Medicinal agent exhibiting antiprotozoal activity to Trichomonas vaginalis in an in-vitro model system

Abstract

The invention belongs in the field of medicine and can be used in the treatment of chronic and asymptomatic trichomonas infections of the urogenital tract. The invention is consisted in development of new therapeutic substance of plant origin with minimal side effects for treatment of various forms of trichomonad infection. The new therapeutic substance, of plant origin, is an olive coloured paste. It is composed of a conifer green needle complex (CGNC) and has a coniferous smell and antiprotozoal activity against Trichomonas vaginalis (when tested in an in vitro model). At a concentration of 100 mg/ml CGNC has trichomonadocitic and trichomonadostatic properties. At 300 mg/ml it suppresses the growth of T. vaginalis and at 500 mg/ml it kills this organism.

Claims

1. A method of suppressing the growth of Trichomonas vaginalis in a patient infected therewith, comprising administering to the patient an effective amount of a composition comprising conifer green needle complex (CGNC).

2. The method according to claim 1, wherein the concentration of CGNC in said composition is at least 100 mg/ml.

3. The method according to claim 2 wherein said composition has trichomonadocitic or trichomonadostatic effects.

4. The method according to claim 1 wherein the concentration of CGNC in said composition is at least 300 mg/ml.

5. The method according to claim 1 wherein the concentration of CGNC in said composition is at least 500 mg/ml.

6. The method according to claim 1, wherein the patient is suffering from acute or chronic Trichomonas infection.

7. The method according to claim 6 wherein said infection is an infection of the urogenital tract.

8. The method according to claim 1, wherein the patient is suffering from asymptomatic Trichomonas infection.

Description

EXAMPLES OF THE PREFERRED EXECUTION OF INVENTION

(1) Below are Examples of Emulsion Based on the Therapeutic Substance

Example 1

(2) CGNC10.0 g

(3) Double distilled watermade up to 100 g.

Example 2

(4) CGNC30.0 g

(5) Double distilled watermade up to 100 g.

Example 3

(6) CGNC50.0 g

(7) Double distilled watermade up to 100 g.

(8) To prepare an emulsion (Examples 1-3), a mixing apparatus is loaded with calculated amount of CGNC, the paste is heated to 50 C., and calculated amount of double distilled water is added. Heating is stopped when the emulsion becomes homogeneous and the mixture is packed in glass bottles.

INDUSTRIAL APPLICATIONS

(9) The results obtained from the study showed the potential use of CGNC as treatment for acute, chronic and asymptomatic trichomonas infections. The use of CGNC as antiprotozoal therapy allows for the acceleration of the treatment process, inhibition of T. vaginalis and associated pathogenic microflora in urogenital tract infections such as those found in dysbacteriosis.