Prophylactic agent and/or therapeutic agent for stress urinary incontinence
09603845 ยท 2017-03-28
Assignee
Inventors
Cpc classification
A61P13/02
HUMAN NECESSITIES
International classification
Abstract
An object to be solved by the present invention is to provide a superior prophylactic agent and/or therapeutic agent for stress urinary incontinence. The present invention provides a prophylactic agent and/or a therapeutic agent for stress urinary incontinence comprising an effective amount of 4-piperidyl diphenylpropoxyacetate or a salt thereof, and a pharmaceutical carrier.
Claims
1. A method for treating stress urinary incontinence, comprising the step of administering to a mammal 4-piperidyl diphenyipropoxyacetate represented by Formula (1), ##STR00002## or a salt thereof, in an amount effective for the treatment of stress urinary incontinence, in combination with a pharmaceutical carrier.
Description
BRIEF DESCRIPTION OF DRAWINGS
(1)
DESCRIPTION OF EMBODIMENTS
(2) The 4-piperidyl diphenylpropoxyacetate or a salt thereof of the present invention is known as a metabolite that is found when propiverine is administered to rat or human. A typical production process for this is disclosed, for example, in Patent Document 1.
(3) The compound of the present invention obtained in the above manner is capable of forming a salt, in particular, a pharmaceutically acceptable salt, through a known method.
(4) The compound of the present invention or a salt thereof can be isolated and purified by using known separation and purification means, such as condensation, solvent extraction, filtration, recrystallization, and various kinds of chromatography.
(5) The ,-diphenyl--n-propoxy acetic acid 1-methyl-4-piperidyl ester disclosed in Patent Document 2 differs from the compound of the present invention in that a methyl group is attached to the nitrogen atom of piperidine, and its effect is for increasing the bladder capacity after operations on the bladder and prostate, for lowering intravesical pressure due to miction (miktion) in the case of hypertonic bladder and for reducing painful bladder tenesmus of varying genesis, as well as for the treatment of pollakiuria, nocturia and nocturnal enuresis. The Examples in Patent Document 1 disclose that ,-diphenyl--n-propoxy acetic acid-1-methyl-4-piperidyl ester is effective for an increase in bladder capacity and for reducing frequency of urination, and particularly effective for nocturia. It is also reported that propiverine (,-diphenyl--n-propoxy acetic acid-1-methyl-4-piperidyl ester) is useful for the treatment of stress urinary incontinence (for example, Acta Urol Jpn(1998)44, 65-69); however, as described above, the test for measuring intravesical pressure when urinary leakage (LPP) of a rat occurs clarified that the compound of the present invention has a significant alleviation effect for stress urinary incontinence, and this effect is superior to that of propiverine (Example 2).
(6) Examples of diseases treatable by administering the therapeutic agent comprising the compound of the present invention include stress urinary incontinence. Further, since many mixed urinary incontinence cases are a combination of stress urinary incontinence and urge urinary incontinence, the therapeutic agent may also be used for the treatment of mixed urinary incontinence.
(7) The present invention provides a pharmaceutical composition comprising an effective amount of the compound of the present invention represented by Formula (1), or a pharmaceutically acceptable salt thereof.
(8) Examples of the pharmaceutically acceptable salt of the compound of the present invention include acid addition salts with organic acids or inorganic acids, including acid addition salts with inorganic acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, nitric acid, or phosphoric acid, and acid addition salts with organic acids such as formic acid, acetic acid, propionic acid, oxalic acid, malonic acid, succinic acid, fumaric acid, maleic acid, lactic acid, malic acid, citric acid, tartaric acid, carbonic acid, picric acid, methanesulfonic acid, para-toluenesulfonic acid, or glutamic acid.
(9) When the compound represented by Formula (1) or a salt thereof of the present invention is used as a medication, a pharmaceutical carrier can be added, and a suitable dosage form according to prevention and treatment purposes can be made. Examples of the dosage form include oral preparations, injections, suppositories, ointments, and patches. Of these, oral preparations are preferable. Such dosage forms can be formed by using methods conventionally known to a person skilled in the art.
(10) As the pharmaceutical carrier, various organic or inorganic carrier materials commonly used as pharmaceutical raw materials are usable. These are used as excipients, lubricants, binders, or disintegrants in solid formulations, or as solvents, solubilizing agents, suspending agents, isotonizing agents, buffers, or soothing agents in liquid formulations, etc. If necessary, other pharmaceutical additives such as preservatives, antioxidants, colorants, sweeteners, and the like may also be used.
(11) Oral solid formulations are prepared as follows. An excipient, optionally together with a binder, disintegrant, lubricant, colorant, sweetening/flavoring agent, etc., is added into the compound of the present invention to produce tablets, (coated or uncoated), granules, powders, capsules, or the like using a standard method. The additives used may be those commonly used in the field. Examples of usable excipients include lactose, sucrose, sodium chloride, glucose, starch, calcium carbonate, kaolin, microcrystalline cellulose, and silicic acid. Examples of usable binders include water, ethanol, propanol, simple syrup, liquid glucose, liquid starch, liquid gelatin, carboxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl starch, methyl cellulose, ethyl cellulose, shellac, calcium phosphate, and polyvinylpyrrolidone. Examples of usable disintegrants include dry starch, sodium alginate, agar powder, sodium hydrogen carbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, and lactose. Examples of usable lubricants include purified talc, stearic acid salts, borax, and polyethylene glycol. Examples of usable colorants include titanium oxide and iron oxide. Examples of usable sweetening/flavoring agents include sucrose, wild orange peel, citric acid, and tartaric acid.
(12) Liquid oral formulations are prepared as follows. A sweetening/flavoring agent, buffer, stabilizer, etc., are added to the compound of the present invention to produce internal liquid medicines, syrups, elixirs, or the like using a standard method. In this case, sweetening/flavoring agents as described above are usable. Examples of usable buffers include sodium citrate, etc., and examples of usable stabilizers include tragacanth, gum arabic, and gelatin.
(13) Injections are prepared as follows. A pH adjuster, buffer, stabilizer, isotonizing agent, topical anesthetic, etc., are added to the compound of the present invention to produce subcutaneous injections, intramuscular injections, or intravenous injections using a standard method. Examples of usable pH adjusters and buffers in this case include sodium citrate, sodium acetate, and sodium phosphate. Examples of usable stabilizers include sodium pyrosulfite, EDTA, thioglycolic acid, and thiolactic acid. Examples of usable topical anesthetics include procaine hydrochloride, and lidocaine hydrochloride. Examples of usable isotonizing agents include sodium chloride and glucose.
(14) Suppositories are prepared by first adding a pharmaceutical carrier known in related fields, such as polyethylene glycol, lanolin, cacao butter, fatty acid triglyceride, or the like, to the compound of the present invention, optionally together with Tween () and like surfactants, etc., and than preparing suppositories by using a standard method.
(15) Ointments are prepared as follows. An ordinary base, stabilizer, wetting agent, preservative, etc., generally used for the compound of the present invention are added as required to the compound of the present invention, and mixed and formulated using a standard method. Examples of usable bases include liquid paraffin, white petrolatum, white beeswax, octyldodecyl alcohol, and paraffin. Examples of usable preservatives include methyl parahydroxybenzoate, ethyl parahydroxybenzoate, and propyl parahydroxybenzoate.
(16) Patches can be prepared by coating an ordinary support with the above ointment, cream, gel, paste, etc., using a standard method. Examples of usable supports include woven or nonwoven fabrics made from cotton, staple fibers, and chemical fibers; and films and foam sheets of soft vinyl chloride, polyethylene, polyurethane, etc.
(17) The amount of the compound of the present invention or a salt thereof to be contained in such a dosage unit form varies depending on the condition of the patient or on the dosage form. Generally, the desirable amount in one dosage unit form is about 0.01 to about 1000 mg in the case of an oral formulation, about 0.01 to about 500 mg in the case of an injection, and about 0.01 to about 1000 mg in the case of a suppository. The daily dose of the therapeutic agent in such a dosage form depends on the condition, body weight, age, gender, and the like of the patient. For example, the daily dose for an adult may be usually about 0.05 to about 5000 mg, and preferably 0.1 to 1000 mg, and is preferably administered at once or in two to four divided doses per day. In the present invention, the compound represented by Formula (1) or a salt thereof may be used solely or in a combination of two or more kinds.
(18) Examples of mammals to which the compound of the present invention is to be administered include humans, monkeys, mice, rats, rabbits, dogs, cats, cows, horses, pigs, and sheep.
(19) The present invention is more specifically explained below in reference to Examples and Test Examples. However, the present invention is not limited to these examples.
EXAMPLES
Example 1
Evaluation of Pharmaceutical Effect on a Stress Urinary Incontinence Model
(20) A group consisting of eight female rats intramuscularly administered botulinum toxin A was given a subcutaneous electrical stimulus in the abdominal region so as to increase abdominal pressure, thereby expressing urinary incontinence. The compound of the present invention (30 mg/kg) was orally administered to the model rats. Further, 2 mL/kg of distilled water was orally administered to the control group, and the normal group not treated with botulinum toxin A. The frequency of urinary incontinence of each group was counted before the administration, and 30 minutes and 2 hours after the administration. Table 1 shows the results.
(21) As shown in Table 1, although urinary incontinence was not observed in the normal group after the electrical stimulus subcutaneously delivered to the abdominal region, urinary incontinence was observed in all rats in the control group before the administration, and 30 minutes and 2 hours after the administration. In the group administered the compound of the present invention, even though urinary incontinence due to electrical stimulus was observed before the administration, the number of rats that express urinary incontinence due to the electrical stimulus was significantly decreased 2 hours after the administration of the compound of the present invention. The results showed that the compound of the present invention alleviated the symptoms of urinary incontinence of a stress urinary incontinence model.
(22) TABLE-US-00001 TABLE 1 Frequency of urinary incontinence 30 minutes 2 hours Before after after Number of admin- admin- admin- Therapeutic agent cases istration istration istration Normal group 8 0 0 0 Control group 8 8 8 8 Group administered 8 8 4 3* the compound of the present invention *p < 0.05; Significant difference from pre-data (Fisher's exact test)
Example 2
Leak Point Pressure (LPP) of a Rat
(23) The test for measuring intravesical pressure when urinary leakage (LPP) of a rat occurs is generally used as an in vivo test for evaluating a therapeutic effect for stress urinary incontinence (Am J Physiol Renal Physiol 293: F920-F926, 2007).
(24) A group consisting of six or seven female SD rats was intraperitoneally administered 1.2 g/kg of urethane and anesthetized. Then, each rat was fixed prone, and the spinal cord between the ninth thoracic vertebra and the tenth thoracic vertebra was completely cut off. After hemostasis, each rat was fixed supine, and the abdominal region was cut open by median incision. The bladder was exposed and the top of the bladder was cut open, and then an indwelling catheter for measuring intravesical pressure was placed. After the operation to place the indwelling catheter, abdominal pressure was forcibly applied manually from outside. The intravesical pressure when urinary leakage occurred was monitored and used as the LPP. Physiological saline, the compound of the present invention (3 mg/kg), and propiverine (3 mg/kg) as a control therapeutic agent were intravenously administered in an amount of 1 mL/kg. Five minutes after the administration, the LPP was measured and the average value and the standard error were calculated.
(25) The LPP was significantly higher in the group administered the compound of the present invention, compared with the physiological saline group. In contrast, there was no significant difference between the propiverine group and the physiological saline group. It was thus revealed that the compound of the present invention has an excellent therapeutic effect for stress urinary incontinence, and this effect is superior to that of propiverine.
INDUSTRIAL APPLICABILITY
(26) The compound represented by Formula (1) or a salt thereof of the present invention is useful as a prophylactic agent and/or a therapeutic agent for stress urinary incontinence.