Method for coating synthetic polymer surface with biopolymer

Abstract

An object to be solved by the present invention is to provide a method for preparing a composition prepared by firmly coating the surface of a material with a biopolymer, and a composition prepared by this method wherein the surface of a material is firmly coated with a biopolymer. The present invention provides a method for coating with a biopolymer, which comprises coating the surface of a synthetic polymer with a biopolymer dissolved in a solvent comprising an organic fluorine compound.

Claims

1. A method for coating with a biopolymer, which comprises coating the surface of a synthetic polymer with a solution of a biopolymer dissolved in a solvent comprising an organic fluorine compound to prepare a composition, whereby the surface of the synthetic polymer is partially dissolved by the organic fluorine compound, wherein: the synthetic polymer is a polymer which is used as medical material and is embedded within a living organism; in the composition, the ratio of the biopolymer to the synthetic polymer continuously decreases depending on the depth from a surface of the composition; the surface of the synthetic polymer is coated with the biopolymer without causing chemical changes due to introduction of chemical bonds; the biopolymer is gelatin or albumin; the organic fluorine compound is 1,1,1-hexafluoro-2-propanol, trifluoroethanol, hexafluoroacetone, trifluoroacetic acid, or pentafluoropropionic acid; and the synthetic polymer contains at least one selected from the group consisting of polylactic acid, polyglycolic acid, polyethylene terephthalate, and polyurethane.

2. The method according to claim 1, wherein the protein is a human-, bovine-, pig-, or fish-derived protein, or a gene recombinant protein.

Description

EXAMPLES

Example 1

Albumin Coating of PET Film

(1) PET film (2 cm2 cm) was coated with an aqueous solution (400 L) containing 0.1% albumin or a solution (400 L) of 1,1,1-hexafluoro-2-propanol (HFIP), followed by 1 day of natural drying. The film was immersed in water at 37 C. and then shaken for 1 hour. The sweepback contact angle of water of the film was measured. The sweepback contact angle in the case of the film prepared by coating with the aqueous solution was 655 degrees which is similar to that in the case of untreated PET film (untreated PET film: 684 degrees). On the other hand, the sweepback contact angle of water of the film prepared by coating with the HFIP solution was significantly decreased to be 116 degrees. It can be said that albumin coating was effectively achieved on the PET film with the use of HFIP as a solvent for coating. Furthermore, HFIP partially dissolved the PET surface, so that the concentration of albumin to PET was continuously decreased.

Example 2

Gelatin Coating of PET Film

(2) Gelatin coating of PET film was carried out by using the process used in Example 1. Similar to Example 1, when water was used as a solvent for coating, the sweepback contact angle of water after washing with water was found to be similar to that of an untreated case. On the other hand, when trifluoroethanol was used as a solvent, the sweepback contact angle of water was significantly decreased to be 136 degrees. It can be said that PET could be effectively coated with gelatin with the use of TFE as a solvent for coating.

INDUSTRIAL APPLICABILITY

(3) According to the present invention, the surface of a synthetic polymer can be firmly coated with a biopolymer without causing chemical changes due to introduction of chemical bonds.