CELLULOSE-ADIPATE-#-CYCLODEXTRIN STRUCTURE, AND PREPARATION METHOD THEREFOR

Abstract

The present invention relates to a structure composed of -cyclodextrin, a linker and a supporter, and a preparation method thereof. The cellulose-adipate--cyclodextrin structure of the present invention is effective in removing cholesterol from foods, and the use of the cellulose-adipate--cyclodextrin structure may reduce the onset of adult and chronic diseases due to excessive intake of cholesterol, thereby contributing to maintenance, recovery and promotion of health.

Claims

1. A cellulose-adipate--cyclodextrin structure, wherein one carboxyl group of adipic acid is linked to microcrystalline cellulose via an ester bond and another carboxyl group remaining in the adipic acid is linked to -cyclodextrin via an ester bond.

2. The cellulose-adipate--cyclodextrin structure according to claim 1, wherein the microcrystalline cellulose is puffed.

3. The cellulose-adipate--cyclodextrin structure according to claim 1, wherein the microcrystalline cellulose is 0.1 to 1.5 mm in size.

4. A method of preparing a cellulose-adipate--cyclodextrin structure, the method comprising (A) a step of preparing an intermediate by reacting microcrystalline cellulose with adipic acid under alkaline conditions; (B) a step of preparing a cellulose-adipate--cyclodextrin complex by adding -cyclodextrin to the intermediate after step (A); and (C) a step of washing and collecting the complex.

5. The method according to claim 4, wherein, in step (A), the microcrystalline cellulose and the adipic acid are added in a weight ratio of 1:1 to 1:10 and reacted.

6. The method according to claim 4, wherein, in step (B),the -cyclodextrin is added in a weight ratio of 3 to 20 when a weight of microcrystalline cellulose is set to 1.

7. A method of removing cholesterol from a food, the method comprising: (A) a step of filling a column with the cellulose-adipate--cyclodextrin structure of claim 1; and (B) a step of passing a cholesterol-containing food through the column.

8. A method of removing cholesterol from a food, comprising: suspending the cellulose-adipate--cyclodextrin structure of claim 1 in a liquefied food.

9. A method of removing cholesterol from a food, the method comprising: (A) a step of filling a column with the cellulose-adipate--cyclodextrin structure of claim 2; and (B) a step of passing a cholesterol-containing food through the column.

10. A method of removing cholesterol from a food, the method comprising: (A) a step of filling a column with the cellulose-adipate--cyclodextrin structure of claim 3; and (B) a step of passing a cholesterol-containing food through the column.

11. A method of removing cholesterol from a food, comprising: suspending the cellulose-adipate--cyclodextrin structure of claim 2 in a liquefied food.

12. A method of removing cholesterol from a food, comprising: suspending the cellulose-adipate--cyclodextrin structure of claim 3 in a liquefied food.

Description

DESCRIPTION OF DRAWINGS

[0020] FIG. 1 illustrates a method of forming linkages for preparing a cellulose-adipate--cyclodextrin structure.

BEST MODE

[0021] Hereinafter, the present invention will be described in more detail with reference to the following examples. The scope of the present invention is not limited to the following examples and covers modifications of the technical spirit substantially equivalent thereto.

EXAMPLE 1

Preparation of Cellulose-Adipate--Cyclodextrin Structure

[0022] In this example, a process for preparing a cellulose-adipate--cyclodextrin structure is described.

[0023] 100 g of microcrystalline cellulose having a size of 0.5 to 1.0 mm was prepared, and 1L water was added to the prepared microcrystalline cellulose to hydrate. After hydration, 1 M NaOH was added to the hydrated microcrystalline cellulose, and pH was adjusted by adding an alkali while mixing, and the same time, 1L of an aqueous solution containing 5% (w/v) adipic acid was slowly added over 30 minutes. In this process, esterification between the hydroxyl group of microcrystalline cellulose and the carboxyl group of adipic acid occurred, and as a result, an intermediate in which adipic acid is linked to microcrystalline cellulose was generated. -cyclodextrin was dissolved in water at a ratio of 10% (w/v), heated to 70 C. and slowly added to a vessel containing the intermediate produced by the reaction of microcrystalline cellulose with adipic acid with stirring. As a result, a cellulose-adipate--cyclodextrin complex was prepared.

[0024] Since the prepared complex was insoluble, the complex was washed with purified water and harvested to obtain a pure cellulose-adipate--cyclodextrin structure. In addition, since unreactive residues were water soluble and thus completely removed by the washing procedure.

EXPERIMENTAL EXAMPLE 1

Measurement of Effect of Cellulose-Adipate--Cyclodextrin Structure on Removing Cholesterol

[0025] In this experimental example, the effect of the cellulose-adipate--cyclodextrin structure obtained in Example 1 on removing cholesterol is investigated.

[0026] A column filled with the cellulose-adipate--cyclodextrin structure and milk containing 20 mg of cholesterol per 100 g of milk were prepared. The temperature of the column was adjusted to 10 C., and the milk was passed from the bottom to the top of the column at an optimal flow rate of 1 vvh (volume/volume/hour). Cholesterol content contained in the milk passed through the column was measured. Measurement of cholesterol content was carried out using high-performance liquid chromatography coupled with an ELS detector.

[0027] As a result, after passing the milk through the column, cholesterol content was reduced from 20 mg/100 g to 0.7 mg/100 g, i.e., 96.5% of cholesterol was removed. In addition, when the column was continuously reused, it was confirmed that, after 10 times of reuses, the removal efficiency was maintained at about 90%.

EXAMPLE 2

Preparation of Puffed Cellulose-Adipate--Cyclodextrin Structure

[0028] In this example, a process for preparing puffed cellulose-adipate--cyclodextrin structure is described.

[0029] The microcrystalline cellulose used in Example 1 was puffed by heating at 350 C. for 10 minutes to form a high pressure and instantaneously removing the high pressure. Thereafter, the following reaction was carried out in the same manner as described in Example 1 to obtain a puffed cellulose-adipate--cyclodextrin structure.

EXPERIMENTAL EXAMPLE 2

Measurement of Effect of Puffed Cellulose-Adipate--Cyclodextrin Structure on Removing Cholesterol

[0030] In this experimental example, the effect of the puffed cellulose-adipate--cyclodextrin structure obtained in Example 2 on removing cholesterol is investigated.

[0031] The puffed cellulose-adipate--cyclodextrin structure in Example 2 was used, and experiments were carried out in the same method as described in Experimental Example 1. As a result, the efficiency of the puffed cellulose-adipate--cyclodextrin structure on cholesterol removal was 98.4%.

[0032] In addition, the structure filled in a column was reusable, the structure can be reused immediately after removing treated milk and the efficiency of the structure was not lowered even when used up to 5 times.

[0033] Average removal efficiency was about 980.9%. When the structure was reused up to 6 to 10 times, average removal efficiency was 960.6%.

[0034] Accordingly, the structure of the present invention was able to be reused at least 10 times according to requirement for the removal efficiency of cholesterol. In addition, when compared to the cellulose-adipate--cyclodextrin structure in Example 1, the puffed microcrystalline cellulose exhibited higher efficiency for cholesterol removal, and was stable and able to be used continuously.

EXPERIMENTAL EXAMPLE 3

Measurement of Effect of Puffed Cellulose-Adipate--Cyclodextrin Structure on Removing Cholesterol

[0035] 30 g of the puffed cellulose-adipate--cyclodextrin structure described in Example 2 was added to 1 L milk containing 20 mg of cholesterol per 100 g of milk, mixed at 8 C. and 200 rpm for 15 minutes and then, filtered. When the cholesterol content of the filtered milk was measured, 97.3% of cholesterol was removed.

[0036] Accordingly, the structure of the present invention exhibited a cholesterol removing effect even when suspended in milk.

EXPERIMENTAL EXAMPLE 4

Measurement of Effect of Puffed Cellulose-Adipate--Cyclodextrin Structure on Removing Cholesterol

[0037] 50 g of the puffed cellulose-adipate--cyclodextrin structure described in Example 2 was added to a mixture containing 500 g of water and 500 g of separated egg yolks, mixed at 10 C. and 150 rpm for 20 minutes and filtered. Filtered egg yolks were sprayed and dried to obtain egg yolk powder.

[0038] The cholesterol content of the obtained egg yolk powder was 1.1 mg/100 g, and removal efficiency was 98%.

INDUSTRIAL APPLICABILITY

[0039] The present invention relates to a structure for removing cholesterol from foods. The structure of the present invention may be applied to remove cholesterol from foods and prepare foods with low cholesterol contents.