CHINESE HERBAL MEDICINE COMPOSITION FOR RELIEVING HEARING LOSS AND PREPARATION METHOD AND USE THEREOF

Abstract

The invention discloses a Chinese herbal medicine composition for relieving hearing loss and a preparation method and use thereof. The Chinese herbal medicine composition is prepared from at least two of the medicinal and edible raw materials of rhizoma curcumae longae, Dendrobium nobile and fructus Piperis longi, can effectively reduce the content of NO secreted by cells, reduces cell inflammation, and has a good effect of relieving hearing loss induced by LPS. The Chinese herbal medicine composition is not a simple combination of traditional Chinese medicines and has a synergistic effect. The Chinese herbal medicine composition can significantly reduce ABR value, MDA, and glutamic acid of a patient with hearing loss, significantly relieves hearing loss, and can be developed into functional foods and medicines for relieving hearing loss as an active ingredient.

Claims

1. A product comprising rhizoma curcumae longae, wherein the product has any one of functions from (1) to (6): (1) preventing and/or treating hearing loss; (2) reducing an auditory brainstem response (ABR) value; (3) reducing the content of malondialdehyde; (4) reducing the content of glutamic acid; (5) reducing the content of IL-4; and (6) inhibiting production of inflammatory factors.

2. A Chinese herbal medicine composition prepared from the raw materials comprising any one of (7) to (10): (7) 35-85 parts by weight of rhizoma curcumae longae and 35-85 parts by weight of fructus Piperis longi; (8) 35-85 parts by weight of rhizoma curcumae longae and 25-65 parts by weight of Dendrobium nobile; (9) 35-85 parts by weight of rhizoma curcumae longae, 25-65 parts by weight of Dendrobium nobile and 35-85 parts by weight of fructus Piperis longi; and (10) 25-65 parts by weight of Dendrobium nobile and 35-85 parts by weight of fructus Piperis longi.

3. The Chinese herbal medicine composition according to claim 2, prepared from the raw materials comprising any one of (11) to (15): (11) 35-85 parts by weight of rhizoma curcumae longae, 35-85 parts by weight of fructus Piperis longi, 15-65 parts by weight of ginseng, 15-65 parts by weight of mulberry, 5-45 parts by weight of Radix puerariae, 5-45 parts by weight of Cornus officinalis, 5-45 parts by weight of Semen ziziphi spinosae, 5-45 parts by weight of Radix astragali, 5-45 parts by weight of peach kernel, 3-22 parts by weight of licorice, and 3-22 parts by weight of honeysuckle; (12) 35-85 parts by weight of rhizoma curcumae longae, 25-65 parts by weight of Dendrobium nobile, 15-65 parts by weight of ginseng, 15-65 parts by weight of mulberry, 5-45 parts by weight of Radix puerariae, 5-45 parts by weight of Cornus officinalis, 5-45 parts by weight of Semen ziziphi spinosae, 5-45 parts by weight of Radix astragali, 5-45 parts by weight of peach kernel, 3-22 parts by weight of licorice, and 3-22 parts by weight of honeysuckle; (13) 35-85 parts by weight of rhizoma curcumae longae, 25-65 parts by weight of Dendrobium nobile, 35-85 parts by weight of fructus Piperis longi, 15-65 parts by weight of ginseng, 15-65 parts by weight of mulberry, 5-45 parts by weight of Radix puerariae, 5-45 parts by weight of Cornus officinalis, 5-45 parts by weight of Semen ziziphi spinosae, 5-45 parts by weight of Radix astragali, 5-45 parts by weight of peach kernel, 3-22 parts by weight of licorice, and 3-22 parts by weight of honeysuckle; (14) 25-65 parts by weight of Dendrobium nobile, 35-85 parts by weight of fructus Piperis longi, 15-65 parts by weight of ginseng, 15-65 parts by weight of mulberry, 5-45 parts by weight of Radix puerariae, 5-45 parts by weight of Cornus officinalis, 5-45 parts by weight of Semen ziziphi spinosae, 5-45 parts by weight of Radix astragali, 5-45 parts by weight of peach kernel, 3-22 parts by weight of licorice, and 3-22 parts by weight of honeysuckle; and (15) 35-85 parts by weight of rhizoma curcumae longae, 15-65 parts by weight of ginseng, 15-65 parts by weight of mulberry, 5-45 parts by weight of Radix puerariae, 5-45 parts by weight of Cornus officinalis, 5-45 parts by weight of Semen ziziphi spinosae, 5-45 parts by weight of Radix astragali, 5-45 parts by weight of peach kernel, 3-22 parts by weight of licorice, and 3-22 parts by weight of honeysuckle.

4. A preparation method of the Chinese herbal medicine composition according to claim 2, comprising: conducting water extraction and/or alcohol extraction of any one of (7) to (10) as a raw material to obtain the Chinese herbal medicine composition.

5. A preparation method of the Chinese herbal medicine composition according to claim 3, comprising: conducting water extraction and/or alcohol extraction of any one of (11) to (15) as a raw material to obtain the Chinese herbal medicine composition.

6. A product comprising the Chinese herbal medicine composition according to claim 2, wherein the product has any one of functions from (1) to (6): (1) preventing and/or treating hearing loss; (2) reducing an auditory brainstem response (ABR) value; (3) reducing the content of malondialdehyde; (4) reducing the content of glutamic acid; (5) reducing the content of IL-4; and (6) inhibiting production of inflammatory factors.

7. The product according to claim 6, wherein the type of the product comprises a food and a medicine.

8. A product comprising the Chinese herbal medicine composition according to claim 3, wherein the product has any one of functions from (1) to (6): (1) preventing and/or treating hearing loss; (2) reducing an auditory brainstem response (ABR) value; (3) reducing the content of malondialdehyde; (4) reducing the content of glutamic acid; (5) reducing the content of IL-4; and (6) inhibiting production of inflammatory factors.

9. A product comprising the Chinese herbal medicine composition according to claim 2.

10. The product according to claim 9, wherein the type of the product comprises a food and a medicine.

11. The product according to claim 10, wherein the type of the food may comprise a beverage, a biscuit, a candy and a pastry.

12. The product according to claim 10, wherein a dosage form of the medicine comprises a capsule, a tablet, a granule, a mixture, a decoction, a pill, an electuary, a dripping pill, a liniment, a tincture and a wine.

Description

DETAILED DESCRIPTION

[0179] The invention will be further described in detail below with specific examples.

[0180] It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention.

[0181] The experimental methods not indicated with specific conditions in the following examples are usually conducted conventionally or according to recommendation by a manufacturer. Unless otherwise specified, the materials, reagents, etc. used in the examples are commercially available.

Example 1: Chinese Herbal Medicine Extract for Relieving Hearing Loss

[0182] 1. A Chinese herbal medicine extract for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of rhizoma curcumae longae.

[0183] 2. A preparation method of the Chinese herbal medicine extract for relieving hearing loss included the following steps:

[0184] S1. a dried rhizoma curcumae longae product was crushed and sieved through a 60-mesh sieve, the medicinal fine powder was measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal material was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for late use;

[0185] S2. the medicine residue of step 51 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at a rotating drum speed of 20,000 r/min) and a filtrate was collected for later use;

[0186] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0187] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.5 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine extract with the moisture content of 3.28%.

Example 2: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0188] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of rhizoma curcumae longae and 80 parts of fructus Piperis longi.

[0189] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0190] S1. a dried rhizoma curcumae longae product and a dried fructus Piperis longi product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for late use;

[0191] S2. the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0192] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0193] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.5 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 2.99 w/w %.

Example 3: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0194] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of rhizoma curcumae longae and 60 parts of Dendrobium officinale.

[0195] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0196] S1. a dried rhizoma curcumae longae product and a dried Dendrobium officinale product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for late use;

[0197] S2. the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0198] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0199] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.5 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 3.27 w/w %.

Example 4: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0200] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of fructus Piperis longi and 60 parts of Dendrobium officinale.

[0201] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0202] S1. a dried fructus Piperis longi product and a dried Dendrobium officinale product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for late use;

[0203] S2. the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0204] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0205] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.5 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 3.54 w/w %.

Example 5: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0206] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of rhizoma curcumae longae, 80 parts of fructus Piperis longi and 60 parts of Dendrobium officinale.

[0207] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0208] S1. a dried rhizoma curcumae longae product, a dried fructus Piperis longi product and a dried Dendrobium officinale product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0209] S2. the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0210] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 MPa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0211] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.5 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 4.20 w/w %.

Example 6: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0212] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of rhizoma curcumae longae, 80 parts of fructus Piperis longi, 60 parts of Dendrobium officinale, 60 parts of ginseng, 60 parts of mulberry, 40 parts of Radix puerariae, 40 parts of Cornus officinalis, 40 parts of Semen ziziphi spinosae, 40 parts of Radix astragali, 40 parts of peach kernel, 20 parts of licorice, and 20 parts of honeysuckle.

[0213] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0214] S1. a dried rhizoma curcumae longae product, a dried fructus Piperis longi product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Dendrobium officinale product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0215] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0216] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0217] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.35 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 3.47 w/w %.

Example 7: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0218] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 40 parts of rhizoma curcumae longae, 40 parts of fructus Piperis longi, 30 parts of Dendrobium officinale, 20 parts of ginseng, 20 parts of mulberry, 10 parts of Radix puerariae, 10 parts of Cornus officinalis, 10 parts of Semen ziziphi spinosae, 10 parts of Radix astragali, 10 parts of peach kernel, 5 parts of licorice, and 5 parts of honeysuckle.

[0219] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0220] S1. a dried rhizoma curcumae longae product, a dried fructus Piperis longi product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Dendrobium officinale product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 160° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0221] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 40% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:15) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0222] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0223] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.3 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 4.04 w/w %.

Example 8: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0224] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 50 parts of rhizoma curcumae longae, 50 parts of fructus Piperis longi, 40 parts of Dendrobium officinale, 40 parts of ginseng, 40 parts of mulberry, 25 parts of Radix puerariae, 20 parts of Cornus officinalis, 20 parts of Semen ziziphi spinosae, 20 parts of Radix astragali, 20 parts of peach kernel, 8 parts of licorice, and 8 parts of honeysuckle.

[0225] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0226] S1. a dried rhizoma curcumae longae product, a dried fructus Piperis longi product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Dendrobium officinale product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 170° C. and 3 MPa for 45 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0227] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 50% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:20) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0228] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.3 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0229] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.4 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 2.98 w/w %.

Example 9: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0230] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 60 parts of rhizoma curcumae longae, 50 parts of fructus Piperis longi, 50 parts of Dendrobium officinale, 30 parts of ginseng, 30 parts of mulberry, 40 parts of Radix puerariae, 18 parts of Cornus officinalis, 18 parts of Semen ziziphi spinosae, 30 parts of Radix astragali, 20 parts of peach kernel, 15 parts of licorice, and 15 parts of honeysuckle.

[0231] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0232] S1. a dried rhizoma curcumae longae product, a dried fructus Piperis longi product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Dendrobium officinale product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 165° C. and 3 MPa for 50 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0233] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step 51 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 48% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:20) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0234] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0235] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.4 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 3.21 w/w %.

Example 10: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0236] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of rhizoma curcumae longae, 60 parts of ginseng, 60 parts of mulberry, 40 parts of Radix puerariae, 40 parts of Cornus officinalis, 40 parts of Semen ziziphi spinosae, 40 parts of Radix astragali, 40 parts of peach kernel, 20 parts of licorice, and 20 parts of honeysuckle.

[0237] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0238] S1. a dried rhizoma curcumae longae product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0239] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0240] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0241] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.3 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 2.76 w/w %.

Example 11: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0242] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of rhizoma curcumae longae, 80 parts of fructus Piperis longi, 60 parts of ginseng, 60 parts of mulberry, 40 parts of Radix puerariae, 40 parts of Cornus officinalis, 40 parts of Semen ziziphi spinosae, 40 parts of Radix astragali, 40 parts of peach kernel, 20 parts of licorice, and 20 parts of honeysuckle.

[0243] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0244] S1. a dried rhizoma curcumae longae product, a dried fructus Piperis longi product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0245] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step 51 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0246] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.4 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0247] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.4 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 2.39 w/w %.

Example 12: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0248] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of rhizoma curcumae longae, 60 parts of Dendrobium officinale, 60 parts of ginseng, 60 parts of mulberry, 40 parts of Radix puerariae, 40 parts of Cornus officinalis, 40 parts of Semen ziziphi spinosae, 40 parts of Radix astragali, 40 parts of peach kernel, 20 parts of licorice, and 20 parts of honeysuckle.

[0249] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0250] S1. a dried rhizoma curcumae longae product, a dried Dendrobium officinale product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0251] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0252] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.4 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 Mpa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0253] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.4 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 3.41 w/w %.

Example 13: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0254] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 60 parts of Dendrobium officinale, 80 parts of fructus Piperis longi, 60 parts of ginseng, 60 parts of mulberry, 40 parts of Radix puerariae, 40 parts of Cornus officinalis, 40 parts of Semen ziziphi spinosae, 40 parts of Radix astragali, 40 parts of peach kernel, 20 parts of licorice, and 20 parts of honeysuckle.

[0255] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0256] S1. a dried Dendrobium officinale product, a dried fructus Piperis longi product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0257] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step 51 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0258] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0259] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.5 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 3.35 w/w %.

Comparative Example 1: Chinese Herbal Medicine Extract for Relieving Hearing Loss

[0260] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of fructus Piperis longi.

[0261] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0262] S1. a dried fructus Piperis longi product was crushed and sieved through a 60-mesh sieve, the medicinal fine powder was measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0263] S2. the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0264] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0265] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.5 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 2.62 w/w %.

Comparative Example 2: Chinese Herbal Medicine Extract for Relieving Hearing Loss

[0266] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 60 parts of Dendrobium officinale.

[0267] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0268] S1. a dried Dendrobium officinale product was crushed and sieved through a 60-mesh sieve, the medicinal fine powder was measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for late use;

[0269] S2. the medicine residue of step S1 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0270] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0271] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.5 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 4.11 w/w %.

Comparative Example 3: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0272] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 60 parts of ginseng, 60 parts of mulberry, 40 parts of Radix puerariae, 40 parts of Cornus officinalis, 40 parts of Semen ziziphi spinosae, 40 parts of Radix astragali, 40 parts of peach kernel, 20 parts of licorice, and 20 parts of honeysuckle.

[0273] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0274] S1. a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0275] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step 51 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0276] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.3 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0277] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.4 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 3.06 w/w %.

Comparative Example 4: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0278] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 80 parts of fructus Piperis longi, 60 parts of ginseng, 60 parts of mulberry, 40 parts of Radix puerariae, 40 parts of Cornus officinalis, 40 parts of Semen ziziphi spi by weight of licorice, and 20 parts of honeysuckle.

[0279] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0280] S1. a dried fructus Piperis longi product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0281] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step 51 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0282] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.4 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0283] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.4 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 3.76 w/w %.

Comparative Example 5: Chinese Herbal Medicine Composition for Relieving Hearing Loss

[0284] 1. A Chinese herbal medicine composition for relieving hearing loss was prepared from the following raw materials in parts by weight: 60 parts of Dendrobium officinale, 60 parts of ginseng, 60 parts of mulberry, 40 parts of Radix puerariae, 40 parts of Cornus officinalis, 40 parts of Semen ziziphi spinosae, 40 parts of Radix astragali, 40 parts of peach kernel, 20 parts of licorice, and 20 parts of honeysuckle.

[0285] 2. A preparation method of the Chinese herbal medicine composition for relieving hearing loss included the following steps:

[0286] S1. a dried Dendrobium officinale product, a dried mulberry product, a dried licorice product, a dried peach kernel product, a dried Semen ziziphi spinosae product and a dried honeysuckle product were separately crushed and sieved through a 60-mesh sieve, the medicinal fine powders were measured according to the formula amount and put into a subcritical extraction tank for steaming for 5 min with a water vapor, water at 15 times the mass of the medicinal materials was added, an extraction was conducted at 180° C. and 3 MPa for 60 min, after a temperature was lowered to a room temperature, filtration and centrifugation were conducted to obtain a water extract, the extraction was repeated once to obtain a filtrate, and a medicine residue was for later use;

[0287] S2. Radix puerariae, ginseng, Radix astragali and Cornus officinalis were crushed and sieved through a 60-mesh sieve, the medicinal materials were put into the extraction tank according to the formula amount, the medicine residue of step 51 was put into the extraction tank, an aqueous solution with ethanol at a concentration of 60% (a material-to-liquid ratio (g/mL) of the medicine residue to the solution was 1:25) was added, an extraction was conducted at 70° C. for 2 h, filtration was conducted, the extraction was repeated once, a filter residue was discarded, an extract was filtered with a tubular centrifuge (at 20,000 r/min) and a filtrate was collected for later use;

[0288] S3. the filtrates of steps S1 and S2 were mixed, an obtained mixture was concentrated at 65° C. and −0.5 Mpa to ⅕ of the volume of the mixture, and an obtained concentrated solution was subjected to a high-pressure homogenization at 100 MPa and 35° C. for 0.5 h for a total of 5 times to obtain a traditional Chinese medicine mixture; and

[0289] S4. the traditional Chinese medicine mixture of step S3 was concentrated at 65° C. and −0.4 MPa to a relative density of 1.30, and drying was conducted to obtain the Chinese herbal medicine composition with the moisture content of 4.45 w/w %.

Effect Examples

1. Effect of Chinese Herbal Medicine Extract/Chinese Herbal Medicine Composition on Lipopolysaccharide (LPS)-Induced Nitric Oxide Secretion in RAW264.7 Cells

[0290] After lipopolysaccharide (LPS) inducer acts on the cochlea, the LPS inducer stimulates and causes a series of inflammatory reactions in a cochlear tissue. Specifically, the LPS inducer induces cochlear hearing loss, at the same time promotes over expressions of inflammatory factors such as TNF-α and nitric oxide (NO), and induces an oxidation stress response of the cochlear tissue, and exacerbates hearing loss. Therefore, in a modeling, abnormal secretion of NO in inflammation can also reflect the degree of the hearing loss. RAW264.7 cells (RAW264.7 mouse macrophages) were inoculated on a 6-well plate at a density of 2×10.sup.6 cells/mL and cultured for 24 h until the cells adhered; a complete medium was used in a blank group, LPS with a final mass concentration of 2 mg/L was used in a model group, and a test sample (the Chinese herbal medicine extract/Chinese herbal medicine composition of examples 1 to 5 and comparative examples 1 to 2) with a final mass concentration of 100 μg/mL and 2 mg/L of LPS were separately used in each administration group (sample group). The total volume of each group was 2 mL and each group was put in a cell incubator to be cultured continuously for 24 h; and a cell supernatant was collected and measured according to the instructions of a NO detection kit, each experiment was independently repeated 3 times, and the inhibition ratio (IR) of the NO was calculated, where S was the NO content of each group.

IR (%)=(S.sub.model group−S.sub.sample group)/(S.sub.model group−S.sub.blank group)×100%

[0291] A synergic index of each component was calculated according to the content of the index value (NO) and the synergic index (Berenbaum index) was calculated as follows: a calculation formula of the reference (Berenbaum M C. The expected effect of a combination of agents: the general solution. Journal of Theoretical Biology, 1985, 114:413-431):

[00001]${.Math.}_{i=1}^n\frac{Xi}{Xie},$

where Xi referred to dosage of an i-th medicine when used in combination; Xie referred to dosage of the i-th medicine that can produce the same effect as the combination when used alone; n referred to the number of medicines used in combination; and when the Berenbaum index was less than 1, the medicine combination was synergistic and when the Berenbaum index was greater than 1, the medicine combination was antagonistic.

[0292] The experimental results were shown in Table 1: LPS induced abnormal NO secretion after acting on the RAW264.7 cells, and the NO content of the cells in the model group increased significantly, indicating that the model was successful; after the Chinese herbal medicine extract/Chinese herbal medicine composition prepared from at least one of rhizoma curcumae longae, Dendrobium officinale and fructus Piperis longi acted on the LPS-induced cells, the NO content of the cells was effectively reduced and cell inflammation was reduced, indicating that the Chinese herbal medicine extract/Chinese herbal medicine composition prepared from at least one of rhizoma curcumae longae, Dendrobium officinale and fructus Piperis longi had a good effect of relieving LPS-induced hearing loss. The mechanism may lie in reducing the degree of the hearing loss by inhibiting the inflammatory process. However, there was no disclosure of use of rhizoma curcumae longae in relieving aging hearing loss in the prior art. In addition, the Chinese herbal composition provided in examples 2 to 5 had the synergic index of less than 1, indicating that the Chinese herbal composition provided in examples 2 to 5 was not a simple combination, but had a synergistic anti-inflammatory effect.

TABLE-US-00001 TABLE 1 Synergistic effect of each component Experiemntal Content of Inhibition Synergic index group NO(μmol/L) ratio of NO (%) (SI*) Blank group 7.89 Model group 34.88 Example 1 group 24.39 38.87 Example 2 group 19.42 57.29 0.4028 Example 3 group 23.46 42.31 0.6818 Example 4 group 24.88 37.04 0.8934 Example 5 group 12.86 81.58 0.3475 Comparative 27.50 27.33 example 1 group Comparative 29.80 18.83 example 2 group Note: *referred to SI of the composition, a single raw material was used in example 1 and comparative examples 1-2 (the synergic index was not calculated), according to a determination index, SI value less than 1 indicated a synergistic effect produced by the composition, the smaller SI value meant that each component in the composition used at a lower dosage (concentration) can play an effect or exceed the effect produced by the same dosage of a single component (that is, the total concentration of the components of the composition), the better the synergistic effect presented, such that the combination of example 5 had the best synergistic effect, followed by a combination of rhizoma curcumae longae and fructus piperis longi (example 2), a combination of rhizoma curcumae longae and dendrobium officinale (example 3) and a combination of fructus piperis longi and dendrobium officinale (example 4).

2. Aging Hearing Loss

[0293] According to a method in the reference (Bai Xue, Liao Zhihang, Zhou Lijuan, et al. Effect of hearing-improving granules on hearing of experimental senile guinea pigs [J]. Chinese Traditional and Herbal Drugs, 2009, 40(5):777-779), a D-galactose-induced SD rat aging hearing loss model was used to evaluate the efficacy of the compositions of examples 1 to 5 and comparative examples 1 to 7. The specific method was as follows: the SD rats were adaptively reared for 7 days and divided into a blank group, a model group, a positive group (antideaf otic pills) and sample groups (example 5 to example 13, and comparative example 3 to comparative example 5), a total of 15 groups, each with 6 rats. Except for the blank group, the rats in the rest of the model group, the positive group and the sample groups were intraperitoneally injected with a D-galactose solution at a dosage of 500 mg/kg.Math.d separately, and the modeling lasted for 8 weeks. From the first day of the D-galactose modeling, the rats in the positive group and the sample groups were gavaged with a dosage of 100 mg/kg of rats (0.3 mL/100 g rat body weight per day), and the rats in the blank group and the model group were given gavaged with the same amount of normal saline. The rats were weighed every week. In the last week of the experiment, the ABR value of each group of rats was detected by an auditory brainstem response (ABR) instrument under a condition of 8 kHZ. On the last day of the eighth week, after the ABR value of each group of rats was detected by the ABR instrument under a condition of 8 kHZ, the rats were sacrificed and cochlear tissues of the rats in each group were taken, and the contents of the MDA and the glutamic acid (GLU) in the cochlear tissues were detected. The results were shown in Table 2. Compared with the blank group, the ABR value, the MDA, and the glutamic acid of the rats in the model group were significantly different (all p<0.001), indicating that the model was successful; the ABR value (p<0.05), the MDA (p<0.05), and the glutamic acid (p<0.05) of the rats in the example 5, 10, and 13 groups were significantly different through the regulation, the effect was good, and there was no disclosure of use of rhizoma curcumae longae in relieving aging hearing loss in the prior art; the ABR value (p<0.01), the MDA (p<0.01), and the glutamic acid (p<0.01) of the rats in examples 11 and 12 groups were significantly different through the regulation; the ABR value (p<0.001), the MDA (p<0.001), and the glutamic acid (p<0.001) of the rats in example 6 to 9 groups were significantly different through regulation, and the effect was good and significantly better than those of the rats in comparative examples 3 to 5; the ABR value (p<0.001) and the glutamic acid (p<0.001) of the rats in the positive group were significantly different, but the MDA showed no significant difference, indicating that the compositions of examples 6 to 9 had a significant better effect than that of the positive group; especially, the reduction value of the GLU of the rats in the example 6 to 9 groups (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi, Dendrobium officinale, ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice, and honeysuckle) (21.95, 20.17, 23.53 and 21.72 mg/L, respectively) relative to the model group was all higher than the sum of that of the rats in example 5 (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi and Dendrobium officinale) relative to the model group and that of the rats in comparative example 3 (the composition was prepared from ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice and honeysuckle) to the model group (11.13+5.67=16.8 mg/L), thus the compositions of examples 6 to 9 had a synergistic effect in reducing the GLU; and the composition of example 9 had a synergistic effect in reducing the ABR and the compositions of examples 6 and 9 had a synergistic effect in reducing the MDA.

TABLE-US-00002 TABLE 2 Experimental results of aging hearing loss of rats Experimental group ABRvalue (dB) MDA(U/mL) GLU(mg/L) Blank group 32.00 ± 3.38   5.30 ± 1.11 73.26 ± 1.39   Model group 44.71 ± 2.05.sup.###   .sup. 14.18 ± 2.82.sup.### 93.80 ± 2.12.sup.###  Positive group 34.98 ± 1.23*** 13.10 ± 1.58  73.00 ± 3.43*** Example 5 group 37.86 ± 3.18*  10.16 ± 2.51* 82.67 ± 2.13*  Example 6 group 33.29 ± 3.45***   5.44 ± 1.61*** 71.85 ± 2.77*** Example 7 group 33.43 ± 3.89***   7.32 ± 2.64*** 73.63 ± 2.95*** Example 8 group 34.71 ± 4.03***   6.95 ± 2.91*** 70.27 ± 3.93*** Example 9 group 32.03 ± 1.89***   6.27 ± 2.12*** 72.08 ± 2.29*** Example 10 group 39.02 ± 1.10*  10.03 ± 0.98* 86.54 ± 1.38*  Example 11 group 35.71 ± 1.31**   9.10 ± 0.83** 78.56 ± 1.39**  Example 12 group 36.28 ± 2.27**   8.27 ± 1.74** 76.85 ± 1.33**  Example 13 group 37.45 ± 2.03*   8.68 ± 2.39* 79.84 ± 2.41*  Comparative 40.01 ± 2.25   10.74 ± 1.47* 88.13 ± 1.79   example 3 group Comparative 38.56 ± 2.72*   9.86 ± 1.90* 83.07 ± 1.16*  example 4 group Comparative 39.89 ± 3.38   11.11 ± 0.42  85.72 ± 0.44*  example 5 group Note: .sup.###meant p < 0.001 compared to the blank group; ***meant p < 0.001 compared to the model group; **meant p < 0.01 compared to the model group; and *meant p < 0.05 compared to the model group.

3. Noise-Induced Hearing Loss

[0294] A noise modeling animal model was used for evaluation. The SD rats were adaptively reared for 7 days and divided into a blank group, a model group, a positive group (antideaf otic pills) and sample groups (example 5 to example 13, and comparative example 3 to comparative example 5), a total of 15 groups, each with 6 rats. Except for the blank group, the rats in the rest of the model group, the positive group and the sample groups were placed in cages with 6 SD rats in each cage and placed in an exposure chamber (with a soundproof box of 1.2 m long, 1.2 m wide and 1.7 m high). A white noise generator was used for sounding, an A-K200 power amplifier was used for amplification, and the noise was played to the exposure chamber by a speaker located on the top part of the exposure chamber. A sound pressure meter was used for continuous monitoring during exposure, the noise was maintained at a frequency of 20 Hz-20 kHz, an average sound intensity was a 90 dB sound pressure level, an unevenness of a sound field in an animal exposure range was (±2) dB, ventilation of the exposure chamber was ensured during the noise exposure, and the rats were given normal feed and normal drinking water and continuously exposed every day for 6 hours for a total of 14 d. During the modeling period, the rats in the positive group and the sample groups were gavaged with a dosage of 100 mg/kg of rats (0.3 mL/100 g rat body weight per day), and the rats in the blank group and the model group were given gavaged with the same amount of normal saline. At the end of the experiment (the 14th day), the ABR value of each group of rats was detected by the ABR instrument under a condition of 8 kHZ, after the detection, the cochlear tissues of the rats in each group were taken, and the contents of the MDA and the glutamic acid in the cochlear tissues were detected. The results were shown in Table 3. Compared with the blank group, the ABR value, the MDA, and the glutamic acid of the rats in the model group were significantly different (p<0.001/p<0.01), indicating that the model was successful; the ABR value (p<0.05), the MDA (p<0.05/p<0.01), and the glutamic acid (p<0.05/p<0.01) of the rats in the example 5 and 10 to 13 groups were significantly different through the regulation, the effect was good, and there was no disclosure of use of rhizoma curcumae longae in relieving noise-induced hearing loss in the prior art; the ABR value (p<0.001), the MDA (p<0.001), and the glutamic acid (p<0.001) of the rats in example 6 to 9 groups were significantly different through regulation, and the effect was good and significantly better than those of the rats in comparative examples 3 to 5; the ABR value (p<0.001), the glutamic acid (p<0.001) and the MDA (p<0.05) of the rats in the positive group were significantly different, but the compositions of examples 6 to 9 had a significant better effect than that of the positive group; especially, the reduction value of the MDA of the rats in the example 6 to 9 groups (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi, Dendrobium officinale, ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice, and honeysuckle) (17.63, 18.86, 17.53 and 20.7 U/mL respectively) relative to the model group was all higher than the sum of that of the rats in example 5 (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi and Dendrobium officinale) relative to the model group and that of the rats in comparative example 3 (the composition was prepared from ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice and honeysuckle) to the model group (8.95+6.18=15.13 U/mL), thus the compositions of examples 6 to 9 had a synergistic effect in reducing the MDA; and the compositions of examples, 6, 8 and 9 had a synergistic effect in reducing the ABR and the composition of example 9 had a synergistic effect in reducing the MDA.

TABLE-US-00003 TABLE 3 Experimental results of noise-induced hearing loss of rats Experimental group ABR value (dB) MDA(U/mL) GLU(mg/L) Blank group 28.57 ± 2.26  62.06 ± 2.48  62.52 ± 1.24   Model group  41.43 ± 5.80.sup.##  73.08 ± 3.12.sup.## 79.84 ± 1.91.sup.##  Positive group  32.14 ± 2.47*** 63.28 ± 1.86* 57.29 ± 3.44*** Example 5 group 34.29 ± 1.23* 64.13 ± 2.57* 66.63 ± 3.13*  Example 6 group  30.71 ± 3.19***  55.45 ± 1.81*** 59.35 ± 1.35*** Example 7 group  32.86 ± 1.52***  54.22 ± 3.32*** 55.34 ± 1.76*** Example 8 group  25.71 ± 1.75***  55.55 ± 1.43*** 57.99 ± 3.60*** Example 9 group  28.17 ± 1.13***  52.38 ± 2.32*** 54.12 ± 2.74*** Example 10 group 36.43 ± 1.25* 64.04 ± 2.08* 66.58 ± 1.81*  Example 11 group 34.59 ± 0.31*  61.45 ± 2.38** 62.57 ± 1.88**  Example 12 group 36.00 ± 1.11*  62.61 ± 0.98** 65.03 ± 4.08*  Example 13 group 35.18 ± 0.27* 63.20 ± 2.01* 63.45 ± 2.20**  Comparative 38.57 ± 3.39  66.90 ± 3.78  68.14 ± 4.19*  example 3 Comparative 35.12 ± 2.04* 67.49 ± 1.23  63.76 ± 2.78**  example 4 Comparative 37.06 ± 1.06  64.72 ± 3.00* 66.09 ± 3.39*  example 5 Note: .sup.###meant p < 0.001 compared to the blank group; .sup.##meant p < 0.01 compared to the blank group; ***meant p < 0.001 compared to the model group; **meant p < 0.01 compared to the model group; and *meant p < 0.05 compared to the model group.

4. Inflammatory Hearing Loss

[0295] The lipopolysaccharide (LPS) was used to induce an animal model of otitis media. After SD rats were adaptively reared for 7 days and the middle ear and inner ear of the rats were checked to confirm that there was no pathological changes, the rats were divided into a blank group, a model group, a positive group (antideaf otic pills) and sample groups (example 5 to example 13, and comparative example 3 to comparative example 5), a total of 15 groups, each with 6 rats. Except for the blank group, the rats in the rest groups were anesthetized with chloral hydrate, the head was fixed, the external ear canal was cleaned with ear microscopy forceps, after an ear endoscopy, the external ear canal was disinfected with 75% alcohol, the tympanic membrane was exposed by an electric otoscope, and the tympanic membrane was puncture at an anterior inferior position under a direct view, 20 μL of a lipopolysaccharide solution (5 mg/mL) was injected into the tympanic cavity with an insulin syringe for 7 consecutive days. During the modeling period, the rats in the positive group and the sample groups were gavaged with a dosage of 100 mg/kg of rats (0.3 mL/100 g rat body weight per day), and the rats in the blank group and the model group were given gavaged with the same amount of normal saline. At the end of the experiment (the 7.sup.th day), the ABR value of each group of rats was detected by the ABR instrument under a condition of 8 kHZ, after the detection, the cochlear tissues of the rats in each group were taken, and the contents of the MDA, the glutamic acid and IL-4 in the cochlear tissues were detected. The results were shown in Table 4. Compared with the blank group, the ABR value, the MDA, the glutamic acid and the IL-4 of the rats in the model group were significantly different (p<0.001, p<0.01), indicating that the model was successful; the ABR value (p<0.05/p<0.01), and/or the MDA (p<0.05), and/or the glutamic acid (p<0.05/p<0.01), and/or the IL-4 (p<0.05/p<0.01) of the rats in the example 5 and 10 to 13 groups were significantly different through the regulation, the effect was good, and there was no disclosure of use of rhizoma curcumae longae in relieving inflammatory hearing loss in the prior art; the ABR value (p<0.001), the MDA (p<0.001), the glutamic acid (p<0.001) and the IL-4 (p<0.001) of the rats in example 6 to 9 groups were significantly different through regulation, and the effect was good and significantly better than those of the rats in comparative examples 3 to 5; the ABR value (p<0.001), the glutamic acid (p<0.001) and the MDA (p<0.001) of the rats in the positive group were significantly different, but the IL-4 showed no significant difference, indicating the compositions of examples 6 to 9 had a significant better effect than that of the positive group; especially, the reduction value of the ABR of the rats in the example 6 to 9 groups (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi, Dendrobium officinale, ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice, and honeysuckle) (16.5, 16.5, 13.22 and 15.75 dB respectively) relative to the model group was all higher than the sum of that of the rats in example 5 (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi and Dendrobium officinale) relative to the model group and that of the rats in comparative example 3 (the composition was prepared from ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice and honeysuckle) to the model group (6.16+2.33=8.49 dB); the reduction value of the GLU of the rats in the example 6 to 9 groups (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi, Dendrobium officinale, ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice, and honeysuckle) (13.84, 19.08, 17.88 and 16.74 mg/L respectively) relative to the model group was all higher than the sum of that of the rats in example 5 (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi and Dendrobium officinale) relative to the model group and that of the rats in comparative example 3 (the composition was prepared from ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice and honeysuckle) to the model group (6.98+3.9=10.88 mg/L); the reduction value of the IL-4 of the rats in the example 6 to 9 groups (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi, Dendrobium officinale, ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice, and honeysuckle) (130.12, 166.45, 209.93 and 156.53 pg/mL respectively) relative to the model group was all higher than the sum of that of the rats in example 5 (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi and Dendrobium officinale) relative to the model group and that of the rats in comparative example 3 (the composition was prepared from ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice and honeysuckle) to the model group (38.67+51.36=90.03 pg/mL); thus the compositions of examples 6 to 9 had synergistic effects in reducing the ABR, GLU and IL-4.

TABLE-US-00004 TABLE 4 Experimental results of inflammatory hearing loss of rats Experimental group ABRvalue (dB) MDA(U/mL) GLU(mg/L) IL-4 (pg/mL) Blank group 41.50 ± 3.27   50.33 ± 2.19  76.67 ± 2.15  222.03 ± 29.01   Model group 57.83 ± 3.68.sup.##   68.47 ± 3.21.sup.##  .sup. 92.27 ± 2.34.sup.### 361.92 ± 27.91.sup.###  Positive group 40.43 ± 4.72***  56.56 ± 3.02**  75.03 ± 1.79*** 324.27 ± 11.17   Example 5 group 51.67 ± 2.19*  57.14 ± 3.77* 85.29 ± 1.98* 323.25 ± 35.89   Example 6 group 41.33 ± 3.15***  56.83 ± 2.76**  78.43 ± 3.00*** 231.80 ± 22.71*** Example 7 group 41.33 ± 2.25***  56.21 ± 2.21**  73.19 ± 2.31*** 195.47 ± 29.58*** Example 8 group 44.61 ± 3.98***  52.76 ± 2.02**  74.39 ± 3.24*** 151.99 ± 15.17*** Example 9 group 42.08 ± 2.21***  54.21 ± 3.16**  75.53 ± 2.53*** 205.39 ± 14.38*** Example 10 group 50.16 ± 4.91   59.09 ± 4.01* 82.88 ± 3.00* 300.98 ± 18.15*  Example 11 group 47.47 ± 2.55**  57.75 ± 3.25*  81.05 ± 2.19** 263.73 ± 22.77**  Example 12 group 53.11 ± 1.39   59.15 ± 2.47* 85.26 ± 2.58* 277.35 ± 19.43**  Example 13 group 46.61 ± 3.00**  57.00 ± 4.64*  80.37 ± 3.55** 254.80 ± 37.46**  Comparative 55.50 ± 4.64   61.12 ± 3.42* 88.37 ± 4.23* 310.56 ± 27.26   example 3 group Comparative 51.22 ± 2.23*  61.18 ± 1.47* 84.52 ± 4.11* 297.21 ± 20.71*  example 4 group Comparative 52.58 ± 1.09*  57.98 ± 2.06* 85.09 ± 2.25* 283.72 ± 30.61*  example 5 group Note: .sup.###meant p < 0.001 compared to the blank group; .sup.##meant p < 0.01 compared to the blank group; ***meant p < 0.001 compared to the model group; **meant p < 0.01 compared to the model group; and *meant p < 0.05 compared to the model group.

[0296] The above experimental results indicated that the Chinese herbal medicine extract/Chinese herbal composition for relieving hearing loss provided by the present disclosure had significant effects in relieving hearing loss such as aging hearing loss, noise-induced hearing loss, and inflammatory hearing loss. Especially, the compositions of examples 6 to 9 had better relieving effects (mean value or significance of the indexes) than those of the positive group and the comparative example groups. In particular, the compositions of examples 6 to 9 had better effects in improving the indexes of aging hearing loss, noise-induced hearing loss, and inflammatory hearing loss (ABR value and/or MDA and/or GLU and/or IL-4) relative to the model group than the sum of the effects of the composition of example 5 (the composition was prepared from rhizoma curcumae longae, fructus Piperis longi and Dendrobium officinale) relative to the model group and the composition of comparative example 3 (the composition was prepared from ginseng, mulberry, Radix puerariae, Cornus officinalis, Semen ziziphi spinosae, Radix astragali, peach kernel, licorice and honeysuckle) relative to the effect of the model group, indicating that the compositions of examples 6 to 9 had synergistic effects in improving the indexes of aging hearing loss, noise-induced hearing loss, and inflammatory hearing loss (ABR value and/or MDA and/or GLU and/or IL-4).

5. Taste

[0297] Sensory evaluation was performed on the Chinese herbal medicine extracts/Chinese herbal medicine compositions obtained in examples 5 to 13 and comparative examples 3 to 5 (a total of 30 sensory evaluation testers were included after qualified training). 0.5 g of the compositions of examples 5 to 13 and comparative examples 3 to 5 was separately added to 100 mL of water to be prepared into a 0.5% aqueous dispersion without containing any flavoring agents or auxiliary materials, and bitter and pungent tastes were tested. A rhizoma curcumae longae extract was obtained by a 60% ethanol water extraction process (Chinese herbal medicine extract in example 1) as a reference for a pungent control group. The specific results were shown in Table 5: the compositions obtained in examples 6 to 9 only had a little bitter and pungent tastes, thus the prescription combinations in examples 6 to 9 helped to improve the taste of the prescription extract powder.

TABLE-US-00005 TABLE 5 Taste test of compositions obtained in examples and comparative examples Experimental group Bitter taste Pungent taste Pungent control group − + + + + Example 5 group + + + + Example 6 group + − Example 7 group + + Example 8 group + − Example 9 group + − Example 10 group + + + + + Example 11 group + + + + + + Example 12 group + + + + Example 13 group + + + + Comparative + + + − example 3 Comparative + + + + + example 4 Comparative + + − example 5 Note: the mark ‘−’ meant that there was no obvious bitter or pungent taste; ‘+’ meant a little bitter or pungent taste; ‘+ + +’ meant relatively obvious bitter or pungent taste; and ‘+ + + +’ meant obvious bitter or pungent taste.

[0298] The examples are preferred embodiments of the present disclosure, but the embodiments of the present disclosure are not limited by the examples, and any other changes, modifications, substitutions, combinations and simplification made without departing from the spirit and principle of the present disclosure, all should be equivalent replacement methods and included in the protection scope of the present disclosure.