Method for Manufacturing Neuraminic Acid Derivatives

Abstract

The present invention provides methods for manufacturing neuraminic acid derivatives.

[Means for solution]

Methods for manufacturing compounds represented by the formula (I):

##STR00001##

[wherein R.sup.1 represents a C.sub.1-C.sub.19 alkyl group], or a pharmacologically acceptable salt thereof, using N-acetylneuraminic acid dihydrate as a starting raw material are provided.

Claims

1. A method of manufacturing a compound of formula (7): ##STR00016## comprising: reacting a compound of formula (6): ##STR00017## with azidotrimethylsilane in the presence of titanium (IV) isopropoxide to crystallize the compound of formula (7) in the resulting reaction solution, adding hydroxycarboxylic acid and then an aqueous sodium nitrite solution to the reaction solution, and isolating the compound of formula (7) from the reaction solution.

2. A method of manufacturing a compound of formula (I): ##STR00018## or a pharmacologically acceptable salt thereof, comprising reacting a compound of formula (6): ##STR00019## with azidotrimethylsilane in the presence of titanium (IV) isopropoxide to crystallize a compound of formula (7): ##STR00020## in a resulting reaction solution, adding hydroxycarboxylic acid and then an aqueous sodium nitrite solution to the reaction solution, reacting the compound of formula (7) with triphenylphosphine and then with a base and water to produce a solution of a compound of formula (8), adding an acid to the compound of formula (8), reacting the compound of formula (8) with a compound of formula (9) to produce a compound of formula (10), heating the compound of formula (10) in water to produce a compound of formula (11), reacting the compound of formula (11) with a compound of formula R.sup.1C(OMe).sub.3 in the presence of an acid to produce a compound of formula (12), reacting the compound of formula (12) with water to produce the compound of formula (I) or a pharmacologically acceptable salt thereof, and isolating the compound of formula (I) from the reaction solution; wherein R.sup.1 represents a C.sub.1-C.sub.19 alkyl group.

3. The method of claim 2, wherein R.sup.1 is a 1-heptyl group.

4. A compound of formula (I), or a pharmacologically acceptable salt thereof, produced by the method of claim 2, wherein the 50% by weight particle diameter as determined by laser diffraction/scattering particle size distribution measurement is 5 M to 15 M, and the 90% by weight particle diameter as determined by laser diffraction/scattering particle size distribution measurement is 15 M to 35 M.

Description

MODE FOR CARRYING OUT THE INVENTION

[0096] In the present invention, neuraminic acid derivatives are produced according to the manufacturing method indicated below.

##STR00013##

##STR00014## ##STR00015##

[0097] R.sup.1 represents a C.sub.1-C.sub.19 alkyl group.

(Step 1)

[0098] Step 1 is a step for producing compound (2) by reacting a known compound (1) with methanol in the presence of an acid and a compound represented by the formula HC(OMe).sub.3 (trimethyl orthoformate).

[0099] There are no limitations on the order in which the acid and trimethyl orthoformate are added. They are preferably added in the order of acid and then trimethyl orthoformate.

[0100] There are no limitations on the acid used provided it can be used in an esterification reaction of a carboxyl group that uses an alcohol, and the acid can be, for example, an organic acid such as acetic acid, propionic acid, trifluoroacetic acid and pentafluoropropionic acid, an organic sulfonic acid such as p-toluenesulfonic acid, camphorsulfonic acid and trifluoromethanesulfonic acid, or an inorganic acid such as hydrogen chloride, hydrogen bromide, hydrogen iodide, phosphoric acid, sulfuric acid and nitric acid, is preferably an inorganic acid, and is most preferably sulfuric acid.

[0101] The reaction temperature is 0 C. to 60 C. and preferably 20 C. to 40 C.

[0102] The reaction time is 30 minutes to 10 hours and preferably 1 hour to 4 hours.

[0103] Triethylamine is preferably added after having produced compound (2).

[0104] In the manufacturing method of the present invention, compound (2) is synthesized in the form of a monohydrate. When water serving as raw material for the hydrate is added to the reaction solution, a reverse reaction of Step 1 can occur due to the presence of the acid added in Step 1. When this reverse reaction occurs, the residual amount of the starting material in the form of compound (1) present in compound (2) increases. By adding triethylamine after having produced compound (2), the reverse reaction caused by the acid can be interrupted or the rate at which the reverse reaction proceeds can be decreased. As a result, compound (2) can be crystallized in a state in which the stability of the solution of compound (2) has improved, thereby improving the content of compound (2) in the resulting crystals.

[0105] The amount of triethylamine added to the reaction solution is 0.01 equivalents to 1.00 equivalent, and preferably 0.01 equivalents to 0.20 equivalents, with respect to compound (1).

[0106] The temperature at which ethyl acetate is added dropwise is 0 C. to 60 C. and preferably 10 C. to 40 C.

[0107] The duration of the dropwise addition is 10 minutes to 10 hours and preferably 30 minutes to 4 hours.

(Step 2)

[0108] Step 2 is a step for producing compound (3) by reacting compound (2) with acetic anhydride in the presence of an acid.

[0109] The acid is preferably added gradually at around room temperature in the presence of compound (2) and acetic anhydride until compound (2) dissolves. This is so that the reaction rate can be controlled.

[0110] There are no limitations on the acid used provided it allows acetylation of the hydroxyl groups at the 1-, 2- and 3-positions of the side chain, formation of a carbon-carbon double bond at the 2- and 3-positions of the tetrahydropyran ring, and formation of an oxazoline ring at the 4- and 5-positions of the tetrahydropyran ring to proceed. The acid can be an organic acid such as acetic acid, propionic acid, trifluoroacetic acid and pentafluoropropionic acid, an organic sulfonic acid such as p-toluenesulfonic acid, camphorsulfonic acid and trifluoromethanesulfonic acid, or an inorganic acid such as hydrogen chloride, hydrogen bromide, hydrogen iodide, phosphoric acid, sulfuric acid and nitric acid, is preferably an inorganic acid, and is most preferably sulfuric acid.

[0111] The solvent used is preferably a hydrocarbon, and preferably toluene.

[0112] The reaction temperature is 20 C. to 100 C. and preferably 20 C. to 60 C.

[0113] The reaction time is preferably 30 minutes to 60 hours and more preferably 1 hour to 20 hours.

[0114] Triethylamine and then aqueous ammonia are preferably added to the reaction solution containing compound (3) as post-treatment following production of compound (3) to neutralize the reaction solution. In a reaction in which the reaction solution is neutralized with aqueous ammonia, the pH of the resulting reaction solution is preferably 6 to 10 and more preferably 7 to 10.

(Step 3)

[0115] Step 3 is a step for producing compound (4) by reacting compound (3) with sodium methoxide.

[0116] The solvent used is preferably methanol.

[0117] The reaction temperature is preferably 20 C. to 70 C. and more preferably 0 C. to 50 C.

[0118] The reaction time is preferably 1 minute to 5 hours and more preferably 5 minutes to 1 hour.

(Step 4)

[0119] Step 4 is a step for producing compound (5) by reacting compound (4) with dimethyl carbonate. Compound (5) is produced in the form of crystals.

[0120] In Step 4, a base can also be used preferably. There are no limitations on the base provided it can be used in a transformation reaction of a 1,2-diol to a cyclic carbonate. It is preferably an alkali metal alkoxide and more preferably sodium methoxide.

[0121] The solvent used is preferably methanol.

[0122] The reaction temperature is preferably 30 C. to 80 C. and more preferably 0 C. to 50 C.

[0123] The reaction time is preferably 30 minutes to 60 hours and more preferably 1 hour to 20 hours.

[0124] In case the purity of the resulting crystals of compound (5) is not sufficiently high, the purity can be increased by purifying with reslurrying in methanol. More specifically, a highly pure compound (5) can be obtained by adding crystals of compound (5) to methanol, heating to 20 C. to 60 C. and stirring for 1 hour, followed by cooling to room temperature, stirring, filtering the precipitated crystals and washing the crystals with methanol.

(Step 5)

[0125] Step 5 is a step for producing compound (6) by reacting compound (5) with dimethyl sulfate in the presence of a base.

[0126] The formation of by-products can be inhibited by controlling the reaction rate between compound (5) and dimethyl sulfate. Namely, since the reaction rate can be efficiently controlled by gradually adding the base to the compound (5) and dimethyl sulfate, the formation of by-products can be inhibited, thereby making this preferable. As a result, the purity of compound (7) obtained by proceeding through the next step, Step 6, can be increased.

[0127] There are no limitations on the base used provided it can be used to alkylate hydroxyl groups, and for example, it can be a base indicated in Step 4, is preferably an alkali metal hydride, and is most preferably sodium hydride.

[0128] The solvent used is preferably an ether, an amide or a mixture thereof, more preferably tetrahydrofuran, N,N-dimethylacetamide or mixture thereof, and most preferably a mixture of tetrahydrofuran and N,N-dimethylacetamide.

[0129] When adding the base to the reaction solution, the reaction temperature is preferably 20 C. to 20 C. and more preferably 15 C. to 15 C.

[0130] As the method used to purify compound (6) following production of compound (6), it is preferable to add a solvent immiscible with water to the reaction solution containing compound (6), wash the mixture with an aqueous sodium hydrogencarbonate solution to separate into an organic layer and aqueous layer, and again wash the resulting organic layer with an aqueous sodium hydrogencarbonate solution.

[0131] The solvent used is preferably toluene.

(Step 6)

[0132] Step 6 is a step for producing compound (7) by reacting compound (6) with azidotrimethylsilane in the presence of titanium (IV) isopropoxide.

[0133] The reaction can be carried out at a lower temperature, and therefore more safely, by reacting compound (6) and azidotrimethylsilane in the presence of titanium (IV) isopropoxide, and the desired compound in the form of compound (7) can be synthesized highly selectively from among stereoisomers formed due to orientation differences in the azide group at the 4-position of the tetrahydropyran ring.

[0134] The solvent used is preferably an aromatic hydrocarbon, an alcohol or a mixture thereof, more preferably 2-propanol, 2-methyl-2-propanol, toluene or a mixture thereof, and most preferably a mixture of 2-methyl-2-propanol and toluene.

[0135] The reaction temperature is preferably 20 C. to 80 C. and more preferably 0 C. to 30 C.

[0136] The reaction time is preferably 1 hour to 100 hours and more preferably 5 hours to 30 hours.

[0137] After having produced compound (7) by reacting compound (6) and azidotrimethylsilane in the presence of titanium (IV) isopropoxide, as reaction post-treatment, it is preferable to add a hydroxycarboxylic acid to the reaction solution and after that add sodium nitrite in the form of an aqueous solution to the reaction solution.

[0138] Although titanium (IV) isopropoxide is a liquid at normal temperatures, when compound (7) is produced by reacting compound (6) with azidotrimethylsilane in the presence thereof, and sodium nitrite is added in the form of an aqueous solution to decompose the residual azidotrimethylsilane, insoluble matter which is hardly soluble, derived from the titanium (IV) isopropoxide is formed. However, the formation of insoluble matter which is hardly soluble, derived from the titanium (IV) isopropoxide can be avoided if a hydroxycarboxylic acid is added to the reaction solution. Consequently, compound (7) can be separated from the titanium (IV) isopropoxide and compounds derived from titanium (IV) isopropoxide simply by filtering, thereby making this preferable. Since the formation of insoluble matter which is hardly soluble, derived from the titanium (IV) isopropoxide can be avoided, a high content of compound (7) can be produced.

[0139] The hydroxycarboxylic acid is, for example, lactic acid, tartaric acid or citric acid, preferably lactic acid or tartaric acid, and more preferably lactic acid.

[0140] The hydroxycarboxylic acid can be used in the L form, D form or DL form.

[0141] The reaction temperature in the reaction in which a hydroxycarboxylic acid is added to the reaction solution is 20 C. to 80 C. and preferably 0 C. to 30 C.

[0142] The reaction time is 10 minutes to 100 hours and preferably 30 minutes to 10 hours.

[0143] The solvent used to wash the resulting crystals of compound (7) is preferably methanol. The use of methanol results in lower likelihood of the crystals becoming colored.

(Step 7)

[0144] Step 7 includes a step for treating compound (7) with triphenylphosphine (Step 7a) and a step for treating the compound obtained in Step 7a with a base and water (Step 7b).

(Step 7a)

[0145] The solvent used is preferably tetrahydrofuran or ethyl acetate and more preferably tetrahydrofuran. The procedure is facilitated by adding compound (7) after having dissolved the triphenylphosphine with a solvent, thereby making this preferable.

[0146] The reaction temperature is preferably 30 C. to 100 C. and more preferably 10 C. to 60 C.

[0147] The reaction time is preferably 30 minutes to 100 hours and more preferably 1 hour to 10 hours.

(Step 7b)

[0148] There are no limitations on the base used provided it allows an ester group hydrolysis reaction and cyclic carbonate group elimination reaction to proceed, and the base is preferably an alkali metal hydroxide, more preferably sodium hydroxide or potassium hydroxide, and particularly preferably sodium hydroxide.

[0149] The solvent used is preferably tetrahydrofuran, methanol or ethanol, and more preferably tetrahydrofuran.

[0150] The acid used to adjust the pH of the reaction mixture to the acidic side is preferably hydrochloric acid.

[0151] The reaction temperature is preferably 30 C. to 100 C. and more preferably 0 C. to 70 C.

[0152] The reaction time is preferably 10 minutes to 20 hours and more preferably 30 minutes to 10 hours.

(Step 8)

[0153] Step 8 is a step for producing compound (10) by reacting compound (8) with N,N-bis(tert-butoxycarbonyl)-1H-pyrazole-1-carboximidamide (Compound (9)).

[0154] Compound (9) can be produced by the method described in Patent Document 3, etc.

[0155] In this step, carbonic acid formed as a by-product in Step 7 carried out prior to Step 8 is preferably released in the form of carbon dioxide gas by first adding an acid to the aqueous solution containing compound (8) obtained after synthesizing compound (8) to adjust the pH of the aqueous solution to the acidic side as previously described.

[0156] The pH of the reaction solution following addition of acid is preferably 1 to 5.

[0157] After removing carbon dioxide gas by addition of acid, the pH of the reaction solution is preferably returned to the alkaline side by adding a base. The pH of the reaction solution following addition of base is preferably 7.5 to 12.0 and more preferably 8.5 to 11.0.

[0158] The acid used to adjust the pH of the reaction mixture to the acidic side is preferably hydrochloric acid.

[0159] The base used to return the pH of the reaction mixture to the alkaline side is preferably sodium hydroxide.

[0160] The solvent used is preferably a mixture of water and an alcohol and more preferably a mixture of water and methanol.

[0161] The reaction temperature is preferably 30 C. to 80 C. and more preferably 0 C. to 50 C.

[0162] The reaction time is preferably 1 hour to 160 hours and more preferably 5 hours to 80 hours.

(Step 9)

[0163] Step 9 is a step for producing compound (11) by heating compound (10) in water.

[0164] The solvent used is preferably water.

[0165] The reaction temperature is preferably 30 C. to 100 C. and more preferably 50 C. to 100 C.

[0166] The reaction time is preferably 30 minutes to 20 hours and more preferably 1 hour to 10 hours.

[0167] Slurry purification with water alone is preferable for increasing the purity of the resulting compound (11).

[0168] Since compound (11) can be produced in the form of crude crystals not having high purity, crystals of compound (11) of high purity are obtained by adding water to the crude crystals, heating and stirring, followed by cooling, filtering out the resulting crystals, washing and drying.

[0169] The temperature during the stirring procedure is preferably 30 C. to 100 C. and more preferably 50 C. to 100 C.

[0170] The duration of stirring is preferably 1 hour to 20 hours and more preferably 2 hours to 10 hours.

[0171] The purity of the resulting compound (11) can be enhanced by recrystallization by adjusting the pH of the reaction solution containing compound (11). Compound (11) can be crystallized by adding an acid such as hydrochloric acid after putting compound (11) into the state of a slurry with methanol containing compound (11) and water and dissolving it, followed by neutralizing with a base such as sodium hydroxide.

[0172] Slurry purification with water alone is more preferable as the method used to purify compound (11).

(Step 10)

[0173] Step 10 is a step for producing compound (12) by reacting compound (11) with a R.sup.1C(OMe).sub.3 [wherein R.sup.1 represents a C.sub.1-C.sub.19 alkyl group] in the presence of an acid.

[0174] The compound represented by the formula R.sup.1C(OMe).sub.3 is preferably 1,1,1-trimethoxyoctane.

[0175] There are no limitations on the acid used provided it allows cyclic orthoesterification reaction of a hydroxyl group using an orthoester to proceed. The acid is preferably an organic sulfonic acid or inorganic acid, more preferably p-toluenesulfonic acid, sulfuric acid or hydrogen chloride, and particularly preferably hydrogen chloride.

[0176] The solvent used is preferably methanol.

[0177] The reaction temperature is preferably 30 C. to 80 C. and more preferably 0 C. to 50 C.

[0178] The reaction time is preferably 5 minutes to 20 hours and more preferably 10 minutes to 5 hours.

(Step 11)

[0179] Step 11 is a step for producing compound (I) by reacting compound (12) with water in the presence of an acid.

[0180] After adding water to the reaction solution containing compound (12) obtained in Step 10 and forming compound (I) by hydrolysis, a base is added to adjust the pH of the reaction solution to 5 to 10 and preferably 6 to 10. Although compound (II) can also be formed together with the forming of compound (I), by adjusting the pH of the reaction solution as described above, compound (I) can be produced with higher selectivity and at a higher yield than compound (II).

[0181] The acid used is preferably hydrochloric acid.

[0182] The base used to adjust the pH of the reaction solution to the basic side is preferably sodium carbonate.

[0183] The reaction temperature is preferably 30 C. to 80 C. and more preferably 0 C. to 50 C.

[0184] The reaction time is preferably 1 minute to 100 hours and more preferably 10 minutes to 5 hours.

[0185] The 50% by weight particle diameter of compound (I) produced according to the manufacturing method of the present invention, and a pharmacologically acceptable salt thereof, as determined by laser diffraction/scattering particle size distribution measurement is 5 M to 15 M, while the 90% by weight particle diameter is 15 M to 35 M.

[0186] Here, laser diffraction/scattering particle size distribution measurement (Particle size analysis. Laser diffraction methods) refers to a method for determining particle size distribution by irradiating a group of particles with laser light and calculating the particle size distribution thereof from the intensity distribution pattern of the diffracted/scattered light emitted therefrom. The measurement method is defined in ISO13320 published by the International Organization for Standardization and is standardized internationally. The particle diameters at 50% and 90% of a weight-based cumulative particle size distribution curve obtained by laser diffraction/scattering particle size distribution measurement are respectively defined as the 50% by weight particle diameter and 90% by weight particle diameter.

[0187] The neuraminic acid derivative (I) according to the present invention is known to have excellent neuraminidase inhibitory activity and is therefore useful as a drug for treatment or prevention of influenza (refer to the aforementioned Patent Document 1 or 2).

[0188] In the case where the neuraminic acid derivative (I) according to the present invention is used as a medicament, especially as a drug for treatment or prevention of influenza, it can be administered orally or parenterally as such, or after mixing with suitable excipients, diluents and the like that are pharmacologically acceptable, and it is preferable that compound (I), which is an active ingredient, is administered in such a manner that it can be directly delivered to the lungs or respiratory tract (including intraoral and intranasal portions).

[0189] These pharmaceutical drugs can be produced by well-known methods using additives such as excipients or diluents.

[0190] Although the dosage amount varies depending on symptoms, weight, age and the like of the subject to be administered (a warm-blooded animal, preferably a human), it is preferable to administer the neuraminic acid derivative (I) as the active ingredient at 5 mg to 120 mg, preferably 20 mg to 80 mg, and specifically, 20 mg, 40 mg or 80 mg per administration in terms of an anhydride depending on weight and age.

EXAMPLES

[0191] The present invention will be described in more detail with reference to the following Examples, Preparation examples and Test examples.

Example 1

Step 1: Methyl N-Acetylneuraminate Monohydrate

[0192] Methanol (450 mL), trimethyl orthoformate (138.3 g) and concentrated sulfuric acid (3.4 g) were added to N-acetylneuraminic acid dihydrate (150 g) at room temperature followed by stirring for 2 hours at 30 C. After cooling the reaction solution to 20 C., triethylamine (1.8 g) and water (53 mL) were added followed by adding ethyl acetate (2100 mL) dropwise over 1 hour and stirring for 1 hour. The suspension was further cooled to 5 C. and stirred for 1 hour at the same temperature followed by filtering the crystals. The crystals were washed with cold ethyl acetate (300 mL) followed by drying under reduced pressure to give the title compound as a white solid (143.3 g, yield: 96.7%).

[0193] MS (FAB): m/z 324 [M+H].sup.+

[0194] HRS (ESI): Exact mass calcd for C.sub.12H.sub.22NO.sub.9 [M+H].sup.+ 324.1295, Found 324.1287.

[0195] IR (KBr): 3494, 3456, 3267, 2904, 2863, 1753, 1621, 1584, 1299, 1158, 1029, 788, 777, 478 cm.sup.1

[0196] .sup.1H NMR (D.sub.2O, 500 MHz): 1.70 (1H, dd, J=11.6, 13.0 Hz), 1.84 (3H, s), 2.10 (1H, dd, J=5.1, 13.0 Hz), 3.34 (1H, dd, J=1.1, 9.3 Hz), 3.41 (1H, dd, J=6.2, 11.9 Hz), 3.52 (1H, ddd, J=2.8, 6.2, 9.3 Hz), 3.62 (1H, dd, J=2.5, 11.9 Hz), 3.63 (3H, s), 3.71 (1H, dd, J=10.2, 10.5 Hz), 3.85 (1H, ddd, J=5.1, 10.2, 11.6 Hz), 3.86 (1H, dd, J=1.1, 10.5 Hz).

[0197] .sup.13C NMR (D.sub.2O, 125 MHz): 22.1, 38.7, 52.1, 53.6, 63.2, 66.7, 68.3, 70.2, 70.4, 95.4, 171.5, 174.9

Example 2

Step 5: Methyl (3aR,4R,7aR)-4-{(S)-methoxy[(4R)-2-oxo-1,3-dioxolan-4-yl]methyl}-2-methyl-3a,7a-dihydro-4H-pyrano[3,4-d][1,3]-oxazole-6-carboxylate

[0198] Tetrahydrofuran (240 mL) and N,N-dimethylacetamide (60 mL) were added to the compound obtained in accordance with Step A-4 of Example 1 described in Patent Document 4 (60 g) and suspended followed by cooling to 5 C. or lower. After adding dimethyl sulfate (31.8 g) to the suspension, 60% sodium hydride (10.2 g) was added gradually followed by stirring for 3 hours at 3 C. Acetic acid (11.5 g) and toluene (540 mL) were then added to the reaction solution followed by washing the mixture with approximately 7% aqueous sodium hydrogencarbonate solution (240 mL) to separate into an organic layer 1 and an aqueous layer 1. The organic layer 1 was washed with approximately 2% aqueous sodium hydrogencarbonate solution (240 mL) to separate into an organic layer 2 and an aqueous layer 2. The aqueous layer 1 was extracted with toluene (180 mL) to separate an organic layer 3, the aqueous layer 2 was extracted with the organic layer 3 to separate an organic layer 4 that was combined with the organic layer 2. The solvent was then distilled off under reduced pressure until the liquid volume of the combined organic layer became 180 mL to give a toluene solution of the title compound.

Step 6: Methyl (2R,3R,4S)-3-acetamide-4-azide-2-{(S)-methoxy[(4R)-2-oxo-1,3-dioxolan-4-yl]methyl}-3,4-dihydro-2H-pyran-6-carboxylate

[0199] After adding 2-methyl-2-propanol (60 mL) to the toluene solution of the compound obtained in Step 5, titanium (IV) isopropoxide (16.3 g) and azidotrimethylsilane (37.5 g) were added followed by stirring the mixture for 15 hours at 18 C. Subsequently, lactic acid (36.2 g) was added and stirred for 1 hour at 20 C. followed by adding water (120 mL) and approximately 19% aqueous sodium nitrite solution (147.8 g) and stirring for 30 minutes at 25 C. The suspension was then cooled to 5 C. or lower followed by stirring for 1 hour at the same temperature and filtering the crystals. After washing the crystals with cold methanol (240 mL), the crystals were dried under reduced pressure to give the title compound as a pale yellow-white solid (62.3 g, yield: 87.9%, stereoisomer ratio: 237).

[0200] MS (FAB): m/z 371 [M+H].sup.+

[0201] HRMS (ESI): Exact mass calcd for C.sub.14H.sub.19N.sub.4O.sub.8 [M+H].sup.+ 371.12029, Found 371.12018.

[0202] IR (KBr): 3314, 2106, 1795, 1731, 1668, 1550, 1379, 1285, 1180, 1075 cm.sup.1

[0203] .sup.1H NMR (DMSO-d6, 500 MHz): 1.89 (3H, s), 3.36 (3H, s), 3.71 (3H, s), 3.88 (1H, dd, J=1.3, 2.0 Hz), 3.99 (1H, ddd, J=8.9, 9.2, 10.6 Hz), 4.20 (1H, dd, J=1.3, 10.6 Hz), 4.29 (1H, dd, J=2.5, 9.2 Hz), 4.54 (1H, dd, J=7.9, 12.2 Hz), 4.56 (1H, dd, J=7.9, 12.2 Hz), 5.06 (1H, ddd, J=2.0, 7.9, 7.9 Hz), 5.81 (1H, d, J=2.5 Hz), 8.16 (1H, d, J=8.9 Hz).

[0204] .sup.13C NMR (DMSO-d6, 125 MHz): 23.4, 47.0, 53.0, 59.0, 61.7, 66.1, 76.7, 77.7, 79.1, 108.6, 144.7, 155.0, 161.7, 170.1.

[0205] The title compound and stereoisomer ratio thereof were measured under the HPLC measurement conditions indicated below.

HPLC Measurement Conditions (1)

[0206] Column: Column packed with 3 m octadecylsilylated silica gel for use in liquid chromatography in a stainless steel tube having an inner diameter of 4.6 mm and length of 25 cm (Cadenza CD-C18, manufactured by Imtakt, 4.6250 mm, 3 m)

[0207] Column temperature: 30 C.

[0208] Measurement wavelength: 210 nm

[0209] Mobile phase A: 0.05 mol/L phosphate buffer solution (pH 3)

[0210] Mobile phase B: Acetonitrile

[0211] Gradient Conditions:

[0212] 0 to 25 min: mobile phase A:mobile phase B=75:25

[0213] 25 to 40 min: mobile phase ratio changed to mobile phase A:mobile phase B=47.5:52.5

[0214] 40 to 65 min: mobile phase A:mobile phase B=47.5:52.5

[0215] [Note, however, that, the 0.05 mol/L phosphate buffer solution (pH 3) of mobile phase A indicates a buffer solution obtained by adding 0.05 mol/L phosphoric acid to a 0.05 mol/L aqueous potassium dihydrogenphosphate solution and adjusting the pH to 3.]

[0216] Flow rate: 0.7 mL/min

[0217] Sample concentration: Approximately 500 g/L

[0218] Injection amount: 5 L

[0219] Retention time of the title compound: Approximately 22 min

[0220] Retention time of the stereoisomer: Approximately 23 min

[0221] Stereoisomer ratio=Peak area of compound (7)/peak area of stereoisomer

Step 7: (2R,3R,4S)-3-acetamide-4-amino-2-[(1R,2R)-2,3-dihydroxy-1-methoxypropyl]-3,4-dihydro-2H-pyran-6-carboxylic acid

[0222] Triphenylphosphine (39.0 g) and tetrahydrofuran (200 mL) were added to the compound obtained in Step 6 (50 g) at room temperature followed by stirring the mixture for 1 hour at 20 C., stirring for 1 hour at 40 C. and cooling to 10 C. or lower. Approximately 10.2% aqueous sodium hydroxide solution (166.4 g) was added to the reaction solution followed by heating to 40 C. and stirring for 2 hours at the same temperature. After cooling the reaction solution to 25 C. or lower, concentrated hydrochloric acid (28.6 g) and ethyl acetate (150 mL) were added followed by allowing to stand undisturbed and then separating the aqueous layer to give an aqueous solution of the title compound.

Step 8: (2R,3R,4S)-3-acetamide-4-[2,3-bis(tert-butoxycarbonyl)guanidino]-2-[(1R,2R)-2,3-dihydroxy-1-methoxypropyl]-3,4-dihydro-2H-pyran-6-carboxylic acid

[0223] After adding concentrated hydrochloric acid to the aqueous solution of the compound obtained in Step 7 to adjust the pH to 2.7 and removing carbon dioxide gas from the system, an aqueous sodium hydroxide solution was added to adjust the pH to 9.5. N,N-Bis(tert-butoxycarbonyl)-1H-pyrazole-1-carboximidamide (46.1 g) and methanol (300 mL) were added to the aqueous solution followed by heating the suspension to 23 C. and stirring for 46 hours at the same temperature. The solvent was distilled off under reduced pressure until the liquid volume of the reaction suspension became approximately 400 mL followed by adding ethyl acetate (165 mL) and distilling off the solvent under reduced pressure to a liquid volume of approximately 400 mL. Ethyl acetate (355 mL) was added to the resulting liquid followed by allowing to stand undisturbed and then separating the aqueous layer, after which ethyl acetate (250 mL) was again added followed by allowing to stand undisturbed and then separating the aqueous layer. Ethyl acetate (350 mL) was added to the resulting aqueous solution, and after adjusting the pH to 2.7 with concentrated hydrochloric acid, the reaction solution was allowed to stand undisturbed to separate into an organic layer 1 and an aqueous layer 1. Ethyl acetate (175 ml) was then added to the aqueous layer 1 followed by allowing to stand undisturbed to separate an organic layer 2. The resulting organic layer 1 and organic layer 2 were combined and the solvent was distilled off under reduced pressure to a liquid volume of approximately 200 mL. Water (150 mL) was then added to the concentrated solution followed by distilling off the solvent under reduced pressure to a liquid volume of approximately 150 mL and adding water (100 mL) to give an aqueous solution of the title compound.

Step 9: (2R,3R,4S)-3-acetamide-2-[(1R,2R)-2,3-dihydroxy-1-methoxypropyl]-4-guanidino-3,4-dihydro-2H-pyran-6-carboxylic acid

[0224] The aqueous solution of the compound obtained in Step 8 was stirred for 4 hours at 80 C. After cooling the reaction solution to 30 C. or lower and adding methanol (500 mL), the mixture was stirred for 1 hour followed by filtering the crystals. After washing the crystals with methanol (100 mL), the crystals were dried under reduced pressure to give the title compound as a white solid (36.3 g, yield: 77.6%). The crude title compound (30 g) was then suspended by adding water (120 mL) followed by heating to 96 C. After stirring the suspension for 3.5 hours at the same temperature, the suspension was cooled to 30 C. or lower followed by adding methanol (90 mL) and stirring for 1 hour. After filtering the suspension and washing the crystals with methanol (60 mL), the crystals were dried under reduced pressure to give the title compound as a white solid (29.1 g, yield: 97.1%).

[0225] MS (FAB): m/z 347[M+H].sup.+

[0226] Anal. calcd for C.sub.l3H.sub.22N.sub.4O.sub.7: C, 45.08; H, 6.40; N, 16.18. Found C, 44.85; H, 6.16; N, 16.09.

[0227] IR (KBr): 3440, 3375, 3256, 1699, 1653, 1587, 1401, 1329, 1284, 1171, 1087, 1029 cm.sup.1

[0228] .sup.1H NMR (D.sub.2O, 500 MHz): 1.94 (3H, s), 3.31 (3H, s), 3.45 (1H, dd, J=1.5, 8.6 Hz), 3.57 (1H, dd, J=5.6, 12.0 Hz), 3.78 (1H, dd, J=3.0, 12.0 Hz), 3.88 (1H, ddd, J=3.0, 5.6, 8.6 Hz), 4.10 (1H, dd, J=9.7, 9.7 Hz), 4.30 (1H, dd, J=1.5, 9.7 Hz), 4.30 (1H, dd, J=2.2, 9.7 Hz), 5.52 (1H, d, J=2.2 Hz).

[0229] .sup.13C NMR (D.sub.2O, 125 MHz): 22.1, 47.7, 51.8, 60.5, 62.5, 69.6, 75.7, 77.8, 104.0, 149.4, 157.0, 169.0, 174.2.

Step 10: (2R,3R,4S)-3-acetamide-4-guanidino-2-{(S)-[(2RS,4R)-2-heptyl-2-methoxy-1,3-dioxolan-4-yl](methoxy)methyl}-3,4-dihydro-2H-pyran-6-carboxylic acid

[0230] Methanol (50 mL), 1,1,1-trimethoxyoctane(trimethyl orthooctanoate) (17.70 g) and 9.2% hydrogen chloride-methanol solution (13.64 g) were added to the compound (10 g) obtained in Step 9 followed by stirring for 1 hour at 25 C. The solvent was distilled off under reduced pressure to a liquid volume of about 35 mL to give a methanol solution of the title compound.

Step 11: (2R,3R,4S)-3-acetamide-4-guanidino-2-[(1R,2R)-2-hydroxy-1-methoxy-3-(octanoyloxy)propyl]-3,4-dihydro-2H-pyran-6-carboxylic acid monohydrate [compound (I)] and (2R,3R,4S)-3-acetamide-4-guanidino-2-[(1S,2R)-3-hydroxy-1-methoxy-2-(octanoyloxy)propyl]-3,4-dihydro-2H-pyran-6-carboxylic acid monohydrate [Compound (II)]

[0231] Water (100 mL) was added to the methanol solution of the compound obtained in Step 10 followed by washing the reaction solution twice with ethyl acetate (50 mL) to separate the aqueous layer. The pH of the reaction solution was adjusted to pH 7.2 with 17% aqueous sodium carbonate solution, and after stirring the reaction solution for 30 minutes, the pH was adjusted to 8.8 with 17% aqueous sodium carbonate solution followed by stirring for 3 hours. Next, the pH of the reaction solution was adjusted to pH 5.3 with concentrated hydrochloric acid followed by cooling to 5 C. or lower, stirring for 1 hour and filtering the crystals. The crystals were then washed with water (50 mL) followed by drying under reduced pressure to give the crude title compound as white crystals (13.59 g, yield: 95.9%). Methanol (60 mL) was then added to the crude title compound (10 g) to dissolve it followed by gradually adding water (120 mL) to the solution at 25 C., cooling to 5 C. or lower, stirring for 1 hour and filtering the crystals. After washing the crystals with 33% aqueous methanol solution (30 mL), the crystals were dried under reduced pressure to give the title compound as white crystals (9.62 g, yield: 96.2%, chemical purity: 99.91%, ratio of compound (I):compound (II)=97:3, particle diameter: 50% by weight particle diameter=8.8 M, 90% by weight particle diameter=25.4 M).

[0232] MS (FAB): m/z 473[M+H].sup.+

[0233] KF moisture content: 3.9%

[0234] Anal. calcd for C.sub.21H.sub.36 N.sub.4O.sub.8 1.065H.sub.2O: C, 51.29; H, 7.82; N, 11.39. Found C, 51.21; H, 7.82; N, 11.32.

[0235] IR (KBr): 3334, 3289, 2929, 1736, 1665, 1640, 1401, 1325, 1283, 1173, 1114 cm.sup.1

[0236] .sup.1H NMR (CD.sub.3OD, 500 MHz): 0.88 (3H, t, J=7.0 Hz), 1.25-1.34 (8H, m), 1.62 (2H, tt, J=7.2, 7.5 Hz), 1.99 (3H, s), 2.35 (2H, t, J=7.5 Hz), 3.38 (3H, s), 3.45 (1H, dd, J=2.5, 8.2 Hz), 4.09-4.14 (2H, m), 4.23 (1H, dd, J=9.0, 9.0 Hz), 4.29-4.36 (3H, m), 5.55 (1H, d, J=2.5 Hz).

[0237] .sup.13C NMR (CD.sub.3OD, 125 MHz): 13.1, 21.5, 22.3, 24.7, 28.8, 28.9, 31.5, 33.7, 47.8, 51.4, 60.0, 65.5, 67.4, 76.1, 78.9, 102.3, 150.3, 157.6, 168.1, 172.2, 174.1

Reference Example 1

Methyl N-acetylneuraminate monohydrate

[0238] Methanol (800 mL), trimethyl orthoformate (37.7 g) and concentrated sulfuric acid (2.5 g) were added to N-acetylneuraminic acid (100 g) at room temperature followed by stirring for 5 hours at 40 C. N,N-Dimethylacetamide (100 mL) was then added to the reaction solution followed by distilling off the solvent under reduced pressure to a liquid volume of 400 mL. After cooling the concentrated solution to 20 C. or lower, water (50 mL) was added and ethyl acetate (1800 mL) was added dropwise over 1 hour followed by stirring for 1 hour. After further cooling the suspension to 5 C., the suspension was stirred for 1 hour at the same temperature followed by filtering the crystals. The crystals were washed with cold ethyl acetate (200 mL) followed by drying under reduced pressure to give the title compound as a white solid (104.8 g, yield: 94.9%).