UREA CYCLE AUGMENTING COMPOSITION AND METHODS OF USE

Abstract

A urea cycle augmenting composition for improving plant growth and yields and quality of crops includes a urea cycle augmenting composition, which comprises two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or salts or derivatives thereof. An amount of the composition applied to a seed or a plant is sufficient to augment the urea cycle of a plant growing from the seed or the plant.

Claims

1. A urea cycle augmenting composition comprising two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or salts or derivatives thereof, wherein an amount of the composition applied to a seed or a plant is sufficient to augment the urea cycle of a plant growing from the seed or the plant.

2. The urea cycle augmenting composition of claim 1, wherein the composition comprises two of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or the salts or the derivatives thereof.

3. The urea cycle augmenting composition of claim 2, wherein the composition comprises one of: L-arginine and L-ornithine, or the salts or the derivatives thereof; L-arginine and agmatine, or the salts or the derivatives thereof; L-arginine and L-aspartic acid, or the salts or derivatives thereof; L-citrulline and L-aspartic acid, or the salts or the derivatives thereof; L-citrulline and L-ornithine, or the salts or the derivatives thereof; L-citrulline and L-arginine, or the salts or the derivatives thereof; L-argininosuccinic acid and L-arginine, or the salts or derivatives thereof; L-argininosuccinic acid and L-aspartic acid, or the salts or derivative thereof; L-argininosuccinic acid and L-ornithine, or the salts or derivatives thereof; L-argininosuccinic acid and L-citrulline, or the salts or derivatives thereof;

4. The urea cycle augmenting composition of claim 1, wherein the composition comprises an aqueous solution of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or the salts or the derivatives thereof.

5. The urea cycle augmenting composition of claim 1, further including the composition comprising one or more of a fertilizer, an adjuvant, an oil, a fungicide, an insecticide, and an herbicide.

6. A method of augmenting the urea cycle in a plant, the method comprising the steps of: providing a urea cycle augmenting composition comprising two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or salts or derivatives thereof; and applying an amount of the composition to one or both of a seed and a plant growing from the seed sufficient to augment the urea cycle of the plant.

7. The method of claim 6, wherein: the composition comprises an aqueous solution of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or the salts or the derivatives thereof; and the composition is applied to one or both of a plurality of seeds and a plurality of plants by one or more of: soaking the seeds, spraying soil proximate to the plants at time of planting of the seeds, spraying soil proximate to the plants during the growing season, spraying foliage of the plants during the growing season, and incorporation of the composition into a fertilizer solution used for fertigation of the plants during the growing season. Incorporation of the composition into or onto a dry fertilizer used for fertilization of the plants during the growing season Injection into the irrigation water and delivered to the crops

8. The method of claim 7, further including an additional step of between one and five additional applications of the composition to the plurality of plants during the growing season.

9. The method of claim 7, wherein composition is applied to the plurality of plants at a rate equivalent to more than 1.0 g of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or the salts or the derivatives thereof, per acre.

10. The method of claim 9, wherein composition is applied to the plurality of plants at a rate equivalent to between 5.0 g and 1000.0 g of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or the salts or the derivatives thereof, per acre.

11. The method of claim 10, wherein composition is applied to the plurality of plants at a rate equivalent to between 10.0 g and 60.0 g of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or the salts or the derivatives thereof, per acre.

12. The composition of claim 1, wherein an amount of the composition applied to the seed or the plant is sufficient to increase one or more of plant biomass, plant nitrogen uptake, quantity of yield from a plant, and quality of yield from the plant growing from the seed or the plant.

Description

BRIEF DESCRIPTION OF SEVERAL VIEWS OF THE DRAWING(S)

[0018] The disclosure will be better understood and objects other than those set forth above will become apparent when consideration is given to the following detailed description thereof. Such description makes reference to the annexed drawings wherein:

[0019] FIG. 1 is a comparative picture view of a control plot and treated plot from a 2019 Pennsylvania Grain Corn Trial.

[0020] FIG. 2 is a comparative picture view of corn roots at the R4 corn growth stage for a control corn plant and for a treated corn plant from the 2019 Pennsylvania Grain Corn Trial.

[0021] FIG. 3 is a flow diagram for a method utilizing an embodiment of the disclosure.

DETAILED DESCRIPTION OF THE INVENTION

[0022] An embodiment of the disclosure meets the needs presented above by generally comprising a urea cycle augmenting composition, which comprises two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or salts or derivatives thereof. An amount of the composition applied to a seed or a plant is sufficient to augment the urea cycle of a plant growing from the seed or the plant.

[0023] The composition generally comprises two of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or the salts or the derivative thereof, although the present invention anticipates the composition comprising three, four, or five of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, L-argininosuccinic acid, and agmatine, or the salts or the derivative thereof.

[0024] The composition may comprise one of L-arginine and L-ornithine, or the salts or the derivative thereof, L-arginine and agmatine, or the salts or the derivative thereof, L-citrulline and L-aspartic acid, or the salts or the derivative thereof, L-ornithine and L-citrulline, or the salts or the derivative thereof, and L-citrulline and L-arginine, or the salts or the derivatives thereof, L-arginine and L-aspartic acid, or the salts or derivatives thereof. L-argininosuccinnic acid and L-arginine, or the salts or the derivatives thereof, L-argininosuccinnic acid and L-aspartic acid, or the salts or the derivatives thereof, L-argininosuccinnic acid and L-ornithine, or the salts or the derivatives thereof, L-argininosuccinnic acid and L-citrulline, or the salts or the derivatives thereof,

[0025] The composition generally comprises an aqueous solution of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, agmatine, and L-argininosuccinic acid or the salts or the derivatives thereof, although the present invention also anticipates the composition being in dry form, such as, but not limited to, powders, granules, pellets, and the like. The present invention also anticipates the composition comprising one or more of a fertilizer, an adjuvant, an oil, a fungicide, an insecticide, an herbicide, and the like.

[0026] In use, the urea cycle augmenting composition enables a method of augmenting the urea cycle in a plant. The method comprises a first step of providing a urea cycle augmenting composition according to the specification above. A second step of the method is applying an amount of the composition to one or both of a seed, annual or perennial plant which is sufficient to augment the urea cycle of the plant.

[0027] The composition may be in the form of an aqueous solution and applied to one or both of a plurality of seeds and a plurality of plants by one or more of soaking the seeds, spraying soil proximate to the plants at time of planting of the seeds, spraying soil proximate to the plants during the growing season, spraying foliage of the plants during the growing season, incorporation of the composition into a fertilizer solution used for fertigation of the plants during the growing season, incorporation of the composition in or or onto dry fertilizer used for fertilization of plants during the growing season or injecting to irrigation or hydroponic solutions. The method of augmenting the urea cycle in a plant may comprise an additional step of a between one and twenty additional applications of the composition to the plurality of plants during the growing season.

[0028] The composition is applied to the plurality of plants at a rate equivalent to more than 1.0 g of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, agmatine, and L-argininosuccinic acid or the salts or the derivative thereof, per acre. The composition may be applied to the plurality of plants at a rate equivalent to between 5.0 g and 1000.0 g of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, agmatine, and L-argininosuccinic acid or the salts or the derivative thereof, per acre. The composition may be applied to the plurality of plants at a rate equivalent to between 10.0 g and 60.0 g of the two or more of L-arginine, L-ornithine, L-aspartic acid, L-citrulline, agmatine, and L-argininosuccinic acid or the salts or the derivative thereof, per acre.

[0029] Provided below are detailed examples of the urea cycle augmenting compositions and methods for their use in greenhouse and field trials. These examples should not be viewed as limiting in regard to compositions, methods, or plant species.

I. Definitions

[0030] CHK stands for check and represents the untreated control for a trial. LSD stands for least significant difference and C.V. stands for Coefficient of Variance. “a” denotes the corresponding value is significantly different from any other value that does not contain the letter “a”. Similarly, “b” denotes the corresponding value is significantly different from any other value that does not contain the letter “b”.

II. Greenhouse Trials

A. Replicated Field Corn Greenhouse Trial

[0031] Table 1 below presents the results of this trial. Corn seeds (n=20) were planted singly in 6-inch pots using commercial potting soil and were allowed to grow to the 3rd collared leaf stage. Fertilizer (30 grams 20-20-20 NPK fertilizer/gallon of water) was applied at 50 mLs to each pot. Plants were watered to maintain consistent moisture levels in the soil. All treatments in Table 1 are listed by weight % in water and were applied at 0.32 ml of which was further diluted in 1 liter of water and was soil applied to the pot in the form of a drench at 50 mL to each pot. The plants were harvested fourteen days after treatment.

[0032] Trial Conclusions: The combination of L-citrulline and potassium aspartate was superior to the combination of L-arginine and L-ornithine in promoting growth of greenhouse corn plants under the conditions of this trial.

TABLE-US-00001 TABLE 1 Treatment Plant Change # Treatment Composition Biomass (g) (g, %) 1 CHK 31.46 b  — 2 11% L-arginine 33.12 ab 1.66 g, 5.3%  2.2% L-ornithine HCl 3 7.4% L-citrulline 36.80 a  5.34 g, 17.0% 7.4% potassium aspartate 4 7.4% 2:1 L-citrulline:DL 35.37 ab 3.91 g, 12.4% malate 7.4% potassium aspartate 5 7.4% L-citrulline 34.88 ab 3.42 g, 10.9% 7.4% L-aspartic acid 6 7.4% 2:1 L-citrulline:DL 33.78 ab 2.32 g, 7.4%  malate 7.4% aspartic acid LSD 5.585  P = 0.05 C.V. 1.531 

B. Replicated Soybean Greenhouse Trial—1

[0033] Table 2 below presents the results of this trial. Soybean seeds (n=20) were planted singly in 6-inch pots using commercial potting soil and were allowed to grow to the 2nd trifoliate stage. Fertilizer (30 grams 20-20-20 NPK fertilizer/gallon of water) was applied at 50 mLs to each pot. Plants were watered to maintain consistent moisture levels in the soil. All treatments in Table 2 are listed by weight % in water and were applied at 0.32 ml of which was further diluted in 1 liter of water and was soil applied to the pot in the form of a drench at 50 mL to each pot. The plants were harvested fourteen days after treatment.

[0034] Trial Conclusions: Combinations of L-citrulline and aspartic acid promoted significant soybean plant growth, regardless of the presence of a counterion or the counterion if a salt form was used in preparing the solution. The combinations of L-citrulline (or salts) and aspartic acid (or salts) were superior to the combination of L-arginine and L-ornithine in promoting growth of greenhouse grown soybean plants under the conditions of this trial.

TABLE-US-00002 TABLE 2 Treatment Plant Change # Treatment Composition Biomass (g) (g, %) 1 CHK 5.63 b — 2 11% L-arginine  5.87 ab 0.24 g, 4.3%  2.2% L-ornithine HCl 3 7.4% L-citrulline 6.54 a 0.91 g, 16.2% 7.4% potassium aspartate 4 7.4% 2:1 L-citrulline:DL 6.82 a 1.19 g, 21.1% malate 7.4% potassium aspartate 5 7.4% L-citrulline 6.92 a 1.29 g, 22.9% 7.4% L-aspartic acid 6 7.4% 2:1 L-citrulline:DL 6.68 a 1.05 g, 18.7% malate 7.4% aspartic acid LSD 5.585  P = 0.05 C.V. 1.531 

C. Replicated Soybean Greenhouse Trial—2

[0035] Table 3 below presents the results of this trial. Soybean seeds (n=20) were planted singly in 6-inch pots using commercial potting soil and were allowed to grow to the 2nd trifoliate stage. Fertilizer (30 grams 20-20-20 NPK fertilizer/gallon of water) was applied at 50 mLs to each pot. Plants were watered to maintain consistent moisture levels in the soil. All treatments were applied as a foliar spray at 0.4 mL per 250 mL of water, until runoff.

[0036] Trial Conclusions: The combination of L-arginine and L-ornithine was superior to the combination of L-citrulline and L-aspartic acid in promoting soybean plant growth.

TABLE-US-00003 TABLE 3 Treatment Treatment Plant Change # Composition Biomass (g) (g, %) 1 CHK 11.7 b — 2 11% L-arginine 16.2 a 4.5 g, 38.4% 2.2% of L-ornithine HCl 3 7.4% L-citrulline 13.3 b 1.6 g, 13.7% 7.4% of L-aspartic acid LSD .sup. 5.585 P = 0.05 C.V. .sup. 1.531

D. 2021 Greenhouse Corn Trial—1

[0037] Table 4 presents the results of this corn trial. Corn seeds (n=20) were planted singly in 6-inch pots using commercial potting soil and were allowed to grow to the 2nd leaf growth stage. Fertilizer (30 grams 20-20-20 NPK fertilizer/gallon of water) was applied at 50 mLs to each pot. Plants were watered to maintain consistent moisture levels in the soil. Treatment 1 is the untreated control. Treatment 2 comprised a 7.4% agmatine sulfate in water, 0.32 mL of which was further diluted to 1 L with water and was soil applied to the pot in the form of a drench at 50 mL per pot.

[0038] Trial Conclusions: Application of agmatine sulfate to plants increased plant biomass, root length, and root tips by 8.79 grams (17.8%), 75.13 cm (5.0%), and 251.00 (3.6%), respectively.

TABLE-US-00004 TABLE 4 Plant Root Trt Biomass length Root # Treatment (g) (cm) Tips 1 CHK 49.26 a 1505.72 a 7061.9 a 2 7.4% agmatine 58.05 b 1580.85 a 7312.9 a sulfate LSD 6.46  195.64   857.96 P = 0.05 C.V. 14.75.sup.   15.52 .sup. 14.62

E. 2021 Greenhouse Corn Trial—2

[0039] Table 5 presents the results of this corn trial. Corn seeds (n=20) were planted singly in 6-inch pots using commercial potting soil and were allowed to grow to the 2nd leaf growth stage. Fertilizer (30 grams 20-20-20 NPK fertilizer/gallon of water) was applied at 50 mLs to each pot. Plants were watered to maintain consistent moisture levels in the soil. All treatments were applied as at 0.32 ml per 1 L of water and was soil applied to the pot in the form of a drench at 50 mL per pot. Treatment 1 is the untreated control. Treatment 2 comprised a 7.4% agmatine sulfate in water, 0.32 mL of which was further diluted to 1 L with water and was soil applied to the pot in the form of a drench at 50 mL per pot. Treatment 3 comprised a 7.4% agmatine sulfate and 7.4% L-arginine in water, 0.32 mL of which was further diluted to 1 L with water and was soil applied to the pot in the form of a drench at 50 mL per pot.

[0040] Trial conclusions: Application of agmatine sulfate alone changed plant biomass, root length, root tips and basal stem diameter by +3.93 g (12.4%), +9.1 cm (1.2%), ˜47.5 (−1.35%), and +0.44 mm (3.9%), respectively. Application of the L-arginine along with the agmatine sulfate changed plant biomass, root length, root tips and basal stem diameter by +3.73 g (11.8%), +123.5 cm (16.4%), +679.8 (19.4%), and +0.53 mm (4.7%), respectively. Both treatments improved desirable plant characteristics, primarily in upper plant biomass, as would be anticipated with increased nitrogen uptake. Interestingly, Treatment 3 promoted increases in other characteristics including root length, number of root tips, and basal stem diameter, all of which allow a plant to explore a larger volume of soil to access to a greater amount of nutrients.

TABLE-US-00005 TABLE 5 Plant Root Basal Biomass Length Root Diameter Trt # Treatment (g) (cm) Tips (mm) 1 CHK 31.68 b 754.18 b 3512.1 a 11.19 b 2 agmatine 35.61 a 763.25 b 3464.6 a 11.63 a Sulfate 3 Arginine/ 35.41 a 877.64 a 4191.9 a 11.72 a agmatine Sulfate LSD 2.60  94.47  712.0  0.43  P = 0.05 C.V. 8.96  13.98  .sup. 20.57 4.37 

III. Field Trials

A. 2020 Nebraska Corn Field Trial.

[0041] Table 6 below presents the results this corn field trial. Corn seed was standard fungicide/insecticide treated. Fertilizer application was uniform for all plots. The plot design for this trial was 4 replications with each replicate being 4 rows by 50 feet long. The arginine/ornithine solution was knifed into the soil of Plot 2 to simulate a “side dress” or root delivery at a rate of 1 pint of the arginine/ornithine per acre (˜52 grams and ˜10.4 grams, respectively, of L-arginine and L-ornithine HCl). The arginine/ornithine solution was applied at the 5th emerged/collared leaf stage.

[0042] Trial conclusions: Treatment with the arginine/ornithine solution at a relatively low dose per acre provided an increase in yield of almost 4%.

TABLE-US-00006 TABLE 6 Yield Yield Plot Treatment (bushels/acre) Change/acre 1 CHK 206.18 a — 2 11% L-arginine 214.33 b 8.15 bushels 2.2% L-ornithine 456.4 lbs HCl (3.95%) LSD .sup. 6.576 P = 0.05 C.V.  1.39

B. 2019 South Dakota Corn Field Trial

[0043] Table 7 below presents the results of this corn field trial. The plot design for this trial was 4 replications with each replicate being 4 rows by 50 feet long. Plot 1 is an untreated control with nitrogen applied at a rate of 175 lbs/acre in the growing season. This is a standard nitrogen fertilizer application rate consistent with grower standard practices in the area. Plot 2 is a second untreated control with nitrogen applied an applied at rate of 220 lbs/acre in the growing season. For Plot 3, nitrogen also applied at a rate of 175 lbs/acre in the growing season. Additionally, 1 pint of the arginine/ornithine solution (˜52 grams and ˜10.4 grams, respectively of L-arginine and L-ornithine HCl) was diluted to 10 gallons with water. The diluted arginine/ornithine solution then was applied to the foliage of the corn plants of Plot 3 at the 5th collared leaf growth stage using an application rate of 10 gallons per acre.

[0044] Trial Conclusions: Arginine/ornithine treatment at a relatively low dose per acre provided an increases in yield of 23.33 bushels per acre (13.0%) and 4.1 bushels per acre (2.06%) for Plot 3 relative to control Plots 1 and 2, respectively. Comparing Plot 3 (arginine/ornithine treated) to Plot 2 (second control), Plot 3 received ˜52 grams and ˜10.4 grams, respectively, of L-arginine and L-ornithine per acre, while Plot 2 had an additional 45 lbs of nitrogen/acre, providing 13.0% and 10.7% increases in yield, respectively, for Plot 3 and Plot 2 relative to Plot 1. This points to substantially increased efficiency in nitrogen uptake and absorption by the plant.

TABLE-US-00007 TABLE 7 Plot Yield # Treatment (bushels/acre) 1 CHK - 1: nitrogen 179.67 b 175 lbs/acre 2 CHK - 2: nitrogen 198.90 a 220 lbs/acre 3 nitrogen 175 lbs/acre 203.00 a 11% L-arginine 2.2% L-ornithine HCl LSD .sup. 8.933 P = 0.05 C.V.  2.03

C. 2020 Michigan Sugar Beet Trial

[0045] Table 8 below presents the results of this sugar beet trial. The plot design for this trial was 6 replications with each replicate being 4 rows by 25 feet long. Plot 1 was an untreated control and Plot 2 was treated with the arginine/ornithine solution at the 2nd true leaf growth stage.

[0046] Trial Conclusions: The application of the Arginine/Ornithine formulation increased yield by 7.18 tons/acre (30.6%), recoverable sugars by 5.1 lbs/ton (2.3%), and overall sugar yield by 1,690.1 lbs/acre (32.2%).

TABLE-US-00008 TABLE 8 Yield Recoverable Sugar Plot (Tons/ Sugar Yield # Treatment Acre) (lbs/ton) (lbs/acre) 1 CHK  23.65 b 220.02 a 5251.7 b 2 11% L-arginine  30.83 a 225.12 a 6941.8 a 2.2% L-ornithine HCl LSD 3.70  7.13  883.46 P = 0.05 C.V. 9.14  2.15  .sup. 9.76

D. 2019 Louisiana Sugarcane Trial

[0047] Table 9 below presents the results of this sugarcane trial, which used a common sugarcane variety (L01-299). The crop was in the 1st ratoon/1st stubble stage meaning that it had been commercially harvested for the 1st time in 2018, also known as plant cane. In early April, Plots 1 and 3 received 120 lbs/acre of nitrogen in the form of UAN 28 (Urea-Ammonium Nitrate) and considered the grower standard. Thereafter, Plot 1 served as an untreated control. For Plot 2, the arginine/ornithine solution was mixed with the UAN (1 pint arginine/ornithine solution/120 lbs UAN), with the resultant mixture then being applied at a rate at 120 lbs/acre of nitrogen to the soil. For Plot 3, approximately 6 weeks after the nitrogen application, the arginine/ornithine solution was applied at 1 pint/acre to the foliage utilizing a high-boom sprayer. Each plot was approximately 1 acre in size and replicated 4 times per treatment.

TABLE-US-00009 TABLE 9 Recover- Sugar Yield able Yield Plot (Tons/ Sugar (lbs/ Fiber # Treatment Acre) (lbs/Ton) acre) (%) 1 CHK 28.96 b 211.5 a 6086.79 b 18.02 a 2 11% L-arginine 34.46 a 224.7 a 7735.76 a 17.48 b (Soil) 2.2% L- ornithine HCl 3 11% L-arginine 35.58 a 214.8 a 7456.56 a 18.54 a (Foliar) 2.2% L- ornithine HCl LSD 4.5.sup.   29.98 957.03  0.56  P = 0.15 C.V. 11.86.sup.   10.34  13.49 2.65 

[0048] Trial Conclusions: Even though the applications were separated by several weeks and different application methodologies, soil verses foliar, the arginine/ornithine solution produced comparable results in terms of increasing total tons per acre and sugar yield per acre.

E. 2020 Nebraska Soybean Trial

[0049] Table 10 below presents the results of this soybean trial. The field was planted at a seeding rate of 150,000 plants per acre as was uniform throughout the trial. The plot design for this trial was 4 replications with each replicate being 4 rows by 30 feet long. In soybeans, nitrogen is not typically applied to the crop due to nitrogen fixation by the air of the symbiotic bacteria, Rhizobium spp. Plot 1 served as an untreated control. Plots 2 and 3 were treated with the arginine/ornithine solution at a rate of 1 pint/acre to the foliage of the plant. The applications were performed at the V3 (3rd trifoliate) and R1 (bloom) growth stages, respectively.

[0050] Trial conclusions: Foliar application of (the arginine/ornithine solution at the V3 and R1 growth stages increased yield by 20.25% and 15.12%, respectively. The large increases in yield are consistent with the arginine/ornithine solution promoting nitrogen fixation by Rhizobium.

TABLE-US-00010 TABLE 10 Plot Yield Change # Treatment (bushels/acre) (bushels/acre) 1 CHK 67.67 b — 2 11% L-arginine 81.37 a 13.70 (V3) 2.2% L-ornithine HCl 3 11% L-arginine  77.90 ab 10.23 (R1) 2.2% L-ornithine HCl LSD 10.52.sup.  P = 0.05 C.V. 6.13 

F. 2020 Michigan Potato Field Trial

[0051] Table 11 below presents the results of this replicated potato field trial. The plot design for this trial was 6 replications with each replicate being 4 rows by 25 feet long. The crop was grown and maintained using grower standard practices for the area. Yield is expressed as hundredweight (cwt) per acre. Plot 1 served as an untreated control and Plot 2 was treated with the arginine/ornithine solution at a rate of 1 pint per acre to the foliage of the plants during bloom.

[0052] Trial conclusions: The application of the arginine/ornithine solution increased desirable yield, US #1 grade, by 57.4 cwt/acre (15.4%) and total yield by 82.7 cwt/acre (18.1%).

TABLE-US-00011 TABLE 11 Yield Yield Total US #1s US #2s Yield Plot (cwt/ (cwt/ (cwt/ # Treatment acre) acre) acre) 1 CHK 373.9 b .sup. 83.8 a 457.7 b 2 11% L-arginine 431.3 a  109.1 a 540.4 a 2.2% L-ornithine HCl LSD 45.0  50.9 26.9  P = 0.05 C.V. .sup. 7.54 35.5 .sup. 3.63

G. 2020 California Almond Field Trial

[0053] Table 12 below presents the results of this almond field trial. The crop was grown and maintained using grower standard practices for the area. The trial was designed as six replications per treatment, each replicate representing one tree. Yield is expressed as lbs/acre and is the meats yield, the saleable portion of the crop. Plot 1 served as an untreated control. Plot 2 was treated four times (March, April, May, and June) with the arginine/ornithine solution at a rate of 1 pint per acre to the foliage of the plants, coincident with fertigation nitrogen applications. Treatments were performed utilizing a high-volume water sprayer delivering 100 gallons per acre.

[0054] Trial conclusions: The sequential application of the arginine/ornithine solution increased saleable yield by 219 lbs/acre.

TABLE-US-00012 TABLE 12 Plot Saleable Yield # Treatment (lbs/acre) 1 CHK 3638.7 b 2 11% L-arginine 3858.2 a 2.2% L-ornithine HCl LSD  130.34 P = 0.05 C.V.  .sup. 2.34

H. 2020 California Table Grape Trial

[0055] Table 13 below presents the results of this table grape trial. The field was grown and maintained using grower standard practices for the area. The trial was conducted as 6 replications per treatment, each replicate representing one vine. Plot 1 served as an untreated control. Plot 2 was treated with the arginine/ornithine solution at 1 quart per acre two times during the berry sizing process.

[0056] Trial conclusion: The application of the arginine/ornithine solution increased the yield by 107.89 boxes per acre (18.2%).

TABLE-US-00013 TABLE 13 Plot Yield # Treatment (Boxes/Acre) 1 CHK 592.97 b 2 11% L-arginine 700.86 a 2.2% L-ornithine HCl LSD P = 0.05 104.65.sup.  C.V.  1.68

I. 2019 Pennsylvania Grain Corn Trial

[0057] Table 14 below presents the results of a grain corn trial. The plot design for this trial was 4 replications with each replicate being 4 rows by 25 feet long. The trial was unique in that the soil utilized for the trial was heavily composted over the previous years and no fertilizer was applied to the trial. Plot 1 served as an untreated control. Plot 2 was treated with the arginine/ornithine solution at 1 quart per acre at the V5 (5th collared leaf) growth stage.

[0058] Trial conclusions: The arginine/ornithine solution increased plant height (inches), stalk girth, and yield while reducing moisture. Shown in FIG. 1 are pictures of Plot 1 (left) and Plot 2 (right) at the R1 growth stage. Of note the improvement in plant height and number of tassels for Plot 2 (treated with arginine/ornithine solution) relative to the untreated plants of Plot 1. Shown in FIG. 2 are pictures of corn roots at the R4 corn growth stage from this trial. For the arginine/ornithine treated plant (left), there is a significant increase in root size and stalk girth comparted to the untreated plant (right).

TABLE-US-00014 TABLE 14 Plant Stalk Moisture Yield Plot Height Girth at Harvest (bu/ # Treatment (in) (in) (%) acre) 1 CHK 109.1 b.sup.  0.919 b 20.06 b 162.7 b 2 11% L-arginine 120.9 a.sup.  1.082 a 18.34 a 198.1 a 2.2% L-ornithine HCl LSD 1.49 0.068.sup.  .sup. 1.0875 31.2  P = 0.10 C.V. 1.58 8.28 .sup.  2.37  .sup. 7.25

[0059] An interesting aspect to this trial was that no synthetic fertilizer was applied, showing the ability of the arginine/ornithine solution increase nitrogen uptake, plant growth and overall yield in high organic matter soils. After noticing a severe yellowing in the cover crop for Plot 2 the following spring (2020), soil samples were analyzed. Table 15 shows the results of this analysis. Of note, the soil nitrate and soil nitrogen levels were reduced in Plot 2 relative to Plot 1. Also of note, the other primary plant nutrients, phosphorus, and potassium, were not dramatically reduced in Plot 2, indicating that nitrogen uptake was the rate limiting step in plant production.

TABLE-US-00015 TABLE 15 Spring Soil Nitrate Nitrogen Phosphorus Potassium Test (2020) (ppm) (%) (ppm) (ppm) Plot 1 44.4 0.26 84 227 Plot 2 4.7 0.22 96 331

J. 2022 Replicated Field Corn Greenhouse Trial

[0060] Table 16 below presents the results of this trial. Corn seeds (n=20) were planted singly in 6-inch pots using commercial potting soil and were allowed to grow to the 3.sup.rd leaf stage. Fertilizer (30 grams 20-20-20 NPK fertilizer/gallon of water) was applied at 50 mLs to each pot. Plants were watered to maintain consistent moisture levels in the soil. All treatments in Table 2 are listed by weight % in water and were applied at 50 mL to each pot. The plants were harvested fourteen days after treatment.

[0061] Trial Conclusions: Various combinations of agmatine, L-aspartic acid, L-arginine, L-citrulline, L-ornithine significantly increased the biomass of corn plants when compared to the untreated check.

TABLE-US-00016 TABLE 16 Treatment Treatment Shoot Mass Change # Composition (Grams) (g/%) 1 CHK 37.1 b 2 11% L-arginine 39.5 a 2.4 g, 6.4% 2.2% L-ornithine HCl 3 7.4% L-arginine 40.0 a 2.8 g, 7.6% 7.4% L-aspartic acid 4 7.4% L-arginine 41.3 a  4.2 g, 11.3% 7.4% L-citrulline 5 7.4% L-aspartic acid 39.6 a 2.5 g, 6.8% 7.4% L-citrulline 6 7.4% L-ornithine HCl 39.9 a 2.7 g, 7.4% 7.4% L-aspartic acid 7 7.4% L-ornithine 40.5 a 3.4 g, 9.2% 7.4% L-citrulline 8 7.4% L-arginine 39.7 a 2.6 g, 6.9% 7.4% agmatine sulfate LSD  1.94 P = 0.10 C.V.  5.01

K. 2021 Nebraska Corn Field Trial.

[0062] Table 17 below presents the results this corn field trial. Corn seed was standard fungicide/insecticide treated. The corn seed was planted at a planting population of 34,000 seeds per acre. Fertilizer application was uniform for all plots. The plot design for this trial was 8 replications with each replicate being 4 rows by 50 feet long. The arginine/ornithine and aspartic/citrulline combinations were applied to the foliage of the corn plants at the 5.sup.th leaf growth stage. The amino acid combinations were sprayed at a rate of 16 fl. oz. per acre with 15 gallons of water as the carrier.

[0063] Trial conclusions: Treatment with the arginine/ornithine and aspartic acid/citrulline increased plant yield by 12.4 bushels per acre and 8.4 bushels per acre, respectively.

TABLE-US-00017 TABLE 17 Yield Yield Plot Treatment (bushels/acre) Change/acre 1 CHK 232.7 b — 2 11% L-arginine 245.2 a 12.5 bushels 2.2% L-ornithine HCl (5.4%) 3 7.4% L-aspartic acid 242.1 a 9.4 bushels 7.4% L-citrulline (4.0%) LSD .sup. 8.63 P = 0.10 C.V. .sup. 4.08

[0064] Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity and understanding, it will be readily apparent, in light of the teachings of this invention, that certain changes and modifications may be made thereto without departing from the spirit or scope of the following claims. For example, the present invention anticipates the L-amino acids being supplied as part of a racemic mixture.