Acetylcholinesterase inhibitors and promnesiant serotonin 5-HT4 receptor agonists, their methods of preparation and the pharmaceutical compositions containing the same
09663465 ยท 2017-05-30
Assignee
Inventors
- Patrick Dallemagne (Saint Georges D'Aunay, FR)
- Christophe Rochais (Caen, FR)
- Cedric Lecoutey (Caen, FR)
- Michel Boulouard (Douvre la Delivrande, FR)
- Thomas Freret (Amaye sur Orne, FR)
Cpc classification
A61P25/28
HUMAN NECESSITIES
C07D211/26
CHEMISTRY; METALLURGY
C07D211/20
CHEMISTRY; METALLURGY
C07D211/34
CHEMISTRY; METALLURGY
International classification
C07D211/34
CHEMISTRY; METALLURGY
C07D211/20
CHEMISTRY; METALLURGY
Abstract
Compounds are provided according to Formula (I) ##STR00001##
as well as their enantiomers and their racemics, their acid salts, their hydrates or their solvation products. Among a large number of possible meanings, X represents a halogen, Y an oxygen atom; all of the coefficients m, n, r and s have the value 1, R represents an ethyl and R a cycloalkyl. The invention also includes Methods of preparing the above compounds and the pharmaceutical compositions containing them also are provided.
Claims
1. A compound of the general formula (I): ##STR00008## wherein: X represents a hydrogen atom, a halogen atom (Hal), where (Hal) is fluorine, chlorine, bromine or iodine, or a straight- or branched-chain C.sub.pHal.sub.2p+1 polyhalogenoalkyl group, where p=1, 2, 3 or 4, (Hal) having the same meaning as indicated above; Y represents an oxygen atom, a sulfur atom, or an NR radical where R represents a hydrogen atom, an OH radical or a straight- or branched-chain C.sub.qH.sub.2q+1 alkyl radical, where q=1, 2, 3 or 4; m has a value of 1, 2 or 3; n has a value of 0, 1, 2 or 3; r and s have values selected from r=s=0; r=s=1; r=s=2; r=0 and s=1; and r=0 and s=2; R represents a hydrogen atom, a straight- C1-C5 alkyl group, a branched-chain C1-C5 alkyl group, a straight- C1-C5 fluoro-alkyl group, or branched-chain C1-C5 fluoro-alkyl group; R represents a C3-C10 cycloalkyl or heterocyclic alkyl group, optional substituted by R, where the heteroatom is oxygen, nitrogen, or sulfur; or a C5-C13 bicyclicalkyl or heterobicyclic alkyl group, optionally substituted by R, where the heteroatom is oxygen, nitrogen, or sulfur.
2. The compound of claim 1, wherein X in the formula (I) represents a halogen atom.
3. The compound of claim 1, Y in the formula (I) represents an oxygen atom.
4. The compound of claim 1, wherein all of the coefficients n, r and s in the formula (I) have the value 1.
5. The compound of claim 1, wherein R in the formula (I) represents H, CH.sub.3, CH.sub.2CH.sub.3 or CH.sub.2CH.sub.2F.
6. The compound of claim 1, wherein R in the formula (I) represents a radical taken from the group formed by the radicals cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and 4-piperidine.
7. The compound of claim 2, wherein R is a methyl radical and R is a C.sub.4-C.sub.7 cycloalkyl radical.
8. A pharmaceutical composition that comprises, as an active ingredient, at least one compound according to claim 1.
9. The pharmaceutical composition of claim 8, wherein the pharmaceutical composition contains a pharmaceutically acceptable excipient.
10. The pharmaceutical composition of claim 8, wherein the pharmaceutical composition further comprises a second active ingredient having an inhibitory action of acetylcholinesterase and chosen from the group consisting of: a) 1,2,3,4-Tetrahydroacridin-9-amine, b) (RS)-2-[(1-benzyl-4-piperidyl)methyl-5,6-dimethoxy-2,3-dihydroinden-1-one, c) (S)N-ethyl-N-methyl-3-[(1-dimethylamino)ethyl]-phenyl carbamate, and d) 4aS,6R,8aS)-5,6,9,10,11,12-hexahydro-3-methoxy-11-methyl-4aH-[1]benzofuro [3a,3,2-ef][2]benzazepin-6-ol.
11. The pharmaceutical composition of claim 8, wherein the pharmaceutical composition further comprises at least one partial 5-HT4 receptor agonist chosen from the group consisting of: a) 1-(4-amino-5-chloro-2-methoxyphenyl)-2-[1-butyl-4-piperidyl]propan-1 one, and b) N-(2-(4-(3-(4-amino-5-chloro-3-methoxyphenyl)-3-oxypropyl)piperidin-1-yl)ethyl)methane sulfonamide, the said agonist being in a molar ratio of between 10/90 and 90/10 in relation to the compound according to claim 1.
12. The pharmaceutical composition of claim 8, designed to be administered to mammals and having a promnesiant effect used for the treatment of neurological diseases with amnestic deficit.
13. The pharmaceutical composition of claim 8, that can be used for treatment of Alzheimer's disease in a human subject.
14. The pharmaceutical composition of claim 8 further comprising the compound according to claim 1 in a mixture with dispersion agents, softening agents, suspension agents, sweeteners or flavor enhancers.
Description
BRIEF DESCRIPTION OF THE DRAWING
(1) The FIGURE gives the results obtained on mice for the improvement of memory performance.
DETAILED DESCRIPTION
(2) So as better to understand the subject matter of the invention and illustrate it in a non-limiting way, a certain number of examples will now be described. To facilitate identification of the different compounds illustrated in the examples, there now follows Table 1, corresponding to the compounds of formula (I), Table 2 corresponding to the compounds of formula (II) and Table 3 corresponding to the compounds of formula (III), each of these tables giving the meanings of the different substituents for each of the examples provided in the text below. The numbers appearing in these examples correspond to the compounds bearing the same numbers respectively in the above tables. Figures that appear in the text as superscripted, correspond to references used to implement the examples.
(3) TABLE-US-00001 TABLE 1 Compounds of formula (I) (I)
(4) TABLE-US-00002 TABLE 2 Compounds of Formula (II) (II)
(5) TABLE-US-00003 TABLE 3 Compounds of Formula (III) (III)
References for implementing the examples: .sup.1 Eustache, F., Desgranges, B. Concepts et modles en neuropsychologie de la mmoire. (2003). In Meulemans, T., Desgranges, B., Adam, S., Eustache, F. (eds.). Evaluation et prise en charge des troubles mnsiques. Marseille: Solal. .sup.1 Mingaud F., Mormede C., Etchamendy N., Mons N., Niedergang B., Wietrzych M., Pallet V., Jaffard R., Krezel W., Higueret P., Marighetto A., 2008 J. Neurosci. 28, 279-291. .sup.1 Levallet G, Hotte M, Boulouard M, Dauphin F (2009) Psychopharmacology (Berl). 202:125-39. .sup.1 Moser, P. C., Bergis, O. E., Jegham, S., Lochead, A., Duconseille, E., Terranova, J. P. J Pharmaco Exp Ther 2002; 302:731-741. .sup.1 Birks, J. Cochrane Database Syst. Rev. 2006, CD005593. .sup.1 Lezoualc'h, F. Exp. Neurol. 2007, 205, 325-329. .sup.1 Holzgrabe, U.; Kapkova, P.; Alptuzun, V.; Scheiber, J.; Kugelmann, E. Expert Opin. Ther. Targets 2007, 11, 161-179. .sup.1 Clark, R. D.; Eglen, R.; Jahangir, A.; Miller, A. B.; Gardner, J. O. PCT Int. Appl. 1994, WO9427965 .sup.1 Sonda, S.; Kawahara, T.; Murozono, T.; Sato, N.; Asano, K.; Haga, K. Bioorg. Med. Chem. 2003, 11(19), 4225-4234 .sup.1 Yang, W.; Ruan, Z.; Wang, Y.; Van Kirk, K.; Ma, Z.; Arey, B. J.; Cooper, C. B.; Seethala, R.; Feyen, J. H. M.; Dickson Jr, J. K. J. Med. Chem. 2009, 52 (4), 1204-1208 .sup.1 Kaspi, J.; Lerman, O.; Arad, O.; Alnabari, M.; Sery, Y. Eur. Pat. Appl. 2004, 1386607
Example 1
1-(4-amino-2-methoxyphenyl)-3-[1-butyl-4-piperidyl]propan-1-one
(6) 1 ml of TFA is added to 184 mg of tert-butyl 4-[3-(4-amino-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate (0.51 mmol) in solution in 1 ml of DCM. The reaction medium is stirred at AT for 15 minutes then concentrated in a rotary evaporator under reduced pressure. The residue is taken up in saturated NaHCO.sub.3 then extracted 3 times with AcOEt. The organic phases are combined then washed in brine. The organic phase is then dried and concentrated to obtain 42 mg of deprotected product. This is directly engaged in the alkylation reaction by dissolving it in 3 ml of DMF to which is added 28 mg of K.sub.2CO.sub.3 (0.21 mmol) and 20 L of iodobutane (0.18 mmol). The new reaction medium is held at 110 C. for 2 h. After dilution in AcOEt, the mixture is washed 4 times in brine, dried over MgSO.sub.4 then concentrated. The residue obtained is purified over a silica gel column (DCM/AcOEt 10/0 to 0/10 then MeOH 5%). The product obtained has a yield of 22%.
(7) C.sub.19H.sub.30N.sub.2O.sub.2
(8) MP=80 C.
(9) NMR .sup.1H (CDCl.sub.3): 0.89 (t, J=7.3 Hz, 3H, CH.sub.3), 1.27 (m, 5H, 3 CH, CH.sub.2 BUT), 1.45 (m, 2H, CH.sub.2 BUT), 1.56 (m, 2H), 1.68 (m, 2H), 1.85 (m, 2H, 2 CHN), 2.27 (m, 2H, CH.sub.2N), 2.90 (m, 4H, CH.sub.2, 2 CHN), 3.83 (s, 3H, CH.sub.3), 4.03 (s, 2H, NH.sub.2), 6.13 (d, J=2.0 Hz, 1H, H.sub.Ar), 6.24 (dd, J=8.7 Hz, J=2.0 Hz, 1H, H.sub.Ar), 7.69 (d, J=8.7 Hz, 1H, H.sub.Ar).
(10) IR (KBr, cm.sup.1): 3438.2, 3255.4, 3228.4, 2952.1, 2926.8, 2932.8, 2856.9, 1726.5, 1640.7, 1594.1, 1468.1, 1434.3, 1272.4, 1259.8, 1212.6, 1177.1, 1030.2.
Example 2
1-(4-amino-5-bromo-2-methoxyphenyl)-3-[1-butyl-4-piperidyl]propan-1-one
(11) Compound 3 is prepared according to the operating method of Example 1 described above, considering the following quantities:
(12) tert-butyl 4-[3-(4-amino-5-bromo-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 90 mg (0.19 mmol)
(13) Trifluoroacetic acid: 1 ml
(14) DCM: 1 ml
(15) iodobutane: 26 L (0.23 mmol)
(16) K.sub.2CO.sub.3: 34 mg (0.25 mmol)
(17) DMF: 4 ml
(18) The expected compound is obtained with a yield of 50% and is in the form of a pale yellow powder.
(19) It has the following characteristics:
(20) C.sub.19H.sub.29BrN.sub.2O.sub.2
(21) MP=205 C.
(22) NMR .sup.1H (CDCl.sub.3): 0.91 (t, J=7.3 Hz, 3H, CH.sub.3), 1.29 (m, 5H, 3 CH, CH.sub.2 BUT), 1.48 (m, 2H, CH.sub.2 BUT), 1.58 (m, 2H), 1.70 (m, 2H), 1.89 (m, 2H, 2 CHN), 2.31 (m, 2H, CH.sub.2N), 2.91 (m, 4H, CH.sub.2, 2 CHN), 3.84 (s, 3H, CH.sub.3), 4.55 (s, 2H, NH.sub.2), 6.27 (s, 1H, H.sub.Ar), 7.93 (s, 1H, H.sub.Ar).
(23) IR (KBr, cm.sup.1): 3436.2, 3255.8, 3156.4, 2961.4, 2932.8, 2873.5, 2633.0, 2516.0, 1725.7, 1655.3, 1619.3, 1584.6, 1461.4, 1417.0, 1300.1, 1253.4, 1216.2, 1179.2, 1044.2.
Example 3
1-(4-amino-5-iodo-2-methoxyphenyl)-3-[1-butyl-4-piperidyl]propan-1-one
(24) Compound 4 is prepared according to the operating method of Example 1 described above, considering the following quantities:
(25) tert-butyl 4-[3-(4-amino-5-iodo-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 124 mg (0.25 mmol)
(26) Trifluoroacetic acid: 1 ml
(27) DCM: 1 ml
(28) iodobutane: 28 L (0.25 mmol)
(29) K.sub.2CO.sub.3: 37 mg (0.27 mmol)
(30) DMF: 4 ml
(31) The expected compound is obtained with a yield of 45% and is in the form of a pale yellow powder.
(32) It has the following characteristics:
(33) C.sub.19H.sub.29IN.sub.2O.sub.2
(34) MP=130 C.
(35) NMR .sup.1H (CDCl.sub.3): 0.91 (t, J=7.8 Hz, 3H, CH.sub.3), 1.27 (m, 5H, 3 CH, CH.sub.2 BUT), 1.47 (m, 2H, CH.sub.2 BUT), 1.59 (m, 2H), 1.70 (m, 2H), 1.87 (m, 2H, 2 CHN), 2.29 (m, 2H, CH.sub.2N), 2.91 (m, 4H, CH.sub.2, 2 CHN), 3.84 (s, 3H, CH.sub.3), 4.49 (s, 2H, NH.sub.2), 6.25 (s, 1H, H.sub.Ar), 8.11 (s, 1H, H.sub.Ar).
(36) IR (KBr, cm.sup.1): 3453.8, 3342.7, 3209.3, 2952.1, 2927.6, 2860.0, 1621.7, 1577.9, 1468.1, 1450.2, 1412.1, 1262.9, 1216.4, 1177.1, 1040.6.
Example 4
1-(4-amino-2-methoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one
(37) Compound 1 is prepared according to the operating method of Example 1 described above, considering the following quantities:
(38) tert-butyl 4-[3-(4-amino-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 310 mg (0.86 mmol) Trifluoroacetic acid: 2 ml
(39) DCM: 2 ml
(40) (iodomethyl)cyclohexane: 87 L (0.63 mmol)
(41) K.sub.2CO.sub.3: 102 mg (0.74 mmol)
(42) DMF: 5 ml
(43) The expected compound is obtained with a yield of 39% and is in the form of a yellow powder.
(44) It has the following characteristics:
(45) MP=90 C.
(46) C.sub.22H.sub.34N.sub.2O.sub.2
(47) NMR .sup.1H (CDCl.sub.3): 0.87 (m, 2H, 2 CH), 1.18 (m, 6H, 6 CH), 1.47 (m, 1H, CH), 1.64 (m, 7H, 5 CH, CH.sub.2), 1.75 (m, 2H, 2 CH), 1.81 (m, 2H, 2 CHN), 2.08 (d, J=7.1 Hz, 2H, CH.sub.2N), 2.85 (m, 2H, 2 CHN), 2.90 (m, 2H, CH.sub.2), 3.82 (s, 3H, CH.sub.3), 4.09 (s, 2H, NH.sub.2), 6.16 (d, 1=2.0 Hz, 1H, H.sub.Ar), 6.25 (dd, J=8.5 Hz, J=2.0 Hz, 1H, H.sub.Ar), 7.70 (d, J=8.5 Hz, 1H, H.sub.Ar,).
(48) IR (KBr, cm.sup.1): 3447.7, 3366.5, 3245.1, 2922.8, 2847.0, 2809.3, 2770.5, 1590.7, 1601.8, 1508.7, 468.1, 1448.3, 1429.4, 1347.3, 1310.9, 1272.3, 1257.2, 1218.0, 1206.9, 1134.0, 1122.8, 1031.4, 823.1.
Example 5
1-(4-amino-5-fluoro-2-methoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one
(49) 1 ml of TFA is added to 72 mg of tert-butyl 4-[3-(4-amino-5-fluoro-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate (0.19 mmol) in solution in 1 ml of DCM. The reaction medium is stirred at AT for 15 minutes then concentrated in a rotary evaporator under reduced pressure. The residue is dissolved in 3 ml of DMF to which is added 262 mg of K.sub.2CO.sub.3 (1.89 mmol) and 35 L of iodomethylcyclohexane (0.25 mmol). The new reaction medium is held at 110 C. for 3 h. After dilution in AcOEt, the mixture is washed 4 times in brine, dried over MgSO.sub.4 then concentrated. The residue obtained is purified over a silica gel column (elution gradient: (DCM/AcOEt 10/0 to 0/10 then DCM+2% Et.sub.3N). The expected product is obtained with a yield of 56%.
(50) Appearance: yellow solid
(51) MP=97 C.
(52) C.sub.22H.sub.33FN.sub.2O.sub.2
(53) NMR .sup.1H (CDCl.sub.3): 0.86 (m, 2H, 2 CH), 1.20 (m, 6H, 6 CH), 1.48 (m, 1H, CH), 1.64 (m, 7H, 5 CH, CH.sub.2), 1.74 (m, 2H, 2 CH), 1.82 (m, 2H, 2 CHN), 2.08 (d, J=7.1 Hz, 2H, CH.sub.2N), 2.85 (m, 2H, 2 CHN), 2.91 (m, 2H, CH.sub.2), 3.84 (s, 3H, CH.sub.3), 4.14 (s, 2H, NH.sub.2), 6.27 (d, J.sub.H-F=7.1 Hz, 1H, H.sub.Ar), 7.55 (d, J.sub.H-F=12.2 Hz, 1H, H.sub.Ar).
(54) IR (KBr, cm.sup.1): 3352.5, 3220.5, 2921.4, 2849.5, 2801.2, 2768.3, 1626.5, 1601.8, 1520.8, 1467.2, 1449.7, 1428.3, 1357.0, 1310.9, 1250.5, 1207.2, 1171.0, 1148.3, 1028.9, 823.8.
Example 6
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cyclopropylmethyl)-4-piperidyl]propan-1-one
(55) Compound 6 is prepared according to the operating method of Example 5 described above, considering the following quantities:
(56) tert-butyl 4-[3-(4-amino-5-chloro-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate:.sup.v 159 mg (0.40 mmol)
(57) Trifluoroacetic acid: 2 ml
(58) DCM: 2 ml
(59) (iodomethyl)cyclopropane: 43 mg (0.48 mmol)
(60) K.sub.2CO.sub.3: 553 mg (4.00 mmol)
(61) DMF: 4 ml
(62) The expected compound is obtained with a yield of 39% and is in the form of a yellow powder.
(63) MP=162 C.
(64) C.sub.19H.sub.27ClN.sub.2O.sub.2
(65) NMR .sup.1H (CD.sub.3OD): 0.18 (m, 2H), 0.58 (m, 2H), 0.94 (m, 1H), 1.34 (m, 3H), 1.61 (m, 2H), 1.79 (m, 2H), 2.10 (m, 2H), 2.33 (m, 2H), 2.95 (m, 2H), 3.15 (m, 2H), 3.89 (s, 3H, CH.sub.3), 6.31 (s, 1H, H.sub.Ar), 7.51 (s, 1H, H.sub.Ar).
(66) IR (KBr, cm.sup.1): 3466.5, 3349.8, 2923.7, 2849.4, 2804.8, 2779.7, 1644.0, 1621.5, 1586.4, 1500.8, 1465.2, 1419.9, 1376.9, 1313.2, 1252.0, 1215.3, 1177.9, 1118.6, 825.7.
Example 7
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cyclobutylmethyl)-4-piperidyl]propan-1-one
(67) Compound 7 is prepared according to the operating method of Example 5 described above, considering the following quantities:
(68) tert-butyl 4-[3-(4-amino-5-chloro-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 159 mg (0.40 mmol)
(69) Trifluoroacetic acid: 2 ml
(70) DCM: 2 ml
(71) (iodomethyl)cyclobutane: 34 L (0.30 mmol)
(72) K.sub.2CO.sub.3: 45 mg (4.00 mmol)
(73) DMF: 4 ml
(74) The expected compound is obtained with a yield of 45% and is in the form of a yellow powder.
(75) MP=143 C.
(76) C.sub.20H.sub.29ClN.sub.2O.sub.2
(77) NMR .sup.1H (CDCl.sub.3): 1.25 (m, 3H, 3 CH), 1.57 (m, 2H, CH.sub.2), 1.78 (m, 8H, 4 CH.sub.BUT, 2 CH, 2 CHN), 2.06 (m, 2H, 2 CH.sub.BUT), 2.37 (d, J=6.6 Hz, 2H, CH.sub.2N), 2.54 (m, 1H, CH.sub.BUT), 2.83 (m, 2H, 2 CHN), 2.90 (m, 2H, CH.sub.2), 3.85 (s, 3H, CH.sub.3), 4.42 (s, 2H, NH.sub.2), 6.25 (s, 1H, H.sub.Ar), 7.78 (s, 1H, H.sub.Ar).
(78) IR (KBr, cm.sup.1): 3484.7, 3226.9, 2923.5, 2852.3, 2804.8, 2763.1, 1642.3, 1623.5, 1574.6, 1453.8, 1420.7, 1397.2, 1306.3, 1257.4, 1214.1, 1175.4, 1011.2, 830.4.
Example 8
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cyclopentylmethyl)-4-piperidyl]propan-1-one
(79) Compound 8 is prepared according to the operating method of Example 1 described above, considering the following quantities:
(80) tert-butyl 4-[3-(4-amino-5-chloro-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 134 mg (0.34 mmol)
(81) Trifluoroacetic acid: 2 ml
(82) DCM: 2 ml
(83) (iodomethyl)cyclopentane: 57 mg (0.27 mmol)
(84) K.sub.2CO.sub.3: 41 mg (0.29 mmol)
(85) DMF: 3 ml
(86) The expected compound is obtained with a yield of 35% and is in the form of a yellow powder.
(87) MP=142 C.
(88) C.sub.21H.sub.31ClN.sub.2O.sub.2
(89) NMR .sup.1H (CDCl.sub.3): 1.21 (m, 5H, 2 CH.sub.PENT, 3 CH), 1.50 (m, 2H, 2 CH.sub.PENT), 1.60 (m, 6H, 2 CH.sub.PENT, CH.sub.2, 2 CH), 1.76 (m, 2H, 2 CH.sub.PENT), 1.86 (m, 2H, 2 CHN), 2.05 (m, 1H, CH.sub.PENT), 2.24 (d, J=7.1 Hz, 2H, CH.sub.2N), 2.90 (m, 4H, CH.sub.2, 2 CHN), 3.85 (s, 3H, CH.sub.3), 4.44 (s, 2H, NH.sub.2), 6.26 (s, 1H, H.sub.Ar), 7.79 (s, 1H, H.sub.Ar).
(90) IR (KBr, cm.sup.1): 3485.6, 3332.1, 2936.2, 2923.8, 2860.0, 2799.7, 2768.0, 1643.8, 1622.7, 1599.5, 1574.2, 1453.4, 1421.6, 1310.5, 1266.0, 1214.2, 1175.8, 1018.4, 831.0.
Example 9
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one
(91) Compound 9 is prepared according to the operating method of Example 1 described above, considering the following quantities:
(92) tert-butyl 4-[3-(4-amino-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 953 mg (2.41 mmol)
(93) Trifluoroacetic acid: 2 ml
(94) DCM: 2 ml
(95) (bromomethyl)cyclohexane: 262 L (1.88 mmol)
(96) K.sub.2CO.sub.3: 281 mg (2.03 mmol)
(97) DMF: 4 ml
(98) The expected compound is obtained with a yield of 44% and is in the form of a pale yellow solid.
(99) It has the following characteristics:
(100) MP=154 C.
(101) C.sub.22H.sub.33ClN.sub.2O.sub.2
(102) NMR .sup.1H (CDCl.sub.3): 0.85 (m, 2H, 2 CH), 1.20 (m, 6H, 6 CH), 1.47 (m, 1H, CH), 1.68 (m, 11H, 7 CH, 2 CHN, CH.sub.2), 2.08 (d, J=6.8 Hz, 2H, CH.sub.2N), 2.84 (m, 2H, 2 CHN), 2.89 (m, 2H, CH.sub.2), 3.85 (s, 3H, CH.sub.3), 4.44 (s, 2H, NH.sub.2), 6.26 (s, 1H, H.sub.Ar), 7.79 (s, 1H, H.sub.Ar).
(103) IR (KBr, cm.sup.1): 3484.8, 3229.8, 2920.7, 2850.0, 2803.0, 2768.0, 1640.7, 1623.0, 1575.0, 1452.8, 1420.9, 1313.8, 1255.6, 1214.3, 1177.2, 1012.7, 831.0.
Example 10
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]-2 methylpropan-1-one
(104) Compound 10 is prepared according to the operating method of Example 5 described above, considering the following quantities:
(105) tert-butyl 4-[3-(4-amino-5-chloro-2-methoxy)-2-methyl-3-oxopropyl]piperidine-1-carboxylate: 50 mg (0.12 mmol)
(106) Trifluoroacetic acid: 5004
(107) DCM: 2 ml
(108) (bromomethyl)cyclohexane: 20 L (0.15 mmol)
(109) K.sub.2CO.sub.3: 168 mg (1.22 mmol)
(110) DMF: 1 ml
(111) The expected compound is obtained with a yield of 42% and is in the form of a yellow oil.
(112) C.sub.23H.sub.35ClN.sub.2O.sub.2
(113) NMR .sup.1H (CDCl.sub.3): 0.86 (m, 2H, 2 CH), 1.05 (d, J=6.8 Hz, 3H, CH.sub.3), 1.19 (m, 6H, 6 CH), 1.49 (m, 1H, CH), 1.60 (m, 7H, 5 CH, CH.sub.2), 1.73 (m, 2H, 2 CH), 1.86 (m, 2H, 2 CHN), 2.15 (d, J=6.6 Hz, 2H, CH.sub.2N), 2.85 (m, 2H, 2 CHN), 3.57 (qd, 1=6.8 Hz, 1H, CH), 3.81 (s, 3H, CH.sub.3), 4.42 (s, 2H, NH.sub.2), 6.24 (s, 1H, H.sub.Ar), 7.67 (s, 1H, H.sub.Ar).
(114) IR (KBr, cm.sup.1): 3477.5, 3351.5, 2922.4, 2851.8, 2801.6, 2766.2, 1650.4, 1621.4, 1590.8, 1464.5, 1450.3, 1417.3, 1305.1, 1255.8, 1214.8, 1178.2, 1007.6.
Example 11
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-[(2-methylcyclohexyl)methyl]-4-piperidyl] propan-1-one
(115) Compound 11 is prepared according to the operating method of Example 5 described above, considering the following quantities:
(116) tert-butyl 4-[3-(4-amino-5-chloro-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 175 mg (0.44 mmol)
(117) Trifluoroacetic acid: 1.5 ml
(118) DCM: 3 ml
(119) (iodomethyl)-2-methylcyclohexane: 127 mg (0.53 mmol)
(120) K.sub.2CO.sub.3: 610 mg (4.42 mmol)
(121) DMF: 4 ml
(122) The expected compound is obtained with a yield of 45% and is in the form of a yellow solid.
(123) MP=111 C.
(124) C.sub.23H.sub.35ClN.sub.2O.sub.2
(125) NMR .sup.1H (CDCl.sub.3): 0.83 (d, J=7.1 Hz, 3H, CH.sub.3), 1.28 (m, 7H, 7 CH), 1.60 (m, 8H, 6 CH, CH.sub.2), 1.66 (m, 2H, 2 CH), 1.86 (m, 2H, 2 CHN), 2.13 (d, J=6.8 Hz, 2H, CH.sub.2N), 2.89 (m, 4H, CH.sub.2, 2 CHN), 3.84 (s, 3H, CH.sub.3), 4.49 (s, 2H, NH.sub.2), 6.27 (s, 1H, H.sub.Ar), 7.78 (s, 1H, H.sub.Ar).
(126) IR (KBr, cm.sup.1): 3482.4, 3336.7, 3209.3, 2921.4, 2850.5, 2803.8, 2771.2, 1640.7, 1618.5, 1585.3, 1466.1, 1453.4, 1421.6, 1310.5, 1253.4, 1215.0, 1180.3, 1018.4.
Example 12
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cycloheptylmethyl)-4-piperidyl]propan-1-one
(127) Compound 12 is prepared according to the operating method of Example 5 described above, considering the following quantities:
(128) tert-butyl 4-[3-(4-amino-5-chloro-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 152 mg (0.38 mmol)
(129) Trifluoroacetic acid: 1 ml
(130) DCM: 2 ml
(131) (iodomethyl)cycloheptane: 110 mg (0.46 mmol)
(132) K.sub.2CO.sub.3: 535 mg (3.84 mmol)
(133) DMF: 4 ml
(134) The expected compound is obtained with a yield of 42% and is in the form of a yellow solid.
(135) MP=148 C.
(136) C.sub.23H.sub.35ClN.sub.2O.sub.2
(137) NMR .sup.1H (CDCl.sub.3): 1.12 (m, 2H, 2 CH), 1.50 (m, 18H, CH.sub.2, 16 CH), 1.95 (m, 2H, 2 CHN), 2.15 (m, 2H, CH.sub.2N), 2.89 (m, 4H, CH.sub.2, 2 CHN), 3.85 (s, 3H, CH.sub.3), 4.49 (s, 2H, NH.sub.2), 6.27 (s, 1H, H.sub.Ar), 7.78 (s, 1H, H.sub.Ar).
(138) IR (KBr, cm.sup.1): 3481.0, 3247.7, 2919.6, 2850.2, 2800.8, 2768.1, 1637.6, 1621.7, 1582.3, 1453.6, 1419.5, 1307.1, 1252.8, 1214.4, 1176.1, 1012.6.
Example 13
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-[(piperidin-4-yl)methyl]-4-piperidyl] propan-1-one dichlorohydrate
(139) The intermediate, resulting in compound 13, is prepared according to the operating method of Example 1 described above, considering the following quantities:
(140) tert-butyl 4-[3-(4-amino-5-chloro-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 212 mg (0.53 mmol)
(141) Trifluoroacetic acid: 1 ml
(142) DCM: 2 ml
(143) tert-butyl 4-(iodomethyl)piperidine-1-carboxylate: 124 mg (0.38 mmol)
(144) K.sub.2CO.sub.3: 62 mg (0.45 mmol)
(145) DMF: 4 ml
(146) This intermediate is obtained with a yield of 24% and is in the form of an oil.
(147) 40 mg of this intermediate (0.09 mmol) are then dissolved in 5 ml of EtOH to which 300 ml of concentrated HCl are added. After 3 hours of stirring at ambient temperature, the solution is concentrated in a rotary evaporator. Toluene is added to make an azeotropic mixture with the remaining residues of water. The dry residue obtained is then taken up in ethyl ether then filtered. 30 mg of expected product are then obtained with a yield of 80%
(148) MP>260 C.
(149) C.sub.21H.sub.34Cl.sub.3N.sub.3O.sub.2
(150) NMR .sup.1H (DMSO-d6): 1.38 (m, 2H, 2 CH), 1.46 (m, 3H, CH, CH.sub.2), 1.63 (m, 2H, 2 CH), 1.76 (m, 2H, 2 CH), 1.96 (m, 2H, 2 CH), 2.11 (m, 1H, CH), 2.81 (m, 6H, 4 CHN, CH.sub.2), 2.91 (m, 2H, CH.sub.2N), 3.22 (m, 2H, 2 CHN), 3.45 (m, 2H, 2 CHN), 3.79 (s, 3H, CH.sub.3), 6.46 (s, 1H, H.sub.Ar), 7.53 (s, 1H, H.sub.Ar), 9.03 (m, 2H, 2 NH), 10.2 (m, 1H, NH).
(151) IR (KBr, cm.sup.1): 3390.0, 3295.6, 3194.0, 2938.4, 2731.7, 1660.0, 1625.1, 1592.8, 1464.5, 1449.1, 1417.2, 1316.4, 1262.8, 1249.0, 1212.0, 1180.4.
Example 14
1-(4-amino-5-bromo-2-methoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one
(152) Compound 14 is prepared according to the operating method of Example 1 described above, considering the following quantities:
(153) tert-butyl 4-[3-(4-amino-5-bromo-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 65 mg (0.19 mmol)
(154) Trifluoroacetic acid: 1 ml
(155) DCM: 1 ml
(156) (bromomethyl)cyclohexane: 32 L (0.23 mmol)
(157) K.sub.2CO.sub.3: 34 mg (0.25 mmol)
(158) DMF: 4 ml
(159) The expected compound is obtained with a yield of 60% and is in the form of a pale yellow powder.
(160) MP=148 C.
(161) C.sub.22H.sub.33BrN.sub.2O.sub.2
(162) NMR .sup.1H (CDCl.sub.3): 0.87 (m, 2H, 2 CH), 1.20 (m, 6H, 6 CH), 1.48 (m, 1H, CH), 1.60 (m, 7H, 5 CH, CH.sub.2), 1.74 (m, 2H, 2 CH), 1.81 (m, 2H, 2 CHN), 2.09 (d, J=6.8 Hz, 2H, CH.sub.2N), 2.87 (m, 4H, 2 CHN, CH.sub.2), 3.84 (s, 3H, CH.sub.3), 4.51 (s, 2H, NH.sub.2), 6.26 (s, 1H, H.sub.Ar), 7.93 (s, 1H, H.sub.Ar).
(163) IR (KBr, cm.sup.1): 3471.6, 3224.0, 2920.9, 2851.8, 2801.6, 2766.2, 1634.4, 1621.5, 1580.7, 1449.7, 1417.3, 1302.3, 1260.8, 1216.5, 1178.2, 1042.4.
Example 15
1-(4-amino-5-iodo-2-methoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one
(164) Compound 12 is prepared according to the operating method of Example 1 described above, considering the following quantities:
(165) tert-butyl 4-[3-(4-amino-5-iodo-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 110 mg (0.23 mmol)
(166) Trifluoroacetic acid: 2 ml
(167) DCM: 2 ml
(168) (bromomethyl)cyclohexane: 36 L (0.26 mmol)
(169) K.sub.2CO.sub.3: 33 mg (0.24 mmol)
(170) DMF: 2 ml
(171) The expected compound is obtained with a yield of 42% and is in the form of a yellow powder.
(172) It has the following characteristics:
(173) MP=178-180 C.
(174) C.sub.22H.sub.331N.sub.2O.sub.2
(175) NMR .sup.1H (CDCl.sub.3): 0.85 (m, 2H, 2 CH), 1.20 (m, 6H, 6 CH), 1.47 (m, 1H, CH), 1.69 (m, 11H, 7 CH, 2 CHN, 1 CH.sub.2), 2.07 (d, J=7.1 Hz, 2H, CH.sub.2N), 2.84 (m, 2H, 2 CHN), 2.88 (m, 2H, CH.sub.2), 3.84 (s, 3H, CH.sub.3), 4.49 (s, 2H, NH.sub.2), 6.25 (s, 1H, H.sub.Ar), 8.11 (s, 1H, H.sub.Ar).
(176) IR (KBr, cm.sup.1): 3323.7, 3212.5, 2919.4, 2847.4, 2799.7, 2768.1, 1634.4, 1624.9, 1574.7, 1448.8, 1412.1, 1262.9, 1215.3, 1177.1, 1040.6, 834.2.
Example 16
1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cyclohexylethyl)-4-piperidyl]propan-1-one
(177) Compound 16 is prepared according to the operating method of Example 5 described above, considering the following quantities:
(178) tert-butyl 4-[3-(4-amino-5-chloro-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate: 145 mg (0.37 mmol)
(179) Trifluoroacetic acid: 2 ml
(180) DCM: 2 ml
(181) (iodoethyl)cyclohexane: 140 mg (0.44 mmol)
(182) K.sub.2CO.sub.3: 506 mg (3.66 mmol)
(183) DMF: 5 ml
(184) The expected compound is obtained with a yield of 40% and is in the form of a yellow powder.
(185) It has the following characteristics:
(186) MP=170 C.
(187) C.sub.23H.sub.35ClN.sub.2O.sub.2
(188) NMR .sup.1H (CDCl.sub.3): 0.90 (m, 2H, 2 CH), 1.20 (m, 7H, 7 CH), 1.36 (m, 2H, CH.sub.2), 1.61 (m, 9H, 7 CH, CH.sub.2), 1.83 (m, 2H, 2 CHN), 2.28 (m, 2H, CH.sub.2N), 2.89 (m, 4H, 2 CHN, CH.sub.2), 3.82 (s, 3H, CH.sub.3), 4.42 (s, 2H, NH.sub.2), 6.23 (s, 1H, H.sub.Ar), 7.76 (s, 1H, H.sub.Ar).
(189) IR (KBr, cm.sup.1): 3480.5, 2921.3, 2851.8, 1639.6, 1624.0, 1575.6, 1453.0, 1420.3, 1383.6, 1306.2, 1256.9, 1213.7, 1174.9, 1022.2, 830.2.
Example 17
4-amino-5-chloro-[[1-(cyclohexylmethyl)-4-piperidyl]methyl]-2-methoxybenzamide
(190) Compound 17 is prepared according to the operating method of Example 5 described above, considering the following quantities:
(191) tert-butyl 4-[[(4-amino-5-chloro-2-methoxy-benzoyl)amino]methyl]piperidine-1-carboxylate:.sup.vi 66 mg (0.17 mmol)
(192) Trifluoroacetic acid: 1 ml
(193) DCM: 2 ml
(194) (bromomethyl)cyclohexane: 39 mg (0.22 mmol)
(195) K.sub.2CO.sub.3: 232 mg (1.68 mmol)
(196) DMF: 3 ml
(197) The expected compound is obtained with a yield of 38% and is in the form of a yellow solid.
(198) It has the following characteristics:
(199) MP=154-155 C.
(200) C.sub.21H.sub.32ClN.sub.3O.sub.2
(201) NMR .sup.1H (CDCl.sub.3): 0.90 (m, 2H, 2 CH), 1.16 (m, 4H, 4 CH), 1.34 (m, 2H, 2 CH), 1.47 (m, 1H, CH), 1.68 (m, 7H, 7 CH), 1.88 (m, 2H, 2 CHN), 2.11 (d, J=7.1 Hz, 2H, CH.sub.2N), 2.89 (m, 2H, 2 CHN), 3.29 (m, 2H, CH.sub.2N), 3.88 (s, 3H, CH.sub.3), 4.35 (s, 2H, NH.sub.2), 6.27 (s, 1H, H.sub.Ar), 7.73 (t, J=5.2 Hz, 1H, NH), 8.03 (s, 1H, H.sub.Ar).
(202) IR (KBr, cm.sup.1): 3399.9, 2925.1, 2853.7, 1621.7, 1593.0, 1536.1, 1497.9, 1453.4, 1418.5, 1313.7, 1256.5, 1212.0, 1145.4, 989.8, 729.5.
Example 18
4-amino-5-bromo-N-[[1-(cyclohexylmethyl)-4-piperidyl]methyl]-2-methoxybenzamide
(203) Compound 18 is prepared according to the operating method of Example 1 described above, considering the following quantities:
(204) tert-butyl 4-[[(4-amino-5-bromo-2-methoxy-benzoyl)amino]methyl]piperidine-1-carboxylate: 250 mg (0.56 mmol)
(205) Trifluoroacetic acid: 4 ml
(206) DCM: 4 ml
(207) (bromomethyl)cyclohexane: 68 L (0.72 mmol)
(208) K.sub.2CO.sub.3: 92 mg (0.66 mmol)
(209) DMF: 6 ml
(210) MP=162 C.
(211) C.sub.21H.sub.32BrN.sub.3O.sub.2
(212) NMR .sup.1H (CD.sub.3OD): 0.93 (m, 2H), 1.23 (m, 5H), 1.41 (m, 2H), 1.61 (m, 1H), 1.70 (m, 8H), 2.21 (m, 2H), 2.36 (m, 2H), 3.09 (m, 2H), 3.90 (s, 3H, CH.sub.3), 6.27 (s, 1H, H.sub.Ar), 7.94 (s, 1H, H.sub.Ar).
(213) IR (KBr, cm.sup.1): 3450.5, 3406.0, 3319.5, 3195.6, 2918.3, 2847.5, 2763.4, 2417.3, 1634.8, 1593.8, 1543.1, 1496.2, 1463.1, 1448.0, 1309.8, 1255.6, 1210.2, 1179.1, 1134.8, 1041.2, 979.1, 838.9.
Example 19
4-amino-5-iodo-N-[[1-(cyclohexylmethyl)-4-piperidyl]methyl]-2-methoxybenzamide
(214) Compound 19 is prepared according to the operating method of Example 5 described above, considering the following quantities:
(215) tert-butyl 4-[[(4-amino-5-iodo-2-methoxy-benzoyl)amino]methyl]piperidine-1-carboxylate: 138 mg (0.28 mmol)
(216) Trifluoroacetic acid: 1 ml
(217) DCM: 2 ml
(218) (bromomethyl)cyclohexane: 60 mg (0.34 mmol)
(219) K.sub.2CO.sub.3: 390 mg (2.82 mmol)
(220) DMF: 3 ml
(221) The expected compound is obtained with a yield of 40% and is in the form of a yellow powder.
(222) It has the following characteristics:
(223) MP=174 C.
(224) C.sub.21H.sub.32IN.sub.3O.sub.2
(225) NMR .sup.1H (CDCl.sub.3): 0.85 (m, 2H, 2 CH), 1.20 (m, 4H, 4 CH), 1.34 (m, 2H, 2 CH), 1.47 (m, 1H, CH), 1.67 (m, 7H, 7 CH), 1.86 (m, 2H, 2 CHN), 2.10 (d, J=7.1 Hz, 2H, CH.sub.2N), 2.88 (m, 2H, 2 CHN), 3.31 (m, 2H, CH.sub.2N), 3.90 (s, 3H, CH.sub.3), 4.40 (s, 2H, NH.sub.2), 6.27 (s, 1H, H.sub.Ar), 7.69 (t, J=4.8 Hz, 1H, NH), 8.45 (s, 1H, H.sub.Ar).
(226) IR (KBr, cm.sup.1): 3451.5, 3407.6, 3313.5, 3196.4, 2916.7, 2845.4, 2792.0, 1636.2, 1582.8, 1551.4, 1490.2, 1446.1, 1408.6, 1302.1, 1267.0, 1211.3, 1182.3, 1143.0, 1043.1, 977.6, 836.4.
Example 20
[1-(cyclohexylmethyl)-4-piperidyl]methyl 4-amino-5-chloro-2-methoxybenzoate
(227) Under N.sub.2, 89 mg of CDI (0.55 mmol) are added to a suspension of 101 mg of 4-amino-5-chloro-2-methoxybenzoic acid (0.50 mmol) in 3 ml of freshly distilled THF. The reaction medium is stirred at ambient temperature for 24 h then a solution of 106 mg of (1-(cyclohexylmethyl)piperidin-4-yl)methanol (0.50 mmol) in 2 ml of freshly distilled THF then 21 mg of NaH (0.55 mmol). After stirring at AT over a weekend, the THF is evaporated. The residue obtained is taken up in AcOEt, washed in water then dried over MgSO.sub.4. After concentration, the reaction crude is purified over a silica gel column (elution gradient: 100% DCM to 100% AcOEt) to obtain the expected product with a yield of 31%.
(228) Appearance: creamy white solid
(229) MP=129 C.
(230) C.sub.21H.sub.31C1N.sub.2O.sub.3
(231) NMR .sup.1H (CDCl.sub.3): 0.86 (m, 2H, 2 CH), 1.19 (m, 4H, 4 CH), 1.39 (m, 2H, 2 CH), 1.48 (m, 1H, CH), 1.69 (m, 7H, 7 CH), 1.86 (m, 2H, 2 CHN), 2.10 (d, J=7.1 Hz, 2H, CH.sub.2N), 2.88 (m, 2H, 2 CHN), 3.84 (s, 3H, CH.sub.3), 4.08 (d, J=6.0 Hz, 2H, CH.sub.2O), 4.45 (s, 2H, NH.sub.2), 6.29 (s, 1H, H.sub.Ar), 7.81 (s, 1H, H.sub.Ar).
(232) IR (KBr, cm.sup.1): 3472.9, 3329.8, 2919.7, 2850.5, 1694.9, 1621.3, 1600.1, 1449.3, 1316.9, 1275.6, 1234.3, 1109.2, 1072.4, 1053.3, 983.4.
Example 21
1-(4-amino-5-chloro-2-hydroxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one
(233) 72 mg of AlCl.sub.3 (0.54 mmol) and 81 mg of NaI (0.54 mmol) are added to a solution of 140 mg of 1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one (Example 6, 0.36 mmol) in 10 ml of CH.sub.3CN. The reaction medium is then heated at reflux for 4 h. After dilution with DCM, the organic phase is washed in water then saturated NaHCO.sub.3. After drying over MgSO.sub.4, filtration and concentration, the residue is purified over silica gel (elution gradient: DCM to DCM/MeOH (9/1)) to obtain 109 mg of 1-(4-amino-5-chloro-2-hydroxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one with a yield of 81%.
(234) Appearance: pale yellow solid
(235) MP=124 C.
(236) C.sub.21H.sub.31ClN.sub.2O.sub.2
(237) NMR .sup.1H (CDCl.sub.3): 0.86 (m, 2H, 2 CH), 1.22 (m, 6H, 6 CH), 1.48 (m, 1H, CH), 1.71 (m, 9H, 7 CH, CH.sub.2), 1.85 (m, 2H, 2 CHN), 2.10 (d, J=6.8 Hz, 2H, CH.sub.2N), 2.85 (m, 4H, CH.sub.2, 2 CHN), 4.65 (s, 2H, NH.sub.2), 6.23 (s, 1H, H.sub.Ar), 7.61 (s, 1H, H.sub.Ar), 12.72 (s, 1H, OH).
(238) IR (KBr, cm.sup.1): 3471.8, 3368.2, 2920.9, 2849.0, 2800.3, 2766.4, 1636.8, 1521.4, 1505.8, 1448.7, 1424.0, 1379.3, 1352.3, 1320.5, 1270.7, 1230.6, 1212.4, 1112.0, 812.5.
Example 22
1-(4-amino-5-chloro-2-thoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one
(239) 27 mg of K.sub.2CO.sub.3 (0.20 mmol) and 8.54 of iodoethane (0.11 mmol) are added to a solution of 37 mg of 1-(4-amino-5-chloro-2-hydroxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one (0.10 mmol) in 2 ml of DMF. The reaction medium is stirred for 2 h 30 at 110 C. The solution is then diluted with AcOEt then washed 4 times with a saturated NaCl solution. The organic phase is then dried over MgSO.sub.4, filtered and concentrated. The residue obtained is then purified over silica gel (preparation: DCM+2% Et.sub.3N; elution: DCM (9/1)) to obtain 33 mg of expected product (yield=84%) in the form of a white solid.
(240) MP=147 C.
(241) C.sub.23H.sub.35ClN.sub.2O.sub.2
(242) NMR .sup.1H (CDCl.sub.3): 0.81 (m, 2H, 2 CH), 1.20 (m, 6H, 6 CH), 1.44 (t, J=7.1 Hz, 3H, CH.sub.3), 1.47 (m, 1H, CH), 1.68 (m, 11H, 7 CH, 2 CHN, CH.sub.2), 2.05 (d, 1=6.8 Hz, 2H, CH.sub.2N), 2.82 (m, 2H, 2 CHN), 2.92 (t, J=6.8 Hz, 2H, CH.sub.2), 4.03 (qd, J=7.1 Hz, 2H, CH.sub.2O), 4.39 (s, 2H, NH.sub.2), 6.21 (s, 1H, H.sub.Ar), 7.76 (s, 1H, H.sub.Ar).
(243) IR (KBr, cm.sup.1): 3441.1, 3214.2, 2926.3, 2853.7, 1651.8, 1621.7, 1586.1, 1501.8, 1434.3, 1383.5, 1307.3, 1259.7, 1205.7, 1132.7, 1028.4, 802.5.
Example 23
1-[4-amino-5-chloro-2-(2-fluorothoxy)phenyl]-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one
(244) Compound 23 is prepared according to the operating method of Example 22 described above, considering the following quantities:
(245) 1-(4-amino-5-chloro-2-hydroxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one: 44 mg (0.12 mmol)
(246) 2-fluoroethyl tosylate: 28 mg (0.13 mmol)
(247) K.sub.2CO.sub.3: 32 mg (0.23 mmol)
(248) DMF: 3 ml
(249) The expected compound is obtained with a yield of 86% and is in the form of a white solid.
(250) It has the following characteristics:
(251) MP=144-146 C.
(252) C.sub.23H.sub.34ClFN.sub.2O.sub.2
(253) NMR .sup.1H (CDCl.sub.3): 0.82 (m, 2H, 2 CH), 1.18 (m, 6H, 6 CH), 1.44 (m, 1H, CH), 1.66 (m, 11H, 7 CH, CH.sub.2, 2 CHN), 2.05 (d, J=7.1 Hz, 2H, CH.sub.2N), 2.81 (m, 2H, 2 CHN), 2.94 (m, 2H, CH.sub.2), 4.17 (m, &H, CH), 4.24 (m, 1H, CH), 4.43 (s, 2H, NH.sub.2), 4.69 (m, 1H, CH), 4.82 (m, 1H, CH), 6.20 (s, 1H, H.sub.Ar), 7.78 (s, 1H, H.sub.Ar).
(254) IR (KBr, cm.sup.1): 3431.6, 3266.5, 2926.7, 2853.7, 2675.9, 1644.3, 1618.5, 1586.8, 1501.0, 1450.2, 1434.3, 1383.5, 1342.3, 1310.5, 1256.5, 1205.7, 1183.5, 1164.4, 1072.4, 1047.3, 1018.4, 812.0.
Example 24
2-chloro-4-[[2-[1-(cyclohexylmethyl)-4-piperidyl]ethyl]-N-methoxycarbonimidoyl]-5-methoxyaniline
(255) 69 mg of methoxyamine hydrochloride (0.82 mmol) are added to a solution of 90 mg of 1-(4-amino-5-chloro-2-methoxyphenyl)-3-[1-(cyclohexylmethyl)-4-piperidyl]propan-1-one (0.23 mmol) in 2 ml of pyridine. The reaction medium is stirred at ambient temperature for 24 h.
(256) After dilution in water, the aqueous phase is extracted 3 times with AcOEt. The organic phases are combined then washed 5 times in brine, dried over MgSO.sub.4 then concentrated. The residue is purified over silica gel (preparation: DCM+2% Et.sub.3N; elution gradient DCM/AcOEt: 10/0 to 9/1) to obtain 41 mg of expected product (stereoisomer E) in the form of a pale yellow oil (yield=42%).
(257) C.sub.23H.sub.36C1N.sub.3O.sub.2
(258) NMR .sup.1H (CDCl.sub.3): 0.83 (m, 2H, 2 CH), 1.22 (m, 8H, 6 CH, CH.sub.2), 1.44 (m, 1H, CH), 1.65 (m, 9H, 7 CH, 2 CHN), 2.04 (d, J=6.8 Hz, 2H, CH.sub.2N), 2.63 (m, 2H, CH.sub.2), 2.79 (m, 2H, 2 CHN), 3.72 (s, 3H, CH.sub.3), 3.89 (s, 3H, CH.sub.3), 4.09 (s, 2H, NH.sub.2), 6.25 (s, 1H, H.sub.Ar), 7.12 (s, 1H, H.sub.Ar).
(259) IR (KBr, cm.sup.1): 3475.2, 3385.4, 2921.3, 2849.7, 2800.7, 2766.8, 1621.2, 1506.2, 1463.8, 1450.4, 1411.0, 1337.9, 1257.8, 1212.9, 1176.3, 1051.6, 984.8, 877.6.
Example 25vii
Ethyl 3-(2-methoxy-4-methylphenyl)-3-oxopropanoate
(260) 320 mg of CDI (1.98 mmol) are carefully added to a suspension of 300 mg of 4-amino-2-methoxybenzoic (1.78 mmol) acid in 15 ml of distilled THF. The mixture is stirred at ambient temperature for 4 h. 336 mg of 3-ethoxy-3-oxopropanoate potassium salt (2.16 mmol) and 205 mg of MgCl.sub.2 (2.16 mmol) are then added per portion. The reaction mixture is stirred at ambient temperature for 2 days. After extending with 30 ml of Et.sub.2O, the solution is then washed with water, a saturated solution of NaHCO.sub.3 and a saturated solution of NaCl. After drying over MgSO.sub.4, the organic phase is evaporated and the crude product is purified over silica gel (elution gradient: DCM to DCM/AcOEt 95/5) to give 194 mg of ethyl 3-(4-amino-2-methoxyphenyl)-3-oxopropanoate with a yield of 46%.
(261) Appearance: colorless oil
(262) C.sub.12H.sub.15NO.sub.4
(263) NMR .sup.1H (CDCl.sub.3): 1.24 (t, J=7.8 Hz, 3H, CH.sub.3), 3.82 (s, 3H, CH.sub.3), 3.88 (s, 2H, CH.sub.2), 4.17 (q, J=7.8 Hz, 2H, CH.sub.2), 4.18 (s, 2H, NH.sub.2), 6.12 (d, J=2.1 Hz, 1H, H.sub.Ar), 6.27 (dd, J=8.8 Hz, J=2.1 Hz, 1H, H.sub.Ar), 7.83 (d, J=8.8 Hz, 1H, H.sub.Ar).
(264) IR (KBr, cm.sup.1): 3460.2, 3365.2, 3228.0, 2980.7, 1729.3, 1640.7, 1592.1, 1471.0, 1433.6, 1322.6, 1253.2, 1215.2, 1129.9, 1026.2, 830.3.
Example 2610
Ethyl 3-(4-amino-5-chloro-2-methoxy-phenyl)-3-oxopropanoate
(265) 533 mg of CDI (3.29 mmol) are carefully added to a suspension of 603 mg of 4-amino-5-chloro-2-methoxybenzoic acid (2.99 mmol) in 30 ml of distilled THF..sup.10 The mixture is stirred at ambient temperature for 6 h. Then, 611 mg of 3-ethoxy-3-oxopropanoate potassium salt (3.59 mmol) and 342 mg of MgCl.sub.2 (3.59 mmol) are added per portion. The reaction mixture is stirred at 40 C. for 2 days. After extending with 30 ml of Et.sub.2O, the solution is then washed with water, a saturated solution of NaHCO.sub.3 and a saturated solution of NaCl. After drying over MgSO.sub.4, the organic phase is evaporated and the crude product is purified over silica gel (gradient elution: CH/AcOEt 8/2 to 7/3) to give 507 mg of ethyl 3-(4-amino-5-chloro-2-methoxyphenyl)-3-oxopropanoate with a yield of 62%.
(266) Appearance: white solid
(267) MP=124 C.
(268) C.sub.12H.sub.14ClNO.sub.4
(269) NMR .sup.1H (CDCl.sub.3): 1.24 (t, J=7.3 Hz, 3H, CH.sub.3), 3.82 (s, 3H, CH.sub.3), 3.87 (s, 2H, CH.sub.2), 4.17 (q, J=7.3 Hz, 2H, CH.sub.2), 4.56 (s, 2H, NH.sub.2), 6.23 (s, 1H, H.sub.Ar), 7.92 (s, 1H, H.sub.Ar).
(270) IR (KBr, cm.sup.1): 3463.0, 3362.5, 3222.9, 2982.6, 1725.0, 1647.9, 1621.7, 1572.8, 1468.9, 1456.6, 1422.8, 1325.5, 1261.6, 1221.0, 1155.4, 1024.0, 836.8.
Example 27
Ethyl 3-(4-amino-5-fluoro-2-methoxy-phenyl)-3-oxopropanoate
(271) Compound 27 is prepared according to the operating method of Example 26 described above, considering the following quantities:
(272) 4-amino-5-fluoro-2-methoxybenzoic acid: 272 mg (1.47 mmol)
(273) CDI: 262 mg (1.62 mmol)
(274) 3-ethoxy-3-oxopropanoate potassium salt: 300 mg (1.76 mmol)
(275) MgCl.sub.2: 168 mg (1.76 mmol)
(276) THF: 30 ml
(277) The expected compound is obtained with a yield of 65% and is in the form of a white solid.
(278) MP=83-85 C.
(279) C.sub.12H.sub.14FNO.sub.4
(280) NMR .sup.1H (CDCl.sub.3): 1.24 (t, J=7.1 Hz, 3H, CH.sub.3), 3.82 (s, 3H, CH.sub.3), 3.88 (s, 2H, CH.sub.2), 4.17 (q, J=7.1 Hz, 2H, CH.sub.2), 4.26 (s, 2H, NH.sub.2), 6.24 (d, J=7.1 Hz, 1H, H.sub.Ar), 7.65 (d, J=11.9 Hz, 1H, H.sub.Ar).
(281) IR (KBr, cm.sup.1): 3469.7, 3464.2, 3231.6, 2981.7, 1729.6, 1652.9, 1628.0, 1604.2, 1522.4, 1469.6, 1431.7, 1366.9, 1319.2, 1250.2, 1211.3, 1189.8, 1139.2, 1026.3, 829.9.
Example 28
Ethyl 3-(4-amino-5-bromo-2-methoxy-phenyl)-3-oxopropanoate
(282) Compound 28 is prepared according to the operating method of Example 26 described above, considering the following quantities:
(283) 4-amino-5-bromo-2-methoxybenzoic acid: 500 mg (2.03 mmol)
(284) CDI: 362 mg (2.23 mmol)
(285) 3-ethoxy-3-oxopropanoate potassium salt: 414 mg (2.44 mmol)
(286) MgCl.sub.2: 232 mg (2.44 mmol)
(287) THF: 30 ml
(288) The expected compound is obtained with a yield of 50% and is in the form of a white powder.
(289) It has the following characteristics:
(290) MP=128 C.
(291) C.sub.12H.sub.14BrNO.sub.4
(292) NMR .sup.1H (CDCl.sub.3): 1.24 (t, J=7.8 Hz, 3H, CH.sub.3), 3.83 (s, 3H, CH.sub.3), 3.87 (s, 2H, CH.sub.2), 4.18 (q, J=7.8 Hz, 2H, CH.sub.2), 4.59 (s, 2H, NH.sub.2), 6.23 (s, 1H, H.sub.Ar), 8.08 (s, 1H, H.sub.Ar).
(293) IR (KBr, cm.sup.1): 3456.3, 3358.0, 3217.6, 2981.0, 1724.9, 1647.4, 1620.4, 1578.0, 1468.2, 1454.3, 1420.4, 1321.5, 1265.7, 1221.2, 1153.7, 1023.7, 835.0.
Example 29
Ethyl 3-(4-amino-5-iodo-2-methoxy-phenyl)-3-oxopropanoate
(294) Compound 29 is prepared according to the operating method of Example 26 described above, considering the following quantities:
(295) 4-amino-5-iodo-2-methoxybenzoic acid: 355 mg (1.21 mmol)
(296) CDI: 216 mg (1.33 mmol)
(297) 3-ethoxy-3-oxopropanoate potassium salt: 247 mg (1.45 mmol)
(298) MgCl.sub.2: 138 mg (1.45 mmol)
(299) THF: 30 ml
(300) The expected compound is obtained with a yield of 50% and is in the form of a white powder.
(301) It has the following characteristics:
(302) MP=140 C.
(303) C.sub.12H.sub.14INO.sub.4
(304) NMR .sup.1H (CDCl.sub.3): 1.24 (t, J=7.8 Hz, 3H, CH.sub.3), 3.82 (s, 3H, CH.sub.3), 3.87 (s, 2H, CH.sub.2), 4.17 (q, J=7.8 Hz, 2H, CH.sub.2), 4.65 (s, 2H, NH.sub.2), 6.22 (s, 1H, H.sub.Ar), 8.25 (s, 1H, H.sub.Ar).
(305) IR (KBr, cm.sup.1): 3454.0, 3350.0, 3212.1, 2976.2, 1732.2, 1645.6, 1619.0, 1569.6, 1467.0, 1454.5, 1421.4, 1325.2, 1264.7, 1225.9, 1190.5, 1153.4, 1031.9, 831.2.
Example 30viii
Tert-butyl 4-[3-(4-amino-2-methoxyphenyl)-3-oxopropyl]piperidine-1-carboxylate
(306) 437 mg of K.sub.2CO.sub.3 (3.16 mmol) and 617 mg of tert-butyl 4-(iodomethyl)piperidine-1-carboxylate (1.89 mmol) are added to a solution of 379 mg of the compound of Example 22 (1.58 mmol) in 30 ml of DMF. The reaction mixture is stirred at ambient temperature for 1 week. After dilution in water, the product is extracted with Et.sub.2O (340 ml). The organic phases are combined washed 3 times with water and once with brine then dried over MgSO.sub.4. After concentration, the residue is dissolved in 10 ml of a binary mixture EtOH/H.sub.2O (5/1) to which 407 mg of KOH (7.27 mmol) are added. The new reaction medium is then heated at reflux for 4 hours. The EtOH is removed in a rotary evaporator then the resulting aqueous phase is diluted with water, extracted with DCM. The organic phases are combined, washed with water and brine, dried over MgSO.sub.4 then concentrated. The crude product is purified over silica gel (elution gradient: DCM to DCM/AcOEt 6/4) to obtain 469 mg of tert-butyl 4-[3-(4-amino-2-methoxy)-3-oxopropyl]piperidine-1-carboxylate with a yield of 82%.
(307) Appearance: yellow oil
(308) C.sub.20H.sub.30N.sub.2O.sub.4
(309) NMR .sup.1H (CDCl.sub.3): 1.11 (m, 2H, 2 CH), 1.45 (m, 10H, 3 CH.sub.3, CH), 1.61 (m, 2H, CH.sub.2), 1.68 (m, 2H, 2 CH), 2.67 (m, 2H, 2 CHN), 2.90 (m, 2H, CH.sub.2), 3.86 (s, 3H, CH.sub.3), 4.07 (m, 2H, 2 CHN), 4.62 (s, 2H, NH.sub.2), 6.16 (d, J=2.0 Hz, 1H, H.sub.Ar), 6.27 (dd, J=8.6 Hz, J=2.0 Hz, 1H, H.sub.Ar), 7.71 (d, J=8.6 Hz, 1H, H.sub.Ar).
(310) IR (KBr, cm.sup.1): 3442.8, 3355.8, 3240.2, 2974.2, 2930.5, 2851.3, 1675.9, 1642.3, 1595.9, 1468.4, 1431.1, 1365.6, 1277.7, 1212.9, 1162.6.
Example 31
Tert-butyl 4-[3-(4-amino-5-chloro-2-methoxyphenyl)-2-methyl-3-oxopropyl]piperidine-1-carboxylate
(311) Under N.sub.2, at 10 C., 909 L of LIHMDS 1M in THF (0.909 mmol) are added to a solution of 300 mg of tert-butyl 4-[3-(4-amino-5-chloro-2-methoxyphenyl)-2-methyl-3-oxopropyl]piperidine-1-carboxylate (0.76 mmol) in 3 ml of freshly distilled THF. The reaction medium is stirred for 15 minutes at 10 C. then 57 ml of iodomethane (0.909 mmol) are added. After 3 h and a slow return to AT, the solution is concentrated under reduced pressure. The residue is taken up in AcOEt and washed twice with water. The organic phase is dried over MgSO.sub.4 then concentrated. The crude product is purified over silica gel (elution gradient: DCM to DCM/AcOEt 95/5) to obtain 76 mg of expected product (yield=25%).
(312) Appearance: pale yellow oil
(313) C.sub.21H.sub.31ClN.sub.2O.sub.4
(314) NMR .sup.1H (CDCl.sub.3): 1.00 (m, 2H, 2 CH), 1.06 (d, J=7.1 Hz, 3H, CH.sub.3) 1.18 (m, 1H, 1 CH), 1.46 (m, 10H, 3 CH.sub.3, CH), 1.55 (m, 2H, 2 CH), 1.68 (m, 1H, 1 CH), 2.62 (m, 2H, 2 CHN), 2.90 (m, 2H, 2 CHN), 3.58 (sext, J=7.1 Hz, 1H, CH), 3.81 (s, 3H, CH.sub.3), 4.01 (m, 2H, 2 CHN), 4.44 (s, 2H, NH.sub.2), 6.24 (s, 1H, H.sub.Ar), 7.67 (s, 1H, H.sub.Ar).
(315) IR (KBr, cm.sup.1): 3471.8, 3350.1, 3240.2, 2973.9, 2927.8, 2849.4, 1673.2, 1622.4, 1590.1, 1465.1, 1419.2, 1365.5, 1278.3, 1250.5, 1215.6, 1174.8.
Example 32
Tert-butyl 4-[3-(4-amino-5-fluoro-2-methoxyphenyl)-3-oxopropyl]piperidine-1-carboxylate
(316) Compound 32 is prepared according to the operating method of Example 30 described above, considering the following quantities:
(317) Ethyl 3-(4-amino-5-fluoro-2-methoxyphenyl)-3-oxopropanoate: 185 mg (0.72 mmol)
(318) Tert-butyl 4-(iodomethyl)piperidine-1-carboxylate: 283 mg (0.87 mmol)
(319) K.sub.2CO.sub.3: 200 mg (1.45 mmol)
(320) DMF: 5 ml
(321) KOH: 187 mg (3.33 mmol)
(322) EtOH: 10 ml
(323) H.sub.2O: 2 ml
(324) The expected compound is obtained with a yield of 70% and is in the form of a white powder.
(325) It has the following characteristics:
(326) MP=171 C.
(327) C.sub.20H.sub.29FN.sub.2O.sub.4
(328) NMR .sup.1H (CDCl.sub.3): 1.11 (m, 2H, 2 CH), 1.43 (m, 1H, CH), 1.45 (s, 9H, 3 CH.sub.3), 1.59 (m, 2H, CH.sub.2), 1.67 (m, 2H, 2 CH), 2.67 (m, 2H, 2 CH), 2.92 (m, 2H, CH.sub.2), 3.84 (s, 3H, CH.sub.3), 4.08 (m, 2H, 2 CHN), 4.15 (s, 2H, NH.sub.2), 6.27 (d, J.sub.ii-F=7.1 Hz, 1H, H.sub.Ar), 7.94 (s, J.sub.H-F=11.9 Hz, 1H, H.sub.Ar).
(329) IR (KBr, cm.sup.1): 3438.0, 3350.6, 3229.9, 2974.2, 2927.4, 2853.0, 1675.1, 1628.6, 1604.4, 1467.6, 1428.0, 1365.6, 1311.5, 1249.4, 1168.8.
Example 33
Tert-butyl 4-[3-(4-amino-5-bromo-2-methoxyphenyl)-3-oxopropyl]piperidine-1-carboxylate
(330) Compound 33 is prepared according to the operating method of Example 30 described above, considering the following quantities:
(331) Ethyl 3-(4-amino-5-bromo-2-methoxyphenyl)-3-oxopropanoate: 250 mg (0.79 mmol)
(332) tert-butyl 4-(iodomethyl)piperidine-1-carboxylate: 309 mg (0.95 mmol)
(333) K.sub.2CO.sub.3: 218 mg (158 mmol)
(334) DMF: 5 ml
(335) KOH: 204 mg (3.63 mmol)
(336) EtOH: 8 ml
(337) H.sub.2O: 2 ml
(338) The expected compound is obtained with a yield of 68% and is in the form of a white powder.
(339) It has the following characteristics:
(340) MP=146-148 C.
(341) C.sub.20H.sub.29BrN.sub.2O.sub.4
(342) NMR .sup.1H (CDCl.sub.3): 1.07 (m, 2H, 2 CH), 1.43 (m, 10H, 3 CH.sub.3, CH), 1.57 (m, 2H, CH.sub.2), 1.64 (m, 2H, 2 CH), 2.64 (m, 2H, 2 CH), 2.88 (m, 2H, CH.sub.2), 3.81 (s, 3H, CH.sub.3), 4.06 (m, 2H, 2 CHN), 4.61 (s, 2H, NH.sub.2), 6.25 (s, 1H, H.sub.Ar), 7.91 (s, 1H, H.sub.Ar).
(343) IR (KBr, cm.sup.1): 3469.7, 3353.1, 3225.2, 2977.5, 2928.4, 2853.7, 1688.8, 1672.5, 1621.6, 1584.9, 1419.6, 1365.8, 1311.6, 1250.4, 1218.0, 1172.8.
Example 34
Tert-butyl 4-[3-(4-amino-5-iodo-2-methoxyphenyl)-3-oxopropyl]piperidine-1-carboxylate
(344) Compound 33 is prepared according to the operating method of Example 30 described above, considering the following quantities:
(345) Ethyl 3-(4-amino-5-iodo-2-methoxyphenyl)-3-oxopropanoate: 172 mg (0.47 mmol)
(346) Tert-butyl 4-(iodomethyl)piperidine-1-carboxylate: 169 mg (0.52 mmol)
(347) K.sub.2CO.sub.3: 131 mg (0.95 mmol)
(348) DMF: 5 ml
(349) KOH: 122 mg (2.18 mmol)
(350) EtOH: 8 ml
(351) H.sub.2O: 2 ml
(352) The expected compound is obtained with a yield of 65% and is in the form of a white powder.
(353) It has the following characteristics:
(354) MP=152 C.
(355) C.sub.20H.sub.29IN.sub.2O.sub.4
(356) NMR .sup.1H (CDCl.sub.3): 1.10 (m, 2H, 2 CH), 1.45 (m, 10H, 3 CH.sub.3, CH), 1.59 (m, 2H, CH.sub.2), 1.67 (m, 2H, 2 CH), 2.67 (m, 2H, 2 CHN), 2.90 (m, 2H, CH.sub.2), 3.85 (s, 3H, CH.sub.3), 4.08 (m, 2H, 2 CHN), 4.51 (s, 2H, NH.sub.2), 6.25 (s, 1H, H.sub.Ar), 8.13 (s, 1H, H.sub.Ar).
(357) IR (KBr, cm.sup.1): 3459.4, 3341.2, 3219.9, 2972.8, 2928.5, 2850.1, 1672.7, 1621.3, 1578.9, 1451.4, 1415.0, 1365.2, 1265.5, 1217.3, 1164.6.
Example 35
Tert-butyl 4-[[(4-amino-5-bromo-2-methoxybenzoyl)amino]methyl]piperidine-1-carboxy-late
(358) 269 ml of Et.sub.3N (1.93 mmol) and 414 mg of tert-butyl 4-(aminomethyl)piperidine-1-carboxylate (1.93 mmol) are added to a solution of 474 mg of 4-amino-5-bromo-2-methoxybenzoic acid (1.93 mmol) in 4 ml of DMF.sup.9. The solution is held at 5 C. then 261 mg de HOBT (1.93 mmol) and 371 mg of EDCI.HCl (1.93 mmol) are added. The reaction medium is then stirred overnight at AT. The solution is diluted with water then extracted 3 times with AcOEt. The organic phases are combined then washed 4 times with water. The organic phase is dried over MgSO.sub.4, filtered then concentrated. The reaction crude is then purified over silica gel (elution gradient: DCM to DCM/AcOEt 7/3) to obtain 630 mg of expected product (yield=74%).
(359) MP=125 C.
(360) C.sub.19H.sub.28BrN.sub.3O.sub.4RMN .sup.1H (CDCl.sub.3): 1.17 (m, 2H, 2 CH), 1.45 (m, 9H, 3 CH.sub.3), 1.75 (m, 3H, 3 CH), 2.69 (m, 2H, 2 CHN), 3.32 (m, 2H, CH.sub.2N), 3.89 (s, 3H, CH.sub.3), 4.10 (m, 2H, 2 CHN), 4.53 (s, 2H, NH.sub.2), 6.32 (s, 1H, H.sub.Ar), 7.76 (t, J=5.6 Hz, 1H, NH), 8.24 (s, 1H, H.sub.Ar).
(361) IR (KBr, cm.sup.1): 3448.2, 3337.2, 3206.8, 2970.9, 2927.7, 2854.9, 1689.0, 1631.6, 1593.5, 1559.2, 1491.2, 1465.4, 1430.2, 1364.5, 1247.0, 1179.2, 1147.3.
Example 36
Tert-butyl 4-[[(4-amino-5-iodo-2-methoxybenzoyl)amino]methyl]piperidine-1-carboxylate
(362) Compound 35 is prepared according to the operating method of Example 34 described above, considering the following quantities:
(363) 4-amino-5-iodo-2-methoxybenzoic acid: 342 mg (1.16 mmol)
(364) Tert-butyl 4-(aminomethyl)piperidine-1-carboxylate: 214 mg (1.16 mmol)
(365) Et.sub.3N: 162 l (1.16 mmol)
(366) EDCI.HCl: 223 mg (1.16 mmol)
(367) HOBT: 157 mg (1.16 mmol)
(368) DMF: 5 ml
(369) The expected compound is obtained with a yield of 80% and is in the form of a pale yellow powder.
(370) It has the following characteristics:
(371) MP=135 C.
(372) C.sub.19H.sub.28IN.sub.3O.sub.4
(373) NMR .sup.1H (CDCl.sub.3): 1.18 (m, 2H, 2 CH), 1.45 (m, 9H, 3 CH.sub.3), 1.73 (m, 3H, 3 CH), 2.69 (m, 2H, 2 CHN), 3.32 (m, 2H, CH.sub.2N), 3.90 (s, 3H, CH.sub.3), 4.12 (m, 2H, 2 CHN), 4.42 (s, 2H, NH.sub.2), 6.28 (s, 1H, H.sub.Ar), 7.72 (t, 1=5.8 Hz, 1H, NH), 8.45 (s, 1H, H.sub.Ar).
(374) IR (KBr, cm.sup.1): 3408.0, 3333.5, 3209.0, 2973.8, 2927.4, 2850.1, 1679.3, 1627.9, 1586.5, 1536.2, 1491.2, 1465.4, 1425.6, 1365.3, 1251.7, 1212.4, 1171.3, 1142.5.
(375) The biological properties of the above-mentioned compounds according to the invention have been tested in order to determine: their affinity as regards the 5-HT4 receptor the power of their agonist effect as regards the 5-HT4 receptor their inhibitory effect of acetylcholinesterase (Ellman test) their interaction with the peripheral site of acetylcholinesterase (propidium displacement test) their capacity to increase in vivo in the rodent the extinction time of the memory trace in an object recognition test modelling the episodic memory (nature of the object) of the rodent (object recognition test).
A) Affinity as Regards 5-HT4 Receptors
Equipment and Methods
(376) Sampling Striatal Tissue and Membrane Preparation
(377) All of the procedures described in this chapter are the result of the work of Grossman et al. (1993). In brief, the animals (Male Guinea pigs: 300-350 g, IFFA CREDO, France) are euthanized by decapitation then the brain is quickly removed at +4 C. The striatal regions are carefully dissected then reassembled. The striata assembly is placed in 10 volumes of 50 mM HEPES buffer, pH 7.4, at +4 C. After homogenisation at +4 C. (Ultra-Turrax, maximum speed, 15 sec) and ultracentrifugation (23 000 g, 60 min, +4 C.), the cell pellet is re-suspended in 50 mM, pH 7.4 buffer at +4 C. so as to obtain a tissue concentration in the order of 15 mg.Math.ml.sup.1 (protein assay by the Lowry et al. method, 1951, using bovine serum albumin as a standard).
(378) Competition Studies of the Products to be Studied in Competition Against [.sup.3H]-GR113808
(379) Prior to these competition studies, a series of saturation curves were performed in order to check whether the pharmacological parameters Kd, Bmax and the Hill Coefficient of [.sup.3H]-GR113808, obtained in our experimental conditions, are consistent with those published in the literature. For that purpose, 7.5 g/L membrane preparation samples are incubated in duplicate (50 mM HEPES buffer, pH 7.4, +37 C.) at +37 C. for 30 minutes, in the presence of 7 increasing concentrations (0.05-1.5 nM, 200 l of final volume) of [3H]-GR113808 (Grossman et al., 1993). Using a Brandel Cell Harvester, the binding of the radioligand with the proteins is interrupted by rapid filtration of the incubation medium through a filter strip (WHATMAN GF/B FP-100) pre-incubated in polyethylenimine (PEI 0.5% in water); filtration is followed by 3 rinses with 4 ml of 50 mM HEPES buffer, pH 7.4, at +4 C. The non-specific binding of [.sup.3H]-GR113808 is quantified in the presence of 30 M of serotonin, specific binding thus being estimated as the difference: (binding in the absence of serotonin)(binding in the presence of serotonin).
(380) The competition studies are carried out with 0.1 nM of [.sup.3H]-GR113808 in the presence of 10.sup.6 or 10.sup.8 M, or n concentrations for the Kis, of ligand to be studied, prucalopride being used as the reference 5-HT4 agonist. After incubation, the filtration and rinsing procedures were identical to those described above.
(381) Results
(382) By way of example, the affinities of several compounds according to the invention as regards 5-HT4 receptors are listed in Table 4.
(383) TABLE-US-00004 TABLE 4 Affinity of several compounds according to the invention as regards 5-HT4 receptors, prucalopride being taken as a reference (Ki in nM). Compound Ki in nM 1 88.6 2 8.9 3 44.7 4 125.0 5 3.8 6 14.8 7 8.8 8 3.0 9 7.1 10 125.0 11 8.0 12 3.9 13 2.5 14 17.0 15 18.1 16 8.2 17 2.3 18 5.4 19 13.9 20 0.9 21 125.0 22 12.5 23 2.5 24 13.1 prucalopride 44.1
B) Agonist Nature as Regards the 5-HT4 Receptor
Equipment and Methods
(384) The agonist nature as regards the 5-HT4 receptor of several compounds according to the invention is determined by the measurement of the accumulation of intracellular cyclic AMP. For this, transfected stable cells are cultivated until confluence with a serum-free medium 4 h before the start of the experiment. The cells are then pre-incubated for 15 minutes in a serum-free medium supplemented with 5 mM of theophylline, 10 M of pargyline and 1 M of GR127935 compound in CHO cells to block the endogenous activity of the 5-HT1B receptors. Serotonin is then added for 15 more minutes. The reaction is stopped by aspiration of the medium and the addition of 500 L of ice-cold ethanol. After 30 min of incubation, the ethanolic fraction is collected and evaporated under vacuum. The pellet is then reconstituted and the cyclic AMP is quantified by radioimmunoassay (cyclic AMP competitive radioimmunoassay, Immunotech, Marseille, France). The Student Tests are performed using QuickTest software. The results are expressed as a percentage of agonist effect in relation to serotonin. Prucalopride, a full 5-HT4 receptor agonist, is used as a reference.
(385) Results
(386) By way of example, the powers of agonist effect of several compounds according to the invention as regards 5-HT4 receptors are shown in Table 5.
(387) TABLE-US-00005 TABLE 5 Power of the agonistic effect of several compounds according to the invention as regards 5-HT4 receptors (%). Compound Agonistic effect % 5 16% 8 12% 9 22% 12 21% prucalopride 100%
C) Ellman Test
Equipment and Methods
(388) Acetylcholinesterase extracted from human erythrocytes (buffered aqueous solution, 500 units/mg, Sigma Aldrich) is diluted in a 20 mM HEPES buffer pH8, 0.1% Triton X-100, to obtain a standard solution with 2.5 units of enzyme activity/ml. 100 L of a 0.3 mM 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) solution in a pH 7.4 phosphate buffer are introduced into a 96-well plate, followed by 50 L of compound to be tested in solution in DMSO and 50 L of enzyme solution. After 5 min of preincubation, the reaction is initiated by injecting 50 L of 10 mM acetylthiocholine iodide solution. Hydrolysis of the acetylthiocholine is followed by the formation of the anion 5-thio-2-nitrobenzoate, produced by the reaction of the DTNB with the thiocholine released by the enzymatic hydrolysis of the acetylthiocholine, by means of a microplate reader (TECAN Infinite M200, Lyon, France) at a wavelength of 412 nm per minute for 10 minutes. Donepezil is used as a reference product.
(389) For compounds showing a significant inhibition of acetylcholinesterase (50%) after 4 min of reaction, the values of IC.sub.50 are measured graphically at six points on the inhibition curve by Origin software.
(390) Results
(391) By way of example, the inhibitory activities of human acetylcholinesterase of several compounds according to the invention as regards 5-HT4 receptors are show in Table 6.
(392) TABLE-US-00006 TABLE 6 Inhibition of human acetylcholinesterase of several compounds according to the invention, Donepezil being used as a reference (IC.sub.50 in nM). Compound IC.sub.50 in nM 1 748 2 445 3 658 4 26 5 24 6 937 7 577 8 69 9 63 10 201 11 321 12 57 13 118 14 99 15 304 16 222 17 3730 18 8700 19 5090 20 2720 21 411 22 395 23 625 24 320 Donepezil 11
D) Propidium Displacement Test
Equipment and Methods
(393) When propidium diiodide binds to the peripheral site of AChE, it produces an increase in fluorescence which can be used as proof of its binding to the enzyme. Fluorescence measurements are made in 200 L of solution in 96-well plates using a Tecan Infinite M2000 microplate reader. Five units of eeAChE are incubated for 15 min at 25 C. in a 1 mM Tris-HCl, pH 8.0, buffer with 150 L of 10.sup.5M solution of compounds to be tested or Donepezil as a reference. 50 L of micromolar solution of propidium diiodide are added before measuring fluorescence. The excitation wavelength is measured at 535 nm, and the emission wavelength at 595 nm. Each test is repeated at least three times.
(394) Results
(395) By way of example, the propidium diiodide displacement values of the peripheral site of eeAChE of several compounds according to the invention are shown in Table 7.
(396) TABLE-US-00007 TABLE 7 Propidium diiodide displacement from the peripheral site of eeAChE of several compounds according to the invention (%) % Propidium Compound Displacement 5 22% 8 20% 9 22% 12 20% 14 20% 16 21% 23 22% Donepezil 23%
E) Object Recognition Test
Equipment and Methods
(397) Animals
(398) The tests are performed using male NMRI mice of around 3 months old at the time of the tests. These mice are kept in groups of 15 in standard polycarbonate cages (422915 cm.sup.3), in an animal housing facility kept at a temperature of 222 C. and relative humidity of 5510%. They have free access to food and water and are kept in a reverse cycle (light phase between 20.00 and 8.00) to enable experiments to be performed during their phase of activity.
(399) Object Recognition Test
(400) The object recognition test is based on the spontaneous preference of rodents for novelty, without bringing food deprivation or electric shock into play. This test allows a form of episodic memory in the rodent to be assessed in the sense that the ability of the animal to store information connected with a spatio-temporal context is assessed (Dere et al., 2007).
(401) The device comprises a square PVC enclosure (333320 cm, illuminated at 10 lux in the center) with black surfaces. The test is performed in 2 phases: one habituation phase, allowing the animals to become habituated to the test environment (enclosure, room, experimenter), the other a memory performance assessment phase. A camera is positioned above the device and connected to a video-tracking system (ViewPoint). During the behavioral tests, the experimenter is not present in the experimentation room but remains in an adjacent room that houses the video-tracking system.
(402) After a habituation phase (Day 1, individual exposure for 5 minutes to the open-field with no objects), the memory performance assessment phase begins on the 2nd day and is made up of 2 sessions, one the presentation session and the other the test session. Immediately before the presentation session, the mouse is habituated again with the equipment for one minute, then two identical objects, A1 and A2, are placed at about 5 cm from the walls of the enclosure. The mouse is placed with its back to the objects, facing the experimenter, and freely explores the device. The session is stopped when the mouse reaches a total exploration time of the two objects of 30 seconds. The exploration time of each object, A1 and A2 respectively, as well as the time required to achieve the exploration criterion are measured. After an intersession period of 24 hours, a test session is performed. Two objects are used: one familiar object A3 (a copy of A1 or A2 not previously presented) and one object B, different from object A, which will constitute the new object. The session is stopped by the experimenter when the mouse achieves a total exploration criterion of 30 seconds. The time required to achieve the exploration criterion as well as the exploration time of the new object are measured and the latter is compared to the chance level (15 s).
(403) The objects used on the test day are a blue ceramic dolphin (4 cm in diameter and 10 cm high) and a transparent bottle filled with sand (34.59 cm), available in four copies, which are cleaned after each use (70% ethanol). These objects are glued to the floor of the open field with Patafix so that the mice cannot move them. The combinations of objects (bottle/bottle and dolphin/dolphin) and the placing of a new object (left/right) are determined at random to avoid bias due to a possible preference for an object or place.
(404) The results are expressed by the exploration percentage of each object during the test session. The data are analyzed by an analysis of variance (ANOVA) with repeated measurements, followed by a Student-Newman-Keuls (SNK) multiple comparison test.
(405) Administration of the Compounds
(406) Batches of 10 to 12 mice are formed into an experimental group. The compounds in solution in the physiological serum are administered intraperitoneally (volume of injection, 10 ml/kg of body weight), 30 minutes before the acquisition session. A control batch (physiological serum) is created.
(407) Results (
(408) By way of example, Compound 9 results in an improvement in object recognition performance compared to the control batch with a maximum effect after the dose of 0.3 mg/kg