GAS SENSOR

Abstract

We describe a method of selectively detecting the presence of an analyte. The method comprises providing a waveguide with a core comprising porous material; absorbing an analyte sample into the porous material of the core such that the analyte sample is held within pores of the core; waveguiding radiation along the waveguide to an output; measuring spectral features of the output radiation due to absorption or scattering of said waveguided radiation by the absorbed analyte sample; and selectively identifying the presence of a target analyte in the sample from the spectral features. In embodiments spectral features are measured for multiple different waveguide core regions having different physical/chemical properties modified to provide additional selectivity to the target analyte(s), and these measurements are combined to identify the target analyte.

Claims

1. A method of selectively detecting the presence of an analyte, the method comprising: providing at least one waveguide, the waveguide having a core comprising porous material; absorbing an analyte sample into said porous material of said core such that said analyte sample is held within pores of said core; waveguiding radiation along said at least one waveguide to an output to provide output radiation; measuring one or more spectral features of said absorbed analyte sample in said output radiation due to absorption or scattering of said waveguided radiation by said absorbed analyte sample; selectively identifying the presence of a target analyte in said analyte sample from said one or more spectral features; wherein molecular absorption or Raman spectroscopy is performed on said analyte sample.

2. A method as claimed in claim 1 wherein said one or more spectral features are enhanced by capillary condensation.

3. A method as claimed in claim 1 further comprising providing a plurality of waveguide core regions with a plurality of different physical and/or chemical modifications to said core, wherein said plurality of waveguide core regions comprise one or both of a plurality of core regions of a plurality of said waveguides and a plurality of core regions of a plurality of portions of said at least one waveguide; waveguiding radiation through said plurality of waveguide core regions; measuring one or more said spectral features for each of said differently modified core regions; and selectively identifying said target analyte from a combination of said spectral features for said differently modified core regions.

4. A method as claimed in claim 3 wherein said differently modified core regions have pores with size distributions having peaks at different pore sizes.

5. A method as claimed in claim 3 or wherein said differently modified on regions comprise different functionalisations of said core material.

6. A method as claimed in claim 3, wherein said selective identifying uses a multi-component analysis of said combination of spectral features.

7. A method as claimed in claim 1 further comprising operating at a plurality of temperatures of said at least one waveguide, and measuring said one or more spectral features at said plurality of temperatures; wherein said selective identifying is responsive to said one or more spectral features at said plurality of temperatures.

8. A method as claimed in claim 7 wherein said temperatures are selected to change a degree of vapour condensation of said target analyte within said pores of said core.

9. A method as claimed in claim 1 wherein said measuring comprises waveguiding multiband radiation along said waveguide and measuring a spectrum of said output radiation.

10. A method as claimed in claim 1 wherein said pores have a size distribution with a peak at less than 500 nm.

11. A method as claimed in claim 1 comprising providing said waveguide on substrate and using porous silicon or porous silica for said core.

12. An analyte sensor, the sensor comprising: a substrate bearing a waveguide, the waveguide comprising: a first, cladding layer on said substrate; a second, core layer, comprising porous material, over said cladding layer; a radiation source to provide radiation into said waveguide; a radiation detector to detect radiation which has been waveguided along said waveguide; and a signal processor, coupled to said radiation detector, to identify the presence of a target analyte absorbed within said core layer from one or more spectral features of said detected radiation due to absorption or scattering of said waveguided radiation by said absorbed target analyte.

13. An analyte sensor as claimed in claim 12 wherein said core layer and said cladding layer each comprise a porous silicon-based material.

14. A method of fabricating an analyte sensor waveguide as claimed in claim 13, the method comprising: providing a silicon substrate; supplying a first current at a first current density perpendicular to said substrate; performing a first etch of said substrate to fabricate said core layer; and supplying a second current at a second density slowly perpendicular to said substrate; performing a second etch of said substrate to fabricate said core layer.

15. A method of fabricating an analyte sensor, comprising fabricating a waveguide as claimed in claimed 14, and then fabricating said analyte sensor using said waveguide.

16. Apparatus for selectively detecting the presence of an analyte, the apparatus comprising: at least one waveguide, the waveguide having a core comprising porous material; a radiation source to provide radiation into said waveguide; a radiation detector to detect radiation which has been waveguided along said waveguide; and a signal processor, coupled to said radiation detector, to identify the presence of a target analyte absorbed within said core layer from one or more spectral features of said target analyte in said detected radiation due to absorption or scattering of said waveguided radiation by said absorbed target analyte.

17. Apparatus as claimed in claim 16 comprising a plurality of waveguide core regions with a plurality of different physical and/or chemical modifications to said core; wherein said plurality of waveguide core regions comprise one or both of a plurality of core regions of a plurality of said waveguides and a plurality of core regions of a plurality of portions of said at least one waveguide.

18. A method as claimed in claim 17 wherein said signal processor is configured to selectively identify said target analyte from a combination of said spectral features for said differently modified core regions.

19. A method as claimed in claim 16, further comprising a temperature controller to control a temperature of said at least one waveguide to a plurality of different temperatures; and wherein said signal processor is configured to selectively identify said target analyte by measuring said one or more spectral features at said plurality of different temperatures.

20. A method of analysing a bodily fluid sample using the method of claim 1.

21. Apparatus as claimed in claim 16 for analysing a bodily fluid sample, the apparatus further comprising a bodily fluid sample holder, and means to selectively allow passage of bodily fluid vapour from the sample holder for analysis.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0035] These and other aspects of the invention will now be further described, by way of example only, with reference to the accompanying figures in which:

[0036] FIG. 1 shows a schematic illustration of three different waveguide types and their interactions with molecules, showing (a) a conventional waveguide, (b) a slot-waveguide and (c) a porous waveguide according to an embodiment of the invention; the target analyte molecules are represented as dots and the diffuse circles represent the propagating mode;

[0037] FIG. 2 illustrates absorption of light by molecules, showing that for the same physical interaction length, a nanoporous waveguide (lower figure; dispersed molecules, small interaction length, small absorption peak) provides significantly higher effective interaction length than with a gas (upper figure; molecules condensed within the material of the waveguide core, large effective interaction length, strong absorption peak), thus increasing the sensitivity of detection;

[0038] FIG. 3 shows the dynamic response of a single porous silicon layer for hexane vapour (left) and ethyl acetate vapour (right) at various concentrations, showing a measure of physical absorption of the vapour by the porous silicon on the y-axis;

[0039] FIGS. 4a to 4c show, respectively, the structure of an example fabricated PSi (porous silicon) waveguide comprising a porous core layer on a cladding layer, with inset HR-SEM (high resolution scanning electron microscope) images of the top surface and a cross-section view of the pore morphology (the scale bars are 100 nm); a first variant waveguide structure; and second variant waveguide structure without substrate or cladding;

[0040] FIG. 5 shows a vertical cross-section of a porous waveguide array (individual waveguides indicated with arrows), showing the emitted light from two adjacent waveguides (FIGS. 5a, 5b), and light propagation along a waveguide (FIG. 5c);

[0041] FIG. 6 shows, schematically, experimental apparatus used to characterise a sensor according to an embodiment of the invention;

[0042] FIGS. 7a to 7d show example measured spectra from the apparatus of FIG. 6 for, respectively, pentane, methanol, acetone and isopropyl alcohol;

[0043] FIGS. 8a to 8d show analyte sensing systems according to embodiments of the invention;

[0044] FIG. 9 shows a vertical cross-section through a temperature controlled porous waveguide sensor according to an embodiment of the invention; and

[0045] FIG. 10 shows an example of an analyte sensing probe comprising a porous waveguide according to an embodiment of the invention.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

[0046] We will describe techniques for optical spectroscopy of analytes in the pores of a porous waveguide. Some initial background material is helpful for understanding the invention:

Spectroscopy

[0047] Optical absorption spectroscopy is based on illuminating a chemical compound with a light and measuring the light absorption as a result of a presence of that chemical compound. Different molecules absorb light of different wavelengths. An absorption spectrum will show a number of absorption bands corresponding to structural groups within the molecule.

[0048] Absorbance is directly proportional to the path length, b, and the concentration, c, of the absorbing species. Beer's Law states that A=cbc, where is a constant of proportionality, called the absorbtivity.

[0049] The radiation can be of any wavelength ranging from the UV to IR and even THz.

Porous Silicon

[0050] Porous silicon is typically fabricated using electrochemical etching. The porosity of the fabricated layer depends on several parameters (wafer doping, temp, solution) and the applied current density during the electrochemical etching. The refractive index of a porous layer depends on the porosity, and therefore can be easily controlled. This enables easy fabrication of optical components such as Bragg reflectors, multilayers structures and waveguides.

[0051] In addition, the porous nature of the material can be used to host other optical materials, nanoparticles, chemical molecules and dyes.

Waveguides

[0052] An optical waveguide is a physical structure that guides electromagnetic waves, i.e. the electromagnetic waves propagate within the boundaries of the structure. Optical waveguides can be classified according to their geometry (planar, strip, or fibre waveguides), mode structure (single-mode, multi-mode), refractive index distribution (step or gradient index) and material (glass, polymer, semiconductor). As the skilled person will appreciate, a waveguide generally comprises a core with a cladding (which may be gas/air/vacuum) of lower refractive index.

Capillary Condensation

[0053] The number of molecules that can be adsorbed by a porous layer is limited by the total pore volume. The pores' curved surfaces enhance the attraction for molecules of wetting substances due to van der Waals interactions, resulting in adsorption and capillary condensation. Capillary condensation is the physical tendency for a vapour to condense in a small pore at temperatures well above the dew point, and is described by the Kelvin equation:

[00001]$N_A.Math.\mathrm{kT}.Math..Math.\ln \left(\frac{P}{P_0}\right)=-\frac{2.Math..Math.M.Math..Math..Math..Math.\cos .Math..Math.}{r.Math..Math.}$

where N.sub.A is Avogadro's constant, k is the Boltzmann constant, T is the absolute temperature, M is the molecular weight, is the liquid density, r is the radius of the capillary, is the contact angle, is the surface tension, P.sub.0 is the saturation pressure and P is the equilibrium vapour pressure. Therefore, the smaller the pore radius, the lower is the relative vapour pressure at which capillary-condensation can occur at a given temperature. During the capillary-condensation process, the air in the voids is replaced by condensed vapour. The extent of both the monolayer adsorption and the capillary condensation are influenced by the surface affinity of the porous matrix, which can be tailored by a range of chemical modifications.

Thermal Modulation

[0054] For a single sensor, using thermal modulation, can help discriminate between different vapours. When the sensor working temperature is modulated, the kinetics of the adsorption and reaction processes that occur on the sensor surface are altered. This leads to sensor response that is characteristic to species present, thus increased selectivity.

Porous Waveguide Sensing

[0055] Embodiments of the invention relate to the use of a porous waveguide with sub-wavelengths features with a light source and detector to measure molecular absorption spectroscopy of species entering the pores of the waveguide. The porous waveguide can be made of any material and may be fabricated in many different ways. Pore and nano-features dimensions are smaller than the wavelength of light, where Rayleigh scattering losses (which vary as .sup.4) are expected to be low enough to allow light to propagate in the waveguide. The preferred waveguide design we describe facilitates easy access of analytes into the pores (by contrast with buried waveguides made of porous silicon). The detection optionally employs the capillary condensation phenomenon to facilitate further discrimination of analytes that enter the pores.

[0056] Current optical (bio-)sensors are limited in their sensitivity because the chemical interactions are limited to the surface of the waveguiding device, while the high-intensity propagating mode is localised in the core. Increasing the interaction between the propagating light and the target molecules, as well as increasing the surface area, results in increased sensitivity and better SNR.

[0057] In FIG. 1, three different types of waveguides are compared. FIG. 1a shows a conventional waveguide where the light propagates in the core and only the evanescent field interacts with the molecules of interest (dots). One way to improve the sensitivity is to add a small slot inside the core (FIG. 1b). This slot is very thin (smaller than the wavelength of light) in order for the mode to propagate normally in the structure. This adds to the complexity of fabrication, and also the slot is limited in the number of molecules that it can accommodate. A porous waveguide (FIG. 1c) comprises many small pores, with the ability to accommodate large quantity of molecules. The target molecules are absorbed within the waveguide core, thus providing very sensitive detection. Due to the small size of the pores (much smaller than the wavelength of light), the light can propagate in the porous core in a similar manner to a conventional waveguide.

[0058] In addition to the large surface area which is generally desirable by a sensor, the porous network offers additional advantages: The size of the pores can be tuned. This can provide physical discrimination based on sizefor example only the smaller molecules may be measured. The surface may be treated to become hydrophilic, hydrophobic or may be tailored by a range of chemical modifications. The surface may be functionalized with receptors to allow specific binding with the target molecules (DNA, antibody).

[0059] In the gas phase, the nanostructured optical waveguide significantly increases the sensitivity of detection for volatile organic compounds (VOCs) due to a capillary condensation phenomenon: Capillary condensation is the physical tendency for a vapour to condense in a small pore at temperatures well above the dew point (as described by the Kelvin equation). During the capillary-condensation process, the air in the voids is replaced by condensed vapour. The extent of the condensation is influenced by the surface affinity of the porous matrix to the vapour molecules and depends on the vapour intrinsic properties. The molecules in the liquid are in equilibrium with the gaseous molecules, and the process is reversible: as the gas concentration decreases, the molecules leave the pores. The nanostructured optical waveguide acts as a condenser or concentrator of organic volatiles. FIG. 2 is a schematic illustration showing the role of the nanostructured waveguide: Conventionally, the light interacts with gas molecules which are dispersed in air. However when a nano-porous waveguide is introduced, the gas molecules condense inside the waveguide, providing a significantly larger effective interaction length.

[0060] The dynamic curves of adsorption, condensation and desorption are different for different vapours. FIG. 3 shows the real-time sensor response for 5 injections of two different vapours. The adsorption and desorption of vapour into and out of the porous structure is fast and fully reversible (albeit with some hysteresis).

[0061] We now describe some further methods of improving the reliability and the selectivity of this approach: Temperature modulation provides further discriminationthe capillary condensation and thus the dynamic curves are different for different vapours. A waveguide array, in particular an array of waveguides having different pore sizes and/or surface treatment or surface functionalization facilitates discrimination between target analytes. These techniques facilitate discrimination based on analyte size and/or mass and/or boiling point, and discrimination between structural isomers and functional groups.

[0062] Initial feasibility tests were successfully conducted using the structure of FIG. 4, which illustrates a fabricated waveguide. To make the waveguide samples were prepared using p-type doped silicon substrates with a resistivity of 0.01 -cm and (100) crystal orientation. The grooves (1.2 m high and 70 m wide) for the waveguides were etched using standard lithographic process, and then the pores were etched electrochemically using an electrolyte solution containing 30% HF (48% aqueous) and 70% ethanol. The thickness of the core and cladding layers were 3 m each. As the refractive index is determined by the applied current density, it is possible to design multi-layered structures and graded-index planar waveguides in which the porosity is varied through the vertical cross section of the structure.

[0063] The structure of an example resulting waveguide is shown in FIG. 4a, with the top view and the cross-section morphology of porous silicon. The upper core is a low porosity layer and the cladding is a high porosity layer. Alternatively a waveguide may be fabricated with an extended low porosity layer with a raised optical (core) region in which light propagates, as illustrated in FIG. 4b.

[0064] FIGS. 5a and 5b show a vertical cross-section (end-on) view of a waveguide array fabricated in this manner. FIG. 5a shows light coming out of a second waveguide of the array; FIG. 5b shows light coming out of a third waveguide of the array. FIG. 5c shows a view from above of light propagation in one of the waveguides of the array.

[0065] FIG. 6 shows an experimental setup used to carry out near-IR measurements with the porous silicon waveguide. A small tube carrying saturated vapours of different solvents (pentane, methanol, acetone and isopropyl alcohol) was placed above the waveguide in open air. The optical spectrum was then immediately recorded. The measured spectra are shown in FIG. 7.

[0066] As-prepared porous silicon is optically transparent from about 1 m. To facilitate use in the visible, thermal oxidation can be employed to transforms silicon to silica, resulting in porous silica waveguides which are transparent at visible wavelengths and up to about 2.5 m.

[0067] In embodiments of the invention the pore size of the waveguide core typically has a size in the range 5 nm-30 nm. More generally, however, the pore size may lie in a range of from <1 nm up to a size that is smaller than the wavelength used, for example up to 500 nm.

[0068] The pore size is preferably measured at the surface of the core of the waveguide for example by AFM (atomic force microscopy) or SEM. This is because in embodiments the pores do not have a well-defined size within the bulk of the material (FIG. 4).

[0069] The pore size of an individual pore may thus be taken as the average diameter of a pore or the average width of the pore (for non-spherical pores). The pore size of all pores will have a range or distribution of values; the pore size may be taken as the peak of this distribution (the mode of the pore size).

[0070] Other methods to measure porosity and pore size distribution include gas adsorption isotherms of N2 or CO2 at low temperatures. From those, the porosity and pore sizes may be calculated (indirectly), with some assumptions. For example, a pore size distribution may be calculated using the BJH (Barrett-Joyner-Halenda) method, which determines a pore size distribution based on a model of the adsorbent as a collection of cylindrical pores (Barrett, E. P.; L. G. Joyner, P. P. Halenda (1951), The Determination of Pore Volume and Area Distributions in Porous SubstancesComputations from Nitrogen Isotherms. J. Am. Chem. Soc. 73 (1): 373-380).

[0071] Alternatively, however, the porosity (proportion of total pore volume in a material) may be used as a proxy for pore size. This may be determined using a gravimetric method, as described in Porous Silicon in Practice, Prof. Michael J Sailor, Wiley-VCH, 2012, at page 134. Broadly speaking, this etches away a known volume of silicon, weighs the silicon before and after, and calculates the actual volume of silicon removed from the density of silicon, giving the percentage porosity. In some preferred embodiments of the techniques we describe the porosity of the waveguide core may be in the range 10% to 90% porosity, preferably the waveguide core has >40% porosity (the cladding, where present has a higher porosity than the core).

[0072] FIG. 8a shows an analyte sensing system 800 according to an embodiment of the invention. The system comprises an optical source 1 such as an LED, laser or lamp coupled to an optical fibre 2 and optional filter 2a to provide an optical input to a porous waveguide sensor 3 as previously described. Light output from porous waveguide 3 optionally passes through a second filter 4a into a second optical fibre 4 to an optical detector 5 such as a photodetector or spectrometer. The signal output from detector 5 provides an input to a signal processor 6 such as a suitably programmed general purpose computer or dedicated signal processing circuitry.

[0073] Referring now to FIG. 8b, this shows a second analyte sensing system 810, similar to that of FIG. 8a, in which optical fibres 2 and 4 are omitted. The optional filter 2a and optional filter 4a may comprise a narrow passband optical filter such as an interference filter or a multiple passband filter, for example aligned to one or more absorption peaks of a target analyte.

[0074] FIG. 8c shows a waveguide array analyte sensor 830 in which like elements to those previously described are indicated by like reference numerals. Thus the sensor system 830 comprises an optical spatial multiplexer 7 to distribute light from source 1 into each of a plurality of waveguides 3 arranged as a waveguide array 832. In FIG. 8c the outputs of waveguides 3 are combined, optionally by an optical combiner (not shown). As illustrated in the variant waveguide array sensor system 840 of FIG. 8d, one or both of the optical source(s) one and optical detector(s) 5 may be replaced by a plurality of sources/detectors rather than multiplexed.

[0075] FIG. 9 illustrates a temperature controlled porous waveguide 900 for use in any of the sensor systems of FIG. 8. The porous waveguide 900 comprises a substrate 32 bearing a porous waveguide 31, for example comprising a core layer over a lower refractive index cladding layer. A heating element 33 is provided under substrate 32, for example comprising a heating element and/or Peltier effect device, preferably with a temperature sensor for closed loop temperature control of substrate 32 and waveguide 31.

[0076] FIG. 10 shows an embodiment of a sensor probe 10 comprising a porous waveguide sensor 3 as previously described. Fibre optic connections 2, 4 provide respective input and output connections for the porous waveguide sensor, and these are respectively coupled to a remote optical source 1 and to a remote optical detector 5.

[0077] Thus, broadly speaking we have described a sensor device for measuring molecular absorption or Raman spectroscopy of analytes, the device comprising a light source, a porous waveguide and a detector. Here molecular spectroscopy refers particularly to UV, near-IR, mid-IR and far-IR absorption bands of analytes that enter into (are absorbed by) the material of which the porous waveguide is fabricated.

[0078] In embodiments the porous waveguide may be made of silicon, silica, alumina, chalcogenide, titanium dioxide or other materials that are transparent or partially transparent in the desired optical range. Typical dimensions for the porous waveguide have width and height on a micrometre scale, a length in millimetres, and the inner, pore features smaller than the wavelength of light.

[0079] The light source used may provide one or more wavelengths in the range of UV, near-IR, mid-IR and far-IR. The source may be either a broad light source or provide a one or more specific wavelengths. The detector may be, for example, a spectrometer or a photodetector with or without an optical filter. Where used, a filter can be put at the light source and/or at the detector.

[0080] Optionally the external and/or internal surface of the porous waveguide (i.e. the outer face and/or the surface within the pores) may be chemically treated to be either hydrophilic, hydrophobic or functionalised with various chemical groups. Thus the surface may be functionalised with receptor molecules such as DNA, protein, or antibody molecules.

[0081] Embodiments of the sensor may be used to sense an analyte in either the gas or liquid phase.

[0082] In the gas phase, different vapour-phase materials, for example VOCs (volatile organic compounds), will condense differently inside the pores (according to the Kelvin equation). The adsorption and desorption of the gases into and from the pores, may be monitored in real-time to provide time-dependent information.

[0083] The diffusion of analyte into the core, retention of analyte within the core, and analyte condensation is temperature-sensitive. Therefore embodiments comprise a heater plate attached, for example, to the back side of the waveguide. By controlling the temperature one can tune which analytes, for example VOCs, will condense inside the pores, and which will not (or at least which preferentially condense/do not condense). Optionally temperature modulation may be employed in order to obtain a time-dependent temperature data for further discrimination.

[0084] Embodiments of the sensor system may in particular be used to measure gaseous analytes emitted from breath, urine or other biological/human solids, bodily fluids or vapours.

[0085] The sensor or a similar device may also be used to hold analyte as a condensed vapourby allowing the analyte to enter the core and then lowering the temperature to condense the analyte. The stored gas (vapour) may be released by increasing the temperature.

[0086] In addition to the light transmission through the waveguide, embodiments of the sensor system also (optionally simultaneously) monitor the change in the refractive index of the waveguide, for example via white light reflection.

[0087] Additionally or alternatively one or more dc or ac electrical properties of the sensor (waveguide) core may also be monitored, for example resistivity/conductance, and/or impedance. For example a pair of electrodes may be located on (along) the low porosity or core part of the waveguide and connected to an electrical characterisation device/system, for example for measuring resistance, impedance and/or dielectric constant. In a device of the type shown in FIG. 4b the electrodes may be located alongside the core on the low porosity material, for ease of fabrication.

[0088] Such refractive index/electrical data may be used for sensing/discriminating between analytes.

[0089] Optionally the device may have one or more waveguides in parallel. In embodiments each waveguide has different surface chemistry, porosity, temperature, and/or other features. This arrangement can improve the sensitivity/selectivity of the sensor system, by combining the data from the sensor array.

[0090] Embodiments of the waveguide may be fabricated in many ways. For example porous silicon may be produced by electrochemical etching. Optionally a porous silicon waveguide layer (core and optional cladding layer) may be removed from the substrate after fabrication and placed on a substrate other than silicon.

[0091] In another method nanoparticles are deposited on a substrate to create a porous layer. In addition there are many methods which may be employed to exist to produce a porous material using a sol-gel process. Optionally one or more sensitising/functionalising molecules/nanoparticles may be incorporated inside the porous media. These may be employed to tune the optical and/or chemical properties for sensing. Optionally a gain material (a material for providing optical gain) may be incorporated within the waveguide, either distributed within the waveguide or in one or more discrete regions.

[0092] No doubt many other effective alternatives will occur to the skilled person. It will be understood that the invention is not limited to the described embodiments and encompasses modifications apparent to those skilled in the art lying within the spirit and scope of the claims appended hereto.