METHOD FOR INDUSTRIALLY PRODUCING MOGROSIDE, FRUCTUS MOMORDICAE SUGAR/WINE AND MANNITOL FROM FRESH FRUCTUS MOMORDICAE

Abstract

A method for industrially producing mogroside, fructus momordicae sugar/wine and mannitol from fresh fructus momordicae. The method includes the following steps: freshness-preserving and post-ripening: intervally spraying a mixed aqueous solution of -polylysine hydrochloride and calcium propionate, while intervally activating an ozone cycle; and spraying an aqueous solution of ethephon during post-ripening; preparation of mogroside: obtaining mogroside by subjecting the fresh-preserved and post-ripened fructus momordicae to water extraction, microfiltration, gradient processing with combined resins, concentration and spray-drying; preparation of fructus momordicae sugar: obtaining fructus momordicae sugar by compounding the mogroside and a non-sugar sweet substance; preparation of fructus momordicae wine: obtaining fructus momordicae wine by subjecting a macroporous adsorption resin effluent to fermentation and distillation to obtain a distillate, and then formulating same with the mogroside; and preparation of mannitol: obtaining mannitol by subjecting a distillation bottom liquor during the distillation of the fructus momordicae wine to microfiltration, nanofiltration, concentration and crystallization.

Claims

1. A method for industrially producing mogroside, fructus momordicae sugar/wine and mannitol from fresh fructus momordicae comprising the following steps: S1, freshness-preserving and post-ripening: (S1-1) fruit sorting and storing: sorting out and removing old and green fruits and poor-quality fruits from harvested fresh fructus momordicae, and storing the sorted fructus momordicae in a warehouse; (S1-2) freshness-preserving: standing the fructus momordicae in the warehouse for 60-240 days and intervally spraying a mixed aqueous solution of -polylysine hydrochloride and calcium propionate, while intervally activating an ozone cycle, with storage conditions for freshness-preserving being a temperature of 2-6 C., and a daily relative humidity of 80-90% but 55-65% maintained within 12 hours after each spraying of a chemical agent; and (S1-3) saccharifying and post-ripening: raising a temperature to 22-26 C. at a heating rate of 0.15-0.35 C./h in the warehouse, maintaining the relative humidity of 80-90% during the heating process, and a relative humidity of 70-80% when reaching the target temperature, changing air every 22-26 hours to maintain normal oxygen concentration, absorbing carbon dioxide to adjust the concentration of carbon dioxide with an alkaline solution, spraying an aqueous solution of ethephon evenly every 4-6 days, and keeping a post-ripening process for 15-30 days; S2, preparation of mogroside: (S2-1) water extracting: crushing the saccharified and post-ripened fresh fructus momordicae and extracting same with water to obtain an extract; (S2-2) subjecting the extract to ceramic membrane microfiltration; (S2-3) gradient processing with combined resins: subjecting a ceramic membrane clear solution to adsorption and desorption of the following resins sequentially: a first macroporous adsorption resin column, an anion exchange resin, and a second macroporous adsorption resin; and then performing desorption to obtain a combined resin refined solution; (S2-4) concentrating the combined resin refined solution to a concentration of 17-25 Baume degree to obtain a concentrated solution; and (S2-5) spray-drying the concentrated solution to obtain mogroside; S3. preparation of fructus momordicae sugar: (S3-1) taking an appropriate amount of the mogroside from step (S2) and dissolving same by adding purified water as an adhesive; and (S3-2) charging any one or a composition at any ratio of four non-sugar sweet substances of erythritol, arabinose, allulose, and mannitol into a boiling granulator step by step, spraying the adhesive evenly for boiling granulation, and drying same to obtain the fructus momordicae sugar; S4. preparation of fructus momordicae wine: (S4-1) liquid fermentation of first macroporous adsorption resin effluent: collecting a first macroporous adsorption resin feed effluent in step (S2-3), combining same with a water-washing solution, and concentrating same to 5-25 Brix, adding a complex enzyme preparation, and fermenting same until a strong wine aroma appears to obtain a fermentation liquor; (S4-2) distilling the fermentation liquor, collecting a distillation bottom liquor and a distillate separately; and (S4-3) adding the mogroside obtained in step (S2) to the distillate to obtain the fructus momordicae wine; and S5. preparation of mannitol: (S5-1) taking the distillation bottom liquor from step S4, microfiltrating same through a complete set of ceramic membrane equipment, and collecting a filtrate to obtain a ceramic membrane clear solution; (S5-2) subjecting the ceramic membrane clear solution to nanofiltration for decolorization to obtain a discolored solution; and (S5-3) concentrating the discolored solution and adding ethanol while hot, and cooling same for crystallization to obtain mannitol.

2. The method of claim 1, characterized in that, in the freshness-preserving of step (S1-2), the chemical agent is the mixed aqueous solution of -polylysine hydrochloride and calcium propionate, wherein the concentration of the -polylysine hydrochloride is 0.1-0.3%, and the concentration of the calcium propionate is 1.0-2.0%, and the chemical agent is sprayed evenly once every 25-30 days at an amount of 50-100 mL of the mixed aqueous solution of -polylysine hydrochloride and calcium propionate per kg of fructus momordicae, preferably 60-80 mL; and/or the ozone cycle is activated for 40-60 minutes at an amount of ozone of 5-15 ppm every 14-16 days or after a warehouse door is opened and closed.

3. The method of claim 1, characterized in that, in the freshness-preserving of step (S1-2), the storage condition is a temperature of 2-6 C. and a daily relative humidity of 80-90% but 55-65% maintained within 12 hours after each spraying of the chemical agent.

4. The method of claim 1, characterized in that, in the saccharifying and post-ripening of step (S1-3), the alkaline solution is a mixed solution of NaOH and Ca(OH).sub.2, wherein the concentration of NaOH is 0.2-1.0 wt % and the Ca(OH).sub.2 is saturated lime water; and/or the concentration of the ethephon is 400-800 ppm, and 40-80 mL of the aqueous solution of ethephon is sprayed to per kg of fructus momordicae.

5. The method of claim 1, characterized in that, in step (S2-2), the ceramic membrane microfiltration is performed twice to four times, and the temperature is reduced from 45-60 C. to 15-30 C. during the ceramic membrane microfiltration, preferably, the ceramic membrane microfiltration is performed twice, specifically involving cooling the extract to 45-60 C., centrifuging same, and then subjecting same to microfiltration via a first complete set of ceramic membrane equipment to collect a filtrate, further cooling the filtrate to 15-30 C., and then subjecting same to microfiltration via a second complete set of ceramic membrane equipment to collect the filtrate to obtain the ceramic membrane clear solution.

6. The method of claim 1, characterized in that, in step (S2-3), the gradient processing with combined resins is specifically as follows: 1) feeding the ceramic membrane clear solution into the first macroporous adsorption resin column, sequentially loading hot water and a hot alkaline solution at 50-80 C. to the column, then rinsing same with room-temperature water until the distillate has a pH of 6.8-7.5, then desorbing same with hydrous ethanol, collecting a desorbed solution, and concentrating and recovering the ethanol to obtain an adsorption resin-processed solution; 2) adjusting a sugar degree of the adsorption resin-processed solution to 4-8 Brix, feeding same into the basic anion exchange resin column, and feeding 3-5 BV purified water to rinse the resin column after the feeding, and sequentially collecting the effluent and the water-washing solution; and 3) sequentially feeding the anion exchange resin column feed effluent and the water-washing solution into the macroporous adsorption resin column, feeding purified water after the feeding followed by aqueous an solution of citric acid for resin column processing, continuously feeding the purified water until a pH of the resin column effluent is 5.5-7.0, then desorbing with same hydrous ethanol, and collecting a desorbed solution to obtain the combined resin refined solution.

7. The method of claim 6, characterized in that, the first adsorption resin is a macroporous adsorption resin, comprising non-polar D101, LX-100B, LX-T28 and the like, and low-polar AB-8, and is used at an amount of 400-550 kg per 1,000 kg of fresh fructus momordicae; and a processing method comprises after feeding, adding 1.5-3.0 BV of hot purified water at a temperature of 50-80 C., then adding an aqueous solution of NaOH at a temperature of 50-80 C., a concentration of 0.5-1.2% and a volume of 0.6-1.0 BV for resin column processing at a flow rate of 2-3 BV/h, and then adding room-temperature purified water until the pH of the resin column effluent is 6.8-7.5; and/or the anion exchange resin is a basic anion exchange resin comprising gel-type and macroporous acrylic weak-base anion resins, acrylic acid-based strong-base and weak-base anion resins, gel-type and macroporous styrene-based strong-base anion resins, a macroporous acrylic acid strong-base anion resin, and a macroporous styrene-based weak-base anion resin, and is used at an amount of 70-140 kg per 1,000 kg of fresh fructus momordicae; and/or the second adsorption resin is a macroporous adsorption resin, comprising non-polar D101, LX-100B, LX-T28, and low-polar AB-8, and is used at an amount of 140-220 kg per 1,000 kg of fresh fructus momordicae; and a processing method comprises after feeding, adding 1.5-3.0 BV of room-temperature purified water, then adding 0.6-1.0 BV of an aqueous solution of citric acid with a concentration of 0.3-0.6% for resin column processing, and continuously adding purified water until the pH of the resin column effluent is 5.5-7.0.

8. The method of claim 1, characterized in that, in step (S3), the content of mogroside V in the fructus momordicae sugar is 0.10-2.60%, and the sweetness is 1-10 folds of sucrose; and/or in step (S4), the content of mogroside V in the fructus momordicae wine is 0.01-0.04% (w/v).

9. The method of claim 1, characterized in that, in step (S4-1), the sealed fermentation is carried out for 7-30 days; the complex enzyme preparation comprises the following components in parts by mass: 1.5-2.5 parts of sucrase, 1-2 parts of rhizopus, 2-3 parts of protease, 0.5-1 part of esterified red yeasts, 5-10 parts of Saccharomyces cerevisiae, and 1-2 parts of aroma-producing yeasts, and the complex enzyme preparation is used at an amount of 0.4-1.2 kg per 100 kg of the concentrated solution.

10. The method of claim 1, characterized in that, in step (S5-2), the nanofiltration for decolorization is achieved by decolorizing with a complete set of nanofiltration membrane equipment with a molecular weight cut-off of 300-800 Da, and collecting a permeate; in step (S5-3), the discolored solution is concentrated to 15-25 Brix, edible ethanol is added while hot, and the solution is naturally cooled for crystallization, and the crystallization involves adding edible ethanol, stirring same evenly, naturally cooling same at room temperature for crystallization, and filtering same to obtain a crystal I; and after a mother liquor is concentrated, recrystallizing same using the same method to obtain a crystal II, combining the crystal I and the crystal II, and drying same to obtain mannitol.

Description

BRIEF DESCRIPTION OF DRAWINGS

Detailed Description

[0082] The present disclosure is further described in conjunction with examples.

[0083] The fresh fructus momordicae used in the examples of the present disclosure is purchased from Yongfu County, Guilin City, Guangxi, and harvested in late October.

[0084] Unless otherwise specified, -polylysine hydrochloride, calcium propionate, ozone, sodium hydroxide, calcium hydroxide, and ethephon used herein are all commercially available.

[0085] Unless otherwise specified, the used purified water is self-made by a pure water machine, and the used macroporous adsorption resin, anion exchange resin, erythritol, arabinose, allulose and mannitol are all commercially available.

[0086] In the examples of the present disclosure, the content of mogroside V is determined by a high performance liquid chromatography (HPLC) described under fructus momordicae in Chinese Pharmacopoeia (2020); the content of mannitol is determined by a titration method described under mannitol in Chinese Pharmacopoeia (2020); the tastes of mogroside and fructus momordicae sugar are measured by a sensory method; and the pesticide residues in mogroside and the degree of fructus momordicae wine are determined by a gas chromatography (GC).

Example 1

S1 Freshness-Preserving and Post-Ripening

(1) Fruit sorting and storing.

[0087] Old and green fruits and poor-quality fruits were sorted out and removed from the collected fresh fructus momordicae, then the intact, undamaged and mildew-free fructus momordicae was selected, divided into five grades according to the size: extra-large fruit, large fruit, medium fruit, small fruit, and extra-small fruit by using a special fruit measuring board for fructus momordicae, and separated according to the following standards: 240 small fruits, 180 medium fruits, and 140 large fruits/basket; extra-large fruits and extra-small fruits at 13.5 kilograms per basket. The fruits were packed in the baskets, stored and stacked according to the grades, with each stack of lengthwidthheight of 555 baskets, and a stack spacing of 60 cm.

(2) Fresh-Preservation.

[0088] Fresh fructus momordicae stored in a warehouse were treated according to the following procedures and conditions:

Time: Stood for 220 Days.

[0089] (i) Chemical agent: a mixed aqueous solution of 0.25% -polylysine hydrochloride and 1.5% calcium propionate was sprayed evenly once on day 1, 27, 53, 79, 105, 130, 158, 173, and 200, respectively, with the spraying amount of 70 mL per kg of fructus momordicae; [0090] (ii) Gas: an ozone cycle was activated for 45 minutes periodically every 15 days on day 1, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, 180, 195, and 210, on the same day when the chemical agent was sprayed in step (i), and after each irregular inspection, with the concentration of ozone controlled at 123 ppm; Storage conditions: the temperature of a cold storage was set to 3 C. and the daily relative humidity to 85%, which was maintained at 60% within 12 hours after spraying the chemical agent in step (i).

(3) Post-Ripening.

[0091] The fructus momordicae after freshness-preserving and storage were treated according to the following procedures and conditions: [0092] (i) Temperature: the temperature was increased by 1 C. every 4 hours, from 3 C. to 25 C.; [0093] (ii) Relative humidity: during the heating process (0-88 hours), the relative humidity was maintained at 85%, and after the temperature reached 25 C., the relative humidity was 72%; [0094] (iii) Air: air was changed once every 24 hours to maintain the oxygen concentration of approximately 21%; carbon dioxide was absorbed and the concentration of carbon dioxide was adjusted by a mixed solution of 0.5% NaOH and saturated Ca(OH).sub.2 with one mixed solution point set every 4 m.sup.2; and [0095] (iv) Ripening: when the temperature reached 25 C., an aqueous solution of ethephon with the concentration of 600 ppm was sprayed evenly once on day 1, 5, and 10 respectively.

Time: Stood for 15 Days.

[0096] Upon inspection, the surface of more than 65% of the fresh fructus momordicae was turned to a yellow color like mung beans, and about 30% was turned to yellow, with the color coincidence rate90%; characteristic fructus momordicae flavor was obvious, shredded sugar appeared when touched with hands, and the taste was pure; and after 235 days of freshness-preserving (220 days) and post-ripening (15 days), the rate of bad fruits was about 1.3% (based on fresh fructus momordicae in storage).

[0097] In the present disclosure, the conditions for freshness-preserving and post-ripening in step 51 were further changed, while the total time for the freshness-preserving and post-ripening remained unchanged (235 days). The results were shown in Table 1 below.

TABLE-US-00001 TABLE 1 Spraying Days between Concentration Concentration interval of ozone cycles and Rate of -polylysine of calcium mixed aqueous concentration of bad Batch hydrochloride propionate solution of ozone fruits 1 0.25% 1.5% 26 days 15 days, 12 3 ppm 1.3% 2 0.1% 2.0% 26 days 15 days, 12 3 ppm 1.4% 3 0.3% 1.0% 26 days 15 days, 12 3 ppm 1.6% 4 0.25% 1.5% 20 days 15 days, 12 3 ppm 1.3% 5 0.25% 1.5% 30 days 15 days, 12 3 ppm 1.5% 6 0.1% 2.0% 26 days None 2.1% 7 None 1.5% 26 days 15 days, 12 3 ppm 2.2% 8 0.3% None 26 days 15 days, 12 3 ppm 2.4%

[0098] The storage conditions in step (2) during freshness-preserving were changed as follows: the temperature of the cold storage was set to 3 C. and the daily relative humidity to 85 RH %, which was maintained at 60 RH % within 12 hours after spraying the chemical agent in step (i). After testing, there was no obvious change in the freshness-preserving time; but the rate of bad fruits increased from 1.3% to 1.7%.

[0099] Therefore, in summary, the mixed solution sprayed during the freshness-preserving process of the present disclosure contained 0.25% -polylysine hydrochloride and 1.5% calcium propionate, with a spraying interval of 26 days; and the ozone cycle was activated every 15 days to maintain the ozone concentration in the warehouse at a level of 123 ppm.

S2, Preparation of Mogroside:

[0100] (1) Water extraction. 1,500 kg of fresh fructus momordicae were taken after saccharification and post-ripening with the average content of mogroside V at 0.49 wt %. The fructus momordicae was crushed with seeds intact by using a fructus momordicae crusher, and the crushed materials were transported to a 6 m.sup.3 extraction tank with tap water.

[0101] Operating conditions: temperature: 92 C.; time: 2.0, 1.5, and 0.5 hours; frequency: 3 times; water addition amount: 4,000 L, 3,000 L, and 4,000 L; stirring method: compressed air backflushing was started for 5 minutes every 15 minutes. After each extraction, an extract was filtered, the residues from the first and second extractions were extracted for the third time, a third extract was collected separately, and the first and second extracts were combined to obtain 6,200 L of an extract.

[0102] (2) Filtration and clarification. The temperature of the extract was reduced to 55 C. through a plate heat exchanger, firstly, the extract was subjected to horizontal spiral sedimentation and centrifugation, followed by disc centrifugation, and then subjected to microfiltration using a first complete set of ceramic membrane equipment with the material of zirconia and a pore size of 500 nm, and a filtrate was collected. The filtrate was continuously cooled to 24 C. through the plate heat exchanger, and then subjected to microfiltration using a second complete set of ceramic membrane equipment with the material of zirconia and a pore size of 200 nm, and a filtrate was collected to obtain a ceramic membrane clear solution.

[0103] (3) Gradient processing with combined resins.

[0104] A first macroporous adsorption resin. 700 kg of D101 macroporous adsorption resin was pre-installed into a stainless steel chromatography column, and the resin column was subjected to acid-base regeneration for later use. The ceramic membrane clear solution was fed into the macroporous adsorption resin column, after feeding, 1,600 L of hot purified water at a temperature of 70 C. was added, a feed effluent and 1,200 L of a purified water-washing solution were collected to obtain an effluent. Then a NaOH aqueous solution at a temperature of 55 C., a concentration of 0.8%, and a volume of 550 L at a flow rate of 1,400 L/h was used for resin column processing. Then room-temperature purified water was added at a flow rate of 850 L/h until the pH of the resin column effluent was 7.0. Then desorption was performed with 1,800 L of 65% edible ethanol and a desorbed solution was collected. The desorbed solution was concentrated under a reduced pressure at a vacuum of 0.09 MPa and a temperature of 62 C., and ethanol was recovered until there is no alcohol odor to obtain an adsorption resin processed solution.

[0105] Anion exchange resin. 110 kg of D941 weakly basic anion exchange resin was pre-installed into a stainless steel chromatography column internally lined with plastic, and subjected to acid-base regeneration for later use. The sugar degree of an adsorption resin-processed solution was adjusted to 6 Brix, the solution was feed into the basic anion exchange resin column, and after feeding, 500 L of purified water was added to wash the resin column, and a feed effluent and a water-washing solution were collected in sequence.

[0106] A second macroporous adsorption resin. 240 kg of white D101 macroporous adsorption resin with pigments not adsorbed was pre-installed in a stainless steel chromatography column and treated with 90% ethanol and purified water for later use. The anion exchange resin column feed effluent and the water-washing solution were fed into the macroporous adsorption resin column in sequence. After feeding, 500 L of room-temperature purified water was added, 200 L of an aqueous solution of citric acid with the concentration of 0.4% was added for resin column processing, then 600 L of purified water was continuously added until the pH of the resin column effluent was 6.3, then desorption was performed with 520 L of 65% edible ethanol, and a desorbed solution was collected to obtain a combined resin refined solution.

[0107] (4) Concentration. The combined resin refined solution was concentrated under a reduced pressure to 18 Baume degrees at a vacuum of 0.09 MPa and a temperature of 63 C. to obtain a vacuum-concentrated solution.

[0108] (5) Spray-drying. An air inlet temperature of spray-drying was adjusted to 185 C. and an air outlet temperature to 87 C., and the vacuum-concentrated solution was dried to obtain 11.4 kg of mogroside. After testing, the content of mogroside V was 57.11 wt %, the yield of the mogroside V was 88.56%, and a white powder was obtained.

S3. Preparation of Fructus Momordicae Sugar:

[0109] (1) 0.48 kg of the mogroside from step (2) was taken and dissolved with 8 kg of purified water as an adhesive, where the solid content was 5.66%.

[0110] (2) 99.52 kg of erythritol was fed into a fluidized bed at a temperature of 72 C. and a vacuum degree of 0.088 MPa, the adhesive was sprayed evenly at a speed of 3 kg/h, the materials were dried to obtain 99.1 kg of original fructus momordicae sugar. The sweetness of the obtained fructus momordicae sugar was about twice that of sucrose.

S4. Preparation of Fructus Momordicae Wine:

[0111] (1) Liquid fermentation of first adsorption resin effluent. The first adsorption resin feed effluent in step (2) and 1,200 L of a purified water-washing solution were collected, and concentrated to 13 Brix to obtain 825 kg of a concentrated solution. The solution was cooled to room temperature, the ambient temperature was adjusted to 25 C., 5.0 kg of a complex preparation (specific composition: 1.8 parts of sucrase, 1.2 parts of rhizopus, 2.5 parts of protease, 0.6 parts of esterified red yeasts, 9 parts of Saccharomyces cerevisiae, and 1.4 parts of aroma-producing yeasts) was added, and the materials were stirred evenly and subjected to a sealed fermentation for 20 days until a wine aroma was mellow to obtain a fermentation liquor.

[0112] (2) Distillation. The fermentation liquor was distilled and rectified, a distillation bottom liquor and a distillate were respectively collected, and the distillate was fructus momordicae base wine, which had the alcohol content of 53% vol and was 25.3 kg.

[0113] (3) Blending of mogroside. 10 L of the 53% vol distilled fructus momordicae base wine was taken, 0.005 kg of the mogroside in step (2) was added, and the materials were mixed evenly and canned to obtain a fructus momordicae wine.

S5. Preparation of Mannitol:

[0114] (1) Microfiltration of distillation bottom liquor. The distillation bottom liquor was microfiltrated through a complete set of ceramic membrane equipment with the material of zirconia and a pore size of 500 nm, and a filtrate was collected to obtain a ceramic membrane clear solution.

[0115] (2) Nanofiltration for decolorization. The ceramic membrane clear solution was decolorized through a complete set of nanofiltration membrane equipment with a molecular weight cut-off of 500 Da, and a filtrate was collected to obtain a discolored solution.

[0116] (3) Crystallization. The discolored solution was concentrated under a reduced pressure to 22 Brix to obtain 115.2 kg of a concentrated solution. 150 L of 95% edible ethanol was added while hot, the materials were stirred evenly, naturally cool at room temperature to crystallize, and filtered to obtain a crystal I; and a mother liquor was concentrated and recrystallized once using the same method to obtain a crystal II.

[0117] The crystals I and II were combined and dried to obtain 4.3 kg of mannitol.

[0118] After testing, the content of mogroside V in the mogroside was 57.11%, the mogroside was off-white powder, no pesticide residue was detected, and the taste was pure. The sweetness of fructus momordicae sugar was twice that of sucrose and lasted for 6.5 seconds. The alcohol content of the fructus momordicae wine was 53% vol. The content of the mannitol was 99.20%.

Example 2

[0119] Other conditions and operations were the same as those in Example 1, but the difference was that the preparation process of mogroside in step S2 was as follows:

[0120] Steps (1) and (2) are the same as those in Example 1.

[0121] (3) Gradient processing with combined resins.

[0122] The first macroporous adsorption resin. 825 kg of LX-100B macroporous adsorption resin was pre-installed into a stainless steel chromatography column and subjected to acid-base regeneration for later use. A ceramic membrane clear solution was fed into the macroporous adsorption resin column, and after feeding, 1,500 L of hot purified water at a temperature of 80 C. was added, and a feed effluent and 1,200 L of a purified water-washing solution was collected to obtain an effluent. Then a NaOH aqueous solution at a temperature of 50 C., a concentration of 0.9%, and a volume of 800 L was added at a flow rate of 1,500 L/h for resin column processing. Then room-temperature purified water was added at a flow rate of 800 L/h until the pH of the resin column effluent was 7.0. Then desorption was performed with 2,000 L of 60% edible ethanol and a desorbed solution was collected. The desorbed solution was concentrated under a reduced pressure at a vacuum of 0.09 MPa and a temperature of 62 C. and ethanol was recovered until there was no alcohol odor to obtain an adsorption resin-processed solution.

[0123] Anion exchange resin. 120 kg of D900 weakly basic anion exchange resin was pre-installed into a stainless steel chromatography column internally lined with plastic and subjected to acid-base regeneration for later use. The sugar degree of a adsorption resin-processed solution was adjusted to 6 Brix, the solution was feed into the basic anion exchange resin column, and after feeding, 500 L of purified water was added to wash the resin column. A feed effluent and a water-washing solution were collected in sequence.

[0124] A second macroporous adsorption resin. 280 kg of LX-100B macroporous adsorption resin was pre-installed in a stainless steel chromatography column and treated with 90% ethanol and purified water for use. The anion exchange resin column feed effluent and the water washing solution were fed into the macroporous adsorption resin column in sequence. After feeding, 600 L of room-temperature purified water was added, 200 L of an aqueous solution of citric acid with the concentration of 0.4% was added for resin column processing, and then 800 L of purified water was added until the pH of the resin column effluent was 6.4. Then desorption was performed with 550 L of 65% edible ethanol and a desorbed solution was collected to obtain a combined resin refined solution.

[0125] (4) Concentration. The combined resin refined solution was concentrated to 18 Baume degrees at a vacuum of 0.09 MPa and a temperature of 63 C. to obtain a vacuum-concentrated solution.

[0126] (5) Spray-drying. An air inlet temperature of spray-drying was adjusted to 185 C. and an air outlet temperature was 87 C., and the vacuum-concentrated solution was dried to obtain 11.4 kg of mogroside. After testing, the content of mogroside V was 56.68 wt %, the yield of mogroside V was 87.91%, and a white powder was obtained.

Example 3

[0127] Other conditions and operations were the same as those in Example 1, but the difference was the preparation of fructus momordicae sugar in S3:

[0128] (1) 0.48 kg of mogroside was taken from step (2) and dissolved with 8 kg of purified water as an adhesive, where the solid content was 5.66%.

[0129] (2) 50.0 kg of erythritol and 49.0 kg of allulose were fed into a fluidized bed at a temperature of 72 C. and a vacuum degree of 0.088 MPa, the adhesive was sprayed evenly at a speed of 3 kg/h and dried to obtain 98.5 kg of original fructus momordicae sugar. The sweetness of the obtained fructus momordicae sugar is about twice that of sucrose.

Example 4

[0130] Other conditions and operations were the same as those in Example 1, but the difference was the preparation of fructus momordicae wine in S4:

[0131] (1) Liquid fermentation of first adsorption resin effluent. The first adsorption resin feed effluent in step (2) and 1,200 L of a purified water-washing solution were collected, and concentrated to 20 Brix to obtain 550 kg of a concentrated solution. The concentrated solution was cooled to room temperature, the ambient temperature was adjusted to 25 C., 4.5 kg of a complex preparation (specific composition: 1.8 parts of sucrase, 1.2 parts of rhizopus, 2.5 parts of protease, 0.6 parts of esterified red yeasts, 9 parts of Saccharomyces cerevisiae, and 1.4 parts of aroma-producing yeasts) was added, and the materials were stirred evenly and subjected to a sealed fermentation for 30 days until the wine aroma was mellow obtain a fermentation liquor.

[0132] (2) Distillation. The fermentation liquor was distilled and rectified, a distillation bottom liquor and a distillate were collected, and the distillate was fructus momordicae base wine, which had the alcohol content of 26% vol and was 51.2 kg.

[0133] (3) Blending of mogroside. 10 L of 26% vol of the distilled fructus momordicae base wine was taken, 0.030 kg of the mogroside in step (2) was added, and the materials were mixed evenly and canned to obtain a fructus momordicae wine.

Example 5

[0134] Other conditions and operations were the same as those in Example 1, but the difference was that in the filtration for clarification of step S2(2), the temperature of the twice microfiltration through a complete set of ceramic membrane equipment was 55 C. After the obtained clear solution was fed to the chromatography column, due to the presence of some viscous substances, the resin column was hardened to a certain extent, thereby reducing the adsorption of mogroside by the macroporous adsorption resin. The content of mogroside V was 56.24% and the yield was 85.63%.

Comparative Example 1

[0135] Other conditions and operations were the same as in Example 1, except that in the gradient processing with combined resins in step S2(3), the first macroporous adsorption resin was not treated with a hot alkaline solution, but treated conventionally: the ceramic membrane clear solution was fed into the macroporous adsorption resin column, after feeding, room-temperature purified water was added until an effluent was clear, transparent and almost colorless (the water amount was relatively large), then desorption was performed with 65% edible ethanol, a desorbed solution was concentrated under a reduced pressure at a vacuum of 0.09 MPa and a temperature of 62 C., and ethanol was recovered until there was no alcohol odor to obtain an adsorption resin-processed solution. The subsequent steps were the same as those in Example 1. The solution was fed into the anion exchange resin column and the second macroporous adsorption resin column in sequence.

[0136] The content of mogroside V in mogroside in the final product was 51.35% and the yield was 83.51%. There was no significant effect on other products.

Comparative Example 2

[0137] Other conditions and operations were the same as those in Example 1, except that in the gradient processing with combined resins in step S2(3), the processing with the anion exchange resin was omitted. The final mogroside was a light yellow powder. The content of mogroside V was 42.63%, and the yield of mogroside was 88.25%. It indicates that the anion exchange resin was the key to achieve the content of mogroside V in mogroside to be more than 50%. In addition, the taste of the mogroside product obtained without the anion exchange resin operation was not as good as that in Example 1, and was astringent.