Visual performance and/or macular pigmentation

Abstract

Disclosed is a composition comprising MZ for use as a dietary supplement or food additive for oral consumption for improving the visual performance of a human subject.

Claims

1. A capsule consisting essentially of isolated mesozeaxanthin, isolated lutein, isolated zeaxanthin, fish oil containing an omega-3 fatty acid and Vitamin E.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) The invention will now be further described by way of illustrative embodiment and with reference to the accompanying drawings, in which:

(2) FIG. 1 is a schematic representation of the structural formulae for lutein, zeaxanthin and MZ;

(3) FIG. 2 is a graph of corrected visual acuity against central macular pigment OD (arbitrary units) in a group of mixed normal and AMD subjects;

(4) FIG. 3 is a graph of macular pigment OD (at 0.25 eccentricity) against time for subjects consuming various macular carotenoid compositions;

(5) FIG. 4 is a graph showing the macular pigment OD measurement, at varying degrees of eccentricity, for particular subjects found to have atypical MPOD profiles, with a central dip (i.e. lower levels of macular pigment in the centre of the macula); meanSD MPOD values showing the central dip in the spatial profile of MP. Temporal MPOD values were measured at 0.25, 0.5, 1, 1.75, 3 of eccentricity. Specifically, given the known symmetry of MPOD, the MPOD values for negative eccentricities were assumed to be the same and constructed for the purpose of illustration;

(6) FIG. 5 is a graph of MPOD against time, for subjects receiving one of three different macular carotenoid formulations, wherein mean macular pigment optical density is measured at 0.25 retinal eccentricity at baseline, 4 weeks, and 8 weeks according to group wise; n=31; Group 1: high L group; Group 2: mixed carotenoid group; Group 3: high MZ group;

(7) FIG. 6 is a graph of MPOD against time, for subjects receiving one of three different macular carotenoid formulations, wherein mean macular pigment optical density is measured at 0.50 retinal eccentricity at baseline, 4 weeks, and 8 weeks according to group wise; n=31; Group 1: high L group; Group 2: mixed carotenoid group; Group 3: high MZ group;

(8) FIG. 7 is a graph of mean MPOD against retinal eccentricity for Group 1 respectively (see example 2), before and after an 8 week period of dietary supplementation, wherein the mean macular pigment optical density spatial profile of Group 1 is measured at baseline (pre supplementation) and at 8 weeks (post supplementation); mean+standard deviation; n=11; Group 1: high L group;

(9) FIG. 8 is a graph of mean MPOD against retinal eccentricity for Group 2 (see example 2), before and after an 8 week period of dietary supplementation, wherein the mean macular pigment optical density spatial profile of Group 2 is measured at baseline (pre supplementation) and at 8 weeks (post supplementation); mean+standard deviation; n=10; Group 2: combined carotenoid group;

(10) FIG. 9 is a graph of mean MPOD against retinal eccentricity for Group 3 (see example 2), before and after an 8 week period of dietary supplementation, wherein the mean macular pigment optical density spatial profile of Group 3 is measured at baseline (pre supplementation) and at 8 weeks (post supplementation); mean+standard deviation; n=10; Group 3: high MZ group;

(11) FIG. 10 is a graph of MPOD against retinal eccentricity (mean of eight subjects; see example 5) before (circular symbols) or after (square symbols) 3 months of supplementation with a daily dose of 10 mg L, 10 mg MZ and 10 mg Z.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

Example 1

Comparison of Macular Responses After Supplementation with Three Different Macular Carotenoid Formulations

(12) Subjects and Recruitment

(13) This study was conducted at the Institute of Vision Research, Whitfield Clinic, Waterford, Republic of Ireland. Seventy one subjects volunteered to participate in this study, which was approved by the local research ethics committee. Subjects were aged between 32 to 84 years and in good general health. The volunteers were divided into two groups: an AMD group and a normal group. 34 subjects had confirmed early stage AMD in at least one eye (AMD group; categorized and identified by either presence of drusen and/or pigmentary changes at the macula), and 37 subjects had no ocular pathology (normal group). Importantly, for the AMD group, significant efforts were made to identify patients with early AMD who were not currently taking carotenoid supplements.

(14) Study Design and Formulation

(15) L=Lutein MZ=mesozeaxanthin Z=Zeaxanthin

(16) This study was a single blind, randomized-controlled clinical trial of oral supplementation with three different macular carotenoid formulations, as follows:

(17) Group 1: high L group (n=24 [normal group=12 and AMD group=12]; L=20 mg/day, Z=2 mg/day);

(18) Group 2: mixed carotenoid group (n=24 [normal group=13 and AMD group=11]; MZ=10 mg/day, L=10 mg/day, Z=2 mg/day);

(19) Group 3: high MZ group (n=23 [normal group=12 and AMD group=11]; MZ=18 mg/day, L=2 mg/day L). All subjects were instructed to take one capsule (oil based) per day with a meal for 8 weeks. Compliance was assessed by tablet counting at each study visit.

(20) Measurement of Macular Pigment Optical Density (MPOD)

(21) The spatial profile of MP was measured using customized heterochromatic flicker photometry (cHFP) using the Macular Densitometer, a cHFP instrument that is slightly modified from a device described by Wooten & Hammond (2005 Optometry & Vision Science 82, 378-386) and by Kirby et al., (2010 Invest. Ophthalmol. Vis. Sci. 51, 6722-6728.

(22) Subjects were assessed at baseline, two weeks, four weeks, six weeks, and 8 weeks (V1, V2, V3, V4, and V5, respectively). MPOD was measured at the following eccentricities: at 0.25, 0.5, 1, 1.75, 3 but only results at 0.25, the central part of the retina corresponding to the macula, are reported here.

(23) Statistical Analysis The statistical software packages PASW Statistics 17.0 (SPSS Inc., Chicago, Ill., USA) and R were used for analysis and Sigma Plot 8.0 (Systat Software Inc., Chicago, Ill., USA) was used for graphical presentations. All quantitative variables investigated exhibited a typical normal distribution. We used the 5% level of significance.

(24) Results

(25) MPOD and Visual Acuity

(26) There was a positive and statistically significant relationship between central MPOD (at 0.25) and corrected visual acuity at baseline (r=0.303, p=0.008), as shown in FIG. 2 which is a graph of corrected visual acuity against MPOD (arbitrary units), showing the data points for individual subjects in the two groups prior to supplementation with one of the three carotenoid formulations. This finding suggests that central MP is significantly and positively related to visual performance.

(27) Increase in MPOD Over Time

(28) At an eccentricity of 0.25 the baseline MPOD was different for each group as follows; Group 1: 0.420.20 Group 2: 0.440.18 Group3: 0.490.21, with a mean of all groups of 0.450.20. To simplify the comparison all groups are drawn to start at the mean value. The study showed an increase in MPOD over time, as illustrated in FIG. 3, which is a graph of MPOD at 0.25 eccentricity (arbitrary units) against time (timepoints 1 to 5, corresponding to 0, 2, 4, 6 & 8 weeks respectively). As seen in FIG. 3, the biggest increase in central MPOD was achieved with the group 2 formulation (MZ=10 mg/day, L=10 mg/day, Z=2 mg/day) which was statistically significant different from groups 1 and 3. There was no statistical difference between group 1 and 3.

(29) Conclusions

(30) Surprisingly, the greatest effect on macular pigment was seen with the mixed carotenoid group (group 2) containing MZ10 mg L10 mg Z 2 mg, whereas results with the other two groups were very similar. There appears to be synergism between MZ & L. That the high MZ group (group 3) was able to increase MP demonstrates that MZ can raise MPOD substantially without any contribution from the other carotenoids, but was less effective than MZ in combination with L.

(31) Macular Carotenoid Supplementation in Subjects with Central Dips in Their Macular Pigment Spatial Profiles

(32) The central retina, known as the macula, is responsible for color and fine-detail vision (Hirsh & Curcio 1989; Vision Res. 29, 1095-1101). A pigment of the two dietary carotenoids, lutein (L) and zeaxanthin (Z), and a typically non-dietary carotenoid MZ (MZ), (Johnson et al., 2005 Invest. Ophtalmol. Vis. Sci. 46, 692-702) accumulates at the macula, where it is known as macular pigment (MP). MP is a blue light filter (Snodderly et al., 1984 Invest. Opthalmol. Vis. Sci. 25, 660-673) and a powerful antioxidant (Khachik et al. 1997 Invest. Ophthalm. & Vis. Sci. 38, 1802-1811), and is therefore believed to protect against age-related macular degeneration (AMD), which is now the most common cause of blind registration in the western world (Klaver et al., 1998 Arch. Ophthalmol. 116, 653-658).

(33) MZ and Z are the predominant carotenoids in the foveal region, whereas L predominates in the parafoveal region (Snodderley et al., 1991 Invest. Ophthalmol. Vis. Sci. 32, 268-279). The concentration of MZ peaks centrally, with an MZ:Z ratio of 0.82 in the central retina (within 3 mm of the fovea) and 0.25 in the peripheral retina (11-21 mm from the fovea) (Bone et al., 1997 Experimental Eye Research 64, 211-218). Retinal MZ is produced primarily by isomerization of retinal L, thus accounting for lower relative levels of L, and higher relative levels of MZ, in the central macula, and vice versa in the peripheral macula, and would also explain why MZ accounts for about one third of total MP.

(34) The concentration of MP varies greatly amongst individuals (Hammond et al., 1997 Journal of the Optical Society of America A-Optics Image Science & Vision, 14, 1187-1196). Atypical MP spatial profiles (i.e. central dips) are present in some individual MP profiles. More importantly, it was confirmed that these central dips were real and reproducible features of the MP spatial profile, when measured using customised heterochromatic flicker photometry (cHFP, a validated technique for measuring MP). The importance of such variations, if any, in the spatial profile of MP (e.g. the presence of a central dip) is not yet known, but may be related to the putative protective role of this pigment. For example, reduced MPOD at the centre of the macula (i.e. the presence of a central dip) may be associated with increased risk of developing AMD.

(35) It has been shown that 12% (58 subjects out of a sample database of 484 subjects) of the normal Irish population had a reproducible central dip in their MPOD spatial profile and that such a dip in the MP spatial profile is more common in older subjects and in cigarette smokers (two of the established risk factors for AMD).

Example 2

Supplementation of Formulations Containing Macular Carotenoids to Subjects with a Central Dip in Their MP Profile

(36) The study described in this example was performed with volunteer subjects from the above mentioned database (n=58) in the central dip study, who were identified, and confirmed, as having central dips in their MP spatial profile (i.e. MPOD at 0.5 degrees of eccentricity was MPOD at 0.25 degrees of eccentricity, see FIG. 4) and invited to participate in an 8-week supplementation trial with one of three different macular carotenoid formulations (see below).

(37) Methods

(38) Subjects and Study Design:

(39) Fifty eight subjects with central dips in their MP spatial profile (identified from a master MP database; n=484) were invited to take part in the study. Of the 40 subjects that agreed to come back for testing, 31 were confirmed as still having a central dip (i.e. MPOD at 0.5 degrees of eccentricity was MPOD at 0.25 degrees of eccentricity) and were therefore enrolled into the 8-week supplementation trial.

(40) All subjects signed an informed consent document and the experimental measures conformed to the Declaration of Helsinki. The study was reviewed and approved by the Research Ethics Committee, Waterford Institute of Technology, Waterford, Ireland. Inclusion criteria for participation in this study were as follows: MPOD at 0.5 degrees of eccentricity MPOD at 0.25 degrees of eccentricity (i.e. evidence of a central dip in the MP spatial profile); no presence of ocular pathology; visual acuity 20/60 or better in the study eye; not currently taking L and/or Z and/or MZ dietary supplements.

(41) Subjects were randomly assigned into one of the three groups as follows;

(42) Group 1: high L group (n=11), L=20 mg/day, Z=2 mg/day;

(43) Group 2: mixed carotenoid group (n=10), MZ=10 mg/day, L=10 mg/day, Z=2 mg/day.

(44) Group 3 the high MZ group (n=10), 18 mg/day MZ, 2 mg/day L).

(45) All subjects were instructed to take one capsule per day with a meal for 8 weeks. MPOD, including its spatial profile, i.e. at 0.25, 0.5, 1, 1.75, 3, was measured at baseline, four weeks and 8 weeks.

(46) Measurement of Macular Pigment Optical Density

(47) The spatial profile of MP was measured using cHFP using the Macular Densitometer, as described in Example 1. In order to measure the spatial profile of MP, measurements were made at the following degrees of retinal eccentricity: 0.25, 0.5, 1, 1.75, 3 and 7 (the reference point) obtained using the following sized target diameters; 30 minutes, 1, 2, 3.5, 1 and 2,

(48) Statistical Analysis

(49) The statistical software package PASW Statistics 17.0 (SPSS Inc., Chicago, Ill., USA) was used for analysis and Sigma Plot 8.0 (Systat Software Inc., Chicago, Ill., USA) was used for graphical presentations. All quantitative variables investigated exhibited a typical normal distribution. MeansSDs are presented in the text and tables. Statistical comparisons of the three different intervention groups, at baseline, were conducted using independent samples t-tests and chi-square analysis, as appropriate. We used the 5% level of significance throughout our analysis.

(50) Results

(51) Change in MPOD Over 8-Week Supplementation Period

(52) We conducted repeated measures ANOVA of MPOD, for all retinal eccentricities measured (i.e. at 0.25, 0.5, 1.0, 1.75, and 3), over time (i.e. over the study period [baseline, 4 weeks and 8 weeks]), using a general linear model approach, with one between-subjects factor: treatment (Group 1, Group 2, Group 3) and age as a covariate. FIGS. 5 and 6 show the change in MPOD during the course of the trial for measurements at 0.25 and 0.5 eccentricity respectively. Table 1 presents repeated measures ANOVA results for each group separately and for each degree of retinal eccentricity. As seen in this Table, increase in MPOD at 0.25 and 0.5 was statistically significant in Group 2 (i.e. the mixed carotenoids group). Similarly, a significant increase in MPOD at 0.25 was seen in Group 3 (i.e. high MZ group). Of note, only the increase in MPOD at 0.25 in Group 2 remains significant after Bonferroni correction for multiple testing.

(53) Change in the spatial profile of MPOD for each of Groups 1-3 is illustrated in FIGS. 7-9 respectively.

(54) Conclusions

(55) Only the two formulations containing MZ were able to correct the central dip and increase MP. Surprisingly, and contrary to expectation, the formulation containing L but without MZ had no effect on MPOD at any eccentricity.

(56) The formulation containing mixed carotenoids (group 2) had a superior effect since it increased MP significantly at both 0.25 and 0.5 eccentricities. This is consistent with the result from the subjects who received a supplement with all three carotenoids without a central dip at the baseline (see Example 1) i.e. the greatest response was observed using a supplement containing each of MZ, L and Z.

(57) TABLE-US-00001 TABLE 1 Average MPOD values at each degree of eccentricity for all subjects according to group & visit wise Time interaction Group MPOD Baseline 4 wks 8 wks (p-value) Group 1 0.25 0.45 0.20 0.48 0.22 0.49 0.21 0.112 Group 1 0.5 0.45 0.23 0.46 0.18 0.46 0.23 0.509 Group 1 1 0.20 0.18 0.27 0.15 0.25 0.13 0.234 Group 1 1.75 0.15 0.09 0.15 0.09 0.15 0.09 0.986 Group 1 3 0.15 0.11 0.16 0.09 0.11 0.08 0.265 Group 2 0.25 0.41 0.27 0.50 0.27 0.59 0.30 0.000 Group 2 0.5 0.44 0.26 0.46 0.28 0.52 0.28 0.016 Group 2 1 0.26 0.23 0.29 0.15 0.34 0.10 0.417 Group 2 1.75 0.18 0.10 0.19 0.06 0.22 0.06 0.218 Group 2 3 0.16 0.12 0.14 0.06 0.19 0.11 0.448 Group 3 0.25 0.48 0.16 0.55 0.19 0.57 0.18 0.005 Group 3 0.5 0.48 0.15 0.48 0.17 0.50 0.15 0.786 Group 3 1 0.32 0.12 0.31 0.13 0.34 0.12 0.596 Group 3 1.75 0.11 0.09 0.12 0.07 0.13 0.08 0.743 Group 3 3 0.12 0.08 0.15 0.07 0.15 0.07 0.522

(58) Values represent meanstandard deviation; n=31; MPOD=macular pigment optical density; 0.25=MPOD measured at 0.25 retinal eccentricity; 0.5=MPOD measured at 0.5 retinal eccentricity; 1.0=MPOD measured at 1.0 retinal eccentricity; 1.75=MPOD measured at 1.75 retinal eccentricity; 3=MPOD measured at 3.0 retinal eccentricity; Group 1: high L group; Group 2: combined carotenoid group; Group 3: high MZ group; the p-values represent repeated measures ANOVA for the 3 study visits (within-subject effects), with Greenhouse-Gesser correction for lack of sphericity as appropriate.

Example 3

Comparison of Visual Performance in Subjects with Early Stage AMD After Supplementation with Three Different Macular Carotenoid Formulations

(59) Subjects and Recruitment

(60) This study was conducted with 72 subjects, many with early AMD. For details see Example 1.

(61) Study Design and Formulation

(62) The subjects with were divided into 3 groups (of 20-27 subjects) and given the following supplementations:

(63) Group 1: L=20; Z=2 mg/day

(64) Group 2: L=10; MZ=10; Z=2 mg/day

(65) Group 3: MZ=17-18; L=2-3; Z=2 mg/day

(66) These formulations, dissolved in 0.3 ml vegetable oil, were administered in soft gel capsules.

(67) Visual Performance, using the techniques described previously above, was measured at baseline and at 3 and 6 months after supplementation. Statistical analyses were performed using a paired t test. Significant values were considered as P<0.05. Results are only given where at least one group was statistically significant.

(68) Results:

(69) Since there were no statistically significant improvements detected in VP after 3 months treatment, only results for 6 or 12 months are presented here (below):

(70) 1. Baseline Comparison Between Groups:

(71) TABLE-US-00002 TABLE 2 Baseline Comparison Group 2: Group 1: 10 mg MZ; Group 3: 20 mg L; 10 mg L; 18 mg MZ; Variable 2 mg Z 2 mg Z 2 mg L p N 23 27 22 Age 67 8 64 9 72 10 0.014 MPOD 0.25 0.412 0.19 0.482 0.21 0.475 0.20 0.411 BCVA 92 21 97 10 94 8 0.362

(72) The groups were statistically comparable at baseline with respect to MP and vision (assessed by Best Corrected Visual Acuity, BCVA). There was a significant difference between groups at baseline for age between Group 3 and the other two Groups. Group 1 and Group 2 were statistically similar with respect to age.

(73) 2. Best Corrected Visual Acuity (BCVA)

(74) There was a baseline correlation (before supplementation) of a positive and statistically significant relationship between central MPOD (0.25) and BCVA, importantly this is in the AMD population (r=0.368, p=0.002). There was no statistically significant change in BCVA in any group after 3 and 6 months.

(75) A computer-generated LofMAR test chart (Test Chart 2000 Pro; Thomson Software Solutions) was used to determine BCVA at a viewing distance of 4 m, using a Sloan ETDRS letterset. BCVA was determined as the average of three measurements, with letter and line changes facilitated by the software pseudo-randomization feature. Best corrected visual acuity was recorded using a letter-scoring visual acuity rating, with 20/20 (6/6) visual acuity assigned a value of 100. Best corrected visual acuity was scored relative to this value, with each letter correctly identified assigned a nominal value of one, so that, for example, a BCVA of 20/20+1 (6/6+1) equated to a score of 101, and 20/201 (6/61) to 99.

(76) 3. MPOD Response

(77) Table 3 below presents MP data for each Group and for each eccentricity measured, at baseline, six and twelve months after supplementation with macular carotenoids.

(78) A statistically significant increase in MPOD at 12 months was observed only in groups 2 and 3, receiving the MZ-containing supplement.

(79) TABLE-US-00003 TABLE 3 MPOD MPOD MPOD Group Baseline 6 months 12 months 0.25 0.25 p 1: 0.42 0.19 0.51 0.20 0.57 0.30 0.148 2: 0.48 0.22 0.58 0.21 0.63 0.19 0.001 3: 0.52 0.20 0.58 0.22 0.57 0.20 0.022 0.5 0.5 p 1: 0.32 0.19 0.42 0.18 0.46 0.27 0.126 2: 0.39 0.19 0.50 0.18 0.52 0.19 0.001 3: 0.41 0.19 0.46 0.19 0.45 0.20 0.034 1.0 1.0 p 1: 0.22 0.11 0.31 0.15 0.32 0.17 0.213 2: 0.25 0.12 0.36 0.17 0.37 0.18 0.001 3: 0.26 0.15 0.32 0.14 0.33 0.16 0.025 1.75 1.75 p 1: 0.13 0.10 0.18 0.11 0.20 0.10 0.114 2: 0.14 0.10 0.22 0.12 0.24 0.11 <0.001 3: 0.13 0.11 0.21 0.12 0.19 0.10 0.063

(80) 4. Letter Contrast Sensitivity (Thomson Chart)

(81) Table 4A presents letter contrast sensitivity data at baseline and six months after supplementation with macular carotenoids Measurements were made at 1.2, 2.4, 6.0, and 9.6 cpd. There was a statistically significant improvement only in Group 2 (10 mg MZ; 10 mg L; 2 mg Z) at 1.2, 2.4 and 9.6 cpd and not at all in the other two groups. This shows a greatly superior effect in Group 2.

(82) TABLE-US-00004 TABLE 4A Letter contrast Letter contrast sensitivity sensitivity Group Baseline Six months 1.2 cpd 1.2 cpd P 1: 1.68 0.34 1.75 0.30 0.091 2: 1.63 0.24 1.80 0.25 0.013 3: 1.68 0.37 1.63 0.25 0.322 2.4 cpd 2.4 cpd p 1: 1.60 0.33 1.66 0.34 0.17 2: 1.59 0.29 1.72 0.33 0.049 3: 1.61 0.35 1.63 0.32 0.6 9.6 cpd 9.6 cpd p 1: 1.1 0.36 1.04 0.41 0.194 2: 0.97 0.32 1.11 0.46 0.043 3: 0.94 0.37 0.95 0.43 0.901

(83) Table 4B shows the letter contrast sensitivity (CS) at baseline and 12 months, for each of five spatial frequencies (1.2-15.15 cpd).

(84) TABLE-US-00005 TABLE 4B Mean (sd) letter contrast sensitivity (CS) values at baseline and at 12 months. Group 1 Group 2 Group 3 cpd Baseline 12 months p Baseline 12 months p Baseline 12 months P 1.2 1.74 0.31 1.86 0.30 0.033 1.69 0.24 1.88 0.28 0.004 1.73 0.30 1.89 0.27 0.041 2.4 1.65 0.32 1.79 0.38 0.013 1.66 0.28 1.79 0.31 0.004 1.60 0.30 1.85 0.29 0.002 6.0 1.37 0.29 1.42 0.40 0.194 1.30 0.29 1.38 0.33 0.053 1.19 0.43 1.55 0.27 0.002 9.6 1.11 0.28 1.09 0.34 0.775 1.00 0.32 1.10 0.40 0.034 0.91 0.45 1.19 0.40 0.012 15.15 0.73 0.33 0.73 0.39 0.933 0.64 0.37 0.73 0.49 0.148 0.57 0.46 0.83 0.36 0.014 Abbreviations: cpd = cycles per degree

(85) At 12 months the results were similar to 6 months in that letter contrast sensitivity increased in all groups for large objects (1.2 and 2.4 cpd) but only in groups 2 and 3 with smaller objects (6.0-15.5 cpd).

(86) Table 4C reports the relationship between observed changes in MPOD (at 0.25 eccentricity) and observed changes in letter CS at 1.2 cpd. Of note, there were no statistically significant relationships between change in MP and change in letter CS, at any spatial frequency.

(87) TABLE-US-00006 TABLE 4C Change in MPOD vs. change in letter CS r p Group 1 0.262 0.294 Group 2 0.258 0.235 Group 3 0.043 0.875

(88) Colour Fundus Photographs

(89) Colour fundus photographs were taken at every study visit using a Zeiss VisuCam (Carl Zeiss Meditec A G, Jena, Germany) and were graded stereoscopically at the Ocular Epidemiology Reading Center at the University of Wisconsin, USA. Photographs were graded using a modified version of the Wisconsin Age-Related Maculopathy Grading System. Early AMD was defined as the presence of drusen and/or pigmentary changes in at least one eye, confirmed by an on-site ophthalmologist in collaboration with graders at the University of Wisconsin. Each fundus photograph was evaluated, lesion-by-lesion, to determine maximum drusen size, type, area, and retinal pigmentary abnormalities. Overall findings were reported on an 11-step AMD severity scale. A change of two or more steps along the severity scale was defined as being clinically significant. Graded photographs were obtained for baseline and 12 months visits.

(90) At baseline, there was no significant difference between the groups with respect to AMD grade (p=0.679) [Table 4D].

(91) TABLE-US-00007 TABLE 4D AMD grading for entire groups and subgroups at baseline. Entire group Group 1 Group 2 Group 3 Grade (n = 72) (n = 23) (n = 27) (n = 22) Sig. 1-3 16 (22.2%) 7 (30.4%) 6 (22.2%) 3 (13.6%) 0.679 4-5 28 (38.9%) 10 (43.5%) 8 (29.6%) 10 (45.5%) 6-7 19 (26.4%) 5 (21.7%) 8 (29.6%) 6 (27.3%) 8-9 4 (5.6%) 2 (7.4%) 2 (9.1%) 10-11 5 (6.9%) 1 (4.3%) 3 (11.1%) 1 (4.5%)

(92) The changes in AMD grade between baseline and 12 months for each of the three groups are summarized in Table 4E. A change in the negative direction (i.e. 1, 2) indicates a progression along the AMD severity scale, whereas positive integers indicate regression (improvement) along the AMD severity scale. Between baseline and 12 months, there was no statistically significant difference between treatment groups with respect to change in AMD severity (p=0.223, Pearson chi-square test).

(93) TABLE-US-00008 TABLE 4E Change in AMD grade (11-step scale) between baseline and 12 months. Group n 2 1 0 +1 +2 Sig. 1 16 1 (6%) 1 (6%) 10 3 (19%) 1 0.223 (63%) (6%) 2 23 1 (4%) 2 (9%) 14 4 (17%) 2 (61%) (9%) 3 15 2 (13%) 6 (40%) 4 2 (13%) 1 (27%) (7%) Total 54 4 (7%) 9 (17%) 28 9 (17%) 4 (100%) (52%) (7%) Abbreviations: n = number of subjects; negative value indicates disease progression; a positive value indicates disease regression; 0 = no change in grade

(94) Of note, table 4E shows that 86% of subjects exhibited no clinically significant change in the status of their AMD between baseline and 12 months, with 7% exhibiting deterioration and 7% exhibiting an improvement (note: a change in grade of two or more has been accepted as being clinically significant).

(95) Discussion

(96) The most interesting results were for letter contrast sensitivity. This test is only conducted in daylight and tests letters of different sizes. Results were at 6 months and 12 months were similar. There was no correlation between increase in MP and increase in this parameter indicating a neuro-physiological effects of macular carotenoids.

(97) There was no significant change in AMD grade from baseline. Thus changes in contrast sensitivity were not related to effects on AMD pathology.

(98) 5. Contrast Sensitivity at Night (Assessed on the FACT Device)

(99) Table 5 below presents log contrast sensitivity data assessed for night time, at baseline and six months after supplementation with macular carotenoids. Measurements were made at 1.5, 3.0, 6.0, 12 and 18 cpm. The statistically significant improvement in this measure of VP was present only in Group 2 at 1.5, 3.0, cpd and in Group 1 at 1.5 cpd showing a superior effect of group 2.

(100) TABLE-US-00009 TABLE 5 Night time contrast Night time contrast sensitivity sensitivity Group Baseline Six months 1.5 cpd 1.5 cpd P 1: 1.53 0.29 1.67 0.26 0.124 2: 1.51 0.27 1.66 0.3 0.028 3: 1.44 0.29 1.45 0.34 0.911 3.0 cpd 3.0 cpd p 1: 1.52 0.25 1.8 0.28 0.001 2: 1.62 0.34 1.75 0.41 0.01 3: 1.55 0.40 1.6 0.41 0.585

(101) 6. Contrast Sensitivity at Daytime (Assessed on the FACT Device)

(102) Table 6 below presents log contrast sensitivity data assessed for day time at baseline and six months after supplementation with macular carotenoids. Measurements were made at 1.5, 3.0. 6.0, 12 and 18 cpm. The statistically significant improvement in this measure of VP was present in Group 2 at 1.5, 3.0, and 18 cpd and in Group 1 at 1.5 cpd showing a superior effect in group 2.

(103) TABLE-US-00010 TABLE 6 Daytime contrast Daytime contrast sensitivity sensitivity Group Baseline Six months 1.5 cpd 1.5 cpd P 1: 1.41 0.16 1.57 0.26 0.03 2: 1.48 0.23 1.6 0.28 0.034 3: 1.41 0.13 1.5 0.28 0.238 3.0 cpd 3.0 cpd p 1: 1.67 0.21 1.75 0.21 0.17 2: 1.7 0.33 1.81 0.34 0.018 3: 1.72 0.18 1.77 0.29 0.46 18 cpd 18 cpd p 1: 0.62 0.4 0.56 0.41 0.497 2: 0.65 0.38 0.77 0.5 0.015 3: 0.57 0.4 0.62 0.43 0.704

(104) 7. Contrast Sensitivity at Night Time in the Presence of Glare (Assessed on the FACT Device)

(105) Table 7 below presents Log contrast sensitivity data at night in the presence of glare at baseline and six months after supplementation with macular carotenoids. Measurements were made at 1.5, 3.0, 6.0, 12 and 18 cpd. There was a statistically significant improvement in this VP only in Group 2 at 18 cpd.

(106) TABLE-US-00011 TABLE 7 Night time contrast Night time contrast sensitivity sensitivity with glare with glare Baseline Six months Group 18 cpd 18 cpd p 1: 0.34 0.16 0.34 0.16 0.136 2: 0.36 0.13 0.47 0.34 0.038 3: 0.36 0.22 0.32 0.08 0.588

(107) 8. Contrast Sensitivity at Day Time in the Presence of Glare (Assessed on the FACT Device)

(108) Table 8 below presents Log contrast sensitivity data at day time in the presence of glare, at baseline and six months after supplementation with macular carotenoids. Measurements were made at 1.5, 3.0, 6.0, 12 and 18 cpd. The statistically significant improvement in this measure of VP was present in Group 2 at 1.5, 3.0, 6.0, and 18 cpd cpd and in Group 1 at 1.5, 3.0, and 6.0 cpd and in Group 3 at 6 cpd, showing a superior effect in group 2.

(109) TABLE-US-00012 TABLE 8 Daytime contrast Daytime contrast sensitivity with sensitivity with glare glare Group Baseline Six months 1.5 cpd 1.5 cpd P 1: 1.5 0.25 1.63 0.21 0.001 2: 1.43 0.25 1.68 0.24 0.002 3: 1.42 0.38 1.46 0.36 0.351 3.0 cpd 3.0 cpd p 1: 1.68 0.22 1.85 0.22 0.006 2: 1.71 0.25 1.84 0.25 0.007 3: 1.67 0.35 1.71 0.43 0.542 6.0 cpd 6.0 cpd p 1: 1.46 0.42 1.85 0.22 <0.001 2: 1.46 0.47 1.84 0.25 <0.001 3: 1.36 0.42 1.71 0.43 0.001 18 cpd 18 cpd p 1: 0.64 0.46 0.59 0.43 0.642 2: 0.53 0.32 0.67 0.51 0.018 3: 0.7 0.47 0.66 0.45 0.609

(110) 9. Contrast Sensitivity and Glare Disability Between Baseline and 12 Months

(111) Data on contrast sensitivity (CS) and glare disability (GD) under mesopic (night-time) and photopic (daytime) conditions, at baseline and 12 months, are presented in Tables 9-12.

(112) TABLE-US-00013 TABLE 9 Log CS at baseline and 12 months under mesopic conditions (FACT device) Group p CS 1.5 cpd v1 CS 1.5 cpd v4 Group 1 1.59 0.28 1.80 0.22 0.007 Group 2 1.60 0.27 1.76 0.24 0.047 Group 3 1.53 0.39 1.73 0.25 0.124 CS 3 cpd v1 CS 3 cpd v4 Group 1 1.61 0.25 1.82 0.22 0.007 Group 2 1.68 0.34 1.80 0.26 0.058 Group 3 1.62 0.42 1.85 0.40 0.175 CS 6 cpd v1 CS 6 cpd v4 Group 1 1.18 0.38 1.24 0.53 0.521 Group 2 1.27 0.40 1.38 0.44 0.278 Group 3 1.20 0.44 1.46 0.50 0.060 CS 12 cpd v1 CS 12 cpd v4 Group 1 0.65 0.14 0.79 0.43 0.224 Group 2 0.67 0.26 0.79 0.24 0.080 Group 3 0.76 0.25 0.89 0.36 0.177 CS 18 cpd v1 CS 18 cpd v4 Group 1 0.40 0.25 0.32 0.08 0.207 Group 2 0.32 0.07 0.36 0.26 0.332 Group 3 0.36 0.15 0.39 0.24 0.476 Abbreviations: FACT = functional acuity contrast test; CS = contrast sensitivity; cpd = cycles per degree; v1 = baseline visit; v4 = 12 month visit

(113) TABLE-US-00014 TABLE 10 Log CS at baseline and 12 months under photopic conditions (FACT device) Group p CS 1.5 cpd v1 CS 1.5 cpd v4 Group 1 1.47 0.25 1.63 0.22 0.007 Group 2 1.56 0.21 1.61 0.24 0.478 Group 3 1.44 0.22 1.63 0.25 0.023 CS 3 cpd v1 CS 3 cpd v4 Group 1 1.70 0.22 1.86 0.11 0.002 Group 2 1.74 0.33 1.86 0.21 0.108 Group 3 1.78 0.20 1.84 0.24 0.402 CS 6 cpd v1 CS 6 cpd v4 Group 1 1.52 0.30 1.59 0.29 0.310 Group 2 1.52 0.39 1.66 0.39 0.064 Group 3 1.44 0.45 1.62 0.38 0.192 CS 12 cpd v1 CS 12 cpd v4 Group 1 1.01 0.33 0.98 0.35 0.709 Group 2 1.02 0.36 1.21 0.48 0.118 Group 3 0.99 0.43 1.19 0.48 0.164 CS 18 cpd v1 CS 18 cpd v4 Group 1 0.63 0.39 0.54 0.40 0.437 Group 2 0.59 0.38 0.64 0.48 0.687 Group 3 0.68 0.48 0.76 0.50 0.458 Abbreviations: FACT = functional acuity contrast test; GD = glare disability; cpd = cycles per degree; v1 = baseline visit; v4 = 12 month visit

(114) TABLE-US-00015 TABLE 11 Log GD at baseline and 12 months under mesopic conditions (FACT device) Group p GD 1.5 cpd v1 GD 1.5 cpd v4 Group 1 1.49 0.37 1.52 0.34 0.635 Group 2 1.44 0.39 1.53 0.35 0.365 Group 3 1.26 0.44 1.53 0.47 0.029 GD 3 cpd v1 GD 3 cpd v4 Group 1 1.57 0.43 1.60 0.32 0.728 Group 2 1.51 0.38 1.70 0.35 0.010 Group 3 1.39 0.50 1.55 0.49 0.346 GD 6 cpd v1 GD 6 cpd v4 Group 1 1.09 0.37 1.04 0.34 0.564 Group 2 1.18 0.35 1.24 0.43 0.581 Group 3 1.10 0.40 1.20 0.47 0.348 GD 12 cpd v1 GD 12 cpd v4 Group 1 0.66 0.17 0.71 0.18 0.343 Group 2 0.66 0.17 0.80 0.43 0.100 Group 3 0.77 0.24 0.69 0.22 0.115 GD 18 cpd v1 GD 18 cpd v4 Group 1 0.34 0.16 0.30 0.00 0.336 Group 2 0.34 0.10 0.39 0.37 0.483 Group 3 0.32 0.08 0.36 0.21 0.336 Abbreviations: FACT = functional acuity contrast test; GD = glare disability; cpd = cycles per degree; v1 = baseline visit; v4 = 12 month visit

(115) TABLE-US-00016 TABLE 12 Log GD at baseline and 12 months under photopic conditions (FACT device) Group p GD 1.5 cpd v1 GD 1.5 cpd v4 Group 1 1.60 0.25 1.76 0.23 0.006 Group 2 1.53 0.19 1.74 0.22 0.002 Group 3 1.51 0.25 1.69 0.42 0.058 GD 3 cpd v1 GD 3 cpd v4 Group 1 1.70 0.26 1.89 0.25 0.002 Group 2 1.78 0.21 1.97 0.18 0.001 Group 3 1.73 0.20 1.84 0.38 0.330 GD 6 cpd v1 GD 6 cpd v4 Group 1 1.54 0.38 1.64 0.35 0.358 Group 2 1.56 0.43 1.69 0.34 0.087 Group 3 1.46 0.47 1.71 0.38 0.048 GD 12 cpd v1 GD 12 cpd v4 Group 1 1.02 0.42 1.05 0.38 0.659 Group 2 0.97 0.36 1.14 0.35 0.169 Group 3 1.00 0.44 1.11 0.43 0.320 GD 18 cpd v1 GD 18 cpd v4 Group 1 0.64 0.45 0.67 0.48 0.752 Group 2 0.54 0.34 0.81 0.51 0.071 Group 3 0.75 0.48 0.75 0.52 0.993 Abbreviations: FACT = functional acuity contrast test; GD = glare disability; cpd = cycles per degree; v1 = baseline visit; v4 = 12 month visit

(116) Discussion

(117) Results at 12 months were similar to those at 6 months, in that the results were variable and difficult to interpret. Under mesopic (nighttime) conditions, contrast sensitivity only increased with large objects (1.5 and 3.0 cpd) in groups 1 and 2. For glare disability, group 1 did not change, whilst group 2 and 3 showed some change with large objects.

(118) Under photopic (daylight) conditions, groups 1 and 3 only increased contrast sensitivity with large objects. With glare disability all groups increased only with large objects.

(119) 10. Changes in Visual Performance Parameters and Changes in MPOD

(120) Table 13 reports the relationship between observed changes in MPOD (at 0.25 eccentricity) and observed changes in parameters of visual performance, namely CDVA and measures of mesopic and photopic contrast sensitivity, and mesopic and photopic glare disability, at 1.5 cpd. Of note, there were no statistically significant relationships between change in MP and change in visual performance in any of the groups (with the exception of a negative relationship between increases in MPOD and photopic CS at 1.5 cpd in Group 1 only).

(121) TABLE-US-00017 TABLE 13 r p Change in MPOD vs. change in CDVA Group 1 0.320 0.211 Group 2 0.148 0.558 Group 3 0.126 0.681 Change in MPOD vs. change in mesopic CS 1.5 cpd Group 1 0.055 0.859 Group 2 0.140 0.664 Group 3 0.041 0.906 Change in MPOD vs. change in photopic CS 1.5 cpd Group 1 0.705 0.007 Group 2 0.106 0.743 Group 3 0.122 0.720 Change in MPOD vs. change in mesopic GD 1.5 cpd Group 1 0.318 0.289 Group 2 0.106 0.743 Group 3 0.388 0.238 Change in MPOD vs. change in photopic GD 1.5 cpd Group 1 0.262 0.388 Group 2 0.136 0.673 Group 3 0.308 0.357 Abbreviations: MPOD = macular pigment optical density; CDVA = corrected distance visual acuity; L = lutein; Z = zeaxanthin; MZ = meso-zeaxanthin; CS = contrast sensitivity; cpd = cycles per degree; GD = glare disability.

(122) Discussion

(123) There was no correlation between increases in visual performance and increases in macular pigment, indicating a neuro-physiological effect of macular carotenoids.

(124) Other Conclusions: Changes in VP were Only Statistically Significant After 6 Months or More

(125) The methods reported here in contrast sensitivity were at varying spatial frequencies. Low spatial frequencies (e.g. 1.2 cpd) are indicative of very large objects (e.g. a car, a house), whereas, large spatial frequencies (e.g. 18 cpd) are indicative of small objects (e.g. a menu in a restaurant). The data lead to the following conclusions;

(126) 1. The most important effect was on contrast sensitivity which is one of the most important measures of VP and it reflects how the patient actually perceives their own vision.

(127) 2. Statistical significance was reached across many spatial frequencies, which means the improvement detected has implications for general and real life vision.

(128) 3. There was a superior improvement in VP for the Group 2 intervention (i.e. 10 mg MZ; 10 mg L; 2 mg Z).

Example 4

Effect of Two Macular Carotenoids and a Placebo Formulations on VP in Normal Subjects

(129) Subjects and Recruitment

(130) This study was conducted on 36 normal subjects with no AMD. Details of the recruitment are given in Example 1. Of the 36 subjects recruited, 32 completed the trial, with one drop-out from each of the intervention groups and two drop-outs from group 3, the placebo group. All further analysis was confined to those subjects with a complete data set (Group 1, n=11; Group 2, n=11; Group 3, n=10).

(131) Study Design and Formulations

(132) The normal subjects were divided into 3 groups of (initially) 12 subjects and given the following supplements:

(133) Group 1: L20; Z 2 mg/day

(134) Group 2: MZ 10; L 10; Z 2 mg/day

(135) Group 3: Placebo 0 mg/day

(136) The carotenoid formulations were in 0.3 ml vegetable oil and were administered in soft gel capsules.

(137) Visual performance was assessed as described in detail below, at baseline, 3 months and at 6 months.

(138) Statistical Analysis

(139) The statistical software package PASW Statistics 18.0 (SPSS Inc., Chicago, Ill., USA) was used for analysis. All quantitative variables investigated exhibited a typical normal distribution. MeansSDs are presented in the text and tables. Statistical comparisons of the three supplementation groups, at baseline, were conducted using one way ANOVA, while paired samples t tests and repeated measures ANOVA (using a general linear model approach) were used to analyze visual performance and MPOD measures in each supplementation group for change across study visits as appropriate. Where relevant, the Greenhouse-Geisser correction for violation of sphericity was used. A 5% level of significance was used throughout the analysis.

(140) Results

(141) 1. Baseline Analysis

(142) Following randomization, one-way analysis of variance revealed no significant differences between groups at baseline, in terms of demographic, macular pigment, visual performance parameters, or other parameters, as illustrated for selected parameters in table 14 below.

(143) TABLE-US-00018 TABLE 14 Group 1: Group 2: Group 3: Variable Mean SD Mean SD Placebo P value N 12 12 12 Age 56 8 51 13 46 20 0.3 BMI 27 3 25 3 26 5 0.31 BCVA 107 5 109 6 108 6 0.72 MPOD 0.25 0.32 0.13 0.37 0.13 0.35 0.18 0.69 MPOD 0.5 0.25 0.14 0.27 0.12 0.28 0.16 0.88 MPOD 1.0 0.15 0.14 0.20 0.07 0.16 0.11 0.46 MPOD 1.75 0.07 0.10 0.10 0.07 0.04 0.04 0.16 MPOD 3 0.07 0.08 0.08 0.07 0.04 0.05 0.26 SD = standard deviation; BMI = body mass index; BCVA = best corrected visual acuity; MPOD = macular pigment optical density

(144) 2. MPOD Response at 3 and 6 Months

(145) MPOD Measurement

(146) A spatial profile of MPOD was generated across 0.25, 0.5, 1, 1.75 and 3 of retinal eccentricity in relation to a 7 reference location, using the Macular Densitometer, which employs a heterochromatic flicker photometry (HFP) technique. Subjects were shown an explanatory video of the technique, and afforded a practice session prior to test commencement. HFP flicker frequencies were optimized following determination of individual critical flicker fusion (CFF) frequency measurements, in a customization process that optimizes MP measurements, (Stringham et al, Exp. Eye res. 2008, 87, 445-453). The MPOD measurement comprised the average of six readings (computed as the radiance value at which the subject reported null flicker) at each retinal eccentricity, and was deemed reliable and acceptable only when the standard deviation of null flicker responses was below 0.1

(147) TABLE-US-00019 TABLE 15 MPOD response and significance at each retinal eccentricity across study visits T RM Group Intervention Baseline 3 months T test 6 months Test ANOVA MPOD0.25 MPOD0.25 p* MPOD0.25 p** p*** 20 mg L; 2 mg Z 0.32 0.12 0.38 0.15 0.080 0.41 0.14 0.444 0.092 10 mg MZ; 10 mg L; 2 mg Z 0.37 0.13 0.49 0.14 0.002 0.50 0.20 0.012 0.002 Placebo 0.35 0.20 0.38 0.20 0.709 0.37 0.18 0.637 0.814 MPOD0.50 MPOD0.50 p MPOD0.50 P p 20 mg L; 2 mg Z 0.27 0.13 0.32 0.22 0.456 0.30 0.14 0.459 0.096 10 mg MZ; 10 mg L; 2 mg Z 0.28 0.12 0.38 0.16 0.011 0.37 0.21 0.042 0.010 Placebo 0.28 0.17 0.31 0.16 0.404 0.28 0.16 0.966 0.572 MPOD1.0 MPOD1.0 p MPOD1.0 P p 20 mg L; 2 mg Z 0.16 0.14 0.18 0.12 0.455 0.15 0.14 0.767 0.533 10 mg MZ; 10 mg L; 2 mg Z 0.21 0.08 0.28 0.10 0.035 0.27 0.14 0.085 0.047 Placebo 0.16 0.12 0.14 0.11 0.954 0.13 0.10 0.400 0.997 MPOD1.75 MPOD1.75 p MPOD1.75 P p 20 mg L; 2 mg Z 0.08 0.10 0.08 0.10 0.859 0.07 0.10 0.867 0.929 10 mg MZ; 10 mg L; 2 mg Z 0.11 0.07 0.19 0.05 0.005 0.18 0.10 0.041 0.036 Placebo 0.03 0.03 0.03 0.05 0.767 0.03 0.05 0.732 0.815 MPOD3.0 MPOD3.0 p MPOD3.0 P p 20 mg L; 2 mg Z 0.05 0.02 0.07 0.06 0.588 0.03 0.03 0.185 0.671 10 mg MZ; 10 mg L; 2 mg Z 0.09 0.07 0.11 0.11 0.275 0.10 0.07 0.707 0.915 Placebo 0.02 0.03 0.02 0.03 0.810 0.02 0.05 0.682 0.480 *difference between baseline and 3 months (paired samples t test) **difference between baseline and 6 months (paired samples t test) ***repeated measures ANOVA across all visits

(148) It can be seen here that the greatest increase in MP, at all eccentricities measured, can be seen in Group 2, a supplement containing 10 mg MZ; 10 mg L; 2 mg Z.

(149) Visual Performance Assessment

(150) Visual acuity (VA) was measured at baseline with a computer-generated logMAR test chart (Test Chart 2000 Pro; Thompson Software Solutions, Hatfield, UK) at a viewing distance of 4 m, using the Sloan ETDRS letterset. VA was measured using a single letter scoring visual acuity rating, and recorded as the average of three measurements facilitated by the software letter randomization feature. The eye with better visual acuity was chosen as the study eye; however, when both eyes had the same corrected acuity, the right eye was chosen as the study eye.

(151) Contrast sensitivity was measured using a functional acuity contrast test (Optec6500 Vision Tester; Stereo Optical Co. Inc, Chicago, Ill.), which incorporates sine wave gratings, presented as Gabor patches, at spatial frequencies of 1.5, 3, 6, 12 and 18 cycles per degree (cpd) to produce a contrast sensitivity function. Testing was performed under mesopic (3 candelas per square meter [cd/m.sup.2]) and photopic (85 cd/m2) conditions. (By way of explanation, 3 candelas per square meter is considered to represent the upper limit of mesopic conditions: any greater level of illumination is considered to constitute photopic conditions). Contrast sensitivity testing was performed using a Thomson Chart or using the EDTRS (Early Treatment Diabetic Retinopathy Study) letters in log MAR form at five different spatial frequencies (see Lorente-Velazquez et al., 2011 Optom. Vis Sci. 88 (10): 1245-1251).

(152) Glare disability was assessed using the same test, and testing conditions, but in the presence of an inbuilt circumferential LED glare source (42 lux for mesopic and 84 lux for photopic glare testing). The LED glare source rendered a daylight simulating color temperature of 6500 K, and a spectral emission profile with a single large peak at 453 nm (close to peak MP spectral absorbance). These tests have been described in more detail elsewhere (Loughman et al. Vis Res. 2010; 50:1249-1256; Nolan et al. Vis Res. 2011; 51:459-69). The subject task, and nature of the test were explained in detail prior to test commencement, and subject performance was monitored closely by a trained examiner during the test, and reinstructed if necessary. Pupil diameter was measured for the background mesopic and photopic conditions used, and also in the presence of both glare sources using a Neuroptics VIP-200 pupillometer (Neuroptics Inc., Irvine, Calif. 92612, USA).

(153) Photostress recovery time (PRT) of the short wavelength sensitive (SWS) visual system was assessed using a macular automated photostress (MAP) test, an adaptation of the Humphrey visual field analyzer (Model 745i Carl Zeiss Meditec Inc. Dublin, Calif., USA) for the assessment of foveal incremental light threshold (Dhalla et al., Am J Ophthalmol. 2007; 143(4), 596-600). To isolate SWS cones, mid and long wavelength sensitive cones were desensitized using a three minute sustained exposure to a 100 cd/m.sup.2, 570 nm bleaching background. A Goldmann V, 440 nm stimulus, presented for 200 milliseconds, was used to test the sensitivity of the SWS system before and after photostress. Following the three minute adaptation and practice session (during which subject performance was assessed for reliability and understanding), subjects were directed to fixate centrally between four circumferential light stimuli, and to respond to the detection of a blue stimulus at that location using the response button provided. Foveal sensitivity was determined as the average of three consecutive measurements recorded in decibels (dB), with each dB representing a 0.1 log unit sensitivity variation. Following baseline foveal sensitivity calculation, the subject was exposed to a short wavelength dominated photostress stimulus, which consisted of a 5-s exposure to a 300-W lamp viewed at 1 m through a low-pass glass dichroic filter, thus creating a temporary foveal blue after-image to mask fixation and reduce foveal sensitivity. Immediately post-photostress, a continuous and timed cycle of foveal sensitivity measurements were conducted and recorded. The reduction in foveal sensitivity from baseline, along with the recovery characteristics of the SWS system sensitivity, was recorded. Pupil diameter was again recorded for background light conditions, and in the presence of the photostress light source.

(154) Ocular straylight was measured using an Oculus C-Quant (OCULUS Optikgerte GmbH, Wetzlar, Germany), an instrument designed to quantify the effect of light scatter on vision. A central bipartite 14 test field was viewed monocularly through the instrument eyepiece. Subjects were instructed to respond, using the appropriate response button, to indicate the position of the most strongly flickering right or left test hemi-field. Subjects were allowed a defined practice session, during which reliable understanding of the task was assessed by the trained examiner. Test results were deemed acceptable only when the standard deviation of measured straylight value (esd) was 0.08, and the reliability coefficient (Q) was 1. Absolute straylight values were recorded in logarithmic form [log(s)].

(155) Visual discomfort was assessed during the glare disability and photostress testing procedures. Subjects were asked to rate their discomfort immediately following presentation of the glare and photostress light sources on a scale ranging from 1-10, where 1 indicated no ocular discomfort, 5 indicated moderate ocular discomfort, and 10 indicated unbearable ocular discomfort. Such a scale has previously been used effectively in an exemplar macular pigment/glare study (Stringham et al., Invest Ophthalmol Vis Sci. 2011; 52(10):7406-15). Visual experience was also assessed by questionnaire, using a modified version of the Visual Activities Questionnaire, as used and described in detail elsewhere (Loughman et al. Vis Res. 2010; 50:1249-1256; Sloane et al., The Visual Activities Questionnaire: Developing an instrument for assessing problems in everyday visual tasks. Technical Digest, Noninvasive Assessment of the Visual System, Topical Meeting of the Optical Society of America, January 1992). Iris color was also graded using a standardized iris classification scheme as defined by Seddon et al. (Invest Ophthalmol Vis Sci 1990 (31), 8:1592-1598).

(156) 3. BCVA demonstrated no significant effect for any of the intervention groups at 3 months. At 6 months, pair t-test analysis revealed a statistically significant improvement in BCVA compared to baseline for group 2(p=0.008). Repeated measures ANOVA confirmed a significant change across the three study visits for group 2 (p=0.034).

(157) 4. Contrast Sensitivity

(158) Mesopic and photopic contrast sensitivity improved from baseline values across a range of spatial frequencies at three months, and in particular, at six months. At three months, statistically significant improvements were noted at 1.5 cpd (p=0.008) for mesopic conditions, and at 3 cpd (p=0.024) and 12 cpd (p=0.025) for photopic conditions for Group 2. At six months, statistically significant improvements in CS were noted across a substantially broader set of spatial frequencies, most notably under mesopic conditions, for Group 2, Mesopic CS at 6 CPD improved significantly for Group 1 at 6 months (p<0.05). Repeated measures ANOVA confirms the improvements in contrast sensitivity to be statistically significant across all study visits for at least 3 of the 5 spatial frequencies tested under mesopic and photopic conditions. A detailed summary of contrast sensitivity results are provided in Table 16.

(159) TABLE-US-00020 TABLE 16 Contrast sensitivity change and significance levels at each spatial frequency tested under mesopic and photopic conditions Contrast sensitivity Contrast sensitivity T Test RM ANOVA Group Intervention at baseline at six months p* p** Photopic at 1.5 cpd Photopic at 1.5 cpd 20 mg L; 2 mg Z 44 26 53 20 0.05 0.12 10 mg MZ; 10 mg L; 2 mg Z 49 30 68 28 0.07 0.12 Placebo 52 22 62 29 0.41 0.28 Photopic at 3.0 cpd Photopic at 3.0 cpd 20 mg L; 2 mg Z 85 37 85 29 0.96 0.68 10 mg MZ; 10 mg L; 2 mg Z 73 25 100 28 0.002 0.002 Placebo 95 36 94 46 0.84 0.81 Photopic at 6.0 cpd Photopic at 6.0 cpd 20 mg L; 2 mg Z 99 27 100 28 0.71 0.43 10 mg MZ; 10 mg L; 2 mg Z 95 36 114 45 0.23 0.26 Placebo 103 54 116 64 0.83 0.88 Photopic at 12.0 cpd Photopic at 12.0 cpd 20 mg L; 2 mg Z 30 10 39 17 0.178 0.26 10 mg MZ; 10 mg L; 2 mg Z 32 13 50 30 0.011 0.008 Placebo 57 43 62 42 0.643 0.92 Photopic at 18.0 cpd Photopic at 18.0 cpd 20 mg L; 2 mg Z 8 5 12 9 0.168 0.38 10 mg MZ; 10 mg L; 2 mg Z 12 6 23 17 0.059 0.042 Placebo 20 17 17 14 0.527 0.73 Mesopic at 1.5 cpd Mesopic at 1.5 cpd 20 mg L; 2 mg Z 57 30 63 23 0.618 0.83 10 mg MZ; 10 mg L; 2 mg Z 52 18 76 24 0.003 0.000 Placebo 65 27 75 24 0.201 0.24 Mesopic at 3.0 cpd Mesopic at 3.0 cpd 20 mg L; 2 mg Z 78 45 74 35 0.792 0.91 10 mg MZ; 10 mg L; 2 mg Z 58 17 88 38 0.003 0.001 Placebo 68 39 96 44 0.101 0.11 Mesopic at 6.0 cpd Mesopic at 6.0 cpd 20 mg L; 2 mg Z 41 13 53 21 0.06 0.004 10 mg MZ; 10 mg L; 2 mg Z 50 19 77 49 0.14 0.058 Placebo 53 46 63 43 0.58 0.82 Mesopic at 12.0 cpd Mesopic at 12.0 cpd 20 mg L; 2 mg Z 7 4 9 6 0.198 0.16 10 mg MZ; 10 mg L; 2 mg Z 10 6 33 30 0.040 0.01 Placebo 13 14 21 25 0.400 0.50 Mesopic at 18.0 cpd Mesopic at 18.0 cpd 20 mg L; 2 mg Z 2 0 2 0 NS 0.17 10 mg MZ; 10 mg L; 2 mg Z 2 9 11 14 0.047 0.021 Placebo 4 5 5 3 0.593 0.28 RM ANOVARepeated measures ANOVA across all study visits; NSnon significant (statistic not computed as SE of difference = 0) *difference between baseline and 6 months (paired samples t test) **repeated measures ANOVA across all visits Group 1: n = 11; Group 2: n = 11; Group 3: n = 10

(160) 5. Glare Disability

(161) Mesopic and photopic glare disability improved from baseline across a range of spatial frequencies at three months and at six months. At three months, statistically significant improvements were noted at 12 cpd (p=0.048) for mesopic conditions, and at 1.5 cpd (p=0.023) and 3 cpd (p=0.033) for photopic conditions for Group 2. At six months, statistically significant improvements were noted across a substantially broader set of spatial frequencies for Group 2. Repeated measures ANOVA across all study visits reveals no statistically significant change, at any spatial frequency, in mesopic or photopic glare disability for Groups 1 and 3. The statistically significant improvements in glare disability for Group 2, under both mesopic and photopic conditions, for all spatial frequencies tested (other than 18 cpd) were robust to repeated measures ANOVA. A detailed summary of glare disability results are provided in Table 17.

(162) TABLE-US-00021 TABLE 17 Glare disability change and significance levels at each spatial frequency tested under mesopic and photopic conditions Glare Disability Glare Disability T test RM ANOVA Group Intervention at baseline at six months p* p** Photopic at 1.5 cpd Photopic at 1.5 cpd Group 1: 20 mg L; 2 mg Z 56 27 67 20 0.056 0.12 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 50 22 67 22 0.059 0.033 Group 3: Placebo 60 25 74 29 0.134 0.24 Photopic at 3.0 cpd Photopic at 3.0 cpd Group 1: 20 mg L; 2 mg Z 84 26 95 31 0.175 0.28 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 86 24 121 34 0.003 0.002 Group 3: Placebo 96 30 97 44 0.964 0.92 Photopic at 6.0 cpd Photopic at 6.0 cpd Group 1: 20 mg L; 2 mg Z 114 43 96 37 0.181 0.26 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 91 39 130 40 0.032 0.04 Group 3: Placebo 105 51 112 58 0.644 0.80 Photopic at 12.0 cpd Photopic at 12.0 cpd Group 1: 20 mg L; 2 mg Z 34 13 32 14 0.785 0.13 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 42 20 70 25 0.004 0.006 Group 3: Placebo 29 21 62 48 0.06 0.13 Photopic at 18.0 cpd Photopic at 18.0 cpd Group 1: 20 mg L; 2 mg Z 17 11 23 12 0.35 0.08 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 33 13 65 20 0.17 0.23 Group 3: Placebo 33 15 46 22 0.41 0.75 Mesopic at 1.5 cpd Mesopic at 1.5 cpd Group 1: 20 mg L; 2 mg Z 23 8 45 35 0.08 0.05 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 39 26 58 29 0.08 0.04 Group 3: Placebo 32 24 38 23 0.76 0.25 Mesopic at 3.0 cpd Mesopic at 3.0 cpd Group 1: 20 mg L; 2 mg Z 36 10 61 43 0.066 0.06 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 40 14 74 40 0.009 0.02 Group 3: Placebo 54 39 59 46 0.820 0.93 Mesopic at 6 cpd Mesopic at 6 cpd Group 1: 20 mg L; 2 mg Z 64 41 90 53 0.15 0.17 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 50 19 77 49 0.07 0.049 Group 3: Placebo 53 46 64 43 0.66 0.71 Mesopic at 12 cpd Mesopic at 12 cpd Group 1: 20 mg L; 2 mg Z 5 2 10 17 0.303 0.35 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 5 2 12 8 0.016 0.014 Group 3: Placebo 7 5 10 7 0.238 0.15 Mesopic at 18 cpd Mesopic at 18 cpd Group 1: 20 mg L; 2 mg Z 2 0 2 0 0.34 0.44 Group 2: 10 mg MZ; 10 mg L; 2 mg Z 2 1 11 13 0.16 0.21 Group 3: Placebo 4 5 5 3 0.14 0.22 cpdCycles per degree *difference between baseline and 6 months (paired samples t test) **repeated measures ANOVA across all visits Group 1: n = 11; Group 2: n = 11; Group 3: n = 10

(163) 6. Photostress Recovery Time

(164) Photostress recovery time did not improve significantly for any of the Groups during the study period (p>0.05 for all). Paired t test analysis revealed, however, that the improvement in PRT for Group 2 (PRT 37 seconds [or 21%] shorter on average at six months compared to baseline) approached, but did not reach statistical significance (t=2.067, p=0.069).

(165) Ocular straylight measures did not change significantly for any Group (p>0.05 for all). Visual experience and ocular discomfort, as determined by questionnaire and discomfort rating, did not change significantly during the study period for any Group.

(166) 7. Comparison of Changes in MP with Changes in Visual Performance Parameters

(167) A comparison was made between changes in macular pigment and changes in visual performance parameters between baseline and 6 months. There was no statistically significant relationship between change in macular pigment and any of the visual performance variables (p>0.05 for all). Table 18 gives the results for photopic (daytime) and mesopic (night-time) contrast sensitivity at 1.5 cpd.

(168) TABLE-US-00022 TABLE 18 Changes in macular pigment (at 0.25 eccentricity) compared with changes in the following visual performance parameters between baseline and 6 months: BCVA, photopic (daytime) contrast sensitivity, mesopic (night-time) contrast sensitivity, photopic contrast sensitivity under glare conditions, mesopic contrast sensitivity under glare conditions. r p Change in MP vs change in BCVA Group 1 (20 L, 2 Z) 0.075 0.827 Group 2 (10 L, 10 MZ, 2 Z) 0.09 0.794 Group 3 (placebo) 0.119 0.743 Change in MP vs change in photopic CS (1.5 cpd) Group 1 (20 L, 2 Z) 0.104 0.76 Group 2 (10 L, 10 MZ, 2 Z) 0.154 0.651 Group 3 (placebo) 0.242 0.5 Change in MP vs change in mesopic CS (1.5 cpd) Group 1 (20 L, 2 Z) 0.394 0.231 Group 2 (10 L, 10 MZ, 2 Z) 0.082 0.81 Group 3 (placebo) 0.179 0.621 Change in MP vs change in photopic GD (1.5 cpd) Group 1 (20 L, 2 Z) 0.348 0.294 Group 2 (10 L, 10 MZ, 2 Z) 0.263 0.435 Group 3 (placebo) 0.331 0.351 Change in MP vs change in mesopic GD (1.5 cpd) Group 1 (20 L, 2 Z) 0.394 0.231 Group 2 (10 L, 10 MZ, 2 Z) 0.082 0.81 Group 3 (placebo) 0.179 0.621 Abbreviations: MP = macular pigment; BCVA = best corrected visual acuity; L = lutein; Z = zeaxanthin; MZ = meso-zeaxanthin; CS = contrast sensitivity; cpd = cycles per degree; GD = glare disability.

(169) Surprisingly, these data show that the observed increases in visual performance parameters were independent of the increases in macular pigment.

(170) Discussion

(171) In terms of MPOD, there was no significant change at any eccentricity, at 3 or at 6 months, in subjects supplemented with a preparation that does not contain MZ or in subjects given placebo. In contrast, subjects supplemented with all three macular carotenoids exhibited a significant increase in MPOD at 4 of the 5 eccentricities tested, at 3 months and at 6 months.

(172) The current study demonstrates a novel and important effect of MP augmentation on visual performance among healthy subjects without ocular disease. Across a broad range of testing modalities and conditions, visual performance improved significantly among subjects who exhibited a significant rise in MPOD. Specifically, improvements in contrast sensitivity and glare disability (across virtually all spatial frequencies, and under daytime and nighttime conditions), and improvements in visual acuity, were demonstrated in subjects supplement with all three macular carotenoids, but no such observations were seen in the placebo control subjects or in subjects supplemented with L and Z (but not MZ).

(173) The data support the view that MP may influence visual performance through its optical filtration effects, as the glare disability test protocol included an LED glare source that exhibited a short wavelength peak emission profile matching the known spectral absorbance of MP. The observed improvements in acuity and contrast sensitivity, however, are less consistent with a solely optical explanation. The stimuli used do, however, contain a relatively small short wavelength component. It is possible, therefore, that MP augmentation results in optical image enhancement through a reduction of the deleterious effects of chromatic aberration and light scatter, and thereby improves visual acuity and contrast sensitivity, even for such spectrally broadband stimuli. It is also possible that the macular carotenoids, which are intracellular compounds, also play a neurobiological role, thereby contributing to, and/or facilitating, optimal neurophysiological performance, and hence visual function (the limits of spatial vision represent the combined influence of optical and neural efficiency limits). This view is supported by observation that there was no correlation between increases in visual performance and increases in macular pigment, suggesting that the MP carotenoids may exert effects on visual performance by a neuro-physiological mechanism.

(174) In conclusion, we have demonstrated a rapid and sustained rise in MPOD following supplementation with all three macular carotenoids, and this was not observed in placebo-controlled subjects or in subjects supplemented with a preparation lacking MZ. Further, supplementation with all three macular carotenoids resulted in significant improvements in contrast sensitivity and glare disability (under photopic and scotopic conditions) and in corrected distance visual acuity, whereas no such changes were seen in placebo controls or in subjects supplemented with a preparation lacking MZ. These findings have potentially important implications for people engaged in activities where optimization of visual importance is important (especially if operating under bright conditions), and warrant further study.

Example 5

Effect of a Supplement Containing MZ on Visual Performance in Subjects with an Atypical Distribution of Macular Pigment (a Central Dip)

(175) Subjects and Dosage

(176) Eight subjects with pre-identified central dips in their macular pigment spatial profile as described in example 2 were recruited into this study. All eight subjects consumed a supplement containing 10 mg L, 10 mg MZ, and 10 mg Z daily for 3 months.

(177) Methods

(178) Macular pigment optical density (MPOD) was measured as in Example 1 at baseline and after 3 months of MZ supplementation. Letter contrast sensitivity (Thomson Chart) was likewise measured using the method described in Example 3 section 4

(179) Results 1. MPOD results: As seen from Table 19 and FIG. 10, the spatial profile of MP was normalised following supplementation with 10 mg L, 10 mg MZ, and 10 mg Z for 3 months. All subjects responded to this intervention. Statistically significant increases were seen at all eccentricities except for 0.5.

(180) TABLE-US-00023 TABLE 19 Eccentricity Baseline 3 months p 0.25 0.51 0.25 0.64 0.21 <0.001 0.5 0.54 0.25 0.57 0.20 0.140 1 0.37 0.20 0.43 0.21 0.016 1.75 0.20 0.12 0.26 0.12 0.008 2. Contrast sensitivity: as seen from Table 20 there was an improvement in contrast sensitivity following supplementation with 10 mg L, 10 mg MZ, and 10 mg Z for 3 months.

(181) TABLE-US-00024 TABLE 20 Contrast sensitivity Baseline 3 months p 1.2 cpd 2.00 0.15 2.07 0.12 0.103 2.4 cpd 1.86 0.16 2.02 0.19 0.003 6 cpd 1.56 0.19 1.71 0.21 <0.001 9.6 cpd 1.34 0.21 1.46 0.18 0.051 15.15 cpd 1.02 0.16 1.11 0.20 0.035

Example 6

(182) In one embodiment, the composition of the invention takes the form of a mineral- and vitamin-containing dietary supplement, augmented with MZ, L and, optionally Z. The supplement is formulated as a tablet, with the following composition of active ingredients:

(183) TABLE-US-00025 MZ 5 mg L 5 mg Z 1 mg Vitamin A 800 micrograms Thiamin 1.1 mg Riboflavin 1.4 mg Vitamin B6 2.0 mg Vitamin B12 2.5 micrograms Folic acid 400 micrograms Niacin 20 mg Pantothenic Acid 6 mg Biotin 50 micrograms Vitamin C 80 mg Vitamin D 20 micrograms Vitamin E 12 mg Calcium 120 mg Magnesium 60 mg Iron 14 mg Zinc 10 mg Copper 1 mg Iodine 150 micrograms Manganese 3 mg Chromium 40 micrograms Selenium 55 micrograms Molybdenum 50 micrograms

(184) The following ingredients may be used as a source of the minerals and vitamins.

(185) Minerals: calcium carbonate, magnesium hydroxide, ferrous fumarate, zinc oxide, copper sulphate, potassium iodide, manganese sulphate, chromic chloride, sodium selenate, sodium molybdate

(186) Vitamins: Retinyl acetate, Thiamin mono nitrate, Riboflavin, Pyridoxin hydrochloride, Cyano cobalomin, Folic Acid, Niacin, Calciun-D-pantothenate, D-biotin, Sodium Ascorbate#, Cholecalciferol, D-alpha-tocopherol acetate

(187) The tablets may conveniently additionally comprise one or more of the following fillers: Malto dextrin, Microcellulose, Hydroxy propyl methyl cellulose, Shellac, Talcum, Gum acacia, Glycerol, Titanium dioxide, Polyfructose

(188) One tablet (e.g. 500 mg) to be taken per day.

Example 7

Provision of MZ in Egg Yolks for Human Consumption

(189) Several workers have shown that uptake of L and Z from egg yolks is 2-4 times more efficient than from capsules (Handleman et al, 1999 Am. J. Clin. Nutr. 70, 247-251; Goodrow et al., 2006 J. Nutr. 136, 2519-2524; Johnson 2004, J. Nutr. 134, 1887-1893).

(190) The objective of this study was to feed hens a mixture of L, MZ and Z to determine the total amount of MZ in the yolk. In addition 24 eggs collected at the end of the experiment were consumed by one subject, one egg/day and the blood MZ composition determined.

(191) Methods

(192) Eight Bovan Goldline hens were obtained at approximately 18 weeks of age.

(193) When the hens were producing at least 8 eggs per day in total, the hens were isolated and fed only a commercial meal. The experiment was started 1 week later when a premix containing the mixed carotenoids was added to the meal. The premix provided 250 mg MZ/kg feed with proportions of L 50, MZ 30, Z 20.

(194) The yolk carotenoids were measured in mixtures prepared from all eggs collected at baseline, three and six weeks.

(195) Preparation of Egg-Yolk Suspensions

(196) Yolks were individually weighed and mixed with phosphate-buffered saline and made up to 50 ml. Two ml of each suspension was mixed in a separate universal tube for each of the three batches separately and stored at 40 C.

(197) Carotenoid Extraction

(198) (i) Egg Yolk Suspensions

(199) The egg yolk suspension (0.1 ml) was mixed with 0.15 ml aqueous KOH (25 gl100 mlwater), 0.15 ml absolute ethyl alcohol and 0.1 ml echinenone (internal standard, 0.4 mg/500 ml ethyl alcohol) in a glass extraction tube and incubated at 45 C for 45 minutes.

(200) Solutions were then cooled and mixed vigorously with 1.5 ml hexane (containing BHT500 mg/l) and centrifuged to separate the hexane and aqueous layers. One ml of the upper hexane layer was transferred to an evaporating tube and the residue was re-extracted with 1.5 ml hexane. After centrifuging, 1.5 ml of the upper layer was removed and the extracts combined and evaporated to dryness under nitrogen at 40 C. The residue was made up to 0.15 ml with mobile phase (see Ultracarb HPLC below) and 0.1 ml was injected onto a Ultra Carb Column for HLPC analysis.

(201) (ii) Plasma

(202) Blood (10 ml) from a human subject was collected in lithium heparin tubes at baseline, day 12 and day 24 after consumption of one egg per day and centrifuged to provide plasma subsequently stored at 40 C. Plasma, 0.25 ml was mixed with 0.2 ml sodium dodecyl sulphate, 0.4 ml ethyl acetate (internal standard). Hexane containing BHT (1.0 ml) was added and the mixture extracted vigorously for 4 minutes, centrifuged for 10 mins and 0.7 ml of the upper hexane layer removed and evaporated to dryness.

(203) The residue was made up to 0.1 ml with mobile phase (see HPLC procedure below) and 0.05 ml was injected onto the column.

(204) Liquid Chromatography (HPLC) to Measure L, MZ, Z

(205) Separation and quantitation of the MZ was achieved using a two column procedure.

(206) Ultracarb procedure: Extracts prepared as described above were reconstituted in a mobile phase comprising acetonitrile:methanol (85:15 containing 0.1% triethylamine). Using the same solvent mixture at 1.5 ml/min, extracts were chromatographed isocratically using a 3 micro m Ultracarb ODS column (2504.6 mm, Phenomenex, UK) and detected using a photodiode-array detector (model 2996, Waters Ltd) to quantify L and Z+MZ at 450 nm. Eluent that coincided with the emergence of MZ+Z was collected from the waste line and evaporated to dryness under nitrogen.

(207) Chiral chromoatography: The Z+MZ extract was then reconstituted in 0.1 ml of hexane:isopropanol (90:10) and 50 uL was chromatographed on a 10 micro m Chiralpak AD column (2504.6 mm; Chiral Technologies Europe, 67404 Illkirch Cedex, France) to determine the proportion of MZ and Z isomers using gradient elution at 0.8 ml/min starting with 90% hexane and 10% isopropyl alcohol and increasing to 95% hexane in a linear gradient over 30 minutes.

(208) Results

(209) MZ in Egg Yolks

(210) Mean (SD) weights of the yolks at baseline and at the end of weeks 3 and 6 were 12.29 (0.35), 14.23 (0.87) and 15.73 (0.72) g respectively. The MZ contents of the yolks are shown in Table 21. At baseline only L and Z were present;

(211) Feeding 250 ppm of the carotenoid mixture for 3 weeks produced egg yolks containing 2.78 mg MZ/yolk of which L was circa 76% Z 13% and MZ 11%. There was no further increase at 6 weeks

(212) Plasma

(213) The MZ content in plasma from one human subject consuming one egg per day are shown in table 22.

(214) Baseline total MZ concentration was 0.81 micro mol/liter of which L was 53% Z was 47% and MZ 0%. The concentration of L had almost trebled at day 12 but the concentration then fell to only double the baseline value at day 24.

(215) The increase in MZ+Z at days 12 and 24 was 30% and 23% respectively and was due solely due to increase in MZ.

(216) Conclusions

(217) Feeding a mixture of carotenoids to chickens for 3 and 6 weeks increased L+MZ+Z in the egg yolks and in plasma in a subject consuming one egg per day.

(218) The MZ content per yolk was raised from circa 0.8 mg to 2.8 mg. Since it is known that the absorption of L and Z from egg yolk is enhanced, two or three eggs from chickens fed a mixture of L, Z and MZ could provide sufficient MZ to improve visual performance in the subject, although this was not tested.

(219) TABLE-US-00026 TABLE 21 MZ contents of egg yolks from chicken fed 250 mg/kg mixed carotenoids micro grams per yolk Weeks L Z MZ Total 0 563 278 0 841 3 2100 366 315 2781 6 2260 328 272 2860

(220) TABLE-US-00027 TABLE 22 MZ contents of plasma in one person consuming one egg per day (units are micro moles per litre). Day L Z MZ Total 0 0.55 0.26 0 0.81 12 1.20 0.28 0.06 1.54 24 1.06 0.25 0.07 1.38

Example 8

The Addition of MZ to Dietary Formulations and VP

(221) A dry powder formula dietary supplement composition can be prepared by mixing 5 mg MZ, 5 mg L and 1 mg Z with the contents of 4 sachets containing circa 50 g each of The Cambridge Diet product, obtained from Cambridge Nutritional Foods Limited, Stafford House, Brakey Road, Corby NN17 5LU, United Kingdom (The Cambridge Diet is a registered trade mark).

Example 9

Fish Oils, MZ and VP

(222) The retina contain a high concentration of Omega 3 fatty acids which are especially abundant in fish oils, for example oils from salmon, herring, mackerel, anchovies, sardines; also from krill and green-lipped muscles. Omega 3 fatty acids are found as eicosapentanoic acid C22.6n3 (EPA) and docosahexanoic acid C22.6n3 (DHA) and combined make up about 30% of fish body oil. The acceptable daily macro nutrient dose (AMND) of EPA+DHA is about 1.6 g/day for men and 1.1 g/day for women, i.e. about 5 g and 3.5 g fish oil respectively.

(223) The occurrence of a high concentration of omega 3 fatty acids in the retina suggests that they may play and important role in vision. A combination of macular carotenoids (MC) containing MZ with omega3 fatty acids would thus be beneficial to the retina and improve visual performance. The mixture can be in capsules or as an emulsion in a sachet. The latter has the advantage that fewer doses can be given in a sachet whilst several large capsules (which many elderly people find difficult to swallow) are needed for the AMND. The emulsion can contain from 25-60% fish oils to provide from 0.5-2.0 g omega-3 fatty acids and sufficient MC to give a daily dose of 0.5 mg to 50 mg MC per day.

(224) A commercial preparation of active macular carotenoids (MC) consisting of mesozeazanthin 10 g lutein 10 g and zeaxanthin 2 g in 78 ml krill oil is mixed with 900 ml salmon oil and made into soft gel capsules each containing 1 g oil formulation. A daily dose of 5 capsules will provide the 1.5 g of Omega 3 fatty acids and a 22 mg dose of macular carotenoids to improve visual performance.

(225) An alternative embodiment may be formulated as follows:

(226) TABLE-US-00028 Ingredients The mixture of and MC, krill and salmon oils as above 55% Water 35% Sucralose (Splenda) 4% Milk powder 5% Potassium sorbate 0.1% Alpha tocopherol 0.1% Flavorings (e.g. citrus) 0.8%

(227) An emulsion is made under an inert atmosphere using standard techniques and then packed into airtight sachets each containing 5 grams emulsion. The daily dose is 2 sachets per day containing 6 g omega 3 fatty acids and 22 mg MC.