TRADITIONAL CHINESE MEDICINE COMPOSITION FOR EYEBROW ENHANCEMENT, EYEBROW GROWTH POWDER, PREPARATION METHOD AND USE THEREOF

Abstract

The disclosure provides a traditional Chinese medicine composition for eyebrow enhancement, an eyebrow growth powder, a preparation method and use thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition for eyebrow enhancement comprises the following components in parts by weight: 2-5 parts of chuanxiong rhizome, 5-8 parts of mulberry, 3-5 parts of sesame, 10-20 parts of Eclipta Herba, 10-20 parts of Platycladi cacumen, 3-5 parts of Rehmanniae Radix Preparata, 3-5 parts of Gardeniae Fructus and 3-5 parts of Ligustri Lucidi Fructus.

Claims

1. A traditional Chinese medicine composition for eyebrow enhancement, wherein comprising the following components in parts by weight: 2-5 parts of chuanxiong rhizome, 5-8 parts of mulberry, 3-5 parts of sesame, 10-20 parts of Eclipta Herba, 10-20 parts of Platycladi cacumen, 3-5 parts of Rehmanniae Radix Preparata, 3-5 parts of Gardeniae Fructus and 3-5 parts of Ligustri Lucidi Fructus.

2. An eyebrow growth powder, wherein comprising the following raw materials in parts by weight: 2-5 parts of chuanxiong rhizome, 5-8 parts of mulberry, 3-5 parts of sesame, 10-20 parts of Eclipta Herba, 10-20 parts of Platycladi cacumen, 3-5 parts of Rehmanniae Radix Preparata, 3-5 parts of Gardeniae Fructus, 3-5 parts of Ligustri Lucidi Fructus, 35 parts of glycerol tristearate, 60 parts of soybean lecithin, 45 parts of poloxamer 188, and 5 parts of water.

3. A method for preparing the eyebrow growth powder according to claim 2, wherein comprising: 1) pulverizing and mixing the chuanxiong rhizome, mulberry, sesame, Eclipta Herba, Platycladi cacumen, Rehmanniae Radix Preparata, Gardeniae Fructus and Ligustri Lucidi Fructus to obtain a compound; 2) mixing the compound obtained in step 1) with the glycerol tristearate to obtain an oil phase; 3) mixing the soybean lecithin, poloxamer 188 and water to obtain a water phase; 4) mixing the oil phase obtained in step 2) with the water phase obtained in step 3) to obtain a mixed phase, and sequentially carrying out high-speed shearing, ultrasonic treatment and high-pressure homogenization on the mixed phase to obtain a mixture; 5) freeze-drying the mixture obtained in step 4) to obtain the eyebrow growth powder.

4. The method according to claim 3, wherein a particle size of the compound in step 1) is less than 100 meshes.

5. The method according to claim 3, wherein a mixing temperature in step 2) is 80 C., and a mixing is carried out in a water bath.

6. The method according to claim 3, wherein a mixing temperature in step 3) is 100 C., and a mixing is carried out in a water bath.

7. The method according to claim 3, wherein the conditions of high-speed shearing in step 4) comprise: a rotating speed is 1000 rpm, and a time is 1-2 min; the conditions of ultrasonic treatment comprise: a time is 2 min, a power is 1000 w, and a frequency is 50 kHz; the conditions of high pressure homogenization comprise: a time is 4-6 min and a pressure is 50000 psi.

8. The method according to claim 3, wherein the conditions of freeze-drying in step 5) comprise: pre-freezing the mixture at 10 C. for 4-6 h, and then freezing at 45 C. and 1 kPa for 24-36 h.

9. Use of the traditional Chinese medicine composition according to claim 1 or the eyebrow growth powder in preparing a medicine for preventing eyebrow shedding.

10. Use of the traditional Chinese medicine composition according to claim 1 or the eyebrow growth powder in preparing an eyebrow growth medicine.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly explain the technical scheme in the embodiment of the present disclosure or in the prior art, the drawings needed in the embodiment will be briefly introduced below.

[0040] FIG. 1 shows the compatibility principle of the traditional Chinese medicine composition;

[0041] FIG. 2 is an experimental grouping diagram;

[0042] FIG. 3 is a comparison chart of the first day of each group's medication in example 1 of the present disclosure;

[0043] FIG. 4 is a comparison chart of the eighth day of each group's medication in example 1 of the present disclosure;

[0044] FIG. 5 is a comparison chart of the 10th day of each group's medication in example 1 of the present disclosure;

[0045] FIG. 6 is a comparison chart of the 12th day of each group's medication in example 1 of the present disclosure;

[0046] FIG. 7 is a comparison chart of the 15th day of each group's medication in example 1 of the present disclosure.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0047] The present disclosure provides a traditional Chinese medicine composition for eyebrow enhancement, which comprises the following components in parts by weight: 2-5 parts of chuanxiong rhizome, 5-8 parts of mulberry, 3-5 parts of sesame, 10-20 parts of Eclipta Herba, 10-20 parts of Platycladi cacumen, 3-5 parts of Rehmanniae Radix Preparata, 3-5 parts of Gardeniae Fructus and 3-5 parts of Ligustri Lucidi Fructus.

[0048] The present disclosure also provides an eyebrow growth powder, wherein comprising the following raw materials in parts by weight: 2-5 parts of chuanxiong rhizome, 5-8 parts of mulberry, 3-5 parts of sesame, 10-20 parts of Eclipta Herba, 10-20 parts of Platycladi cacumen, 3-5 parts of Rehmanniae Radix Preparata, 3-5 parts of Gardeniae Fructus, 3-5 parts of Ligustri Lucidi Fructus, 35 parts of glycerol tristearate, 60 parts of soybean lecithin, 45 parts of poloxamer 188, and 5 parts of water.

[0049] The present disclosure also provides a method for preparing the eyebrow growth powder according to the above technical scheme, wherein comprising: [0050] 1) pulverizing and mixing the chuanxiong rhizome, mulberry, sesame, Eclipta Herba, Platycladi cacumen, Rehmanniae Radix Preparata, Gardeniae Fructus and Ligustri Lucidi Fructus to obtain a compound; [0051] 2) mixing the compound obtained in step 1) with the glycerol tristearate to obtain an oil phase; [0052] 3) mixing the soybean lecithin, poloxamer 188 and water to obtain a water phase; [0053] 4) mixing the oil phase obtained in step 2) with the water phase obtained in step 3) to obtain a mixed phase, and sequentially carrying out high-speed shearing, ultrasonic treatment and high-pressure homogenization on the mixed phase to obtain a mixture; [0054] 5) freeze-drying the mixture obtained in step 4) to obtain the eyebrow growth powder.

[0055] In the disclosure, chuanxiong rhizome, mulberry, sesame, Eclipta Herba, Platycladi cacumen, Rehmanniae Radix Preparata, Gardeniae Fructus and Ligustri Lucidi Fructus are crushed and mixed to obtain a compound. In the present disclosure, the traditional Chinese medicine is preferably washed, drained and dried, and then subjected to superfine grinding, and the conditions for superfine grinding are not particularly limited in the present disclosure, and those skilled in the art can follow the routine. In the present disclosure, the particle size of the compound is preferably less than 100 meshes.

[0056] In the disclosure, the obtained compound is mixed with glycerol tristearate to obtain an oil phase. In the present disclosure, the mixing temperature is preferably 80 C., and the mixing is carried out in a water bath.

[0057] In the disclosure, the soybean lecithin, poloxamer 188 and water are mixed to obtain a water phase. In the present disclosure, the mixing temperature is preferably 100 C., and the mixing is carried out in a water bath.

[0058] In the disclosure, the obtained oil phase is mixed with the obtained water phase to obtain a mixed phase, and the mixed phase is sequentially subjected to high-speed shearing, ultrasonic treatment and high-pressure homogenization to obtain a mixture. In the present disclosure, the conditions of high-speed shearing preferably include: the rotation speed is 1000 rpm, and the time is 1-2 min. In the present disclosure, the conditions of the ultrasonic treatment preferably include: the time is 2 min, the power is 1000 w, and the frequency is 50 kHz. In the present disclosure, the conditions of high-pressure homogenization preferably include: the time is 4-6 min, and the pressure is 50000 psi.

[0059] In the disclosure, the obtained mixture is freeze-dried to obtain eyebrow growth powder. In the present disclosure, the conditions of freeze-drying process preferably include: pre-freezing the mixture at 10 C. for 4-6 hours, and then freezing at 45 C. and 1 kPa for 24-36 hours.

[0060] The disclosure also provides the use of the traditional Chinese medicine composition or the eyebrow growth powder in preparing a medicine for preventing eyebrow shedding. The disclosure has no special limitation on the dosage form and preparation method of the medicine, and the skilled in the art can adopt routine methods.

[0061] The disclosure also provides the use of the traditional Chinese medicine composition or the eyebrow growth powder in preparing an eyebrow growth medicine. The disclosure has no special limitation on the dosage form and preparation method of the medicine, and the skilled in the art can adopt routine methods.

[0062] In the present disclosure, the application method of the eyebrow growth powder preferably includes: taking 0.3 g of the eyebrow growth powder, adding 10 ml of boiling water, uniformly mixing until the powder is suspended, and cooling to room temperature, that is, the eyebrow growing liquid medicine is obtained, and dipping the eyebrow growing liquid medicine with a cotton swab every day after cleansing, and smearing 2-5 ml along the direction of eyebrow growth.

[0063] In order to further illustrate the present disclosure, the following examples will be described in detail, but they cannot be understood as limiting the scope of protection of the present disclosure.

Example 1

[0064] This example provides a traditional Chinese medicine composition powder for eyebrow enhancement, specifically as follows:

[0065] 5 parts of chuanxiong rhizome, 8 parts of mulberry, 5 parts of sesame, 20 parts of Eclipta Herba, 10 parts of Platycladi cacumen, 5 parts of Rehmanniae Radix Preparata, 3 parts of Gardeniae Fructus and 3 parts of Ligustri Lucidi Fructus.

[0066] The preparation method of the traditional Chinese medicine composition comprises the following:

[0067] S1, chuanxiong rhizome, mulberry, sesame, Eclipta Herba, Platycladi cacumen, Rehmanniae Radix Preparata, Gardeniae Fructus and Ligustri Lucidi Fructus were weighed according to the parts by weight, then washed, drained, dried, After ultra-fine grinding for 60 s, it was sieved through 100 mesh to obtain a mixed powder.

[0068] S2, 35 parts by weight of glycerol tristearate was added into the mixed powder, and heated in a water bath at 80 C. to obtain an oil phase.

[0069] S3, 60 parts by weight of soybean lecithin and 45 parts by weight of poloxamer 188 were weighed, and 5 parts by volume of distilled water was added, stirred and dissolved, and heated in a water bath at 100 C. to obtain a water phase.

[0070] S4, the oil phase prepared by S2 and the water phase prepared by S3 were mixed, and sheared at high speed for 1.5 min at a rotating speed of 1000 rpm; then ultrasonic treated for 2 min, with the power of 1000 w and frequency of 50 kHz; then homogenized under high pressure for 5 min at a pressure of 50000 psi to obtain a mixed solution.

[0071] S5, the mixed solution was put in a rotary evaporator, pre-freezed at 10 C. for 5 h, and the freeze-drying temperature was adjusted to 45 C., the pressure was adjusted to 1 kPa, and the time was adjusted to 30 h, so as to prepare a Chinese medicine freeze-dried powder, namely the eyebrow growth powder.

Example 2

[0072] This example provides a Chinese medicine composition powder for eyebrow growth. Compared with Example 1, the only difference is as follows:

[0073] 2 parts of chuanxiong rhizome, 5 parts of mulberry, 3 parts of sesame, 10 parts of Eclipta Herba, 20 parts of Platycladi cacumen, 5 parts of Rehmanniae Radix Preparata, 5 parts of Gardeniae Fructus and 3 parts of Ligustri Lucidi Fructus.

Example 3

[0074] This example provides a Chinese medicine composition powder for eyebrow growth. Compared with Example 1, the only difference is as follows:

[0075] 5 parts of chuanxiong rhizome, 8 parts of mulberry, 3 parts of sesame, 15 parts of Eclipta Herba, 15 parts of Platycladi cacumen, 3 parts of Rehmanniae Radix Preparata, 3 parts of Gardeniae Fructus and 5 parts of Ligustri Lucidi Fructus.

Example 4

(A) Induction of Growth Period

[0076] In this experiment, the traditional Chinese medicine composition was used as the experimental drug, and cold boiled water was used as the blank control to carry out the hair growth experiment of mice. The specific experimental operation is as follows. [0077] (1) The mice were divided into groups and treated with drugs.

[0078] C57BL/6J mice purchased by Guangdong Zhiyuan Biomedical Technology Co., Ltd. were used as experimental animals. At first, Weiting depilatory cream (from France, produced by Reckitt Benckiser (China) Co., Ltd.) was used to shave the back hair of each mouse, so that the skin on the back was bare and pink. Then the experimental mice were divided into three groups (blank control group, eyebrow powder group prepared in Example 1 and Platycladi cacumen group), with 7 mice in each group. The experimental grouping is shown in FIG. 2.

[0079] Medication: 0.3 g raw eyebrow powder was taken and added into 10 ml ultrapure water, and stirred to make it into suspension. After cooling to room temperature, the liquid medicine was dipped with a cotton swab and smeared on the back of mice. The daubing frequency of the three groups of mice was once a day, each time was about 1 ml. The whole depilation area on the back of mice was divided into three parts, namely, upper, middle and lower parts, which were administered separately, while the blank control group was daubed with the same amount of cold boiled water. [0080] (2) Experimental result

[0081] The experimental results are shown in FIGS. 3-7. From left to right, there are blank group, compound medicine group (eyebrow powder prepared in Example 1) and Platycladi cacumen group. The results on the first day of administration (Jun. 1, 2023) are shown in FIG. 3, from which it can be seen that there is no change in the three groups of mice on the first day of administration. The results of the eighth day of administration (Jun. 8, 2023) are shown in FIG. 4. As can be seen from FIG. 4, the upper part of the back of the mice in the compound medicine group of the present disclosure began to turn gray, while the other two groups had no obvious changes. The results on the 10th day of administration (Jun. 10, 2023) are shown in FIG. 5. From FIG. 5, it can be seen that the lower part of the back of the mice in the compound medicine group of the present disclosure began to turn gray, and the upper part began to turn black, while the other two groups had no obvious changes. The results on the 12th day of administration (Jun. 12, 2023) are shown in FIG. 6. From FIG. 6, it can be seen that the lower part of the back of the mice in the compound medicine group of the present disclosure is gray, and the upper part begins to grow short hair, while the back of the Platycladi cacumen group begins to turn gray, and there is no obvious change in the blank group. The results on the 15th day of administration (Jun. 15, 2023) are shown in FIG. 7. From FIG. 7, it can be seen that black hair has grown on the back of the mice in the compound medicine group of the present disclosure, the back of the Platycladi cacumen group began to turn black and the blackened area expanded, and the skin of the blank group began to turn gray.

(B) Clinical Trials

[0082] There were 10 volunteers with sparse eyebrows. After cleaning their faces every night, 2-5 ml of eyebrow growth liquid (0.3 g eyebrow growth powder and 10 ml boiled water) was applied along the direction of eyebrow growth. The trial period was 21 days. The length, density, gloss and patient satisfaction of eyebrows before and after treatment were observed. The length, density and gloss were evaluated by 0-2 points (poor), 3-4 points (general), 5-6 points (good) and 7-8 points (very good) respectively. The patient satisfaction was evaluated by ten points. The higher the score, the higher the patient satisfaction. The results are shown in Table 1.

TABLE-US-00001 TABLE 1 Post-use Scoring Table Eyebrow Growth Index Patient Group Length Density Gloss satisfaction 1 4 4 6 9 2 6 7 7 8 3 5 5 5 9 4 6 6 8 9 5 7 5 4 10 6 8 8 5 9 7 6 4 6 9 8 8 8 8 10 9 7 5 7 8 10 5 6 6 10

[0083] Eyebrow length: (normally it grows about 0.2-0.5 mm every day, and it grows about 1-1.5 cm a month). [0084] 0-1 points: the eyebrow growth length is about 0-1 mm; [0085] 2-4 points: the eyebrow growth length is about 2-3 mm; [0086] 5-7 points: the eyebrow growth length is about 4-8 mm; [0087] 8-10 points: the eyebrow growth length is about 8 mm or more.

Eyebrow Density:

[0088] 0-1 points: extremely sparse, with less than 10 hairs in a single visual field; [0089] 2-4 points: sparse, clearly visible skin, with less than 20 hairs in a single visual field; [0090] 5-7 points: medium density, almost invisible skin, the number of hairs in a single visual field is 20, <30; [0091] 8-10 points: dense, the skin is invisible, and the number of hairs in a single visual field is 30.

Eyebrow Gloss:

[0092] 0-1 points: dull; [0093] 2-4 points: average luster; [0094] 5-7 points: the hair is healthy and shiny; [0095] 8-10 points: the hair is black, bright and shiny.

Patient Satisfaction:

[0096] 0-3 points: the drug is irritating after using the medicine, and the hair-increasing effect is average; [0097] 4-8 points: there is no irritation after using the medicine, and the hair-increasing effect is satisfactory; [0098] 9-10 points: there is no irritation after using the medicine, and the hair-increasing effect is very satisfactory.

[0099] All the data were entered into Excel for proofreading, and SPSS statistical software was used to process the data. The counting data were described by rate, and .sup.2 test was used for comparison between groups. The measurement data were described by xs, and t test was used for comparison between groups. P<0.05 is statistically significant.

[0100] Although the above embodiment has described the invention in detail, it is only a part of the embodiment of the invention, not the whole embodiment. Skilled in the art can also obtain other embodiments according to this embodiment without inventing, and these embodiments all belong to the scope of protection of the invention.