when used for the decomposition of persistent pesticides from the group of chlorofenoxyacetic acids, such as2,4-dichlorofenoxyacetic acid (2,4D)), trichlorofenoxyacetic acid (2,4,5-T), chlorofenoxyacetic acid (CPAA), fenoxyacetic acid (PAA), as well as 2,4-dichlorophenol and phenol, and imidacloprid.

[0023] The product of the present invention unlike to the previously known products has additional growth stimulating effect on seed germination and growth of cultivated plants, and also has fungicidal activity.

[0024] The invention comprises also a method for use of the offered biological product PHENOX. Method consists of introducing of an effective amount of the product in contaminated gourd, soil or wastewater.

[0025] Preferably, the product is introduced in an amount of 10 kg of dry matter per 1 ha of land during ploughing at an ambient temperature, preferably at a temperature of 15-35 C. The preferred methods of administration are in the form of an aqueous solution by irrigation. The product can also be introduced to the ground by seeds treated with the product suspension with addition of biogenetic elements, such as vermicompost, or the introduction of a dry formulation of the product in the form of powder into the ground during ploughing or sowing.

[0026] The product can be introduced into the sod also by sowing seeds treated with the product suspension with the addition of additives, such as vermicompost. The product is diluted with water in the container by addition of vermicompost, nitrogen and phosphorus fertilizers.

[0027] The introduction of a dry formulation of the product in the form of powder in the ground during of ploughing or seeding is possible. The product PHENOX proposed according to the invention has a stimulating effect on plant growth, and strains of the productgood ability for adaptation to soil, which leads to an efficient detoxification of the environment. Strains of Pseudomonas putida and Rhodococcus erythropolis carry D-plasmids of the degradation and are able to transmit the property of destruction of pesticides and other harmful substances to indigenous bacterial populations in soil, water, wastewater, thereby increasing clearing from pollutants. The advantage of using this product is the decomposition of pesticides in soil for up to 86% and in liquid medium up to 99%.

[0028] The active basis of the offered product is the association of soil bacterial strains Pseudomonas putida, Bacillus subtilis, Rhodococcus erythropolis. All these cultures have polisubstratum specificity simultaneously to multiple xenobiotics: chlorofenoxyacetic acids, such as2,4-dichlorofenoxyacetic acid (2,4-D), trichlorofenoxyacetic acid (2,4,5-T), phenol, 2,4-dichlorophenol, CPAA and other specified in the examples. Their mixture ensures enhanced purification function of the environment from toxic compounds As sorbents kaolin and finely crushed peat are used also serving as an organic additive; as organo-mineral and growth stimulating supplements vermicompost, its extract and chitin containing substrates. Before use dry powder of the product is mixed well in the container (bucket, jar) with warm water (25-30 C.) until a homogeneous suspension, 10 g of the product is stirred into 10 liters of water. It is recommended to add azophoskaammonium nitrate based compound fertilizer (5 g/10 l) or compost (5 g/10 l), suspension periodically is stirred for aeration and microbial activation within 1-3 hours before use (watering the soil by watering-can (10-20 l/10 m.sup.2) or treatment of seeds 100-150 ml/100 g).

[0029] The biological product is obtained by a separate submerged cultivation, usually within 20-30 hours 3 strains of the said bacteria on the standard biotechnology equipment. Seeds are being obtained by growing strains in synthetic medium with the addition of pesticides and this material is being used for seeding large volume of fermenters.

[0030] Accumulated dry biomass of cells of the strains Pseudomonas putida, Bacillus subtilis, Rhodococcus erythropolis is mixed in a ratio of (1-2):(1-2):1. The total titre presents at least 510.sup.8 CFU/g of dry product after addition of carrier-sorbent, and other additives (the source concentrate of 10.sup.10-11 and at least 510.sup.8 CPU/mL liquid product). For the industrial production and packaging of the proposed product the standard biotechnological and packaging equipment and materials are used.

[0031] The offered bioproduct (PHENOX) eliminates penetration into plants, vegetables and berries plant protection chemicals, stimulates seed germination and plant growth, inhibits growth of fungal phytopathogens.

[0032] PHENOX is used for treating of contaminated by chemicals such as pesticides, phenol, possibly oil-water, industrial wastewater, soil and plant seeds. The strains contained in PHENOX are new.

[0033] The strain Bacillus subtilis B-10999 is isolated by the enrichment culture method from soil samples from the industrial zone of enterprises of chemical industry of the South Ural. The strain Bacillus subtilis was deposited in the Russian National Collection of industrial Microorganisms (VKPM) under the number B-10999. The strain of Bacillus subtilis is identified on the basis of nucleotide sequence analysis of 16S rRNA gene (SEQ. ID No. 1). The nucleotide sequence (length not less than 500 base pairs) of DNA fragment encoding the 16S rRNA gene, has similarity with the declared type of 99%.

[0034] Culture-Morphological Features.

[0035] The strain Bacillus subtilis has typical culture-morphological features for this kind. Rods, the size of which is 0.2-0.5 at 1.5-3 m, single, often in pairs. They form oval and round endospores. The flagella are placed over the entire cell's surface. When grown on meat-peptone broth the culture forms developed matte film, the media, however, remains clear. When shaking in liquid medium, the complete dispersion of biomass does not occur. Colonies are dry, finely wrinkled, with wavy edge. On slices of potato growing is abundant in the form of colonies of a cream colour smooth, strongly big size plicate. Gram-positive.

[0036] Physiological and Biochemical Features.

[0037] The strain Bacillus subtilis grows wed on glucose-peptone medium, LB agar and broth, glucose-mineral medium at a temperature of 28 C. It grows in media containing L-praline, DL-leucine, -ketoglutarate, DL--alanine, L-glutamine, D(+)-xylose, L-asparagine, chitin, DL-leucine, glucose, phenol and chlorophenoxy carbolic acids as the sole source of carbon. The strain Bacillus subtilis can be stored in a lyophilized state. Checking of the viability of the strain is carried out by planting of it on glucose-peptone agar, M9 and LBonce in 12 months. When it is stored on slants of these media at 5 C.replanting is carried out every 3 months. The strain Bacillus subtilis is a non-pathogenic and non-toxic micro-organism. Intravenously infected white mice do not show zoo pathogenic properties.

[0038] The strain Rhodococcus erythropolis Ac-1882 is isolated by the enrichment culture method from soil samples from the industrial zone of enterprises of chemical industry of the South Ural. The strain Rhodococcus erythropolis was deposited in the Russian National Collection of Industrial Microorganisms (VKPM) under the number Ac-1882. The strain Rhodococcus erythropolis is identified on the basis of nucleotide sequence analysis of 16S rRNA gene (SEQ. ID No. 2). The nucleotide sequence (length not less than 500 base pairs) of DNA fragment encoding the 16 S rRNA gene, has similarity with the declared type of 99%.

[0039] Culture-Morphological Features.

[0040] The strain Rhodococcus erythropolis has typical culture-morphological features for this kind. Cells rod-shaped, rarely branched, the diameter 0.6-0.8 m, the length3-8 m. Cells were moderately polymorphic, of arranged in a V-shape, have an explicit cycle of development coccus-rod-coccus: at the age of 24-36 hours rod-shaped cells begin to shorten and the share of coccoid and oval cells increase. Grown-up colonies are pigmented, orange coloured. The consistency of colonies is paste-like. Gram-positive.

[0041] Physiological and Biochemical Features.

[0042] The strain Rhodococcus erythropolis grows well on glucose-peptone medium, LB agar and broth, glucose-mineral medium at a temperature of 28 C. It grows in media containing L-proline, D-arabinose, DL--alanine, L-glutamine, L-asparagine, D(+)-xylose, L-asparagine, DL-serine, casein, glucose, phenol and chlorophenoxy carbolic acids as the sole source of carbon. The strain Rhodococcus erythropolis may be stored in a lyophilized state. Checking of the viability of the strain is carried out by planting of it on glucose-peptone agar, M9 and LBonce in 12 months. When it is stored on slants of these media at 5 C.replanting is carried every 3 months. The strain Rhodococcus erythropolis is a non-pathogenic and non-toxic micro-organism. Intravenously infected white mice do not show zoo pathogenic properties.

[0043] The strain Pseudomonas putida B-10997 is isolated by the enrichment culture method from soil samples from the industrial zone of enterprises of chemical industry of the South Ural. The strain Pseudomonas putida was deposited in the Russian National Collection of Industrial Microorganisms (VKPM) under the number B-10997. The strain Pseudomonas putida is identified on the basis of nucleotide sequence analysis of 16S rRNA gene (SEQ. ID No. 3). The nucleotide sequence (length not less than 500 base pairs) of DNA fragment encoding the 16S rRNA gene, has similarity with the declared type of 99%

[0044] Culture-Morphological Features.

[0045] The strain Pseudomonas putida has typical culture-morphological features for this kind. Motile rods with polar located flagella. The size of the cells is 1-4 on 1.3-5.4 m. Colonies on meat-peptone agar are grayish coloured, within the colonies and at their reverse side there is a reddish-brownish pigment. Spores do not occur. The consistency of the colonies is viscid. Gram-negative.

[0046] Physiological and Biochemical Features.

[0047] The strain Pseudomonas putida grows well on glucose-peptone medium, LB agar and broth, glucose-mineral medium at a temperature of 28 C. Grows in media containing DL-tyrosine, DL-tryptophan, D(+)-xylose, D()-mannitol, L-glutamic acid, DL-leucine, -ketoglutarate, DL--alanine, D-ribose, L(+)arabinose, L-glutamine, cellulose, L-asparagine, chitin, DL-serine, pectin, glucose, casein, and phenol and chlorophenoxy carbolic acids as sole source of carbon. The strain Pseudomonas putida can be stored in a lyophilized state. Checking of the viability of the Strain is carried out by planting of it on glucose-peptone agar, M9 and LBonce in 12 months. When it is stored on slants of these media at 5 C.replanting is carried out every 3 months. The strain Pseudomonas putida is a non-pathogenic and non-toxic micro-organism. Intravenously infected rabbits do not show zoo pathogenic properties.

EXAMPLES OF IMPLEMENTATION OF THE INVENTION

Example 1

[0048] Identification of plasmid degradation of pesticides in the strains. Destructive properties of bacteria can be determined by extrachromosomal elements [Don, Pemberton, 1985; Ghosaletal., 1985]. These elementsplasmids were found in the strains Pseudomonas putida VKPM B-0997 and Rhodococcus erythropolis VKPM Ac-1882. They were isolated by alkaline lysis method (Maniatis T., Fritsch, E., and Sambrook, J. Methods of genetic engineering. Molecular cloning: Trans. from English./Ed. AA Baev.M.: Mir, 1984. p. 480) from the cells of these strains and then plasmid products were fractionated in agarose gel under standard conditions (Maniatis et al., 1984)see FIG. 1 Plasmid profile of strains 2-9 Pseudomonas putida VKPM B-10997, 10-17 Rhodococcus erythropolis VKPM Ac-1882).

Example 2

[0049] Compatibility of strains contained in the product. The absence of an antagonistic activity between strains of the bacteria Bacillus subtilis VKPM B-10999, Rhodococcus erythropolis VKPM Ac-1882 and Pseudomonas putida VKPM B-10997 of the product was determined by the growth or strains in the contact lines in the media and by the method of agar blocks. On the LB agar medium the stroke of strain B. subtilis VKPM B-10999 was applied and perpendicular to it strokes of strains Rhodococcus erythropolis VKPM Ac-1882 and Pseudomonas putida VKPM B-10997 were applied. On the next cup on nutrient medium the stroke Rhodococcus erythropolis Ac-1882 was applied and perpendicular to it strains Bacillus subtilis B-10999 and Pseudomonas putida B-10997 were applied. On the third cup on medium the stroke of Pseudomonas putida B-10997 was applied and perpendicular to it the strains Bacillus subtilis B-10999 and Rhodococcus erythropolis Ac-1882 were applied. In the regions of contact lines inhibiting the growth of any of the strains was not observed. When the experiment was carried out using agar blocks, on the lawn of one of the strains on LB medium blocks of 3-days cultures of the other two strains were laid. No experiments showed inhibiting the growth of strains. This indicated the absence of antagonistic interactions between the strains and their possible effective joint application.

[0050] Examples of Compositions of the Biological Product PHENOX

Example 3

[0051] Bioproduct PHENOX, its forma dry powder. Dry biomass of cells (concentrate)Bacillus subtilis VKPM B-10999 no less than 510.sup.9 CFU/g, Pseudomonas putida VKPM B-10997 at least 510.sup.9 CFU/g and Rhodococcus erythropolis VKPM Ac-1882 at least 510.sup.8 CFU/g in the ratio (1-2):1:(1-2) (part of mass76.5-90%); without or with the introduction of sorbentfinely milled peat1-10% and/or stimulating additives, also containing mineral salts and trace elementsvermicompost0.1-1%; water, not more than 10.0%; the total titre of living cells for at least 10.sup.10 CFU/g.

Example 4

[0052] Bioproduct PHENOX, in the form of a dry powder. Dry biomass of cellsBacillus subtilis VKPM B-10999, Pseudomonas putida VKPM B-I0997 and Rhodococcus erythropolis Ac-1882 in the ratio 1:1:15-30%; sorbent-finely milled peat1-10% and/or stimulating supplement containing also mineral salts and trace elementsvermicompost0.1-1%; water, not more than 10.0%; sorbent and tiller kaolinup to 100%; the total titre of viable cells for at least 510.sup.8 CFU/g.

Example 5

[0053] Bioproduct PHENOX, in the form of a dry powder. Dry biomass of cellsBacillus subtilis VKPM B-10999, Rhodococcus erythropolis Ac-1882 and Pseudomonas putida VKPM B-10997 in the ratio 1:1:1 in total5-30; water, not more than 10.0%: kaolinup to 100%; the total titre of viable cells for at least 510.sup.8 CFU/g.

Example 6

[0054] Bioproduct PHENOX in liquid form: cells of bacteria Bacillus subtilis VKPM B-10999, Rhodococcus erythropolis Ac-1882 and Pseudomonas putida VKPM B-10997 in the ratio 1:1:1 (total titre10.sup.9 CFU/ml) in culture medium without additives or with addition of biologically active substances (extract of compost or vermicompost1-5 ml/l or finely milled compost or vermicompost 0.1% and/or chitin or chitosan or chitin containing raw materials0.05-0.4%), Possible to store at 0-8 C. for at least 3 months, at temperatures of 18-25 C. up to 15 days. When stored at temperatures of 18-25 C. it is allowable to decrease the number of viable cells to a titre of 10.sup.7 CFU/ml.

[0055] The method of use of the offered product is explained by the following examples:

Example 7

[0056] Use of the product PHENOX for treatment of water from imidacloprid. The product PHENOX in the form of a dry powder in the following composition: concentrate of the bacterial cells in ratio of 1:2:1 with a total titre 510.sup.10 CFU/g (the composition of the product according to Example 1) in the amount of 100 mg was introduced in 200 ml of tap water contaminated with a pesticide (insecticide) imidacloprid at concentrations of 1 mg/L and 2 mg/l of an active ingredient. In addition in the aquatic medium additives of mineral salts were added (g/l): Na.sub.2HPO.sub.4.7H.sub.2O-6.4; KH.sub.2PO.sub.4-1.5; NaCl 0.25 g/l; NH.sub.4Cl 0.5 g/l. For the control the samples of water contaminated with imidacloprid at concentrations of 1 mg/L and 2 mg/l with mineral salts without biological product were used. The samples were incubated at ambient temperature with constant stirring in a shaker (120 rpm). The experiment was repeated 3 times. The content of imidacloprid in water was determined by standard chromatographic methods at the 1st, 3rd, 5th, 7th and 10.sup.th day (Rudakov et al, 2004). The coefficient of variation of data was no higher than 5%.

TABLE-US-00001 TABLE 1 Dynamics of the content of imidacloprid in water when using the product PHENOX The content of imidacloprid, mg/l The variation 0 day 1.sup.st day 3.sup.rd day 7.sup.th day 10.sup.th day The control - 1.00 0.98 0.98 0.94 0.96 imidacloprid 1 kg/ml The control - 2.00 2.09 2.01 1.93 1.95 imidacloprid 2 kg/ml Biological product + 1.00 0.94 0.81 0.48 0.28 imidacloprid 1 kg/ml Biological product + 2.00 1.57 1.15 0.83 0.64 imidacloprid 2 kg/ml

[0057] The use of a biological product leads to a significant reduction of the content of imidacloprid in contaminated water. Reducing of the concentration of pesticide at the starting contamination of water by imidacloprid 1 mg/l at the 10.sup.th day was 0.28 (72% less). At contamination of water at a dose of 2 mg/l introduction of the biological product reduces the concentration of imidacloprid to 0.64 mg/l, (68% less). These data indicate that the biological product PHENOX allows purification of water from imidacloprid.

Example 8

[0058] The use of a biological product PHENOX for the purification of water from phenol. Bioproduct PHENOX in the liquid form of the following composition: bacterial cells at a ratio of 1:11 with a titre of 510.sup.9 CFU/g (a product composition according to Example 1, without addition of stimulating ingredients) in the amount of 0.01% of the volume was introduced into the water contaminated with phenol (the content of phenols is 100 mg/l). In the aquatic media additionally mineral nitrogen-phosphorus fertilizer in an amount of 0.5 g/l was introduced. For aeration and mixing air was being periodically supplied by a pump in the container with contaminated water. The temperature of water was maintained about 22-25 C. The determination of phenol in the water was conducted by a standard photometric method (Rudakov et al, 2004). The experiment was repeated 3 times. The coefficient of variation of data was no higher than 7%.

TABLE-US-00002 TABLE 2 Dynamics of the content of phenol after treatment of water with the product PHENOX The concentration of phenol, Incubation time Mg/l 0 day 100.0 1.sup.st day 73.0 3.sup.rd day 67.0

[0059] When using the product PHENOX the concentration of phenol in the water during the first day decreases by 27%, and till the 3.sup.rd dayby 33% (Table 2).

Example 9

[0060] Use of the product PHENOX for the purification of water from dichlorophenol. The experiment was carried out according to Example 8, but using the ratio of strains of 2:1:1 and water contaminated with dichlorophenol 2,4-DCP (the content of dichlorophenol was 100 mg/l).

TABLE-US-00003 TABLE 3 Dynamics of the content of dichlorophenol after treatment of water with the product PHENOX The concentration of Incubation time 2,4-DCP, mg/l 0 day 100.0 1.sup.st day 30.0 3.sup.rd day 21.9

[0061] When using the product PHENOX for the purification of water from dichlorophenol the concentration of 2,4-DCP during the 1st day of incubation decreases at 70% (Table 3).

Example 10

[0062] Use of the product PHENOX for the purification of water from 4-chlorophenoxy acetic acid. The experiment was carried out according to Example 8, but using the ratio of strains 1:2;1 and water contaminated with 4-4-chlorophenoxy acetic acid 4-CPAA (the content of 4-CPAA was 100 mg/l).

TABLE-US-00004 TABLE 4 Dynamics of the content of 4-chlorophenoxy acetic acid after treatment of water with the product PHENOX The concentration of Incubation time 4-CPAA, mg/l 0 days 100.0 1 day.sup. 96.3 3 days 95.8 5 days 94.1 7 days 92.5 9 days 90.0 11 days 68.7 13 days 63.9 15 days 62.0

[0063] The use of the product PHENOX for the purification of water from 4-chlorophenoxy acetic acid by the 9.sup.th day reduces the concentration of 4-CPAA in water for about 10%, and by the 15.sup.th day-for 38.0% (Table 4).

Example 11

[0064] Use of the product PHENOX for the purification of water from 2,4-dichlorofenoxyacetic acid. The experiment was carried out according to Example 8, but using water contaminated with 2,4-dichlorofenoxyacetic acid 2,4-D (the content of 2,4-D was 100 mg/l).

TABLE-US-00005 TABLE 5 Dynamics of the content of 2,4-dichlorofenoxyacetic acid after treatment of water with the product PHENOX The concentration of Incubation time 2,4-D, mg/l 0 days 100.0 1 day 95.3 3 days 89.4 5 days 81.5 7 days 74.3 9 days 70.0 11 days 68.6 13 days 66.9 15 days 65.0

[0065] When using the product PHENOX for the purification of water from 2,4-dichlorofenoxyacetic acid the content of 2,4-D till the 3rd day gradually falls for 11%, till the 9.sup.th day for 30% and the 15.sup.th day for 35%.

Example 12

[0066] Use of the product PHENOX for the purification of water from 2,4,5-trichlorofenoxyacetic acid. The experiment was carried out according to Example 8, but using water contaminated with 2,4,5-trichlorofenoxyacetic acid 2,4,5-T (the content of 2,4,5-T was 100 mg/l).

TABLE-US-00006 TABLE 6 Dynamics of the content of 2,4,5-trichlorofenoxyacetic acid after treatment of water with the product PHENOX The concentration of Incubation time 2,4,5-T, mg/l 0 days 100.0 1 day.sup. 96.3 3 days 85.0 5 days 64.8 8 days 55.0

[0067] When using the product PHENOX for the purification of water from 2,4,5-trichlorofenoxyacetic acid the concentration of 2,4,5-T to the 3.sup.rd day reduces for 15%, to the 8.sup.th day for 45% compared with the initial level (Table 6).

Example 13

[0068] Use of the product PHENOX for the purification of water from phenol. The experiment was carried out according to Example 12, but using the product PHENOX in the form of a dry powder (according to Example 3, with a titre of 510.sup.11 CFU/g and the introduction of vermicompost0.1%).

TABLE-US-00007 TABLE 7 Dynamics of the content of phenol after treatment of water with the product PHENOX The concentration of Incubation time phenol, mg/l 0 days 100.0 1 day.sup. 70.0 3 days 45.0

[0069] When using the product PHENOX in the form of a dry powder with catalytic additive for the purification of water from phenol its content reduces by the end of the 1.sup.st day for 30%, by the end of the 3.sup.rd day for 55% compared with the initial level (Table 7).

Example 14

[0070] Use of the product PHENOX for the purification of water from 2,4-Dichlorofenoxyacetic acid. Experiment was carried out according to Example 13, but using the product according to Example 3, without vermicompost with a titre of 10.sup.11 CFU/g and water contaminated with 2,4-dichlorofenoxyacetic acid, 2,4-D (the content of 2.4-D was 100 mg/l).

TABLE-US-00008 TABLE 8 Dynamics of the content of 2,4-dichlorofenoxyacetic acid after treatment of water with the product PHENOX The concentration of Incubation time 2,4-D, mg/l 0 days 100.0 2 days 81.3 4 days 64.2 6 days 61.8 8 days 60.5 10 days 59.0

[0071] When using the product PHENOX for the purification of water from 2,4-dichlorofenoxyacetic acid leads to a gradual decrease of the concentration of 2,4-Don the 10.sup.th day it drops to 59% from the starting value (Table 8).

Example 15

[0072] Use of the product PHENOX for the purification of water from dichlorophenol. The experiment was carried out according to Example 13, but using water contaminated with dichlorophenol 2,4-DCP (the content of dichlorophenol was 100 mg/l).

TABLE-US-00009 TABLE 9 Dynamics of the content of dichlorophenol in the treatment of water with the product PHENOX The concentration of Incubation time 2,4-DCP, mg/l 0 days 100.0 2 days 96.2 4 days 91.4 6 days 86.9 8 days 80.5 10 days 75.7 12 days 70.1 14 days 64.9 16 days 60.8 18 days 58.7 20 days 56.6 22 days 53.0

[0073] When using the product PHENOX for the purification of water from dichlorophenol the concentration of 2,4-DCP in 22 days drops to 53% from the starting value (Table 9).

Example 16

[0074] Use of the product PHENOX for the purification of water from 2,4,5-trichlorofenoxyacetic acid. The experiment was carried out according to Example 8, but using the product with stimulant additives and titres 10.sup.9 CFU/g and water contaminated with 2,4,5-trichlorofenoxyacetic acid 2,4,5-T (the content of 2,4,5-T was 100 mg/l).

TABLE-US-00010 TABLE 10 Dynamics of the content of 2,4,5-trichlorofenoxyacetic acid in the treatment of water with the product PHENOX The concentration of Incubation time 2,4,5-T, mg/l 0 days 100.0 2 days 70.0 4 days 35.2 6 days 33.8 8 days 33.1 10 days 32.6 12 days 31.0 14 days 31.9 16 days 38.7 18 days 35.8 20 days 28.6 22 days 30.1 24 days 31.4 26 days 18.3 28 days 16.0

[0075] The content of 2,4,5-T in water consistently reduces after treatment by PHENOXtill the 2.sup.nd day for 30%, till the 12.sup.th day for 69%, and till the 28.sup.th day for 84% (Table 10).

Example 17

[0076] Use of the bioproduct PHENOX for the purification of wastewater from phenols. Bioproduct PHENOX in the form of a dry powder of the following composition: dry biomass of cells Bacillus subtilis VKPM B-10999, Rhodococcus erythropolis Ac-1882 and Pseudomonas putida VKPM B-10997 in a ratio 1:1:1 in the amount10%, vermicompost1%, water not more than 10%, kaolinup to 100%, titre of alive cells210.sup.9 CFU/g (prepared according to Example 4). Before using a dry powder of the product was mixed well in the container (bucket, jar) in warm water (25-30 C.) until obtaining a homogeneous suspension. In the container having a working volume, equipped with a stirrer or a device for bubbling air, 1.0 kg of the biological product is introduced, 0.5 kg of azophoska and 200 l of warm technical water was added. To activate the microorganisms the suspension was stirred or perflated with air for 24 hours. Then a working suspension of the product was prepared and 10 kg of fertilizer (azophoska) and 1 kg of lime was added to the wastewater having volume of 10 m.sup.3 in the container. The container with wastewater was perflated by air daily. Wastewater pollution of Ufahimprom by phenolic compounds was 30.4 mg/liter. The efficiency of the treatment of wastewater from phenols by biological product PHENOX was evaluated without additives and with addition of mineral fertilizers in the wastewater (Table 11).

TABLE-US-00011 TABLE 11 Dynamics of the decontamination of wastewater from phenols using biological product PHENOX Period after the introduction of strain of a biological product PHENOX, into the wastewater, days The efficiency of The content the treatment of of phenol, mg/l wastewater from phenols, % Variation 0 2 5 7 0 2 5 7 Waste- 30.4 10.2 0.01 0.01 0 66.400 99.970 99.970 water Waste- 30.4 3.2 0.001 0.001 0 89.500 99.997 99.997 water with addition of mineral salts

[0077] The degree of purification of wastewater from phenols on the second day after the introduction of the biological product PHENOX reached 66.4%, and on the 5.sup.th day was for more than 99%. Effectiveness of the use of the biological product PHENOX for the treatment from phenols increases by the addition of mineral salts in the wastewater, already on the 2.sup.nd day the degree of purification of wastewater from phenol reached 89.5%, and on the 7.sup.th daywastewater was released from phenols by more than 99.997% (Table 11).

Example 18

[0078] Use of the biological product PHENOX for the treatment of industrial wastewaters from phenols. Treatment of industrial wastewaters was carried out according to the previous example, without addition of fertilizer, but using wastewater of LLC Novo-Ufimsky NPZ and JSC Dubitel.

TABLE-US-00012 TABLE 12 Dynamics of the decontamination of industrial wastewater from phenols by using the biological product PHENOX Period after the introduction of the biological product PHENOX in the wastewater, in days The content of The content phenol, mg/l of phenol, mg/l Variation 0 1 3 7 0 1 3 7 Wastewater of a 0.096 0.064 0.012 0.012 0 66.7 87.5 87.5 petrochemical company Wastewater of 0.750 0.155 0.155 0 79.4 79.4 tanning industry

[0079] The degree of purification of wastewater from phenols of the petrochemical enterprise on the first day after the introduction of biological product PHENOX was 66.7% and on the 3-7.sup.th day reaches 87.5%. The efficiency of use of the biological product PHENOX for the purification of wastewater of tanning industry from phenol during 3-7 days is 79.4% (Table 12).

Example 19

[0080] Use of the product PHENOX for the destruction of oil in an aqueous medium. The product in the form of dry powder (composition according to Example 3) with a titre of 110.sup.10 CFU/g in the amount of 100 mg, was introduced in 200 ml of water, contaminated with crude oil (at a concentration of 1%) with addition of salts (K.sup.2HPO.sub.40.05%, NH.sub.4Cl0.05%, CaCO.sub.30.001%). The oil-contaminated water after inoculation of product and mineral salts or nitrogen, phosphorus, potassium and lime was incubated for 10 days at ambient temperature under static conditions with daily stirring. Control was made by variation without introduction of the product. The experiment was repeated for 3 times. On the third day dark flakes in the volume of water and smaller than in the control variation stains of oil on the surface were observed. On the 5.sup.th day in the variation with the product a small amount of oil stains on the surface of water was observed, about for a half the film of oil was destroyed on the walls of the flask. By the 10.sup.th day of the experiment a small amount of oil on the strike of water and on the walls of the container was observed. Mass concentration of oil in the samples was measured on the 10.sup.th day in the concentratometer KH-2 (manufacturing company LLC PEP SIBEKOPRIBOR, Novosibirsk) by the spectrophotometric method in terms of selective absorption of petroleum products in the infrared area of spectrum at a wavelength of 3.42 micrometers. [The method of measurement of mass concentration of NP in the samples of drinking water, natural and wastewater by infrared spectrophotometry PND F 14.1:2:4, 168-2000, Novosibirsk, 1998, 17 p]. The coefficient of the variation of datano more than 5%. Use of the product reduces the amount of oil in water for 28% (from 2652 mg/l in control variation up to 1915 mg/ml in the variation with the biological product).

Example 20

[0081] Adaptation of bacterial strains of the product in the soil. Dynamics of the population of bacterial strains of the product in the soil was studied in model experiments. A suspension of cells in the amount of 310.sup.8 cells per 1 g of soil was placed in glass flask with 50 g of leached top soil. The experiment included 3 variations: without xenobiotic (the control) and 2,4-D at concentrations of 100 and 10 000 MPC. MPC for the 2,4-D in soils0.1 mg/kg. Soil moisture was 60% of full capacity. Periodically the strains of the product PHENOX were isolated on minimal salt agar medium M9 with the addition of 2,4-D as the sole source of carbon and energy. The number of colony forming units (CFU) of bacterial strains in the control soil increased within first 30 days, and then there was a decline of population density of these bacteria (Table 13). In polluted 2,4-D the density of the bacterial population was higher in the first 2.5 weeks after the introduction than in the controls, and then it gradually decreased. In the most polluted soil bacterial population of PHENOX sharply increases and 10-50 times exceeds the population density of these strains in the control. This rise of the number of bacteria PHENOX is due to using xenobiotic as a source of nutrition. Reducing the number of bacteria PHENOX in the soil in this variation occurs after 30 days. By day 52.sup.nd the density of the population of introduced strains in all variants was at a similar level, indicating a decrease of the concentration of 2,4-D in contaminated soils, as well as possible involvement in the degradation of pesticides of other microorganisms.

[0082] A good adaptation of the microorganisms to soils was revealed. Similar data were obtained in experiments with other types of soils-gray forest, podsol soil and xenobiotics as three concentrations of 100 MPC, 1000 MPC, 10 000 MPC. MPC for the 2,4-D in soils is 0.1 mg/kg. This shows the possibility of the use of the product of the present invention in these types of soils.

TABLE-US-00013 TABLE 13 Dynamics of the number of strains of the product PHENOX introduced to the contaminated by pesticide 2,4-D leached top soil Dynamic of the number of cell population CFU 10.sup.5/g of soil Soil with Soil with Soil with 2,4-D 100 2,4-D 1000 2,4-D 10000 Time Net soil MPC MPC MPC 0 days 0.00 0.00 0.00 0.00 9 days 0.37 4.33 4.33 0.66 19 days 13.40 50.50 4.30 700.00 30 days 70.00 55.40 9.00 340.50 52 days 1.70 3.00 2.70 0.98

Example 21

[0083] Use of a biological product PHENOX for the treatment of soil contaminated with pesticide 2,4,5-T (trichlorofenoxyacetic acid). Soil (100 g) of arable horizon was sieved through a sieve of 2 mm diameter and placed in glass flasks for 500 ml. 2,4,5-T was introduced in soil in the amount of 100 mg/g and mixed thoroughly. Bioproduct PHENOX with a titre of 10.sup.9 CFU/g. (according to Example 5) was inoculated into a bottle with the soil, moistened to 60% of capacity and also mixed. Flasks were closed with cotton plugs with parafilm and incubated at constant humidity and temperature of 25 C. in a thermostat. Determination of 2,4,5-T in soil was carried out by the standard method (The method of pesticides measurement in soil. M.E., Medicine, 1984,p. 256 p.). The experiment was repeated 3 times. The coefficient of variation of data was no higher than 8%.

TABLE-US-00014 TABLE 13* Dynamics of the decontamination of soil from 2,4,5-T using the biological product PHENOX Time after treatment, days Parameter 1 5 10 14 21 30 48 The content of 2,4,5-T 100 95.0 87.6 85.0 72.6 68.1 33.5 in soil, mg/g The degree of 0 5.0 12.4 15.0 27.4 31.9 66.5 purification comparing to control, %

[0084] The degree of soil decontamination from 2,4,5-T by using the biological product PHENOX in 14 days was 15%, in 21 days 27.4%, and in 30 days31.9% and reached 66.5% by the 48.sup.th day. The rate of decomposition of 2,4,5-T increases significantly after 30 days by incubation of soil with biological product PHENOX (table 13*).

Example 22

[0085] Use of the product PHENOX for the disintegration of pesticide (fungicide) TMTD in soil. The venue of the experiment: concrete ground, adjacent to the warehouse for storage of pesticides (The region of Altai, Biysk city, 4 Prigorodnaya Street). In summer time on an open concrete ground a suit plot area of 3 m.sup.2 (13 m) was arranged, delimited by wooden partitions with height of 20 cm at 3 sites 11 m. The bottom of the plots was spread with plastic film to prevent the spread of pollutants from the soil to concrete ground. Plots were filled with a layer of top soil with thickness of 15 cm. At a height of 60 cm from the ground plots were covered by non-woven covering material (spunbond). In the soil the pesticide (fungicide) tetramethylthiuram disulfide (TMTD) at the rate of 50 g/m.sup.2 was introduced, vermicompost at the amount 50 g/m.sup.2, mineral nitrogen and phosphorus fertilizer enriched with micronutrients100 g/m.sup.2 and organic fertilizers based on peat400 g/m.sup.2 and mixed thoroughly. Product PHENOX in the form of dry powder composition: bacterial cells10%, vermicompost1%, kaolin89% with a titre of 510.sup.9 CFU/g (according to example 4) of 300 g was dissolved in 15 liters of water (25-30 C.) and incubated 24 hours with periodic agitation to improve aeration and activate miero-organisms. The suspension of biological product was applied to a surface of the plot at the rate 5 litres/m.sup.2 by sprinkling with a watering can. The surface of the plot was further watered by 5 litres/m.sup.2 of soil. Soil contaminated with TMTD, after treatment with the product PHENOX was loosened to improve aeration and mulched by a thin layer of sawdust. If necessary, and in case of no rain the soil in the plot was occasionally loosened and moistened up to 60-70% of full capacity. To evaluate the effectiveness of soil decontamination from TMTD by the method of sample middling, samples were taken each 1.5 and 3 months. The quantitative content of TMTD in the samples was determined chromatographically. The coefficient of variation of data was not higher than 5%. 1.5 months after the treatment with the product PHENOX the content of TMTD in soil reduced by 53%, in 3 monthsby 75%.

Example 23

[0086] Use of the product PHENOX for the decomposition of the pesticide (herbicide) dichloralurea in soil. The experiment was conducted similar to the Example 22. The pesticide (herbicide) dichloralurea was introduced into the soil at the rate of 50 g/m.sup.2 1.5 months alter the treatment with the product PHENOX the content of tetramethylthiuram disulfide TMTD in soil reduced by 59%, in 3 monthsby 78%.

Example 24

[0087] Use of the product PHENOX for the purification the soil from chlorinated aromatic compounds. Venue of the experiment: Rostovskaya Oblast, Salski region. Characteristics of the plot: land for agricultural purposes, contaminated with chlorinated aromatic compounds. In summer time in an open air a plot area of 530 m.sup.2 (size of 66 m8 m) was marked, delimited in areas of 170 m.sup.2 by protective zones of 0.5 m. The experiment was repeated 3 times, in contaminated soil vermicompost in the amount of 100 g/m.sup.2, mineral nitrogen and phosphorus fertilizer at the rate of 10 g/m.sup.2 were introduced. The product PHENOX in the form of dry powder composition: bacterial cells10%, vermicompost1%, kaolin89% with a titre of 510.sup.9 CFU/g (according to Example 4) in an amount of 10 g/m.sup.2 to obtain a working solution was dissolved in a container and mixed well in warm water (25-30 C.) until a homogeneous suspension. Working suspension of the product PHENOX was applied to the surface of the soil at the rate of 10 l/m.sup.2. The application was carried out by sprinkling with any provided for this purpose machines and devices. The treatment was performed at an average daily soil temperatures at least +5 C. and not above +30 C. The introduced biologic product and fertilizers were worked in the upper layer of the contaminated soil by plowing, digging, cultivation or other methods of tillage. The soil periodically was loosened to improve aeration and humidified up to 60-70% of full capacity (if necessary, in case of the absence of rain). After two weeks the soil at the site was plowed and humidified. To evaluate the effectiveness of soil decontamination from chlorinated aromatic compounds by the method of sample middling, samples were taken every 1.5 and 3 months. The quantitative content of aromatic compounds in the samples was determined by the standard method (Rudakov et al, 2004). The coefficient of variation of data was not higher than 9%. 1.5 months alter the treatment with the product PHENOX the content of aromatic compounds in soil reduced by 35%, in 3 monthsby 68%.

Example 25

[0088] Assessment of fungicidal activity of the product (in the ratio 1:1:1). The fungicidal activity of bacterial strains B. subtilis VKPM B-10999, Rhodococcus erythropolis VKPM Ac-1582 and Pseudomonas putida VKPM B-10997 of the product was determined by the standard method of agar blocks (Egorov, 1976). Petri dish with a solid nutrient medium was sown with phytopathogenic fungus lawn culture. Strains of fungi was used, that caused destruction of the roots, wilting, mottling, root rotFusarium oxysporum, Alternaria tennuisima, Alternaria alternata, and gray mold on leaves and fruits of plantsBotrytis cinerea, rotting of agricultural productsGeotrichum candidum. Each culture was sown into 2 cups of Czapek's medium and on its surface the blocks of bacterial strains were placed, cut out of 5-days lawn of bacteria on standard LB-agar, 4 blocks of the bacterial strain sown by phytopathogenic fungus on each medium were placed. Cups were incubated for 2 days at 5 C. (for the diffusion of antibiotic substances in the agar), and thenat 25 C. and the existence of zones of growth of the inhibition of fungi was established.

TABLE-US-00015 TABLE 14 Fungicidal activity of bacterial strains of the product The size of areas of inhibition of the fungus on 3.sup.rd day, mm The bacterial Fusarium Alternaria Alternaria Botrytis Geotrichum strain oxysporum alternata tennuisima cinerea candidum Bacillus subtilis 1 3 * 1 Rhodococcus 1 2 2 2 erythropolis Pseudomonas 1 2 2 3 2 putida * There is no inhibition area

[0089] Fungicidal activity against all tested in the experiment of phytopathogenic fungi is established for the strain Pseudomonas putida B-10997, and for the strains of B. subtilis B-10999, Rhodococcus erythropolis Ac-1882to fungus causing predominantly root infections and decay (Table 14). That is the bacterial product has fungicidal activity to pathogens that determines various fungal diseases of plants.

[0090] The Plant Growth Stimulating Activity of the Product

Example 26

[0091] Use of the product to stimulate plant growth of radish. The product was in the form of dry powder of the following composition: bacterial cells10% (dry biomass of cells Bacillus subtilis VKPM B-10999, Pseudomonas putida VKPM B-10997 and Rhodococcus erythropolis Ac-1882 in the ratio 1:1:1) kaolin89% with a titre of 510.sup.9 CFU/g was dissolved in 10 ml of distilled water (dilution 1:10) and then sequential dilutions of 1:100 and 1:1000 were prepared. Radish seeds of the variety of Rose-red with a white tip were spread on filter paper in Petri dishes for 25 seeds in each Petri dish. The experiment was repeated 4 times. Seeds were treated with the product suspension in an amount of 2 ml for a Petri dish by using an automatic dispenser. Control variations were the seeds germinated in distilled water and seeds treated with 0.05% solution of indole-butyric acid (IBA)the stimulatator of the growth of plants of auxin type. Petri dishes with treated seeds were incubated under natural light and at ambient temperature in a humid chamber. The length of roots, the length of shoots and the dry weight of plants was determined at the 5.sup.th day. The coefficient of variation of data was no higher than 5%.

TABLE-US-00016 TABLE 15 Growth of radish plants when using the biological product PHENOX Parameter Length of Length of Dry weight the root, the seedling, (air-dry The variation of treatment mm mm weight) g The control (water) 69 30 0.27 The control (IBA) 70 37 0.35 The product dilution 1:10 61 41 0.34 The product dilution 1:100 81 42 0.37 The product dilution 1:1000 58 38 0.32

[0092] Suspension of the product stimulates the growth of the red radish and accumulation of biomass. The maximum effect was observed from the aqueous suspension of the product of 1:100. The product at a dilution of 1.100 increased the length of the 5 days radish roots from 69 to 81 mm (17%), length of seedling from 30 to 42 mm (40%) and dry weight of plants by 37%, from 0.27 to 0.37 g (Table 16).

Example 26

[0093] Use of the product PHENOX to stimulate plant growth of cabbage. The experiment was conducted similarly to the Example 25 (the content of the product: dry biomass of cellsBacillus subtilis VKPM B-10999, Pseudomonas putida VKPM B-10997 and Rhodococcus erythropolis Ac-1882 in the ratio 1:1:110%; sorbent-finely milled peat89%, vermicompost1%). Seeds of cabbage variety Slava were treated by the dilutions of the product. The length of roots and shoots of plants were determined after 5 days.

TABLE-US-00017 TABLE 16 Growth parameters of cabbage variety Slava when using the product Parameter Length of Length of The variation of treatment the root, mm the seedling, mm The control (water) 46 31 The control (IBA) 52 36 The product dilution 1:10 64 35 The product dilution 1:100 69 37 The product dilution 1:1000 63 37

[0094] Aqueous suspension of the product (at all tested dilutions) increases the length of roots up to 50%from 46 to 63-69 mm and length of shoots by 19%from 31 to 37 mm at a dilution of 1:100 and 1:1000 (Table 16). The results indicate a strong stimulating effect of the product on the growth of roots of cabbage variety Slava.

Example 27

[0095] Use of the product to stimulate the clover seed germination and plant growth. The experiment of conducted as in the Example 26. The red clover seeds were treated with the dilutions of the product. After 5 days the length of the root and the length of the seedling, germination and dry weight of plants were determined.

TABLE-US-00018 TABLE 17 Growth parameters of the red clover when using the biological product PHENOX Parameter Length of Length of Dry The variation of the root, the seed- Germi- weight, treatment mm ling, mm nation, % g The control (water) 18 30 88 0.07 The control (IBA) 23 33 91 0.09 The product dilution 1:10 34 40 84 0.12 The product dilution 1:100 24 33 100 0.15 The product dilution 1:1000 25 36 96 0.12

[0096] The product at a dilution of 1:10 increases the linear dimensions of the plant-root length by 89% (from 18 to 34 mm) and length of the seedling by 33% (from 30 to 40 mm). The product at a dilution of 1:100 enables improvement of germination by 12%from 88 to 100% and the increase of dry weight by 114%from 0.07 to 0.15 g (Table 17). Thus, the product has a strong stimulatory effect on root growth and increase of plant mass of clover.

Example 27

[0097] Use of the product to stimulate the cucumber plant growth. Experiment was conducted as in the Example 26. The seeds of cucumber variety Russkaya Rubashka F1 were treated by the dilutions of the product. The length of root and seedling, germination and plant dry weight was determined after 6 days.

TABLE-US-00019 TABLE 18 Growth parameters of cucumber plants when using the biological product PHENOX Parameter Length of Length of Dry The variation of the root, the seed- Germi- weight, treatment mm ling, mm nation, % g The control (water) 46 33 84 0.46 The control (IBA) 59 38 92 0.55 The product dilution 1:10 74 41 96 0.72 The product dilution 1:100 71 41 100 0.70 The product dilution 1:1000 64 36 92 0.63

[0098] Aqueous suspensions of the product have a stimulating effect on the growth of cucumber variety Russkaya Rubashka F1. For example, in an aqueous dilution of 1:10 product increases the length of the root at 61%from 46 to 74 mm, the length of the seedling at 24%from 33 to 41 mm, the germination at 16%from 84 to 100% and dry weight of plants by 37% from 0.46 to 0.63 g (Table 18). The greatest effect of the product is on root growth of cucumber.

Example 27

[0099] Use of the product to stimulate the beet seed germination. The experiment was conducted similarly to the Example 26. Beet seed variety Detroit was treated by the dilutions of the product. Seed germination was determined on the 7.sup.th day.

TABLE-US-00020 TABLE 19 Germination parameters of beet seed when using the biological product PHENOX Parameter The variation of treatment Germination, % The control (water) 92 The control (IBA) 95 The product dilution 1:10 96 The product dilution 1:100 99 The product dilution 1:1000 96

[0100] The product at a dilution of 1:100 increases germination of beet seeds by 7%from 92 to 99% (Table 19).

Example 28

[0101] Use of the product to stimulate the growth of onions plants. The experiment was conducted similarly to the Example 27. Onion seeds of variety Karmen were treated with the preparation at dilutions of 1:100 and 1:1000. The length of root and seedling, germination and plant dry weight is determined after 10 days.

TABLE-US-00021 TABLE 20 Growth parameters of onion plants when using the biological product PHENOX Parameter Length of Length of Dry The variation of the root, the seedling, weight, treatment mm mm g The control (water) 30 57 0.19 The control (IBA) 33 61 0.21 The product dilution 1:100 34 69 0.28 The product dilution 1:1000 37 62 0.25

[0102] The product at a dilution of 1:1000 increases the length of the root by 23% from 30 to 37 mm, the length of the seedling by 9% from 57 to 62 mm and the increase of plant dry weight by 32%, from 0.19 to 0.25 g (Table 20).

Example 29

[0103] Use of the product to stimulate the carrot plant growth. The experiment was conducted similarly to the Example 28. Carrot seeds of variety Krasniy Velikan were treated with the dilutions of the product. The dry weight of plants was determined after 10 days.

TABLE-US-00022 TABLE 21 Growth parameters of carrot plants when using the biological product PHENOX Parameter The variation of treatment dry weight, g The control (water) 0.03 The control (IBA) 0.05 PHENOX + BL-5 at the product dilution 1:10 0.04 PHENOX + BL-5 at the product dilution 1:100 0.04 PHENOX + BL-5 at the product dilution 1:1000 0.05

[0104] The product at the dilution of 1:1000 increased the dry weight of seedlings of carrots by 67%from 0.03 to 0.05 g (Table 21).

[0105] The presence of pesticides in soil, water and industrial wastewater was tested using the gas-chromatic method for the determining of pesticides described by Rudakov O. B. and others (Sputnik khromotografista.Voronezh: Vodoley, 2004.p. 528).

SOURCES OF INFORMATION

[0106] 1. Feodorov A. Yu., Volchenko E. V., Korzhenevich V. I., Singircev I. N., Krestyaninov V. Yu. Polysubstratnyj shtamm-destruktor komponentov stochnih vod proizvodstva fenola.//Prikladnaya biohimiya l mikrohiologiya.1993.1.29, N5.p. 716-722.

[0107] 2. Feodorov A. Yu., lgriatov O. V., Korzhenevich V. I., Shub G. M. Effect of immobilization in agar gel on the microbial degradation of xenobiotics.//6th European Congr. Biotechnology (ECB6), Firenze, Italy, Jun. 13-17, 1993.Firenze, 1993.Abstrs. Books, V.2.TU212.

[0108] 3. Korzhenevich V. I., Volchenko E. V. Singircev I. N., Feodorov A. Yu., Shoob G. M. Microbial treatment of phenolic wastes.In: Environmental Biotechnol,: Principles and Applications.M. Moo-Young et al, (eds.), Kluwer Academic Publishers, 1995.P. 498-503.

[0109] 4. Singircev I. N., Volchenko E. V., Korzhenevich V. I., Gumenyuk A. P., Feodorov A. Yu. Microbal degradation of components of phenolic waste waters.//Prikladnaya biohimiya l mikrobiologiya.2000.1.36, N2.p. 178-188.

[0110] 5. Authors' certificate No. SU 1597346; C02F3/34, C12N1/20: Strain Pseudomonas psendoalcaligenes used for treatment of wastes from aromatic compounds/Mironov A. D., Korzhenevich V. I., Barkovskiy A. L.OG No. 37, 1990.

[0111] 6. Mironov A. D., Krestyaninov Korzhenevich V. Yu., Korzhenevich V. I., Yevtushenko I. Ya., Barkovskiy A. L. Destrukciya 2-nitrobenzoynoy kisloty i drugih aromaticheskih sejedineniy shtammom Pseudomonas pseudoalcaligenes.//Prikladnaya biohimiya l mikrobiologiya.1991,1.27, N4,p. 571-576.

[0112] 7. Giardina M. C., Giardi M. T., Filacchione G. Atrazine metabolism by Nocardia: elucidation of the initial pathway and synthesis of potential metabolites.//Agric. Biol. Chem.1982.Vol. 46.P. 1439-1445/

[0113] 8. Blaszak M., Pelech R., Graczyk P. Screening of Microorganisms for Biodegradation of Simazine Pollution (Obsolete Pesticide Azotop 50 WP)//Water Air Soil Pollut.2011. Vol. 220. P. 373-385.

[0114] 9. Sajjaphan K. Heepnigoen P. Sadowsky M J. Boonkerd N. J Arthrobacter sp. strain KU001 isolated from a Thai soil degrades atrazine in the presence of inorganic nitrogen sources//Microbiol. Biotechnol.2010. Vol. 20.P. 602-608.