Antimicrobial compositions and methods for treating packaged food products

Abstract

The present invention relates to a method of using an antimicrobial composition on a food product where the antimicrobial composition is applied to a food product, the food product is packaged and sealed, and then activation energy is applied to the sealed food product.

Claims

1. A method of reducing microorganisms on a food product comprising: (a) applying an antimicrobial composition to a food product; (b) packaging the food product in a packaging to create a packaged food product; (c) sealing the packaged food product so that additional microorganisms are less likely to enter the packaging; and (d) applying a sub-lethal amount of heat at a temperature of from about 160 F. to about 210 F. to the sealed food product for about 2 to about 20 seconds to activate the antimicrobial composition, wherein the heat is applied in a shrink tunnel, the heat alone does not significantly reduce the microorganism population, and the heat activated antimicrobial reduces the microorganism population by about 1 log to about 3.5 logs.

2. The method of claim 1, wherein the food product is a ready-to-eat meat, or poultry product.

3. The method of claim 1, wherein the packaging is shrink-wrap packaging film and the film does not contain an antimicrobial composition.

4. The method of claim 1, wherein the antimicrobial composition is applied directly to the food product.

5. The method of claim 1, wherein the antimicrobial composition is applied indirectly to the food product.

6. The method of claim 1, wherein the application of the antimicrobial composition to the food product occurs prior to the packaging of the food product.

7. The method of claim 1, wherein the application of the antimicrobial composition to the food product occurs after the packaging of the food product.

8. The method of claim 1, wherein the application of the antimicrobial composition to the food product and the packaging of the food product occur substantially simultaneously.

9. The method of claim 1, wherein the heat is in the form of heated water.

10. The method of claim 1, wherein the antimicrobial composition comprises an active antimicrobial agent selected from the group consisting of fatty acid, acidified sodium chlorite, peroxyacid, and mixtures thereof.

11. The method of claim 10, wherein the active antimicrobial agent is octanoic acid.

12. The method of claim 10, wherein the antimicrobial composition further comprises additional functional ingredients selected from the group consisting of oxidizers, carriers, chelating agents, hydrotropes, thickening agents, gelling agents, foaming agents, film-forming agents, surfactants, coupling agents, acidulants, potentiators, flavoring aids, fragrance, dye, and mixtures thereof.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 illustrates a schematic of an immersion shrink tunnel.

(2) FIG. 2 illustrates a schematic of a cascading shrink tunnel.

(3) FIG. 3 illustrates a schematic of a cascading shrink tunnel with a bottom basin.

(4) FIG. 4 illustrates a schematic of a drip flow shrink tunnel with a bottom jet.

(5) FIG. 5 illustrates a schematic of a drip flow shrink tunnel with a bottom basin.

DETAILED DESCRIPTION OF SOME EMBODIMENTS

(6) The present invention generally provides a method of controlling microorganisms on a food product by applying an antimicrobial composition to the food product or within the final food product package, packaging the food product, sealing the packaging, and, once the food product is sealed, applying activation energy to the sealed food product to further activate the antimicrobial composition inside the packaging. The invention also provides antimicrobial compositions to be used in conjunction with the method.

(7) It is understood that the various embodiments of the present invention described herein may be combined to create a variety of unique embodiments and still remain within the scope of the present invention. Further, it is understood that the examples described herein may be used in conjunction with any of the embodiments described, unless stated otherwise.

DEFINITIONS

(8) For the following defined terms, these definitions shall be applied, unless a different definition is given in the claims or elsewhere in this specification.

(9) All numeric values are herein assumed to be modified by the term about, whether or not explicitly indicated. The term about generally refers to a range of numbers that one of skill in the art would consider equivalent to the recited value (i.e., having the same function or result). In many instances, the term about may include numbers that are rounded to the nearest significant figure.

(10) Weight percent, percent by weight, % by weight, wt %, and the like are synonyms that refer to the concentration of a substance as the weight of that substance divided by the weight of the composition and multiplied by 100.

(11) The recitation of numerical ranges by endpoints includes all numbers subsumed within that range (e.g. 1 to 5 includes 1, 1.5, 2, 2.75, 3, 3.80, 4 and 5).

(12) As used in this specification and the appended claims, the singular forms a, an, and the include plural referents unless the content clearly dictates otherwise. Thus, for example, reference to a composition containing a compound includes a mixture of two or more compounds. As used in this specification and the appended claims, the term or is generally employed in its sense including and/or unless the content clearly dictates otherwise.

(13) The use of the terms antimicrobial in this application does not mean that any resulting products are approved for use as an antimicrobial agent.

(14) In one of several aspects, the present invention provides a method of controlling microorganisms on a food product by applying an antimicrobial composition to the food product or within the final food product packaging, and packaging the food product where the antimicrobial composition is not rinsed off of the food product, and once the packaging is sealed, applying activation energy to the sealed food product to activate the antimicrobial composition inside the packaging.

(15) In certain embodiments, the method can be described in the following steps. First, the unpackaged food product enters the packaging area. Thereafter, an antimicrobial composition is applied in one of several ways to the food product either before, after, or substantially simultaneously with the packaging of the food product or in the final package before or after placing the food product in the final package. The packaging is sealed. Following the packaging and sealing, the sealed food product is exposed to a certain amount of activation energy for a period of time to activate the antimicrobial composition inside the packaging. Each of the steps will now be described in further detail.

(16) Food Product

(17) As used herein, the term food product or food refers to any food or beverage item that may be consumed by humans or mammals. Some non-limiting examples of a food product or food include the following: meat products including ready-to-eat (RTE) meat and poultry products, processed meat and poultry products, cooked meat and poultry products, and raw meat and poultry products including beef, pork, and poultry products; fish products including cooked and raw fish, shrimp, and shellfish; produce including whole or cut fruits and vegetables and cooked or raw fruits and vegetables; pizzas; ready made breads and bread doughs; cheese; eggs and egg-based products, and pre-made food items such as pre-made sandwiches. The present invention is particularly useful for meat and poultry products. Specific examples of meat products including RTE deli or luncheon meats like turkey, ham, and roast beef, hot dogs and sausages. Additionally, the present invention can be used on bacon and pre-made, pre-assembled, or pre-packaged meals such as TV dinners and microwaveable entrees or meals.

(18) Antimicrobial Composition

(19) The present invention includes the application of an antimicrobial composition to the food product. The antimicrobial composition comprises at least one active antimicrobial ingredient. Additionally, the antimicrobial composition may also contain additional functional ingredients that aid in the function of the active antimicrobial ingredient, or impart a desired function or benefit.

(20) There are a variety of active antimicrobial agents that may be used in the present invention. Some non-limiting examples of antimicrobial agents that may be used include fatty acids, C.sub.1-C.sub.12 dicarboxylic acids, percarboxylic acids, halogen compositions or interhalogens thereof, a halogen donor composition, chlorine dioxide, acidified sodium chlorite, ozone, a quaternary ammonium compound, an acid-anionic organic sulfonate or sulfate, a protonated carboxylic acid, or mixtures thereof. Some non-limiting examples of percarboxylic acids include: C.sub.1-C.sub.10 percarboxylic acids, diperoxyglutaric acid, diperoxyadipic acid, diperoxysuccinic acid, diperoxysuberic acid, diperoxymalonic acid, peroxylactic acid, peroxyglycolic acid, peroxyoxalic acid, peroxypyruvic acid, and mixtures thereof. Some non-limiting examples of halogen compounds and interhalogens thereof include: Cl.sub.2, Br.sub.2, I.sub.2, ICl, IBr, ClBr, ICl.sub.2.sup., IBr.sub.2.sup., and mixtures thereof. Non-limiting examples of halogen donor compositions include: HOCl, HOI, HOBr, and the salts thereof, N-iodo, N-bromo, or N-chloro compounds; and N-bromosuccinamide, chloroisocyanuric acid, or 2-N-sodium-N-chloro-p-toluenesulfonamide. A non-limiting example of chlorine dioxide compositions includes chlorine dioxide generated from conventional chemical generators such as those sold by Prominent or preferably generated electrochemically using Halox generators. Some non-limiting examples of acidified sodium chlorite include the composition sold under the tradename SANOVA, and commercially available from Ecolab Inc., (St. Paul, Minn.). A non-limiting example of ozone includes ozone generated electrochemically via high voltage discharge in oxygen. Non-limiting examples of quaternary ammonium compounds include: didecyldimethylammonium chloride, dioctyldimethylammonium chloride, octyldecyldimethylammonium chloride, alkyldimethylbenzylammonium chloride, and mixtures thereof. Non-limiting examples of acid-anionic organic sulfonates and sulfates include: acidic solutions of linear benzylsulfonic acid and sulfonated oleic acid. Non-limiting examples of protonated carboxylic acids include solutions with a pH less than 5 of one or more C.sub.1-C.sub.20 carboxylic acids. See U.S. Pat. Nos. 4,051,058, 4,051,059, 5,200,189, 5,200,198, 5,489,434, 5,718,910, 5,314,687, 5,437,868 for further discussion on peracid chemistry and the formation of an antimicrobial agent formulation. These patents are incorporated herein by reference in their entirety.

(21) The active antimicrobial agent may include one active antimicrobial agent or a combination of more than one active antimicrobial agent. The active antimicrobial agent is preferably a GRAS (generally recognized as safe) or food grade composition. Some non-limiting examples of preferred active antimicrobial agents include fatty acids, acidified sodium chlorite, and peroxyacids such as peroxyacetic acid and peroxyoctanoic acid. The active antimicrobial agent is most preferably octanoic acid.

(22) When applying the antimicrobial composition to the food product, the antimicrobial composition preferably contains from about 0.001 wt. % to about 10 wt. % of the active antimicrobial agent, from about 0.005 wt. % to about 5 wt. % of the active antimicrobial agent, and from about 0.01 wt. % to about 2 wt. % of the active antimicrobial agent. It is understood that different antimicrobial agents have different activities. A person skilled in the art will be able to select the antimicrobial composition and concentration to achieve the desired result.

(23) As previously discussed, the antimicrobial composition may include additional functional ingredients in addition to the active antimicrobial agent. Examples of additional functional ingredients that may be included along with the active antimicrobial agent include oxidizers, carriers, chelating agents, hydrotropes, thickening and/or gelling agents, foaming agents, film-forming agents, surfactants, coupling agents, acidulants, potentiators, flavoring aids, fragrance, dye, and the like. Any additional functional ingredient is preferably a GRAS or food grade ingredient since the antimicrobial composition is preferably applied to the food product. Examples of preferred antimicrobial compositions are described in greater detail in the co-pending patent application entitled, ANTIMICROBIAL COMPOSITIONS FOR USE ON FOOD PRODUCTS, filed concurrently herewith with Ser. No. 11/459,069, the entire disclosure of which is incorporated by reference herein. A person of ordinary skill in the art will be able to formulate compositions depending on the desired active antimicrobial agent, and the desired physical properties so that the various ingredients do not adversely affect each other.

(24) The antimicrobial composition may have a range of physical forms. For example, the antimicrobial composition may be a solid, liquid, structured or thickened liquid or gel, foam, pellet, prill, or a powder. Further, the antimicrobial composition may be a part of a dissolvable film such as polyvinylalcohol (PVA) or cellulose film, or the antimicrobial composition may be blown or extruded with a film, impregnated in a film, or coated on a film. Further, the antimicrobial composition may be formulated as a concentrate composition or a ready-to-use composition. A concentrate composition is often less expensive to ship than a ready-to-use composition. The concentrate refers to the composition that is diluted to form the ready-use-composition. The ready-to-use composition refers to the composition that is applied to the food product.

(25) In certain embodiments, it may be desirable for the active antimicrobial agent to have a lasting effect once the food product is packaged and continue to provide a suppression of growth. For example, it may be desirable under Alternative 1 for the antimicrobial composition to continue to provide an antimicrobial effect over the entire shelf life of the food product and prevent the growth of microorganisms. In other embodiments, it may be desirable for the active antimicrobial agent to cease having an antimicrobial effect shortly after the activation energy is applied.

(26) Application of the Antimicrobial Composition

(27) The antimicrobial composition may be applied to the food product prior to, after, or substantially simultaneously with the packaging of the food product.

(28) The antimicrobial composition may be applied to the food product in several ways. In some embodiments, the antimicrobial composition may be applied directly to the food product in a number of ways including spraying, misting, rolling, and foaming the antimicrobial composition directly onto the food product and the like, and immersing the food product in the antimicrobial composition. The antimicrobial composition may be applied in an injection such as in an injection solution, or the antimicrobial composition may be applied as part of a marinade or tenderizer that is applied to the food product.

(29) In some embodiments, the antimicrobial composition may be indirectly applied to the food product. The antimicrobial composition may be applied to the packaging prior to inserting the food product into the packaging or prior to applying the packaging to the food product. The antimicrobial composition then contacts the food product when the food product is packaged. As used herein, a packaged food product means a food product that has been placed in packaging but not yet sealed. The antimicrobial composition may be applied to the packaging after the food product has been inserted into the packaging or after applying the packaging to the food product (e.g., the antimicrobial composition may be squirted or otherwise introduced into the packaging after the food has been placed in the packaging but prior to sealing the packaging). The antimicrobial composition may be applied to the food product substantially simultaneously with the packaging of the food product. Additionally, it has already been discussed that food packaging or food casing (e.g., hot dog or sausage casing) may be coated, treated, or impregnated with the antimicrobial composition, and the antimicrobial composition is applied to the food product when the food product is placed inside the packaging or casing.

(30) When using the food casing to apply the antimicrobial composition, the antimicrobial composition may be applied to the food product, specifically the hot dog or sausage, by coating, treating, or impregnating the casing with the antimicrobial composition prior to stuffing the casing with the meat product and prior to cooking. While not wanting to be bound to any scientific theory, it is believed that the moisture content of the food product will release the antimicrobial composition from the casing and allow it to coat the surface of the food product. Once the food product is cooked and the casing is removed, the antimicrobial composition is left on the surface of the food product to provide an antimicrobial barrier. The food product is then packaged and the antimicrobial composition is then activated using activation energy.

(31) Packaging

(32) The present invention relates specifically to packaged food products where the packaging is sealed and activation energy is applied to the sealed food product. As previously discussed, a packaged food product refers to a food product that has been placed inside packaging but not yet sealed. The above described food products may be packaged in a variety of ways including vacuum packaging, shrink wrapping, and modified atmosphere packaging. Further, the food products may be packaged in a variety of packaging materials including bags, pouches, films such as shrink films and non-shrink films, trays, bowls, clam shell packaging, web packaging, and hot dog/frankfurter packaging. The present invention is especially useful in conjunction with the shrink wrap packaging that is used in a shrink wrap process.

(33) As discussed above, the packaging of the food product may occur prior to, after, or substantially simultaneously with the application of the antimicrobial composition. However, in the cases where the antimicrobial composition is applied first, and the packaging takes place in a separate step, the packaging step preferably takes place no more than 30 minutes after the application of the antimicrobial composition, no more than 10 minutes after the application of the antimicrobial composition, no more than 60 seconds after the application of the antimicrobial composition, and no more than 5 seconds after the application of the antimicrobial composition. By reducing the amount of time in between the application of the antimicrobial composition to the food product, and when the food product is placed inside the packaging, the likelihood that the food product will be re-contaminated in between the two steps is reduced.

(34) Activation Energies

(35) The method of the present invention includes the application of activation energy to a product to activate the antimicrobial composition. When using activation energy, enough energy must be applied to the antimicrobial composition for a sufficient period of time in order to activate it. The exact amount of energy and length of time may vary depending on the antimicrobial composition, the food product, and the method of energy application. A person skilled in the art will be able to select the desired activation energy, and duration depending on the antimicrobial composition and food product.

(36) Non-limiting examples of suitable activation energies that may be used with all of the methods described herein include heat, pressure, ultraviolet light, infrared light, ultrasonic, radio frequency, microwave radiation, gamma radiation, and the like. Preferred activation energies include heat, pressure, and microwave radiation. It is understood that different activation energies will have different parameters (i.e. amount, duration). A person skilled in the art will be able to select the activation energy and parameters to achieve he desired result.

(37) When heat is used as the activation energy, the heat may be applied in several ways including but not limited to hot water, steam, and hot air.

(38) When using heat as the activation energy, the temperature of the heat is preferably from about 160 F. (71 C.) to about 210 F. (99 C.), from about 180 F. (82 C.) to about 200 F. (93 C.), and from about 190 F. (88 C.) to about 200 F. (93 C.). It is understood that the temperatures provided herein describe the temperature of the composition (e.g., the temperature of the water or air) being applied to the packaged food product, and not the temperature of the food product. For other activation energies described herein, the activation energy used should preferably correspond to the energy applied using heat at the above temperatures.

(39) Non-limiting examples of application time for the above described activation energies, that may be used in conjunction with all of the methods described herein, include about less than 60 seconds, from about 1 to about 60 seconds, from about 2 to about 20 seconds, and from about 3 to about 10 seconds.

(40) It is understood that the heat activation of the present method is different from thermal surface treatment of a food product (e.g., hot water or pasteurization). In a thermal surface treatment process, a thermal source, such as hot water or steam, is applied to a food product either directly to the surface of the food product, or indirectly, by applying heat to the packaging surface. Typical thermal surface treatments apply high temperature heat and/or long exposure times in an effort to reduce the presence of microorganisms (e.g., provide a lethal amount of heat to kill microorganisms). Further, thermal surface treatments require large equipment capital investments and take up a lot of space in a processing facility. Finally, thermal surface treatments have negative organoleptic effects on the food product including color and odor changes and cause increase in liquid purge volumes on meat products. The heat activation of the present invention provides little, if any, reduction in the level of microorganisms (e.g., a sub-lethal amount of heat) because the purpose of the addition of heat is to activate the applied antimicrobial composition which in turn reduces the level of microorganisms, not to use the heat itself to reduce the level of microorganisms. Additionally, the heat used in the method of the present invention does not impact organoleptic properties or purge volumes.

(41) While not wanting to be bound by any scientific theory, it is believed that the present invention works in one of two ways. First, energy is known to increase the kinetics of reactions responsible for cell death. Accordingly, the application of energy in the present invention to food products treated with an antimicrobial composition may increase the efficacy of the antimicrobial composition based on this principle. Second, it is known that the phospholipids in the bilayer of bacterial membranes undergo radical changes in physical state over narrow temperature ranges, sometimes referred to as phase transition temperatures or melting temperatures. Similar conformational and/or denaturative changes take place in the intracellular organelles. It is believed that the present invention takes advantage of these phenomenons by exposing microorganisms to energy in order to reach or pass the phase transition temperature and creating a liquid crystal conformation in the bilayer in which the bilayer becomes more permeable to the antimicrobial composition. Further, the targeted organelles within the microorganism also exhibit conformational changes that make them more susceptible to the antimicrobial composition.

(42) In certain embodiments, the method of the present invention may be carried out in a shrink tunnel using heat as the activation energy, and shrink-wrap film as the packaging. In the shrink wrapping process, a food product is vacuum-packaged in a packaging film that is designed to shrink when heated and form a film around the food product. Once vacuum-packaged, the packaged food product travels through a shrink tunnel that applies heat to the packaging to shrink the packaging around the food product. The heat may be applied in several ways including immersion into a heated bath, or through cascading hot water.

(43) When the present invention is used in conjunction with a shrink tunnel, the present invention may use standard shrink tunnel equipment, or modified shrink tunnel equipment. Some non-limiting examples of shrink tunnels are described in FIGS. 1-5. It is understood that the present invention may be used in any shrink tunnel including variations of the shrink tunnels described in FIG. 1-5 and that the shrink tunnels described in FIGS. 1-5 are intended to be exemplary. When referring to the figures, like structures and elements shown throughout are indicated with like reference numerals.

(44) FIG. 1 illustrates a schematic of an immersion shrink tunnel generally (10). The immersion shrink tunnel (10) is full of heated water. In the immersion shrink tunnel (10), the food product (14) enters the immersion shrink tunnel (10) full of heated water on a conveyor (16). In the method of the present invention, as the food product (14) is immersed in the heated water, the heated water shrinks excess packaging film while activating an antimicrobial composition applied to the food product.

(45) FIG. 2 illustrates a schematic of a cascading shrink tunnel generally (20). The cascading shrink tunnel (20) includes a conveyor (16). The cascading shrink tunnel (20) is fitted with multiple upper cascading water streams (22) that spray heated water on the top of the food product (14). From below the conveyor a jet of heated water (24) sprays the bottom of the food product (14). In the method of the present invention, the food product (14) enters the shrink tunnel (20) on the conveyor (16) and the water streams (22) and jet (24) spray heated water on the food product (14) causing the excess packaging film to shrink while activating an antimicrobial composition applied to the food product.

(46) FIG. 3 illustrates a schematic of a cascading shrink tunnel with a bottom basin generally (30). The cascading shrink tunnel (30) includes a conveyor (16), multiple upper cascading water streams (22), and a bottom basin (32). The bottom basin (32) functions to collect heated water from the cascading water streams (22) and ensure that the bottom of the food product (14) is covered by heated water. In the method of the present invention, the food product (14) enters the shrink tunnel (30) on the conveyor (16) and the water streams (22) spray heated water on the food product (14) as the food product (14) travels through the bottom basin (32) that is full of heated water from the cascading water streams (22). The heated water shrinks excess packaging film while activating an antimicrobial composition applied to the food product.

(47) FIG. 4 illustrates a schematic of a drip flow shrink tunnel with a bottom jet generally (40). The drip flow shrink tunnel includes a conveyor (16) and an upper drip pan (42) that is full of heated water. The upper drip pan (42) includes many small holes (44) for allowing the heated water to flow out of the drip pan (42) and onto the food product (14). The advantage of this type of shrink tunnel is the extended exposure time of the food product (14) to heated water in comparison to the cascading shrink tunnel described in FIG. 2. From below, a jet of heated water (24) sprays the food product (14) with heated water. In the method of the present invention, the food product (14) enters the shrink tunnel (40) on a conveyor (16) and is exposed to heated water from the drip pan (42) and from the jet of heated water (24). The heated water shrinks excess packaging film while activating an antimicrobial composition applied to the food product.

(48) FIG. 5 illustrates a schematic of a drip flow shrink tunnel with a bottom basin generally (50). The shrink tunnel (50) includes a conveyor (16), an upper drip pan (42) that has many small holes (44) for allowing the heated water in the drip pan (42) to flow through, and a bottom basin (32) that is full of heated water. This shrink tunnel also has the advantage of an extended exposure time to heated water in comparison to the cascading shrink tunnel described in FIG. 2. In the method of the present invention, the food product (14) enters the shrink tunnel (50) on a conveyor (16). The food product (14) is exposed to heated water from the upper basin (42) through the small holes (44), and from the lower basin (32) that is full of heated water. The heated water shrinks excess packaging film while activating an antimicrobial composition applied to the food product.

(49) For a more complete understanding of the invention, the following examples are given to illustrate some embodiments. These examples and experiments are to be understood as illustrative and not limiting. All parts are by weight, except where it is contrarily indicated.

EXAMPLES

Example 1

(50) The following is an example of an acidified sodium chlorite (ASC) composition used in the method of the present invention where the ASC composition is activated by passage of the food product through a simulated shrink tunnel.

(51) For this example, sodium chlorite was diluted in water to about 500 ppm to about 1,200 ppm. The pH of the sodium chlorite solution was then adjusted using a GRAS acid such as citric acid or sodium bisulfate to about 2.4 to about 2.6.

(52) TABLE-US-00001 TABLE 1 Acidified Sodium Chlorite Composition Level (ppm) Raw Material QS Water 1,200 ppm Sodium Chlorite 6,000 ppm Citric Acid Final Solution pH ~2.5

(53) An equal-part mixture of five strains of L. monocytogenes including ATCC 19112, ATCC 19114, ATCC 19115, ATCC 7644, and NCTC 10890 suspended in phosphate buffered dilution water, was used as the inoculum. 0.1 milliliters of the inoculum was placed onto a RTE turkey breast, spread with a sterile bent glass rod, followed by storage at 5 C. for 10 minutes to allow for bacterial attachment. RTE turkey breasts were then sprayed with the antimicrobial composition described in Table 1 for 15 seconds. In this example, the volume of the antimicrobial composition applied to each RTE turkey breast was about 15 milliliters. The turkey breasts were placed with bags. The bags were immediately vacuum-packaged, and submerged in 200 F. water for 15 seconds to simulate passage through a shrink tunnel. The bags were then submerged in a 2 C. water bath for 1 minute. Two replicates were completed per treatment. The samples were stored at 5 C. for up to 7 days before being analyzed for populations of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. The RTE turkey breasts were tumbled to recover cells. The resulting suspension was plated in Modified Oxford Medium Agar and the plates were incubated at 35 C. for 72 hours prior to enumeration of L. monocytogenes.

(54) TABLE-US-00002 TABLE 2 Efficacy of ASC and Heat on L. monocytogenes on Packaged, RTE Turkey 1 day 7 days Heat Average Average Average Average Treat- Exposure Log.sub.10 Log.sub.10 Log.sub.10 Log.sub.10 ment (sec) CFU/sample Reduction CFU/sample Reduction Water 0 7.61 NA 9.02 NA ASC 0 7.46 0.15 8.24 0.78 15 6.48 1.13 6.82 2.20

(55) Seven days following treatment, ASC resulted in a 0.78 log reduction of L. monocytogenes. However, the activation of ASC reduced L. monocytogenes populations by 2.20 logs within the food product. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Gombas, D. E., et al. (2003). Survey of Listeria monocytogenes in Ready-to-Eat Foods. Journal of Food Protection (66). 559-569. Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination. Activation of ASC with heat led to a reduction in populations of L. monocytogenes which meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1.

Example 2

(56) The following is an example of an octanoic acid composition used in the method of the present invention where the octanoic acid composition is activated by passage of the food product through a simulated shrink tunnel.

(57) For this example, a solution of 1,000 ppm to about 10,000 ppm octanoic acid, from about 1.0% to about 4.0% ethylene oxide/propylene oxide co-polymer (Pluronic F108), and about 2.0 to about to about 6.0% propylene glycol was adjusted to pH 1.0 with any GRAS acid such as phosphoric acid.

(58) TABLE-US-00003 TABLE 3 Octanoic Acid Composition Level (Wt. %) Raw Material 88.15 Water 2.85 Pluronic F108 5.00 Propylene Glycol 3.00 Phosphoric Acid (75%) 1.00 Octanoic Acid Final Solution pH ~1.18

(59) An equal-part mixture of five strains of L. monocytogenes including ATCC 19112, ATCC 19114, ATCC 19115, ATCC 7644, and NCTC 10890 suspended in phosphate buffered dilution water was used as the inoculum. 0.1 milliliters of the inoculum was placed onto each RTE turkey breast, spread with a sterile bent glass rod, followed by storage at 5 C. for 10 minutes to allow for bacterial attachment. RTE turkey breasts were then sprayed with the antimicrobial composition described in Table 3 for 15 seconds. In this example, the volume of the antimicrobial composition applied to each RTE turkey breast was about 15 milliliters. The turkey breasts were placed into bags. The bags were immediately vacuum-packaged, and submerged in 200 F. water for 15 seconds to simulate passage through a shrink tunnel. The bags were then submerged in a 2 C. water bath for 1 minute. Two replicates were completed per treatment. The samples were stored at 5 C. for 24 hours before analyzed for populations of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. The RTE turkey breasts were tumbled to recover cells. The resulting suspension was plated in Modified Oxford Medium Agar and the plates were incubated at 35 C. for 72 hours prior to enumeration of L. monocytogenes.

(60) TABLE-US-00004 TABLE 4 Efficacy of Octanoic Acid and Heat on L. monocytogenes on, RTE Turkey Heat Exposure Average Log.sub.10 Average Treatment (sec) CFU/sample Log.sub.10 Reduction Water 0 7.61 NA 1% Octanoic Acid 0 6.41 1.20 15 5.57 2.04

(61) Following treatment with 1% octanoic acid, a 1.20 log reduction of L. monocytogenes resulted. However, the activation of octanoic acid reduced L. monocytogenes populations by 2.04 logs within the food product. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination. This example shows that octanoic acid meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1.

Example 3

(62) The following is an example of a peroxyacid composition using the method of the present invention where the peroxyacid composition is activated by passage of the food product through a simulated shrink tunnel.

(63) For this example, a solution of about 100 to about 400 ppm peroxyacetic acid, about 10 to about 50 ppm peroxyoctanoic acid, about 75 to about 300 ppm octanoic acid, and about 32 to about 150 ppm hydrogen peroxide was adjusted to about pH 1.5 with phosphoric acid.

(64) TABLE-US-00005 TABLE 5 Peroxyacid Composition Level (ppm) Raw Material 775 Acidic Acid 200 Peroxyacetic Acid 140 Octanoic Acid 75 Hydrogen Peroxide 25 Peroxyoctanoic Acid 10 HEDP Final Solution pH ~1.5

(65) An equal-part mixture of five strains of L. monocytogenes including ATCC 19112, ATCC 19114, ATCC 19115, ATCC 7644, and NCTC 10890 suspended in phosphate buffered dilution water, was used as the inoculum. 0.1 milliliters of the inoculum was placed onto each RTE roast beef sample, spread with a sterile bent glass rod, followed by storage at 5 C. for 10 minutes to allow for bacterial attachment. RTE roast beef samples were then sprayed with the antimicrobial composition described in Table 5 for 15 seconds. In this example, the volume of the antimicrobial composition applied to each RTE roast beef sample was about 15 milliliters. The roast beef samples were placed into bags. The bags were immediately vacuum-packaged, and submerged in 200 F. water for 15 seconds to simulate passage through a shrink tunnel. The bags were then submerged in a 2 C. water bath for 1 minute. Two replicates were completed per treatment. The samples were stored at 5 C. for 24 hours before analyzed for populations of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. The RTE roast beef samples were tumbled to recover cells. The resulting suspension was plated in Modified Oxford Medium Agar and the plates were incubated at 35 C. for 72 hours prior to enumeration of L. monocytogenes.

(66) TABLE-US-00006 TABLE 6 Efficacy of Peroxyacid and Heat on L. monocytogenes on RTE Roast Beef Heat Exposure Average Log.sub.10 Average Treatment (sec) CFU/sample Log.sub.10 Reduction Water 0 8.72 NA Peroxyacid 0 8.13 0.59 Antimicrobial 15 7.74 0.98

(67) Following treatment with peroxyacid, a 0.59 log reduction of L. monocytogenes resulted. However, the activation of peroxyacid reduced L. monocytogenes populations by 0.98 logs within the food product. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination.

Example 4

(68) The following is an example of an ASC composition and an octanoic acid composition together used in the method of the present invention where both compositions are activated by passage of the food product through a simulated shrink tunnel.

(69) For this example sodium chlorite was diluted in water to about 500 ppm to about 1,200 ppm. The pH of the sodium chlorite was then adjusted using any GRAS acid such as citric acid or sodium bisulfate to about 2.4 to about 2.6. The second solution of octanoic acid was prepared containing from about 1,000 ppm to about 10,000 ppm of octanoic acid, from about 1.0 to about 4.0 wt. % ethylene oxide/propylene oxide copolymer (Pluronic F108), and about 2.0 to about 6.0 wt. % propylene glycol. The octanoic acid solution was adjusted to pH 2.0 with any GRAS acid such as phosphoric acid.

(70) TABLE-US-00007 TABLE 7 Acidified Sodium Chlorite Composition Level (ppm) Raw Material QS Water 1200 Sodium Chlorite 6000 Citric Acid Final Solution pH ~2.5

(71) TABLE-US-00008 TABLE 8 Octanoic Acid Composition Level (Wt. %) Raw Material 90.95 Water 2.85 Pluronic F108 5.00 Propylene Glycol 0.20 Phosphoric Acid (75%) 1.00 Octanoic Acid Final Solution pH ~2.0

(72) An equal-part mixture of five strains of L. monocytogenes including ATCC 19112, ATCC 19114, ATCC 19115, ATCC 7644, and NCTC 10890, suspended in phosphate buffered dilution water, was used as the inoculum. 0.1 milliliters of the inoculum was placed onto each RTE turkey breast, spread with a sterile bent glass rod, followed by storage at 5 C. for 10 minutes to allow for bacterial attachment. The ASC solution was spray applied to the surface of the RTE product. Immediately after, the turkey breasts were placed into bags. The octanoic acid solution was then applied to the RTE product in the bag. In this example, the volume of each of the antimicrobial composition applied to each RTE turkey breasts was about 15 milliliters. The bags were immediately vacuum-packaged, and submerged in 200 F. water for 2 or 15 seconds to simulate passage through a shrink tunnel. The bags were then submerged in a 2 C. water bath for 1 minute. Two replicates were completed per treatment. The samples were stored at 5 C. for up to 14 days before analyzed for populations of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. The RTE turkey breasts were tumbled to recover cells. The resulting suspension was plated in Modified Oxford Medium Agar and the plates were incubated at 35 C. for 72 hours prior to enumeration of L. monocytogenes.

(73) TABLE-US-00009 TABLE 9 Efficacy of ASC and Octanoic Acid and Heat on L. monocytogenes on RTE Turkey 1 day of storage Heat Average 14 days of storage Expo- Log.sub.10 Average Average Average sure CFU/ Log.sub.10 Log.sub.10 Log.sub.10 Treatment (sec) sample Reduction CFU/sample Reduction Untreated 2 4.09 NA 5.19 NA ASC 2 2.15 1.94 2.05 3.14 15 <1.70.sup.a >2.39 <1.70 >3.49 ASC & 2 1.94 2.15 <1.70 >3.49 Octanoic Acid 15 <1.70 >2.39 <1.70 >3.49 .sup.aLimit of detection of the assay was 1.70 log.sub.10 CFU/sample

(74) Activation of both ASC and octanoic acid with heat resulted in the absence of recoverable colonies on RTE turkey breasts following 14 days of storage. Thus, once activated, the antimicrobial compositions substantially suppress the growth of L. monocytogenes on treated RTE foods. This example shows that the use of ASC and octanoic acid meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1 and may meet the requirements of an antimicrobial agent or process which suppresses the growth of L. monocytogenes as described in FSIS Form 10,240-1.

Example 5

(75) The following example determined the efficacy of an octanoic acid solution at killing Listeria monocytogenes on turkey frankfurters when used in the method of the present invention where the octanoic acid composition was activated by simulating passage of the food product through a simulated shrink tunnel.

(76) For this example, an aqueous solution of 930 ppm octanoic acid prepared with the following composition: 930 ppm octanoic acid, 830 ppm 1-hydroxyethylidene-1,1-diphosphonic acid (Dequest 2010), 1,250 ppm 1-octanesulfonae and acidified to about pH 1.5 using phosphoric acid. An equal-part mixture of five strains of L. monocytogenes including ATCC 19112, ATCC 19114, ATCC 19115, ATCC 7644, and NCTC 10890, suspended in phosphate buffered dilution water, was used as the inoculum. 0.125 milliliters of the inoculum was pipetted onto each turkey frankfurter within a sterile polyethylene bag. The frankfurters were stored at 10 C. for 10 minutes to allow for bacteria attachment. 1 milliliter of the octanoic acid formula (or sterile water for the control) was added to each bag. The bags were vacuum-packaged, and submerged in 200 F. water for up to 15 seconds to simulate passage through a shrink tunnel. The bags were then submerged in a 2 C. water bath for 1 minute. Two replicates were completed per treatment. The samples were stored at 5 C. for about 24 hours before analyzed for populations of L. monocytogenes. Fifteen milliliters of University of Vermont broth were added to each bag. The frankfurters were massaged for 1 minute to recover cells. The resulting suspension was plated in Modified Oxford Medium Agar and the plates were incubated at 35 C. for 72 hours prior to enumeration of L. monocytogenes.

(77) TABLE-US-00010 TABLE 10 Efficacy of 930 ppm Octanoic Acid in Killing L. monocytogenes on Turkey Frankfurters Average Log.sub.10 Treatment Heat Exposure Average Log.sub.10 Reduction Vs. Solution (sec) CFU/sample Control Water (control) None 7.20 Not Applicable 940 ppm None 6.81 0.39 Octanoic Acid 5 5.40 1.80 15 5.32 1.88

(78) The treatment of turkey frankfurters with 930 ppm octanoic acid without heat resulted in a 0.39 log reduction of L. monocytogenes. However, the activation of octanoic acid with heat led to up to a 1.88 log reduction. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination. This example shows that octanoic acid meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1.

Example 6

(79) The following example determined the efficacy of 1.0% octanoic acid solution at reducing L. monocytogenes on RTE oven roasted turkey breasts where the octanoic acid was activated by simulating passage of the food product through a simulated immersion shrink tunnel. For this example an aqueous solution of 1% octanoic acid comprised of 1% octanoic acid and 5% Polysorbate 80 as a coupler then acidified to pH 2.0 using 0.3% phosphoric acid. An equal-part mixture of five strains of L. monocytogenes, including ATCC 19112, ATCC 19114, ATCC 19115, ATCC 7644, and NCTC 10890, suspended in a phosphate buffered dilution water, was used as the inoculum. Sample surfaces were spot-inoculated with 50 microliters of the inoculum. The inoculum was spread using a sterile bent glass rod. Inoculated samples were stored at 5 C. for 30 minutes before treatment to allow for bacterial attachment. The inoculated turkey samples were transferred to shrink bags. Fifteen milliliters of the octanoic acid formula were added to bags which were immediately vacuum-packaged and submerged in water heated to 190 F. for 10 seconds (treated samples) or 2 seconds (untreated control samples) prior to being placed into a 2 C. water bath for 1 minute. Five replicates were completed per treatment. The samples were stored at 5 C. for 24 hours before analyzed for populations of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. The turkey samples were tumbled for 50 rotations and the resulting suspension was plated in Modified Oxford Medium Agar. Plates were incubated at 35 C. for 48 hours before the pathogen was enumerated.

(80) TABLE-US-00011 TABLE 11 Efficacy of 1.0% Octanoic Acid in L. monocytogenes on RTE Oven Roasted Turkey Breasts Average Log.sub.10 Log.sub.10 Reduction Treatment Solution CFU/sample Vs. Control Untreated Control 4.91 Not Applicable 1.0% Octanoic Acid 2.49 2.42

(81) The treatment of the oven roasted turkey breasts with 1.0% octanoic acid resulted in a 2.42 log reduction of L. monocytogenes. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination. This example shows that octanoic acid meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1.

Example 7

(82) The following example determined the efficacy of 1.0% solution of octanoic acid against L. monocytogenes on turkey ham, where the octanoic acid composition was activated by simulating passage of the food product through a simulated shrink tunnel. For this example the same aqueous solution used in Example 6 was used. An equal-part mixture of five strains of L. monocytogenes including ATCC 19112, ATCC 19114, ATCC 19115, ATCC 7644 and NCTC 10890, suspended in phosphate buffered dilution, water, was used as the inoculum. Sample surfaces were spot-inoculated with 50 microliters of the inoculum. The inoculum was spread using a sterile bent glass rod. Inoculated samples were stored at 5 C. for 30 minutes before treatment to allow for bacterial attachment. Inoculated turkey ham samples were transferred to shrink bags. Ten milliliters of the octanoic acid composition were added to the bags which were immediately vacuum-packaged and submerged in water heated to a 190 F. for 10 seconds (treated samples) or 2 seconds (untreated control samples) prior to being placed into a 2 C. water bath for 1 minute. Five replicates were completed per treatment. The samples were stored at 5 C. for 24 hours before analyzed for populations of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. Turkey ham samples were tumbled for 50 rotations and the resulting suspension was plated in Modified Oxford Medium Agar. Plates were incubated at 35 C. for 48 hours before the pathogen was enumerated.

(83) TABLE-US-00012 TABLE 12 Efficacy of 1.0% Octanoic Acid in Killing L. monocytogenes on Turkey Ham Average Log.sub.10 Log.sub.10 Reduction Treatment Solution CFU/sample Vs. Control Untreated Control 4.86 Not Applicable 1.0% Octanoic Acid 2.52 2.34

(84) The treatment of turkey ham with 1.0% octanoic acid resulted in a 2.34 log reduction of L. monocytogenes. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination. This example shows that octanoic acid meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1.

Example 8

(85) The following example determined the efficacy of a 1.0% octanoic acid solution against L. monocytogenes on roast beef where the octanoic acid composition was activated by passage of the food product through a simulated shrink tunnel. For this example an aqueous solution of 1% octanoic acid using 2.85% ethylene oxide/propylene oxide co-polymer (Pluronic F108) as a coupler, was prepared and acidified pH 2.0 using 0.3% phosphoric acid. An equal-part mixture of five strains of L. monocytogenes including ATCC 19112, ATCC 19114, ATCC 19115, ATCC 7644, and NCTC 10890, suspended in phosphate buffered dilution, water was used as the inoculum. Roast beef surfaces were spot-inoculated with 50 microliters of the inoculum. The inoculum was spread using a sterile bent glass rod. Inoculated roast beef samples were stored at 5 C. for 30 minutes before treatment to allow for bacterial attachment. The roast beef samples were treated with octanoic acid via a spray which resulted in retention of approximately 15 milliliters of the octanoic acid formula to each treated sample. Roast beef samples were placed in shrink bags which were immediately vacuum-packaged and submerged in water heated to 200 F. for 2, 6, or 10 seconds (treated samples) or 2 seconds (untreated control samples) prior to being placed into a 2 C. water bath for 1 minute. Ten replicates were completed per treatment. Samples were stored at 5 C. for 24 hours before being analyzed for population of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. Roast beef samples were tumbled for 50 rotations and the resulting suspension was plated in Modified Oxford Medium Agar. Plates were incubated at 35 C. for 48 hours before the pathogen was enumerated.

(86) TABLE-US-00013 TABLE 13 Efficacy of 1.0% Octanoic Acid in Killing L. monocytogenes on Roast Beef Heat Average Log.sub.10 Exposure Log.sub.10 Reduction Treatment Solution (seconds) CFU/sample Vs. Control Untreated Control 2 4.76 Not Applicable 1.0% Octanoic Acid 2 3.02 1.74 6 2.73 2.03 10 2.51 2.26

(87) The results of the study clearly demonstrate the increase in efficacy following activation of octanoic acid within the food product. Activation of 1% octanoic acid with heat reduced populations of the pathogen by up to 2.26 logs. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination. This example shows that octanoic acid meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1.

Example 9

(88) The following example determined the efficacy of a 1.0% octanoic acid solution against L. monocytogenes on turkey breasts and roast beef where the octanoic acid composition was activated by passage through a conventional cascading shrink tunnel.

(89) For this example an aqueous solution of 1% octanoic acid using 3% Polysorbate 20 as a coupler, was prepared and acidified pH 2.0 using 0.3% phosphoric acid. An equal-part mixture of five strains of L. monocytogenes, including Scott A (serotype 4b, human isolate), H7750 (not serotyped, frankfurter isolate), AC33 (not serotyped, cooked ham isolate), LM108M (serotype 1/2b, salami isolate), and F6854 (serotype 1/2a, frankfurter isolate), suspended in phosphate buffered dilution water were used. Turkey breast and roast beef surfaces were spot-inoculated with 50 microliters of the inoculum. The inoculum was spread using a sterile bent glass rod. Inoculated RTE products were stored at 5 C. for 30 minutes before treatment to allow for bacterial attachment. RTE samples were placed in shrink bags. The RTE samples were treated with octanoic acid via a direct application of about 15 milliliters of the octanoic acid formula to each treated sample. The bags were immediately vacuum-packaged and sent through a Cryovac ST-101 cascading shrink tunnel. The shrink tunnel conveyor belt speed was set to expose the RTE foods to 200 F. water for about 5 seconds. Three replicates were completed per treatment. Samples were stored at 5 C. for 24 hours before being analyzed for population of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. RTE food product samples were tumbled for 50 rotations and the resulting suspension was plated in Modified Oxford Medium Agar. Plates were incubated at 35 C. for 48 hours before the pathogen was enumerated.

(90) TABLE-US-00014 TABLE 14 Efficacy of 1% Octanoic Acid and Heat in Killing L. monocytogenes on the Surfaces of Turkey Breasts and Roast Beef Average Log.sub.10 Antimicrobial Log.sub.10 Reduction RTE Food Treatment Heat CFU/sample Vs. Control Turkey None (control) No Heat 5.05 NA Breasts 1% Octanoic No Heat 3.72 1.33 Acid Cascading 3.08 1.97 water, 200 F. for 5 sec Roast None (control) No Heat 4.92 NA Beef 1% Octanoic No Heat 4.11 0.81 Acid Cascading 3.66 1.26 water, 200 F. for 5 sec

(91) The treatment of turkey breasts and roast beef with 1% octanoic acid and heat resulted in log reductions of 1.97 and 1.26, respectively, of L. monocytogenes. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination. This example shows that octanoic acid meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1.

Example 10

(92) The following example determined the efficacy of a 1.0% octanoic acid solution against L. monocytogenes on roast beef where the octanoic acid composition was activated by passage through a modified drip flow shrink tunnel.

(93) For this example, a solution of 1% octanoic acid using 3% Polysorbate 20 as a coupler was prepared and acidified pH 2.0 using 0.3% phosphoric acid. A second solution of 1% octanoic acid using 3% Polysorbate 20 as a coupler was prepared which was acidified to pH 4.0 using 2.55% citric acid and 0.6% sodium hydroxide. The efficacy of both formulas was evaluated. An equal-part mixture of five strains of L. monocytogenes, including Scott A (serotype 4b, human isolate), H7750 (not serotyped, frankfurter isolate), AC33 (not serotyped, cooked ham isolate), LM108M (serotype 1/2b, salami isolate), and F6854 (serotype 1/2a, frankfurter isolate), suspended in phosphate buffered dilution water were used. Roast beef samples were spot-inoculated with 50 microliters of the inoculum. The inoculum was spread using a sterile bent glass rod. Inoculated RTE food product samples were stored at 5 C. for 30 minutes before treatment to allow for bacterial attachment. RTE food product samples were placed in shrink bags. The RTE food product samples were treated with octanoic acid via a direct application of about 15 milliliters of either octanoic acid formula to each treated sample. The bags were immediately vacuum-packaged and sent through a modified Cyrovac ST-101 drip flow shrink tunnel. The shrink tunnel conveyor belt speed was set to expose the RTE foods to 200 F. water for about 7 seconds. Control samples were subjected to a 2-second submersion in water heated to 200 F. Three replicates were completed per treatment. Samples were stored at 5 C. for 24 hours before being analyzed for population of L. monocytogenes. Fifty milliliters of University of Vermont broth were added to each bag. RTE food product samples were tumbled for 50 rotations and the resulting suspension was plated in Modified Oxford Medium Agar. Plates were incubated at 35 C. for 48 hours before the pathogen was enumerated.

(94) TABLE-US-00015 TABLE 15 Efficacy of 1% Octanoic Acid and Heat in Killing L. monocytogenes on Roast Beef Average Log.sub.10 Antimicrobial Log.sub.10 Reduction Treatment Heat CFU/sample Vs. Control None (control) 2 sec 4.31 NA 1% Octanoic Acid 2 sec 3.13 1.18 acidified to pH 2 Drip flow water, 2.26 2.05 with phosphoric 200 F. for 7 sec acid 1% Octanoic Acid 2 sec 2.22 2.09 acidified to pH 4 Drip flow water, 2.05 2.26 with citric acid 200 F. for 7 sec

(95) Treatment of roast beef with 1% octanoic acid acidified to pH 2 with phosphoric acid and heat resulted in a 2.05 log reduction of L. monocytogenes, whereas the antimicrobial composition alone reduced populations of the pathogen by 1.18 logs. Treatment of roast beef with 1% octanoic acid acidified to pH 4 with citric acid and heat resulted in a 2.26 log reduction of L. monocytogenes, whereas the antimicrobial composition alone reduced populations of the pathogen by 2.09 logs. It has been published that naturally occurring L. monocytogenes contamination levels in RTE meat products is generally low (about <1 CFU/g). Thus, once activated, the antimicrobial composition renders the RTE product essentially free of Listeria monocytogenes contamination. This example shows that octanoic acid meets FSIS requirements of a post-lethality treatment as described in FSIS Form 10,240-1.

(96) The foregoing summary, detailed description, and examples provide a sound basis for understanding the invention, and some specific example embodiments of the invention. Since the invention can comprise a variety of embodiments, the above information is not intended to be limiting. The invention resides in the claims.