Tomato line CHI 12-94084

Abstract

The invention provides seed and plants of tomato line CHI 12-94084. The invention thus relates to the plants, seeds and tissue cultures of tomato line CHI 12-94084, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

Claims

1. A tomato plant comprising at least a first set of the chromosomes of tomato line CHI 12-94084, a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399.

2. A seed comprising at least a first set of the chromosomes of tomato line CHI 12-94084, a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399.

3. The plant of claim 1, which is inbred.

4. The plant of claim 1, which is hybrid.

5. The seed of claim 2, which is inbred.

6. The seed of claim 2, which is hybrid.

7. The seed of claim 2, wherein the seed produces an inbred plant of line CHI 12-94084.

8. A plant part of the plant of claim 1.

9. The plant part of claim 8, further defined as a leaf, an ovule, pollen, a fruit, or a cell.

10. A tissue culture of regenerable cells of the plant of claim 1, said cells comprising at least a first set of the chromosomes of tomato line CHI 12-94084, a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399.

11. The tissue culture according to claim 10, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and stalks.

12. A tomato plant regenerated from the tissue culture of claim 10, wherein said plant has all of the physiological and morphological characteristics of tomato line CHI 12-94084, a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399.

13. A method of vegetatively propagating the plant of claim 1 comprising the steps of: (a) collecting tissue capable of being propagated from the plant according to claim 1; (b) cultivating said tissue to obtain proliferated shoots; and (c) rooting said proliferated shoots to obtain rooted plantlets.

14. The method of claim 13, further comprising growing at least a first plant from said rooted plantlets.

15. A method of introducing a desired trait into a tomato line comprising: (a) crossing a plant of CHI 12-94084 with a second tomato plant that comprises a desired trait to produce F1 progeny, a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399; (b) selecting an F1 progeny that comprises the desired trait; (c) backcrossing the selected F1 progeny with a plant of line CHI 12-94084 to produce backcross progeny; (d) selecting backcross progeny comprising the desired trait; and (e) repeating steps (c) and (d) three or more times to produce selected fourth or higher backcross progeny that comprises the desired trait and otherwise comprises essentially all of the morphological and physiological characteristics of tomato line CHI 12-94084.

16. A tomato plant produced by the method of claim 15.

17. A method of producing a plant comprising an added trait, the method comprising introducing a transgene conferring the trait into a plant of line CHI 12-94084, a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399.

18. A plant produced by the method of claim 17.

19. The plant of claini 1, further comprising a transgene, wherein said plant otherwise comprises essentially all of morphological and characteristics of tomato line CHI 12-94084, a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399.

20. The plant of claim 19, wherein the transgene confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

21. A plant of tomato line CHI 12-94084 further comprising a single locus conversion, a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399, wherein said plant otherwise comprises essentially all of the morphological and physiological characteristics of tomato line CHI 12-94084.

22. The plant of claim 21, wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.

23. A method for producing a seed of a plant derived from line CHI 12-94084 comprising the steps of: (a) crossing a tomato plant of line CHI 12-94084 with itself or a second tomato plant; a sample of seed of said line having been deposited under ATCC Accession Number PTA-13399; and (b) allowing seed of a line CHI 12-94084-derived tomato plant to form.

24. The method of claim 23, further comprising the steps of: (c) selfing a plant grown from said CHI 12-94084-derived tomato seed to yield additional line CHI 12-94084-derived tomato seed; (d) growing said additional line CHI 12-94084-derived tomato seed of step (c) to yield additional line CHI 12-94084-derived tomato plants; and (e) repeating the selfing and growing steps of (c) and (d) to generate at least a first further line CHI 12-94084-derived tomato plant.

25. The method of claim 23, wherein the second tomato plant is of an inbred tomato line.

26. The method of claim 24, further comprising: (f) crossing the further CHI 12-94084-derived tomato plant with a different tomato plant to produce seed of a hybrid progeny plant.

27. A method of producing a tomato seed comprising crossing the plant of claim 1 with itself or a second tomato plant and allowing seed to form.

28. A method of producing a tomato fruit comprising: (a) obtaining the plant according to claim 1, wherein the plant has been cultivated to maturity; and (b) collecting a tomato from the plant.

Description

DETAILED DESCRIPTION OF THE INVENTION

(1) The invention provides methods and compositions relating to plants, seeds and derivatives of tomato hybrid 840701782, tomato line CHI 12-94083 and tomato line CHI 12-94084.

(2) Tomato hybrid 840701782 is an indeterminate variety with small fruited plum sized fruits. Hybrid 840701782 has a heavy set of uniform sized fruits and long clusters. Fruit color ranges from green to red in ripen stage. Fruits are firm and can be shipped over long distances. The variety is suited for growing under glasshouse (heated) conditions, in soil, as well as on rockwool, and can be grown under plastic in warmer areas.

(3) Tomato hybrid 840701782 has fruits of 12-18 grams, depending on the growing conditions, with good flavor, and high Bix levels. Hybrid 840701782 is resistant against ToMV (race 0,1,2), Verticlium dahliae, Fusarium oxysporum (races 1 and 2), and Fulvia fulvum (races A-E).

(4) A. Origin and Breeding History of Tomato Hybrid 840701782

(5) The parents of hybrid 840701782 are CHI 12-94083 and CHI 12-94084. These parents were created as follows:

(6) Both parental lines were developed in the breeding station in Naaldwijk (Netherlands).

(7) The Female parent, CHI 12-94083, also known as CHI-12-94083, was derived by selfing a hybrid released by ENZA Zaden in Year 1 for 8 generations between Year 1 and Year 4. In each generation the best plant was selected for best fruit type, best color, best fruit set, and best flavor. After 8 generations of inbreeding, the inbred line was consistent enough to be used as a parent for the 840701782 hybrid. The female line was tested during inbreeding and found to be resistant for Fulvia fulvum, Verticilium albo-atrum, and Fusarium oxysporum races 1,2.

(8) The Male parent, CHI 12-94084, also known as CHI-12-94084, was derived by selfing a BC2F1 hybrid for 6 generations during Year 1 and Year 4. The BCF1 hybrid was created in Year 1 by using an inbred line with good flavor derived from the hybrid Aromata (Rijk Zwaan) and the backcross parent 90787. The backcross parent 90787 was developed on the Canary Islands by Western Seeds International, combining local cherry inbreds with good flavor. The male parent carries resistance against Fulvia fulvum, Verticilium albo-atrum, and Fusarium oxysporum race 1,2 resistance, in addition to the resistance against ToMV races 0, 1, 2. The first F1 cross was made in Year 4.

(9) The parent lines are uniform and stable, as is a hybrid produced therefrom. A small percentage of variants can occur within commercially acceptable limits for almost any characteristic during the course of repeated multiplication. However no variants are expected.

(10) B. Physiological and Morphological Characteristics of Tomato Hybrid 840701782, Tomato Line Chi 12-94083 and Tomato Line Chi 12-94084

(11) In accordance with one aspect of the present invention, there is provided a plant having the physiological and morphological characteristics of tomato hybrid 840701782 and the parent lines thereof. A description of the physiological and morphological characteristics of such plants is presented in Tables 1-3.

(12) TABLE-US-00001 TABLE 1 Physiological and Morphological Characteristics of Hybrid 840701782 Comparison: Characteristic 840701782 Santalina 1. Seedling anthocyanin in hypocotyl of 2-15 cm present present seedling (Montfavet H 63.4) habit of 3-4 week old seedling normal normal 2. Mature Plant height 205.2 cm 182.9 cm growth type indeterminate indeterminate (Marmande VR, Saint -Pierre, San Marzano 2) form compact compact size of canopy (compared to others large large of similar type) habit sprawling sprawling (decumbent) 3. Stem anthocyanin coloration of upper absent or very weak medium third length of internode (between 1.sup.st and short (short short 4.sup.th inflorescence) (Dombito, Manific, Paso, Trend) branching sparse (Brehm's intermediate Solid Red, Fireball) branching at cotyledon or first leafy present present node number of nodes between first 1 to 4 1 to 4 inflorescence number of nodes between early (1.sup.st 1 to 4 1 to 4 to 2.sup.nd, 2.sup.nd to 3.sup.rd) inflorescences number of nodes between later 1 to 4 1 to 4 developing inflorescences pubescence on younger stems moderately hairy moderately hairy 4. Leaf type (mature leaf beneath the 3.sup.rd tomato tomato inflorescence) margins of major leaflets (mature deeply toothed or shallowly toothed or leaf beneath the 3.sup.rd inflorescence) cut, sps. toward base scalloped marginal rolling or wiltiness (mature absent absent leaf beneath the 3.sup.rd inflorescence) surface of major leaflets (mature leaf rugose (bumpy or rugose beneath the 3.sup.rd inflorescence) veiny) pubescence (mature leaf beneath the normal normal 3.sup.rd inflorescence) attitude (in middle third of plant) semi-drooping horizontal (Montfavet H 63.5) length medium (Lorena) medium width medium medium division of blade bipinnate (Lukullus, bipinnate Saint-Pierre) size of leaflets (in middle of leaf) medium (Marmande medium VR, Royesta) intensity of green color medium (Lucy) medium glossiness (as for 6) weak (Daniela) weak blistering (as for 6) weak (Daniela) medium size of blisters (as for 6) small (Husky medium Cherrie Red) attitude of petiole of leaflet in semi-erect (Blizzard, semi-erect relation to main axis (in middle of Marmande VR) leaf) 5. Inflorescence inflorescence type (2.sup.nd and 3.sup.rd truss) mainly uniparous mainly uniparous (Dynamo) type (3.sup.rd inflorescence) simple simple average number of flowers in 13.5 11.6 inflorescence (3.sup.rd inflorescence) leafy or running inflorescence (3.sup.rd absent absent inflorescence) 6. Flower calyx normal (lobes awl normal shaped) calyx-lobes approx. equaling approx. equaling corolla corolla corolla color yellow yellow style pubescence dense/present sparse (Saint-Pierre) anthers all fused into tube all fused into tube fasciation (1.sup.st flower of 2.sup.nd or 3.sup.rd occasionally present occasionally inflorescence) color yellow yellow (Marmande VR) 7. Fruit typical shape in longitudinal section elliptical elliptical (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) shape of transverse/cross section flattened flattened (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) shape of stem end (3.sup.rd fruit of 2.sup.nd or flat indented 3.sup.rd cluster) shape of blossom end (3.sup.rd fruit of 2.sup.nd flat to pointed/ flat to pointed or 3.sup.rd cluster) nippled (Cal J, Early Mech, Peto Gro) size of blossom scar very small (Cerise, very small Early Mech, Europeel, Heinz 1706, Peto Gro, Rio Grande) shape of pistil scar (3.sup.rd fruit of 2.sup.nd or dot dot 3.sup.rd cluster) peduncle: abscission layer (3.sup.rd fruit present (pedicellate) present of 2.sup.nd or 3.sup.rd cluster) (Montfavet H 63.5, Roma) length (from abscission layer to medium medium calyx) (only for varieties with (Montfavet H 63.4, abscission layers) Montfavet H 63.5) ribbing at peduncle end weak (Early Mech, absent or very weak Hypeel 244, Melody, Peto Gro, Rio Grande) depression at peduncle end weak (Futuria, absent or very weak Melody) size of stem/peduncle scar small (Early Mech, small Peto Gro, Rio Grande, Roma) point of detachment of fruit at at calyx attachment at calyx attachment harvest (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) length of dedicel (3.sup.rd fruit of 2.sup.nd or 16.4 mm 10.6 mm 3.sup.rd cluster) length of mature fruit (stem axis; 3.sup.rd 41.6 mm 42.5 mm fruit of 2.sup.nd or 3.sup.rd cluster) diameter of fruit at widest point (3.sup.rd 33.7 mm 35.8 mm fruit of 2.sup.nd or 3.sup.rd cluster) weight of mature fruit (3.sup.rd fruit of 18.4 grams 32.6 g 2.sup.nd or 3.sup.rd cluster) size medium medium (Alphamech, Diego) ratio length/diameter medium (Early medium Mech, Peto Gro) core present present size of core in cross section (in very small (Cerise) small relation to total diameter) number of locules only 2 (Early Mech, only 2 Europeel, San Marzano) surface slightly rough slightly rough base color (mature-green stage) light green (Lanai, light green VF 145-F5) pattern (mature-green stage) green-shouldered green-shouldered green shoulder (before maturity) present (Daniela, present Montfavet H 63.5) shoulder color if different from base dark green dark green extent of green shoulder (as for 34) medium (Erlidor, large Foxy, Montfavet H 63.5) intensity of green color of shoulder dark (Ayala, Erlidor, medium (as for 34) Xenon) intensity of green color of fruit (as light (Capello, light for 34) Duranto, Trust) color at maturity (full-ripe) red (Ferline, Daniela, red Montfavet H 63.5) color of flesh at maturity (full-ripe) red/crimson red crimson (Ferline, Saint- Pierre) flesh color uniform uniform locular gel color of table-ripe fruit yellow yellow firmness soft (Trend) soft shelf life very long (Ernesto) very long time of flowering early (Feria, early Primabel) time of maturity medium (Montfavet medium H 63.5) ripening (blossom-to-stem axis) blossom-to-stem end blossom-to-stem end ripening (peripheral to central radial outside in outside in axis) epidermis color yellow yellow epidermis normal easy-peel epidermis texture average average thickness of pericarp thin (Marmande VR) medium dry matter content (at maturity) high (Aloha, high Coudoulet) sensitivity to silvering insensitive insensitive (Marathon, Sano) 10. Chemistry and Composition of Full-Ripe Fruits pH 4.3 4.3 titratable acidity, as % citric 10.61 5.9 total solids (dry matter, seeds and 11.89 8.4 skin removed) soluble Solids as Brix 9.8 4.9 11. Phenology seeding to 50% flow (1 open on 46 days 46 days 50% of plants) seeding to once over harvest (if 96 days 103 days applicable) fruiting season long (Marglobe) long relative maturity in areas tested medium medium 12. Adaptation culture greenhouse greenhouse principle use(s) fresh market fresh market machine harvest not adapted not adapted regions to which adaptation has been Northeast, demonstrated northwest (Glasshouse environment) *These are typical values. Values may vary due to environment. Other values that are substantially equivalent are also within the scope of the invention.

(13) TABLE-US-00002 TABLE 2 Physiological and Morphological Characteristics of Line CHI-12-94083 Comparison: Characteristic CHI-12-94083 Santalina 1. Seedling anthocyanin in hypocotyl of 2-15 cm present absent seedling (Montfavet H 63.4) habit of 3-4 week old seedling normal normal 2. Mature Plant height 185 cm 187.5 cm growth type indeterminate indeterminate (Marmande VR, Saint -Pierre, San Marzano 2) form normal lax, open size of canopy (compared to others medium medium of similar type) habit semi-erect sprawling 3. Stem anthocyanin coloration of upper third weak (Montfavet absent or very weak H 63.5) length of internode (between 1.sup.st and short (short short 4.sup.th inflorescence) (Dombito, Manific, Paso, Trend) branching sparse (Brehm's Solid sparse Red, Fireball) branching at cotyledon or first leafy present present node number of nodes between first 1 to 4 1 to 4 inflorescence number of nodes between early (1.sup.st 1 to 4 1 to 4 to 2.sup.nd, 2.sup.nd to 3.sup.rd) inflorescences number of nodes between later 1 to 4 1 to 4 developing inflorescences pubescence on younger stems moderately hairy moderately hairy 4. Leaf type (mature leaf beneath the 3.sup.rd tomato tomato inflorescence) margins of major leaflets (mature deeply toothed or cut, shallowly toothed or leaf beneath the 3.sup.rd inflorescence) sps. toward base scalloped marginal rolling or wiltiness (mature absent slight leaf beneath the 3.sup.rd inflorescence) surface of major leaflets (mature leaf smooth smooth beneath the 3.sup.rd inflorescence) pubescence (mature leaf beneath the normal normal 3.sup.rd inflorescence) attitude (in middle third of plant) semi-erect (Allround semi-drooping Drakar, Vitador) length medium (Lorena) medium width medium medium division of blade bipinnate (Lukullus, bipinnate Saint-Pierre) size of leaflets (in middle of leaf) small (Tiny Tim) medium intensity of green color medium (Lucy) light glossiness (as for 6) weak (Daniela) weak blistering (as for 6) weak (Daniela) weak size of blisters (as for 6) small (Husky small Cherrie Red) attitude of petiole of leaflet in semi-erect (Blizzard, semi-erect relation to main axis (in middle of Marmande VR) leaf) 5. Inflorescence inflorescence type (2.sup.nd and 3.sup.rd truss) mainly uniparous mainly uniparous (Dynamo) type (3.sup.rd inflorescence) simple simple average number of flowers in 12 10 inflorescence (3.sup.rd inflorescence) leafy or running inflorescence (3.sup.rd occasional absent inflorescence) 6. Flower calyx normal (lobes awl normal shaped) calyx-lobes approx. equaling shorter than corolla corolla corolla color yellow yellow style pubescence dense/present sparse (Saint-Pierre) anthers all fused into tube all fused into tube fasciation (1.sup.st flower of 2.sup.nd or 3.sup.rd occasionally present frequently present inflorescence) color yellow yellow (Marmande VR) 7. Fruit typical shape in longitudinal section heart shaped elliptical (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) shape of transverse/cross section flattened angular (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) shape of stem end (3.sup.rd fruit of 2.sup.nd or indented flat 3.sup.rd cluster) shape of blossom end (3.sup.rd fruit of 2.sup.nd flat to pointed/ flat to pointed or 3.sup.rd cluster) nippled (Cal J, Early Mech, Peto Gro) size of blossom scar very small (Cerise, very small Early Mech, Europeel, Heinz 1706, Peto Gro, Rio Grande) shape of pistil scar (3.sup.rd fruit of 2.sup.nd or dot dot 3.sup.rd cluster) peduncle: abscission layer (3.sup.rd fruit present (pedicellate) present of 2.sup.nd or 3.sup.rd cluster) (Montfavet H 63.5, Roma) length (from abscission layer to medium short calyx) (only for varieties with (Montfavet H 63.4, abscission layers) Montfavet H 63.5) ribbing at peduncle end weak (Early Mech, absent or very weak Hypeel 244, Melody, Peto Gro, Rio Grande) depression at peduncle end weak (Futuria, weak Melody) size of stem/peduncle scar small (Early Mech, small Peto Gro, Rio Grande, Roma) point of detachment of fruit at at calyx attachment at calyx attachment harvest (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) length of dedicel (3.sup.rd fruit of 2.sup.nd or 14.3 mm 11.3 mm 3.sup.rd cluster) length of mature fruit (stem axis; 3.sup.rd 41.6 mm 35.1 mm fruit of 2.sup.nd or 3.sup.rd cluster) diameter of fruit at widest point (3.sup.rd 30.5 mm 28.9 mm fruit of 2.sup.nd or 3.sup.rd cluster) weight of mature fruit (3.sup.rd fruit of 2.sup.nd 16.2 grams 18.6 grams or 3.sup.rd cluster) size medium (Alphamech, medium Diego) ratio length/diameter large (Rimone, Rio medium Grande) core present present size of core in cross section (in very small (Cerise) very small relation to total diameter) number of locules only 2 (Early Mech, only 2 Europeel, San Marzano) surface slightly rough slightly rough base color (mature-green stage) yellow green light green pattern (mature-green stage) green- shouldered green-shouldered green shoulder (before maturity) present (Daniela, present Montfavet H 63.5) shoulder color if different from base dark green dark green extent of green shoulder (as for 34) medium (Erlidor, medium Foxy, Montfavet H 63.5) intensity of green color of shoulder dark (Ayala, Erlidor, medium (as for 34) Xenon) color at maturity (full-ripe) red (Ferline, Daniela, red Montfavet H 63.5) color of flesh at maturity (full-ripe) red/crimson red (Ferline, Saint-Pierre) flesh color with lighter and uniform darker areas in walls locular gel color of table-ripe fruit yellow yellow firmness soft (Trend) very soft shelf life very long (Ernesto) very long time of flowering early (Feria, medium Primabel) time of maturity medium (Montfavet medium H 63.5) ripening (blossom-to-stem axis) blossom-to-stem end blossom-to-stem end ripening (peripheral to central radial outside in inside out axis) epidermis color yellow colorless epidermis normal normal epidermis texture average average thickness of pericarp thin (Marmande VR) medium dry matter content (at maturity) high (Aloha, high Coudoulet) sensitivity to silvering insensitive insensitive (Marathon, Sano) 10. Chemistry and Composition of Full-Ripe Fruits pH 4.2 4.3 titratable acidity, as % citric 8.34 8.9 total solids (dry matter, seeds and 11.06 11.9 skin removed) soluble Solids as Brix 7.7 7.4 11. Phenology seeding to 50% flow (1 open on 50% 46 days 48 days of plants) seeding to once over harvest (if 91 days 96 days applicable) fruiting season long (Marglobe) long 12. Adaptation culture greenhouse greenhouse principle use(s) fresh market fresh market machine harvest not adapted not adapted regions to which adaptation has been Northeast, demonstrated northwest *These are typical values. Values may vary due to environment. Other values that are substantially equivalent are also within the scope of the invention.

(14) TABLE-US-00003 TABLE 3 Physiological and Morphological Characteristics of Line CHI-12-94084 Comparison: Characteristic CHI-12-94084 Santalina 1. Seedling anthocyanin in hypocotyl of 2-15 cm present absent seedling (Montfavet H 63.4) habit of 3-4 week old seedling normal normal 2. Mature Plant height 195 cm 187.5 cm growth type indeterminate indeterminate (Marmande VR, Saint -Pierre, San Marzano 2) form lax, open lax, open size of canopy (compared to others medium medium of similar type) habit sprawling sprawling (decumbent) 3. Stem anthocyanin coloration of upper third absent or very weak absent or very weak length of internode (between 1.sup.st and short (short short 4.sup.th inflorescence) (Dombito, Manific, Paso, Trend) branching sparse (Brehm's Solid sparse Red, Fireball) branching at cotyledon or first leafy present present node number of nodes between first 1 to 4 1 to 4 inflorescence number of nodes between early (1.sup.st 1 to 4 1 to 4 to 2.sup.nd, 2.sup.nd to 3.sup.rd) inflorescences number of nodes between later 1 to 4 1 to 4 developing inflorescences pubescence on younger stems sparsely hairy moderately hairy (scattered long hairs) 4. Leaf type (mature leaf beneath the 3.sup.rd tomato tomato inflorescence) margins of major leaflets (mature shallowly toothed or shallowly toothed or leaf beneath the 3.sup.rd inflorescence) scalloped scalloped marginal rolling or wiltiness (mature strong slight leaf beneath the 3.sup.rd inflorescence) onset of leaflet rolling early season early season surface of major leaflets (mature leaf rugose (bumpy or smooth beneath the 3.sup.rd inflorescence) veiny) pubescence (mature leaf beneath the normal normal 3.sup.rd inflorescence) attitude (in middle third of plant) semi-drooping semi-drooping (Montfavet H 63.5) length medium (Lorena) medium width medium medium division of blade bipinnate (Lukullus, bipinnate Saint-Pierre) size of leaflets (in middle of leaf) medium (Marmande medium VR, Royesta) intensity of green color medium (Lucy) light glossiness (as for 6) medium weak (Marmande VR) blistering (as for 6) weak (Daniela) weak size of blisters (as for 6) small (Husky small Cherrie Red) attitude of petiole of leaflet in semi-drooping semi-erect relation to main axis (in middle of (Montfavet H 63.5) leaf) 5. Inflorescence inflorescence type (2.sup.nd and 3.sup.rd truss) mainly uniparous mainly uniparous (Dynamo) type (3.sup.rd inflorescence) simple simple average number of flowers in 14.7 10 inflorescence (3.sup.rd inflorescence) leafy or running inflorescence (3.sup.rd absent absent inflorescence) 6. Flower calyx fleshy normal calyx-lobes approx. equaling shorter than corolla corolla corolla color yellow yellow style pubescence sparse sparse anthers all fused into tube all fused into tube fasciation (1.sup.st flower of 2.sup.nd or 3.sup.rd occasionally present frequently present inflorescence) color yellow yellow (Marmande VR) 7. Fruit typical shape in longitudinal section heart shaped elliptical (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) shape of transverse/cross section flattened angular (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) shape of stem end (3.sup.rd fruit of 2.sup.nd or indented flat 3.sup.rd cluster) shape of blossom end (3.sup.rd fruit of 2.sup.nd pointed/tapered flat to pointed or 3.sup.rd cluster) (Europeel, Heinz 1706, Hypeel 244, Roma VF) size of blossom scar very small (Cerise, very small Early Mech, Europeel, Heinz 1706, Peto Gro, Rio Grande) shape of pistil scar (3.sup.rd fruit of 2.sup.nd or dot dot 3.sup.rd cluster) peduncle: abscission layer (3.sup.rd fruit present (pedicellate) present of 2.sup.nd or 3.sup.rd cluster) (Montfavet H 63.5, Roma) length (from abscission layer to medium short calyx) (only for varieties with (Montfavet H 63.4, abscission layers) Montfavet H 63.5) ribbing at peduncle end weak (Early Mech, absent or very weak Hypeel 244, Melody, Peto Gro, Rio Grande) depression at peduncle end weak (Futuria, weak Melody) size of stem/peduncle scar small (Early Mech, small Peto Gro, Rio Grande, Roma) point of detachment of fruit at at calyx attachment at calyx attachment harvest (3.sup.rd fruit of 2.sup.nd or 3.sup.rd cluster) length of dedicel (3.sup.rd fruit of 2.sup.nd or 18.7 mm 11.3 mm 3.sup.rd cluster) length of mature fruit (stem axis; 3.sup.rd 42.5 mm 35.1 mm fruit of 2.sup.nd or 3.sup.rd cluster) diameter of fruit at widest point (3.sup.rd 36.1 mm 28.9 mm fruit of 2.sup.nd or 3.sup.rd cluster) weight of mature fruit (3.sup.rd fruit of 2.sup.nd 17.8 grams 18.6 grams or 3.sup.rd cluster) size medium (Alphamech, medium Diego) ratio length/diameter medium (Early Mech, medium Peto Gro) core present present size of core in cross section (in very small (Cerise) very small relation to total diameter) number of locules only 2 (Early Mech, only 2 Europeel, San Marzano) surface slightly rough slightly rough base color (mature-green stage) yellow green light green pattern (mature-green stage) green-shouldered green-shouldered green shoulder (before maturity) present (Daniela, present Montfavet H 63.5) shoulder color if different from base dark green dark green extent of green shoulder (as for 34) medium (Erlidor, medium Foxy, Montfavet H 63.5) intensity of green color of shoulder dark (Ayala, Erlidor, medium (as for 34) Xenon) color at maturity (full-ripe) red (Ferline, Daniela, red Montfavet H 63.5) color of flesh at maturity (full-ripe) red/crimson red (Ferline, Saint-Pierre) flesh color uniform uniform locular gel color of table-ripe fruit yellow yellow firmness soft (Trend) very soft shelf life very long (Ernesto) very long time of flowering late (Manific, Saint- medium Pierre) time of maturity very late (Daniela) medium ripening (blossom-to-stem axis) blossom-to-stem end blossom-to-stem end ripening outside in inside out epidermis color yellow colorless epidermis normal normal epidermis texture average average thickness of pericarp medium (Carmello, medium Europeel, Floradade, Heinz 1706, Montfavet H 63.5) dry matter content (at maturity) high (Aloha, high Coudoulet) sensitivity to silvering insensitive insensitive (Marathon, Sano) 10. Chemistry and Composition of Full-Ripe Fruits pH 4.2 4.3 titratable acidity, as % citric 8.38 8.9 total solids (dry matter, seeds and 8.56 11.9 skin removed) soluble Solids as Brix 6.8 7.4 11. Phenology seeding to 50% flow (1 open on 50% 57 days 48 days of plants) seeding to once over harvest (if 110 days 96 days applicable) fruiting season long (Marglobe) long 12. Adaptation culture greenhouse greenhouse principle use(s) fresh market fresh market machine harvest not adapted not adapted regions to which adaptation has been Northeast, demonstrated northwest *These are typical values. Values may vary due to environment. Other values that are substantially equivalent are also within the scope of the invention.

(15) C. Breeding Tomato Plants

(16) One aspect of the current invention concerns methods for producing seed of tomato hybrid 840701782 involving crossing tomato lines CHI 12-94083 and CHI 12-94084. Alternatively, in other embodiments of the invention, hybrid 840701782, line CHI 12-94083, or line CHI 12-94084 may be crossed with itself or with any second plant. Such methods can be used for propagation of hybrid 840701782 and/or the tomato lines CHI 12-94083 and CHI 12-94084, or can be used to produce plants that are derived from hybrid 840701782 and/or the tomato lines CHI 12-94083 and CHI 12-94084. Plants derived from hybrid 840701782 and/or the tomato lines CHI 12-94083 and CHI 12-94084 may be used, in certain embodiments, for the development of new tomato varieties.

(17) The development of new varieties using one or more starting varieties is well known in the art. In accordance with the invention, novel varieties may be created by crossing hybrid 840701782 followed by multiple generations of breeding according to such well known methods. New varieties may be created by crossing with any second plant. In selecting such a second plant to cross for the purpose of developing novel lines, it may be desired to choose those plants which either themselves exhibit one or more selected desirable characteristics or which exhibit the desired characteristic(s) when in hybrid combination. Once initial crosses have been made, inbreeding and selection take place to produce new varieties. For development of a uniform line, often five or more generations of selfing and selection are involved.

(18) Uniform lines of new varieties may also be developed by way of double-haploids. This technique allows the creation of true breeding lines without the need for multiple generations of selfing and selection. In this manner true breeding lines can be produced in as little as one generation. Haploid embryos may be produced from microspores, pollen, anther cultures, or ovary cultures. The haploid embryos may then be doubled autonomously, or by chemical treatments (e.g. colchicine treatment). Alternatively, haploid embryos may be grown into haploid plants and treated to induce chromosome doubling. In either case, fertile homozygous plants are obtained. In accordance with the invention, any of such techniques may be used in connection with a plant of the invention and progeny thereof to achieve a homozygous line.

(19) Backcrossing can also be used to improve an inbred plant. Backcrossing transfers a specific desirable trait from one inbred or non-inbred source to an inbred that lacks that trait. This can be accomplished, for example, by first crossing a superior inbred (A) (recurrent parent) to a donor inbred (non-recurrent parent), which carries the appropriate locus or loci for the trait in question. The progeny of this cross are then mated back to the superior recurrent parent (A) followed by selection in the resultant progeny for the desired trait to be transferred from the non-recurrent parent. After five or more backcross generations with selection for the desired trait, the progeny have the characteristic being transferred, but are like the superior parent for most or almost all other loci. The last backcross generation would be selfed to give pure breeding progeny for the trait being transferred.

(20) The plants of the present invention are particularly well suited for the development of new lines based on the elite nature of the genetic background of the plants. In selecting a second plant to cross with 840701782 and/or tomato lines CHI 12-94083 and CHI 12-94084 for the purpose of developing novel tomato lines, it will typically be preferred to choose those plants which either themselves exhibit one or more selected desirable characteristics or which exhibit the desired characteristic(s) when in hybrid combination. Examples of desirable traits may include, in specific embodiments, high seed yield, high seed germination, seedling vigor, high fruit yield, disease tolerance or resistance, and adaptability for soil and climate conditions. Consumer-driven traits, such as a fruit shape, color, texture, and taste are other examples of traits that may be incorporated into new lines of tomato plants developed by this invention.

(21) D. Performance Characteristics

(22) As described above, hybrid 840701782 exhibits desirable traits, as conferred by tomato lines CHI 12-94083 and CHI 12-94084. The performance characteristics of hybrid 840701782 and tomato lines CHI 12-94083 and CHI 12-94084 were the subject of an objective analysis of the performance traits relative to other varieties. The results of the analysis are presented in Table 4.

(23) TABLE-US-00004 TABLE 4 Performance data of Tomato hybrid 840701782 Fruit: Fruit: Fruit: Mature typical Fruit: green Fruit: color of Titratable plant: shape in shape of shoulder color at flesh at acidity, Soluble height longitudinal blossom (before maturity maturity Fruit: Time of as Total solids as Characteristic (cm) section end maturity) (full-ripe) (full-ripe) firmness maturity pH % citric solids Brix 840701782 205.2 Elliptical Flat to Present red red/crimson soft medium 4.3 10.61 11.89 9.8 pointed Flavorino Elliptical Flat to Present red red/crimson soft medium 4.3 5.9 8.4 4.9 pointed Santalina 187.5 Elliptical flat to Present red red very soft medium 4.3 8.9 11.9 7.4 pointed DRC 564 195.4 Circular flat Absent red red/crimson soft medium 4.5 6.0 11.5 7.7 Florino 176.5 Circular indented Absent red red/crimson soft medium 4.2 8.8 8.8 6.9

(24) E. Further Embodiments of the Invention

(25) In certain aspects of the invention, plants described herein are provided modified to include at least a first desired heritable trait. Such plants may, in one embodiment, be developed by a plant breeding technique called backcrossing, wherein essentially all of the morphological and physiological characteristics of a variety are recovered in addition to a genetic locus transferred into the plant via the backcrossing technique. The term single locus converted plant as used herein refers to those tomato plants which are developed by a plant breeding technique called backcrossing, wherein essentially all of the morphological and physiological characteristics of a variety are recovered in addition to the single locus transferred into the variety via the backcrossing technique. By essentially all of the morphological and physiological characteristics, it is meant that the characteristics of a plant are recovered that are otherwise present when compared in the same environment, other than an occasional variant trait that might arise during backcrossing or direct introduction of a transgene.

(26) Backcrossing methods can be used with the present invention to improve or introduce a characteristic into the present variety. The parental tomato plant which contributes the locus for the desired characteristic is termed the nonrecurrent or donor parent. This terminology refers to the fact that the nonrecurrent parent is used one time in the backcross protocol and therefore does not recur. The parental tomato plant to which the locus or loci from the nonrecurrent parent are transferred is known as the recurrent parent as it is used for several rounds in the backcrossing protocol.

(27) In a typical backcross protocol, the original variety of interest (recurrent parent) is crossed to a second variety (nonrecurrent parent) that carries the single locus of interest to be transferred. The resulting progeny from this cross are then crossed again to the recurrent parent and the process is repeated until a tomato plant is obtained wherein essentially all of the morphological and physiological characteristics of the recurrent parent are recovered in the converted plant, in addition to the single transferred locus from the nonrecurrent parent.

(28) The selection of a suitable recurrent parent is an important step for a successful backcrossing procedure. The goal of a backcross protocol is to alter or substitute a single trait or characteristic in the original variety. To accomplish this, a single locus of the recurrent variety is modified or substituted with the desired locus from the nonrecurrent parent, while retaining essentially all of the rest of the desired genetic, and therefore the desired physiological and morphological constitution of the original variety. The choice of the particular nonrecurrent parent will depend on the purpose of the backcross; one of the major purposes is to add some commercially desirable trait to the plant. The exact backcrossing protocol will depend on the characteristic or trait being altered and the genetic distance between the recurrent and nonrecurrent parents. Although backcrossing methods are simplified when the characteristic being transferred is a dominant allele, a recessive allele, or an additive allele (between recessive and dominant), may also be transferred. In this instance it may be necessary to introduce a test of the progeny to determine if the desired characteristic has been successfully transferred.

(29) In one embodiment, progeny tomato plants of a backcross in which a plant described herein is the recurrent parent comprise (i) the desired trait from the non-recurrent parent and (ii) all of the physiological and morphological characteristics of tomato the recurrent parent as determined at the 5% significance level when grown in the same environmental conditions.

(30) New varieties can also be developed from more than two parents. The technique, known as modified backcrossing, uses different recurrent parents during the backcrossing. Modified backcrossing may be used to replace the original recurrent parent with a variety having certain more desirable characteristics or multiple parents may be used to obtain different desirable characteristics from each.

(31) With the development of molecular markers associated with particular traits, it is possible to add additional traits into an established germ line, such as represented here, with the end result being substantially the same base germplasm with the addition of a new trait or traits. Molecular breeding, as described in Moose and Mumm, 2008 (Plant Physiology, 147: 969-977), for example, and elsewhere, provides a mechanism for integrating single or multiple traits or QTL into an elite line. This molecular breeding-facilitated movement of a trait or traits into an elite line may encompass incorporation of a particular genomic fragment associated with a particular trait of interest into the elite line by the mechanism of identification of the integrated genomic fragment with the use of flanking or associated marker assays. In the embodiment represented here, one, two, three or four genomic loci, for example, may be integrated into an elite line via this methodology. When this elite line containing the additional loci is further crossed with another parental elite line to produce hybrid offspring, it is possible to then incorporate at least eight separate additional loci into the hybrid. These additional loci may confer, for example, such traits as a disease resistance or a fruit quality trait. In one embodiment, each locus may confer a separate trait. In another embodiment, loci may need to be homozygous and exist in each parent line to confer a trait in the hybrid. In yet another embodiment, multiple loci may be combined to confer a single robust phenotype of a desired trait.

(32) Many single locus traits have been identified that are not regularly selected for in the development of a new inbred but that can be improved by backcrossing techniques. Single locus traits may or may not be transgenic; examples of these traits include, but are not limited to, herbicide resistance, resistance to bacterial, fungal, or viral disease, insect resistance, modified fatty acid or carbohydrate metabolism, and altered nutritional quality. These comprise genes generally inherited through the nucleus.

(33) Direct selection may be applied where the single locus acts as a dominant trait. For this selection process, the progeny of the initial cross are assayed for viral resistance and/or the presence of the corresponding gene prior to the backcrossing. Selection eliminates any plants that do not have the desired gene and resistance trait, and only those plants that have the trait are used in the subsequent backcross. This process is then repeated for all additional backcross generations.

(34) Selection of tomato plants for breeding is not necessarily dependent on the phenotype of a plant and instead can be based on genetic investigations. For example, one can utilize a suitable genetic marker which is closely genetically linked to a trait of interest. One of these markers can be used to identify the presence or absence of a trait in the offspring of a particular cross, and can be used in selection of progeny for continued breeding. This technique is commonly referred to as marker assisted selection. Any other type of genetic marker or other assay which is able to identify the relative presence or absence of a trait of interest in a plant can also be useful for breeding purposes. Procedures for marker assisted selection are well known in the art. Such methods will be of particular utility in the case of recessive traits and variable phenotypes, or where conventional assays may be more expensive, time consuming or otherwise disadvantageous. Types of genetic markers which could be used in accordance with the invention include, but are not necessarily limited to, Simple Sequence Length Polymorphisms (SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858, specifically incorporated herein by reference in its entirety), and Single Nucleotide Polymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

(35) F. Plants Derived by Genetic Engineering

(36) Many useful traits that can be introduced by backcrossing, as well as directly into a plant, are those which are introduced by genetic transformation techniques. Genetic transformation may therefore be used to insert a selected transgene into a plant of the invention or may, alternatively, be used for the preparation of transgenes which can be introduced by backcrossing. Methods for the transformation of plants that are well known to those of skill in the art and applicable to many crop species include, but are not limited to, electroporation, microprojectile bombardment, Agrobacterium-mediated transformation and direct DNA uptake by protoplasts.

(37) To effect transformation by electroporation, one may employ either friable tissues, such as a suspension culture of cells or embryogenic callus or alternatively one may transform immature embryos or other organized tissue directly. In this technique, one would partially degrade the cell walls of the chosen cells by exposing them to pectin-degrading enzymes (pectolyases) or mechanically wound tissues in a controlled manner.

(38) An efficient method for delivering transforming DNA segments to plant cells is microprojectile bombardment. In this method, particles are coated with nucleic acids and delivered into cells by a propelling force. Exemplary particles include those comprised of tungsten, platinum, and preferably, gold. For the bombardment, cells in suspension are concentrated on filters or solid culture medium. Alternatively, immature embryos or other target cells may be arranged on solid culture medium. The cells to be bombarded are positioned at an appropriate distance below the macroprojectile stopping plate.

(39) An illustrative embodiment of a method for delivering DNA into plant cells by acceleration is the Biolistics Particle Delivery System, which can be used to propel particles coated with DNA or cells through a screen, such as a stainless steel or Nytex screen, onto a surface covered with target cells. The screen disperses the particles so that they are not delivered to the recipient cells in large aggregates. Microprojectile bombardment techniques are widely applicable, and may be used to transform virtually any plant species.

(40) Agrobacterium-mediated transfer is another widely applicable system for introducing gene loci into plant cells. An advantage of the technique is that DNA can be introduced into whole plant tissues, thereby bypassing the need for regeneration of an intact plant from a protoplast. Modern Agrobacterium transformation vectors are capable of replication in E. coli as well as Agrobacterium, allowing for convenient manipulations (Klee et al., Bio-Technology, 3(7):637-642, 1985). Moreover, recent technological advances in vectors for Agrobacterium-mediated gene transfer have improved the arrangement of genes and restriction sites in the vectors to facilitate the construction of vectors capable of expressing various polypeptide coding genes. The vectors described have convenient multi-linker regions flanked by a promoter and a polyadenylation site for direct expression of inserted polypeptide coding genes. Additionally, Agrobacterium containing both armed and disarmed Ti genes can be used for transformation.

(41) In those plant strains where Agrobacterium-mediated transformation is efficient, it is the method of choice because of the facile and defined nature of the gene locus transfer. The use of Agrobacterium-mediated plant integrating vectors to introduce DNA into plant cells is well known in the art (Fraley et al., Bio/Technology, 3:629-635, 1985; U.S. Pat. No. 5,563,055).

(42) Transformation of plant protoplasts also can be achieved using methods based on calcium phosphate precipitation, polyethylene glycol treatment, electroporation, and combinations of these treatments (see, e.g., Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al., Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature, 312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986; Marcotte et al., Nature, 335:454, 1988). Transformation of plants and expression of foreign genetic elements is exemplified in Choi et al. (Plant Cell Rep., 13: 344-348, 1994), and Ellul et al. (Theor. Appl. Genet., 107:462-469, 2003).

(43) A number of promoters have utility for plant gene expression for any gene of interest including but not limited to selectable markers, scoreable markers, genes for pest tolerance, disease resistance, nutritional enhancements and any other gene of agronomic interest. Examples of constitutive promoters useful for plant gene expression include, but are not limited to, the cauliflower mosaic virus (CaMV) P-35S promoter, which confers constitutive, high-level expression in most plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985), including in monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591, 1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990); a tandemly duplicated version of the CaMV 35S promoter, the enhanced 35S promoter (P-e35S); 1 the nopaline synthase promoter (An et al., Plant Physiol., 88:547, 1988); the octopine synthase promoter (Fromm et al., Plant Cell, 1:977, 1989); and the figwort mosaic virus (P-FMV) promoter as described in U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter (P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem; the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform virus promoter; a commelina yellow mottle virus promoter; and other plant DNA virus promoters known to express in plant cells.

(44) A variety of plant gene promoters that are regulated in response to environmental, hormonal, chemical, and/or developmental signals can also be used for expression of an operably linked gene in plant cells, including promoters regulated by (1) heat (Callis et al., Plant Physiol., 88:965, 1988), (2) light (e.g., pea rbcS-3A promoter, Kuhlemeier et al., Plant Cell, 1:471, 1989; maize rbcS promoter, Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-binding protein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones, such as abscisic acid (Marcotte et al., Plant Cell, 1:969, 1989), (4) wounding (e.g., wunl, Siebertz et al., Plant Cell, 1:961, 1989); or (5) chemicals such as methyl jasmonate, salicylic acid, or Safener. It may also be advantageous to employ organ-specific promoters (e.g., Roshal et al., EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988; Bustos et al., Plant Cell, 1:839, 1989).

(45) Exemplary nucleic acids which may be introduced to plants of this invention include, for example, DNA sequences or genes from another species, or even genes or sequences which originate with or are present in the same species, but are incorporated into recipient cells by genetic engineering methods rather than classical reproduction or breeding techniques. However, the term exogenous is also intended to refer to genes that are not normally present in the cell being transformed, or perhaps simply not present in the form, structure, etc., as found in the transforming DNA segment or gene, or genes which are normally present and that one desires to express in a manner that differs from the natural expression pattern, e.g., to over-express. Thus, the term exogenous gene or DNA is intended to refer to any gene or DNA segment that is introduced into a recipient cell, regardless of whether a similar gene may already be present in such a cell. The type of DNA included in the exogenous DNA can include DNA which is already present in the plant cell, DNA from another plant, DNA from a different organism, or a DNA generated externally, such as a DNA sequence containing an antisense message of a gene, or a DNA sequence encoding a synthetic or modified version of a gene.

(46) Many hundreds if not thousands of different genes are known and could potentially be introduced into a tomato plant according to the invention. Non-limiting examples of particular genes and corresponding phenotypes one may choose to introduce into a tomato plant include one or more genes for insect tolerance, such as a Bacillus thuringiensis (B.t.) gene, pest tolerance such as genes for fungal disease control, herbicide tolerance such as genes conferring glyphosate tolerance, and genes for quality improvements such as yield, nutritional enhancements, environmental or stress tolerances, or any desirable changes in plant physiology, growth, development, morphology or plant product(s). For example, structural genes would include any gene that confers insect tolerance including but not limited to a Bacillus insect control protein gene as described in WO 99/31248, herein incorporated by reference in its entirety, U.S. Pat. No. 5,689,052, herein incorporated by reference in its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, herein incorporated by reference in their entirety. In another embodiment, the structural gene can confer tolerance to the herbicide glyphosate as conferred by genes including, but not limited to Agrobacterium strain CP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat. No. 5,633,435, herein incorporated by reference in its entirety, or glyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No. 5,463,175, herein incorporated by reference in its entirety.

(47) Alternatively, the DNA coding sequences can affect these phenotypes by encoding a non-translatable RNA molecule that causes the targeted inhibition of expression of an endogenous gene, for example via antisense- or cosuppression-mediated mechanisms (see, for example, Bird et al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be a catalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desired endogenous mRNA product (see for example, Gibson and Shillito, Mol. Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNA which expresses a phenotype or morphology change of interest is useful for the practice of the present invention.

(48) G. Definitions

(49) In the description and tables herein, a number of terms are used. In order to provide a clear and consistent understanding of the specification and claims, the following definitions are provided:

(50) Allele: Any of one or more alternative forms of a gene locus, all of which alleles relate to one trait or characteristic. In a diploid cell or organism, the two alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes.

(51) Backcrossing: A process in which a breeder repeatedly crosses hybrid progeny, for example a first generation hybrid (F.sub.1), back to one of the parents of the hybrid progeny. Backcrossing can be used to introduce one or more single locus conversions from one genetic background into another.

(52) Crossing: The mating of two parent plants.

(53) Cross-pollination: Fertilization by the union of two gametes from different plants.

(54) Diploid: A cell or organism having two sets of chromosomes.

(55) Emasculate: The removal of plant male sex organs or the inactivation of the organs with a cytoplasmic or nuclear genetic factor or a chemical agent conferring male sterility.

(56) Enzymes: Molecules which can act as catalysts in biological reactions.

(57) F.sub.1 Hybrid: The first generation progeny of the cross of two nonisogenic plants.

(58) Genotype: The genetic constitution of a cell or organism.

(59) Haploid: A cell or organism having one set of the two sets of chromosomes in a diploid.

(60) Linkage: A phenomenon wherein alleles on the same chromosome tend to segregate together more often than expected by chance if their transmission was independent.

(61) Marker: A readily detectable phenotype, preferably inherited in codominant fashion (both alleles at a locus in a diploid heterozygote are readily detectable), with no environmental variance component, i.e., heritability of 1.

(62) Phenotype: The detectable characteristics of a cell or organism, which characteristics are the manifestation of gene expression.

(63) Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer to genetic loci that control to some degree numerically representable traits that are usually continuously distributed.

(64) Resistance: As used herein, the terms resistance and tolerance are used interchangeably to describe plants that show no symptoms to a specified biotic pest, pathogen, abiotic influence or environmental condition. These terms are also used to describe plants showing some symptoms but that are still able to produce marketable product with an acceptable yield. Some plants that are referred to as resistant or tolerant are only so in the sense that they may still produce a crop, even though the plants are stunted and the yield is reduced.

(65) Regeneration: The development of a plant from tissue culture.

(66) Royal Horticultural Society (RHS) color chart value: The RHS color chart is a standardized reference which allows accurate identification of any color. A color's designation on the chart describes its hue, brightness and saturation. A color is precisely named by the RHS color chart by identifying the group name, sheet number and letter, e.g., Yellow-Orange Group 19A or Red Group 41B.

(67) Self-pollination: The transfer of pollen from the anther to the stigma of the same plant.

(68) Single Locus Converted (Conversion) Plant: Plants which are developed by a plant breeding technique called backcrossing, wherein essentially all of the morphological and physiological characteristics of a tomato variety are recovered in addition to the characteristics of the single locus transferred into the variety via the backcrossing technique and/or by genetic transformation.

(69) Substantially Equivalent: A characteristic that, when compared, does not show a statistically significant difference (e.g., p=0.05) from the mean.

(70) Tissue Culture: A composition comprising isolated cells of the same or a different type or a collection of such cells organized into parts of a plant.

(71) Transgene: A genetic locus comprising a sequence which has been introduced into the genome of a tomato plant by transformation.

(72) H. Deposit Information

(73) A deposit of tomato hybrid 840701782 and inbred parent lines CHI 12-94083 and CHI 12-94084, disclosed above and recited in the claims, has been made with the American Type Culture Collection (ATCC), 10801 University Blvd., Manassas, Va. 20110-2209. The date of deposits was Dec. 17, 2012. The accession numbers for those deposited seeds of tomato hybrid 840701782 and inbred parent lines CHI 12-94083 and CHI 12-94084 are ATCC Accession No. PTA-13400, ATCC Accession No. PTA-13398, and ATCC Accession No. PTA-13399, respectively. Upon issuance of a patent, all restrictions upon the deposits will be removed, and the deposits are intended to meet all of the requirements of 37 C.F.R. 1.801-1.809. The deposits will be maintained in the depository for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced if necessary during that period.

(74) Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity and understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the invention, as limited only by the scope of the appended claims.

(75) All references cited herein are hereby expressly incorporated herein by reference.