UV-LED Induction of Measurable Biological Endpoints

Abstract

Composition, systems, and methods for the induction of biological endpoints in a cheap contained system. In many embodiments, variation on well numbers on plates and ranging ultraviolet wavelengths produces different and controlled outcomes of the biological endpoint induction. Endpoint induction of this type allows for chemical induction, wherein the system can be turned on and off in short time periods.

Claims

1. A method of inducing measurable biological endpoints, including genetic manipulation by providing light at a wavelength within a range of 220 nanometer to 400 nanometers, wherein light source is a light emitting diode.

2. The method as in claim 1, wherein the light wavelength range is 340 nanometers to 400 nanometers.

3. The method as in claim 1, wherein the light wavelength range is 310 nanometers to 340 nanometers.

4. The method as in claim 1, wherein the light wavelength range is 280 nanometers to 310 nanometers.

5. The method as in claim 1, wherein the light wavelength range is 220 nanometers to 280 nanometers.

6. A system comprising: a light emitting diode component; a specimen well plate component; and an exposure control component.

7. The system as in claim 6, wherein the specimen well plate component is removable.

8. The system as in claim 6, wherein the well plate component is variable in size up to 1536 wells.

9. The method as in claim 6, wherein each well plate contains a different sample in each well.

10. The system as in claim 6, wherein the well plate component are spectrophotometer curvettes.

11. The system as in claim 6, wherein the light emitting diode component emits light in wavelength range 340 nanometers to 400 nanometers.

12. The system as in claim 6, wherein the light emitting diode component emits light in wavelength range 310 nanometers to 340 nanometers.

13. The system as in claim 6, wherein the light emitting diode component emits light in wavelength range 280 nanometers to 310 nanometers.

14. The system as in claim 6, wherein the light emitting diode component emits light in wavelength range 220 nanometers to 280 nanometers.

15. A method of making measurable biological endpoints, including genetic manipulation comprising: providing a sample; adding light from light emitting diode light source; and observing result.

16. The method as in claim 15, further comprising adding a well plate.

17. The method as in claim 16, wherein well plate is removable.

18. The method as in claim 16, wherein well plate is variable in size up to 1536 wells.

19. The method as in claim 16, wherein each well plate contains a different sample in each well.

20. The method as in claim 16, wherein the well plate component are spectrophotometer curvettes.

21. The method as in claim 15, wherein the light emitting diode component emits light in wavelength range 340 nanometers to 400 nanometers.

22. The method as in claim 15, wherein the light emitting diode component emits light in wavelength range 310 nanometers to 340 nanometers.

23. The method as in claim 15, wherein the light emitting diode component emits light in wavelength range 280 nanometers to 310 nanometers.

24. The method as in claim 15, wherein the light emitting diode component emits light in wavelength range 220 nanometers to 280 nanometers.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The various advantages of the embodiments of the present invention will become apparent to one skilled in the art by reading the following specification and appended claims, and by referencing the following drawings, in which:

[0017] FIG. 1 is a pictorial representation of an example of a composition according to an embodiment;

[0018] FIG. 2 is a diagrammatic representation of an example of a system inducing biological endpoints according to an embodiment; and

DETAILED DESCRIPTION

[0019] Turning to FIG. 1 the composite parts in an embodiment include a well plate component 10, a light cone array component 12, and ultraviolet light-emitting diode component 14. These three components may be combined to create the damage induction system 16.

[0020] In another embodiment, the damage induction system 16 may be modified by adding additional wells or reducing number of wells in component 10.

[0021] In many embodiments, the damage induction system 16 acts as an optogenetic approach.

[0022] In many embodiments, the wells 10 might number from one large well to 1536 microwells.

[0023] In one embodiment of the damage induction system 16, the ultraviolet light emitting diode component 14 selected might use nanometer lengths 220 to 400. These wavelengths inducing different measurable endpoints.

[0024] In another embodiment, well plate component 10 might be removable.

[0025] In one embodiment light emitting diode component 14 might modulate the wavelengths used based on an algorithm component 18.

[0026] In one embodiment light cone array component 12, sample well plate component 10, and light emitting diode component 14 combines into a portable damage induction system 16.

[0027] In one embodiment system might include a power source component 20; this component might be standard commercial transformer. This or another system might include a heat sink component 22.

[0028] In one embodiment plate component 10 might be composed of plate-based irradiation spectrophotometer curvettes.

[0029] In one embodiment plate component 10 might be composed of 15 ml and 50 ml tubes.

[0030] Many embodiments might involve the illustrated components being installed into a larger sample analysis system. Where the end result is a damage induction system 16. The components ingredients well plate component 10, light cone array component 12, and light emitting diode component 14 may also be included in a large device, so it is possible that making biological endpoints result in further analysis.

[0031] In one embodiment the system has an onboard cooling component that allows the unit to be placed in a biological incubator to be able to irradiate biological material at temperature so as to not disturb the cell environment.

[0032] One embodiment might utilize an algorithm 28 determining exposure and timing for induction. In some embodiments FIG. 2 could result in modulating wavelengths to produce measurable endpoints. Once the induction system 26 is controlled by an algorithm 28 as it might in one example of the schema, it can result in varied samples 30 for analysis.

[0033] In many embodiments of the induction system 26, samples 30 might be derived from the addition of algorithm 28 and exposure modulation component 24.

[0034] In some embodiments the induction system 26 does not utilize algorithm 28 or exposure modulation component 24. In the schema the system might include only the algorithm 28 in addition with the induction system 26; or alternatively only the exposure modulation component 24 with the induction system 26.

RELATED APPLICATIONS

[0035] The present application claims priority to and benefit from U.S. Provisional Application No. 63/270,892 filed Oct. 22, 2021, the entire contents of each of which are herein incorporated by reference.