ANTI-INFLAMMATORY AND ANALGESIC COMPOUNDS AND USE THEREOF

Abstract

It belongs to the field of medicinal chemistry, in particular discloses anti-inflammatory and analgesic compounds and use thereof, the compounds of the present invention are compounds, isomers or pharmaceutically acceptable salts as shown in formula (I) or formula (II); the compounds have significant curative effect on gouty arthritis in rats, and have excellent anti-inflammatory factor effect; therefore, the compounds have potential application prospects in anti-inflammatory and analgesic drugs, especially in anti-acute gouty arthritis drugs, anti-rheumatoid arthritis drugs, drugs to reduce inflammatory factors, drugs to treat inflammatory storm or coronavirus pneumonia.

Claims

1. A compound has the structure shown in formula (I) or formula (II), or isomer and pharmaceutically acceptable salt thereof, ##STR00093## wherein, R.sup.1, R.sup.2, R.sup.3, or R.sup.4 are independently hydrogen, deuterium, halogen, cyano, C.sub.1-3 alkoxy, hydroxyl, aldehyde group, carboxyl, C.sub.2-6 ester, amide, substituted amide, C.sub.1-3 alkyl, substituted C.sub.1-3 alkoxy, or substituted C.sub.1-3 alkyl, respectively, the said substituent is one or more selected from the group consisting of deuterium, halogen, hydroxyl, carboxyl, C.sub.1-3 alkyl or cyano; alternatively, two adjacent groups in R.sup.1, R.sup.2, R.sup.3 and R.sup.4 are joined together to form OR.sup.17O group, wherein R.sup.17 is C.sub.1-6 alkyl; R.sup.5, R.sup.6, R.sup.7 or R.sup.8 are independently hydrogen, deuterium, halogen, hydroxyl, cyano, carboxyl, C.sub.2-6 ester group or substituted or non-substituted groups of amino, amide, C.sub.1-3 alkoxy or C.sub.1-3 alkyl, the said substituent is one or more selected from the group consisting of deuterium, halogen, cyano, C.sub.1-3 alkyl or C.sub.1-3 haloalkyl; R.sup.9 is hydrogen, C.sub.1-4 alkyl, or C.sub.1-4 substituted alkyl, the said substituent is one or more selected from the group consisting of deuterium, hydroxyl, C.sub.1-2 alkoxy, or cyano; X is C(O), S(O).sub.2, S(O), C(NH) or substituted C(NH), the substituent is selected from CN and SO.sub.2NH.sub.2; R.sup.10 is C.sub.1-4 alkyl, substituted C.sub.1-4 alkyl, C.sub.3-6 cycloalkyl, substituted C.sub.3-6 cycloalkyl, amino, C.sub.1-4 alkylamino, substituted C.sub.1-4 alkylamino, C.sub.3-6 cycloalkylamino, C.sub.1-4 alkoxy, substituted C.sub.1-4 alkoxy, or vinyl; or R.sup.9 is attached to R.sup.10 so that R.sup.9NXR.sup.10 forms a ring group together; the said substituent is selected from one or more of deuterium, halogen and cyano; R.sup.11 is hydrogen, deuterium, halogen, cyano, C.sub.1-2 alkyl, C.sub.1-2 alkoxy, carboxyl, or C.sub.1-3 alkyl substituted amide; R.sup.12 is a hydrogen, deuterium, halogen, hydroxyl, cyano, carboxyl, or substituted or non-substituted group of the following: C.sub.1-4 alkyl, C.sub.3-6 cycloalkyl, C.sub.1-4 alkoxy, morpholinyl, aminocarbonyl, C.sub.1-4 alkylamino carbonyl, or C.sub.1-4 alkoxy carbonyl; the substituent is selected from one or more of deuterium, halogen or cyano; and (a) when R.sup.12 is hydrogen, R.sup.13 is cyano, aldehyde group, carboxyl, borono, substituted C.sub.1-4 alkoxy, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, amino carbonyl, phenyl amino carbonyl, pyridinyl amino carbonyl, C.sub.1-4 alkylamino carbonyl, C.sub.1-4 alkoxy carbonyl, C.sub.1-4 alkylamino carbonyl amino, C.sub.2-6 heterocycloalkyl carbonyl, C.sub.3-6 heterocycloalkyl ketone group, C.sub.1-2 alkyl carbonyl, sulfonamido, sulfonyloxy, amino sulfonyl, amino sulfonyloxy, C.sub.1-4 alkyl sulfonamido, C.sub.1-4 alkyl amino sulfonyl, C.sub.1-4 alkyl amino sulfonamido, C.sub.1-4 alkyl sulfonyloxy, C.sub.1-4 alkyl amino sulfonyloxy, C.sub.1-4 alkyl amino carbonyloxy, tetrazolyl, triazolyl, imidazolyl, morpholinyl, C.sub.1-4 alkyl thio, C.sub.3-6 cycloalkoxy or C.sub.3-6 heterocycloalkyloxy, the substituent is selected from one or more of deuterium, halogen, cyano, hydroxyl, phenyl, pyridyl, C.sub.1-3 alkyl, C.sub.1-3 deuteroalkyl or C.sub.1-3 haloalkyl; (b) when R.sup.12 is not hydrogen, R.sup.13 is cyano, aldehyde group, carboxyl, borono, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, amino carbonyl, phenyl amino carbonyl, pyridinyl amino carbonyl, C.sub.1-4 alkylamino carbonyl, C.sub.1-4 alkoxy carbonyl amino, C.sub.1-4 alkylamino carbonyl amino, C.sub.2-6 heterocycloalkyl carbonyl, C.sub.3-6 heterocycloalkyl ketone group, C.sub.1-4 alkyl carbonyl, sulfonamido, sulfonyloxy, sulfamoyl, sulfamoyloxy, C.sub.1-4 alkyl sulfonamido, C.sub.1-4 alkyl amino sulfonyl, C.sub.1-4 alkyl amino sulfonamido, C.sub.1-4 alkyl sulfonyloxy, C.sub.1-4 alkyl amino sulfonyloxy, C.sub.1-4 alkyl amino carbonyloxy, heterocyclic group, C.sub.1-4 alkyl amino, C.sub.3-6 cycloalkyl amino, C.sub.3-6 heterocycloalkyloxy, C.sub.3-6 heterocycloalkyl amino, C.sub.1-4 alkyl carbonyl amino, C.sub.1-4 alkoxy, C.sub.1-4 alkylthio, C.sub.3-6 cycloalkoxy, C.sub.3-6 heterocyclo alkyl or C.sub.1-4 alkyl, the substituent is selected from one or more of deuterium, halogen, cyano, hydroxyl, phenyl, pyridyl, C.sub.1-3 alkyl, C.sub.1-3 deuteroalkyl and C.sub.1-3 haloalkyl; R.sup.14 is a cyano, carboxyl, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, amino carbonyl, C.sub.1-4 alkyl amino carbonyl, C.sub.1-4 alkoxy carbonyl amino, C.sub.1-4 alkyl amino carbonyl amino, C.sub.2-6 heterocycloalkyl carbonyl, sulfamoyl, C.sub.1-4 alkylaminosulfonyl, heterocyclic, C.sub.3-6 heterocycloalkyl, C.sub.3-6 heterocycloalkyloxy, C.sub.1-4 alkoxy, C.sub.3-6 cycloalkoxy, C.sub.3-6 heterocycloalkoxy or C.sub.1-4 alkyl, the substituent is selected from one or more of deuterium, halogen, cyano, hydroxyl, C.sub.1-3 alkyl and C.sub.1-3 haloalkyl; R.sup.15 is hydrogen, deuterium, halogen, cyano, C.sub.1-2 alkyl, C.sub.1-2 alkoxy, carboxyl, or C.sub.1-3 alkyl substituted amide; provided that the following conditions are excluded: (i) In formula (I), when R.sup.1-R.sup.3 is methoxy, R.sup.4-R.sup.9 is hydrogen, R.sup.10 is methyl, R.sup.11-R.sup.12 is hydrogen, and R.sup.15 is hydrogen, R.sup.13 is selected from the following groups: C.sub.1-4 alkoxy, C.sub.1-4 alkylthio, deutero C.sub.1-4 alkylthio, C.sub.1-4-alkoxy carbonyl amino, butyl carbonyl, or morpholinyl; (ii) In formula (I), R.sup.1-R.sup.3 is methoxy, R.sup.4-R.sup.9 is hydrogen, R.sup.10 is deuteromethyl, R.sup.11 is hydrogen, R.sup.12 is deuterium, R.sup.13 is methoxy, and R.sup.15 is deuterium; (iii) In formula (I), R.sup.1-R.sup.3 is methoxy, R.sup.4-R.sup.9 is hydrogen, R.sup.10 is methyl, R.sup.11 is hydrogen, R.sup.12 is bromine, R.sup.13 is methoxy, and R.sup.15 is bromine.

2. The compound according to claim 1, wherein the compound has the structure shown in formula (III) or formula (IV), ##STR00094## R.sup.1, R.sup.2, R.sup.3, or R.sup.4 are independently hydrogen, deuterium, halogen, cyano, C.sub.1-3 alkoxy, aldehyde group, C.sub.1-3 alkyl, substituted C.sub.1-3 alkoxy, or substituted C.sub.1-3 alkyl, respectively, wherein the substituent is one or more selected from the group consisting of deuterium, halogen, C.sub.1-3 alkyl, and cyano; alternatively, R.sup.1 and R.sup.2, R.sup.2 and R.sup.3, or R.sup.3 and R.sup.4 are joined together to form the OR.sup.17O group, wherein R.sup.17 is C.sub.1-3 alkyl; R.sup.5, R.sup.6, R.sup.7, or R.sup.8 is independently hydrogen, deuterium, halogen, cyano, carboxyl, hydroxyl, or amino, respectively; R.sup.9 is hydrogen, C.sub.1-4 alkyl, or C.sub.1-4 substituted alkyl, wherein the substituent is one or more selected from the group consisting of deuterium, hydroxyl, C.sub.1-2 alkoxy, and cyano; X is C(O) or S(O).sub.2 group; R.sup.10 is a C.sub.1-4 alkyl, substituted C.sub.1-4 alkyl, C.sub.3-6 cycloalkyl, substituted C.sub.3-6 cycloalkyl, amino, C.sub.1-4-alkyl amino, substituted C.sub.1-4-alkyl amino, C.sub.3-6 cycloalkyl amino, C.sub.1-4 alkoxy, substituted C.sub.1-4 alkoxy, or vinyl, or R.sup.9NXR.sup.10 forms ##STR00095## the substituent is selected from one or more of deuterium and cyano; R.sup.11 is hydrogen, deuterium, halogen, cyano, C.sub.1-2 alkyl, C.sub.1-2 alkoxy, carboxyl, or C.sub.1-3 alkyl substituted amide; R.sup.12 is hydrogen, deuterium, halogen, hydroxyl, cyano, or substituted or non-substituted group of the following: C.sub.1-4 alkyl, C.sub.3-6 cycloalkyl, C.sub.1-4 alkoxy, morpholinyl, C.sub.1-4 alkylamino carbonyl or C.sub.1-4-alkoxy carbonyl; the substituent is selected from one or more of deuterium, halogen and cyano; and (a) when R.sup.12 is hydrogen, R.sup.13 is a cyano, aldehyde group, carboxyl, borono, substituted C.sub.1-4 alkoxy, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, amino carbonyl, phenyl amino carbonyl, pyridinyl amino carbonyl, C.sub.1-4 alkyl amino carbonyl, C.sub.1-4 alkoxy carbonyl amino, C.sub.1-4 alkyl amino carbonyl amino, pyrrolidyl carbonyl, piperazinyl carbonyl, morpholinyl carbonyl, pyrrolidonyl, oxazolidinonyl, morpholinonyl, C.sub.1-2 alkyl carbonyl, sulfonamido, sulfonyloxy, amino sulfonyl, amino sulfonyloxy, C.sub.1-4 alkyl sulfonamido, C.sub.1-4 alkyl amino sulfonyl, C.sub.1-4 alkyl amino sulfonamido, C.sub.1-4 alkyl amino sulfonyloxy, C.sub.1-4 alkyl amino carbonyloxy, tetrazolyl, triazolyl, imidazolyl, morpholinyl, C.sub.1-4 alkyl thio, C.sub.3-6 cycloalkyloxy or tetrahydrofuranyloxy, the substituent is one or more selected from the group consisting of deuterium, halogen, cyano, hydroxyl, phenyl, C.sub.1-3 alkyl, C.sub.1-3 deuteroalkyl or C.sub.1-3 haloalkyl; (b) when R.sup.12 is not hydrogen, R.sup.13 is cyano, aldehyde group, carboxyl, borono, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, amino carbonyl, phenyl amino carbonyl, pyridinyl amino carbonyl, C.sub.1-4 alkyl amino carbonyl, C.sub.1-4 alkoxy carbonyl amino, C.sub.1-4 alkyl amino carbonyl amino, C.sub.2-6 heterocycloalkyl carbonyl, C.sub.3-6 heterocycloalkylketone group, C.sub.1-4 alkyl carbonyl, sulfonamido, sulfonyloxy, amino sulfonyl, amino sulfonyloxy, C.sub.1-4 alkyl sulfonamido, C.sub.1-4 alkyl amino sulfonyl, C.sub.1-4 alkyl amino sulfonamido, C.sub.1-4 alkyl sulfonyloxy, C.sub.1-4 alkyl amino sulfonyloxy, C.sub.1-4 alkyl amino carbonyloxy, heterocyclic, C.sub.1-4 alkyl amino, C.sub.3-6 cycloalkyl amino, tetrahydrofuranyloxy, tetrahydrofuranyl amino, C.sub.1-4 alkyl carbonyl amino, C.sub.1-4 alkoxy, C.sub.1-4 alkylthio, C.sub.3-6 cycloalkoxy, C.sub.3-6 heterocycloalkyl or C.sub.1-4 alkyl, wherein the substituent is one or more selected from the group consisting of deuterium, halogen, cyano, hydroxyl, phenyl, C.sub.1-3 alkyl, C.sub.1-3 deuteroalkyl or C.sub.1-3 haloalkyl; R.sup.14 is a cyano, carboxyl, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, aminocarbonyl, C.sub.1-4 alkyl amino carbonyl, C.sub.1-4 alkoxy carbonyl amino, C.sub.1-4 alkyl amino carbonyl amino, N-pyrrolidinyl carbonyl, amino sulfonyl, C.sub.1-4 alkyl amino sulfonyl, heterocyclic group, tetrahydrofuranyloxy, C.sub.1-4 alkoxy, C.sub.3-6 cycloalkyloxy, or C.sub.1-4 alkyl, wherein the substituent is one or more selected from the group consisting of deuterium, halogen, cyano, hydroxyl, C.sub.1-3 alkyl or C.sub.1-3 haloalkyl; R.sup.15 is hydrogen, deuterium, halogen, cyano, C.sub.1-2 alkyl, C.sub.1-2 alkoxy, carboxyl, or C.sub.1-3 alkyl substituted amide.

3. The compound according to claim 2, wherein the compound has the structure shown in formula (V) or formula (VI), ##STR00096## R.sup.1, R.sup.2 or R.sup.3 is independently C.sub.1-3 alkoxy respectively; or, R.sup.1 and R.sup.2 or R.sup.2 and R.sup.3 are joined together to form OR.sup.17O group, wherein R.sup.17 is C.sub.1-2 alkyl; R.sup.4 is hydrogen, deuterium, halogen, or cyano; R.sup.9 is hydrogen or C.sub.1-3 alkyl; X is C(O) or S(O).sub.2 group; R.sup.10 is C.sub.1-3 alkyl, substituted C.sub.1-3 alkyl, C.sub.3-6 cycloalkyl, amino, C.sub.1-4 alkyl amino, C.sub.1-3 alkoxy or vinyl, or R.sup.9 forms ##STR00097## group with NXR.sup.10; wherein the substituent is selected from one or more of deuterium and cyano; R.sup.11 is hydrogen, deuterium or halogen; R.sup.12 is hydrogen, deuterium, halogen, cyano, or substituted or non-substituted group of the following: C.sub.1-3 alkyl, C.sub.3-5 cycloalkyl, C.sub.1-3 alkoxy, morpholinyl, C.sub.1-4 alkylamino carbonyl or C.sub.1-4-alkoxy carbonyl; wherein the substituent is selected from one or more of deuterium and halogen; and (a) when R.sup.12 is hydrogen, R.sup.13 is a cyano, aldehyde group, carboxyl, borono, substituted C.sub.1-4 alkoxy, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, amino carbonyl, phenyl amino carbonyl, pyridinyl amino carbonyl, C.sub.1-4 alkyl amino carbonyl, C.sub.1-4 alkoxy carbonyl amino, C.sub.1-4 alkyl amino carbonyl amino, N-pyrrolidyl carbonyl, piperazinyl carbonyl, morpholinyl carbonyl, pyrrolidonyl, oxazolidinonyl, 3-morpholinonyl, C.sub.1-2 alkyl carbonyl, sulfonamido, sulfonyloxy, amino sulfonyl, amino sulfonyloxy, C.sub.1-4 alkyl sulfonamido, C.sub.1-4 alkyl amino sulfonyl, C.sub.1-4 alkyl amino sulfonamido, C.sub.1-4 alkyl amino sulfonyloxy, C.sub.1-4 alkyl amino carbonyloxy, tetrazolyl, triazolyl, imidazolyl, pyrazolyl, morpholinyl, C.sub.1-4 alkyl thio, C.sub.3-6 cycloalkyloxy or tetrahydrofuranyloxy, the substituent is one or more selected from the group consisting of deuterium, halogen, cyano, hydroxyl, phenyl, pyridyl, C.sub.1-3 alkyl, C.sub.1-3 deuteroalkyl or C.sub.1-3 haloalkyl; (b) when R.sup.12 is not hydrogen, R.sup.13 is cyano, aldehyde group, carboxyl, borono, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, amino carbonyl, phenyl amino carbonyl, pyridinyl amino carbonyl, C.sub.1-4 alkyl amino carbonyl, C.sub.1-4 alkoxy carbonyl amino, C.sub.1-4 alkyl amino carbonyl amino, pyrrolidonyl, 2-oxazolidinonyl, 3-morpholinonyl, pyrrolidinyl carbonyl, methylpiperazinyl carbonyl, morpholinyl carbonyl, C.sub.1-4 alkyl carbonyl, sulfonamido, sulfonyloxy, amino sulfonyl, amino sulfonyloxy, C.sub.1-4 alkyl sulfonamido, C.sub.1-4 alkyl amino sulfonyl, C.sub.1-4 alkyl amino sulfonamido, C.sub.1-4 alkyl sulfonyloxy, C.sub.1-4 alkyl amino sulfonyloxy, C.sub.1-4 alkyl amino carbonyloxy, heterocyclic, C.sub.1-4 alkyl amino, C.sub.3-6 cycloalkyl amino, tetrahydrofuranyloxy, tetrahydrofuranyl amino, C.sub.1-4 alkyl carbonyl amino, C.sub.1-4 alkoxy, C.sub.1-4 alkylthio, C.sub.3-6 cycloalkoxy, C.sub.3-6 heterocycloalkyl or C.sub.1-4 alkyl, wherein the substituent is one or more selected from the group consisting of deuterium, halogen, cyano, hydroxyl, phenyl, pyridyl, C.sub.1-3 alkyl, C.sub.1-3 deuteroalkyl or C.sub.1-3 haloalkyl; R.sup.14 is a cyano, carboxyl, or substituted or non-substituted group of C.sub.1-4 alkoxy carbonyl, aminocarbonyl, C.sub.1-4 alkyl amino carbonyl, C.sub.1-4 alkoxy carbonyl amino, C.sub.1-4 alkyl amino carbonyl amino, N-pyrrolidinyl carbonyl, amino sulfonyl, C.sub.1-4 alkyl amino sulfonyl, pyrrolidyl, oxazolidyl, isooxazolidyl, piperazinyl, morpholinyl, piperidyl, tetrahydrofuranyl, tetrahydrothiophenyl, hexahydropyrimidyl, tetraazolidyl, triazolidyl, tetrahydrofuranyloxy, C.sub.1-4 alkoxy, C.sub.3-6 cycloalkyloxy, or C.sub.1-4 alkyl, wherein the substituent is one or more selected from the group consisting of deuterium, halogen, cyano, hydroxyl, C.sub.1-3 alkyl or C.sub.1-3 haloalkyl; R.sup.15 is hydrogen, deuterium, halogen, methyl, ethyl, halomethyl or haloethyl.

4. The compound according to claim 1, wherein, R.sup.9 is hydrogen, methyl or ethyl; X is C(O) or S(O).sub.2 group; R.sup.10 is methyl, ethyl, cyclopropyl, amino, methylamino, ethylamino, dimethylamino, diethylamino, methoxy, ethoxy, cyanomethyl, or cyanoethyl, or R.sup.9 is joined together with NXR.sup.10 to form ##STR00098##

5. The compound claim 1, wherein, R.sup.12 is hydrogen, deuterium, methyl, ethyl, halogen, cyano, methoxy, ethoxy, morpholinyl, aminocarbonyl, phenyl aminocarbonyl, methyl aminocarbonyl, methyl formate or ethyl formate; and (a) when R.sup.12 is hydrogen, R.sup.13 is cyano, aldehyde group, carboxyl, borono, halomethoxyl, haloethoxyl, deutero methoxyl, deuteroethoxyl, methoxy carbonyl, ethoxy carbonyl, aminocarbonyl, phenyl aminocarbonyl, pyridinyl aminocarbonyl, methyl aminocarbonyl, deuteromethylaminocarbonyl, halomethyl aminocarbonyl, ethyl aminocarbonyl, dimethylaminocarbonyl, methoxy carbonyl amino, ethoxy carbonyl amino, (methoxy carbonyl)(methyl) amino, methyl amino carbonyl amino, ethyl amino carbonyl amino, dimethyl amino carbonyl, pyrrolidyl carbonyl, cyanopyrrolidyl carbonyl, piperazinyl carbonyl, methyl piperazinyl carbonyl, morpholinyl carbonyl, pyrrolidonyl, 2-oxazolidonyl, 3-morpholinonyl, methyl carbonyl, ethyl carbonyl, sulfonyl amino, sulfonyloxy, methyl sulfonyloxy, ethyl sulfonyloxy, hydroxyl sulfonyloxy, amino sulfonyl, amino sulfonyloxy, methyl sulfonyl amino, ethyl sulfonyl amino, methyl amino sulfonyl, methyl amino sulfonyl amino, methyl amino sulfonyloxy, N-ethyl methyl amino carbonyloxy, tetrazolyl, triazolyl, imidazolyl, pyrazolyl, morpholinyl, methylthio, ethylthio, halomethylthio, haloethylthio, deuteromethylthio, deuteroethylthio or tetrahydrofuranyloxy; (b) when R.sup.12 is not hydrogen, R.sup.13 is cyano, aldehyde group, carboxyl, borono, methoxy carbonyl, ethoxy carbonyl, methylaminocarbonyl, dimethylaminocarbonyl, aminocarbonyl, phenylamino carbonyl, pyridyl amino carbonyl, ethyl aminocarbonyl, halomethyl aminocarbonyl, deuteromethyl aminocarbonyl, (methoxycarbonyl)(methyl) amino, methyl aminocarbonyl amino, ethyl aminocarbonyl amino, dimethyl amino carbonyl amino, pyrrolidyl carbonyl, cyanopyrrolidyl carbonyl, piperazinyl carbonyl, methyl piperazinyl carbonyl, morpholinyl carbonyl, pyrrolidonyl, 2-oxazolidinonyl, 3-morpholinonyl, methyl carbonyl, ethyl carbonyl, sulfonyl amino, sulfonyloxy, hydroxyl sulfonyloxy, amino sulfonyl, amino sulfonyloxy, methyl sulfonyl amino, ethyl sulfonyl amino, methyl amino sulfonyl, ethyl amino sulfonyl, methyl amino sulfonyl amino, methyl amino sulfonyloxy, N-ethyl methyl amino carboxyloxy, tetrazolyl, triazolyl, methyl amino, ethyl amino, tetrahydrofuranyloxy, tetrahydrofuranyl amino, methyl carbonyl amino, methoxy, halomethoxy, deuteromethoxy, ethoxy, haloethoxy, deuteroethoxy, methylthio, halomethylthio, deuteromethylthio, ethylthio, haloethylthio, deuteroethylthio, tetrahydrofuranyl, piperazinyl, piperidyl, methylpiperidyl, imidazolyl, pyrazolyl, morpholinyl, pyrrolidinyl, tetrahydrocarbazolyl, morpholinyl, methyl, ethyl, propyl, hydroxymethyl, hydroxyethyl, halomethyl or haloethyl.

6. The compound according to claim 1, wherein, R.sup.11 is hydrogen or deuterium; R.sup.14 is methylaminocarbonyl, dimethylaminocarbonyl, cyano, methoxy carbonyl, ethoxy carbonyl, carboxyl, N-pyrrolidyl carbonyl, tetrahydrofuranyloxy, methoxy, ethoxy, cyclopropoxy, halomethoxy, haloethoxy, deuteromethoxy, deuteroethoxy, methyl, ethyl, propyl, hydroxymethyl, hydroxyethyl, tetraazolyl, triazolyl, morpholinyl, halomethyl or haloethyl; R.sup.15 is hydrogen, deuterium, methyl, ethyl, halomethyl, or haloethyl.

7. The compound according to claim 1, wherein the compounds are selected from the group consisting of: ##STR00099## ##STR00100## ##STR00101## ##STR00102## ##STR00103## ##STR00104## ##STR00105## ##STR00106## ##STR00107## ##STR00108## ##STR00109## ##STR00110## ##STR00111## ##STR00112## ##STR00113## ##STR00114## ##STR00115## ##STR00116## ##STR00117## ##STR00118##

8. A pharmaceutical composition comprising the compounds claim 1 as active ingredient, supplemented by pharmaceutically acceptable excipients.

9. The pharmaceutical composition according to claim 8, wherein the pharmaceutical composition is used as anti-inflammatory or analgesic drugs.

10. A method for treating a disease comprising a step of administering the compound of claim 1 to a subject in need, wherein the disease is selected from the group consisting of arthritis, acute gouty arthritis, rheumatoid arthritis, inflammatory storms and coronavirus pneumonia.

Description

SPECIFIC MODE FOR CARRYING OUT THE EMBODIMENTS

[0133] The present invention is further illustrated in combination with Examples below, but the scope of protection of the present invention is not limited to the following Examples.

Example 1: Synthesis of (S)N-(1,2,3-trimethoxy-10-methanesulfonylamino-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (2)

##STR00027##

[0134] Step A: A mixture containing colchicine (800 mg, 2.0 mmol), concentrated ammonia (15 mL) and methanol (5 mL) was stirred at 30 C. for 40 hours. Saturated saline (30 mL) was added, and the resulting mixture was extracted with ethyl acetate (40 mL4) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure to give (S)N-(10-amino-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (1) (699 mg). The yield was 90.9%.

[0135] Step B: To a solution of compound 1 (140 mg, 0.364 mmol) in pyridine (7 mL) was added methanesulfonyl chloride (9 drops), and after addition, the resulting mixture was stirred at 55 C. for 3 hours. The solvent was evaporated under reduced pressure, water (20 mL) was added, the product was extracted with ethyl acetate (30 mL3), and the combined organic phases were washed with saturated brine (20 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with petroleum ether:ethyl acetate=1:11:6) to give (S)N-(1,2,3-trimethoxy-10-methanesulfonylamino-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (2) (101 mg). The yield was 60.0%. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 9.72 (s, 1H), 8.81 (d, J=10.8 Hz, 1H), 8.65 (d, J=6.8 Hz, 1H), 7.34 (s, 1H), 7.27 (d, J=10.8 Hz, 1H), 6.80 (s, 1H), 4.41-4.35 (m, 1H), 3.86 (s, 3H), 3.81 (s, 3H), 3.55 (s, 3H), 2.65-2.61 (m, 1H), 2.28-2.18 (m, 4H), 2.10-2.04 (m, 1H), 1.93-1.81 (m, 4H). MS (ESI, m/z): 461.1 [MH].sup..

Example 2: Synthesis of (S)N-(4-chloro-1,2,3,10,11-pentamethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (4)

##STR00028##

[0136] Step A: A mixture containing colchicine (3.0 g, 7.51 mmol), NCS (1.30 g, 9.73 mmol) and acetic acid (30 mL) was stirred under nitrogen at 70 C. for 3.5 hours. Most of the solvent was evaporated under reduced pressure, water (60 mL) was added, the product was extracted with ethyl acetate (60 mL3), the combined organic phases were washed with saturated brine (40 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with ethyl acetate:methanol=40:110:1) to afford (S)N-(4-chloro-1,2,3,10-tetramethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (3) (2.50 g). The yield was 76.7%.

[0137] Step B: A mixture containing compound 3 (2.70 g, 6.22 mmol), iodine (2.39 g, 9.42 mmol), silver nitrate (1.60 g, 9.42 mmol) and methanol (30 mL) was stirred at 30 C. overnight. Water (90 mL) was added and then saturated sodium thiosulfate solution was added dropwise until the reddish brown color disappeared. Extracted with ethyl acetate (60 mL3), the combined organic phases were washed with saturated brine (40 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with ethyl acetate:methanol=40:110:1) to give a yellow solid (1.48 g). The solid (600 mg) was taken and dissolved in DMF (6 mL), then cuprous cyanide (144 mg, 1.61 mmol) was added, and the resulting mixture was stirred at 100 C. for 3 hours. Water (25 mL) was added and the product was extracted with ethyl acetate (30 mL3), the combined organic phases were washed sequentially with water (15 mL2) and saturated saline (15 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with ethyl acetate:methanol=50:120:1) to afford (S)N-(4-chloro-1,2,3,10,11-pentamethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (4) (240 mg). The yield was 20.5%. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.63 (d, J=7.6 Hz, 1H), 7.18 (s, 1H), 7.08 (s, 1H), 4.19-4.12 (m, 1H), 3.91 (s, 3H), 3.87 (s, 3H), 3.86 (s, 3H), 3.80 (s, 3H), 3.58 (s, 3H), 3.13-3.08 (m, 1H), 2.18-2.10 (m, 1H), 1.98-1.89 (m, 1H), 1.84-1.79 (m, 4H). MS (ESI, m/z): 464.1 [M+H].sup.+.

Example 3: Synthesis of (S)N-(4-bromo-1,2,3,10,11-pentamethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (6)

##STR00029##

[0138] The experimental operations for synthesizing compound 6 was described in Example 2, wherein NCS in step A of Example 2 was replaced with NBS. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.63 (d, J=7.2 Hz, 1H), 7.19 (s, 1H), 7.10 (s, 1H), 4.20-4.13 (m, 1H), 3.93 (s, 3H), 3.89 (s, 3H), 3.88 (s, 3H), 3.83 (s, 3H), 3.61 (s, 3H), 3.16-3.12 (m, 1H), 2.30-2.22 (m, 1H), 1.98-1.76 (m, 5H). MS (ESI, m/z): 508.0 [M+H].sup.+.

Example 4: Synthesis of (S)N-(4-bromo-10,11-diethoxy-1,2,3,-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (7) and (S)N-(10,11-diethoxy-1,2,3,-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (8)

##STR00030##

[0139] Step A: The experimental operations of synthesizing compound 7 using compound 5 as raw material were described in Step B of Example 2, wherein methanol and silver nitrate in Step B of Example 2 were replaced with ethanol and silver sulfate, respectively. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.69 (d, J=7.2 Hz, 1H), 7.18 (s, 1H), 7.14 (s, 1H), 4.26-4.08 (m, 5H), 3.98 (s, 3H), 3.92 (s, 3H), 3.63 (s, 3H), 3.20-3.16 (m, 1H), 2.34-2.26 (m, 1H), 2.00-1.91 (m, 1H), 1.88 (s, 3H), 1.86-1.81 (m, 1H), 1.39-1.32 (m, 6H). MS (ESI, m/z): 536.0 [M+H].sup.+.

[0140] Step B: Diisopropylethylamine (120 mg, 0.929 mmol) and tetrakis(triphenylphosphine)palladium (93 mg, 0.0805 mmol) were added to a mixture containing Compound 7 (200 mg, 0.373 mmol), ethanol (4 mL), water (2 mL) and toluene (12 mL), and after addition, the resulting mixture was stirred at reflux under nitrogen overnight. Water (20 mL) was added and the product was extracted with ethyl acetate (20 mL3). The combined organic phases were washed sequentially with water (15 mL2) and saturated saline (10 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with ethyl acetate:methanol=100:130:1) to give (S)N-(10,11-diethoxy-1,2,3,-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (8). .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.57 (d, J=7.6 Hz, 1H), 7.10 (s, 1H), 7.09 (s, 1H), 6.78 (s, 1H), 4.29-4.07 (m, 5H), 3.84 (s, 3H), 3.79 (s, 3H), 3.56 (s, 3H), 2.63-2.58 (m, 1H), 2.30-2.21 (m, 1H), 2.03-1.97 (m, 1H), 1.83-1.76 (m, 4H), 1.33-1.26 (m, 6H). MS (ESI, m/z): 458.2 [M+H].sup.+.

Example 5: Synthesis of (S)N-(11-iodo-1,2,3,10-tetramethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (12) and (S)N-(11-cyano-1,2,3,9-tetramethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (13)

##STR00031##

[0141] Step A: A mixture containing colchicine (6.0 g, 15.0 mmol), water (165 mL), concentrated hydrochloric acid (1.5 mL) and acetic acid (27.3 mL) was stirred at 100 C. for 5 h. The mixture was extracted with ethyl acetate (200 mL3). The pH was adjusted to 56 with saturated sodium bicarbonate solution. The resulting mixture was extracted with ethyl acetate (200 mL3), the combined organic phases were washed with saturated brine (100 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure to afford (S)N-(10-hydroxy-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (9) crude (6.40 g). The compound was used directly in the next step of the reaction without purification.

[0142] Step B: NIS (3.59 g, 16.0 mmol) was added to a solution of crude compound 9 (5.60 g) in acetonitrile (70 mL) and after addition, the resulting mixture was stirred at room temperature overnight. Water (200 mL) was added and the excess NIS was quenched with 2 M sodium thiosulfate solution. The mixture was extracted with ethyl acetate (200 mL3), the combined organic phases were washed with saturated brine (100 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with ethyl acetate:methanol=80:120:1) to afford (S)N-(10-hydroxy-11-iodo-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (10) (4.0 g). The total yield of the two-step reaction of steps A and B was 59.6%.

[0143] Step C: Potassium carbonate (1.40 g, 10.1 mmol) and iodomethane (1.19 g, 8.38 mmol) were added to a solution of compound 10 (3.50 g, 6.85 mmol) in DMF (35 mL), and after addition, the resulting mixture was stirred at room temperature overnight. Water (140 mL) was added and the product was extracted with ethyl acetate (70 mL3). The combined organic phases were washed sequentially with water (40 mL2) and saturated saline (40 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with petroleum ether:ethyl acetate:methanol=1:1:00:30:1) to afford (S)N-(11-iodo-1,2,3,9-tetramethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (11) (1.20 g, ethyl acetate:methanol=5:1, R.sub.f=0.3) and (S)N-(11-iodo-1,2,3,10-tetramethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (12) (270 mg, ethyl acetate:methanol=5:1, R.sub.f=0.5). The yields were 33.3% and 7.50%, respectively. Compound 12: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.61 (d, J=7.2 Hz, 1H), 7.78 (s, 1H), 7.07 (s, 1H), 6.79 (s, 1H), 4.29-4.23 (m, 1H), 3.90 (s, 3H), 3.85 (s, 3H), 3.81 (s, 3H), 3.64 (s, 3H), 2.64-2.59 (m, 1H), 2.29-2.25 (m, 1H), 2.07-2.03 (m, 1H), 1.85-1.79 (m, 4H). MS (ESI, m/z): 526.0 [M+H].sup.+.

[0144] Step D: To a solution of compound 11 (220 mg, 0.419 mmol) in DMF (5 mL) was added cuprous cyanide (100 mg, 1.12 mmol), and after addition, the resulting mixture was stirred at 100 C. for 3 hours. Water (20 mL) was added and the product was extracted with ethyl acetate (20 mL3). The combined organic phases were washed sequentially with water (15 mL2) and saturated saline (10 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with ethyl acetate:methanol=40:110:1) to afford (S)N-(11-cyano-1,2,3,9-tetramethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (13). .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.78 (d, J=7.6 Hz, 1H), 7.86 (s, 1H), 7.19 (s, 1H), 6.86 (s, 1H), 4.40-4.35 (m, 1H), 4.00 (s, 3H), 3.87 (s, 3H), 3.83 (s, 3H), 3.59 (s, 3H), 2.62-2.60 (m, 1H), 2.26-2.10 (m, 3H), 1.91 (s, 3H). MS (ESI, m/z): 425.1 [M+H].sup.+.

Example 6: Synthesis of (S)N-(11-bromo-1,2,3,10-tetramethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (14) and (S)N-(11-bromo-1,2,3,9-tetramethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (15)

##STR00032##

[0145] The experimental operations for the synthesis of compounds 14 and 15 using compound 9 as a raw material were described in Steps B and C of Example 5, wherein NIS in Step B of Example 5 was replaced with NBS. Compound 14: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.61 (d, J=7.6 Hz, 1H), 7.49 (s, 1H), 7.08 (s, 1H), 6.79 (s, 1H), 4.29-4.23 (m, 1H), 3.92 (s, 3H), 3.84 (s, 3H), 3.79 (s, 3H), 3.61 (s, 3H), 2.64-2.59 (m, 1H), 2.30-2.23 (m, 1H), 2.05-1.99 (m, 1H), 1.86-1.80 (m, 4H). MS (ESI, m/z): 478.0 [M+H].sup.+. Compound 15: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.72 (d, J=7.2 Hz, 1H), 8.19 (s, 1H), 7.14 (s, 1H), 6.84 (s, 1H), 4.35-4.30 (m, 1H), 3.94 (s, 3H), 3.85 (s, 3H), 3.80 (s, 3H), 3.59 (s, 3H), 2.59-2.56 (m, 1H), 2.22-2.05 (m, 3H), 1.89 (s, 3H). MS (ESI, m/z): 478.0 [M+H].sup.+.

Example 7: Synthesis of (S)N-(1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (16)

##STR00033##

[0146] To a mixture containing compound 14 (150 mg, 0.314 mmol), methylboronic acid (38 mg, 0.635 mmol), potassium carbonate (216 mg, 1.57 mmol) and toluene (10 mL) was added 1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (23 mg, 0.0314 mmol), after addition, the resulting mixture was stirred at 100 C. under nitrogen overnight. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with ethyl acetate:dichloromethane:methanol=1:1:030:10:1) to afford (S)N-(1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (16). .sup.1H NMR (CDCl.sub.3, 400 MHz) 7.63 (s, 1H), 7.02 (s, 1H), 6.56 (s, 1H), 6.31 (d, J=6.0 Hz, 1H), 4.64-4.59 (m, 1H), 3.95-3.91 (m, 9H), 3.66 (s, 3H), 2.53-2.48 (m, 1H), 2.40-2.27 (m, 5H), 2.06 (s, 3H), 2.00-1.93 (m, 1H). MS (ESI, m/z): 414.1 [M+H].sup.+.

Example 8: Synthesis of (S)N-(1,2,3,9-tetramethoxy-11-methyl-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (17)

##STR00034##

[0147] See Example 7 for experimental operations to synthesize compound 17 using compound 15 as a raw material. .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.56 (d, J=7.6 Hz, 1H), 7.08 (s, 1H), 7.07 (s, 1H), 6.78 (s, 1H), 4.32-4.26 (m, 1H), 3.85-3.80 (m, 9H), 3.58 (s, 3H), 2.63-2.58 (m, 1H), 2.30-2.21 (m, 4H), 2.04-1.98 (m, 1H), 1.85-1.77 (m, 4H). MS (ESI, m/z): 414.1 [M+H].sup.+.

Example 9: Synthesis of N{(S)-1,2,3-trimethoxy-9-oxo-10-{[(S)-tetrahydrofuran-3-yl]oxy}-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (18) and N{(S)-1,2,3-trimethoxy-10-oxo-9-{[(S)-tetrahydrofuran-3-yl]oxy}-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (19)

##STR00035##

[0148] Diisopropyl azodicarboxylate (787 mg, 3.89 mmol) was added to a solution of compound 9 (500 mg, 1.30 mmol), triphenylphosphine (1.02 g, 3.89 mmol) and (R)-3-hydroxytetrahydrofuran (137 mg, 1.56 mmol) in THF (5 mL) under nitrogen. After addition, the resulting mixture was stirred overnight at room temperature. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC and SFC to afford N{(S)-1,2,3-trimethoxy-9-oxo-10-{[(S)-tetrahydrofuran-3-yl]oxy}-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (18) and N{(S)-1,2,3-trimethoxy-10-oxo-9-{[(S)-tetrahydrofuran-3-yl]oxy}-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (19). Compound 18: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.57 (d, J=7.6 Hz, 1H), 7.13 (s, 1H), 7.11-7.03 (m, 2H), 6.77 (s, 1H), 5.15-5.13 (m, 1H), 4.36-4.29 (m, 1H), 3.95-3.91 (m, 1H), 3.87-3.82 (m, 5H), 3.78-3.73 (m, 4H), 3.52 (s, 3H), 2.61-2.57 (m, 1H), 2.33-2.20 (m, 2H), 2.07-1.95 (m, 2H), 1.88-1.86 (m, 4H). MS (ESI, m/z): 456.2 [M+H].sup.+. Compound 19: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.67 (d, J=6.8 Hz, 1H), 7.22 (d, J=12.8 Hz, 1H), 7.02 (s, 1H), 6.94 (d, J=12.8 Hz, 1H), 6.80 (s, 1H), 5.12 (s, 1H), 4.34-4.30 (m, 1H), 3.99-3.95 (m, 1H), 3.91-3.78 (m, 9H), 3.56 (s, 3H), 2.59-2.56 (m, 1H), 2.35-2.26 (m, 1H), 2.21-2.03 (m, 3H), 1.93-1.87 (m, 4H). MS (ESI, m/z): 456.3 [M+H].sup.+.

Example 10: Synthesis of N{(S)-11-iodo-1,2,3-trimethoxy-9-oxo-10-{[(R)-tetrahydrofuran-3-yl]oxy}-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (20), N{(S)-11-iodo-1,2,3-trimethoxy-10-oxo-9-{[(R)-tetrahydrofuran-3-yl]oxy}-5,6,7,10-tetrahydrobenzo[a]heptahydro-7-yl}acetamide (21), N{(S)-11-iodo-1,2,3-trimethoxy-9-oxo-10-{[(S)-tetrahydrofuran-3-yl]oxy}-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (22) and N{(S)-11-iodo-1,2,3-trimethoxy-10-oxo-9-{[(S)-tetrahydrofuran-3-yl]oxy}-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (23)

##STR00036##

[0149] See Example 9 for experimental operations to synthesize compounds 20 and 21, 22 and 23 using compound 10 and (S)-3-hydroxytetrahydrofuran (or (R)-3-hydroxytetrahydrofuran). compound 20: .sup.1H NMR (CDCl.sub.3, 400 MHz) 8.08 (s, 1H), 7.17 (s, 1H), 6.50 (s, 1H), 6.31 (d, J=6.8 Hz, 1H), 5.71-5.68 (m, 1H), 4.60-4.53 (m, 1H), 4.20-4.14 (m, 1H), 4.02-3.83 (m, 9H), 3.73 (s, 3H), 2.55-2.50 (m, 1H), 2.46-2.34 (m, 2H), 2.27-2.14 (m, 2H), 2.00 (s, 3H), 1.79-1.72 (m, 1H). MS (ESI, m/z): 582.2 [M+H].sup.+. compound 21: .sup.1H NMR (CDCl.sub.3, 400 MHz) 8.70 (s, 1H), 7.16 (s, 1H), 6.56 (s, 1H), 6.18 (d, J=6.0 Hz, 1H), 5.35-5.33 (m, 1H), 4.53-4.47 (m, 1H), 4.01-3.89 (m, 10H), 3.72 (s, 3H), 2.54-2.47 (m, 1H), 2.38-2.27 (m, 2H), 2.22-2.13 (m, 2H), 2.04-1.92 (m, 4H). MS (ESI, m/z): 582.2 [M+H].sup.+. compound 22: .sup.1H NMR (CDCl.sub.3, 400 MHz) 8.09 (s, 1H), 7.19 (s, 1H), 6.50 (s, 1H), 6.36 (d, J=6.8 Hz, 1H), 5.64-5.62 (m, 1H), 4.58-4.52 (m, 1H), 4.18-4.08 (m, 3H), 3.97-3.89 (m, 7H), 3.74 (s, 3H), 2.55-2.50 (m, 1H), 2.45-2.37 (m, 1H), 2.27-2.18 (m, 2H), 2.10-2.00 (m, 5H). MS (ESI, m/z): 582.1 [M+H].sup.+. compound 23: .sup.1H NMR (CDCl.sub.3, 400 MHz) 8.70 (s, 1H), 7.20 (s, 1H), 6.56 (s, 1H), 6.31 (s, 1H), 5.25 (s, 1H), 4.51-4.48 (m, 1H), 4.06-3.85 (m, 10H), 3.72 (s, 3H), 2.53-2.51 (m, 1H), 2.38-2.29 (m, 2H), 2.23-2.16 (m, 2H), 2.05-1.96 (m, 4H). MS (ESI, m/z): 582.1 [M+H].sup.+.

Example 11: Synthesis of N{(S)-1,2,3-trimethoxy-9-oxo-10-{[(R)-tetrahydrofuran-3-yl]oxy}-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (24), N{(S)-1,2,3-trimethoxy-10-oxo-9-{[(R)-tetrahydrofuran-3-yl]oxy}-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (25), (S)N-(1,2,3-trimethoxy-9-oxo-10-[(tetrahydrofuran-3-yl)oxy]-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (26) and (S)N-(1,2,3-trimethoxy-10-oxo-9-[(tetrahydrofuran-3-yl)oxy]-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (27)

##STR00037##

[0150] See Example 9 for experimental operations to synthesize compounds 24, 25, 26 and 27 using compound 9 and (S)-3-hydroxytetrahydrofuran (or 3-hydroxytetrahydrofuran) as raw materials. compound 24: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.57 (d, J=7.6 Hz, 1H), 7.13 (s, 1H), 7.10-7.02 (m, 2H), 6.77 (s, 1H), 5.16-5.13 (m, 1H), 4.35-4.30 (m, 1H), 3.95-3.91 (m, 1H), 3.86-3.82 (m, 5H), 3.79-3.75 (m, 4H), 3.53 (s, 3H), 2.61-2.57 (m, 1H), 2.34-2.18 (m, 2H), 2.04-2.00 (m, 2H), 1.92-1.84 (m, 4H). MS (ESI, m/z): 456.4 [M+H].sup.+. compound 25: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.65 (d, J=7.2 Hz, 1H), 7.22 (d, J=12.8 Hz, 1H), 6.96-6.93 (m, 2H), 6.81 (s, 1H), 5.16-5.13 (m, 1H), 4.34-4.29 (m, 1H), 3.99-3.95 (m, 1H), 3.93-3.83 (m, 4H), 3.81-3.74 (m, 5H), 3.56 (s, 3H), 2.58-2.55 (m, 1H), 2.36-2.28 (m, 1H), 2.22-2.02 (m, 4H), 1.88 (s, 3H). MS (ESI, m/z): 456.4 [M+H].sup.+. compound 26: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.58 (d, J=7.2 Hz, 1H), 7.13-7.02 (m, 3H), 6.77 (s, 1H), 5.14 (s, 1H), 4.34-4.29 (m, 1H), 3.96-3.91 (m, 1H), 3.87-3.73 (m, 9H), 3.52 (s, 3H), 2.61-2.57 (m, 1H), 2.33-2.18 (m, 2H), 2.04-1.96 (m, 2H), 1.87-1.79 (m, 4H). MS (ESI, m/z): 456.1 [M+H].sup.+. compound 27: .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.68-8.65 (m, 1H), 7.24-7.21 (m, 1H), 7.02-6.93 (m, 2H), 6.81 (s, 1H), 5.14 (s, 1H), 4.33-4.28 (m, 1H), 3.99-3.95 (m, 1H), 3.91-3.74 (m, 10H), 3.56 (s, 3H), 2.60-2.54 (m, 1H), 2.34-2.30 (m, 1H), 2.19-2.03 (m, 2H), 1.92-1.84 (m, 4H). MS (ESI, m/z): 456.2 [M+H].sup.+.

Example 12: Synthesis of N{(S)-1,2,3-trimethoxy-11-methyl-9-oxo-10-{[(R)-tetrahydrofuran-3-yl]oxy}-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (28), N{(S)-1,2,3-trimethoxy-11-methyl-10-oxo-9-{[(R)-tetrahydrofuran-3-yl]oxy}-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (29), N{(S)-1,2,3-trimethoxy-11-methyl-9-oxo-10-{[(S)-tetrahydrofuran-3-yl]oxy}-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (30) and N{(S)-1,2,3-trimethoxy-11-methyl-10-oxo-9-{[(S)-tetrahydrofuran-3-yl]oxy}-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (31)

##STR00038##

[0151] See Example 7 for experimental operations to synthesize compound 28 (or 29, 30, 31) using compound 20 (or 21, 22, 23) as a raw material, respectively. compound 28: .sup.1H NMR (DMSO-d6, 400 MHz) 8.56 (d, J=7.6 Hz, 1H), 7.09 (d, J=4.4 Hz, 2H), 6.77 (s, 1H), 5.48-5.46 (m, 1H), 4.33-4.27 (m, 1H), 3.93-3.63 (m, 10H), 3.58 (s, 3H), 2.62-2.57 (m, 1H), 2.34-2.20 (m, 4H), 2.15-1.98 (m, 3H), 1.85-1.77 (m, 4H). MS (ESI, m/z): 470.1 [M+H].sup.+. compound 29: .sup.1H NMR (DMSO-d6, 400 MHz) 8.62 (d, J=7.6 Hz, 1H), 7.45 (d, J=0.8 Hz, 1H), 6.98 (s, 1H), 6.79 (s, 1H), 5.13-5.10 (m, 1H), 4.32-4.26 (m, 1H), 3.97-3.74 (m, 10H), 3.57 (s, 3H), 2.56-2.54 (m, 1H), 2.32-2.24 (m, 1H), 2.21 (s, 3H), 2.18-1.97 (m, 4H), 1.87 (s, 3H). MS (ESI, m/z): 470.1 [M+H].sup.+. compound 30: .sup.1H NMR (DMSO-d6, 400 MHz) 8.57 (d, J=7.6 Hz, 1H), 7.09 (d, J=5.6 Hz, 2H), 6.76 (s, 1H), 5.45-5.42 (m, 1H), 4.32-4.26 (m, 1H), 3.90-3.73 (m, 10H), 3.58 (s, 3H), 2.61-2.56 (m, 1H), 2.28-2.20 (m, 4H), 2.07-1.90 (m, 3H), 1.84-1.76 (m, 4H). MS (ESI, m/z): 470.4 [M+H].sup.+. compound 31: .sup.1H NMR (DMSO-d6, 400 MHz) 8.65 (d, J=7.6 Hz, 1H), 7.45 (d, J=1.2 Hz, 1H), 7.05 (s, 1H), 6.80 (s, 1H), 5.12-5.09 (m, 1H), 4.34-4.28 (m, 1H), 3.98-3.95 (m, 1H), 3.91-3.78 (m, 9H), 3.58 (s, 3H), 2.57-2.53 (m, 1H), 2.34-2.27 (m, 1H), 2.22 (s, 3H), 2.18-2.00 (m, 3H), 1.94-1.88 (m, 4H). MS (ESI, m/z): 470.1 [M+H].sup.+.

Example 13: Synthesis of methyl (S)-7-acetamido-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (34) and methyl (S)-7-acetamido-1,2,3-trimethoxy-10-oxo-5,6,7,10 tetrahydrobenzo[a]heptalen-9-carboxylate (35)

##STR00039##

[0152] Step A: A mixture containing phosphorus oxychloride (800 mg, 5.22 mmol), colchicine (2.0 g, 5.01 mmol) and DMF (40 mL) was stirred at room temperature for 4 hours. The reaction was quenched with cold water (100 mL) and then extracted with ethyl acetate (50 mL3). The combined organic phases were washed with saturated saline (30 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with dichloromethane:methanol=30:110:1) to afford (S)N-(10-chloro-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (32) and (S)N-(9-chloro-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (33).

[0153] Step B: To a solution of compound 32 (2.40 g, 5.94 mmol) in methanol (40 mL) was added diisopropylethylamine (768 mg, 5.94 mmol) and 1,1-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (1.0 g, 1.37 mmol), and after addition the resulting mixture was stirred at 80 C. under carbon monoxide (50 psi) at 80 C. with stirring overnight. Methyl (S)-7-acetamido-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (34) was then obtained by preparative HPLC and SFC separation. .sup.1H NMR (DMSO-d6, 400 MHz) 8.61 (d, J=7.2 Hz, 1H), 7.65 (d, J=9.2 Hz, 1H), 7.13 (d, J=9.2 Hz, 1H), 7.09 (s, 1H), 6.80 (s, 1H), 4.29-4.23 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.78 (s, 3H), 3.57 (s, 3H), 2.68-2.61 (m, 1H), 2.29-2.24 (m, 1H), 2.06-1.96 (m, 1H), 1.85-1.76 (m, 4H). MS (ESI, m/z): 428.0 [M+H].sup.+.

[0154] The experimental operations of step C was described in step B. Methyl (S)-7-acetamido-1,2,3-trimethoxy-10-oxo-5,6,7,10 tetrahydrobenzo[a]heptalen-9-carboxylate (35) was obtained. .sup.1H NMR (DMSO-d6, 400 MHz) 8.59 (d, J=7.2 Hz, 1H), 7.65 (s, 1H), 7.24 (d, J=12.8 Hz, 1H), 6.96 (d, J=12.8 Hz, 1H), 6.85 (s, 1H), 4.26-4.19 (m, 1H), 3.86 (s, 3H), 3.80 (s, 3H), 3.79 (s, 3H), 3.61 (s, 3H), 2.63-2.58 (m, 1H), 2.26-2.05 (m, 3H), 1.84 (s, 3H). MS (ESI, m/z): 428.1 [M+H].sup.+.

Example 14: Synthesis of (S)N-{1,2,3-trimethoxy-9-oxo-10-(2,2,2-trifluoroethoxy)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (36) and (S)N-{1,2,3-trimethoxy-10-oxo-9-(2,2,2-trifluoroethoxy)-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (37)

##STR00040##

[0155] A mixture containing compound 9 (500 mg, 1.30 mmol), potassium carbonate (717 mg, 5.19 mmol), acetonitrile (1.5 mL), 2,2,2-trifluoroethyl trifluoromethanesulfonate (602 mg, 2.59 mmol) and DMF (3.5 mL) was reacted for 1.5 h at 150 C. in microwave. Water (20 mL) was added and the resulting mixture was extracted with ethyl acetate (30 mL3). The combined organic phases were washed with saturated saline (20 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was separated by preparative HPLC and SFC to afford (S)N-{1,2,3-trimethoxy-9-oxo-10-(2,2,2-trifluoroethoxy)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (36) (290 mg) and (S)N-{1,2,3-trimethoxy-10-oxo-9-(2,2,2-trifluoroethoxy)-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (37) (46 mg). The yields were 47.7% and 7.57%, respectively. Compound 36: .sup.1H NMR (DMSO-d6, 400 MHz) 8.61 (d, J=7.6 Hz, 1H), 7.19-7.17 (m, 2H), 7.08 (d, J=10.4 Hz, 1H), 6.79 (s, 1H), 4.94-4.78 (m, 2H), 4.36-4.30 (m, 1H), 3.84 (s, 3H), 3.79 (s, 3H), 3.53 (s, 3H), 2.63-2.58 (m, 1H), 2.26-2.17 (m, 1H), 2.07-1.97 (m, 1H), 1.87-1.78 (m, 4H). MS (ESI, m/z): 468.1 [M+H].sup.+. compound 37: .sup.1H NMR (DMSO-d6, 400 MHz) 8.59 (d, J=7.6 Hz, 1H), 7.30 (d, J=12.8 Hz, 1H), 7.21 (s, 1H), 7.03 (d, J=12.8 Hz, 1H), 6.83 (s, 1H), 4.93-4.76 (m, 2H), 4.38-4.31 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 2.60-2.56 (m, 1H), 2.21-2.02 (m, 3H), 1.87 (s, 3H). MS (ESI, m/z): 468.0 [M+H].sup.+.

Example 15: Synthesis of (S)N-(10-cyano-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (38)

##STR00041##

[0156] A solution containing compound 32 (1.0 g, 2.48 mmol), zinc cyanide (880 mg, 7.49 mmol), tetrakis(triphenylphosphine)palladium (572 mg, 0.495 mmol) and DMF (20 mL) was stirred for 1 h at 100 C. under nitrogen. After the mixture was cooled to room temperature, water (100 mL) was added and the mixture was extracted with ethyl acetate (100 mL2). The combined organic phases were washed with saturated saline (50 mL2) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was separated by preparative HPLC and SFC to give (S)N-(10-cyano-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (38). .sup.1H NMR (DMSO-d6, 400 MHz) 8.63 (d, J=7.2 Hz, 1H), 8.07 (d, J=9.6 Hz, 1H), 7.16-7.12 (m, 2H), 6.81 (s, 1H), 4.30-4.23 (m, 1H), 3.82 (s, 3H), 3.79 (s, 3H), 3.59 (s, 3H), 2.67-2.62 (m, 1H), 2.33-2.28 (m, 1H), 2.03-2.00 (m, 1H), 1.84-1.80 (m, 4H). MS (ESI, m/z): 395.4 [M+H].sup.+.

Example 16: Synthesis of (S)N-(9-cyano-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (39)

##STR00042##

[0157] See Example 15 for experimental operations to synthesize compound 39 using compound 33 as a raw material. .sup.1H NMR (DMSO-d6, 400 MHz) 8.57 (d, J=7.2 Hz, 1H), 7.94 (s, 1H), 7.34 (d, J=12.8 Hz, 1H), 7.04 (d, J=12.8 Hz, 1H), 6.87 (s, 1H), 4.28-4.22 (m, 1H), 3.86 (s, 3H), 3.79 (s, 3H), 3.64 (s, 3H), 2.61-2.58 (m, 1H), 2.24-2.08 (m, 3H), 1.87 (s, 3H). MS (ESI, m/z): 395.3 [M+H].sup.+.

Example 17: Synthesis of methyl (S)-7-acetamido-1,2,3,10-tetramethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-11-carboxylate (40)

##STR00043##

[0158] See step B in Example 13 for experimental operations to synthesize compound 40 using compound 12 as a raw material. .sup.1H NMR (DMSO-d6, 400 MHz) 8.62 (d, J=7.6 Hz, 1H), 7.18 (s, 1H), 6.94 (s, 1H), 6.79 (s, 1H), 4.30-4.26 (m, 1H), 3.88 (s, 3H), 3.84 (s, 6H), 3.78 (s, 3H), 3.56 (s, 3H), 2.62-2.59 (m, 1H), 2.26-2.24 (m, 1H), 2.03-2.01 (m, 1H), 1.85-1.77 (m, 4H). MS (ESI, m/z): 458.4 [M+H].sup.+.

Example 18: Synthesis of (S)-1-methyl-(1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)urea (44)

##STR00044##

[0159] Step A: A mixture containing compound 16 (1.35 g, 3.27 mmol), triethylamine (330 mg, 3.27 mmol), DMAP (800 mg, 6.55 mmol), di-tert-butyl dicarbonate (2.30 g, 10.5 mmol) and acetonitrile (13 mL) was stirred for 2 hours at 80 C., and then stirred at reflux overnight after di-tert-butyl dicarbonate (1.40 g, 6.41 mmol) was added. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with petroleum ether:ethyl acetate=2:3) to give tert-butyl (S)-acetyl (1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)carbamate (41) (921 mg). The yield was 54.8%. .sup.1H NMR (DMSO-d6, 400 MHz) 7.18 (s, 1H), 7.06 (s, 1H), 6.78 (s, 1H), 4.91-4.87 (m, 1H), 3.84 (s, 6H), 3.78 (s, 3H), 3.57 (s, 3H), 2.72-2.67 (m, 1H), 2.54-2.47 (m, 1H), 2.36-2.31 (m, 1H), 2.28 (s, 3H), 2.24 (s, 3H), 1.95-1.87 (m, 1H), 1.49 (s, 9H).

[0160] Step B: A solution of compound 41 (1.10 g, 2.14 mmol) and sodium methanolate (463 mg, 8.57 mmol) in methanol (8 mL) was stirred at 40 C. for 1 hour. Saturated ammonium chloride solution (25 mL) was added, the mixture was extracted with ethyl acetate (30 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure to give tert-butyl (S)-(1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)carbamate (42) (900 mg). The yield was 89.2%.

[0161] Step C: A mixture containing compound 42 (900 mg, 1.91 mmol), trifluoroacetic acid (3 mL) and dichloromethane (10 mL) was stirred at room temperature for 4 hours. Water (30 mL) was added and the pH was adjusted to 78 with saturated sodium bicarbonate solution, the mixture was extracted with ethyl acetate (30 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with dichloromethane:methanol=200:3, supplemented by 0.2% triethylamine) to give (S)-7-amino-1,2,3,10-tetramethoxy-11-methyl-6,7-dihydrobenzo[a]heptalen-9(5H)-one (43) (490 mg). The yield was 69.1%.

[0162] Step D: Methylcarbamoyl chloride (102 mg, 1.09 mmol) was added dropwise to a solution of compound 43 (170 mg, 0.458 mmol) and triethylamine (139 mg, 1.38 mmol) in dichloromethane (5 mL) in an ice-water bath, and after addition, the resulting mixture was stirred at room temperature overnight, then heated up to 35 C. and continued stirring for 2 hours. Water (15 mL) was added, and the mixture was extracted with dichloromethane (30 mL2) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with petroleum ether:ethyl acetate=1:12:3) to give (S)-1-methyl-(1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)urea (44). .sup.1H NMR (DMSO-d6, 400 MHz) 7.83 (s, 1H), 7.25 (s, 1H), 6.81 (s, 1H), 6.61 (d, J=8.4 Hz, 1H), 5.77-5.73 (m, 1H), 4.43-4.36 (m, 1H), 3.85 (s, 6H), 3.79 (s, 3H), 3.50 (s, 3H), 2.57-2.53 (m, 7H), 2.21-2.16 (m, 1H), 2.05-1.96 (m, 1H), 1.81-1.73 (m, 1H). MS (ESI, m/z): 429.1 [M+H].sup.+.

Example 19: Synthesis of (S)N-(1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)propanamide (45)

##STR00045##

[0163] The experimental operations for the synthesis of compound 45 were described in Step D of Example 18, wherein methylcarbamoyl chloride was replaced with propionyl chloride in Step D of Example 18. .sup.1H NMR (DMSO-d6, 400 MHz) 8.45 (d, J=8.4 Hz, 1H), 7.83 (s, 1H), 7.29 (s, 1H), 6.84 (s, 1H), 4.56-4.49 (m, 1H), 3.87 (s, 6H), 3.81 (s, 3H), 3.53 (s, 3H), 2.58-2.55 (m, 4H), 2.22-2.15 (m, 3H), 2.09-2.01 (m, 1H), 1.96-1.88 (m, 1H), 1.02 (t, J=7.6 Hz, 3H). MS (ESI, m/z): 428.1 [M+H].sup.+.

Example 20: (S)-7-acetamido-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylic acid (46), (S)-7-acetamido-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylic acid (47), (S)-7-acetamido-1,2,3-trimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalene-10-carboxamide (48) and (S)-7-acetamido-1,2,3-trimethoxy-N-methyl-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxamide (49)

##STR00046## ##STR00047##

[0164] Step A: A mixture containing compound 34 (2.03 g, 2.34 mmol), 6 M hydrochloric acid (8.83 mL) and acetic acid (10 mL) was stirred for 4 hours at room temperature. Water (40 mL) was added, and the mixture was extracted with dichloromethane (20 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)-7-acetamido-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylic acid (46). .sup.1H NMR (DMSO-d6, 400 MHz) 8.68 (d, J=7.2 Hz, 1H), 8.26 (d, J=10.0 Hz, 1H), 8.41 (d, J=10.0 Hz, 1H), 7.38 (s, 1H), 6.83 (s, 1H), 4.36-4.30 (m, 1H), 3.86 (s, 3H), 3.80 (s, 3H), 3.59 (s, 3H), 2.67-2.63 (m, 1H), 2.26-2.23 (m, 1H), 2.06-2.03 (m, 1H), 1.90-1.82 (m, 4H). MS (ESI, m/z): 414.0 [M+H].sup.+.

[0165] Step B: To a mixture containing Compound 46 (1.0 g, 2.42 mmol), diisopropylethylamine (1.56 g, 12.1 mmol) and DMF (10 mL) was added HATU (1.38 g, 3.63 mmol) under an ice-water bath, and after addition, it was stirred for 0.5 h at room temperature. Then methylamine hydrochloride (196 mg, 2.90 mmol) was added and then stirring was continued for 2 hours. Water (40 mL) was added and the product was extracted with ethyl acetate (20 mL3), the combined organic phases were washed with saturated saline (20 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC and SFC to give (S)-7-acetamido-1,2,3-trimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (48). .sup.1H NMR (DMSO-d6, 400 MHz) 9.24 (q, J=4.4 Hz, 1H), 8.63 (d, J=7.2 Hz, 1H), 8.19 (d, J=10.0 Hz, 1H), 7.29 (d, J=10.0 Hz, 1H), 7.23 (s, 1H), 6.83 (s, 1H), 4.33-4.27 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 2.81 (d, J=4.4 Hz, 3H), 2.67-2.62 (m, 1H), 2.29-2.20 (m, 1H), 2.08-2.00 (m, 1H), 1.88-1.78 (m, 4H). MS (ESI, m/z): 427.4 [M+H].sup.+.

[0166] The experimental operations of Steps C and D are described in turn in Steps A and B, wherein Compound 34 in Step A was replaced with Compound 35 to give (S)-7-acetamido-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylic acid (47) and (S)-7-acetamido-1,2,3-trimethoxy-N-methyl-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylic acid (49). Compound 47: .sup.1H NMR (DMSO-d6, 400 MHz) 8.78 (d, J=7.2 Hz, 1H), 8.42 (s, 1H), 7.50 (d, J=12.8 Hz, 1H), 7.26 (d, J=12.8 Hz, 1H), 6.88 (s, 1H), 4.29-4.23 (m, 1H), 3.87 (s, 3H), 3.80 (s, 3H), 3.62 (s, 3H), 2.62-2.58 (m, 1H), 2.26-2.07 (m, 3H), 1.85 (s, 3H). MS (ESI, m/z): 414.0 [M+H].sup.+. Compound 49: .sup.1H NMR (DMSO-d6, 400 MHz) 9.31 (q, J=4.4 Hz, 1H), 8.72 (d, J=6.8 Hz, 1H), 8.42 (s, 1H), 7.28 (d, J=12.8 Hz, 1H), 7.07 (d, J=12.8 Hz, 1H), 6.85 (s, 1H), 4.29-4.22 (m, 1H), 3.86 (s, 3H), 3.80 (s, 3H), 3.60 (s, 3H), 2.82 (d, J=4.4 Hz, 3H), 2.62-2.57 (m, 1H), 2.21-2.06 (m, 3H), 1.84 (s, 3H). MS (ESI, m/z): 427.4 [M+H].sup.+.

Example 21: Synthesis of (S)N-(1,2,3-trimethoxy-10-trideutero-methoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (51)

##STR00048##

[0167] Step A: To a solution of (S)N-(11-bromo-10-hydroxy-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (1.60 g, 3.43 mmol), deuterated methanol (0.5 mL) and triphenylphosphine (2.70 g, 10.3 mmol) in THF (32 mL) was added diisopropyl azodicarboxylate (2.08 g, 10.3 mmol) under an ice-water bath, and after addition, the resulting mixture was stirred under nitrogen at room temperature overnight. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluted with petroleum ether:ethyl acetate=1:10:1) to afford (S)N-(11-bromo-1,2,3-trimethoxy-10-trideutero-methoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (50) (589 mg). The yield was 35.7%.

[0168] The experimental operations of step B were described in Example 7 to afford (S)N-(1,2,3-trimethoxy-10-trideutero-methoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (51). .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.54 (d, J=7.6 Hz, 1H), 7.07 (s, 1H), 7.06 (s, 1H), 6.76 (s, 1H), 4.31-4.25 (m, 1H), 3.83 (s, 3H), 3.79 (s, 3H), 3.57 (s, 3H), 2.61-2.56 (m, 1H), 2.29-2.21 (m, 4H), 2.05-1.95 (m, 1H), 1.84-1.76 (m, 4H). MS (ESI, m/z): 417.2 [M+H].sup.+.

Example 22: Synthesis of (S)N-[1,2,3-trimethoxy-9-oxo-10-(2-oxo-pyrrolidin-1-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (52)

##STR00049##

[0169] Under an ice-water bath, 60% sodium hydride (120 mg, 3.00 mmol) was added in batches to a solution of pyrrolidone (320 mg, 3.76 mmol) in THF (10 mL), then stirring was continued at this temperature for 0.5 h. Colchicine (1.0 g, 2.50 mmol) was then added. After addition, the resulting mixture was stirred at room temperature for 3 hours. Saturated ammonium chloride solution (20 mL) was added to the reaction mixture, which was extracted with ethyl acetate (20 mL3). The combined organic phases were washed with saturated saline (20 mL2) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was isolated and purified by preparative HPLC to give (S)N-[1,2,3-trimethoxy-9-oxo-10-(2-oxo-pyrrolidin-1-yl)5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (52). .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.59 (d, J=7.6 Hz, 1H), 7.56 (d, J=10.0 Hz, 1H), 7.14 (s, 1H), 7.10 (d, J=10.0 Hz, 1H), 6.79 (s, 1H), 4.35-4.29 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.67-3.61 (m, 2H), 3.57 (s, 3H), 2.67-2.61 (m, 1H), 2.42 (t, J=8.0 Hz, 2H), 2.33-2.22 (m, 1H), 2.11-1.99 (m, 3H), 1.88-1.79 (m, 4H). MS (ESI, m/z): 453.4 [M+H].sup.+.

Example 23: Synthesis of methyl (S)-(7-acetamido-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-yl)methylcarbamate (54)

##STR00050##

[0170] Step A: A mixture containing colchicine (5.0 g, 12.5 mmol), ethanol (50 mL) and 30% aqueous methylamine (25 mL) was stirred at 80 C. for 1 hour. The solvent was evaporated under reduced pressure and then the mixture was pulped with petroleum ether to give (S)N-(10-methylamino-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (53) (4.90 g). The yield was 98.2%.

[0171] Step B: 60% sodium hydride (602 mg, 15.1 mmol) was added to a solution of compound 53 (1.0 g, 2.51 mmol) in THF (10 mL) in batches under an ice-water bath and then stirring was continued for 30 min at this temperature. Methyl chloroformate (2.57 g, 27.2 mmol) was then added, and after addition, the resulting mixture was stirred at room temperature for 1 hour. Water (50 mL) was added and the product was extracted with ethyl acetate (50 mL2). The combined organic phases were washed with saturated saline (30 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give methyl (S)-(7-acetamido-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-yl)methylcarbamate (54). .sup.1H NMR (DMSO-d6, 400 MHz) 8.62 (d, J=7.6 Hz, 1H), 7.57 (d, J=10.4 Hz, 1H), 7.17 (s, 1H), 7.05 (d, J=10.4 Hz, 1H), 6.80 (s, 1H), 4.36-4.30 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.59 (s, 3H), 3.57 (s, 3H), 3.07 (s, 3H), 2.66-2.61 (m, 1H), 2.30-2.22 (m, 1H), 2.08-1.99 (m, 1H), 1.90-1.79 (m, 4H). MS (ESI, m/z): 457.4 [M+H].sup.+.

Example 24: Synthesis of (S)-7-acetamido-1,2,3-trimethoxy-N-ethyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (55), (S)-7-acetamido-1,2,3-trimethoxy-N-(trideuterio)methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (56), (S)-7-acetamido-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (57), (S)-7-acetamido-1,2,3-trimethoxy-N-phenyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (58), (S)-7-acetamido-1,2,3-trimethoxy-N,N-dimethyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (59), N{(S)-10-[(S)-2-cyanopyrrolidin-1-carbonyl]-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (60), (S)N-{1,2,3-trimethoxy-9-oxo-10-(pyrrolidin-1-carbonyl)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (61) and (S)N-{1,2,3-trimethoxy-10-(4-methylpiperazine-1-carbonyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a] heptalen-7-yl}acetamide (62)

##STR00051## ##STR00052##

[0172] The experimental procedures for the synthesis of compound 55 (or 56, 57, 58, 59, 60, 61, 62) using compound 46 and ethylamine (or tri-deuteromethylamine, ammonium chloride, aniline, dimethylamine, (S)-pyrrolidine-2-carbonitrile, pyrrolidine, N-methylpiperazine) as raw materials were described in Step B of Example 20. Compound 55: .sup.1H NMR (DMSO-d6, 400 MHz) 9.35 (s, 1H), 8.65 (d, J=6.0 Hz, 1H), 8.18 (d, J=10.0 Hz, 1H), 7.29 (d, J=10.0 Hz, 1H), 7.22 (s, 1H), 6.81 (s, 1H), 4.30-4.28 (m, 1H), 3.84 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 3.51-3.46 (m, 2H), 2.67-2.63 (m, 1H), 2.25-2.24 (m, 1H), 2.03-2.00 (m, 1H), 1.84-1.82 (m, 4H), 1.12-1.10 (m, 3H). MS (ESI, m/z): 441.4 [M+H].sup.+. compound 56: .sup.1H NMR (DMSO-d6, 400 MHz) 9.22 (s, 1H), 8.64 (d, J=7.2 Hz, 1H), 8.19 (d, J=10.0 Hz, 1H), 7.29 (d, J=10.0 Hz, 1H), 7.22 (s, 1H), 6.81 (s, 1H), 4.33-4.26 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 2.67-2.62 (m, 1H), 2.26-2.24 (m, 1H), 2.03-1.98 (m, 1H), 1.84-1.82 (m, 4H). MS (ESI, m/z): 430.4 [M+H].sup.+. compound 57: .sup.1H NMR (DMSO-d6, 400 MHz) 8.67 (d, J=2.4 Hz, 1H), 8.62 (d, J=7.6 Hz, 1H), 8.18 (d, J=10.0 Hz, 1H), 7.72 (d, J=2.4 Hz, 1H), 7.27 (d, J=10.0 Hz, 1H), 7.22 (s, 1H), 6.81 (s, 1H), 4.33-4.26 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 2.65-2.62 (m, 1H), 2.28-2.25 (m, 1H), 2.05-2.01 (m, 1H), 1.85-1.80 (m, 4H). MS (ESI, m/z): 413.0 [M+H].sup.+. compound 58: .sup.1H NMR (DMSO-d6, 400 MHz) 11.31 (s, 1H), 8.67 (d, J=7.6 Hz, 1H), 8.12 (d, J=10.0 Hz, 1H), 7.71-7.69 (m, 2H), 7.38-7.29 (m, 4H), 7.13-7.10 (m, 1H), 6.82 (s, 1H), 4.36-4.29 (m, 1H), 3.86 (s, 3H), 3.80 (s, 3H), 3.60 (s, 3H), 2.69-2.64 (m, 1H), 2.29-2.26 (m, 1H), 2.07-2.04 (m, 1H), 1.86-1.84 (m, 4H). MS (ESI, m/z): 489.2 [M+H].sup.+. compound 59: .sup.1H NMR (DMSO-d6, 400 MHz) 8.60 (d, J=7.6 Hz, 1H), 7.32 (d, J=9.2 Hz, 1H), 7.11-7.08 (m, 2H), 6.79 (s, 1H), 4.33-4.27 (m, 1H), 3.84 (s, 3H), 3.78 (s, 3H), 3.57 (s, 3H), 2.94 (s, 3H), 2.83 (s, 3H), 2.64-2.61 (m, 1H), 2.31-2.29 (m, 1H), 2.02-1.99 (m, 1H), 1.85-1.81 (m, 4H). MS (ESI, m/z): 441.1 [M+H].sup.+. compound 60: .sup.1H NMR (DMSO-d6, 400 MHz) 8.61 (d, J=7.6 Hz, 1H), 7.32 (d, J=9.2 Hz, 1H), 7.11-7.08 (m, 2H), 6.80 (s, 1H), 4.90-4.87 (m, 1H), 4.32-4.27 (m, 1H), 3.84 (s, 3H), 3.79 (s, 3H), 3.59 (s, 3H), 3.35-3.29 (m, 2H), 2.67-2.66 (m, 1H), 2.33-2.21 (m, 3H), 1.98-1.97 (m, 3H), 1.85-1.81 (m, 4H). MS (ESI, m/z): 492.4 [M+H].sup.+. compound 61: .sup.1H NMR (DMSO-d6, 400 MHz) 8.60 (d, J=7.6 Hz, 1H), 7.36 (d, J=9.2 Hz, 1H), 7.13-7.09 (m, 2H), 6.80 (s, 1H), 4.32-4.29 (m, 1H), 3.86 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 3.43-3.38 (m, 2H), 3.23-3.20 (m, 2H), 2.63-2.62 (m, 1H), 2.30-2.28 (m, 1H), 2.05-2.01 (m, 1H), 1.87-1.83 (m, 8H). MS (ESI, m/z): 467.4 [M+H].sup.+. compound 62: .sup.1H NMR (DMSO-d6, 400 MHz) 8.60 (d, J=7.2 Hz, 1H), 7.31 (d, J=9.6 Hz, 1H), 7.10-7.07 (m, 2H), 6.79 (s, 1H), 4.31-4.28 (m, 1H), 3.85 (s, 3H), 3.78 (s, 3H), 3.58 (s, 5H), 3.20 (s, 2H), 2.63-2.62 (m, 1H), 2.33-2.28 (m, 5H), 2.19 (s, 3H), 2.03-1.98 (m, 1H), 1.85-1.81 (m, 4H). MS (ESI, m/z): 496.4 [M+H].sup.+.

Example 25: Synthesis of (S)N-(1,2,3-trimethoxy-11-methyl-10-morpholino-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (63)

##STR00053##

[0173] A solution of compound 51 (137 mg, 0.329 mmol) and morpholine (143 mg, 1.64 mmol) in acetonitrile (5 mL) was stirred at 85 C. for 48 hours. The solvent was evaporated under reduced pressure. The product was purified by column chromatography (200300 mesh silica gel, eluted with dichloromethane:methanol=100:1) to give (S)N-(1,2,3-trimethoxy-11-methyl-10-morpholino-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (63). .sup.1H NMR (DMSO-d6, 400 MHz) 8.50 (d, J=6.0 Hz, 1H), 7.03 (s, 1H), 6.86 (s, 1H), 6.76 (s, 1H), 4.27-4.22 (m, 1H), 3.84 (s, 3H), 3.79 (s, 3H), 3.68-3.67 (m, 4H), 3.58 (s, 3H), 3.13-3.09 (m, 2H), 3.00-2.96 (m, 2H), 2.62-2.58 (m, 1H), 2.39 (s, 3H), 2.30-2.23 (m, 1H), 2.02-1.97 (m, 1H), 1.86-1.75 (m, 4H). MS (ESI, m/z): 469.2 [M+H].sup.+.

Example 26: Synthesis of (S)N-(9-hydroxymethyl-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptadien-7-yl)acetamide (66)

##STR00054##

[0174] The experimental operations of Step A were described in Step A of Example 13, wherein phosphorus oxychloride in Step A of Example 13 was replaced with phosphorus oxybromide to give Compounds 64 and 65.

[0175] Step B: A mixture containing compound 64 (1.0 g, 2.23 mmol), THF (10 mL) and tetrakis(triphenylphosphine)palladium (258 mg, 0.223 mmol) was stirred under nitrogen at room temperature for 30 minutes, and then tributyltin methanol (716.22 mg, 2.23 mmol) was added. After addition, the resulting mixture was stirred at 65 C. overnight. It was cooled to room temperature and filtered to remove insolubles. The solvent was evaporated under reduced pressure and the product was separated by preparative HPLC and SFC to give (S)N-(9-hydroxymethyl-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptadien-7-yl)acetamide (66). .sup.1H NMR (DMSO-d6, 400 MHz) 8.66 (d, J=6.8 Hz, 1H), 7.84 (s, 1H), 7.21 (d, J=12.8 Hz, 1H), 6.84-6.81 (m, 2H), 5.41-5.38 (m, 1H), 4.50-3.36 (m, 2H), 4.25-4.24 (m, 1H), 3.85 (s, 3H), 3.78 (s, 3H), 3.57 (s, 3H), 2.59-2.56 (m, 1H), 2.17-2.04 (m, 3H), 1.84 (s, 3H). MS (ESI, m/z): 400.0 [M+H].sup.+.

Example 27: Synthesis of (S)N-(10-formyl-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptadien-7-yl)acetamide (68)

##STR00055##

[0176] The experimental operations of Step A were described in Step B of Example 26, wherein Compound 64 in Step B of Example 26 was replaced with Compound 65 to afford (S)N-(10-hydroxymethyl-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptadien-7-yl)acetamide (67). .sup.1H NMR (DMSO-d6, 400 MHz) 8.58 (d, J=7.2 Hz, 1H), 7.58 (d, J=9.6 Hz, 1H), 7.19 (d, J=9.6 Hz, 1H), 7.02 (s, 1H), 6.78 (s, 1H), 5.36 (t, J=5.6 Hz, 1H), 4.39-4.33 (m, 2H), 4.31-4.28 (m, 1H), 3.84 (s, 3H), 3.79 (s, 3H), 3.54 (s, 3H), 2.62-2.56 (m, 1H), 2.27-2.24 (m, 1H), 2.02-1.99 (m, 1H), 1.84-1.79 (m, 4H). MS (ESI, m/z): 400.0 [M+H].sup.+.

[0177] Step B: A mixture containing Compound 67 (100 mg, 0.250 mmol), Dess-Martin oxidant (127 mg, 0.30 mmol) and dichloromethane (10 mL) was stirred at room temperature for 2 hours. The insolubles was removed by filtration. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to afford (S)N-(10-formyl-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptadien-7-yl)acetamide (68). .sup.1H NMR (DMSO-d6, 400 MHz) 10.12 (s, 1H), 8.66 (d, J=7.2 Hz, 1H), 7.82 (d, J=9.2 Hz, 1H), 7.28-7.24 (m, 2H), 6.81 (s, 1H), 4.31-4.25 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.59 (s, 3H), 2.67-2.63 (m, 1H), 2.29-2.27 (m, 1H), 2.03-2.00 (m, 1H), 1.85-1.80 (m, 4H). MS (ESI, m/z): 398.3 [M+H].sup.+.

Example 28: Synthesis of (S)N-[1,2,3-trimethoxy-9-oxo-10-aminosulfonyl-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (69)

##STR00056##

[0178] A solution of compound 32 (300 mg, 0.743 mmol) and sodium 3-methoxy-3-oxopropanesulfinate (155 mg, 0.891 mmol) in DMSO (6 mL) was stirred at room temperature for 2 h. Sodium methanol (40 mg, 0.740 mmol) was then added, and stirring was continued for 15 min at this temperature. Then a solution of hydroxylamine sulfonic acid (392 mg, 3.46 mmol) and sodium acetate (227 mg, 2.77 mmol) in water (1 mL) was added sequentially. After addition, the resulting mixture was stirred overnight at room temperature. Water (30 mL) was added and the product was extracted with ethyl acetate (50 mL2). The combined organic phases were washed with saturated saline (20 mL2) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)N-[1,2,3-trimethoxy-9-oxo-10-aminosulfonyl-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (69). .sup.1H NMR (DMSO-d6, 400 MHz) 8.65 (d, J=7.2 Hz, 1H), 8.19 (d, J=10.0 Hz, 1H), 7.30 (d, J=10.0 Hz, 1H), 7.20 (s, 1H), 7.09 (s, 2H), 6.82 (s, 1H), 4.30-4.27 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.59 (s, 3H), 2.67-2.52 (m, 1H), 2.33-2.27 (m, 1H), 2.06-1.99 (m, 1H), 1.85-1.83 (m, 4H). MS (ESI, m/z): 449.4 [M+H].sup.+.

Example 29: Synthesis of (S)N-[1,2,3-trimethoxy-10-morpholinyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]propanamide (74)

##STR00057## ##STR00058##

[0179] The experimental procedures for the synthesis of compound 74 using colchicine as a raw material were described in turn in Steps A and B of Example 18, Example 25, Step C of Example 18, and Step B of Example 20, wherein Compound 46 in Step B of Example 20 was substituted with propionic acid to give (S)N-[1,2,3-trimethoxy-10-morpholinyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]propanamide (74). .sup.1H NMR (DMSO-d.sub.6, 400 MHz) 8.52 (d, J=7.6 Hz, 1H), 7.13 (d, J=10.8 Hz, 1H), 7.02 (s, 1H), 6.91 (d, J=10.8 Hz, 1H), 6.81 (s, 1H), 4.41-4.37 (m, 1H), 3.89 (s, 3H), 3.84 (s, 3H), 3.80-3.77 (m, 4H), 3.58 (s, 3H), 3.47-3.41 (m, 2H), 3.36-3.33 (m, 2H), 2.59-2.57 (m, 1H), 2.21-2.18 (m, 3H), 2.16-2.05 (m, 1H), 1.95-1.90 (m, 1H), 1.01 (t, J=7.2 Hz, 3H). MS (ESI, m/z): 469.4 [M+H].sup.+.

Example 30: Synthesis of methyl (S)-7-(tert-butoxycarbonyl)amino-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (77), methyl (S)-1,2,3-trimethoxy-7-(3-methylureido)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (79) and (S)-1,2,3-trimethoxy-N-methyl-7-(3-methylureido)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (81)

##STR00059## ##STR00060## ##STR00061##

[0180] Experimental operations for the synthesis of compounds 77, 78, 79, and 81 using compound 71 as a raw material were described in turn in steps A and B of Example 13, steps C and D of Example 18, and Example 20. compound 77: .sup.1H NMR (DMSO-d6, 400 MHz) 7.75 (d, J=7.6 Hz, 1H), 7.64 (d, J=9.6 Hz, 1H), 7.17 (s, 1H), 7.11 (d, J=9.6 Hz, 1H), 6.79 (s, 1H), 4.06-4.00 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.78 (s, 3H), 3.60 (s, 3H), 2.63-2.58 (m, 1H), 2.25-2.20 (m, 1H), 2.01-1.98 (m, 1H), 1.81-1.79 (m, 1H), 1.33 (s, 9H). MS (ESI, m/z): 486.3 [M+H].sup.+. compound 78: MS (ESI, m/z): 386.0 [M+H].sup.+. compound 79: .sup.1H NMR (DMSO-d6, 400 MHz) 7.62 (d, J=9.6 Hz, 1H), 7.15 (s, 1H), 7.11 (d, J=9.6 Hz, 1H), 6.80-6.78 (m, 2H), 5.79 (q, J=4.4 Hz, 1H), 4.19-4.12 (m, 1H), 3.84 (s, 3H), 3.79 (s, 3H), 3.78 (s, 3H), 3.56 (s, 3H), 2.64-2.59 (m, 1H), 2.47 (d, J=4.4 Hz, 3H), 2.26-2.24 (m, 1H), 2.03-2.00 (m, 1H), 1.74-1.71 (m, 1H). MS (ESI, m/z): 443.1 [M+H].sup.+. compound 81: .sup.1H NMR (DMSO-d6, 400 MHz) 9.30 (q, J=4.4 Hz, 1H), 8.20 (d, J=10.0 Hz, 1H), 7.30-7.27 (m, 2H), 6.82-6.80 (m, 2H), 5.80 (q, J=4.4 Hz, 1H), 4.23-4.16 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.57 (s, 3H), 2.81 (d, J=4.4 Hz, 3H), 2.63-2.60 (m, 1H), 2.47 (d, J=4.4 Hz, 3H), 2.23-2.21 (m, 1H), 2.05-2.04 (m, 1H), 1.74-1.73 (m, 1H). MS (ESI, m/z): 442.4 [M+H].sup.+.

Example 31: Synthesis of methyl (S)-1,2,3-trimethoxy-7-(3-methylureido)-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylate (82)

##STR00062##

[0181] The experimental operations for the synthesis of compound 82, using compound 76 as a raw material, were described in turn in step B of Example 13 and steps C and D of Example 18. .sup.1H NMR (DMSO-d6, 400 MHz) 7.71 (s, 1H), 7.23 (d, J=12.8 Hz, 1H), 6.94 (d, J=12.8 Hz, 1H), 6.84 (s, 1H), 6.74 (d, J=6.8 Hz, 1H), 5.80 (q, J=4.4 Hz, 1H), 4.17-4.10 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.77 (s, 3H), 3.60 (s, 3H), 2.58-2.55 (m, 1H), 2.47 (q, J=4.4 Hz, 3H), 2.21-2.16 (m, 2H), 2.14-1.99 (m, 1H). MS (ESI, m/z): 443.1 [M+H].sup.+.

Example 32: Synthesis of (S)N-{1,2,3-trimethoxy-10-(morpholine-4-carbonyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (83) and (S)N-{1,2,3-trimethoxy-9-(morpholine-4-carbonyl)-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (84)

##STR00063##

[0182] See Step B in Example 20 for experimental operations to synthesize compounds 83 or 84 using compound 46 (or 47) and morpholine as raw materials. compound 83: .sup.1H NMR (DMSO-d6, 400 MHz) 8.60 (d, J=7.6 Hz, 1H), 7.35 (d, J=9.6 Hz, 1H), 7.11-7.08 (m, 2H), 6.79 (s, 1H), 4.31-4.28 (m, 1H), 3.85 (s, 3H), 3.78 (s, 3H), 3.63-3.62 (m, 2H), 3.57-3.54 (m, 7H), 3.24-3.22 (m, 2H), 2.68-2.66 (m, 1H), 2.33-2.32 (m, 1H), 2.01-1.98 (m, 1H), 1.85-1.80 (m, 4H). MS (ESI, m/z): 483.4 [M+H].sup.+. Compound 84: .sup.1H NMR (DMSO-d6, 400 MHz) 8.54 (d, J=7.2 Hz, 1H), 7.33 (s, 1H), 7.25 (d, J=12.8 Hz, 1H), 6.94 (d, J=12.8 Hz, 1H), 6.84 (s, 1H), 4.26-4.23 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.66-3.52 (m, 9H), 3.19 (s, 2H), 2.63-2.58 (m, 1H), 2.26-2.23 (m, 1H), 2.21-2.14 (m, 1H), 2.03-2.01 (m, 1H), 1.83 (s, 3H). MS (ESI, m/z): 483.2 [M+H].sup.+.

Example 33: Synthesis of (S)N-{1,2,3-trimethoxy-11-methyl-9-oxo-10-(2,2,2-trifluoroethoxy)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (87) and (S)N-{1,2,3-trimethoxy-11-methyl-10-oxo-9-(2,2,2-trifluoroethoxy)-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl}acetamide (88)

##STR00064##

[0183] The experimental operations for the synthesis of compounds 87 and 88 using compound 10 as a raw material were described in turn in Example 7 and Example 14, wherein toluene in Example 7 was replaced with a solvent mixture of dioxane and water. Compound 87: .sup.1H NMR (DMSO-d6, 400 MHz) 8.58 (d, J=7.2 Hz, 1H), 7.15 (s, 1H), 7.11 (s, 1H), 6.78 (s, 1H), 4.89-4.84 (m, 1H), 4.75-4.70 (m, 1H), 4.31-4.28 (m, 1H), 3.84 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 2.63-2.58 (m, 1H), 2.33 (s, 3H), 2.24-2.22 (m, 1H), 2.03-1.99 (m, 1H), 1.84-1.80 (m, 4H). MS (ESI, m/z): 482.4 [M+H].sup.+. compound 88: .sup.1H NMR (DMSO-d6, 400 MHz) 8.57 (d, J=7.2 Hz, 1H), 7.52 (s, 1H), 7.27 (s, 1H), 6.81 (s, 1H), 4.83-4.76 (m, 2H), 4.34-4.31 (m, 1H), 3.85 (s, 3H), 3.80 (s, 3H), 3.59 (s, 3H), 2.57-2.55 (m, 1H), 2.25 (s, 3H), 2.16-1.98 (m, 3H), 1.86 (s, 3H). MS (ESI, m/z): 482.4 [M+H].sup.+.

Example 34: Synthesis of methyl (S)-7-(ethylsulfonamido)-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (89)

##STR00065##

[0184] The experimental procedures for the synthesis of compound 89 using compound 78 and ethylsulfonyl chloride as raw material were described in step D of Example 18. .sup.1H NMR (DMSO-d6, 400 MHz) 8.08 (d, J=7.6 Hz, 1H), 7.66 (d, J=9.2 Hz, 1H), 7.40 (s, 1H), 7.12 (d, J=9.2 Hz, 1H), 6.80 (s, 1H), 3.95-3.91 (m, 1H), 3.85 (s, 3H), 3.79 (s, 6H), 3.52 (s, 3H), 2.89-2.80 (m, 2H), 2.65-2.60 (m, 1H), 2.28-2.20 (m, 1H), 2.12-2.07 (m, 1H), 1.86-1.79 (m, 1H), 1.09 (t, J=7.2 Hz, 3H). MS (ESI, m/z): 478.4 [M+H].sup.+.

Example 35: Synthesis of methyl (S)-7-(tert-butoxycarbonyl)amino-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylate (90) and methyl (S)-7-(ethylsulfonamido)-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylate (92)

##STR00066##

[0185] Experimental operations for the synthesis of compounds 90 and 92 using compound 76 as a raw material were described in turn in step B of Example 13 and steps C and D of Example 18. compound 90: .sup.1H NMR (DMSO-d6, 400 MHz) 7.71-7.69 (m, 2H), 7.22 (d, J=12.8 Hz, 1H), 6.95 (d, J=12.8 Hz, 1H), 6.84 (s, 1H), 4.01-3.94 (m, 1H), 3.86 (s, 3H), 3.79 (s, 3H), 3.78 (s, 3H), 3.65 (s, 3H), 2.57-2.55 (m, 1H), 2.21-2.03 (m, 3H), 1.33 (s, 9H). MS (ESI, m/z): 486.3 [M+H].sup.+. compound 92: .sup.1H NMR (DMSO-d6, 400 MHz) 8.10 (d, J=7.6 Hz, 1H), 7.96 (s, 1H), 7.23 (d, J=12.8 Hz, 1H), 6.97 (d, J=12.8 Hz, 1H), 6.86 (s, 1H), 3.97-3.91 (m, 1H), 3.86 (s, 3H), 3.81 (s, 3H), 3.79 (s, 3H), 3.57 (s, 3H), 2.87-2.73 (m, 2H), 2.61-2.57 (m, 1H), 2.25-2.07 (m, 3H), 1.11 (t, J=7.2 Hz, 3H). MS (ESI, m/z): 478.3 [M+H].sup.+.

Example 36: Synthesis of methyl (S)-7-(3,3-dimethylureido)-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (93) and methyl (S)-7-(3,3-dimethylureido)-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptahydrogen-9-carboxylate (94)

##STR00067##

[0186] See Step D in Example 18 for experimental operations to synthesize compounds 93 or 94 using compound 78 (or 91) and dimethylcarbamoyl chloride as raw materials. compound 93: .sup.1H NMR (DMSO-d6, 400 MHz) 7.63 (d, J=9.6 Hz, 1H), 7.19 (s, 1H), 7.12 (d, J=9.6 Hz, 1H), 6.86 (d, J=7.2 Hz, 1H), 6.80 (s, 1H), 4.24-4.18 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.78 (s, 3H), 3.57 (s, 3H), 2.79 (s, 6H), 2.65-2.61 (m, 1H), 2.27-2.23 (m, 1H), 2.00-1.95 (m, 2H). MS (ESI, m/z): 457.4 [M+H].sup.+. compound 94: .sup.1H NMR (DMSO-d6, 400 MHz) 7.81 (s, 1H), 7.22 (d, J=12.4 Hz, 1H), 6.93 (d, J=12.4 Hz, 1H), 6.84-6.83 (m, 2H), 4.26-4.19 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.78 (s, 3H), 3.59 (s, 3H), 2.78 (s, 6H), 2.58-2.56 (m, 1H), 2.26-2.10 (m, 3H). MS (ESI, m/z): 457.4 [M+H].sup.+.

Example 37: Synthesis of (S)N-{1,2,3-trimethoxy-9-oxo-10-(1H-tetrazol-1-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (95)

##STR00068##

[0187] A mixture containing compound 1 (200 mg, 0.520 mmol), sodium azide (40.6 mg, 0.624 mmol), triethyl orthoformate (355 mg, 2.39 mmol) and acetic acid (5 mL) was stirred at 90 C. for 2 h. The mixture was extracted with dichloromethane (30 mL2). After the mixture was cooled to room temperature, water (30 mL) was added and the pH was adjusted to 78 with saturated sodium bicarbonate solution. The product was extracted with dichloromethane (30 mL2), and the combined organic phases were washed with saturated brine (10 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)N-{1,2,3-trimethoxy-9-oxo-10-(1H-tetrazol-1-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (95). .sup.1H NMR (DMSO-d6, 400 MHz) 9.86 (s, 1H), 8.70 (d, J=7.2 Hz, 1H), 7.25 (d, J=10.0 Hz, 1H), 7.40 (s, 1H), 7.32 (d, J=10.0 Hz, 1H), 6.84 (s, 1H), 4.39-4.35 (m, 1H), 3.86 (s, 3H), 3.81 (s, 3H), 3.61 (s, 3H), 2.70-2.66 (m, 1H), 2.35-2.32 (m, 1H), 2.30-2.05 (m, 1H), 1.92-1.86 (m, 4H). MS (ESI, m/z): 438.3 [M+H].sup.+.

Example 38: Synthesis of (S)N-{1,2,3-trimethoxy-9-oxo-10-(1H-1,2,4-triazol-1-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (96)

##STR00069##

[0188] A mixture containing compound 32 (100 mg, 0.248 mmol), potassium carbonate (103 mg, 0.743 mmol), 1,2,4-triazole (34.2 mg, 0.495 mmol), cuprous iodide (12.7 mg, 0.0669 mmol) and DMSO (2 mL) was reacted for 30 min at 80 C. in microwave. After cooling to room temperature, water (15 mL) was added and the mixture was extracted with dichloromethane (30 mL2). The combined organic phases were washed with saturated saline (10 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)N-{1,2,3-trimethoxy-9-oxo-10-(1H-1,2,4-triazol-1-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (96). .sup.1H NMR (DMSO-d6, 400 MHz) 9.33 (s, 1H), 8.68 (d, J=7.2 Hz, 1H), 8.27 (s, 1H), 8.17 (d, J=10.4 Hz, 1H), 7.40 (s, 1H), 7.34 (d, J=10.4 Hz, 1H), 6.83 (s, 1H), 4.39-4.33 (m, 1H), 3.86 (s, 3H), 3.80 (s, 3H), 3.59 (s, 3H), 2.68-2.63 (m, 1H), 2.32-2.29 (m, 1H), 2.08-2.06 (m, 1H), 2.05-1.86 (m, 4H). MS (ESI, m/z): 437.2 [M+H].sup.+.

Example 39: Synthesis of (S)N-{10-(1H-imidazol-1-yl)-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (97) and (S)N-{1,2,3-trimethoxy-10-(3-methyl-1H-pyrazol-1-yl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (98)

##STR00070##

[0189] See Example 38 for experimental operations to synthesize compounds 97 or 98 using compound 32 and imidazole (or 3-methyl-1H-pyrazole) as raw materials. compound 97: .sup.1H NMR (DMSO-d6, 400 MHz) 8.65 (d, J=6.8 Hz, 1H), 8.12 (s, 1H), 7.72 (dd, J=2.0, 10.0 Hz, 1H), 7.56 (s, 1H), 7.31 (s, 1H), 7.17 (dd, J=2.0, 10.0 Hz, 1H), 7.05 (s, 1H), 6.82 (s, 1H), 4.38-4.32 (m, 1H), 3.85 (s, 3H), 3.80 (s, 3H), 3.59 (s, 3H), 2.68-2.63 (m, 1H), 2.32-2.29 (m, 1H), 2.07-2.04 (m, 1H), 1.87-1.85 (m, 4H). MS (ESI, m/z): 436.2 [M+H].sup.+. Compound 98: .sup.1H NMR (DMSO-d6, 400 MHz) 8.65-8.63 (m, 2H), 8.20 (d, J=10.8 Hz, 1H), 7.34 (s, 1H), 7.30 (d, J=10.8 Hz, 1H), 6.81 (s, 1H), 6.34 (d, J=2.4 Hz, 1H), 4.38-4.32 (m, 1H), 3.85 (s, 3H), 3.80 (s, 3H), 3.58 (s, 3H), 2.66-2.61 (m, 1H), 2.29-2.25 (m, 4H), 2.07-2.04 (m, 1H), 1.90-1.86 (m, 4H). MS (ESI, m/z): 450.2 [M+H].sup.+.

Example 40: Synthesis of (S)N-(1,2,3-trimethoxy-9-oxo-10-(1H-1,2,3-triazol-1-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (99) and (S)N-(1,2,3-trimethoxy-9-oxo-10-(2H-1,2,3-triazol-2-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (100)

##STR00071##

[0190] Experimental operations for the synthesis of compounds 99 and 100 were described in Example 38, wherein 1,2,4-triazole in Example 38 was replaced with 1,2,3-triazole. Compound 99: .sup.1H NMR (DMSO-d6, 400 MHz) 8.67 (d, J=7.2 Hz, 1H), 8.64 (s, 1H), 7.08 (d, J=10.0 Hz, 1H), 7.92 (s, 1H), 7.38 (s, 1H), 7.30 (d, J=10.0 Hz, 1H), 6.83 (s, 1H), 4.40-4.33 (m, 1H), 3.86 (s, 3H), 3.81 (s, 3H), 3.61 (s, 3H), 2.70-2.65 (m, 1H), 2.35-2.32 (m, 1H), 2.08-2.05 (m, 1H), 1.89-1.86 (m, 4H). MS (ESI, m/z): 437.2 [M+H].sup.+. Compound 100: .sup.1H NMR (DMSO-d6, 400 MHz) 8.64 (d, J=7.2 Hz, 1H), 8.08 (s, 2H), 7.83 (d, J=10.0 Hz, 1H), 7.29 (s, 1H), 7.20 (d, J=10.0 Hz, 1H), 6.82 (s, 1H), 4.36-4.30 (m, 1H), 3.86 (s, 3H), 3.80 (s, 3H), 3.61 (s, 3H), 2.69-2.64 (m, 1H), 2.38-2.33 (m, 1H), 2.07-2.03 (m, 1H), 1.90-1.86 (m, 4H). MS (ESI, m/z): 437.1 [M+H].sup.+.

Example 41: Synthesis of methyl (S)-7-acetamido-1,2,3-trimethoxy-10-morpholino-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-11-carboxylate (101)

##STR00072##

[0191] See Example 25 for experimental operations to synthesize compound 101 using compound 40 as a raw material. .sup.1H NMR (DMSO-d6, 400 MHz) 8.48 (d, J=8.4 Hz, 1H), 7.89 (s, 1H), 7.26 (s, 1H), 6.84 (s, 1H), 4.61-4.56 (m, 1H), 3.85 (s, 3H), 3.83 (s, 3H), 3.80 (s, 3H), 3.71-3.51 (m, 9H), 3.10 (s, 2H), 2.58-2.55 (m, 1H), 2.24-2.21 (m, 1H), 2.08-2.04 (m, 1H), 1.91-1.88 (m, 4H). MS (ESI, m/z): 513.4 [M+H].sup.+.

Example 42: Synthesis of (S)-7-(3-cyclopropylcarbonylamino)-1,2,3-trimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (102) and (S)-7-(3-cyclopropylcarbonylamino)-1,2,3-trimethoxy-N-methyl-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxamide (103)

##STR00073##

[0192] Compound 102 or 103 was obtained by synthesizing the corresponding amide using compound 78 (or 91) and cyclopropanecarboxylic acid as raw materials, followed by a condensation reaction of the acid obtained by hydrolysis of the methyl ester with the methylamine, as described in the experimental procedures of Example 20. Compound 102: .sup.1H NMR (DMSO-d6, 400 MHz) 9.26 (q, J=4.8 Hz, 1H), 8.85 (d, J=7.6 Hz, 1H), 8.19 (d, J=9.6 Hz, 1H), 7.30-7.26 (m, 2H), 6.81 (s, 1H), 4.36-4.29 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.55 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.67-2.63 (m, 1H), 2.27-2.25 (m, 1H), 2.08-2.06 (m, 1H), 1.88-1.86 (m, 1H), 1.66-1.62 (m, 1H), 0.67-0.57 (m, 4H). MS (ESI, m/z): 453.3 [M+H].sup.+. compound 103: .sup.1H NMR (DMSO-d6, 400 MHz) 9.27 (q, J=4.4 Hz, 1H), 8.92 (d, J=6.8 Hz, 1H), 8.42 (s, 1H), 7.28 (d, J=12.8 Hz, 1H), 7.07 (d, J=12.8 Hz, 1H), 6.85 (s, 1H), 4.32-4.26 (m, 1H), 3.86 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 2.82 (d, J=4.8 Hz, 3H), 2.62-2.60 (m, 1H), 2.20-2.09 (m, 3H), 1.66-1.63 (m, 1H), 0.65-0.57 (m, 4H). MS (ESI, m/z): 453.3 [M+H].sup.+.

Example 43: Synthesis of (S)N-(8-bromo-1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (105) and (S)N-(8-bromo-1,2,3,9-tetramethoxy-11-methyl-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (106)

##STR00074##

[0193] Step A: To a solution of a mixture of compounds 85 and 86 (1.0 g, 2.50 mmol) in DMF (10 mL) were added lithium bromide (220 mg, 2.53 mmol) and NBS (530 mg, 2.98 mmol), and after addition, the resulting mixture was stirred at room temperature overnight. Water (40 mL) was added and the product was extracted with ethyl acetate (30 mL3), the combined organic phases were washed with saturated saline (20 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure to afford (S)N-(8-bromo-9-hydroxy-1,2,3-trimethoxy-11-methyl-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (104) as crude (3.60 g) (containing some DMF). The compound was used directly in the next step of the reaction without purification.

[0194] The experimental operations of Step B were described in Step C in Example 5, giving (S)N-(1,2,3,10-tetramethoxy-11-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl)acetamide (105) and (S)N-(1,2,3,9-tetramethoxy-11-methyl-9-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-7-yl)acetamide (106). MS (ESI, m/z) of compound 105: 492.0 [M+H].sup.+. MS (ESI, m/z) of compound 106: 492.0 [M+H].sup.+.

Example 44: Synthesis of methyl (S)-1,2,3-trimethoxy-9-oxo-7-(2-oxopiperidin-1-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (108)

##STR00075##

[0195] Step A: See Step B in Example 20 for experimental operations to synthesize Compound 107 using Compound 78 and 5-chlorovaleric acid as raw materials.

[0196] Step B: A mixture containing compound 107 (300 mg, 0.595 mmol), cesium carbonate (582 mg, 1.79 mmol), cuprous iodide (34.0 mg, 0.179 mmol) and DMSO (4 mL) was reacted at 80 C. for 0.5 hours in microwave. After cooling to room temperature, water (20 mL) was added and the product was extracted with ethyl acetate (20 mL3), the combined organic phases were washed with saturated brine (20 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was isolated and purified by preparative HPLC and SFC to give methyl (S)-1,2,3-trimethoxy-9-oxo-7-(2-oxopiperidin-1-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (108). .sup.1H NMR (DMSO-d6, 400 MHz) 7.62 (d, J=9.2 Hz, 1H), 7.12 (d, J=9.2 Hz, 1H), 6.81 (s, 1H), 6.77 (s, 1H), 4.67-4.63 (m, 1H), 3.84 (s, 3H), 3.77 (s, 6H), 3.67-3.64 (m, 1H), 3.59 (s, 3H), 3.45-3.41 (m, 1H), 2.76-2.71 (m, 1H), 2.35-2.19 (m, 4H), 2.05-1.99 (m, 1H), 1.91-1.87 (m, 1H), 1.78-1.74 (m, 2H), 1.63-1.59 (m, 1H). MS (ESI, m/z): 468.4 [M+H].sup.+.

Example 45: Synthesis of methyl (S)-1,2,3-trimethoxy-10-oxo-7-(2-oxopiperidin-1-yl)-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylate (109)

##STR00076##

[0197] See Step B in Example 20 and Step B in Example 44 for experimental operations to synthesize Compound 109 using Compound 91 and 5-chlorovaleric acid as raw materials. .sup.1H NMR (DMSO-d6, 400 MHz) 7.43 (s, 1H), 7.24 (d, J=12.4 Hz, 1H), 6.92 (d, J=12.4 Hz, 1H), 6.86 (s, 1H), 4.65-4.60 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.78 (s, 3H), 3.69-3.66 (m, 1H), 3.60 (s, 3H), 3.39-3.35 (m, 1H), 2.69-2.66 (m, 1H), 2.46-2.42 (m, 1H), 2.27-2.16 (m, 4H), 1.88-1.62 (m, 4H). MS (ESI, m/z): 468.4 [M+H].sup.+.

Example 46: Synthesis of methyl (S)-1,2,3-trimethoxy-9-oxo-7-(3-oxomorpholinyl)-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (110)

##STR00077##

[0198] See Step B in Example 20 and Step B in Example 43 for experimental operations to synthesize Compound 110 using Compound 78 and 2-(2-chloroethoxy)acetic acid as raw materials. .sup.1H NMR (DMSO-d6, 400 MHz) 7.64 (d, J=9.2 Hz, 1H), 7.14 (d, J=9.2 Hz, 1H), 6.83 (s, 1H), 6.82 (s, 1H), 4.62-4.60 (m, 1H), 4.11-3.93 (m, 4H), 3.85 (s, 3H), 3.78-3.71 (m, 7H), 3.59-3.56 (m, 4H), 2.76-2.73 (m, 1H), 2.33-2.31 (m, 1H), 2.23-2.21 (m, 1H), 2.10-2.08 (m, 1H). MS (ESI, m/z): 470.1 [M+H].sup.+.

Example 47: Synthesis of methyl (S)-1,2,3-trimethoxy-7-(N-methylacetamido)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (111)

##STR00078##

[0199] 60% sodium hydride (93.6 mg, 2.34 mmol) was added to a solution of compound 34 (500 mg, 1.17 mmol) in DMF (7 mL) in an ice-water bath, and stirring was continued at this temperature for 0.5 h before iodomethane (664 mg, 4.68 mmol) was added. After addition, the resulting mixture was stirred at room temperature for 1.5 hours. Saturated ammonium chloride solution (10 mL) was added, extraction was carried out with ethyl acetate (10 mL3), and the combined organic phases were washed with saturated saline (15 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give methyl (S)-1,2,3-trimethoxy-7-(N-methylacetamido)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (111). .sup.1H NMR (DMSO-d6, 400 MHz) 7.62 (d, J=9.6 Hz, 1H), 7.11 (d, J=9.6 Hz, 1H), 6.81 (s, 1H), 6.79 (s, 1H), 4.64-4.60 (m, 1H), 3.84 (s, 3H), 3.77 (s, 6H), 3.58 (s, 3H), 3.17 (s, 3H), 2.75-2.70 (m, 1H), 2.34-2.26 (m, 2H), 2.02-1.95 (m, 4H). MS (ESI, m/z): 442.4 [M+H].sup.+.

Example 48: Synthesis of methyl (S)-7-[(N-ethylsulfonyl)amino]-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (112) and methyl (S)-7-[(N-ethylsulfonyl)amino]-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylate (113)

##STR00079##

[0200] See Step D in Example 18 for experimental operations to synthesize compounds 112 or 113 using Compound 78 (or 91) and N-ethylaminosulfonyl chloride as raw materials. Compound 112: .sup.1H NMR (DMSO-d6, 400 MHz) 7.77 (d, J=8.4 Hz, 1H), 7.64 (d, J=9.6 Hz, 1H), 7.41 (s, 1H), 7.11 (d, J=9.6 Hz, 1H), 6.80 (s, 1H), 6.77 (t, J=6.0 Hz, 1H), 3.87-3.79 (m, 10H), 3.53 (s, 3H), 2.73-2.59 (m, 3H), 2.24-2.22 (m, 1H), 2.09-1.99 (m, 1H), 1.80-1.78 (m, 1H), 0.86 (t, J=7.2 Hz, 3H). MS (ESI, m/z): 493.4 [M+H].sup.+. Compound 113: .sup.1H NMR (DMSO-d6, 400 MHz) 7.96 (d, J=8.4 Hz, 1H), 7.77 (d, J=7.2 Hz, 1H), 7.22 (d, J=12.8 Hz, 1H), 6.95 (d, J=12.8 Hz, 1H), 6.86 (s, 1H), 6.78 (t, J=6.0 Hz, 1H), 3.86-3.79 (m, 10H), 3.58 (s, 3H), 2.73-2.58 (m, 3H), 2.19-2.16 (m, 3H), 0.83 (t, J=7.2 Hz, 3H). MS (ESI, m/z): 493.4 [M+H].sup.+.

Example 49: Synthesis of methyl (S)-7-acrylamido-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (114) and methyl (S)-7-acrylamido-1,2,3-trimethoxy-10-oxo-5,6,7,10-tetrahydrobenzo[a]heptalen-9-carboxylate (115)

##STR00080##

[0201] See Step D in Example 18 for experimental operations to synthesize compounds 114 or 115 using compound 78 (or 91) and acryloyl chloride as raw materials. Compound 114: .sup.1H NMR (DMSO-d6, 400 MHz) 8.84 (d, J=7.2 Hz, 1H), 7.65 (d, J=9.6 Hz, 1H), 7.15 (d, J=9.6 Hz, 1H), 7.05 (s, 1H), 6.81 (s, 1H), 6.34-6.27 (m, 1H), 6.08-6.03 (m, 1H), 5.67-5.62 (m, 1H), 4.38-4.32 (m, 1H), 3.85 (s, 3H), 3.79 (s, 3H), 3.77 (s, 3H), 3.59 (s, 3H), 2.69-2.64 (m, 1H), 2.34-2.30 (m, 1H), 2.08-2.05 (m, 1H), 1.89-1.86 (m, 1H). MS (ESI, m/z): 440.3 [M+H].sup.+. Compound 115: .sup.1H NMR (DMSO-d6, 400 MHz) 8.82 (d, J=6.8 Hz, 1H), 7.62 (s, 1H), 7.25 (d, J=12.8 Hz, 1H), 6.96 (d, J=12.8 Hz, 1H), 6.86 (s, 1H), 6.32-6.25 (m, 1H), 6.07-6.03 (m, 1H), 5.65-5.62 (m, 1H), 4.36-4.29 (m, 1H), 3.86 (s, 3H), 3.80 (s, 3H), 3.77 (s, 3H), 3.63 (s, 3H), 2.63-2.60 (m, 1H), 2.28-2.13 (m, 3H). MS (ESI, m/z): 440.2 [M+H].sup.+.

Example 50: Synthesis of methyl (S)-7-acetamido-4-chloro-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxylate (116)

##STR00081##

[0202] The experimental operations for the synthesis of compound 116 were described in step A of Example 2, wherein the colchicine in step A of Example 2 was replaced with compound 34. .sup.1H NMR (DMSO-d6, 400 MHz) 8.65 (d, J=7.2 Hz, 1H), 7.66 (d, J=9.2 Hz, 1H), 7.13 (d, J=9.2 Hz, 1H), 7.07 (s, 1H), 4.18-4.15 (m, 1H), 3.91 (s, 3H), 3.88 (s, 3H), 3.79 (s, 3H), 3.58 (s, 3H), 3.16-3.11 (m, 1H), 2.19-2.17 (m, 1H), 1.96-1.93 (m, 1H), 1.85-1.80 (m, 4H). MS (ESI, m/z): 462.3 [M+H].sup.+.

Example 51: Synthesis of (S)N-{1,2,3-trimethoxy-9-oxo-10-(2-oxooxazolidin-3-yl]-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (118)

##STR00082##

[0203] Step A: A mixture containing colchicine (3.0 g, 7.51 mmol), ethanolamine (56.9 g, 932 mmol) and methanol (30 mL) was stirred at 30 C. for 5 hours. Water (20 mL) was added and the mixture was extracted with ethyl acetate (50 mL3). The combined organic phases were washed with saturated saline (50 mL) and dried over anhydrous sodium sulphate. The solvent was evaporated under reduced pressure and the product was purified by column chromatography (200300 mesh silica gel, eluting with ethyl acetate:methanol=9:1) to afford (S)N-{10-[(2-hydroxyethyl)amino]-1,2,3-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (117) (2.70 g). The yield was 83.9%. .sup.1H NMR (400 MHz, DMSO) 8.64 (d, J=7.6 Hz, 1H), 7.61-7.58 (m, 1H), 7.20 (d, J=11.2 Hz, 1H), 7.11 (s, 1H), 6.75-6.69 (m, 2H), 5.00-4.97 (m, 1H), 4.41-4.34 (m, 1H), 3.83 (s, 3H), 3.78 (s, 3H), 3.69-3.65 (m, 2H), 3.48 (s, 3H), 3.43-3.41 (m, 2H), 2.57-2.55 (m, 1H), 2.22-2.14 (m, 1H), 2.07-1.98 (m, 1H), 1.89-1.82 (m, 4H). MS (ESI, m/z): 429.1 [M+H].sup.+.

[0204] Step B: Compound 117 (1.0 g, 2.33 mmol) and triethylamine (708 mg, 7.0 mmol) were added to a solution of triphosgene (370 mg, 1.25 mmol) in dichloromethane (60 mL) under an ice water bath. After addition, the resulting mixture was stirred at room temperature for 30 minutes. Water (100 mL) was added and the mixture was extracted with dichloromethane (50 mL2). The combined organic phases were washed with saturated saline (30 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)N-{1,2,3-trimethoxy-9-oxo-10-(2-oxooxazolidin-3-yl]-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (118). .sup.1H NMR (400 MHz, DMSO) 8.60 (d, J=7.6 Hz, 1H), 7.69 (d, J=10.4 Hz, 1H), 7.17 (s, 1H), 7.13 (d, J=10.4 Hz, 1H), 6.80 (s, 1H), 4.47-4.43 (m, 2H), 4.36-4.29 (m, 1H), 4.10-4.04 (m, 1H), 3.94-3.90 (m, 1H), 3.85 (s, 3H), 3.80 (s, 3H), 3.57 (s, 3H), 2.67-2.61 (m, 1H), 2.31-2.22 (m, 1H), 2.08-2.00 (m, 1H), 1.86-1.80 (m, 4H). MS (ESI, m/z): 455.4 [M+H].sup.+.

Example 52: Synthesis of (S)N-{1,2,3-trimethoxy-10-(1-methyl-1H-pyrazol-4-yl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (119)

##STR00083##

[0205] To a mixture containing Compound 32 (300 mg, 0.743 mmol), 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole (232 mg, 1.11 mmol), potassium carbonate (308 mg, 2.23 mmol), water (3 mL) and dioxane (6 mL), were added 1,1-Bis(diphenylphosphino)ferrocene]dichloropalladium(II) (54 mg, 0.0743 mmol), and after addition, the resulting mixture was stirred under nitrogen at 80 C. for 4 hours. Water (20 mL) was added and the mixture was extracted with ethyl acetate (30 mL3). The combined organic phases were washed with saturated saline (20 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)N-{1,2,3-trimethoxy-10-(1-methyl-1H-pyrazol-4-yl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl}acetamide (119). .sup.1H NMR (DMSO-d6, 400 MHz) 8.60-8.57 (m, 2H), 8.11 (s, 1H), 7.92 (d, J=10.4 Hz, 1H), 7.16-7.13 (m, 2H), 6.79 (s, 1H), 4.36-4.29 (m, 1H), 3.90 (s, 3H), 3.84 (s, 3H), 3.79 (s, 3H), 3.56 (s, 3H), 2.64-2.59 (m, 1H), 2.30-2.25 (m, 1H), 2.05-2.01 (m, 1H), 1.88-1.84 (m, 4H). MS (ESI, m/z): 450.2 [M+H].sup.+.

Example 53: Synthesis of (S)-7-acetamido-1,2,3-trimethoxy-9-oxo-N-(pyridin-3-yl)-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (120)

##STR00084##

[0206] The experimental procedures for the synthesis of compound 120 using compound 46 and 3-aminopyridine as raw materials were described in step B of Example 20. .sup.1H NMR (DMSO-d6, 400 MHz) 11.36 (s, 1H), 8.84 (d, J=2.0 Hz, 1H), 8.68 (d, J=7.2 Hz, 1H), 8.33 (dd, J=1.2, 4.4 Hz, 1H), 8.19-8.16 (m, 1H), 8.08 (d, J=10.0 Hz, 1H), 7.43-7.40 (m, 1H), 7.33-7.30 (m, 2H), 6.83 (s, 1H), 4.36-4.30 (m, 1H), 3.86 (s, 3H), 3.81 (s, 3H), 3.61 (s, 3H), 2.70-2.65 (m, 1H), 2.29-2.24 (m, 1H), 2.09-2.03 (m, 1H), 1.90-1.82 (m, 4H). MS (ESI, m/z): 490.3 [M+H].sup.+.

Example 54: Synthesis of (S)-7-acetamido-2-hydroxy-1,3-dimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (121) and (S)-7-acetamido-1,3-dimethoxy-2-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (122)

##STR00085##

[0207] Step A: A mixture containing Compound 48 (200 mg, 0.469 mmol) and sulfuric acid (2 mL) was stirred at 45 C. for 7 hours. Isopropanol (5 mL) was added and the mixture was poured into ice water (10 mL). Extraction was performed with dichloromethane (20 mL3). The combined organic phases were washed with saturated sodium bicarbonate solution (20 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)-7-acetamido-2-hydroxy-1,3-dimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (121) (50.8 mg). The yield was 26.2%. .sup.1H NMR (DMSO-d6, 400 MHz) 9.29 (q, J=4.4 Hz, 1H), 8.65 (d, J=7.6 Hz, 1H), 8.21 (d, J=10.0 Hz, 1H), 7.30 (d, J=10.0 Hz, 1H), 7.23 (s, 1H), 6.70 (s, 1H), 4.35-4.28 (m, 1H), 3.82 (s, 3H), 3.50 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.59-2.55 (m, 1H), 2.20-2.19 (m, 1H), 2.02-1.99 (m, 1H), 1.84-1.79 (m, 4H). MS (ESI, m/z): 413.3 [M+H].sup.+.

[0208] Step B: A mixture containing compound 121 (100 mg, 0.242 mmol), Iodomethane-d3 (1.40 g, 9.65 mmol), potassium carbonate (101 mg, 0.731 mmol) and acetone (3 mL) was stirred at 60 C. for 1 hour. Water (10 mL) was added, and the mixture was extracted with dichloromethane (10 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)-7-acetamido-1,3-dimethoxy-2-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (122). .sup.1H NMR (DMSO-d6, 400 MHz) 9.25 (q, J=4.4 Hz, 1H), 8.63 (d, J=7.2 Hz, 1H), 8.19 (d, J=10.0 Hz, 1H), 7.29 (d, J=10.0 Hz, 1H), 7.23 (s, 1H), 6.81 (s, 1H), 4.33-4.27 (m, 1H), 3.85 (s, 3H), 3.58 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.66-2.62 (m, 1H), 2.26-2.24 (m, 1H), 2.05-2.02 (m, 1H), 1.86-1.82 (m, 4H). MS (ESI, m/z): 430.4 [M+H].sup.+.

Example 55: Synthesis of (S)-7-acetamido-2,3-dihydroxy-1-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalene-10-carboxamide (123) and (S)-7-acetamido-1-methoxy-2,3-bis(trideuteromethoxy)-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a] heptalen-10-carboxamide (124)

##STR00086##

[0209] Step A: 1 M boron tribromide dichloromethane solution (16.8 mL) was added dropwise to a solution of compound 48 (1.20 g, 2.81 mmol) in dichloromethane (138 mL) in an ice water bath. After addition, the resulting mixture was stirred at 10 C. for 4 hours. The reaction was quenched by batchwise addition of methanol (10 mL). The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to afford (S)-7-acetamido-2,3-dihydroxy-1-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptahydrotropene-10-carboxamide (123) (300 mg). The yield was 26.8%. .sup.1H NMR (DMSO-d6, 400 MHz) 9.32 (q, J=4.4 Hz, 1H), 8.62 (d, J=7.2 Hz, 1H), 8.22 (d, J=10.0 Hz, 1H), 7.30 (d, J=10.0 Hz, 1H), 7.23 (s, 1H), 6.47 (s, 1H), 4.36-4.30 (m, 1H), 3.49 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.46-2.43 (m, 1H), 2.16-2.12 (m, 1H), 2.02-1.99 (m, 1H), 1.85-1.77 (m, 4H). MS (ESI, m/z): 399.0 [M+H].sup.+.

[0210] The experimental procedures of Step B were described in Step B of Example 54 to afford (S)-7-acetamido-1-methoxy-2,3-bis(trideuteromethoxy)-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (124). .sup.1H NMR (DMSO-d6, 400 MHz) 9.25 (q, J=4.4 Hz, 1H), 8.63 (d, J=7.6 Hz, 1H), 8.19 (d, J=10.0 Hz, 1H), 7.29 (d, J=10.0 Hz, 1H), 7.23 (s, 1H), 6.80 (s, 1H), 4.33-4.27 (m, 1H), 3.58 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.66-2.62 (m, 1H), 2.26-2.24 (m, 1H), 2.05-2.02 (m, 1H), 1.87-1.82 (m, 4H). MS (ESI, m/z): 433.2 [M+H].sup.+.

Example 56: Synthesis of (S)-6-acetamido-13-methoxy-N-methyl-4-oxo-4,6,7,8-tetrahydroheptalen[1,2:4,5]benzo[1,2-d][1,3]dioxolane-3-carboxamide (125)

##STR00087##

[0211] A mixture containing compound 123 (150 mg, 0.377 mmol), chlorobromomethane (341 mg, 2.64 mmol), potassium carbonate (416 mg, 3.01 mmol) and N-methylpyrrolidone (3 mL) was stirred at 70 C. for 1 hour. After cooling to room temperature, water (5 mL) was added, and the mixture was extracted with dichloromethane (10 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)-6-acetamido-13-methoxy-N-methyl-4-oxo-4,6,7,8-tetrahydroheptalen[1,2:4,5]benzo[1,2-d][1,3]dioxolane-3-carboxamide (125) (69 mg). The yield was 44.6%. .sup.1H NMR (DMSO-d6, 400 MHz) 9.28 (q, J=4.4 Hz, 1H), 8.60 (d, J=7.6 Hz, 1H), 8.22 (d, J=10.0 Hz, 1H), 7.29 (d, J=10.0 Hz, 1H), 7.23 (s, 1H), 6.69 (s, 1H), 6.09 (dd, J=0.8, 6.8 Hz, 2H), 4.35-4.29 (m, 1H), 3.82 (s, 3H), 2.82 (d, J=4.8 Hz, 3H), 2.62-2.57 (m, 1H), 2.24-2.16 (m, 1H), 2.06-1.96 (m, 1H), 1.89-1.75 (m, 4H). MS (ESI, m/z): 411.4 [M+H].sup.+.

Example 57: Synthesis of (S)-6-acetamido-N-ethyl-13-methoxy-4-oxo-4,6,7,8-tetrahydroheptalen[1,2:4,5]benzo[1,2-d][1,3]dioxane-3-carboxamide (126) and (S)-6-acetamido-13-methoxy-4-oxo-N-phenyl-4,6,7,8-tetrahydroheptalen[1,2:4,5]benzo[1,2-d][1,3]dioxolane-3-carboxamide (127)

##STR00088##

[0212] The experimental procedures for the synthesis of compound 126 (or 127) using compound 55 (or 58) as a raw material were described in turn in step A of Example 55 and Example 56. compound 126: .sup.1H NMR (DMSO-d6, 400 MHz) 9.37 (t, J=5.6 Hz, 1H), 8.61 (d, J=7.2 Hz, 1H), 8.20 (d, J=10.0 Hz, 1H), 7.28 (d, J=10.0 Hz, 1H), 7.22 (s, 1H), 6.92 (s, 1H), 6.10 (d, J=5.6 Hz, 2H), 4.34-4.28 (m, 1H), 3.81 (s, 3H), 3.32-3.27 (m, 2H), 2.62-2.57 (m, 1H), 2.24-2.15 (m, 1H), 2.06-1.98 (m, 1H), 1.86-1.75 (m, 4H), 1.12 (t, J=7.2 Hz, 3H). MS (ESI, m/z): 425.4 [M+H].sup.+. compound 127: .sup.1H NMR (DMSO-d6, 400 MHz) 11.34 (s, 1H), 8.64 (d, J=7.6 Hz, 1H), 8.13 (d, J=10.0 Hz, 1H), 7.70 (d, J=7.6 Hz, 2H), 7.39-7.29 (m, 4H), 7.14-7.11 (m, 1H), 6.71 (s, 1H), 6.10 (d, J=5.2 Hz, 2H), 4.37-4.31 (m, 1H), 3.83 (s, 3H), 2.64-2.59 (m, 1H), 2.27-2.18 (m, 1H), 2.08-2.00 (m, 1H), 1.88-1.78 (m, 4H). MS (ESI, m/z): 473.4 [M+H].sup.+.

Example 58: Synthesis of (S)-7-acetamido-1,2-dihydroxy-3-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (128) and (S)-8-acetamido-4-methoxy-N-methyl-10-oxo-6,7,8,10-tetrahydroheptalen[1, 2:3,4]benzo[1,2-d][1,3]dioxolane-11-carboxamide (129)

##STR00089##

[0213] Step A: A mixture containing compound 48 (800 mg, 1.88 mmol) and sulfuric acid (8 mL) was stirred at 60 C. for 0.5 h, then warmed to 75 C. and continued stirring for 1.5 h. The mixture was then stirred for 1.5 h. The mixture was then heated to 75 C. and continued stirring. (S)-7-acetamido-1,2-dihydroxy-3-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (126) was obtained. After cooled to room temperature, the reaction mixture was poured batchwise into ice water (20 mL) and the pH was adjusted to 56 with 20% sodium hydroxide solution. The mixture was extracted with a solvent mixture of dichloromethane/methanol (20 mL3) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)-7-acetamido-1,2-dihydroxy-3-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (128) (370 mg). The yield was 49.4%. MS (ESI, m/z): 399.1 [M+H].sup.+.

[0214] The experimental procedures of Step B were described in Example 56 to afford (S)-8-acetamido-4-methoxy-N-methyl-10-oxo-6,7,8,10-tetrahydroheptaleno[1,2:3,4]benzo[1,2-d][1,3]dioxolane-11-carboxamide (129). .sup.1H NMR (DMSO-d6, 400 MHz) 9.16 (q, J=4.8 Hz, 1H), 8.60 (d, J=7.6 Hz, 1H), 8.17 (d, J=9.6 Hz, 1H), 7.50 (d, J=9.6 Hz, 1H), 7.22 (s, 1H), 6.67 (s, 1H), 6.14 (s, 1H), 6.08 (s, 1H), 4.40-4.34 (m, 1H), 3.88 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.70-2.63 (m, 1H), 2.31-2.26 (m, 1H), 2.11-2.08 (m, 1H), 1.92-1.85 (m, 4H). MS (ESI, m/z): 411.4 [M+H].sup.+.

Example 59: Synthesis of (S)-7-acetamido-1-hydroxy-2,3-dimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (131) and (S)-7-acetamido-2,3-dimethoxy-1-trideuteromethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (132)

##STR00090##

[0215] Step A: Tin tetrachloride (11.2 g, 42.8 mmol) was added dropwise to a solution of compound 48 (500 mg, 1.17 mmol) and acetyl chloride (3.30 g, 42.0 mmol) in dichloromethane (5 mL) in an ice water bath. After addition, the resulting mixture was stirred at 30 C. for 12 hours. Water (20 mL) was added, extraction was performed with dichloromethane (30 mL3), and the combined organic phases were washed with saturated saline (30 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure to give a crude product (900 mg) of acetic acid {(S)-7-acetamido-2,3-dimethoxy-10-methylcarbamoyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-1-yl}ester (130). This compound was used directly in the next step of the reaction without purification.

[0216] Step B: A mixture containing compound 130 crude (900 mg) and 4 M hydrochloric acid (10 mL) was stirred at 25 C. for 12 hours. Water (30 mL) was added, extraction was performed with dichloromethane (50 mL3), and the combined organic phases were washed with saturated saline (50 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to afford (S)-7-acetamido-1-hydroxy-2,3-dimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (131) (220 mg). The overall yield of the two-step reaction in steps A and B was 45.6%. MS (ESI, m/z): 413.2 [M+H].sup.+.

[0217] The experimental procedures of Step C were described in Step B of Example 54 to give (S)-7-acetamido-2,3-dimethoxy-1-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (132). .sup.1H NMR (DMSO-d6, 400 MHz) 9.25 (q, J=4.8 Hz, 1H), 8.64 (d, J=7.2 Hz, 1H), 8.19 (d, J=10.0 Hz, 1H), 7.29 (d, J=10.0 Hz, 1H), 7.22 (s, 1H), 6.80 (s, 1H), 4.31-4.28 (m, 1H), 3.84 (s, 3H), 3.79 (s, 3H), 2.80 (d, J=4.8 Hz, 3H), 2.66-2.62 (m, 1H), 2.29-2.20 (m, 1H), 2.08-2.00 (m, 1H), 1.84-1.79 (m, 4H). MS (ESI, m/z): 430.4 [M+H].sup.+.

Example 60: Synthesis of (S)-7-acetamido-2-difluoromethoxy-1,3-dimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (133)

##STR00091##

[0218] A mixture containing compound 48 (200 mg, 0.485 mmol), sodium difluorochloroacetate (74 mg, 0.485 mmol), cesium carbonate (316 mg, 0.970 mmol) and DMF (2 mL) was stirred at 70 C. for 2 hours. After cooled to room temperature, water (15 mL) was added, extraction was performed with dichloromethane (30 mL3), and the combined organic phases were washed with saturated brine (15 mL2) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was purified by preparative HPLC to give (S)-7-acetamido-2-difluoromethoxy-1,3-dimethoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (133). .sup.1H NMR (DMSO-d6, 400 MHz) 9.19 (q, J=4.4 Hz, 1H), 8.68 (d, J=7.2 Hz, 1H), 8.16 (d, J=10.0 Hz, 1H), 7.32 (d, J=10.0 Hz, 1H), 7.23 (s, 1H), 7.18 (s, 0.25H), 7.00 (d, J=2.8 Hz, 0.5H), 6.96 (s, 1H), 6.81 (s, 0.25H), 4.34-4.28 (m, 1H), 3.88 (s, 3H), 3.54 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.74-2.69 (m, 1H), 2.34-2.26 (m, 1H), 2.10-2.01 (m, 1H), 1.90-1.83 (m, 4H). MS (ESI, m/z): 463.3 [M+H].sup.+.

Example 61: Synthesis of (S)-7-acetamido-2-hydroxy-1-methoxy-3-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (134) and (S)-7-acetamido-3-hydroxy-1-methoxy-2-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (135) and (S)-7-acetamido-1,2-dimethoxy-3-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalene-10-carboxamide (136), and (S)-7-acetamido-3-difluoromethoxy-1-methoxy-2-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (137)

##STR00092##

[0219] Step A: To a solution of compound 123 (500 mg, 1.25 mmol) in DMF (5 mL) were added potassium carbonate (520 mg, 3.76 mmol) and iodomethane-d3 (200 mg, 1.38 mmol), and after addition, the resulting mixture was stirred at 50 C. for 0.5 hr. Water (20 mL) was added and extraction was performed with ethyl acetate (20 mL3). The combined organic phases were washed sequentially with water (10 mL2) and saturated saline (10 mL) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the product was separated by preparative HPLC and SFC to give (S)-7-acetamido-2-hydroxy-1-methoxy-3-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (134) and (S)-7-acetamido-3-hydroxy-1-methoxy-2-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (135). Compound 134: .sup.1H NMR (DMSO-d6, 400 MHz) 9.28 (q, J=4.4 Hz, 1H), 8.79 (s, 1H), 8.63 (d, J=7.6 Hz, 1H), 8.20 (d, J=10.0 Hz, 1H), 7.30 (d, J=10.0 Hz, 1H), 7.23 (s, 1H), 6.70 (s, 1H), 4.39-4.26 (m, 1H), 3.50 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.60-2.55 (m, 1H), 2.22-2.20 (m, 1H), 2.03-1.99 (m, 1H), 1.84-1.77 (m, 4H). MS (ESI, m/z): 416.4 [M+H].sup.+. Compound 135: .sup.1H NMR (DMSO-d6, 400 MHz) 9.77 (s, 1H), 9.28 (q, J=4.4 Hz, 1H), 8.62 (d, J=7.6 Hz, 1H), 8.20 (d, J=10.0 Hz, 1H), 7.29 (d, J=10.0 Hz, 1H), 7.22 (s, 1H), 6.58 (s, 1H), 4.34-4.28 (m, 1H), 3.57 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.60-2.55 (m, 1H), 2.19-2.17 (m, 1H), 2.02-1.99 (m, 1H), 1.84-1.75 (m, 4H). MS (ESI, m/z): 416.4 [M+H].sup.+.

[0220] The experimental procedures of step B were described in Example 54, wherein the Iodomethane-d3 in step B of Example 54 was replaced with iodomethane to give (S)-7-acetamido-1,2-dimethoxy-3-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (136). .sup.1H NMR (DMSO-d6, 400 MHz) 9.25 (q, J=4.4 Hz, 1H), 8.64 (d, J=7.6 Hz, 1H), 8.20 (d, J=9.6 Hz, 1H), 7.29 (d, J=9.6 Hz, 1H), 7.23 (s, 1H), 6.80 (s, 1H), 4.33-4.27 (m, 1H), 3.79 (s, 3H), 3.58 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.66-2.62 (m, 1H), 2.26-2.24 (m, 1H), 2.05-2.02 (m, 1H), 1.87-1.82 (m, 4H). MS (ESI, m/z): 430.4 [M+H].sup.+.

[0221] The experimental procedures of Step C were described in Example 60 to afford (S)-7-acetamido-3-difluoromethoxy-1-methoxy-2-trideutero-methoxy-N-methyl-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-10-carboxamide (137). .sup.1H NMR (DMSO-d6, 400 MHz) 9.17 (q, J=4.4 Hz, 1H), 8.66 (d, J=7.2 Hz, 1H), 8.16 (d, J=10.0 Hz, 1H), 7.41 (s, 0.25H), 7.31 (d, J=10.0 Hz, 1H), 7.22-7.21 (m, 1.5H), 7.04 (s, 0.25H), 6.94 (s, 1H), 4.30-4.24 (m, 1H), 3.59 (s, 3H), 2.81 (d, J=4.8 Hz, 3H), 2.70-2.65 (m, 1H), 2.27-2.23 (m, 1H), 2.03-2.00 (m, 1H), 1.87-1.82 (m, 4H). MS (ESI, m/z): 466.0 [M+H].sup.+.

Example 62: Pharmacodynamics Experiment a of Compounds on Gouty Arthritis in Rats

1. Experimental Materials

(1) Test Drug

[0222] Compounds 2, 17, 26 and 27 were prepared by grinding with 0.5% CMC-Na into a suspension of the corresponding concentration (0.06 mg/mL) for gavage before administration, and the volume of administration was 10 mL/kg. Colchicine was purchased from Sam Chemical Technology (Shanghai) Co. with a batch number 49ETRRAS. Before use, 0.5% CMC-Na was used for grinding and prepared into a suspension of the corresponding concentration (0.06 mg/mL) for gavage, and the volume of drug administered was 10 mL/kg.

(2) Experimental Animals

[0223] Sprague Dawley (SD) rats, SPF grade, male, weighing 180-240 g, were purchased from Zhejiang Vital River Laboratory Animal Technology Co., Ltd., under License No.: SCXK (Zhejiang).sub.2019-0001; Animal Qualification Certificate No.: 20210119Aazz0619000545 (Zhejiang).

2 Experimental Methods

(1) Grouping Method

[0224] 70 SD rats, males, weighing 180-240 g, were randomly divided into 7 groups of 10 animals each: blank control group (0.5% CMC-Na), model group (0.5% CMC-Na), positive control colchicine group, compound 2 group, compound 17 group, compound 26 group, and compound 27 group, and the administered dose was 0.6 mg/kg. Each group of subjects was prepared into a suspension of the corresponding concentration, and the administration volume was 10 mL/kg.

(2) Modeling and Drug Administration

[0225] The crystalline arthritis model was prepared by intra-articular injection of urate crystals (40 mg/mL, in the left ankle joint of each rat). Different doses of the test drug were given separately by gavage administration. The day of administration was defined as day 1 of the experiment, and the drug was administered once a day for 10 consecutive days (pre-administration of the drug for 7 days prior to modeling, modeling on day 8, and continuation of the drug for 3 days). The experiment ended on day 10.

(3) Test Indicators

a. Paw Volume

[0226] Paw volume was measured before administration, before urate injection, and 2, 4, 8, 10, 24, and 48 h after injection.

b. Gait Score

[0227] The degree of lameness of the modeled joints was observed and scored at the same time point.

c. Pain Score

[0228] The pain level was scored using the flexion and extension joint pain test scoring method at the same time point.

(4) Data Processing and Statistical Methods

[0229] The data obtained from the experiment were statistically analyzed using IBM SPSS Statistics 22.0. All measurements were expressed as MeanS.E.M s. The data were analyzed using SPSS 22.0 statistical software and variance chi-square test was performed on the parameters.

3. Experimental Results

(1) Paw Volume

[0230] The paw volume data of rats in each group are shown in Table 1. At each time point after modeling, the paw volume of rats in the model group was significantly larger than that of the blank control group (P<0.001). The paw volumes of rats in the colchicine group were significantly smaller than those in the model group at 2 h, 4 h, 8 h, 10 h, and 48 h after modeling (P<0.01). Each test compound group could reduce the paw volume of rats at different time points after modeling, among which, compounds 2 and 26 could significantly reduce the paw volume at 2 h, 4 h, and 48 h, compound 17 could significantly reduce the paw volume at 2 h, 8 h, 24 h, and 48 h, and compound 27 could significantly reduce the paw volume at 2 h, 4 h, 8 h, 10 h, and 48 h.

TABLE-US-00001 TABLE 1 Paw volume (cm.sup.3) for each group (Mean SEM) Compound Dose name (mg/ Before or No. kg) modeling 2 h 4 h 8 h 10 h 24 h 48 h blank 2.18 0.04 2.15 0.02 2.15 0.01 2.14 0.02 2.13 0.02 2.15 0.02 2.14 0.03 control group model group 2.22 0.03 .sup.2.71 0.05.sup.### .sup.2.67 0.04.sup.### .sup.2.66 0.04.sup.### .sup.2.64 0.03.sup.### .sup.2.60 0.03.sup.### 2.52 0.04.sup.### colchicine 0.6 2.18 0.03 2.44 0.02*** 2.45 0.03** 2.44 0.04*** 2.46 0.04** 2.46 0.05 2.24 0.03*** 2 0.6 2.18 0.02 2.51 0.06* 2.54 0.04* 2.63 0.06 2.59 0.07 2.52 0.08 2.35 0.06* 17 0.6 2.19 0.03 2.54 0.02** 2.58 0.02 2.54 0.04* 2.55 0.03 2.41 0.05** 2.23 0.03*** 26 0.6 2.19 0.04 2.53 0.04* 2.54 0.03* 2.55 0.04 2.57 0.05 2.47 0.06 2.34 0.07* 27 0.6 2.16 0.04 2.51 0.04** 2.54 0.03* 2.57 0.04* 2.55 0.04* 2.50 0.07 2.29 0.04** Note: ###P < 0.001, compared with the blank control group at the same time point; *P < 0.05, **P < 0.01, ***P < 0.001, compared with the model group at the same time point.

(2) Gait Score

[0231] The gait scores of rats in each group were shown in Table 2. At each time point after modeling, the gait scores of rats in the model group were significantly higher than those in the blank control group (P<0.001). The colchicine group improved the gait of rats at 2 h, 24 h, and 48 h after modeling (P<0.05 or P<0.01). Each tested compound group could improve the gait of rats at different time points after modeling, among which, compound 2 significantly improved the gait at 2 h, 8 h, 24 h, and 48 h, compounds 17 and 26 significantly improved the gait at 2 h, 8 h, and 24 h, and compound 27 significantly improved the gait at 2 h, 24 h, and 48 h (P<0.05 or P<0.01).

TABLE-US-00002 TABLE 2 Effect of compounds on gait scores in rats with gouty arthritis (Mean SEM) Compound Dose Before name or No. (mg/kg) modeling 2 h 4 h 8 h 10 h 24 h 48 h blank 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 control group model 0.00 0.00 .sup.1.60 0.16.sup.### .sup.1.30 0.1.sup.### .sup.1.70 0.1.sup.### .sup.1.40 0.1.sup.### 1.60 0.1.sup.### .sup.1.50 0.17.sup.### group colchicine 0.6 0.00 0.00 1.10 0.10* 1.10 0.10 1.30 0.15 1.30 0.21 1.00 0.00** 1.00 0.00* 2 0.6 0.00 0.00 1.10 0.10* 1.10 0.10 1.10 0.10** 1.20 0.13 1.00 0.00** 1.00 0.00* 17 0.6 0.00 0.00 1.00 0.00** 1.20 0.13 1.00 0.00** 1.10 0.10 1.10 0.10* 1.10 0.10 26 0.6 0.00 0.00 1.00 0.00** 1.10 0.10 1.20 0.13* 1.30 0.15 1.10 0.10** 1.10 0.10 27 0.6 0.00 0.00 1.00 0.00** 1.10 0.10 1.40 0.22 1.20 0.20 1.00 0.00** 1.00 0.00* Note: .sup.####P < 0.001, compared with the normal control group at the same time point; *P < 0.05, **P < 0.01, compared with the model group at the same time point.

(3) Pain Scores

[0232] The pain scores of rats in each group were shown in Table 3. At each time point after modeling, the pain scores of rats in the model group were significantly higher than those in the blank control group (P<0.001). The colchicine group improved the pain at 2 h, 8 h, 24 h, and 48 h (P<0.05 or P<0.01). Each test compound group could improve rat pain at different time points after modeling, among which, compound 2 significantly improved pain at 2 h, 4 h, 8 h, 10 h, 24 h, and 48 h, compound 17 significantly improved gait at 2 h, 8 h, and 24 h, compound 26 significantly improved gait at 2 h, and compound 27 significantly improved gait at 2 h, 8 h, 10 h, 24 h, and 48 h (P<0.05 or P<0.01).

TABLE-US-00003 TABLE 3 Pain scores of rats in each group (Mean SEM) Com- pound Dose name (mg/ Before or No. kg) modeling 2 h 4 h 8 h 10 h 24 h 48 h blank 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 control group model 0.00 0.00 .sup.4.80 0.13.sup.### .sup.4.20 0.33.sup.### .sup.4.80 0.13.sup.### .sup.4.20 0.36.sup.### .sup.3.60 0.56.sup.### .sup.3.50 0.62.sup.### group colchicine 0.6 0.00 0.00 3.30 0.60* 2.90 0.71 3.20 0.61* 2.60 0.70 1.40 0.58* 0.80 0.44** 2 0.6 0.00 0.00 2.70 0.68* 1.30 0.52*** 3.30 0.54* 2.20 0.68* 1.30 0.62* 1.00 0.67* 17 0.6 0.00 0.00 3.10 0.64* 3.20 0.66 3.00 0.68* 3.30 0.54 1.30 0.65* 1.60 0.70 26 0.6 0.00 0.00 3.00 0.56* 2.90 0.67 4.30 0.40 3.80 0.59 3.20 0.51 2.20 0.73 27 0.6 0.00 0.00 2.00 0.67** 2.80 0.68 3.30 0.47* 2.50 0.65* 1.50 0.60* 1.00 0.60** Note: .sup.####P < 0.001, compared with blank control group at the same time point; *P < 0.05, **P < 0.01, compared with model group at the same time point.

Example 63: Pharmacodynamics Experiment B of Compounds on Gouty Arthritis Rats

1. Experimental Materials

(1) Test Drug

[0233] Compounds 12, 16, 19, 30, 34, 36, 38, 40, 44, 48, 49, 52, 58, 69, 83, 98, 102, 119, 121, and 122 were prepared by grinding with 0.5% CMC-Na prior to administration and formulated into suspensions of appropriate concentration (0.12 mg/mL) for gavage, with an administered dose of 0.5 mL/100 g. Colchicine, purchased from MCE Company, with Lot No. 41632, before use, was prepared by grinding with 0.5% CMC-Na and prepared into a suspension of corresponding concentration (0.12 and 0.24 mg/mL) for gavage, and the volume of administration was 0.5 mL/100 g.

(2) Experimental Animals

[0234] Sprague Dawley (SD) rats, SPF grade, male, weighing 180-220 g, were purchased from slac laboratory animal Co. Ltd, Production License No.: SCXK (Shanghai) 2017-0005, Quality Certificate No.: 20170005054931.

(3) Experimental Reagents

[0235] Sodium urate, purchased from Sigma, saline containing 10% Tween-80 was used to formulate a suspension with a concentration of 25 mg/mL.

2. Experimental Methods

(1) Grouping Method

[0236] 240 SD rats, males, were acclimatized for one week and weighed 180-220 g. They were randomly divided into 24 groups of 10 rats each: blank control group (0.5% CMC-Na), model group (0.5% CMC-Na), positive control colchicine low-dose group (0.6 mg/kg), positive control colchicine high-dose group (1.2 mg/kg), and each compound groups. The dose of each compound group was 0.6 mg/kg. The compounds in each group were prepared into the corresponding concentration suspension, and the volume of administration was 10 mL/kg.

(2) Modeling and Dosing Regimen

[0237] 250 mg of sodium urate were added to 9 mL of saline, and 1 mL of Tween-80 solution was added, the resulting mixture was heated and stirred to formulate 10 mL of sodium urate solution. The rats were grasped and fixed, and 0.2 mL of sodium urate solution was injected into the right ankle and knee joint cavities respectively to establish gouty arthritis rat model.

[0238] Before modeling, positive drug and tested compounds were pre-administered for 7 days, once a day, and modeling was performed on day 8, and drug administration by gavage continued for 3 days after modeling.

3. Test Indicators

(1) Gait Score

[0239] Gait was scored according to the modified gait grading criteria introduced by Coderre et al. The gait of each rat was observed before and 2 h, 4 h, 8 h, 10 h, 24 h and 48 h after modeling and graded scoring was performed. The gait grading criteria were as follows: 0 point, normal walking; 1 point, slight lameness, the lower limbs were slightly bent; 2 points: moderate lameness, the lower limbs just touched the ground; 3 points, severe lameness, the lower limbs left the ground, and walked on three feet. The mean score of each group was calculated and compared with that of the model group.

(2) Ankle Swelling Rate

[0240] Before modeling and 2 h, 4 h, 8 h, 10 h, 24 h and 48 h after modeling, the circumference of the right ankle joint was measured using a soft ruler or the volume change at the ankle was measured (volumetric method), and the swelling degree and swelling rate of ankle joint were calculated:

Swelling degree (mm)=measured circumference (mm)baseline circumference (mm)

Swelling rate (%)=swelling degree (mm)/baseline circumference (mm)100%

Data Processing and Statistical Methods

[0241] Each experimental data was expressed as the mean, and intergroup comparisons were examined for significance using the ANOVA-Dunnett T-test, with P<0.05 as the significance indicator.

4. Experimental Results

(1) Gait Score

[0242] The gait scores of rats in each group were shown in Table 4. Compared with the control group, the gait scores of the model group were significantly higher at 8 h and 10 h after modeling (P<0.05), but the gait began to recover at 24 h after modeling. At 4 h, 8 h, 10 h and 24 h after modeling, compared with the model group, colchicine low-dose and high-dose groups significantly improved the gait of rats (P<0.05), and significant improvement in gait occurred at different time points in compound 12, 16, 19, 30, 34, 36, 38, 40, 44, 48, 49, 52, 58, 69, 83 and 98 groups (P<0.05 or P<0.01).

TABLE-US-00004 TABLE 4 Effect of compounds on gait scores in gouty arthritis rats Compound Dose Gait Score name or No. (mg/kg) 0 h 2 h 4 h 8 h 10 h 24 h 48 h Blank / 0 0 0 0 0 0 0 control group model / 0 0 0.17 0.45.sup.# 0.53.sup.# 0.20 0.08 group colchicine 0.6 0 0 0.02* 0.24* 0.16** 0.02* 0.02 low dose group colchicine 1.2 0 0 0** 0.1** 0.18** 0* 0 high dose group 12 0.6 0 0 0 0* 0* 0 0 16 0.6 0 0 0.1 0* 0* 0 0 19 0.6 0 0.0 0 0** 0** 0.2 0.1 30 0.6 0 0 0 0* 0* 0.3 0 34 0.6 0 0 0 0** 0.1** 0 0 36 0.6 0 0 0 0* 0* 0.2 0.1 38 0.6 0 0 0 0* 0* 0 0 40 0.6 0 0 0 0* 0* 0.4 0 44 0.6 0 0 0 0* 0* 0 0 48 0.6 0 0 0.1 0.1** 0** 0* 0 49 0.6 0 0 0 0* 0* 0.2 0 52 0.6 0 0 0 0* 0* 0 0 58 0.6 0 0 0* 0.3 0.2** 0 0 69 0.6 0 0 0 0* 0* 0 0 83 0.6 0 0 0 0* 0* 0 0 98 0.6 0 0 0 0* 0* 0.1 0 102 0.6 0 0 0 0.27 0.09* 0 0 119 0.6 0 0 0 0.1* 0.4 0. 0 121 0.6 0 0 0 0.2 0.2 0 0 122 0.6 0 0 0.2 0.1 0.2 0.1 0 Note: .sup.#P < 0.05, compared with the blank control group at the same time point; *P < 0.05, **P < 0.01, compared with the model group at the same time point.

(2) Ankle Joint Swelling Rate

[0243] The ankle joint swelling rate of rats in each group were shown in Table 5. Compared with the blank control group, the ankle joint swelling rate was significantly higher in the model group at each time point after modeling (P<0.01). The ankle swelling rate of rats in colchicine low and high dose groups were significantly (P<0.01) reduced after modeling compared with the model group at the same time point. The ankle swelling rate of rats in all the tested compounds groups at different time points were significantly reduced after modeling (P<0.05 or P<0.01).

TABLE-US-00005 TABLE 5 Effect of compounds on ankle swelling rate in gouty arthritis rats (x) Compound Dose Swelling rate name or No. (mg/kg) 2 h 4 h 8 h 10 h 24 h 48 h blank / 1.66% 1.28% 2.24% 2.39% 2.05% 1.29% control group model / 15.72%.sup.## 19.75%.sup.## 27.05%.sup.## 32.66%.sup.## 31.26%.sup.## 19.07%.sup.## group colchicine 0.6 10.49%** 15.73%* 20.61%** 23.39%** 24.38%** 12.11%** low dose group colchicine 1.2 8.97%** 14.92%** 17.18%** 19.37%** 19.82%** 9.29%** high dose group 12 0.6 10.77% 16.53% 18.70%* 31.35% 22.77%* 15.01% 16 0.6 9.71% 12.97%* 17.78%** 17.38%** 18.59%** 6.17%** 19 0.6 8.77%* 10.88%** 17.09%** 14.05%** 19.45%** 7.77%** 30 0.6 4.04%** 1.20%** 0.57%** 6.69%** 9.77%** 1.34%** 34 0.6 3.98%** 8.32%** 16.22%** 17.30%** 21.05%** 9.96%** 36 0.6 3.59%** 2.37%** 2.62%** 10.45%** 10.03%** 2.54%** 38 0.6 6.34%** 12.63%* 10.41%** 17.87%** 16.90%** 6.71%** 40 0.6 5.81%** 7.96%** 11.21%** 9.90%** 21.73%* 9.33%* 44 0.6 9.72%** 8.92%** 6.13%** 1.47%** 3.17%** 6.20%** 48 0.6 9.42%** 15.52%* 21.03%* 21.47%** 22.39%** 13.60%* 49 0.6 1.02%** 7.38%** 8.12%** 16.54%** 12.89%** 1.43%** 52 0.6 10.98% 9.83%* 11.79%** 30.20% 21.98%* 7.88%* 58 0.6 6.48%** 13.85%** 17.19%** 20.43%** 18.13%** 8.42%** 69 0.6 5.65%** 7.01%** 14.66%** 26.82%* 19.00%* 5.21%** 83 0.6 8.31%** 10.38%** 16.57%** 21.13%* 26.26% 15.28% 98 0.6 7.9%** 13.4%* 16.4%** 17.7%** 19.7%* 8.9%* 102 0.6 14.1% 13.6%** 14.4%** 19.3%** 16.7%** 9.4%* 119 0.6 10.4% 14.1%** 19.4%* 22.2%* 15.8%** 5.3%** 121 0.6 11.8% 15.6%* 20.9% 22.5%* 16.3%** 9.4%* 122 0.6 7.9%** 12.1%** 15.8%** 15.3%** 12.5%** 4.7%** Note: .sup.##P < 0.01, compared with blank control group at same time point; *P < 0.05, **P < 0.01, compared with model group at same time point.

Example 64: Pharmacodynamics Experiment a of Compounds on Inflammatory Factor Levels

1. Experimental Materials

(1) Test Drugs

[0244] Compounds 14, 17 and 26 were prepared by grinding with 0.5% CMC-Na before administration and into a suspension for gavage at the corresponding concentration (0.06 mg/mL), with a dosage volume of 10 mL/kg. Colchicine, purchased from Sun Chemical Technology (Shanghai) Co., Ltd. with batch no. 49ETRRAS, and was prepared by grinding with 0.5% CMC-Na before administration and into a suspension for gavage at the corresponding concentration (0.06 mg/mL), with a dosage volume of 10 mL/kg.

(2) Experimental Animals

[0245] Sprague Dawley (SD) rats, SPF grade, male, weighing 180-240 g, were purchased from Zhejiang Vital River Laboratory Animal Technology Co., Ltd., with License No.: SCXK (Zhe) 2019-0001; Animal Qualification Certificate No.: 20210119Aazz0619000545 (Zhejiang).

2. Experimental Methods

(1) Grouping Method

[0246] Sixty SD rats, males, weighing 180-240 g, were randomly divided into six groups of 10 animals each: blank control group (0.5% CMC-Na), model group (0.5% CMC-Na), positive control colchicine group, compound 14 group, compound 17 group, and compound 26 group, and the administered dose was all 0.6 mg/kg. The compounds in each group were prepared into a suspension of the corresponding concentration, and the dosage volume was 10 mL/kg.

(2) Modeling and Dosing

[0247] The crystalline arthritis model was prepared by intra-articular injection of urate crystals (40 mg/mL in the left ankle joint of each rat). Different doses of the test compounds were given separately by gavage administration. The day of administration was defined as day 1 of the experiment, and the compounds were administered once a day for 10 consecutive days (pre-administration of the drug for 7 days prior to modeling, modeling on day 8, and continuation of the drug for 3 days). The experiment ended on day 10.

(3) Test Indicators

a. Inflammatory Factor

[0248] At the end of the experiment (day 10), animals were dissected, and the synovial leachate from the ankle joint cavity at the modeling site was taken to test the levels of IL-6 and TNF-.

(4) Data Processing and Statistical Methods

[0249] The data obtained from the experiment were statistically analyzed using IBM SPSS Statistics 22.0. The data were analyzed using SPSS 22.0 statistical software, and the parameters were subjected to variance chi-square test.

3. Experimental Results

(1) TNF-

[0250] The results of the decreased percentage of TNF- in the synovial tissue of the knee joint of rats in each group are shown in Table 6. Colchicine can reduce the TNF- level in the synovial tissue of rat knee joints after modeling. All compound could reduce the TNF- level in the synovial membrane of rat knee joints after modeling.

TABLE-US-00006 TABLE 6 Effect of compounds on TNF- in synovial tissue of knee joints of gouty arthritis rats Compound name Dose Decreased TNF- or No. (mg/kg) percentage % Colchicine 0.6 93.73 14 0.6 125.78 17 0.6 107.16 26 0.6 160.36

(2) IL-6

[0251] The results of the decreased percentage of IL-6 in the synovial tissue of the knee joint of rats in each group were shown in Table 7. Colchicine reduced the IL-1 level in the synovial tissue of the knee joint of rats after modeling. All compounds decreased the IL-6 level in the synovial tissue of the knee joint of rats after modeling.

TABLE-US-00007 TABLE 7 Effect of compounds on IL-6 in synovial tissue of knee joints of gouty arthritis rats Compound name Dose Decreased IL-6 or No. (mg/kg) percentage % colchicine 0.6 107.14 14 0.6 119.58 17 0.6 132.54 26 0.6 113.37

Example 65: Pharmacodynamics Experiment B of Compounds on Levels of Inflammatory Factors

1. Experimental Materials

(1) Test Drugs

[0252] Compounds 19, 34, 38, 44, 48, 49, 55, 58, 60, 61, 69, 81, 95, 97, 98, 99, 100, 108, 112, 119, 121, and 122 were prepared by grinding with 0.5% CMC-Na prior to administration and formulated into suspensions of appropriate concentration (0.12 mg/mL) for gavage, with the administration volume of 0.5 mL/100 g. Colchicine, purchased from MCE Company with Lot No. 41632, was prepared by grinding with 0.5% CMC-Na and prepared into a suspension of the corresponding concentration (0.12 and 0.24 mg/mL) for gavage before use, and the volume of administration was 0.5 mL/100 g.

(2) Experimental Animals

[0253] Sprague Dawley (SD) rats, SPF grade, male, weighing 180-220 g, were purchased from slac laboratory animal co. ltd with Production License No.: SCXK (Shanghai) 2017-0005, Quality Certificate No.: 20170005054931.

(3) Experimental Reagents

[0254] Sodium urate, purchased from Sigma, was used to prepare a suspension with a concentration of 25 mg/mL using saline containing 10% Tween-80. IL-1 and TNF- detection kits (ELISA method) were purchased from Wuhan Elabscience Biotechnology Co., Ltd. IL-6 detection kits (ELISA method) were purchased from Hangzhou MultiSciences Biotech Co., Ltd.

2. Experimental Methods

(1) Grouping Method

[0255] 260 SD rats, male, one week after acclimatization, weighing 180-220 g, were randomly divided into 26 groups of 10 rats each: blank control group (0.5% CMC-Na), model group (0.5% CMC-Na), positive control colchicine low-dose group (0.6 mg/kg), positive control colchicine high-dose group (1.2 mg/kg), and the test Compound group. The dose of compound group was 0.6 mg/kg. The compound in each group were prepared into the corresponding concentration suspension, and the dosage volume was 10 mL/kg.

(2) Modeling and Dosing Regimen

[0256] 250 mg of sodium urate were added to 9 mL of saline, and 1 mL of Tween-80 solution was added, and the resulting mixture was heated and stirred to formulate 10 mL of sodium urate solution. The rats were grasped and fixed, and 0.2 mL of sodium urate solution was injected into the right ankle and knee joint cavities respectively to establish gouty arthritis rat model.

[0257] Positive drug and test compounds were pre-administered for 7 days before modeling, once a day, modeling was performed on day 8, and administration by gavage continued for 3 days after modeling.

(3) Test Indicators

(a) Inflammatory Factors

[0258] Measurement of IL-1, IL-6 and TNF- in synovial tissue: 48 h after modeling, rats were killed by decapitation, synovial tissues of the knee joints were dissected to prepare tissue homogenates, and ELISA kit was used to measure the level of IL-1, IL-6 and TNF- in synovial tissues, and the percentage decrease of each inflammatory factor was calculated.

Data Processing and Statistical Methods

[0259] The data of each experimental measure were expressed as percentage decrease.

3. Experimental Results

(1) TNF-

[0260] The results of the decreased percentage of TNF- in the synovial tissue of the knee joint of rats in each group were shown in Table 8. Both low-dose and high-dose groups of colchicine could reduce the TNF-level of synovial tissue of rat knee joints after modeling. All compound groups could reduce the TNF- level in the synovial tissue of the knee joint of rats after modeling, and the inhibition of the TNF- level in the synovial tissue of the knee joint of rats after modeling was significantly better than that of colchicine.

TABLE-US-00008 TABLE 8 Effect of compounds on TNF- in synovial tissue of knee joints of gouty arthritis rats TNF- Decreased Compound Dose percentage Compound Dose TNF- name or No. (mg/kg) decrease % name or No. (mg/kg) percentage % colchicine 0.6 57.97 69 0.6 76.08 low dose group colchicine 1.2 52.46 81 0.6 77.98 high dose group 19 0.6 84.70 95 0.6 70.03 34 0.6 82.86 97 0.6 92.04 38 0.6 86.57 98 0.6 98.89 44 0.6 72.57 99 0.6 91.73 48 0.6 81.58 100 0.6 119.69 49 0.6 79.18 108 0.6 103.68 55 0.6 86.42 112 0.6 97.82 58 0.6 80.45 119 0.6 104.77 60 0.6 85.83 121 0.6 119.21 61 0.6 71.23 122 0.6 98.89

(2) IL-1

[0261] The results of the decreased percentage of IL-1 in the synovial tissue of the knee joint of rats in each group were shown in Table 9. Colchicine low-dose and high-dose groups reduced IL-1 in synovial tissue of rat knee joints after modeling. All compound groups could reduce the mL-1 level in the synovial tissue of the knee joint of rats after modeling, and the inhibition of IL-1 level in the synovial tissue of the knee joint of rats by most compounds was significantly better than that of colchicine.

TABLE-US-00009 TABLE 9 Effect of compounds on IL-1 in synovial tissue of knee joints of gouty arthritis rats IL-1 Decreased Compound Dose percentage Compound Dose IL-1 name or No. (mg/kg) decrease % name or No. (mg/kg) percentage % Colchicine 0.6 74.20 69 0.6 109.93 low dose group colchicine 1.2 63.07 81 0.6 86.60 high dose group 19 0.6 81.53 95 0.6 40.40 34 0.6 93.07 97 0.6 20.24 38 0.6 102.09 98 0.6 64.98 44 0.6 104.38 99 0.6 66.42 48 0.6 82.15 100 0.6 65.22 49 0.6 69.07 108 0.6 92.74 55 0.6 132.97 112 0.6 91.96 58 0.6 87.55 119 0.6 85.49 60 0.6 76.08 121 0.6 67.70 61 0.6 97.21 122 0.6 86.87

(3) IL-6

[0262] The results of the decreased percentage of IL-6 in the synovial tissue of the knee joint of rats in each group were shown in Table 10. Colchicine low-dose and high-dose groups could reduce the IL-6 level of knee synovial tissue after modeling. All compound groups could reduce the IL-6 level in the synovial tissue of the knee joint of rats after modeling, and the inhibition of IL-6 level in the synovial tissue of the knee joint of rats after modeling by most of the compounds was significantly better than that of colchicine.

TABLE-US-00010 TABLE 10 Effect of compounds on IL-6 in synovial tissue of knee joints of gouty arthritis rats IL-6 Decreased Compound Dose percentage Compound Dose IL-6 name or No. (mg/kg) decrease % name or No. (mg/kg) percentage % Colchicine 0.6 83.54 69 0.6 83.05 low dose group Colchicine 1.2 96.32 81 0.6 70.64 high dose group 19 0.6 98.08 95 0.6 109.36 34 0.6 115.12 97 0.6 97.81 38 0.6 68.98 98 0.6 167.41 44 0.6 19.72 99 0.6 79.57 48 0.6 109.78 100 0.6 168.80 49 0.6 78.58 108 0.6 207.01 55 0.6 128.11 112 0.6 165.11 58 0.6 86.72 119 0.6 169.36 60 0.6 59.51 121 0.6 231.24 61 0.6 52.45 122 0.6 220.07

Example 66: Acute Toxicity Test of Compounds 34, 48, 55 and 58 in Mice by Single Administration

1. Experimental Materials

[0263] Compounds 34, 48, 55 and 58 were ground with 0.5% CMC-Na before administration, and prepared into a suspension of the corresponding concentration for gavage, with an administration volume of 0.1 mL/10 g. Colchicine, purchased from Sun Chemical Technology (Shanghai) Co., Ltd. with batch number 49ETRRAS, was ground using 0.5% CMC-Na before use, and formulated into appropriate concentration of suspension for gavage with an administration volume of 0.1 mL/10 g.

2. Test Animals and Feeding Conditions

[0264] ICR mice, SPF grade, weighing 18-20 g, 6-8 weeks old, were provided by Nantong University. The Laboratory Animal Production License was SCXK (Su) 2016-0010 and Laboratory Animal Use License was SYXK (Su) 2017-0035.

3. Experimental Methods and Results

[0265] ICR mice were randomly divided into 18 groups, i.e., normal group, colchicine group (50 mg/kg), 50 mg/kg dose group, 100 mg/kg dose group, 400 mg/kg dose group and 600 mg/kg dose group of compound 34, 50 mg/kg dose group, 100 mg/kg dose group, 400 mg/kg dose group and 600 mg/kg dose group of compound 48, 50 mg/kg dose group, 100 mg/kg dose group, 400 mg/kg dose group and 600 mg/kg dose group of Compound 55, 50 mg/kg dose group, 100 mg/kg dose group, 400 mg/kg dose group and 600 mg/kg dose group of Compound 58, with three females and three males in each group. After overnight fasting, the drug was administered once by gavage in a volume of 0.1 mL/10 g. The normal group was given the corresponding volume of 0.5% CMC-Na solution according to body weight. The state of the animal was observed after administration, the animals were weighed daily and the number of deaths was recorded for 14 consecutive days.

[0266] The dose and mortality rate of mice in each group were shown in Table 11. No immediate toxic reaction was observed in each compound group, no delayed toxic reaction was observed from 24 hours to 14 days, the animals were in good condition with weight gain and all the mice survived. The maximum tolerated dose (MTD) for acute toxicity testing in mice was greater than 600 mg/kg for compounds 34, 48, 55 and 58, whereas the MTD for colchicine was less than 50 mg/kg.

TABLE-US-00011 TABLE 11 Doses administered to ICR mice and mortality rate Dose Animal Mortality Group (mg/kg) No. rate normal / 6 0/6 group colchicine 50 6 3/6 group compound 50 6 0/6 34 100 6 0/6 400 6 0/6 600 6 0/6 compound 50 6 0/6 48 100 6 0/6 400 6 0/6 600 6 0/6 compound 50 6 0/6 55 100 6 0/6 400 6 0/6 600 6 0/6 compound 50 6 0/6 58 100 6 0/6 400 6 0/6 600 6 0/6

Example 67: In Vivo Pharmacokinetics of Compounds 48, 55, 56, 58, 69, 124, 125 and 132 in SD Rats

1. Experimental Materials

(1) Test Drugs

[0267] Compound stock solution preparation: an appropriate amount of solid powder of the compounds were weighed separately, a certain amount of DMSO was added and 20 mg/mL of stock solution was obtained under vortex ultrasound.

[0268] Preparation of test compound for gavage: an appropriate amount of compound stock solution was taken by pipette, a certain amount of Solutol HS15 solution was added, the mixture was vortexed for 1 minute, then a certain amount of saline was added, and 1 mg/mL of solution was obtained after mixing well.

[0269] Preparation of test compounds for intravenous injection: an appropriate amount of compound stock solution was taken by pipette separately, a certain amount of Solutol HS15 solution was added, the mixture was vortexed for 1 minute, then a certain amount of physiological saline was added and a solution of 0.5 mg/mL was obtained after mixing well.

(2) Experimental Animals

[0270] SD rats, male, SPF grade, 6-8 weeks old, were purchased from JH Laboratory Animal Co. LTD. The license No. was SCXK (SH) 2017-0012 and the Certificate of Conformity No. was 20170012022077.

2. Experimental Methods

(1) Dose and Mode of Administration

[0271] The animals were fasted overnight before administration by gavage, and food was given 4 hours after administration, during which they were free to drink. Two groups were set for each test compound, intravenous injection group and oral administration group, and the dose and mode of administration were shown in Table 12 below.

TABLE-US-00012 TABLE 12 Dose and mode of administration of compounds to SD rats Dose Volume Concentration Mode of Group (mg/kg) (mL/kg) (mg/mL) administration intravenous 1 2 0.5 intravenous injection group oral 10 10 1 oral administration group

(2) Experimental Operations

[0272] Blood samples (150 L/sample) were collected from the jugular vein of SD rats before and 5 min (intravenous injection group only), 15 min, 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, and 24 h after administration, respectively, and were placed in centrifuge tubes containing the anticoagulant sodium heparin. The blood samples were centrifuged at 4 C. for 5 min at 2,000 g to separate the plasma. The plasma samples were analyzed by LC/MS/MS to detect the concentration of each test compound in the plasma samples.

Pharmacokinetic Analysis

[0273] The parameters related to the non-atrial model were obtained by calculations with WinNonlin Professional software.

[00001]$\mathrm{Bioavailability}F\%=\left({\mathrm{Dose}}_{\left(\mathrm{IV}\right)}{\mathrm{AUC}}_{\left(0-t\right)\left(\mathrm{PO}\right)}\right)/\left({\mathrm{Dose}}_{\left(\mathrm{PO}\right)}{\mathrm{AUC}}_{\left(0-t\right)\left(\mathrm{IV}\right)}\right)100\%$

3. Experimental Results

[0274] The pharmacokinetic parameters of SD rats obtained for each of the test compounds based on the above method were shown in Table 13. The compounds of the present invention have better pharmacokinetic parameters, higher bioavailability than those of colchicine.

TABLE-US-00013 TABLE 13 Pharmacokinetic parameters of SD rats given each compound orally or intravenously Gavage - 10 mg/kg Compound t.sub.1/2 T.sub.max C.sub.max AUC.sub.INF F* MRT.sub.INF No. (h) (h) (ng/mL) (hr*ng/mL) (%) (h) colchicine / 0.417 175 735 8.73 25.7 48 1.61 0.25 1933 3305 57.0 1.83 55 2.02 0.333 2043 6257 115 2.72 56 1.27 0.417 1640 3716 62.3 2.20 58 2.22 2.67 2043 15113 109 4.58 122 1.79 0.250 1153 3129 50.1 2.47 124 1.38 0.333 2257 3379 44.7 1.71 125 3.33 0.25 4153 5459 93.0 1.8 132 1.39 0.25 1783 3335 47.5 2.10 Intravenous injection - 1 mg/kg Compound t.sub.1/2 AUC.sub.INF V.sub.SS CL MRT.sub.INF No. (h) (hr*ng/mL) (L/kg) (L/hr/kg) (h) colchicine / 567 17.6 1.91 11.0 48 0.536 580 1.11 1.75 0.641 55 0.6 543 1.10 1.87 0.592 56 0.590 597 0.977 1.72 0.582 58 1.92 1388 1.44 0.728 2.04 122 1.57 624 1.99 1.63 1.34 124 0.644 756 0.864 1.34 0.652 125 1.19 587 1.15 1.71 0.675 132 2.12 702 1.39 1.46 1.06 *Calculated by AUC.sub.INF

[0275] The foregoing are only better specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto, and any equivalent substitution or change made by a person skilled in the art familiar with the technical field of the present invention in accordance with the technical solution of the invention and its inventive concept shall be covered by the scope of protection of the invention.