ISOLATED NUCLEIC ACIDS AND QUANTITATIVE TRAIT LOCI (QTL) FROM S. HABROCHAITES AND METHODS OF USE THEREOF FOR INCREASING RESISTANCE TO BACTERIAL SPECK DISEASE IN TOMATO AND OTHER PLANTS
20170107531 ยท 2017-04-20
Inventors
Cpc classification
C12N9/12
CHEMISTRY; METALLURGY
International classification
Abstract
Compositions and methods for increasing disease resistance in plants, particularly tomato plants are disclosed.
Claims
1. A method for producing a plant exhibiting increased pathogen resistance comprising, a) introducing a qRph1 nucleic acid into a recipient plant cell, said nucleic acid encoding at least one protein useful for conferring resistance to at least one plant pathogen, said cell exhibiting increased pathogen resistance when compared to wild type plant cells lacking said qRph1 nucleic acid.
2. The method of claim 1, wherein said plant pathogen is Pseudomonas syringae pv tomato (Pst), and said cell is a tomato plant cell.
3. A transgenic tomato plant produced by the method of claim 1.
4. The method of claim 1, wherein said at least one protein is an RLK encoded by Soly02g072470.
5. A vector comprising an RLK encoded by Soly02g072470.
6. A host cell comprising the vector of claim 5.
7. A plant comprising the cell of claim 6, said plant exhibiting increased resistance to Pst relative to plants lacking said vector.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
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DETAILED DESCRIPTION OF THE INVENTION
[0023] Bacterial speck disease caused by Pseudomonas syringae pv. tomato (Pst) is a persistent problem on tomato. Resistance against race 0 Pst strains is conferred by the Pto protein which recognizes either of two pathogen effectors, AvrPto or AvrPtoB. However, current tomato varieties do not have resistance to the increasingly common race 1 strains which lack these effectors. We identified accessions of Solanum habrochaites that are resistant to the race 1 strain T1. Genome sequence comparisons of T1 and two Pst strains that are virulent on these accessions suggested that known microbe-associated molecular patterns (MAMPs) or effectors are not involved in the resistance. We developed an F2 population from a cross between one T1-resistant accession, LA2109, and a susceptible tomato cultivar to investigate the genetic basis of this resistance. Linkage analysis using whole genome sequence of 58 F2 plants identified quantitative trait loci, qRph1, in a 5.8 Mbp region on chromosome 2, and qRph2, in a 52.4 Mbp region on chromosome 8 which account for 24% and 26% of the phenotypic variability, respectively. High-resolution mapping of qRph1 confirmed it contributed to T1 resistance and delimited it to a 1,060 kbp region containing 139 genes, including three encoding receptor-like proteins and 17 encoding receptor-like protein kinases (RLKs). One RLK gene, Solyc02g072470, is a promising candidate for qRph1 as it is highly expressed in LA2109 and induced upon treatment with MAMPs. Accordingly, qRph1 is useful for enhancing resistance to race 1 strains.
DEFINITIONS
[0024] The following definitions are provided to facilitate an understanding of the present invention:
[0025] qRph1 refers to quantitative trait loci associated with resistance to bacterial speck disease caused by Pseudomonas syringae pv. tomato (Pst). A receptor like kinase (RLK) encoded by Solyc02g072470 is present in the qRph1 region and is highly expressed in LA2109, which is highly resistant to Pst.
[0026] The term qRph1 function is used herein to refer to any qRph1 activity, including without limitation expression levels of qRph1, enzymatic activity, and enhancement of disease resistance.
[0027] An qRph1 homolog is any protein or DNA encoding the same which has similar structural properties (such as sequence identity and folding) to qRph1. An qRph1 ortholog is any protein or DNA encoding the same which has similar structural properties, and similar function (such as expression, enzymatic activity, and binding) to qRph1.
[0028] The term pathogen-inoculated refers to the inoculation of a plant with a pathogen.
[0029] The term disease defense response refers to a change in metabolism, biosynthetic activity or gene expression that enhances a plant's ability to suppress the replication and spread of a microbial pathogen (i.e., to resist the microbial pathogen). Examples of plant disease defense responses include, but are not limited to, production of low molecular weight compounds with antimicrobial activity (referred to as phytoalexins) and induction of expression of defense (or defense-related) genes, whose products include, for example, peroxidases, cell wall proteins, proteinase inhibitors, hydrolytic enzymes, pathogenesis-related (PR) proteins and phytoalexin biosynthetic enzymes, such as phenylalanine ammonia lyase and chalcone synthase. Such defense responses appear to be induced in plants by several signal transduction pathways involving secondary defense signaling molecules produced in plants. Agents that induce disease defense responses in plants include, but are not limited to: (1) microbial pathogens, such as fungi, oomycetes, bacteria and viruses; (2) microbial components and other defense response elicitors, such as proteins and protein fragments, small peptides, -glucans, elicitins, harpins and oligosaccharides; and (3) secondary defense signaling molecules produced by the plant, such as SA, H.sub.2O.sub.2, ethylene, jasmonates, and nitric oxide.
[0030] The terms defense-related genes and defense-related proteins refer to genes or their encoded proteins whose expression or synthesis is associated with or induced after infection with a pathogen to which the plant is usually resistant.
[0031] A transgenic plant refers to a plant whose genome has been altered by the introduction of at least one heterologous nucleic acid molecule.
[0032] Nucleic acid or a nucleic acid molecule as used herein refers to any DNA or RNA molecule, either single or double stranded and, if single stranded, the molecule of its complementary sequence in either linear or circular form. In discussing nucleic acid molecules, a sequence or structure of a particular nucleic acid molecule may be described herein according to the normal convention of providing the sequence in the 5 to 3 direction. With reference to nucleic acids of the invention, the term isolated nucleic acid is sometimes used. This term, when applied to DNA, refers to a DNA molecule that is separated from sequences with which it is immediately contiguous in the naturally occurring genome of the organism in which it originated. For example, an isolated nucleic acid may comprise a DNA molecule inserted into a vector, such as a plasmid or virus vector, or integrated into the genomic DNA of a prokaryotic or eukaryotic cell or host organism.
[0033] When applied to RNA, the term isolated nucleic acid refers primarily to an RNA molecule encoded by an isolated DNA molecule as defined above. Alternatively, the term may refer to an RNA molecule that has been sufficiently separated from other nucleic acids with which it would be associated in its natural state (i.e., in cells or tissues). An isolated nucleic acid (either DNA or RNA) may further represent a molecule produced directly by biological or synthetic means and separated from other components present during its production.
[0034] The terms percent similarity, percent identity and percent homology when referring to a particular sequence are used as set forth in the University of Wisconsin GCG software program.
[0035] The term substantially pure refers to a preparation comprising at least 50 60% by weight of a given material (e.g., nucleic acid, oligonucleotide, protein, etc.). More preferably, the preparation comprises at least 75% by weight, and most preferably 90 95% by weight of the given compound. Purity is measured by methods appropriate for the given compound (e.g. chromatographic methods, agarose or polyacrylamide gel electrophoresis, HPLC analysis, and the like).
[0036] A replicon is any genetic element, for example, a plasmid, cosmid, bacmid, phage or virus, that is capable of replication largely under its own control. A replicon may be either RNA or DNA and may be single or double stranded.
[0037] A vector is any vehicle to which another genetic sequence or element (either DNA or RNA) may be attached so as to bring about the replication of the attached sequence or element.
[0038] An expression operon refers to a nucleic acid segment that may possess transcriptional and translational control sequences, such as promoters, enhancers, translational start signals (e.g., ATG or AUG codons), polyadenylation signals, terminators, and the like, and which facilitate the expression of a polypeptide coding sequence in a host cell or organism.
[0039] The term oligonucleotide, as used herein refers to sequences, primers and probes of the present invention, and is defined as a nucleic acid molecule comprised of two or more ribo- or deoxyribonucleotides, preferably more than three. The exact size of the oligonucleotide will depend on various factors and on the particular application and use of the oligonucleotide.
[0040] The phrase specifically hybridize refers to the association between two single-stranded nucleic acid molecules of sufficiently complementary sequence to permit such hybridization under pre-determined conditions generally used in the art (sometimes termed substantially complementary). In particular, the term refers to hybridization of an oligonucleotide with a substantially complementary sequence contained within a single-stranded DNA or RNA molecule of the invention, to the substantial exclusion of hybridization of the oligonucleotide with single-stranded nucleic acids of non-complementary sequence.
[0041] The term probe as used herein refers to an oligonucleotide, polynucleotide or nucleic acid, either RNA or DNA, whether occurring naturally as in a purified restriction enzyme digest or produced synthetically, which is capable of annealing with or specifically hybridizing to a nucleic acid with sequences complementary to the probe. A probe may be either single-stranded or double-stranded. The exact length of the probe will depend upon many factors, including temperature, source of probe and method of use. For example, for diagnostic applications, depending on the complexity of the target sequence, the oligonucleotide probe typically contains 15-25 or more nucleotides, although it may contain fewer nucleotides. The probes herein are selected to be substantially complementary to different strands of a particular target nucleic acid sequence. This means that the probes must be sufficiently complementary so as to be able to specifically hybridize or anneal with their respective target strands under a set of pre-determined conditions. Therefore, the probe sequence need not reflect the exact complementary sequence of the target. For example, a non-complementary nucleotide fragment may be attached to the 5 or 3 end of the probe, with the remainder of the probe sequence being complementary to the target strand. Alternatively, non-complementary bases or longer sequences can be interspersed into the probe, provided that the probe sequence has sufficient complementarity with the sequence of the target nucleic acid to anneal therewith specifically. The term primer as used herein refers to an oligonucleotide, either RNA or DNA, either single-stranded or double-stranded, either derived from a biological system, generated by restriction enzyme digestion, or produced synthetically which, when placed in the proper environment, is able to functionally act as an initiator of template-dependent nucleic acid synthesis. When presented with an appropriate nucleic acid template, suitable nucleoside triphosphate precursors of nucleic acids, a polymerase enzyme, suitable cofactors and conditions such as appropriate temperature and pH, the primer may be extended at its 3 terminus by the addition of nucleotides by the action of a polymerase or similar activity to yield a primer extension product. The primer may vary in length depending on the particular conditions and requirement of the application. For example, in diagnostic applications, the oligonucleotide primer is typically 15-25 or more nucleotides in length. The primer must be of sufficient complementarity to the desired template to prime the synthesis of the desired extension product, that is, to be able to anneal with the desired template strand in a manner sufficient to provide the 3 hydroxyl moiety of the primer in appropriate juxtaposition for use in the initiation of synthesis by a polymerase or similar enzyme. It is not required that the primer sequence represent an exact complement of the desired template. For example, a non-complementary nucleotide sequence may be attached to the 5 end of an otherwise complementary primer. Alternatively, non-complementary bases may be interspersed within the oligonucleotide primer sequence, provided that the primer sequence has sufficient complementarity with the sequence of the desired template strand to functionally provide a template-primer complex for the synthesis of the extension product.
[0042] Polymerase chain reaction (PCR) has been described in U.S. Pat. Nos. 4,683,195, 4,800,195, and 4,965,188, the entire disclosures of which are incorporated by reference herein.
[0043] The term promoter region refers to the 5 regulatory regions of a gene (e.g., CaMV 35S promoters and/or tetracycline repressor/operator gene promoters).
[0044] As used herein, the terms reporter, reporter system, reporter gene, or reporter gene product shall mean an operative genetic system in which a nucleic acid comprises a gene that encodes a product that when expressed produces a reporter signal that is a readily measurable, e.g., by biological assay, immunoassay, radio immunoassay, or by calorimetric, fluorogenic, chemiluminescent or other methods. The nucleic acid may be either RNA or DNA, linear or circular, single or double stranded, antisense or sense polarity, and is operatively linked to the necessary control elements for the expression of the reporter gene product. The required control elements will vary according to the nature of the reporter system and whether the reporter gene is in the form of DNA or RNA, but may include, but not be limited to, such elements as promoters, enhancers, translational control sequences, poly A addition signals, transcriptional termination signals and the like.
[0045] The terms transform, transfect, transduce, shall refer to any method or means by which a nucleic acid is introduced into a cell or host organism and may be used interchangeably to convey the same meaning. Such methods include, but are not limited to, transfection, electroporation, microinjection, PEG-fusion and the like.
[0046] The introduced nucleic acid may or may not be integrated (covalently linked) into nucleic acid of the recipient cell or organism. In bacterial, yeast, plant and mammalian cells, for example, the introduced nucleic acid may be maintained as an episomal element or independent replicon such as a plasmid. Alternatively, the introduced nucleic acid may become integrated into the nucleic acid of the recipient cell or organism and be stably maintained in that cell or organism and further passed on or inherited to progeny cells or organisms of the recipient cell or organism. Finally, the introduced nucleic acid may exist in the recipient cell or host organism only transiently.
[0047] The term selectable marker gene refers to a gene that when expressed confers a selectable phenotype, such as antibiotic resistance, on a transformed cell or plant.
[0048] The term operably linked means that the regulatory sequences necessary for expression of the coding sequence are placed in the DNA molecule in the appropriate positions relative to the coding sequence so as to effect expression of the coding sequence. This same definition is sometimes applied to the arrangement of transcription units and other transcription control elements (e.g. enhancers) in an expression vector.
[0049] The term DNA construct refers to a genetic sequence used to transform plants and generate progeny transgenic plants. These constructs may be administered to plants in a viral or plasmid vector. Other methods of delivery such as Agrobacterium T-DNA mediated transformation and transformation using the biolistic process are also contemplated to be within the scope of the present invention. The transforming DNA may be prepared according to standard protocols such as those set forth in Current Protocols in Molecular Biology, eds. Frederick M. Ausubel et al., John Wiley & Sons, 1995.
[0050] The term co-suppression refers to a process whereby expression of a gene, which has been transformed into a cell or plant (transgene), causes silencing of the expression of endogenous genes that share sequence identity with the transgene. Silencing of the transgene also occurs.
[0051] Amino acid residues described herein are preferred to be in the L isomeric form. However, residues in the D isomeric form may be substituted for any L-amino acid residue, provided the desired properties of the polypeptide are retained. All amino-acid residue sequences represented herein conform to the conventional left-to-right amino-terminus to carboxy-terminus orientation.
[0052] The term isolated protein or isolated and purified protein is sometimes used herein. This term refers primarily to a protein produced by expression of an isolated nucleic acid molecule of the invention. Alternatively, this term may refer to a protein that has been sufficiently separated from other proteins with which it would naturally be associated, so as to exist in substantially pure form. Isolated is not meant to exclude artificial or synthetic mixtures with other compounds or materials, or the presence of impurities that do not interfere with the fundamental activity, and that may be present, for example, due to incomplete purification, or the addition of stabilizers.
[0053] The present invention also includes active portions, fragments, derivatives and functional or non-functional mimetics of qRph1-related polypeptides, or proteins of the invention. An active portion of such a polypeptide means a peptide that is less than the full length polypeptide, but which retains measurable biological activity.
[0054] A fragment or portion of an qRph1-related polypeptide means a stretch of amino acid residues of at least about five to seven contiguous amino acids, often at least about seven to nine contiguous amino acids, typically at least about nine to thirteen contiguous amino acids and, most preferably, at least about twenty to thirty or more contiguous amino acids. Fragments of the qRph1-related polypeptide sequence, antigenic determinants, or epitopes are useful for eliciting immune responses to a portion of the qRph1-related protein amino acid sequence for the effective production of immunospecific anti-qRph1 antibodies.
[0055] The phrase consisting essentially of when referring to a particular nucleotide or amino acid means a sequence having the properties of a given SEQ ID NO. For example, when used in reference to an amino acid sequence, the phrase includes the sequence per se and molecular modifications that would not affect the basic and novel characteristics of the sequence.
[0056] The term tag, tag sequence or protein tag refers to a chemical moiety, either a nucleotide, oligonucleotide, polynucleotide or an amino acid, peptide or protein or other chemical, that when added to another sequence, provides additional utility or confers useful properties, particularly in the detection or isolation, of that sequence. Thus, for example, a homopolymer nucleic acid sequence or a nucleic acid sequence complementary to a capture oligonucleotide may be added to a primer or probe sequence to facilitate the subsequent isolation of an extension product or hybridized product. In the case of protein tags, histidine residues (e.g., 4 to 8 consecutive histidine residues) may be added to either the amino- or carboxy-terminus of a protein to facilitate protein isolation by chelating metal chromatography. Alternatively, amino acid sequences, peptides, proteins or fusion partners representing epitopes or binding determinants reactive with specific antibody molecules or other molecules (e.g., flag epitope, c-myc epitope, transmembrane epitope of the influenza A virus hemaglutinin protein, protein A, cellulose binding domain, calmodulin binding protein, maltose binding protein, chitin binding domain, glutathione S-transferase, and the like) may be added to proteins to facilitate protein isolation by procedures such as affinity or immunoaffinity chromatography. Chemical tag moieties include such molecules as biotin, which may be added to either nucleic acids or proteins and facilitates isolation or detection by interaction with avidin reagents, and the like. Numerous other tag moieties are known to, and can be envisioned by the trained artisan, and are contemplated to be within the scope of this definition.
[0057] A clone or clonal cell population is a population of cells derived from a single cell or common ancestor by mitosis.
[0058] A cell line is a clone of a primary cell or cell population that is capable of stable growth in vitro for many generations.
[0059] The following description sets forth the general procedures involved in practicing the present invention. To the extent that specific materials are mentioned, it is merely for purposes of illustration and is not intended to limit the invention. Unless otherwise specified, general biochemical and molecular biological procedures, such as those set forth in Sambrook et al., Molecular Cloning, Cold Spring Harbor Laboratory (1989) (hereinafter Sambrook et al.) or Ausubel et al. (eds) Current Protocols in Molecular Biology, John Wiley & Sons (1997) (hereinafter Ausubel et al.) are used.
II. Preparation of qRph1 Encoding Nucleic Acid Molecules:
[0060] Nucleic acid molecules of the invention encoding qRph1 polypeptides may be prepared by two general methods: (1) synthesis from appropriate nucleotide triphosphates, or (2) isolation from biological sources. Both methods utilize protocols well known in the art. The availability of nucleotide sequence information, such as the DNA sequences encoding the proteins present in qRph1, enables preparation of an isolated nucleic acid molecule of the invention by oligonucleotide synthesis. Synthetic oligonucleotides may be prepared by the phosphoramidite method employed in the Applied Biosystems 38A DNA Synthesizer or similar devices. The resultant construct may be used directly or purified according to methods known in the art, such as high performance liquid chromatography (HPLC).
[0061] Specific probes for identifying such sequences as the qRph1 encoding sequence may be between 15 and 40 nucleotides in length. For probes longer than those described above, the additional contiguous nucleotides are provided within the reference sequences disclosed herein.
[0062] Additionally, cDNA or genomic clones having homology with qRph1 may be isolated from other species using oligonucleotide probes corresponding to predetermined sequences within the qRph1 nucleic acids of the invention. Such homologous sequences encoding qRph1 may be identified by using hybridization and washing conditions of appropriate stringency. For example, hybridizations may be performed, according to the method of Sambrook et al., Molecular Cloning, Cold Spring Harbor Laboratory (1989), using a hybridization solution comprising: 5SSC, 5Denhardt's reagent, 1.0% SDS, 100 m/ml denatured, fragmented salmon sperm DNA, 0.05% sodium pyrophosphate and up to 50% formamide. Hybridization is carried out at 37-42 C. for at least six hours. Following hybridization, filters are washed as follows: (1) 5 minutes at room temperature in 2SSC and 1% SDS; (2) 15 minutes at room temperature in 2SSC and 0.1% SDS; (3) 30 minutes 1 hour at 37 C. in 1SSC and 1% SDS; (4) 2 hours at 42-65 C. in 1SSC and 1% SDS, changing the solution every 30 minutes.
[0063] One common formula for calculating the stringency conditions required to achieve hybridization between nucleic acid molecules of a specified sequence homology (Sambrook et al., 1989) is as follows:
T.sub.m=81.5 C.+16.6 Log [Na+]+0.41(% G+C)0.63(% formamide)600/#bp in duplex
[0064] As an illustration of the above formula, using [Na+], [0.368] and 50% formamide, with GC content of 42% and an average probe size of 200 bases, the T., is 57 C. The T., of a DNA duplex decreases by 1-1.5 C. with every 1% decrease in homology. Thus, targets with greater than about 75% sequence identity would be observed using a hybridization temperature of 42 C.
[0065] The stringency of the hybridization and wash depend primarily on the salt concentration and temperature of the solutions. In general, to maximize the rate of annealing of the probe with its target, the hybridization is usually carried out at salt and temperature conditions that are 20-25 C. below the calculated T., of the hybrid. Wash conditions should be as stringent as possible for the degree of identity of the probe for the target. In general, wash conditions are selected to be approximately 12-20 C. below the T., of the hybrid. In regards to the nucleic acids of the current invention, a moderate stringency hybridization is defined as hybridization in 6SSC, 5Denhardt's solution, 0.5% SDS and 100 g/ml denatured salmon sperm DNA at 42 C., and washed in 2SSC and 0.5% SDS at 55 C. for 15 minutes. A high stringency hybridization is defined as hybridization in 6SSC, 5Denhardt's solution, 0.5% SDS and 100 g/ml denatured salmon sperm DNA at 42 C., and washed in 1SSC and 0.5% SDS at 65 C. for 15 minutes. A very high stringency hybridization is defined as hybridization in 6SSC, 5Denhardt's solution, 0.5% SDS and 100 g/ml denatured salmon sperm DNA at 42 C., and washed in 0.1SSC and 0.5% SDS at 65 C. for 15 minutes.
[0066] The nucleic acid molecules described herein include cDNA, genomic DNA, RNA, and fragments thereof which may be single- or double-stranded. Thus, nucleic acids are provided having sequences capable of hybridizing with at least one sequence of a nucleic acid sequence, such as selected segments of the sequences encoding qRph1. Also contemplated in the scope of the present invention are methods of use for oligonucleotide probes which specifically hybridize with the DNA from the sequences encoding qRph1 under high stringency conditions. Primers capable of specifically amplifying the sequences encoding qRph1 are also provided. As mentioned previously, such oligonucleotides are useful as primers for detecting, isolating and amplifying sequences encoding the proteins present on qRph1.
[0067] Also provided in accordance with the present invention are transgenic plants containing the aforementioned qRph1, or fragments or derivatives thereof. Such transgenic plants exhibiting enhanced disease resistance are described in greater detail below.
III. Preparation of qRph1 Proteins and Antibodies:
[0068] The proteins present on qRph1 of the present invention may be prepared in a variety of ways, according to known methods. The protein may be purified from appropriate sources, e.g., plant cells or tissues as described in detail in Example 1. Example 1 describes the isolation of qRph1 from LA2109 and expression of this region in F2 plants.
[0069] Once nucleic acid molecules encoding the proteins on qRph1 have been obtained, the qRph1 protein (e.g., RLK, Solyc02g072470) can be produced using in vitro expression methods known in the art. For example, a cDNA or gene may be cloned into an appropriate in vitro transcription vector, such a pSP64 or pSP65 vector for in vitro transcription, followed by cell-free translation in a suitable cell-free translation system, such as wheat germ or rabbit reticulocytes. In vitro transcription and translation systems are commercially available, e.g., from Promega Biotech, Madison, Wis. or BRL, Rockville, Md.
[0070] According to a preferred embodiment, larger quantities of the RLK on qRph1 may be produced by expression in a suitable procaryotic or eucaryotic system. For example, part or all of a DNA molecule may be inserted into a plasmid vector adapted for expression in a bacterial cell (such as E. coli) or a yeast cell (such as Saccharomyces cerevisiae), or into a baculovirus vector for expression in an insect cell. Such vectors comprise the regulatory elements necessary for expression of the DNA in the host cell, positioned in such a manner as to permit expression of the DNA in the host cell. Such regulatory elements required for expression include promoter sequences, translation control sequences and, optionally, enhancer sequences.
[0071] The qRph1 associated RLK protein produced by gene expression in a recombinant procaryotic or eucaryotic system may be purified according to methods known in the art. In a preferred embodiment, the recombinant protein contains several (e.g., 6 8) histidine residues on the amino or carboxyl termini, which allows the protein to be affinity purified on a nickel column. If histidine tag-vectors are not used, an alternative approach involves purifying the recombinant protein by affinity separation, such as by immunological interaction with antibodies that bind specifically to the recombinant protein. Such methods are commonly used by skilled practitioners.
[0072] qRph1 proteins, prepared by the aforementioned methods, may be analyzed according to standard procedures. Methods for analyzing the physical characteristics and biological activity of qRph1 are set forth in U.S. Pat. No. 6,136,552, the disclosure of which is incorporated by reference herein.
[0073] The present invention also provides antibodies capable of immunospecifically binding to proteins of the invention. Polyclonal or monoclonal antibodies directed toward the proteins encoded by the qRph1 region may be prepared according to standard methods. Monoclonal antibodies may be prepared according to general methods of Kohler and Milstein, following standard protocols. In a preferred embodiment, antibodies are prepared, which react immunospecifically with various epitopes of such proteins.
IV. Uses of qRph1 Nucleic Acid Molecules and Proteins:
[0074] A. Nucleic Acids Encoding qRph1-Related Proteins
[0075] Nucleic acids encoding qRph1 proteins may be used for a variety of purposes in accordance with the present invention. DNA, RNA, or fragments thereof encoding qRph1 proteins may be used as probes to detect the presence of and/or expression of such genes. Methods in which nucleic acids encoding qRph1 proteins may be utilized as probes for such assays include, but are not limited to: (1) in situ hybridization; (2) Southern hybridization (3) Northern hybridization; and (4) assorted amplification reactions such as polymerase chain reactions (PCR).
[0076] The nucleic acids of the invention may also be utilized as probes to identify related genes from other plant species, animals and microbes. As is well known in the art, hybridization stringencies may be adjusted to allow hybridization of nucleic acid probes with complementary sequences of varying degrees of homology. Thus, nucleic acids encoding qRph1 proteins may be used to advantage to identify and characterize other genes of varying degrees of relation to the genes of the invention thereby enabling further characterization of the molecular mechanisms disease resistance in tomato. Additionally, the nucleic acids of the invention may be used to identify genes encoding proteins that interact with qRph1 proteins (e.g., by the interaction trap technique), which should further accelerate identification of the molecular components involved in the disease defense response. Nucleic acid molecules, or fragments thereof, encoding qRph1 genes, for example, may also be utilized to control the production of qRph1 proteins, thereby regulating the amount of protein available to participate in the induction or maintenance of disease resistance in plants. As mentioned above, antisense oligonucleotides corresponding to essential processing sites in qRph1-related mRNA molecules or other gene silencing approaches may be utilized to inhibit qRph1 protein production in targeted cells. Yet another approach entails the use of double-stranded RNA mediated gene silencing. Alterations in the physiological amount of qRph1 proteins may dramatically affect the activity of other protein factors involved in the induction or maintenance of disease resistance.
[0077] The nucleic acid molecules of the invention may also be used to advantage to identify mutations in qRph1 encoding nucleic acids from plant samples. Nucleic acids may be isolated from plant samples and contacted with the sequences of the invention under conditions where hybridization occurs between sequences of sufficient complementarity. Such duplexes may then be assessed for the presence of mismatched DNA. Mismatches may be due to the presence of a point mutation, insertion or deletion of nucleotide molecules. Detection of such mismatches may be performed using methods well known to those of skill in the art.
[0078] Nucleic acids encoding the qRph1 proteins of the invention may also be introduced into host cells. In a preferred embodiment, plant cells are provided which comprise an qRph1 protein(s) encoding nucleic acid such as RLK on Solyc02g072470 or a variant thereof. Host cells contemplated for use include, but are not limited to, tomato, maize, wheat, rice, potato, barley, canola, bacteria, yeast, and other plant cells. The nucleic acids may be operably linked to appropriate regulatory expression elements suitable for the particular host cell to be utilized. Methods for introducing nucleic acids into host cells are well known in the art. Such methods include, but are not limited to, transfection, transformation, calcium phosphate precipitation, electroporation, lipofection and biolistic methods.
[0079] The host cells described above or extracts prepared from them containing qRph1 may be used as screening tools to identify compounds which modulate qRph1 protein function. Modulation of qRph1 activity, for example, may be assessed by measuring alterations in qRph1-disease resistance activity, qRph1 enzymatic activities, or qRph1 expression levels in the presence and absence of a test compound. Test compounds may also be assessed for the induction and/or suppression of expression of genes regulated by qRph1 proteins.
[0080] The availability of qRph1 protein encoding nucleic acids enables the production of plant species carrying part or all of an qRph1-related gene or mutated sequences thereof, in single or amplified copies. Transgenic plants comprising any one of the qRph1-related sequences described herein are contemplated for use in the present invention. Such plants provide an in vivo model for examining resistance to Pst and may be particularly useful in elucidating the molecular mechanisms that modulate Pst defense responses.
[0081] In another embodiment of the invention, transgenic plants are provided that have enhanced disease resistance. Such transgenic plants may have altered qRph1 activity due to the overexpression or underexpression of qRph1-related genes.
[0082] As described above, the qRph1-related protein-encoding nucleic acids are also used to advantage to produce large quantities of substantially pure proteins, or selected portions thereof.
[0083] The following materials and methods are provided to facilitate the practice of the present invention.
[0084] Wild tomato accessions were obtained from the Tomato Genetics Resource Center (tgrc.ucdavis.edu). The wild tomato accessions, F1, and F2 plants generated from a cross between LA2109 and Rio Grande (RG)-PtoS were grown in Sunshine MVP soil mix (Sun Gro Horticulture, Agawam, Mass.). RG-PtoS and RG-PtoR plants were grown in Cornell Plus mix, consisting of 5.7 cu ft peat moss, 12 cu ft vermiculite, 5 lbs lime, 5 lbs Osmocote Plus 15-9-12 and 1 lb 3 oz Uni-Mix 11-5-11 (Everris, Israeli Chemicals Ltd). Plants were grown in the greenhouse and moved to a growth chamber with 25 C., 75% humidity, and 16-hour light after pathogen inoculation.
MAMP Treatments, Pathogen Inoculation, and Bacterial Growth Assays
[0085] Two lateral leaflets on the second leaf of 3-week old tomato plants were syringe-infiltrated with 1 uM FlgII-28 (ESTNILQRMRELAVQSRNDSNSATDREA) (SEQ ID NO. 1) (Cal, et al., 2011) or 10 uM Csp22 (AVGTVKWFNAEKGFGFITPDDG) (SEQ ID NO. 2) (Felix and Boller, 2003) and harvested 6 hrs later. Tissue samples were immediately frozen in liquid nitrogen and kept at 80 C. until use. Six-week-old plants were inoculated with 10.sup.5 colony-forming units (CFU)/mL P. syringae pv. tomato T1 by vacuum infiltration for the visual monitoring of disease symptoms and 10.sup.4 CFU/mL for the measurement of bacterial populations. For each replicate, three leaf discs were collected and combined; each experiment involved replicates from three independent plants.
Preparation of DNA Libraries and Illumina Sequencing
[0086] Genomic DNA was extracted using a DNeasy Plant Mini kit (Qiagen) and used to make individual libraries as described previously (Zhong et al., 2011). Briefly, 700 ng of DNA from each plant was fragmented using NEBNext dsDNA Fragmentase (New England Biolabs) for 25 minutes at 37 C. before stopping the reaction with EDTA at a final concentration of 125 mM. Genomic DNA enriched for fragments between 300 to 500 bp was purified using AMPure XP beads (Beckman Coulter) and eluted in water. End-repair, dA-tailing, Y-shape adaptor ligation, triple-SPRI purification and size selection, and PCR enrichment were performed as described (Thong et al., 2011). A total of 60 uniquely barcoded libraries was developed (22 for resistant plants, 8 for moderately-resistant plants, 28 for susceptible plants, and two for RG-PtoS) and subdivided into four pools of 15 libraries. Each of the four pools was sequenced in one lane by the Genomics Resources core facility at Weill Cornell Medical College using Illumina HiSeq 2500 paired-end sequencing (2100 bp) technology. LA2109 was sequenced separately in one lane using Illumina HiSeq 2000 paired-end sequencing (2100 bp). All genome sequence data (fastq files) are available in the Genbank SRA (Leinonen et al., 2011) as Bioproject ID: PRJNA289640.
Genotyping and Linkage Analysis
[0087] Illumina sequence reads from F2 plants, LA2109, and PtoS were mapped to the S. lycopersicum reference genome Heinz 1706 SL2.40 (Tomato Genome Consortium, 2012) with Bowtie 2 (Langmead and Salzberg, 2012). Duplicate reads were removed with Picard (http://picard.sourceforge.net) and local realignment around indels was performed using GATK (DePristo et al., 2011). The alignments were converted to mpileup format and SNPs called with SAMtools and bcftools respectively (Li 2011, Li et al., 2009). Biallelic SNPs unique to the LA2109 parent were selected as markers. SNPs at Hardy-Weinberg equilibrium with a minimum read depth of 2, maximum read depth of 10, genotype quality score at least 10, and a quality of at least 950 were selected for further analysis using vcftools (Danecek et al., 2011). A Perl script, snp_window.pl (https://github.com/srs218/manuscripts) was developed to bin the SNPs into windows of 100,000 bp with at least 10 SNPs per window and to calculate a consensus genotype per individual per bin to result in 1,852 markers. An additional 2,681 markers were generated in 1 kb bins with at least 5 SNPs per bin around the linked region on chromosome 2. Missing genotypes were inferred by using the no double-crossover method in r/QTL (Broman et al., 2003). A linkage map was constructed using the marker physical location from S. lycopersicum Heinz 1706 SL2.40 (Tomato Genome Consortium, 2012). A single QTL genome scan using extended Haley-Knott regression was performed to identify candidate QTL loci. A two-dimensional, 2-QTL scan was performed to determine interaction and heritability. All QTL computations were performed using rQTL software (Broman et al., 2003).
High Resolution Mapping with Molecular Markers
[0088] Molecular markers in the qRph1 region were generated on the basis of sequence gaps in the LA2109 genome by comparison with the reference Heinz 1706 reference genome sequence SL2.40. These gaps represent potential DNA insertions or deletions in the LA2109 genome. Primers were designed to span these gap regions and to amplify a fragment from both LA2109 and RG-PtoS genomic DNA. DNA from two young leaves of each resistant F2 plant was isolated using an extraction buffer (200 mM Tris-HCl pH 8.0, 250 mM NaCl, 25 mM EDTA pH 8.0, 0.5% w/v SDS) and dissolved in 100 l distilled water. 1 l DNA was used for PCR amplification. All resistant plants were genotyped with 11 indel molecular markers and plants with a recombination event in the candidate region were used as informative recombinants to delimit the region conferring T1 resistance.
RNA Extraction, Reverse Transcription PCR and Quantitative RT-PCR
[0089] Leaf tissue was ground in liquid nitrogen and total RNA was extracted with TRIzol reagent following the manufacturer's instructions (Life Technologies, cat. #15596-018). The concentration of total RNA was determined with a Nanodrop spectrophotometer (Thermo Scientific Co.). RNA (lug) was treated with TURBO DNA-free Kit (Life Technologies, cat. AM1907M) and tested for DNA contamination by amplifying SlCBL1 (Solyc12g015870) for 25 cycles; no PCR product was detected. The RNA was then used for reverse transcription PCR (RT-PCR). First strand cDNA was synthesized following instructions provided with the RevertAid First Strand cDNA Synthesis kit (Thermo Scientific Co., cat. #K1622). 1 l and 0.4 l of cDNA templates were used for RT-PCR and quantitative-RT-PCR (qRT-PCR), respectively. SlCBL1 was used as an internal control for RT-PCR and as a reference gene for qRT-PCR data normalization (Pombo et al., 2014).
Phylogenetic Analysis
[0090] Tomato genes similar to the qRph1 candidate Solyc02g072470 at the nucleotide and amino acid levels were identified from a BLAST search of the Heinz ITAG2.3 gene predictions (Tomato Genome Consortium, 2012) and used for phylogenetic analyses along with Solyc02g072470, Solyc02g070890 (SlFLS2.1) and Solyc02g070910 (SlFLS2.2). PhyML in SeaView software was used for all analyses with one hundred bootstraps under default settings of the GTR model for nucleotide analysis and the JTT model for amino acid analysis (Gouy et al, 2010). Phylogenetic trees were optimized by FigTree software (http://tree.bio.ed.ac.uk/software/figtree/).
[0091] The following example is provided to illustrate certain embodiments of the invention. It is not intended to limit the invention in any way.
Example I
Solanum habrochaites Accession LA2109 Exhibits Resistance to Pseudomonas syringae pv. tomato Race 1 Strain T1
[0092] In an initial screen of germplasm from the Tomato Genetics Resource Center core collection we discovered that Solanum habrochaites accession LA2109 developed no symptoms of bacterial speck disease after inoculation with the P. s. pv. tomato (Pst) race 1 strain T1 (
TABLE-US-00001 TABLE 1 S. habrochaites accessions that were screened for resistance to Pseudomonas syringae pv. tomato T1. S. habrochaites Disease accession.sup.1 Collection site, region and country index.sup.2 LA0094 Canta-Yangas, Lima, Peru 3 LA0386 Cajamarca, Cajamarca, Peru 3 LA1223 Alausi, Chimborazo, Ecuador 3 LA1253 Pueblo Nuevo-Landangue, Loja, Ecuador 1 LA1255 Loja (Predestal district), Loja, Ecuador 1 LA1266 Pallatanga, Chimborazo, Ecuador 1 LA1353 Contumaza, Cajamarca, Peru 3 LA1361 Pariacoto, Ancash, Peru 2 LA1681 Mushhka, Lima, Peru 3 LA1691 Yauyos, Lima, Peru 3 LA1695 Cacachhuasiin, Cacra, Lima, Peru 3 LA1721 Ticrapo Viejo, Huancavelica, Peru 3 LA1775 Rio Casma, Ancash, Peru 3 LA1778 Rio Casma, Ancash, Peru 2 LA1928 Ocana, Ayacucho, Peru 3 LA2098 Sabiango, Loja, Ecuador 1 LA2100 Sozorango, Loja, Ecuador 2 LA2101 Cariamanga, Loja, Ecuador 3 LA2103 Lansaca, Loja, Ecuador 1 LA2104 Pena Negra, Loja, Ecuador 3 LA2105 Jardin Botanico, Loja, Loja, Ecuador 1 LA2106 Yambra, Loja, Ecuador 1 LA2107 Los Loris, Loja, Ecuador 1 LA2108 Portete de Anganuma, Loja, Ecuador 1 LA2109 Yangana #1, Loja, Ecuador 1 LA2110 Yangana #2, Loja, Ecuador 1 LA2114 San Juan, Loja, Ecuador 1 LA2115 Pucala, Loja, Ecuador 1 LA2128 Zumbi, Zamor-Chinchipe, Ecuador 2 LA2144 Chanchan, Chimborazo, Ecuador 2 LA2409 Miraflores, Lima, Peru 3 LA2859 Yangana, Loja, Ecuador 3 LA2860 Cariamanga, Loja, Ecuador 2 LA2861 Las Juntas, Loja, Ecuador 1 LA2864 Sozorango, Loja, Ecuador 3 LA2869 Matola-LA Toma, Loja, Ecuador 1 .sup.1Accessions were obtained from the Tomato Genetics Resource Center (tgrc.ucdavis.edu) .sup.2Disease index is described in the Results section. Index ranges from 1 for no symptoms of bacterial speck disease to 3 for extensive symptoms of the disease.
TABLE-US-00002 TABLE 2 Geographical coordinates.sup.1 of the collections sites for the Solanum habrochaites accessions that were screened. S. habrochaites accession Latitude Longitude LA0094 11310S; decimal: 11.516667 76410W; decimal: 76.683333 LA0386 7936S; decimal: 7.160000 78300W; decimal: 78.500000 LA1223 21145S; decimal: 2.195900 78512W; decimal: 78.850600 LA1253 4824S; decimal: 4.140000 79120W; decimal: 79.200000 LA1255 35950S; decimal: 3.997222 791236W; decimal: 79.210000 LA1266 2040S; decimal: 2.011111 785830W; decimal: 78.975000 LA1353 7220S; decimal: 7.366667 78480W; decimal: 78.800000 LA1361 9330S; decimal: 9.550000 775330W; decimal: 77.891667 LA1681 12450S; decimal: 12.750000 75500W; decimal: 75.833333 LA1691 122735S; decimal: 12.459722 75555W; decimal: 75.918056 LA1695 124845S; decimal: 12.812500 75471W; decimal: 75.783611 LA1721 13267S; decimal: 13.435278 752755W; decimal: 75.465278 LA1775 9310S; decimal: 9.516667 77530W; decimal: 77.883333 LA1778 933S; decimal: 9.557400 7742W; decimal: 77.715000 LA1928 142355S; decimal: 14.398611 744922W; decimal: 74.822778 LA2098 42157S; decimal: 4.365833 794846W; decimal: 79.812778 LA2100 42136S; decimal: 4.360000 794747W; decimal: 79.796389 LA2101 41956S; decimal: 4.332222 793345W; decimal: 79.562500 LA2103 414S; decimal: 4.233333 7927W; decimal: 79.450000 LA2104 41341S; decimal: 4.228056 792416W; decimal: 79.404444 LA2105 423S; decimal: 4.034167 791156W; decimal: 79.198889 LA2106 412S; decimal: 4.203333 7913W; decimal: 79.230000 LA2107 411S; decimal: 4.183333 7913W; decimal: 79.216667 LA2108 42425S; decimal: 4.406944 79933W; decimal: 79.159167 LA2109 4228S; decimal: 4.368889 791035W; decimal: 79.176389 LA2110 4221S; decimal: 4.366944 791031W; decimal: 79.175278 LA2114 3560S; decimal: 3.933333 791330W; decimal: 79.225000 LA2115 35029S; decimal: 3.841389 791243W; decimal: 79.211944 LA2128 35337S; decimal: 3.893600 784649W; decimal: 78.780200 LA2144 21724S; decimal: 2.290000 785857W; decimal: 78.982500 LA2409 121722S; decimal: 12.289444 754827W; decimal: 75.807500 LA2859 42147S; decimal: 4.363000 791029W; decimal: 79.174600 LA2860 4200S; decimal: 4.333333 79330W; decimal: 79.550000 LA2861 3490S; decimal: 3.816667 79160W; decimal: 79.266667 LA2864 4200S; decimal: 4.333333 79470W; decimal: 79.783333 LA2869 47S; decimal: 4.16667 7922W; decimal: 79.366667 .sup.1Geographical coordinates were obtained from the Tomato Genetics Resource Center (tgrc.ucdavis.edu).
LA2109 has Both Pto-Dependent and Pto-Independent Resistance to P. syringae pv. tomato
[0093] Pto-mediated resistance, which involves recognition of the effector proteins AvrPto or AvrPtoB, has been previously reported in S. habrochaites, so we tested if LA2109 also carries this gene (Riely and Martin, 2001). Upon inoculation of LA2109 with Pst DC3000, a strain which carries both avrPto and avrPtoB, we observed no signs of bacterial speck disease (
Resistance in LA2109 to P. syringae pv. tomato T1 does not Involve AvrPtoB or Pto
[0094] Pst T1 does not have avrPto and although it does have an avrPtoB homolog, the corresponding protein does not appear to accumulate and the strain is therefore not recognized by Pto (Kunkeaw et al., 2010, Lin et al., 2006). Nevertheless we wished to examine the possibility that LA2109 detects AvrPtoB in T1 thus explaining its resistance to this strain. A T1 strain carrying a deletion in avrPtoB is not available so we relied instead on a T1-like strain Pst19 which also has an avrPtoB gene that does not appear to be expressed; importantly an avrPtoB mutant is available for Pst19 (Kunkeaw et al., 2010). Inoculation of LA2109 with Pst19 or Pst19avrPtoB resulted in similar symptoms of speck disease (
LA2109 Response to Known Peptide MAMPs, Type III Effectors, or Coronatine does not Appear to Play a Role in its Resistance to T1
[0095] The available genome sequences of T1, NY-T1, and DC3000 allowed us to investigate whether specific features unique to T1 might explain LA2109 resistance to this strain. The Flg22 region is identical in all three Pst strains as are the Csp22 regions in all six of the cold shock proteins (
[0096] The FlgII-28 region in NY-T1 flagellin has a phenylalanine at position 16 whereas T1 has a serine (Jones et al., 2015). This substitution is known to compromise FlgII-28 PTI-elicitation activity and it could possibly explain the hyper-virulence of NY-T1 (Cai et al., 2011). However, the FlgII-28 region in strains T1 and DC3000 differs by just two amino acids and synthetic peptides of both are fully active in eliciting PTI (
[0097] We next considered the possibility that a specific type III effector in T1 is recognized by LA2109 thereby activating ETI. However, the repertoires of effectors in T1 and NY-T1 were reported recently to be identical except that NY-T1 also has an effector candidate with limited similarity to HopAO1 (Jones et al., 2015). It is possible that NY-T1 evades ETI because HopAO1 interferes with the recognition of an effector by LA2109. To test this possibility we cloned the HopAO1 candidate effector gene from NY-T1 and transformed it into T1. Inoculation of the T1(hopAO1) strain onto LA2109 revealed no change in T1 virulence (
[0098] Finally, we considered whether LA2109 might respond differently to coronatine as NY-T1 and DC3000 produce this phytotoxin, but T1 does not (Almeida et al., 2009; Jones et al., 2015). Infiltration of 1.25 nM of coronatine into leaves of LA2109 and Moneymaker (susceptible to T1) resulted in a similar amount of chlorosis (
Resistance of LA2109 is Effective Against Other P. syringae Pathovars
[0099] To determine if LA2109 is resistant to other strains of P. syringae, we chose a subset of diverse strains from a recent study (Cai et al., 2011) and tested them first on a Pto-expressing tomato cultivar (RG-PtoR) to determine whether they lacked avrPto and avrPtoB and were virulent on tomato.
Development of an F2 Population to Investigate the Genetic Basis of T1 Resistance in LA2109
[0100] To further explore the resistance in LA2109, we initiated a genetic approach by crossing LA2109 to RG-PtoS (female parent), a variety fully susceptible to T1. An F1 plant, which was verified based on its morphology and later by DNA markers, successfully set seeds for an F2 population. Newly-emerged branches from the F1 and LA2109 plants were excised, rooted and grown for 6 weeks, and seedlings were inoculated with T1. Leaves of F1 plants developed a few specks seven days after inoculation, whereas no disease symptoms were observed on LA2109 (data not shown). As expected, RG-PtoS developed severe symptoms of speck disease.
Identification of Quantitative Trait Loci on Chromosomes 2 and 8
[0101] A total of 423 F2 plants along with the parents (LA2109 and RG-PtoS) were inoculated with T1 and disease symptoms were scored seven days later using a system in which LA2109, which developed no or very few specks was scored as a 1 and RG-PtoS, with many dead leaves, was scored as a 3. The F2 plants were similarly scored and classified into three groups: resistant (scored as a 1), moderately resistant (2), and susceptible (3) (
[0102] Segregation distortion was detected on chromosome 1 between approximately 2.5 Mbp and 70.3 Mbp and chromosome 6 between 0 Mbp and 34.3 Mbp, where no RG-PtoS homozygotes were detected in the F2 plants. Both of these chromosomes are known to have loci involved in unilateral incompatibility, where pistils of self-incompatible species reject the pollen of self-compatible species (ui1.1, on chromosome 1 which encodes an S-locus F-box protein, maps to approximately 33.3 Mbp-49.4 Mbp, near the self-incompatibility locus (Li and Chetelat, 2015) and ui6.1, which encodes a Cullin protein, is located on chromosome 6 at 49.6 mb (Li and Chetelat, 2010).
High Resolution Mapping of qRph1
[0103] To further test whether the candidate qRph1 is associated with resistance to T1, we designed one cleaved amplified polymorphic sequence (CAPS) marker (ZB3), four co-dominant insertion/deletion (INDEL) markers (ZB19, ZB8, ZB14 and ZB5) and three markers (ZB11, ZB12 and ZB16) with a large deletion in LA2109 that need two pairs of primers to genotype both parents at the same location. We used these 8 markers to genotype 49 T1-resistant F2 plants (selected from the population of 423 F2 plants) (
[0104] To further delimit the region containing qRph1, we combined our analysis of the 49 plants above with an additional 36 T1-resistant plants derived from the 423 F2 plants (for a total of 85 plants;
The Region Encompassing qRph1 has Several Potential Immunity-Related Genes Including Some Encoding RLPs and RLKs
[0105] By inspection of the tomato reference genome sequence we found that 139 genes are annotated in the 1,060 kb region defined above (Table 5). It is possible that LA2109 encodes additional genes in this region, but a high-quality S. habrochaites genome sequence is not available and our reads of LA2109 were insufficient for generating a de novo targeted assembly of this region. Among the 139 genes are some in classes which have been implicated in plant immunity, including two CBL-interacting kinases (Cipk1 and Cipk16; Cipk6 plays a role in Pst immunity, (de la Torre et al., 2013)), RPW8.2 (conferring broad-spectrum powdery mildew resistance, (Xiao et al., 2001)) as well as WRKY and bZIP transcription factors (Tsuda and Somssich, 2015).
[0106] Perhaps of greatest interest is the presence in this region of numerous genes encoding receptor-like proteins (RLPs) and receptor-like protein kinases (RLKs) (
One RLK-Encoding Gene is Highly Expressed in LA2109 and Other T1-Resistant Accessions but not in Rio Grande
[0107] For the remaining one RLP gene and 14 RLK genes we developed primers and examined the abundance of their transcripts in leaves of LA2109 and a pair of Rio Grande lines (RG-PtoR and RG-PtoS) that are near-isogenic for the Pto region and which were expected to be identical in the 1,060 kb segment (
Solyc02g072470 Transcript Abundance is Increased in Leaves Upon Treatment with MAMPs
[0108] The expression of genes encoding PRRs and PTI-related proteins is often induced upon treatment with MAMPs (Rosli et al., 2013, Zipfel et al., 2006). We therefore used quantitative RT-PCR to analyze expression of Solyc02g072470 six hours after treatment of leaves with FlgII-28 (Cal et al., 2011, Clarke et al., 2013) or Csp22 (Felix and Boller, 2003). Transcript abundance of this gene was increased in response to FlgII-28 in both RG-PtoS (4-fold) and LA2109 (1.5-fold) although the transcript abundance in LA2109 was much higher in uninduced leaves compared to RG-PtoS (
Solyc02g072470 has 18 Predicted LRRs and is a Class XII LRR Receptor-Like Kinase
[0109] The Solyc02g072470 gene is annotated as having four exons, and its protein is predicted to have 18 leucine-rich repeats (LRRs) and an intracellular kinase domain (
DISCUSSION
[0110] We initially discovered that S. habrochaites accession LA2109 showed no signs of speck disease after being inoculated with Pst T1 and subsequently found that such resistance is common among S. habrochaites accessions collected in a localized region in southern Ecuador. This finding raised the possibility that these accessions have a common genetic basis for this phenotype. To investigate the underlying mechanism involved, we focused on LA2109 as representative of these accessions and found that it also expressed a Pto-like activity. This was not unexpected as another S. habrochaites accession and other wild relatives of tomato have been reported to have Pto (Riely and Martin, 2001, Rose et al., 2005).
[0111] We observed that LA2109 is susceptible to two other sequenced Pst strains (NY-T1 and DC3000avrPtoavrPtoB) but a comparison of factors involved in host interactions among these three strains did not reveal any obvious explanations for the differential responses. This prompted us to use a mapping-by-sequencing approach that ultimately defined QTLs on chromosomes 2 (qRph1) and 8 (qRph2) that are associated with T1 resistance. Recombination suppression on chromosome 8 impeded the ability to further delineate the boundaries of qRph2. The region containing qRph1 on chromosome 2 was further delimited by molecular markers and found to contain 139 genes. One gene provides a promising candidate for qRph1 as it is highly expressed in LA2109 and other T1-resistant S. habrochaites accessions, but not in RG-PtoS.
[0112] Our consideration of whether specific MAMPs might potentially explain the differential resistance of LA2109 to T1, DC3000avrPtoavrPtoB, and NY-T1 did not produce any likely candidates among the three known peptide MAMPs of Pst. It remains possible that another MAMP present in T1, but missing from the other two strains, is recognized by LA2109 and activates a strong PTI response. Consistent with this hypothesis, the essentially identical repertoire of type III effectors in T1 and NY-T1 suggests ETI is not involved in LA2109 resistance to T1. It is possible that amino acid differences in one or more effectors in T1 compared to NY-T1 allows it/them to be detected by LA2109. However, the lack of any R protein-like encoding genes in our candidate region also suggests ETI is not involved in the phenotype. Finally, we considered the possible involvement in LA2109 resistance of coronatine which is produced by NY-T1 and DC3000, but not by T1. However, we saw no difference in the sensitivity to this phytotoxin between LA2109 and another tomato cultivar susceptible to T1. A previous study also reported that production of coronatine does not correlate with Pst virulence (Kunkeaw et al., 2010). Collectively, the known differences among these three Pst strains did not suggest any factor that might explain the specific resistance in LA2109 to T1.
[0113] We discovered that although LA2109 is susceptible to NY-T1 and DC3000avrPtoavrPtoB, it is resistant to four diverse P. syringae pathovars (pathogens of celery and peach) which are able to cause disease on the Pto-expressing tomato line RG-PtoR. To date, we have identified just three strains that lack avrPto and avrPtoB and cause disease on LA2109-DC3000avrPtoavrPtoB, NY-T1 and Pst19 (a genome sequence is not available for Pst19).
[0114] Our initial analysis to understand the genetic basis of LA2109 resistance indicated that the phenotype was not segregating in a simple fashion and might be quantitative. By using a mapping-by-sequencing approach involving low coverage whole genome sequencing of F2 plants and higher coverage data from the parental accessions, we were able to identify two QTLs and, in the case of qRph1, identify markers to define candidate loci for conferring resistance. Strict filtering of F2 SNPs was necessary mainly due to divergence between the two parental accessions which resulted in uncertainty in the true mapping location of many reads derived from the LA2109 parent. While low-coverage data can not easily be used to accurately determine genotype at individual loci, by pooling loci and calling a consensus genotype for linked loci in the F2 population, we were able to make genotype calls.
[0115] Despite the complications due to low sequence coverage in the F2 plants, by using additional DNA marker-based mapping in a large F2 population, we were able to delimit a 1,060 kb region that is associated with resistance to T1. The genome sequence surrounding and including this region contains many genes encoding receptor-like kinase (RLKs), including the two FLS2 genes present in tomato (FLS2.1 and FLS2.2). We observed no difference in the transcript abundance of FLS2.1 in LA2109 and RG-PtoS and data from our QTL analysis and high-resolution mapping placed both genes outside of the region conferring T1 resistance. Within the 1,060 kb region 139 genes have been annotated in the most recent version of the tomato gene predictions (ITAG release SL2.4; (Fernandez-Pozo et al., 2015)). None of these genes encode NB-LRR proteins although there are several other genes which belong to classes that are implicated in defense responses.
[0116] We chose to investigate the genes encoding RLKs/RLPs in the 1,060 kb region because of the well-known role of these types of proteins in PTI. Our initial intention was to determine if some of these genes are not expressed in leaves so as to exclude them from being qRph1. However, of the 18 genes we examined, a transcript was detected for 15 (Solyc02g071800, Solyc02g07870 and Solyc02g072390 were not expressed in leaves). Interestingly, four of the RLK genes appear to be paralogs; they are clustered within a 57 kb region and likely arose by duplication events (Solyc02g072400, Solyc02g072440, Solyc02g072470, and Solyc02g072480). Two of these genes, Solyc02g072470 and Solyc02g072480, had higher transcript abundance in LA2109 than in RG-PtoS. Solyc02g072470 is especially striking in this regard and we found its transcript is also highly abundant in the other T1-resistant accessions that were collected near LA2109. Furthermore, we found that transcript abundance of Solyc02g072470 was increased in leaves exposed to FlgII-28 or Csp22. Such induced expression is a characteristic of genes which encode PRRs (Rosli et al., 2013). A recent study reported that LA2109 has a higher ROS response to Csp22 compared to RG-PtoS (Veluchamy et al., 2014). However, in an analysis of 139 RG-PtoS x LA2109 F2 plants we saw no correlation between response to Csp22 and resistance to T1 (R.sup.2=0.01274).
[0117] A screen of 278 accessions of tomato wild species for resistance to two race 1 Pst strains (A9 and 407) was reported recently (Thapa et al., 2015). Five accessions were resistant to these strains with two of them being from S. habrochaites (LA2869 and LA1777). Using a series of LA1777 introgression lines four QTLs were identified as contributing to A9 resistance, each of which explained 10-12% of the phenotypic variation. Of potential interest is one of these QTLs, bsRr1-2, located on chromosome 2 at position 95 cM of the genetic map for the LA1777 population. Although it is not possible to determine precisely how this position corresponds to the tomato reference genome coordinates, based on the positions of the markers used in the LA1777 map it appears that bsRr1-2 lies about 8,100 kb away from the 1,060 kb region we have identified. Nevertheless, it is possible that our locus and the ones described in that study contribute to a common pathway enhancing resistance to race 1 Pst strains. Regardless of whether they contribute to the same pathway or to different mechanisms, the introgression into tomato varieties of the qRph1 region and the four LA1777 QTLs has the potential to provide some level of resistance to the increasingly prevalent race 1 strains of Pst. In addition, in light of the fact that LA2109 is resistant to some diverse P. syringae pathovars, our data indicate that qRph1 provides protection to a broader range of bacterial pathogens as has been reported for EFR and Xa21 (Lacombe et al., 2010; Mendes et al., 2010; Tripathi et al., 2014).
TABLE-US-00003 TABLE 3 Illumina read mapping metrics. Data for parents and F2 individuals. R = resistant F2, MR =- medium resistant F2, S = susceptible F2 Fragments Mapped After Properly- in Sample Total Duplicate paired proper Coverage Name Fragments Removal Fragments pair (%) Depth (x) LA2109 212860803 207326783 130190120 61.2 PtoS 23601150 22331615 21509003 91.1 5.8 MR11 11470186 10986580 8354653 72.8 2.8 MR122 10616173 10289488 8404054 79.2 2.7 MR137 11692754 11324178 8302771 71.0 2.9 MR18 10976104 10353930 8111152 73.9 2.7 MR207 14785662 14286880 10907819 73.8 3.7 MR30 11976041 11606780 8834166 73.8 3.0 MR31 11734673 11224585 8824365 75.2 2.9 MR77 15483798 14823042 11671463 75.4 3.8 R100 10134809 9858146 6880893 67.9 2.5 R111 10759498 10605887 7594228 70.6 2.7 R112 10694082 10451856 8012251 74.9 2.7 R132 9826729 9584503 6755256 68.7 2.5 R149 10382194 9982260 7723191 74.4 2.6 R15 9276070 8874826 6941617 74.8 2.3 R155 12092987 11643784 8358687 69.1 3.0 R156 11328913 11048121 8058760 71.1 2.9 R17 11274233 10833868 7740262 68.7 2.8 R19 14271142 13868982 9952024 69.7 3.6 R198 12066307 11625942 8883321 73.6 3.0 R202 12539971 12103014 8426395 67.2 3.1 R215 10526919 10096309 7958825 75.6 2.6 R221 10301328 9993732 7370328 71.5 2.6 R222 10265270 9935362 7171611 69.9 2.6 R225 11566639 11291279 7745370 67.0 2.9 R47 13032836 12570891 9184205 70.5 3.2 R49 11216377 10707244 8143672 72.6 2.8 R59 10213359 9894728 7235657 70.8 2.6 R6 10968639 10460746 8171071 74.5 2.7 R64 12105419 11038845 8933151 73.8 2.9 R8 11370645 10871947 8363901 73.6 2.8 S1 10092947 9857648 7028058 69.6 2.5 S110 15378221 15000266 11785876 76.6 3.9 S116 12421933 12025389 9151256 73.7 3.1 S130 13726544 13217699 10092209 73.5 3.4 S131 13081800 12591614 9990173 76.4 3.3 S133 12974887 12509301 9294120 71.6 3.2 S135 12420298 12289756 9105888 73.3 3.2 S136 11709736 11545439 9286403 79.3 3.0 S139 11914648 11603624 8610644 72.3 3.0 S159 12072324 11733383 8443649 69.9 3.0 S16 8625015 8456381 6026048 69.9 2.2 S181 10349455 10061335 7583821 73.3 2.6 S183 14269559 13670370 10176089 71.3 3.5 S184 12874712 12318363 10360622 80.5 3.2 S193 13293565 12884042 9734696 73.2 3.3 S213 12218691 11760996 8548655 70.0 3.0 S26 9734480 9495351 7398605 76.0 2.5 S28 13758629 13200149 10569696 76.8 3.4 S36 16795027 16037982 11951111 71.2 4.1 S39 7911537 7709725 5712976 72.2 2.0 S43 13145628 12720574 9807838 74.6 3.3 S46 14678630 14313268.5 11394002 77.6 3.7 S55 13629556 13188313 9271526 68.0 3.4 S73 13016185 12602900.5 10130267 77.8 3.3 S83 13283243 12955998.5 10022596 75.5 3.3 S88 9990048 9756807.5 2.5 S89 11996453 11679334 9072422 75.6 3.0 S90 11501884 12591638 8587735 74.7 3.3 Average for F2 plants 73.0 3.0
TABLE-US-00004 TABLE4 Molecularmarkersusedforthehigh-resolutionmappingofRph1 Marker Primerlocation Primers RG-PtoS LA2109 ZB1 SL2.40ch02:32439779 CAGACCCAAATACAGTTGTAGATG 363bp 243bp SL2.40ch02:32440140 CTAGCAAATTATATCATGACATTCG ZB3 SL2.40ch02:34555022 GAATTGGCTTACTAAAGTGTTTGG DigestedbyHinfI, Notdigestedby SL2.40ch02:34555443 CAAGCAAAGCAACCTCATTCAACTCC 197bp&225bp HinfI,422bp ZB19 SL2.40ch02:34831393 AGCCAACTTTGGTTGAAGGTG 230bp 130bp SL2.40ch02:34831605 CTCCTTAGGGTAGAGATTCGCC ZB8 SL2.40ch02:35089735 AATGTGGCATCGCACGAAGCAC 383bp 156bp SL2.40ch02:35090118 CATTATCCATTGGGAATTTCTCC ZB14 SL2.40ch02:35363629 CTTTCTATTAATCTCTTCTCCCCCA 500bp 250bp SL2.40ch02:35364150 ACTATGGAATGGTTAGTGGAACCT ZB11-1 SL2.40ch02:35640449 GATTTGGTTGCCTGAAAGTTATTC x 250bp SL2.40ch02:35641543 CTCTTTGTATATAATTCTTTGAGAT ZB11-2 SL2.40ch02:35640674 GGCTCAATCCCGAAGTGGTT 512bp x SL2.40ch02:35641161 TCCAAGTCTTATTGGACAACTTTCT ZB12-1 SL2.40ch02:36160948 GTTTTTGGGTTTATTATGATCATTG x 250bp SL2.40ch02:36161444 GTGTCAAGAACACTGGTTGCA ZB12-2 SL2.40ch02:36160948 GTTTTTGGGTTTATTATGATCATTG 500bp x SL2.40ch02:36161948 CGTAGGTTATGGGTTGCTCGAGTC ZB16-1 SL2.40ch02:36413739 TTTTGAGTGCACTTGCTCCT x 234bp SL2.40ch02:36414522 GTCACTAGATGCTAAAGAAGGGC ZB16-2 SL2.40ch02:36413739 TTTTGAGTGCACTTGCTCCT 442bp x SL2.40ch02:36414160 ACTGCGTTAGTTGGGAGAGAG ZB5 SL2.40ch02:36700673 GTGAAGTCTCGAGAATATATTTGTC 554bp 361bp SL2.40ch02:36701226 GGCAAGAGGAACATGTTCCGTAAGG ZB21-1 SL2.40ch02:38827214 TGAGTTGTCGACGTCTATGATG 696bp 222bp SL2.40ch02:38827889 AGGATACTTGAGCAAAAGGCT ZB21-2 SL2.40ch02:38827214 TGAGTTGTCGACGTCTATGATG 378bp x SL2.40ch02:38827568 ACGTCCCAATTCCCATAAATTACT ZB22 SL2.40ch02:42479171 CCTTTTCAATTACCCTCGCTGG 450bp 222bp SL2.40ch02:42479799 AAGCTTTGTAACTCCAAGTATGTTT *x indicates no fragment amplified from this accession with these primers. For ZB3, digestion of the 422 bp PCR product with HinfI produces 197 bp & 225 bp fragments from RG-PtoS; the 422 bp product from LA2109 is not digested by this enzyme. Sequences are SEQ ID Nos: 43-72, from top to bottom.
TABLE-US-00005 TABLE6 Primersforthedeterminationoftranscript abundanceinFIG.7 Primerdesignation Nucleotidesequence FLS2.1 AATGGGAACTTGGACAACA(3)* Solyc02g070890-F: FLS2.1 ACACCAAAGCTGAATACATCTAC(4) Solyc02g070890-R: Solyc02g071790-F: ATGGTTTCAATCGATAAGTTGTAC(5) Solyc02g071790-R: CCGACTTCTTGAGGTATTCTCCCG(6) Solyc02g071800-F: ATGGGTCTTCAATGCATGTGTTG(7) Solyc02g071800-R: TAGGTCCAGTTCCTCTAGCTGAG(8) Solyc02g071810/ TACACCGTGACACTGCACTTTGC(9) Solyc02g071820-F: Solyc02g071810/ GGGCTTGAAATTAGCTTCAAAAG(10) Solyc02g071820-R: Solyc02g071860-F: ATGCCAAGAATCTTCAATTTC(11) Solyc02g071860-R: ATCCTGCCCTATGAGAGATAT(12) Solyc02g071870-F: ATGTTGCCACTAAAAAGAGCTG(13) Solyc02g071870-R: AGGAAGATTAATACCCTTAAGAG(14) Solyc02g071880-F: ATGTATCCAACCATAGCTTGG(15) Solyc02g071880-R: GGGAAGCTTTACCAATTCAGGTGGG (16) Solyc02g072070-F: TGGATCATTGTGCTCCTTGAGT(17) Solyc02g072070-R: CCAGCTCAAGGTCAGTCCAG(18) Solyc02g072250-F: TGGCTCCATTGTTCCTCTCA(19) Solyc02g072250-R: TGAACAGGAGAAACAGGGCA(20) Solyc02g072310-F: CATTGGGTGGCCACTACTCC(21) Solyc02g072310-R: TTCAGCAGGAATCGGACCAG(22) Solyc02g072390-F: ACAACTTGGCAACCTCTCCT(23) Solyc02g072390-R: GTTGTTGCCGAGTGCAAGATT(24) Solyc02g072400-F: GCAACTTGCTTAGCCATGAAT(25) Solyc02g072400-R: CGCAAACGAGAAAACTCAGGT(26) Solyc02g072430-F: CTTTGTTCAGACTGGGGAGAT(27) Solyc02g072430-R: TCACTTCTTTCCAAGACTCCGA(28)_ Solyc02g072440-F: ACCACGCAGCATATCCAACT(29) Solyc02g072440-R: GAGCTGGGTATAGATCCACCAA(30) Solyc02g072470-F: GGCAATCTGCCTCAAGAAATGG(31) Solyc02g072470-R: GGATGGAGGAACAGTACCAGT(32) Solyc02g072480-F: TGTCAAGCAACTGGACCTCA(33) Solyc02g072480-R: AGTGCTGGAGTTCTGGCAAA(34) Solyc02g072520-F: ACCTCCACTTGAATTCATCTACTCT (35) Solyc02g072520-R: CCATGAGACTATGCCAGTGAGG(36) Solyc02g076660-F: GGGGGCAGATGGAACACTTA(37) Solyc02g076660-R: AGCACCAAAAAGACGGTTTTCA(38) Solyc12g015870-F: CCATCCAAATGCTCCGATCGATGA (39) Solyc12g015870-R: TGCCTCTCAATGAAGCCTTGTTGC (40) Solyc02g072470qRT-F: TGATCAGTCCGCGCTTCTTT(41) Solyc02g072470qRT-R: GTGCCTAGAGCCACAAGTGA(42) *Numbers in parentheses are SEQ ID NOs.
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[0195] While certain of the preferred embodiments of the present invention have been described and specifically exemplified above, it is not intended that the invention be limited to such embodiments. Various modifications may be made thereto without departing from the scope and spirit of the present invention, as set forth in the following claims.