Reaction casing for a photosynthetic reactor and associated photosynthetic reactor
09663749 ยท 2017-05-30
Assignee
Inventors
Cpc classification
Y10T29/49826
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
International classification
Abstract
Reaction casing (1) for a photosynthetic reactor suitable for cultivating photosynthetic microorganisms, in particular algae, said reaction casing (1) being designed for both floating on an expanse of water and defining a path for a gas/liquid culture medium to flow in two phases between a first and a second opening (11, 12) of said casing. The casing comprises, on the one hand, an upper membrane (31) and a lower membrane (32) made at least partially of a flexible material, hermetically sealed and transparent to light radiation, said membranes being hermetically connected to one another by connecting lines (41, 42) defining inflatable cells (33, 34) and, on the other hand, connection elbows (35, 36) joining said cells in pairs to define the course of flow in a generally sinuous form, one of the cells (33) being fluidly linked to the first opening and another cell (34) being fluidly linked to the second opening (12). The present invention is applicable in the field of photosynthetic microorganism cultivation, in particular algae cultivation.
Claims
1. A reaction casing for a photosynthetic reactor suitable for cultivating photosynthetic microorganisms said reaction casing being designed to float on an expanse of water and to delimit a two-phase flow pathway for a gas/liquid culture medium between a first and second opening of said reaction casing, said reaction casing comprising, an upper membrane and a lower membrane made at least partially from a material that is flexible, watertight and transparent to light radiation, said membranes being hermetically connected along junction lines delimiting adjacent inflatable cells and, on the other hand, junction elbows pairwise joining said cells to define said flow path of generally sinuous shape, one of the cells being in fluid connection with the first opening and another cell being in fluid connection with the second opening, wherein the junction elbows are inflatable junction elbows and are made at least partially from a flexible and watertight material, wherein the casing is composed of three distinct portions comprising an upstream portion, a downstream portion and a central portion interposed between the upstream and downstream portions, wherein: the upstream portion is composed of an assembly of an upper skin and a lower skin hermetically connected to each other along junction lines in order to delimit both upstream portions of the cells and upstream inflatable junction elbows, the two openings being provided in this upstream portion; the downstream portion is composed of an assembly of an upper skin and a lower skin hermetically connected to each other along junction lines in order to delimit both downstream portions of the cells and downstream inflatable junction elbows; and the central portion is composed of an assembly of an upper skin and a lower skin hermetically connected to each other along junction lines in order to delimit only the central portions of the cells.
2. The casing of claim 1, wherein the cells comprise a several pairs of cells, each pair of cells comprising: a departure cell defining a circulation of the liquid culture medium from an upstream portion of the casing to a downstream portion of the casing, where one of the departure cells is in fluid connection with the first opening disposed in the upstream portion of the casing; and a return cell defining a circulation of the liquid culture medium of said downstream portion towards said upstream portion, where one of the return cells is in fluid connection with the second opening disposed in the upstream portion of the casing; and wherein the junction elbows alternately include downstream junction elbows disposed in the downstream portion of the casing and upstream junction elbows disposed in upstream portion of the casing to join pairwise the departure and the return cells.
3. The casing according to claim 1, wherein each cell is delimited by two rectilinear junction lines to define a rectilinear portion of the flow path, wherein said rectilinear junction lines present free ends, and each inflatable junction elbow is delimited at least outwardly by a bent junction line substantially bent at 180 and exhibiting two ends connected to two rectilinear junction lines delimiting two adjacent cells.
4. The casing according to claim 3, wherein the ends of each bent junction line or the free ends of the rectilinear junction lines located inside corresponding inflatable junction elbows are provided with reinforcing elements hermetically crossing the two membranes.
5. The casing according to claim 3, wherein each bent junction line exhibits either an overall shape of a semicircle, or an overall shape of a broken line formed of several rectilinear segments.
6. The casing according to claim 1, wherein the casing is made up of only the upper and lower membranes, said membranes being hermetically connected to each other along junction lines alternately delimiting the inflatable cells and the inflatable junction elbows.
7. The casing according to claim 1, wherein the cells define rectilinear portions of the flow pathway which are parallel to a longitudinal direction of the casing and are of substantially equivalent lengths.
8. The casing according to claim 1, wherein the junction lines comprise at least one of welding, sewing, gluing or clipping lines between the two membranes.
9. A photosynthetic reactor suitable for the culture of photosynthetic microorganisms, comprising: at least one reaction casing according to claim 1; at least one closing pipe ensuring fluid connection between the first and second openings of said reaction casing; at least one circulation device disposed in said closing pipe and designed to put into circulation the liquid culture medium in the closing pipe and the reaction casing; at least one liquid injector disposed in said closing pipe and designed to allow injection of the liquid in the reaction casing; at least one gas injector disposed in said closing pipe and designed to allow injection of the gas in the reaction casing; at least one liquid outlet for harvesting the culture of photosynthetic microorganisms; and at least one gas exhaust disposed in said closing pipe and designed to allow the release of gas injected in the reaction casing.
10. The photosynthetic reactor according to claim 9, wherein the closing pipe exhibits a globally U or V shape and comprises: an ascending portion provided with a high portion in fluid connection with the first opening; and a descending portion provided with a high portion in fluid connection with the second opening; the descending portion and the ascending portion being provided with respective low portions in fluid connection, and the circulation device being designed for putting the liquid culture medium in circulation in the reaction casing from the first opening to the second opening.
11. The photosynthetic reactor according to claim 10, wherein the liquid injector is disposed in the high portion of the ascending portion, and the gas injector is disposed in the ascending portion, in the high portion or the low portion of said ascending portion.
12. The photosynthetic reactor according to claim 11, wherein the circulation device is constituted of a gas lift device and comprises a gas injection duct disposed in the low portion of the ascending portion, said gas injection duct also constituting the gas injecting injector.
13. The photosynthetic reactor according to claim 11, wherein the gas exhaust and the liquid outlet are constituted of a common duct disposed in the high portion of the descending portion, said common duct being provided with a free end set at an adjustable height with respect to the expanse of water.
14. The photosynthetic reactor according to claim 11, wherein the gas exhaust is constituted of a first outlet duct disposed in the high portion of the descending portion, and the liquid outlet is constituted of a second outlet duct disposed in the low portion of either one of the ascending or descending portions and provided with a free end set at an adjustable height with respect to the expanse of water.
15. The photosynthetic reactor according to claim 10, wherein the liquid injector is disposed in the high portion of the ascending portion, and the gas injector is disposed in the descending portion, and wherein the gas exhaust is constituted of a first outlet duct disposed in the high portion of the ascending portion and the liquid outlet is constituted of a second outlet duct disposed either in the high portion of the descending portion or in the low portion of either one of the ascending or descending portions, said second outlet duct being provided with a free end set at an adjustable height with respect to the expanse of water.
16. The photosynthetic reactor according to claim 10, wherein the circulation device is constituted of a mechanical circulation device, said mechanical circulation device being disposed in the ascending portion.
17. A method for manufacturing a reaction casing according to claim 1, comprising the following steps: providing an upper membrane and a lower membrane achieved at least partially of a material that is flexible, watertight and transparent to light radiation; hermetically connecting said membranes along junction lines delimiting the inflatable cells; and providing inflatable junction elbows made at least partially from a flexible and watertight material and joining pairwise said cells to define a flow pathway of generally sinuous shape between a first opening and a second opening provided in said casing, wherein the casing is composed of three distinct portions comprising an upstream portion, a downstream portion and a central portion interposed between the upstream and downstream portions, wherein: the upstream portion is composed of an assembly of an upper skin and a lower skin hermetically connected to each other along junction lines in order to delimit both upstream portions of the cells and upstream inflatable junction elbows, the two openings being provided in this upstream portion; the downstream portion is composed of an assembly of an upper skin and a lower skin hermetically connected to each other along junction lines in order to delimit both downstream portions of the cells and downstream inflatable junction elbows; and the central portion is composed of an assembly of an upper skin and a lower skin hermetically connected to each other along junction lines in order to delimit only the central portions of the cells.
18. A photosynthetic microorganism cultivation method, using a reactor according to claim 9, and comprising the following steps: injecting a liquid culture medium in the reaction casing according to a flow rate controlled with the liquid injector; injecting a gas into the reaction casing according to a flow rate controlled with the gas injector; circulating the liquid culture medium with the circulation device; controlling the circulation device and the gas injector in order to establish in the reaction casing a two-phase gas/liquid culture medium flow regime.
19. The method according to claim 18, wherein the controlling step comprises a step of controlling the velocity of the circulation of the liquid in the reaction casing between 0.1 and 1.0 m/s, and a step of controlling the velocity of circulation of the gas in the reaction casing between 0.5 and 2.0 m/s.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
(1) Other characteristics and advantages of the present invention will appear after reading the detailed description hereinafter, the several non-limiting examples of embodiments, made with reference to the drawings in which:
(2)
(3)
(4)
(5)
(6)
(7)
(8)
(9)
(10)
(11)
(12)
(13)
(14)
(15)
(16)
(17)
DETAILED DESCRIPTION
(18) The description of a reaction casing 1 according to the invention, according to a first assembly mode, for a photosynthetic reactor 2 or photobioreactor, is made with reference to the
(19) The casing 1 is designed, on one hand, to float on a water body and, on the other hand, to delimit a path for a gas/liquid culture medium to flow in two phases between a first opening 11 and a second opening 12 of the casing 1.
(20) This inflatable casing 1, with an elongated shape along a main longitudinal axis, is made by assembly of an upper membrane 31 and a lower membrane 32 made from a flexible material, hermetically sealed and transparent to light radiation. The casing 1 has two opposite longitudinal edges 13, 14 extending along the main longitudinal axis, and two opposite lateral edges, respectively, upstream lateral edge 15 and downstream lateral edge 16, extending along a secondary lateral axis; the upstream lateral edge 15 being hence disposed in a portion called upstream of the casing 1 while the downstream lateral edge 16 is disposed in an opposite portion called downstream. The two openings 11, 12 are arranged on the upstream lateral edge 15, and thus in said upstream portion, and spaced apart from one another, with the first opening 11 arranged in the vicinity of a longitudinal edge 13 while the second opening 12 is arranged in the vicinity of the other longitudinal edge 14.
(21) These membranes 31, 32 are substantially equivalent in size and shape, and have in particular an overall rectangular shape with a longitudinal dimension (or length) greater than the lateral dimension (or width). The membranes 31, 32 have in particular a length of several tens of meters long, for example in the order of 50 meters.
(22) The membranes 31, 32 are hermetically connected to each other according to junction lines 41, 42 delimiting alternately: inflatable cells 33, 34; and inflatable junction elbows 35, 36 joining pairwise the cells 33, 34 in order to define a unique flow path with an overall sinuous shape.
(23) In this way, the cells 33, 34 which extend along the main longitudinal axis, in other words in the direction along the membranes 31, 32, are connected end-to-end or communicate together by junction elbows 35, 36 to form together a single coil-shaped pipe.
(24) The cells 33, 34 comprise a plurality of pairs of cells, for example, five pairs of cells in the example of
(25) Thus, the number of cells 33, 34 is even such that the first opening 11 (inlet of the liquid medium in the casing 1) and the second opening 12 (outlet of the liquid medium in the casing 1) are on the same upstream lateral edge 15. The upstream lateral edge 15 is thus provided with the two openings 11, 12, with eventually a lateral junction line 47 extending between the two openings 11, 12, while the downstream lateral edge 16 is hermetically closed by at least one lateral junction line 47 in addition to the junction lines 42.
(26) The junction elbows 35, 36 alternately comprise: downstream junction elbows 36 disposed in downstream portion of the casing 1 and joining by 180 a departure cell 33 and a return cell 34 of a same pair of cells 33, 34; and upstream junction elbows 35 disposed in upstream portion of the casing 1 and joining by 180 a return cell 34 and a departure cell 33 of two adjacent pairs of cells 33, 34.
(27) Thus, the sinuous flow path is delimited successively, from the first opening 11 to the second opening 12, through the first departure cell 33, the first downstream junction elbow 36, the first return cell 34, the first upstream junction elbow 35, the second departure cell 33, and so on until the last downstream junction elbow 36 and finally the last return cell 34.
(28) Each cell 33, 34 is delimited by two rectilinear junction lines 41 to define a rectilinear portion of the rectilinear flow path, and thus the cells 33, 34 define the rectilinear portions of the flow path which are parallel to the main longitudinal axis of the casing 1 and which have substantially equivalent lengths.
(29) The length of a casing 1 depends on the lake and on fluid supplies. With the number of cells 33, 34, this length will determine the productivity of the reactor. For example, based on an average productivity of 10 g/m.sup.2/day, a casing of 50 m long with four cells operating eight months a year should be able to produce 80 kg of dry matter per year.
(30) As shown in
(31) Once inflated, and as shown in
(32)
(33) Therefore, between deflation and inflation, the rectilinear junction lines 41 approach a distance equivalent to D (/21). Furthermore, the bent junction lines 42 make a 180 turn.
(34) A difficulty in performing these turns, in other words these bent junction lines 42, is that these lines undergo, on one hand, a deformation between performing the lines 42 and the flat cuttings and, on the other hand, a tubular deformation they adopt during inflation, of folds resulting from narrowing of the junction lines 41, 42 during inflation.
(35) An embodiment of the bent junction lines 42 is hence provided which limits the folds in order to reduce the concentrations of tension that could create zones of fragility on the membranes 31, 32, as well as to avoid zones of retention of the culture difficult to access for cleaning bodies where biomass could accumulate.
(36) In a first embodiment shown in
(37) In a second embodiment shown in
A=Arctan(12/)=19.97.
(38) As for the central rectilinear segment, it is perpendicular to the main longitudinal axis and located at a distance at least equal to D/2 from the free end 45, 46 of the concerned rectilinear junction line 41 at the deflated state. Two intermediate rectilinear segments join this rectilinear central segment to the respective end rectilinear segments to form cut-off corners or segments of adjacent lengths.
(39) Similarly, in the embodiment of
(40) This minimum width of D/2 for the junction elbows 35, 36 in their plane of symmetry is advantageous so that the distance between the floor and the ceiling, once the casing 1 is inflated, is sufficient to pass the cleaning bodies.
(41) This second embodiment does not completely avoid the formation of folds but it reduces it; such folds resulting from the inevitable approaching of the rectilinear junction lines 41, but the elasticity of the material used for the membranes 31, 32 allows to limit them.
(42) In a third embodiment shown in
(43) Other embodiments may be considered to limit the formation of folds, but this needs radial junction lines and cuts in the turns that substantially complicate the assembly of the membranes 31, 32.
(44) It is to be noted that concentrations of tension can occur due to inflation, particularly at the ends of the junction lines 41, 42 separating two communicating cells 33, 34, that is to say, at the ends 43 of bent junction lines 42 and at the free ends 45, 46 of rectilinear junction lines 41 located inside the corresponding junction elbows 35, 36. These ends 43, 45, 46 are therefore advantageously reinforced by reinforcement means of the eyelet, rivet or bolt type passing through the membranes 31, 32, in the manner of padding punctures. These reinforcement means should strongly apply membranes 31, 32 against one another so that their assembly remains hermetically sealed despite their piercing.
(45) Concerning the assembly of the two membranes 31, 32, the junction lines 41, 42 are of the welding, sewing, gluing or clipping type between the two membranes 31, 32. Thus, the back-and-forth path defined by the casing 1 is arranged by the junction lines 41, 42 by means of welds, seams, gluing or of clipping devices. Of course, other hermetic assembly techniques of flexible membranes can be considered, in particular the combination of the aforementioned techniques.
(46) In the case of an assembly by heat sealing, it is possible to make the membranes 31, 32 pass in front of the heating devices equipping rotary machines. Generally, this assembly should preferably be done in a microbiologically controlled atmosphere so that casings can be presumed sterile at the end of manufacturing.
(47) Regarding the packaging of a casing 1 in assembly outlet, it is possible to wind the casing 1 around an axis or floating mandrel F used for handling and ensuring the buoyancy of the casing 1 during its unwinding by inflation on the lake, as described hereinafter. The winding of the casing 1 is such that the downstream lateral edge 16 is located inside the winding, while the upstream lateral edge 15 is located in the periphery of the winding to ensure hermetic connection of the two openings 11, 12 with a closing pipe described subsequently. The winding of the casing 1 which therefore follows the assembly thus starts from only the downstream lateral edge 16 so that the upstream lateral edge 15 remains accessible for connection to the closing pipe. Once the casing 1 is unwound on the expanse of water, the downstream lateral edge 16 is free or eventually maintained while the upstream lateral edge 15 is hermetically connected with this closing pipe in order to complete the circuit.
(48) The two membranes 31, 32 are made of a flexible material, in other words, a material suitable for folding, inflation, transverse deformation and/or deflection of the cells 33, 34.
(49) Concerning the membranes 31, 32, the material should make it possible for them to resist: the tension they are subjected to during inflation of the casing 1, and for this purpose, an overpressure which will be applied inside the casing 1 should not exceed a value to be set; the tensile force related to displacement of the mass of the expanse of water, that of the air being negligible; and the return force of the mooring of the casing 1 onto a mooring support (floating craft E described hereinafter, at wharf or dock).
(50) The Applicant has thus established a list of plastic materials used for the manufacture of membranes 31, 32, including in particular polyethylene, polypropylene, polyamides (nylon, Rilsan), polytetrafluoroethylenes (PTFE), either in the form of a membrane, in the form of woven fibers or in the form of composite fabrics, calendered or coated.
(51) This list is obviously not limitative and may in particular, be supplemented with new transparent materials appearing on the market.
(52) The multilayer co-extrusion can be used for example to make these membranes 31, 32 which are commonly used in agriculture. It is also advantageous to provide slightly extensible materials in order to allow for end deformations and limit the formation of creases.
(53)
(54) Each reactor 2 includes: at least one casing 1 in accordance with to the invention; at least one closing pipe 5 ensuring the fluid connection between the first 11 and the second opening 12 of the casing 1, said openings 11, 12 being hermetically connected to said closing pipe 5; at least one circulation means 6 disposed in the closing pipe 5 and designed for allowing the liquid culture medium L to circulate within the closing pipe 5 and within the casing 1; at least one liquid injection means 71 disposed in the closing pipe 5 and designed to allow the injection of the liquid L in the casing 1; at least one gas injection means 72 disposed in the closing pipe 5 and designed to allow the injection of the gas G in the casing 1; at least one liquid outlet means 81 for harvesting the culture of photosynthetic microorganisms, such as a fluid; at least one gas exhaust means 82 disposed in the closing pipe 5 and designed to allow the escape of the gas G injected into the casing 1.
(55) The reactor 2 may comprise two distinct liquid injection means allowing injecting respectively the liquid culture medium and the inoculum in the reactor 2. These injection means may come in the form of injection ports allowing a connection to a source with asepsis control.
(56) The reactor 2 may also comprise: one or more sensors (not illustrated) disposed on the closing pipe 5 and suitable for providing the signals required for the control of the reaction, in particular, signals representing physical, chemical, or biological parameters of the quality of the culture, such as temperature, pH, rate of dissolved oxygen and turbidity of the liquid medium, etc. these controls are used in particular, to regulate injections of gas and liquid into the reactor 2; means for controlling sterility (not illustrated) of gaseous and liquid media entering into and out from the space confined by the reactor 2, in particular some filters intended to prevent the access of contaminants; regulation loops (not illustrated) interposed on the circuit of the culture and intended to regulate the main nutrient intakes of the culture, in particular, the admission of sterile medium by the concentration of dry matter, pH by the injection of CO.sub.2.
(57) The closing pipe 5 ensures closing the looping fluid path between the first 11 and the second openings 12 of the casing 1. The closing pipe 5 is made of a material that can be non-transparent to solar radiation and/or may be disposed away from light inside a closed area or a closed craft E as shown in particular, in
(58) The closing pipe 5 exhibits a generally U or V shape and comprises: an ascending portion 51 provided with a high portion or end 510 in fluid connection 30 with the first opening 11; and a descending portion 52 provided with a high portion or end 520 in fluid connection with the second opening 12.
(59) The ascending portion 51 and the descending portion 52 are provided with respective low portions or ends 511, 512 in fluid connection. This general U or V shape of the closing pipe 5 is intended to block the gas upstream of the closing pipe 5, that is to say at the output of the casing 1 at the second opening 12, in order, on the hand, to avoid its recycling and, on the other hand, to prevent it from reducing the performance of the flowing of the liquid.
(60) The ascending portion 51 and descending portion 52 are preferably tubular with an inner diameter substantially equivalent to the diameter D. The liquid injection means 71, such as a liquid pipe or liquid port, is disposed in the high portion 510 of the ascending portion 51, at the input of the casing 1 at the first opening 11.
(61) In the embodiments of
(62) In the embodiments of
(63) In this embodiment, the common pipe 81, 82 is placed in the high portion 520 of the descending portion 52, just before the descending portion 52 which plunges downwards, in order to evacuate the gas blocked in the descending portion 52.
(64) In a non-illustrated embodiment, the gas exhaust means 82 comprises a first output pipe disposed in the high portion 520 of the descending portion 52, and the liquid outlet means 81 comprises a second output pipe disposed in the low portion 511, 521 of either one of the descending 51 and ascending 52 portions so as to only take the liquid L. The location in low portion of this second outlet pipe 81, where the culture is harvested, is chosen so as to minimize the influence of the injection of liquid media that would result in its dilution.
(65) This second outlet pipe 81 is advantageously provided with a free end set at an adjustable height with respect to the expanse of water in order to establish the inflation overpressure. In this embodiment, the outlet orifice towards the first outlet pipe 82 is preferably provided with a closure with float intended to prevent the passage of liquid.
(66) Thus, the output of the volume of surplus liquid L in the reactor 2 is carried out by this overflow comprising the common pipe 81, 82 in communication with the closing pipe 5. The point of communication of this common pipe 81, 82 with the closing pipe 5 is placed at the output of the casing 1, upstream of the sterile liquid injection means 71, as illustrated in
(67) This common pipe 81, 82, forming an overflow, constitutes a breakage of the containment of the liquid culture medium L. In order to avoid the retro-contamination of the culture in progress, the common pipe 81, 82 can usefully have a length of several meters, before opening into a harvesting tank, and be kept sterile by periodic cleaning.
(68) As introduced hereinabove, the emergence of creases must be avoided, with an appreciable reduction of the reaction volume. In fact, such creases could cause surges and other hammerings in the case of agitation of the expanse of water, which would lead to brutal tension of the flexible material of the casing 1 liable to make it tear.
(69) For this reason, the casing 1 must be continuously stretched using an inflation overpressure; a checking this overpressure should allow to maintain the casing 1 at a nominal level compatible with the agitation of the expanse of water and proper functioning of the assembly.
(70) Thus, a method for cultivating photosynthetic microorganisms using such a casing 1 comprises a step of pressurizing the casing 1 consisting in creating an inflation overpressure within this casing 1.
(71) The inflation overpressure of the casing 1 determines, as described hereinabove, its stiffness, in other words its resistance to deformation related to the agitation of the expanse of water, and the influence of the latter on the two-phase inner flow of the gas G and the liquid L; this overpressure being equal to the sum of the pressures of the gas and the liquid in the volume of the casing 1.
(72) The control of the inflation overpressure also relates to detecting leaks. The output of surplus gas volume is carried out by gas exhaust means 82 described hereinabove and provided with an orifice set in high portion 520 of the descending portion 52. The gas is, for example, channeled to a filter that prevents the retro-contamination of the reactor before being released into the atmosphere or recycled. A control of the gas output rate may be carried out, by means of a means for controlling the gas flow rate, such as a needle valve, in order to adjust the height of the meniscus (liquid level or gas/liquid interface) in the casing 1.
(73) In the embodiments described hereinabove and illustrated in
(74) In non-illustrated embodiments, it is possible to provide a counter-current circulation which is implemented with the liquid entering the casing 1 through the first opening 11 and exiting the casing 1 through the second opening 12 and with the gas entering the casing 1 through the second opening 12 and exiting the casing 1 through the first opening 11, in such a manner that the liquid and gas flow in the casing in opposite flow directions. To this end, the liquid injection means 71 remains positioned at the input of the casing 1, in the high portion 510 of the ascending portion 51, while the gas injection means 72 is disposed at the output of the casing 1, in the descending portion 52 (high or low portion) so that the gas enters the casing 1 through the second opening 12. Furthermore, the liquid outlet means 81 remains positioned at the exit of the casing 1 (either in the high portion 520 of the descendant portion 52, or in low portion of the ascending portion 51 or the descending portion 52) still with a free end set at an adjustable height with respect to the expanse of water, whereas the gas exhaust means 82 is positioned at the input of the casing 1, in the high portion 510 of the ascending portion 51.
(75) In general, the gas exhaust means 82 is designed for venting the gas or for recycling the gas through a filter to avoid the retro-contamination of the reactor 2. Preferably, the gas exhaust means 82 is fashioned in such a manner as to maintain the head loss through this filter as low as possible in order to control the pressure in the casing 1.
(76) The circulation means 6 is designed for allowing the liquid culture medium L to circulate in the reaction casing of the first opening 11 towards the second opening 12, this liquid culture medium L thus flowing in the closing pipe 5, from the high portion 520 of the descending portion 52 to the high portion 510 of the ascending portion 51. Preferably, the circulation means 6 is chosen in such a manner as to generate reduced shearing and centrifugal forces. However, it is possible to use all types of pumping means and in particular, centrifugal pumps without departing from the scope of the invention.
(77) In the first embodiment of the reactor 2 illustrated in
(78) In the two other embodiments illustrated in
(79) In the second embodiment of the reactor 2 illustrated in
(80) Advantageously, the housing 512 of the propeller 62 is disposed between a divergence area and a convergence area of the closing pipe 5, in order to ensure hydraulic continuity without sudden variation of velocity, thus, in order to limit head losses, accelerations and the shearing forces undergone by the microorganisms.
(81) According to an advantageous feature and as illustrated in
(82) Furthermore, and as shown in
(83) In the second embodiment of the reactor 2 illustrated in
(84) The reactor 2 may in fact include one or several cleaning elements (not illustrated) fashioned in such a manner as to circulate along the flow path, in other words inside the casing 1 and the closing pipe 5, in order to clean the inside of the casing 1 and closing pipe 5. In order to flow in a loop in the reactor 2, the cleaning element or elements are also fashioned in such a manner as to pass through the means 6 allowing the liquid culture medium to circulate, for example through the blades of the propeller 62 or through the turbine 65.
(85) The cleaning element or elements, preferably spherical, exhibit for example a diameter lower than or substantially equal to the internal diameter D of the closing pipe 5 and the cells 33, 34 in order to optimize the cleaning of the internal walls of the closing pipe 7.
(86) The difference in velocity between the gas flow and the liquid flow directly affects the gas/liquid mass transfers and must advantageously be maintained at the highest possible level. This is the reason why every cleaning element must not prevent the passage of gas. For this reason, each cleaning element is fashioned to let the gas pass at least partially inside the reactor 2 while being suitable for being driven by the circulation of the liquid culture medium so that the cleaning element does not affect the velocity difference between the gas and the liquid medium. To this end, the cleaning element or each cleaning element is made in the shape of a brush, in particular, spherical, comprising an assembly of hairs, bristles, strands or equivalent, with a central portion bearing these hairs. Thus, in the horizontal casing 1, the emerged hairs let the gas pass at the gas meniscus and the submerged central portion and the hair bearer exhibits a sufficiently large diameter for forming an obstacle to the passage of liquid such that the liquid medium draws the cleaning element with it.
(87) In the same manner, the cleaning element can be achieved in the shape of a hollow sphere from elastomeric material. Advantageously, a substantial portion of the surface of the sphere can be pierced with holes which allow the gas to pass through. Certain cleaning elements may also present a density greater than that of the water in order to stay in contact with the floor of the casing 1 corresponding to its fully submerged lower or low portion.
(88) The flexibility or elasticity of the cleaning elements further allow to reduce the culture retention areas which would result from the emergence of creases in the cells 33, 34 and/or the junction elbows 35, 36.
(89) As shown in
(90) Regarding the two-phase flows in horizontal pipes, certain works have indeed highlighted states of flow according to conditions of velocity, diameter, temperature, nature, pressure of circulating fluids, namely, in particular: dispersed bubbles flow, of Mandhane AD typology; and elongated bubbles flow, of Mandhane I typology; stratified flow and wavy stratified flow and smooth stratified flow, of Mandhane SS and SW typology; slug flow, of Mandhane I typology; annularmist flow, of Mandhane AD typology.
(91) In the case of the present invention, the privileged states of flow are hence, located at the SS/I transition in the Mandhane typology, that is to say, between the stratified state and the slug state or elongated bubble state.
(92) In the stratified regime, the gas/liquid interface comprises the free surface, the width of which varies with the level of liquid in the casing 1. In the slug or elongated bubbles state, the gas/liquid interface comprises the floor and ceiling of the slug or the elongated bubble. The mass transfers being proportional to the length of the path, the effect thereof on the performance of the reaction are reduced, which allowing considering large scale increases. The casings 1 can thus exhibit lengths of several hundred meters.
(93) As shown in
(94) This craft E may comprise a closed space, or technical space, in which are disposed, sheltered from bad weather, the closing pipe 5 and circulation means 6, and a wall or freeboard FB on which the closing pipe 5 is fixed.
(95) As shown in
(96) In a first embodiment shown in
(97) In a second embodiment shown in
(98) This mooring AG comprises links LG connecting the downstream lateral edges 16 of the casing 1 to a rudder PL perpendicular to the cells 33, 34, and a mooring point PG achieved in particular, in the shape of a beam or rod planted vertically in the bottom of the expanse of water, to which the rudder PL is connected. The adjustment of the length of the links LG and their parallel disposition allows to uniformly distribute the mooring tensions uniformly between all casings 1.
(99) In case the water is navigable, diurnal and nocturnal signaling means in accordance with local legislations may equip the different points of this floating assembly, and in particular, the downstream lateral edges 16 of the casings 1.
(100) Furthermore, and as illustrated in
(101) The placement of the casing 1 is described hereinafter with reference to
(102) As shown in
(103) As shown in
(104) Once the casing 1 is unwound and inflated, the circulation of the liquid medium L can be established in the reactor 2, by starting up the circulation means 6. The gas circulation in the casing 1 creates positive buoyancy distributed homogeneously along the length of the casing 1 which maintains them at the surface. The horizontality is ensured naturally by the sustenance of the expanse of water and by the gas circulation. A hatch TR may be provided on the craft E to isolate the outer portion of the reactor 2 from the inner portion of the reactor 2 once the casing 1 is unwound and inflated.
(105) In the embodiments illustrated in the figures, the closing pipe 5 and circulation means 6 are disposed outside the craft E, this is known as a solution called outboard. It is of course possible that the closing pipe 5 exhibits an inner portion, disposed inside the craft E with the circulation means 6 advantageously disposed in this inner portion, and an outer portion disposed outside the craft E and connected to the casing 1; This is known as a solution called inboard. In this inboard solution, it is possible to allow the outer portion of the closing pipe 5 to be movable with respect to the inner portion in a raised position in order to be able to get it out of the water.
(106) As illustrated in
(107) In the embodiment illustrated in
(108) The first connection pipe 91 links: an inlet point disposed on the first reactor 2 downstream of the circulation means 6, such that the rotating propeller 62, in the high portion 510 of the ascending portion 51; to an outlet point disposed on the second reactor 2 upstream of the circulation means 6 of this second reactor 2, in the low portion 511 of the ascending portion 51.
(109) The second connecting pipe 92 links: an inlet point disposed on the second reactor 2 downstream of the circulation means 6, in the high portion 510 of the ascending portion 51; to an outlet point disposed on the first reactor 2 upstream of the circulation means 6 of this second reactor 2, in the low portion 511 of the ascending portion 51.
(110) The reactors 2 are assembled in a parallel manner to form a coherent and productive assembly. In order to make the inoculation of a reactor by its neighbor possible, the microorganism concentration of which would have reached an advanced stage, the assembly allows the interconnection of these two reactors with the connection pipes 91, 92 such that their respective contents are mixed. Furthermore, the outlet points of the connection pipes 91, 92 are placed at the end of the convergence areas upstream of the corresponding housing 512 to benefit from a Venturi effect.
(111) The valves 93, 94, 95, 96 allow the aseptic connection of the two connection pipes 91, 92 which connect crosswise and symmetrically the inlet points and outlet points of the two reactors 2 to be interconnected. The valves 93, 94, 95, 96 are substantially disposed at inlet and outlet points of the corresponding connection pipes 91, 92.
(112) The use of such an assembly can be done in the following manner in order to proceed with the inoculation of the second reactor 2 (right) from the first reactor 2 (left) already in service when the concentration of microorganisms has reached the level of exploitation.
(113) First, the valves 93, 94 and their counterparts 95, 96 are closed, the first reactor 2 is in operation with the establishment of the circulation inside this first reactor, and the second reactor 2 to be inoculated is filled with a sterile nutritive medium. Second, the circulation is established inside the second reactor 2 and the valves 93, 94 and their counterparts 95, 96 are opened to establish a cross exchange between the two reactors as illustrated by the arrows EC in
(114) As illustrated schematically in
(115) In a parallel configuration, the closing pipe comprises a collecting duct in which circulation means is disposed, and a plurality of distribution ducts connected, on one hand, to the collecting duct and, on the other hand, to the respective casings 1, such that the liquid medium is collected at the outlet of casings 1, passes through the circulation means and then is distributed at the inlet of the casings 1.
(116) In a series configuration, the second opening 12 (outlet of the liquid medium) of a casing 1 is directly connected to the first opening 11 (inlet of the liquid medium) of the following casing 1, and the closing pipe is disposed between the first opening 11 of the first casing and the second opening 12 of the last casing.
(117) In the parallel configuration, the cleaning efficiency is however, not optimal as the cleaning elements are distributed randomly in the casings 1. That is why a series configuration is preferred where the cleaning elements must follow a single path, from casing to casing.
(118) The method for cultivating photosynthetic microorganisms, in particular algae, using a reactor 2 in accordance with the invention comprises the following steps: injecting a liquid culture medium L in the reaction casing 1 according to a flow rate controlled by the liquid injection means 71; injecting gas G in the reaction casing 1 according to a flow rate controlled by the gas injection means 72; pressurizing the casing 1 comprising creating an inflation overpressure in this casing 1 to ensure the buoyancy and the deployment thereof; circulating the liquid culture medium with the circulation means 6; controlling the circulating means 6 and the gas injection means 72 in order to establish in the reaction casing 1 a two-phase gas/liquid culture medium flow regime of the stratified flow or of the slug or elongated bubbles flow type; and recovering photosynthetic microorganisms with the liquid outlet means 81.
(119) During the travel in the casing 1, the liquid medium containing the photosynthetic microorganisms receives solar radiation through the transparent material of the membranes 31, 32 of the casing 1, exchanges heat with the body of water by diffusion, mixture and conduction through this same material, and exchanges components with the gas G through their common interface. The production capacity mainly depends on the length of the casing 1 and the number of cells 33, 34.
(120) Advantageously, the circulation velocity of the liquid in the casing 1 ranges between 0.1 and 1.0 m/s.
(121) Furthermore, the circulation velocity of the gas is established between about 0.5 and 2.0 m/s, corresponding to an adequate velocity regime for the flow rates required for the reaction.
(122) Still more advantageously, the circulation means 6 comprising a propeller 62 driven in rotation by a motor 63 is controlled in such a manner that the speed of rotation of the propeller 62 is less than about 1000 rpm, in order to limit the mechanical stresses within the liquid culture medium.
(123) The invention also relates to a method for manufacturing a casing 1 according to the first assembly mode or the second assembly mode (described hereinafter) and comprising the following steps: providing an upper membrane 31 and a lower membrane 32 made at least partially from a material which is flexible, watertight and transparent to light radiation; covering the lower membrane 32 with the upper membrane 31; hermetically connecting the two membranes 31, 43 according to junction lines 41, 42 alternately delimiting inflatable cells 33, 34 and inflatable junction elbows 35, 36 pairwise joining the cells 33, 34 to define a flow path with a globally sinuous form between the two openings 11, 12, the two openings 11, 12 being provided at the ends of this path; and winding the casing 1 around a drum to form a bobbin B.
(124) In the first assembly mode described hereinabove with reference to
(125) In a first embodiment of the first assembly mode of the casing 1, the two membranes 31, 32 are composed of two sheets (otherwise known as films), distinct and separated from one another before assembly, the assembly consisting afterwards in interconnecting them thanks to the junction lines 41, 42, 47. In this embodiment, the lower membrane 32 is not necessarily transparent to solar radiation, solely the upper membrane 31 should imperatively be transparent.
(126) In a second embodiment of the first assembly mode, the two membranes 31, 32 are composed of one and the same sheet 5 (otherwise known as film) which is folded in two in the direction of its length along a longitudinal folding line, then the junction lines 41, 42, 47 are made to hermetically connect both sides of the sheet and thereby form the casing 1; the longitudinal folding line can possibly constitute a junction line 41.
(127) In a second assembly mode described with reference to
(128) The upstream portion 100 is composed of an assembly of an upper skin (otherwise known as sheet or film) and a lower skin hermetically connected to each other along junction lines 41, 42, 47 in order to delimit both the upstream portions 330, 340 of the cells 33, 34 and the upstream junction elbows 35, the two openings 11, 12 being provided in this upstream portion 100.
(129) The downstream portion 101 is composed of an assembly of an upper skin and a lower skin hermetically connected to each other along junction lines 41, 42, 47 in order to delimit both the downstream portions 331, 341 of the cells 33, 34 and the downstream junction elbows 36.
(130) The central portion 102 is composed of an assembly of an upper skin and a lower skin hermetically connected to each other along junction lines 41 in order to delimit only central portions 332, 342 of the cells 33, 34. The central portion 102 exhibits a length equivalent to at least 90% of the total length of the casing 1.
(131) Thus, the three portions 100, 101, 102 are made independently from each other, then joined end to end to form the casing 1; the upper membrane 31 of the casing 1 comprising the assembly of the upper skins of these portions 100, 101, 102, while the lower membrane 32 of the casing 1 comprises the assembly of the lower skins of these portions 100, 101, 102. The end to end junction of these three portions 100, 101, 102 is performed so that the junction lines 41 of the various portions are aligned and continuous.
(132) The main advantage of this embodiment with three portions is to facilitate the realization of the elbows 35, 36 to guarantee their geometries. In fact, it is important to respect the geometry described hereinabove for the elbows 35, 36, and in particular, the minimum width of the elbows 35, 36 in their respective planes of symmetry, which requires the control of the interruption of the junction lines 41 at their free ends 45, 46. When working with short end portions 100, 101, it is thereby easier to control the localization and the interruption of the junction lines 41 and guarantee the required geometry.
(133) Furthermore, it is possible to choose skins that are not transparent to solar radiation in order to make the end portions 100 and 101, thus, allowing to possibly select more suitable materials for achieving bent junction lines 42 and interruptions in the rectilinear junction lines 41 Only the central portion 102 must be made of a material transparent to solar radiation, or even only the upper skin of the central portion 102 must be made from a material transparent to solar radiation. The central portion 102 may be made by assembling two separate sheets, or by folding one same and single sheet.
(134) In the embodiments described above with reference to
(135) In a variant alternative shown in
(136) In a third assembly method described with reference to
(137) In the assembly method illustrated in
(138) To report the rigid elbows 35, 36, the latter preferably exhibit nozzles 350, 360 which are introduced inside the ends of the cells, and then pipe clamps 351, 361 are tightened around these nozzles 350, 360 to guarantee permeability.
(139) Likewise, stiff inlet 37 and outlet 38 ducts maybe attached to the openings 11, 12 of the casing 1; these rigid inlet 37 and outlet 38 ducts preferably exhibiting nozzles 370, 380 which are introduced inside the openings 11, 12, then pipe clamps 371, 381 are tightened around these nozzles 370, 380 to guarantee a permeability.
(140) Furthermore, it may be considered stiff downstream junction elbows 36 be gathered within one same stiff downstream piece 391 and that the stiff upstream junction elbows 35 and the stiff ducts 37, 38 be gathered within a same rigid upstream piece 390.
(141) Of course, the aforementioned implementation example does not exhibit any limiting nature and other improvements and details may be attached to the casing, reactor and methods according to the invention, without nevertheless departing from the scope of the invention where other forms of membrane and/or cell and/or the junction elbow and/or the closing pipe can for example be carried out.