Triterpenoids and compositions containing the same

Abstract

The present invention provides triterpenoids produced from natural compounds such as oleanolic acid, ursolic acid, betulinic acid, and hederagenin.

Claims

1. A compound having the sdstructure of Formula II: ##STR00028## wherein, at least one of X.sup.1 and X.sup.2 is OR.sup.a, NR.sup.aR.sup.b, or SR.sup.a, and the other of X.sup.1 and X.sup.2 is hydrogen, OR.sup.a, NR.sup.aR.sup.b, or SR.sup.a , wherein R.sup.a is a hydrogen, cyano, CF.sub.3, nitro, amino, or substituted or unsubstituted heteroaryl group; R.sup.b is hydrogen, hydroxyl, alkyl, aryl, aralkyl, acyl, alkoxy, aryloxy, acyloxy, alkylamino, arylamino, amido, or a substituted version of any of these groups; provided that R.sup.a is absent when the atom to which it is bound is part of a double bond, further provided that when R.sup.a is absent the atom to which it is bound is part of a double bond; Y is CH.sub.2 or CH.sub.2CH.sub.2; Z is a covalent bond, C(O), alkanediyl, alkenediyl, alkynediyl, or a substituted version of any of these groups; the dashed bonds can be independently present or absent; R.sup.1, R.sup.2, R.sup.3 and R.sup.4 are each independently a hydrogen, hydroxyl, alkyl, substituted alkyl, alkoxy or substituted alkoxy group; at least one of R.sup.5, R.sup.6, R.sup.8, R.sup.9 or R.sup.10 is independently OMs, CH.sub.2OMs, C(O)CCR.sup.a, CCCO.sub.2R.sup.a, CCSO.sub.2R.sup.a, CCC(O)R.sup.a or SO.sub.2R.sup.a, or R.sup.5 and R.sup.6, or R.sup.9 and R.sup.10are together CR.sup.cR.sup.d, wherein R.sup.c is hydrogen or alkylthiyl, and R.sup.d is hydrogen, halo, alkylthiyl, or substituted or unsubstituted alkylsulfonyl or alkylsulfonyl-O; the remainder of R.sup.5, R.sup.6, R.sup.8, R.sup.9 and R.sup.10 are independently hydrogen, hydroxyl, halo, cyano, O, CCR.sup.a, CO.sub.2R.sup.a, COR.sup.a, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl, acyl, alkoxy, aryloxy, acyloxy, alkylamino, arylamino, nitro, amino, amido, C(O)R.sup.e or a substituted version of any of these groups, wherein R.sup.e is hydrogen, hydroxy, halo, amino, hydroxyamino, azido or mercapto; or C.sub.1-C.sub.15-alkyl, C.sub.2-C.sub.15-alkenyl, C.sub.2-C.sub.15-alkynyl, C.sub.6-C.sub.15-aryl, C.sub.7-C.sub.15-aralkyl, C.sub.1-C.sub.15-heteroaryl, C.sub.2-C.sub.15-heteroaralkyl, C.sub.1-C.sub.15-acyl, C.sub.1-C.sub.15-alkoxy, C.sub.2-C.sub.15-alkenyloxy, C.sub.2-C.sub.15-alkynyloxy, C.sub.6-C.sub.15-aryloxy, C.sub.7-C.sub.15-aralkyloxy, C.sub.1-C.sub.15-heteroaryloxy, C.sub.2-C.sub.15-heteroaralkyloxy, C.sub.1-C.sub.15-acyloxy, C.sub.1-C.sub.15-alkylamino, C.sub.2-C.sub.15-dialkylamino, C.sub.1-C.sub.15-alkoxyamino, C.sub.2-C.sub.15-alkenylamino, C.sub.2-C.sub.15-alkynylamino, C.sub.6-C.sub.15-arylamino, C.sub.7-C.sub.15-aralkylamino, C.sub.1-C.sub.15-heteroarylamino, C.sub.2-C.sub.15-heteroaralkylamino, C.sub.1-C.sub.15-alkylsulfonylamino, C.sub.1-C.sub.15-amido, C.sub.1-C.sub.15-alkylsilyloxy, or substituted versions of any of these groups; R.sup.11 and R.sup.12 are each independently hydrogen, hydroxyl, halo, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl, acyl, alkoxy, aryloxy, aralkoxy, heteroaryloxy, hetero-aralkoxy, acyloxy, alkylamino, dialkylamino, arylamino, aralkylamino, heteroarylamino, heteroaralkylamino, amido, or a substituted version of any of these groups, or R.sup.11 and R.sup.12 are taken together and are alkanediyl, alkenediyl, arenediyl, alkoxydiyl, alkenyloxydiyl, alkylaminodiyl, alkenylaminodiyl, or alkenylaminooxydiyl; R.sup.13 is hydrogen, hydroxy or oxo; R.sup.14 is hydrogen or hydroxyl; and R.sup.15 is a hydrogen, hydroxyl, NR.sup.fR.sup.g, cyano, halo, azido, phosphate, 1,3-dioxoisoindolin-2-yl, mercapto, silyl or COOH group, substituted or unsubstituted versions of C.sub.1-C.sub.15-alkyl, C.sub.2-C.sub.15-alkenyl, C.sub.2-C.sub.15-alkynyl, C.sub.6-C.sub.15-aryl, C.sub.7-C.sub.15-aralkyl, C.sub.1-C.sub.15-heteroaryl, C.sub.2-C.sub.15-heteroaralkyl, C.sub.1-C.sub.15-acyl, C.sub.1-C.sub.15-alkoxy, C.sub.2-C.sub.15-alkenyloxy, C.sub.2-C.sub.15-alkynyloxy, C.sub.6-C.sub.15-aryloxy, C.sub.7-C.sub.15-aralkyloxy, C.sub.1-C.sub.15-heteroaryloxy, C.sub.2-C.sub.15-heteroaralkyloxy, C.sub.1-C.sub.15-acyloxy, C.sub.1-C.sub.15-alkylamino, C.sub.2-C.sub.15-alkenylamino, C.sub.2-C.sub.15-alkynylamino, C.sub.6-C.sub.15-arylamino, C.sub.7-C.sub.15-aralkylamino, C.sub.1-C.sub.15-heteroarylamino, C.sub.2-C.sub.15-heteroaralkylamino, C.sub.1-C.sub.15-amido, C.sub.1-C.sub.15-alkylthio, C.sub.2-C.sub.15-alkenylthio, C.sub.2-C.sub.15-alkynylthio, C.sub.6-C.sub.15-arylthio, C.sub.7-C.sub.15-aralkylthio, C.sub.1-C.sub.15-heteroarylthio, C.sub.2-C.sub.15-heteroaralkylthio, C.sub.1-C.sub.15-acylthio, C.sub.1-C.sub.12-thioacyl, C.sub.1-C.sub.12-alkylsulfonyl, C.sub.2-C.sub.12-alkenylsulfonyl, C.sub.2-C.sub.12-alkynylsulfonyl, C.sub.6-C.sub.12-arylsulfonyl, C.sub.7-C.sub.12-aralkylsulfonyl, C.sub.1-C.sub.12-heteroarylsulfonyl, C.sub.1-C.sub.12-heteroaralkylsulfonyl, C.sub.1-C.sub.12-alkylsulfinyl, C.sub.2-C.sub.12-alkenylsulfinyl, C.sub.2-C.sub.12-alkynylsulfinyl, C.sub.6-C.sub.12-aryl sulfinyl, C.sub.7-C.sub.12-aralkylsulfinyl, C.sub.1-C.sub.12-heteroarylsulfinyl, C.sub.1-C.sub.12-heteroaralkylsulfinyl, C.sub.1-C.sub.12-alkylphosphonyl, C.sub.1-C.sub.12-alkylphosphate, C.sub.2-C.sub.12-dialkylphosphate, C.sub.1-C.sub.12-alkylammonium, C.sub.1-C.sub.12-alkylsulfonium, C.sub.1-C.sub.15-alkylsilyl, or a substituted version of any of these groups, a CO.sub.2Me, carbonyl imidazole, CO-D-Glu(OAc).sub.4, CONH.sub.2, CONHNH.sub.2, CONHCH.sub.2CF.sub.3, or C(O)-heteroaryl group, or Z and R.sup.15 form a three to seven-membered ring, wherein R.sup.f and R.sup.g are independently hydrogen, hydroxyl, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl, acyl, alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkoxy, heteroaryloxy, heteroaralkoxy, thioacyl, alkylsulfonyl, alkenylsulfonyl, alkynylsulfonyl, arylsulfonyl, aralkylsulfonyl, heteroarylsulfonyl, or heteroaralkylsulfonyl, or a substituted version of any of these groups; L is C(O), CC, CCN(R), C(O)N(R), or CCC(O), wherein R is hydrogen, or an alkyl, aryl, alkenyl, or alkynyl group, or L is CCRCC, wherein R is an alkyl, aryl, alkenyl, or alkynyl group.

2. A pharmaceutical composition comprising the compound of claim 1 in admixture with a pharmaceutically acceptable carrier.

Description

DETAILED DESCRIPTION OF THE INVENTION

(1) Triterpenoids, including CDDO-Me derivatives, have now been developed. The triterpenoids described herein can be used in the treatment of disease, especially inflammatory diseases. Compounds particularly embraced by this invention have the structure of Formula I, which includes hydrates, isomers, prodrugs or pharmaceutically acceptable salts of Formula I:

(2) ##STR00001##
wherein,

(3) at least one of X.sup.1 and X.sup.2 is OR.sup.a, NR.sup.aR.sup.b, or SR.sup.a, and the other of X.sup.1 and X.sup.2 is hydrogen, OR.sup.a, NR.sup.aR.sup.b, or SR.sup.a wherein R.sup.a is a hydrogen, cyano, CF.sub.3, nitro, amino, or substituted or unsubstituted heteroaryl group; R.sup.b is hydrogen, hydroxyl, alkyl, aryl, aralkyl, acyl, alkoxy, aryloxy, acyloxy, alkylamino, arylamino, amido, or a substituted version of any of these groups; or a substituent convertible in vivo to hydrogen; provided that R.sup.a is absent when the atom to which it is bound is part of a double bond, further provided that when R.sup.a is absent the atom to which it is bound is part of a double bond;

(4) Y is CH.sub.2 or CH.sub.2CH.sub.2;

(5) Z is a covalent bond, C(O), alkanediyl, alkenediyl, alkynediyl, or a substituted version of any of these groups;

(6) the dashed bonds can be independently present or absent;

(7) R.sup.1, R.sup.2, R.sup.3 and R.sup.4 are each independently a hydrogen, hydroxyl, alkyl, substituted alkyl, alkoxy or substituted alkoxy group;

(8) at least one of R.sup.5, R.sup.6, R.sup.7, R.sup.8, R.sup.9 or R.sup.10 is independently OMs, CH.sub.2OMs, C(O)CCR.sup.a, CCCO.sub.2R.sup.a, CCSO.sub.2R.sup.a, CCC(O)R.sup.a or SO.sub.2R.sup.a, or

(9) R.sup.5 and R.sup.6, or R.sup.7 and R.sup.8, or R.sup.9 and R.sup.10 are together or CR.sup.cR.sup.d, wherein R.sup.c is hydrogen or alkylthiyl, and R.sup.d is hydrogen, halo, alkylthiyl, or substituted or unsubstituted alkylsulfonyl or alkylsulfonyl O;

(10) the remainder of R.sup.5, R.sup.6, R.sup.7, R.sup.8, R.sup.9 and R.sup.10 are independently hydrogen, hydroxyl, halo, cyano, O, CCR.sup.a, CO.sub.2R.sup.a, COR.sup.a, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl, acyl, alkoxy, aryloxy, acyloxy, alkylamino, arylamino, nitro, amino, amido, C(O)R.sup.e or a substituted version of any of these groups, wherein R.sup.e is hydrogen, hydroxy, halo, amino, hydroxyamino, azido or mercapto; or C.sub.1-C.sub.15-alkyl, C.sub.2-C.sub.15-alkenyl, C.sub.2-C.sub.15-alkynyl, C.sub.6-C.sub.15-aryl, C.sub.7-C.sub.15-aralkyl, C.sub.1-C.sub.15-heteroaryl, C.sub.2-C.sub.15-heteroaralkyl, C.sub.1-C.sub.15-acyl, C.sub.1-C.sub.15-alkoxy, C.sub.2-C.sub.15-alkenyloxy, C.sub.2-C.sub.15-alkynyloxy, C.sub.6-C.sub.15-aryloxy, C.sub.7-C.sub.15-aralkyloxy, C.sub.1-C.sub.15-heteroaryloxy, C.sub.2-C.sub.15-heteroaralkyloxy, C.sub.1-C.sub.15-acyloxy, C.sub.1-C.sub.15-alkylamino, C.sub.2-C.sub.15-dialkylamino, C.sub.1-C.sub.15-alkoxyamino, C.sub.2-C.sub.15-alkenylamino, C.sub.2-C.sub.15-alkynylamino, C.sub.6-C.sub.15-arylamino, C.sub.7-C.sub.15-aralkylamino, C.sub.1-C.sub.15-heteroarylamino, C.sub.2-C.sub.15-heteroaralkylamino, C.sub.1-C.sub.15-alkylsulfonylamino, C.sub.1-C.sub.15-amido, C.sub.1-C.sub.15-alkylsilyloxy, or substituted versions of any of these groups;

(11) R.sup.11 and R.sup.12 are each independently hydrogen, hydroxyl, halo, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl, acyl, alkoxy, aryloxy, aralkoxy, heteroaryloxy, hetero-aralkoxy, acyloxy, alkylamino, dialkylamino, arylamino, aralkylamino, heteroarylamino, heteroaralkylamino, amido, or a substituted version of any of these groups, or

(12) R.sup.11 and R.sup.12 are taken together and are alkanediyl, alkenediyl, arenediyl, alkoxydiyl, alkenyloxydiyl, alkylaminodiyl, alkenylaminodiyl, or alkenylaminooxydiyl;

(13) R.sup.13 is hydrogen, hydroxy or oxo;

(14) R.sup.14 is hydrogen or hydroxyl; and

(15) R.sup.15 is a hydrogen, hydroxyl, NR.sup.fR.sup.g, cyano, halo, azido, phosphate, 1,3-dioxoisoindolin-2-yl, mercapto, silyl or COOH group, substituted or unsubstituted versions of C.sub.1-C.sub.15-alkyl, C.sub.2-C.sub.15-alkenyl, C.sub.2-C.sub.15-alkynyl, C.sub.6-C.sub.15-aryl, C.sub.7-C.sub.15-aralkyl, C.sub.1-C.sub.15-heteroaryl, C.sub.2-C.sub.15-heteroaralkyl, C.sub.1-C.sub.15-acyl, C.sub.1-C.sub.15-alkoxy, C.sub.2-C.sub.15-alkenyloxy, C.sub.2-C.sub.15-alkynyloxy, C.sub.6-C.sub.15-aryloxy, C.sub.7-C.sub.15-aralkyloxy, C.sub.1-C.sub.15-heteroaryloxy, C.sub.2-C.sub.15-heteroaralkyloxy, C.sub.1-C.sub.15-acyloxy, C.sub.1-C.sub.15-alkylamino, C.sub.2-C.sub.15-alkenylamino, C.sub.2-C.sub.15-alkynylamino, C.sub.6-C.sub.15-arylamino, C.sub.7-C.sub.15-aralkylamino, C.sub.1-C.sub.15-heteroarylamino, C.sub.2-C.sub.15-heteroaralkylamino, C.sub.1-C.sub.15-amido, C.sub.1-C.sub.15-alkylthio, C.sub.2-C.sub.15-alkenylthio, C.sub.2-C.sub.15-alkynylthio, C.sub.6-C.sub.15-arylthio, C.sub.7-C.sub.15-aralkylthio, C.sub.1-C.sub.15-heteroarylthio, C.sub.2-C.sub.15-heteroaralkylthio, C.sub.1-C.sub.15-acylthio, C.sub.1-C.sub.12-thioacyl, C.sub.1-C.sub.12-alkylsulfonyl, C.sub.2-C.sub.12-alkenylsulfonyl, C.sub.2-C.sub.12-alkynylsulfonyl, C.sub.6-C.sub.12-arylsulfonyl, C.sub.7-C.sub.12-aralkylsulfonyl, C.sub.1-C.sub.12-heteroarylsulfonyl, C.sub.1-C.sub.12-heteroaralkylsulfonyl, C.sub.1-C.sub.12-alkylsulfinyl, C.sub.2-C.sub.12-alkenylsulfinyl, C.sub.2-C.sub.12-alkynylsulfinyl, C.sub.6-C.sub.12-aryl sulfinyl, C.sub.7-C.sub.12-aralkylsulfinyl, C.sub.1-C.sub.12-heteroarylsulfinyl, C.sub.1-C.sub.12-heteroaralkylsulfinyl, C.sub.1-C.sub.12-alkylphosphonyl, C.sub.1-C.sub.12-alkylphosphate, C.sub.2-C.sub.12-dialkylphosphate, C.sub.1-C.sub.12-alkylammonium, C.sub.1-C.sub.12-alkylsulfonium, C.sub.1-C.sub.15-alkylsilyl, or a substituted version of any of these groups, a CO.sub.2Me, carbonyl imidazole, CO-D-Glu(OAc).sub.4, CONH.sub.2, CONHNH.sub.2, CONHCH.sub.2CF.sub.3, or C(O)-heteroaryl group, or Z and R.sup.15 form a three to seven-membered ring, such that Z and R.sup.15 are further connected to one another through one or more of O and alkanediyl, further wherein Z is CH and R.sup.15 is CH.sub.2 or Z, R.sup.15, and carbon numbers 13, 17 and 18 form a ring such that R.sup.15 is bound to carbon 13, wherein Y is methanediyl or substituted methanediyl and R.sup.15 is O, wherein R.sup.f and R.sup.g are independently hydrogen, hydroxyl, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl, acyl, alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkoxy, heteroaryloxy, heteroaralkoxy, thioacyl, alkylsulfonyl, alkenylsulfonyl, alkynylsulfonyl, arylsulfonyl, aralkylsulfonyl, heteroarylsulfonyl, or heteroaralkylsulfonyl, or a substituted version of any of these groups.

(16) In certain embodiments, the bond between C.sub.2 and C.sub.3 in the A-ring is a double bond. In other embodiments, the bond between C.sub.2 and C.sub.3 in the A-ring is a single bond.

(17) In some embodiments, the compound of the invention is a dimer as represented by the Formula II,

(18) ##STR00002##
wherein X.sup.1, X.sup.2, Y, Z, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.8, R.sup.9, R.sup.11, R.sup.12, R.sup.13, R.sup.14 and R.sup.15 are as defined for Formula I and L is CCRCC, C(O), CC, CCN(R), C(O)N(R), CCC(O), ArC(O), or CCC(O)Ar, wherein R is hydrogen, or an alkyl, aryl, alkenyl, or alkynyl group. Exemplary dimers include compounds 10-17.

(19) In yet other embodiments, the compound of the invention has the structure as set forth in Formulae VI-XVI. In particular embodiments, the triterpenoid compound of the invention is a compound selected from compound 18-75.

(20) As used herein, hydrogen means H; hydroxyl means OH; oxo means O; halo or halogen means independently F, Cl, Br or I; hydroxyamino means NHOH; nitro means NO.sub.2; cyano means CN; azido means N.sub.3; mercapto means SH; thio means S; sulfonyl means S(O).sub.2 (see additional definitions of groups containing the term sulfonyl, e.g., alkylsulfonyl); and silyl means SiH.sub.3 (see additional definitions of group(s) containing the term silyl, e.g., alkylsilyl).

(21) For the groups below, the following parenthetical subscripts further define the groups as follows: (Cn) defines the exact number (n) of carbon atoms in the group. For example, C.sub.1-C.sub.15-alkoxy designates those alkoxy groups having from 1 to 15 carbon atoms (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc. or any range derivable therein (e.g., 3-10 carbon atoms)).

(22) The term alkyl refers to a non-aromatic monovalent group with a saturated carbon atom as the point of attachment, a linear or branched, cyclo, cyclic or acyclic structure, no carbon-carbon double or triple bonds, and no atoms other than carbon and hydrogen. The groups, CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, CH(CH.sub.2).sub.2, CH.sub.2CH.sub.2CH.sub.2CH.sub.3, CH(CH.sub.3)CH.sub.2CH.sub.3, CH.sub.2CH(CH.sub.3).sub.2, C(CH.sub.3).sub.3, CH.sub.2C(CH.sub.3).sub.3, cyclobutyl, cyclopentyl, cyclohexyl, and cyclohexylmethyl are non-limiting examples of alkyl groups.

(23) The term alkanediyl refers to a non-aromatic divalent group, wherein the alkanediyl group is attached with two -bonds, with one or two saturated carbon atom(s) as the point(s) of attachment, a linear or branched, cyclo, cyclic or acyclic structure, no carbon-carbon double or triple bonds, and no atoms other than carbon and hydrogen. The groups, CH.sub.2 (methylene), CH.sub.2CH.sub.2, CH.sub.2C(CH.sub.3).sub.2CH.sub.2, and CH.sub.2CH.sub.2CH.sub.2 are non-limiting examples of alkanediyl groups.

(24) The term alkenyl refers to a monovalent group with a nonaromatic carbon atom as the point of attachment, a linear or branched, cyclo, cyclic or acyclic structure, at least one nonaromatic carbon-carbon double bond, no carbon-carbon triple bonds, and no atoms other than carbon and hydrogen. Non-limiting examples of alkenyl groups include: CHCH.sub.2, CHCHCH.sub.3, CHCHCH.sub.2CH.sub.3, CH.sub.2CHCH.sub.2, CH.sub.2CHCHCH.sub.3, and CHCHC.sub.6H.sub.5.

(25) The term alkenediyl refers to a nonaromatic divalent group, wherein the alkenediyl group is attached with two -bonds, with two carbon atoms as points of attachment, a linear or branched, cyclo, cyclic or acyclic structure, at least one nonaromatic carbon-carbon double bond, no carbon-carbon triple bonds, and no atoms other than carbon and hydrogen. The groups, CHCH, CHC(CH.sub.3)CH.sub.2, and CHCHCH.sub.2 are non-limiting examples of alkenediyl groups.

(26) The term alkynyl refers to a monovalent group with a nonaromatic carbon atom as the point of attachment, a linear or branched, cyclo, cyclic or acyclic structure, at least one carbon-carbon triple bond, and no atoms other than carbon and hydrogen. The groups, CCH, CCCH.sub.3, CCC.sub.6H.sub.5 and CH.sub.2CCCH.sub.3, are non-limiting examples of alkynyl groups.

(27) The term alkynediyl refers to a nonaromatic divalent group, wherein the alkynediyl group is attached with two -bonds, with two carbon atoms as points of attachment, a linear or branched, cyclo, cyclic or acyclic structure, at least one carbon-carbon triple bond, and no atoms other than carbon and hydrogen. The groups, CC, CCCH.sub.2, and CCCH(CH.sub.3) are non-limiting examples of alkynediyl groups.

(28) The term aryl refers to a monovalent group with an aromatic carbon atom as the point of attachment, said carbon atom forming part of a six-membered aromatic ring structure wherein the ring atoms are all carbon, and wherein the monovalent group is composed of carbon and hydrogen. Non-limiting examples of aryl groups include phenyl, methylphenyl, (dimethyl)phenyl, -ethylphenyl, propylphenyl, C.sub.6H.sub.4CH(CH.sub.3).sub.2, C.sub.6H.sub.4CH(CH.sub.2).sub.2, methylethylphenyl, vinylphenyl, naphthyl, and the monovalent group derived from biphenyl.

(29) The term arenediyl refers to a divalent group, wherein the arenediyl group is attached with two -bonds, with two aromatic carbon atoms as points of attachment, said carbon atoms forming part of one or more six-membered aromatic ring structure(s) wherein the ring atoms are all carbon, and wherein the monovalent group is composed of carbon and hydrogen. Non-limiting examples of arenediyl groups include:

(30) ##STR00003##

(31) The term aralkyl refers to the monovalent group -alkanediyl-aryl, in which the terms alkanediyl and aryl are each used in a manner consistent with the definitions provided above. Non-limiting examples of aralkyls include 1-phenyl-ethyl, 2-phenyl-ethyl, indenyl and 2,3-dihydro-indenyl, provided that indenyl and 2,3-dihydro-indenyl are only examples of aralkyl in so far as the point of attachment in each case is one of the saturated carbon atoms.

(32) The term heteroaryl refers to a monovalent group with an aromatic carbon atom or nitrogen atom as the point of attachment, said carbon atom or nitrogen atom forming part of an aromatic ring structure wherein at least one of the ring atoms is nitrogen, oxygen or sulfur, and wherein the monovalent group is composed of carbon, hydrogen, aromatic nitrogen, aromatic oxygen or aromatic sulfur. Non-limiting examples of aryl groups include acridinyl, furanyl, imidazoimidazolyl, imidazopyrazolyl, imidazopyridinyl, imidazopyrimidinyl, indolyl, indazolinyl, methylpyridyl, oxazolyl, phenylimidazolyl, pyridyl, pyrrolyl, pyrimidyl, pyrazinyl, quinolyl, quinazolyl, quinoxalinyl, tetrahydroquinolinyl, thienyl, triazinyl, pyrrolopyridinyl, pyrrolopyrimidinyl, pyrrolopyrazinyl, pyrrolotriazinyl, pyrroloimidazolyl, chromenyl (where the point of attachment is one of the aromatic atoms), and chromanyl (where the point of attachment is one of the aromatic atoms).

(33) The term heteroaralkyl refers to the monovalent group -alkanediyl-heteroaryl, in which the terms alkanediyl and heteroaryl are each used in a manner consistent with the definitions provided above. Non-limiting examples of aralkyls include pyridylmethyl, and thienylmethyl.

(34) The term acyl refers to a monovalent group with a carbon atom of a carbonyl group as the point of attachment, further having a linear or branched, cyclo, cyclic or acyclic structure. The groups, CHO, C(O)CH.sub.3, C(O)CH.sub.2CH.sub.3, C(O)CH.sub.2CH.sub.2CH.sub.3, C(O)CH(CH.sub.3).sub.2, C(O)CH(CH.sub.2).sub.2, C(O)C.sub.6H.sub.5, C(O)C.sub.6H.sub.4CH.sub.3, and C(O)C.sub.6H.sub.4CH.sub.2CH.sub.3 are non-limiting examples of acyl groups. The term acyl therefore encompasses, but is not limited to groups sometimes referred to as alkyl carbonyl and aryl carbonyl groups.

(35) The term alkoxy refers to the group OR, in which R is an alkyl, as that term is defined herein. Non-limiting examples of alkoxy groups include OCH.sub.3, OCH.sub.2CH.sub.3, OCH.sub.2CH.sub.2CH.sub.3, OCH(CH.sub.3).sub.2, OCH(CH.sub.2).sub.2, O-cyclopentyl, and O-cyclohexyl.

(36) Similarly, the terms alkenyloxy, alkynyloxy, aryloxy, aralkoxy, heteroaryloxy, heteroaralkoxy and acyloxy, refer to groups, defined as OR, in which R is alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl and acyl, respectively, as those terms are defined above.

(37) The term alkoxydiyl refers to a non-aromatic divalent group, wherein the alkoxydiyl group is attached with two -bonds, with (a) two saturated carbon atoms as points of attachment, (b) one saturated carbon atom and one oxygen atom as points of attachment, or (c) two oxygen atoms as points of attachment, further having a linear or branched, cyclo, cyclic or acyclic structure, no carbon-carbon double or triple bonds in the group's backbone, further having no backbone atoms other than carbon or oxygen and having at least one of each of these atoms in the group's backbone. The groups, OCH.sub.2CH.sub.2, CH.sub.2OCH.sub.2CH.sub.2, OCH.sub.2CH.sub.2O and OCH.sub.2O are non-limiting examples of alkoxydiyl groups.

(38) The term alkenyloxydiyl refers to a divalent group that is nonaromatic prior to attachment, wherein the alkenyloxydiyl group is attached with two -bonds, which may become aromatic upon attachment, with (a) two carbon atoms as points of attachment, (b) one carbon atom and one oxygen atom as points of attachment, or (c) two oxygen atoms as points of attachment, further having a linear or branched, cyclo, cyclic or acyclic structure, at least one carbon-carbon double bond that is non-aromatic at least prior to attachment, further having no backbone atoms other than carbon or oxygen and having at least one of each of these atoms in the group's backbone. The groups, OCHCH, OCHCHO and OCHCHCH.sub.2 are non-limiting examples of alkenyloxydiyl groups.

(39) The term amino refers to a moiety of the formula NRR, wherein R and R are independently hydrogen, alkyl, cycloalkyl, heterocycloalkyl, aryl or heteroaryl.

(40) The term alkylamino refers to the group NHR, in which R is an alkyl, as that term is defined above. Non-limiting examples of alkylamino groups include NHCH.sub.3, NHCH.sub.2CH.sub.3, NHCH.sub.2CH.sub.2CH.sub.3, NHCH(CH.sub.3).sub.2, NHCH(CH.sub.2).sub.2, NHCH.sub.2CH.sub.2CH.sub.2CH.sub.3, NHCH(CH.sub.3)CH.sub.2CH.sub.3, NHCH.sub.2CH(CH.sub.3).sub.2, NHC(CH.sub.3).sub.3, NH-cyclopentyl, and NH-cyclohexyl.

(41) Similarly, the terms alkoxyamino, alkenylamino, alkynylamino, arylamino, aralkylamino, heteroarylamino, heteroaralkylamino, and alkylsulfonylamino refer to groups, defined as NHR, in which R is alkoxy, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl and alkylsulfonyl, respectively, as those terms are defined above. A non-limiting example of an arylamino group is NHC.sub.6H.sub.5.

(42) The term dialkylamino refers to the group NRR, in which R and R can be the same or different alkyl groups, or R and R can be taken together to represent an alkanediyl having two or more saturated carbon atoms, at least two of which are attached to the nitrogen atom. Non-limiting examples of dialkylamino groups include NHC(CH.sub.3).sub.3, N(CH.sub.3)CH.sub.2CH.sub.3, N(CH.sub.2CH.sub.3).sub.2, N-pyrrolidinyl, and N-piperidinyl.

(43) The term alkylaminodiyl refers to a non-aromatic divalent group, wherein the alkylaminodiyl group is attached with two -bonds, with (a) two saturated carbon atoms as points of attachment, (b) one saturated carbon atom and one nitrogen atom as points of attachment, or (c) two nitrogen atoms as points of attachment, further having a linear or branched, cyclo, cyclic or acyclic structure, no double or triple bonds in the group's backbone, further having no backbone atoms other than carbon or nitrogen and having at least one of each of these atoms in the group's backbone. The groups, NHCH.sub.2CH.sub.2, CH.sub.2NHCH.sub.2CH.sub.2, NHCH.sub.2CH.sub.2NH and NHCH.sub.2NH are non-limiting examples of alkylaminodiyl groups.

(44) The term alkenylaminodiyl refers to a divalent group that is nonaromatic prior to attachment, wherein the alkenylaminodiyl group is attached with two -bonds, which may become aromatic upon attachment, with (a) two carbon atoms as points of attachment, (b) one carbon atom and one nitrogen atom as points of attachment, or (c) two nitrogen atoms as points of attachment, further having a linear or branched, cyclo, cyclic or acyclic structure, at least one carbon-carbon double bond or carbon-nitrogen double that is non-aromatic at least prior to attachment, further having no backbone atoms other than carbon or nitrogen. The groups NHCHCH, NHCHN and NHCHCHNH are non-limiting examples of alkenylaminodiyl groups.

(45) The term alkenylaminooxydiyl refers to a divalent group, wherein the alkenylaminooxydiyl group is attached with two -bonds, which may become aromatic upon attachment, with two atoms selected from the group consisting of carbon, oxygen and nitrogen as points of attachment, further having a linear or branched, cyclo, cyclic or acyclic structure, at least one carbon-carbon double bond, carbon-nitrogen double, or nitrogen-nitrogen double bond that is non-aromatic at least prior to attachment, further having no backbone atoms other than carbon nitrogen or oxygen and having at least one of each of these three atoms in the backbone. The group OCHN, is a non-limiting example of an alkenylaminooxydiyl group.

(46) The term amido (acylamino) refers to the group NHR, in which R is acyl, as that term is defined herein. A non-limiting example of an acylamino group is NHC(O)CH.sub.3.

(47) The term alkylthio refers to the group SR, in which R is an alkyl, as that term is defined above. Non-limiting examples of alkylthio groups include SCH.sub.3, SCH.sub.2CH.sub.3, SCH.sub.2CH.sub.2CH.sub.3, SCH(CH.sub.3).sub.2, SCH(CH.sub.2).sub.2, S-cyclopentyl, and S-cyclohexyl.

(48) Similarly, the terms alkenylthio, alkynylthio, arylthio, aralkylthio, heteroarylthio, heteroaralkylthio and acylthio refer to groups, defined as SR, in which R is alkenyl, alkynyl, aryl, aralkyl, heteroaryl, heteroaralkyl and acyl, respectively, as those terms are defined above.

(49) The term thioacyl refers to a monovalent group with a carbon atom of a thiocarbonyl group as the point of attachment, further having a linear or branched, cyclo, cyclic or acyclic structure. The groups CHS, C(S)CH.sub.3, C(S)CH.sub.2CH.sub.3, C(S)CH.sub.2CH.sub.2CH.sub.3, C(S)CH(CH.sub.3).sub.2, C(S)CH(CH.sub.2).sub.2, C(S)C.sub.6H.sub.5, C(S)C.sub.6H.sub.4CH.sub.3, C(S)C.sub.6H.sub.4CH.sub.2CH.sub.3, C(S)C.sub.6H.sub.3(CH.sub.3).sub.2, and C(S)CH.sub.2C.sub.6Hs, are non-limiting examples of thioacyl groups. The term thioacyl therefore encompasses, but is not limited to, groups sometimes referred to as alkyl thiocarbonyl and aryl thiocarbonyl groups.

(50) The term alkylsulfonyl refers to the group S(O).sub.2R, in which R is an alkyl, as that term is defined above. Non-limiting examples of alkylsulfonyl groups include: S(O).sub.2CH.sub.3, S(O).sub.2CH.sub.2CH.sub.3, S(O).sub.2CH.sub.2CH.sub.2CH.sub.3, S(O).sub.2CH(CH.sub.3).sub.2, S(O).sub.2CH(CH.sub.2).sub.2, S(O).sub.2-cyclopentyl, and S(O).sub.2-cyclohexyl.

(51) Similarly, the terms alkenylsulfonyl, alkynylsulfonyl, arylsulfonyl, aralkylsulfonyl, heteroarylsulfonyl, and heteroaralkylsulfonyl refer to groups, defined as S(O).sub.2R, in which R is alkenyl, alkynyl, aryl, aralkyl, heteroaryl, and heteroaralkyl, respectively, as those terms are defined above.

(52) The term alkylsulfinyl refers to the group S(O)R, in which R is an alkyl, as that term is defined above. Non-limiting examples of alkylsulfinyl groups include S(O)CH.sub.3, S(O)CH.sub.2CH.sub.3, S(O)CH.sub.2CH.sub.2CH.sub.3, S(O)CH(CH.sub.3).sub.2, S(O)CH(CH.sub.2).sub.2, S(O)-cyclopentyl, and S(O)-cyclohexyl.

(53) Similarly, the terms alkenylsulfinyl, alkynylsulfinyl, arylsulfinyl, aralkylsulfinyl, heteroarylsulfinyl and heteroaralkylsulfinyl refer to groups, defined as S(O)R, in which R is alkenyl, alkynyl, aryl, aralkyl, heteroaryl, and heteroaralkyl, respectively, as those terms are defined above.

(54) The term alkylammonium refers to a group, defined as NH.sub.2R.sup.+, NHRR.sup.+, or NRRR.sup.+, in which R, R and R are the same or different alkyl groups, or any combination of two of R, R and R can be taken together to represent an alkanediyl. Non-limiting examples of alkylammonium cation groups include NH.sub.2(CH.sub.3).sup.+, NH.sub.2(CH.sub.2CH.sub.3)+, NH.sub.2(CH.sub.2CH.sub.2CH.sub.3)+, NH(CH.sub.3).sub.2.sup.+, NH(CH.sub.2CH.sub.3).sub.2.sup.+, NH(CH.sub.2CH.sub.2CH.sub.3).sub.2.sup.+, N(CH.sub.3).sub.3.sup.+, N(CH.sub.3)(CH.sub.2CH.sub.3).sub.2.sup.+, N(CH.sub.3).sub.2(CH.sub.2CH.sub.3).sup.+, NH.sub.2C(CH.sub.3).sub.3.sup.+, NH(cyclopentyl).sub.2.sup.+, and NH.sub.2(cyclohexyl).sup.+.

(55) The term alkylthiyl refers to the group SR. Non-limiting examples of alkylthiyl groups include S(CH.sub.3), S(CH.sub.2CH.sub.3), S(CH.sub.2CH.sub.2CH.sub.3)S(cyclopentyl), and S(cyclohexyl).

(56) The term alkylsilyl refers to a monovalent group, defined as SiH.sub.2R, SiHRR, or SiRRR, in which R, R and R can be the same or different alkyl groups, or any combination of two of R, R and R can be taken together to represent an alkanediyl. The groups SiH.sub.2CH.sub.3, SiH(CH.sub.3).sub.2, Si(CH.sub.3).sub.3 and Si(CH.sub.3).sub.2C(CH.sub.3).sub.3, are non-limiting examples of unsubstituted alkylsilyl groups.

(57) The term alkylphosphonyl refers to the group OPO(OR).sub.2, where R is alkyl, as defined herein.

(58) The term alkylphosphate refers to the group OP(O)(OH)(OR), in which R is an alkyl, as that term is defined above. Non-limiting examples of alkylphosphate groups include OP(O)(OH)(OMe) and OP(O)(OH)(OEt).

(59) The term dialkylphosphate refers to the group OP(O)(OR)(OR), in which R and R can be the same or different alkyl groups, or R and R can be taken together to represent an alkanediyl having two or more saturated carbon atoms, at least two of which are attached via the oxygen atoms to the phosphorus atom. Non-limiting examples of dialkylphosphate groups include OP(O)(OMe).sub.2, OP(O)(OEt)(OMe) and OP(O)(OEt).sub.2.

(60) Cycloalkyl means a non-aromatic mono- or multicyclic ring system including about 3 to about 10 carbon atoms, preferably about 5 to about 10 carbon atoms. Non-limiting examples of suitable monocyclic cycloalkyls include cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl and the like. Non-limiting examples of suitable multicyclic cycloalkyls include 1-decalinyl, norbornyl, adamantyl and the like.

(61) Heterocyclyl or heterocycloalkyl means a non-aromatic saturated monocyclic or multicyclic ring system including about 3 to about 10 ring atoms, preferably about 5 to about 10 ring atoms, in which one or more of the atoms in the ring system is an element other than carbon, for example nitrogen, oxygen or sulfur, alone or in combination. Preferred heterocyclyls contain about 5 to about 6 ring atoms. The prefix aza, oxa or thia before the heterocyclyl root name means that at least a nitrogen, oxygen or sulfur atom respectively is present as a ring atom. The nitrogen or sulfur atom of the heterocyclyl can be optionally oxidized to the corresponding N-oxide, S-oxide or S,S-dioxide. Non-limiting examples of suitable monocyclic heterocyclyl rings include piperidyl, pyrrolidinyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, lactam, lactone, and the like. Non-limiting examples of suitable bicyclic heterocyclyl rings include decahydro-isoquinoline, decahydro-[2,6]naphthyridine, and the like.

(62) Any of the groups described herein may be unsubstituted or optionally substituted. When modifying a particular group, substituted means that the group the term modifies may, but does not have to, be substituted. Substitutions typically replace an available hydrogen with an alkyl, alkenyl, alkynyl, aryl, heteroaryl, aralkyl, alkylaryl, heteroaralkyl, heteroarylalkenyl, heteroarylalkynyl, alkylheteroaryl, hydroxy, hydroxyalkyl, alkoxy, aryloxy, aralkoxy, alkoxyalkoxy, acyl, halo, nitro, cyano, carboxy, alkoxycarbonyl, aryloxycarbonyl, aralkoxycarbonyl, alkylsulfonyl, arylsulfonyl, heteroarylsulfonyl, alkylthio, arylthio, heteroarylthio, aralkylthio, heteroaralkylthio, cycloalkyl, or heterocyclyl.

(63) Any undefined valency on an atom of a structure shown in this application implicitly represents a hydrogen atom bonded to the atom.

(64) The term hydrate when used as a modifier to a compound means that the compound has less than one (e.g., hemihydrate), one (e.g., monohydrate), or more than one (e.g., dihydrate) water molecules associated with each compound molecule, such as in solid forms of the compound.

(65) An isomer of a first compound is a separate compound in which each molecule contains the same constituent atoms as the first compound, but where the configuration of those atoms in three dimensions differs.

(66) Pharmaceutically acceptable salts means salts of compounds of the present invention which are pharmaceutically acceptable, and which possess the desired pharmacological activity. Such salts include acid addition salts formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or with organic acids such as 1,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, 2-naphthalenesulfonic acid, 3-phenylpropionic acid, 4,4-methylenebis(3-hydroxy-2-ene-1-carboxylic acid), 4-methylbicyclo[2.2.2]oct-2-ene-1-carboxylic acid, acetic acid, aliphatic mono- and di-carboxylic acids, aliphatic sulfuric acids, aromatic sulfuric acids, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, carbonic acid, cinnamic acid, citric acid, cyclopentanepropionic acid, ethanesulfonic acid, fumaric acid, glucoheptonic acid, gluconic acid, glutamic acid, glycolic acid, heptanoic acid, hexanoic acid, hydroxynaphthoic acid, lactic acid, laurylsulfuric acid, maleic acid, malic acid, malonic acid, mandelic acid, methanesulfonic acid, muconic acid, o-(4-hydroxybenzoyl)benzoic acid, oxalic acid, p-chlorobenzenesulfonic acid, phenyl-substituted alkanoic acids, propionic acid, p-toluenesulfonic acid, pyruvic acid, salicylic acid, stearic acid, succinic acid, tartaric acid, tertiarybutylacetic acid, trimethylacetic acid, and the like. Pharmaceutically acceptable salts also include base addition salts which may be formed when acidic protons present are capable of reacting with inorganic or organic bases. Acceptable inorganic bases include sodium hydroxide, sodium carbonate, potassium hydroxide, aluminum hydroxide and calcium hydroxide. Acceptable organic bases include ethanolamine, diethanolamine, triethanolamine, tromethamine, N-methylglucamine and the like. It should be recognized that the particular anion or cation forming a part of any salt of this invention is not critical, so long as the salt, as a whole, is pharmacologically acceptable. Additional examples of pharmaceutically acceptable salts and their methods of preparation and use are presented in Handbook of Pharmaceutical Salts: Properties, and Use (P. H. Stahl & C. G. Wermuth eds., Verlag Helvetica Chimica Acta, 2002).

(67) Compounds of the invention may also exist in prodrug form. Since prodrugs are known to enhance numerous desirable qualities of pharmaceuticals, e.g., solubility, bioavailability, manufacturing, etc., the compounds employed in some methods of the invention may, if desired, be delivered in prodrug form. Thus, the invention contemplates prodrugs of compounds of the present invention as well as methods of delivering prodrugs. Prodrugs of the compounds employed in the invention may be prepared by modifying functional groups present in the compound in such a way that the modifications are cleaved, either in routine manipulation or in vivo, to the parent compound. Accordingly, prodrugs include, for example, compounds described herein in which a hydroxy, amino, or carboxy group is bonded to any group that, when the prodrug is administered to a patient, cleaves to form a hydroxy, amino, or carboxylic acid, respectively. For example, a compound comprising a hydroxy group may be administered as an ester that is converted by hydrolysis in vivo to the hydroxy compound. Suitable esters that may be converted in vivo into hydroxy compounds include acetates, citrates, lactates, phosphates, tartrates, malonates, oxalates, salicylates, propionates, succinates, fumarates, maleates, methylene-bis--hydroxynaphthoate, gentisates, isethionates, di-p-toluoyltartrates, methanesulfonates, ethanesulfonates, benzenesulfonates, p-toluenesulfonates, cyclohexylsulfamates, quinates, esters of amino acids, and the like. Similarly, a compound comprising an amine group may be administered as an amide that is converted by hydrolysis in vivo to the amine compound.

(68) A triterpenoid compound of this invention may be administered in a pharmaceutical composition by various routes including, but not limited to, oral, subcutaneous, intravenous, or intraperitoneal administration (e.g. by injection). Depending on the route of administration, the active compound may be coated in a material to protect the compound from the action of acids and other natural conditions which may inactivate the compound.

(69) For example, to administer the therapeutic compound by other than parenteral administration, it may be necessary to coat the compound with, or co-administer the compound with, a material to prevent its inactivation. By way of illustration, the therapeutic compound may be administered to a subject in an appropriate carrier, for example, liposomes, or a diluent. Pharmaceutically acceptable diluents include saline and aqueous buffer solutions. Liposomes include water-in-oil-in-water CGF emulsions as well as conventional liposomes (Strejan, et al. (1984) J. Neuroimmunol. 7:27).

(70) The therapeutic compound may also be administered parenterally, intraperitoneally, intraspinally, or intracerebrally. Dispersions can be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations may contain a preservative to prevent the growth of microorganisms.

(71) Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. In all cases, the composition must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), suitable mixtures thereof, and vegetable oils. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, sodium chloride, or polyalcohols such as mannitol and sorbitol, in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate or gelatin.

(72) Sterile injectable solutions can be prepared by incorporating the therapeutic compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the therapeutic compound into a sterile carrier which contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient (i.e., the therapeutic compound) plus any additional desired ingredient from a previously sterile-filtered solution thereof.

(73) The therapeutic compound can be orally administered, for example, with an inert diluent or an assimilable edible carrier. The therapeutic compound and other ingredients may also be enclosed in a hard or soft shell gelatin capsule, compressed into tablets, or incorporated directly into the subject's diet. For oral therapeutic administration, the therapeutic compound may be incorporated with excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like. The percentage of the therapeutic compound in the compositions and preparations may, of course, be varied. The amount of the therapeutic compound in such therapeutically useful compositions is such that a suitable dosage will be obtained.

(74) It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit containing a predetermined quantity of therapeutic compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on (a) the unique characteristics of the therapeutic compound and the particular therapeutic effect to be achieved, and (b) the limitations inherent in the art of compounding such a therapeutic compound for the treatment of a selected condition in a subject.

(75) One or more triterpenoid compounds of the invention are administered at a therapeutically effective dosage sufficient to treat a condition in a subject. A therapeutically effective dosage preferably reduces the amount of symptoms of the condition in the infected subject by at least about 20%, more preferably by at least about 40%, even more preferably by at least about 60%, and still more preferably by at least about 80% relative to untreated subjects. For example, the efficacy of a compound can be evaluated in an animal model system that may be predictive of efficacy in treating the disease in humans

(76) The triterpenoid compounds of the invention are of use in modulating IFN--induced NO production in macrophages, said composition having an IC.sub.50 value of at least less than 0.6 M, more preferably less than 0.001 M.

(77) In one embodiment, the instant triterpenoid compounds are of use in a method of modulating excessive nitric oxide or prostaglandin formation in a subject by administering to a subject a pharmaceutically effective amount of one or more triterpenoid compounds, such that the nitric oxide or prostaglandin formation is modulated.

(78) In a further embodiment, the triterpenoid compounds of the invention are of use in a method of preventing or treating a disorder characterized by overexpression of iNOS or COX-2 genes, wherein the method includes administering to a subject a pharmaceutically effective amount of one or more triterpenoid compounds, such that the disorder is prevented or treated. In a preferred embodiment, the disorder includes cancer, diabetic nephropathy, neurodegenerative disease, rheumatoid arthritis, inflammatory bowel disease, and other diseases whose pathogenesis is believed to involve excessive production of either nitric oxide or prostaglandins. In a particular embodiment, the neurodegenerative disease includes Parkinson's disease, Alzheimer's disease, multiple sclerosis, and amyotrophic lateral sclerosis. The cancer may include, e.g., a leukemic cancer or a solid cancer. A leukemic cancer is a cancer of a blood cell, a myeloid cell, a monocytic cell, a myelocytic cell, a promyelocytic cell, a myeloblastic cell, a lymphocytic cell, or a lymphoblastic cell. A solid cancer is a cancer of a bladder cell, a breast cell, a lung cell, a colon cell, a prostate cell, a liver cell, a pancreatic cell, a stomach cell, a testicular cell, a brain cell, an ovarian cell, a skin cell, a brain cell, a bone cell, or a soft tissue cell.

(79) Moreover, the invention provides methods for the treatment and prevention of graft versus host disease (GVHD) by providing a triterpenoid compound of the invention either alone or in conjunction with another agent, such as an immunosuppressive agent such as a corticosteroid or tacrolimus, or a chemotherapeutic agent for the treatment of GVHD. In graft versus host disease the donor immune system mounts a response against the host's organs or tissue. As CDDO compounds, either alone or in conjunction with other agents, can induce apoptosis by inhibiting Bcl-2 and have activity in lymphoid tissue, it is contemplated that the instant triterpenoid compounds can be used to provide therapy for graft versus host diseases.

(80) The practice of the methods of the present invention will employ, unless otherwise indicated, conventional techniques of cell biology, cell culture, molecular biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See, for example, Genetics; Molecular Cloning A Laboratory Manual, 2nd Ed., ed. by Sambrook, J. et al. (1989) Cold Spring Harbor Laboratory Press; Short Protocols in Molecular Biology, 3rd Ed., ed. by Ausubel, F. et al. (1995) Wiley, NY; DNA Cloning, Volumes I and II (D. N. Glover ed., 1985); Oligonucleotide Synthesis (M. J. Gait ed. (1984)); Mullis et al. U.S. Pat. No. 4,683,195; Nucleic Acid Hybridization (B. D. Hames & S. J. Higgins eds. (1984)); the treatise, Methods In Enzymology (Academic Press, Inc., N.Y.); Immunochemical Methods In Cell And Molecular Biology (Mayer and Walker, eds., Academic Press, London (1987)); Handbook Of Experimental Immunology, Volumes I-IV (D. M. Weir and C. C. Blackwell, eds. (1986)); and Miller, J. Experiments in Molecular Genetics (Cold Spring Harbor Press, Cold Spring Harbor, N.Y. (1972)).

(81) The invention is described in greater detail by the following non-limiting examples.

Example 1

Synthesis of Triterpenoids

(82) The triterpenoids of the invention can be generally produced from natural compounds such as oleanolic acid, ursolic acid, betulinic acid, or hederagenin, or derivatives thereof that include additional A and/or C ring modifications. Synthesis of the compounds can be achieved using any conventional method of synthesizing similar triterpenoids such as CDDO or CDDO-Me. See, e.g., U.S. Pat. Nos. 6,326,507, 6,552,075, 6,974,801, 7,288,568, 7,863,327, 7,915,402 , 7,943,778, 8,034,955, 8,071,632, 8,124,656, 8,124,799, 8,129,429 and WO 2009/146216.

(83) As one example, triterpenoid compounds of the invention can be synthesized by (a) methylating the carboxylic acid group of a compound of Formula III to afford a methyl ester (Formula IV); oxidizing the hydroxyl group of a compound of Formula IV with an oxidizing agent to form a double bond in Ring A (Formula V), epoxidating Ring C of the enone to form an epoxide, and forming a C-ring enol and halogenating the A-ring enone to yield a compound of Formula VI (Scheme 1)

(84) ##STR00004##

(85) According to this example, the methylating agent is an electrophilic methyl source including, but not limited to iodomethane, dimethyl sulfate, dimethyl carbonate, diazomethane, or with methylating reagents such as methyl triflate or methyl fluorosulfonate, optionally in the presence of a base such as K.sub.2CO.sub.3 or Li.sub.2CO.sub.3. Further, the oxidizing agent can be an iodine oxidizing agent such as o-iodoxybenzoic acid (IBX)(Nicolaou, et al. (2002) J. Am. Chem. Soc. 124:2245-2258), diacetoxyiodobenzene (DAIB), fluorous DAIB (F-DAIB), Dess-Martin-Periodinane (DMP), or a stabilized formulation of IBX (SIBX; Ozanne, et al. (2003) Org. Lett. 5:2903) in one or a combination of suitable solvents such as DMSO and phenyl fluoride (fluorobenzene). Furthermore, epoxidation of Ring C can be carried out with an oxidant such as a peroxyacid, e.g., meta-chloroperoxybenzoic acid (mCPBA), peroxyacetic acid, or potassium peroxymonosulfate (Oxone). Acid catalyzed opening of the epoxide and bromination of the A Ring can be achieved with a hydrogen halide such as (HBr or HI) and a diatomic halogen molecule such as Br.sub.2 or I.sub.2.

(86) As used in the context of the present invention, Formula III includes naturally occurring starting materials such as oleanolic acid, ursolic acid, betulinic acid, or hederagenin, or derivatives thereof.

(87) ##STR00005##

(88) As a specific example of using this synthetic method in the synthesis of triterpenoids, CDDO-Me was synthesized from oleanolic acid. As shown in Scheme 2, the natural triterpenoid oleanolic acid (1) was used as the starting material in the synthesis of CDDO-Me. The method commences with methylation of the carboxylic acid of oleanolic acid (1) to afford methyl ester 2 in quantitative yield. With ester 2, activation of the A-ring is fulfilled by 2-iodoxybenzoic acid-mediated two-fold oxidation to give enone 3. Epoxidation with meta-chloroperoxybenzoic acid, followed by direct C-ring enolization and A-ring enone bromination with bromine and hydrobromic acid, affords key intermediate 4. With bromide 4 in hand, a cross-coupling reaction with copper cyanide provides CDDO-Me (5) (Scheme 2). Intermediate 4 was prepared in high yield and few overall steps, thereby providing a base compound for development of the analogs and derivatives described herein.

(89) ##STR00006##

(90) Unless otherwise specified, the reagents used in the instant methods are conventionally known in the art. For example, MeI refers to methyl iodide, DMF refers to dimethylformamide, IBX is 2-iodoxybenzoic acid, DMSO is dimethyl sulfoxide, PhF is phenyl fluoride, mCPBA refers to meta-chloroperoxybenzoic acid, HBr is hydrogen bromide, DCM is dichloromethane, AcOH is acetic acid, and CuCN is copper cyanide.

Example 2

Ring A Modifications

(91) Given the reactivity of Ring A halogen, Formula VI serves as a substrate for the synthesis of the triterpenoid derivatives of the invention. For example, contact of a compound of Formula VI with a cyanide ion source such as K.sub.4[Fe(CN).sub.6], KCN, NaCN, ZnCN, CuCN, (CH.sub.3).sub.2C(OH)CN or TMSCN results in the displacement of the aromatic halide with a cyanide ion. In other embodiments, the compound of Formula VI can be reacted with a wide variety of reagents to replace the halogen on Ring A. For example, the compound of Formula VI can be aminated or coupled or cross-coupled with an alkyl, alkenyl, alkynyl or aryl group to provide a variety of substituents on Ring A. For example, Formula VI can be aminated via Buchwald-Hartwig amination (Buchwald & Muci (2002) Top. Curr. Chem. 219:133-209; Hartwig (1999) Pure Appl. Chem. 71:1417; Buchwald & Yang (1999) J. Orgmet. Chem. 576:125; Hartwig (1998) ACIEE 37:2046; Hartwig (1998) Acc. Chem. Res. 31:852; Buchwald et al. (1998) Acc. Chem. Res. 31:805) to provide amides and amines 6 (R.sup.16=H and R.sup.17=H or CHO). Moreover, when 6 is a formamide (R.sup.16=H and R.sup.17=CHO), isonitrile 7 can be readily synthesized under mild conditions (Porcheddu, et al. (2005) J. Org. Chem. 70:2361-3). In addition, Sonogashira coupling (Sonogashira, et al. (1975) Tetrahedron Lett. 16:4467-70) provides alkynes 8 and 10. Likewise, Suzuki (Miyuara, et al. (1979) Tetrahedron Lett. 20:3437-40; Miyaura & Suzuki (1979) Chem. Comm. 19:866-7; Miyaura & Suzuki (1995) Chem. Rev. 95:2457-2483), Stille (Kosugi, et al. (1977) Chem. Lett. 301; Milstein & Stille (1978) J. Am. Chem. Soc. 100:3636), and Negishi (King, et al. (1977) J. Chem. Soc. Chem. Commun. 19:683) cross-coupling reactions provide compounds having the structure of compound 9 and dimers such as compounds 10-17 are readily produced when the halogen of Formula VI is iodide.

(92) ##STR00007## ##STR00008## ##STR00009##

Example 3

Derivatives with Modifications at C-17

(93) Amides (Formula VI), ethers (Formula VII), and esters (Formula VIII and Formula IX) are readily obtained using the instant method in combination with techniques known in the art. See U.S. Pat. No. 6,974,801 and US 2008/0233195.

(94) ##STR00010##

(95) In accordance with Formulae VI-IX,

(96) R.sup.11 and R.sup.12 are as defined for Formula I;

(97) R.sup.18 is OMs, CH.sub.2OMs, C(O)CCR.sup.a, CCCO.sub.2R.sup.a, CCSO.sub.2R.sup.a, CCC(O)R.sup.a, SO.sub.2R.sup.a, O or CR.sup.cR.sup.d, wherein

(98) R.sup.d is hydrogen, halo, alkylthiyl, or substituted or unsubstituted alkylsulfonyl or alkylsulfonyl O;

(99) R.sup.22 and R.sup.23 are independently a hydrogen, hydroxyl, halo, alkyl, nitro or amino group;

(100) R.sup.19, R.sup.20, R.sup.21, R.sup.24 and R.sup.25 are independently a hydrogen, hydroxyl, NR.sup.fR.sup.g, cyano, halo, azido, phosphate, 1,3-dioxoisoindolin-2-yl, mercapto, silyl or COOH group,

(101) substituted or unsubstituted versions of C.sub.1-C.sub.15-alkyl, C.sub.2-C.sub.15-alkenyl, C.sub.2-C.sub.15-alkynyl, C.sub.6-C.sub.15-aryl, C.sub.7-C.sub.15-aralkyl, C.sub.1-C.sub.15-heteroaryl, C.sub.2-C.sub.15-heteroaralkyl, C.sub.1-C.sub.15-acyl, C.sub.2-C.sub.15-alkenyloxy, C.sub.2-C.sub.15-alkynyloxy, C.sub.6-C.sub.15-aryloxy, C.sub.7-C.sub.15-aralkyloxy, C.sub.1-C.sub.15-heteroaryloxy, C.sub.2-C.sub.15-heteroaralkyloxy, C.sub.1-C.sub.15-acyloxy, C.sub.1-C.sub.15-alkylamino, C.sub.2-C.sub.15-alkenylamino, C.sub.2-C.sub.15-alkynylamino, C.sub.6-C.sub.15-arylamino, C.sub.7-C.sub.15-aralkylamino, C.sub.1-C.sub.15-heteroarylamino, C.sub.2-C.sub.15-heteroaralkylamino, C.sub.1-C.sub.15-amido, C.sub.1-C.sub.15-alkylthio, C.sub.2-C.sub.15-alkenylthio, C.sub.2-C.sub.15-alkynylthio, C.sub.6-C.sub.15-arylthio, C.sub.7-C.sub.15-aralkylthio, C.sub.1-C.sub.15-heteroarylthio, C.sub.2-C.sub.15-heteroaralkylthio, C.sub.1-C.sub.15-acylthio, C.sub.1-C.sub.12-thioacyl, C.sub.1-C.sub.12-alkylsulfonyl, C.sub.2-C.sub.12-alkenylsulfonyl, C.sub.2-C.sub.12-alkynylsulfonyl, C.sub.6-C.sub.12-arylsulfonyl, C.sub.7-C.sub.12-aralkylsulfonyl, C.sub.1-C.sub.12-heteroarylsulfonyl, C.sub.1-C.sub.12-heteroaralkylsulfonyl, C.sub.1-C.sub.12-alkylsulfinyl, C.sub.2-C.sub.12-alkenylsulfinyl, C.sub.2-C.sub.12-alkynylsulfinyl, C.sub.6-C.sub.12-arylsulfinyl, C.sub.7-C.sub.12-aralkylsulfinyl, C.sub.1-C.sub.12-heteroarylsulfinyl, C.sub.1-C.sub.12-heteroaralkylsulfinyl, C.sub.1-C.sub.12-alkylphosphonyl, C.sub.1-C.sub.12-alkylphosphate, C.sub.2-C.sub.12-dialkylphosphate, C.sub.1-C.sub.12-alkylammonium, C.sub.1-C.sub.12-alkylsulfonium, C.sub.1-C.sub.15-alkylsilyl, or a substituted version of any of these groups,

(102) a CO.sub.2Me, carbonyl imidazole, CO-D-Glu (OAc)CONH.sub.2, CONHNH.sub.2, CONHCH.sub.2CF.sub.3, or C(O)-heteroaryl group.

Example 4

CDDO-Me Derivatives with A-Ring Modifications

(103) CDDO-Me derivatives within the scope of Formula I and containing A-Ring modifications are as follows.

(104) ##STR00011##

(105) In accordance with Formulae X-XIII,

(106) R.sup.11 and R.sup.12 are as defined for Formula I;

(107) X is O or OMe;

(108) dashed bonds are present or absent;

(109) R.sup.26 and R.sup.27 are independently a hydrogen, halo (e.g., Cl or F), alkylthiyl, or substituted or unsubstituted alkylsulfonyl or alkylsulfonyl O;

(110) R.sup.28 and R.sup.29 are independently H, or together are O.

(111) Exemplary compounds of Formulae X-XIII include compounds 18-82:

(112) ##STR00012## ##STR00013## ##STR00014## ##STR00015## ##STR00016## ##STR00017## ##STR00018## ##STR00019## ##STR00020## ##STR00021##

Example 5

Mesylate Derivatives of CDDO-Me

(113) Mesylate derivatives of CDDO-Me of Formula XIV-XVI are also included with the scope of this invention.

(114) ##STR00022##

(115) In accordance with Formulae XIV-XVI:

(116) R.sup.11 and R.sup.12 are as defined for Formula I;

(117) R.sup.30, R.sup.31, R.sup.32 and R.sup.33 are each independently a hydrogen, cyano, OMs, or CH.sub.2OMs group; or

(118) R.sup.30 and R.sup.31 or R.sup.32 and R.sup.33 together are CH.sub.2; and

(119) dashed bonds are either present or absent.

(120) Exemplary compounds of Formulae XIV-XVI include compounds 62-75:

(121) ##STR00023## ##STR00024## ##STR00025## ##STR00026##

(122) Mesylate derivatives having the structure of Formula XVII are also embodied by the present invention.

(123) ##STR00027##

(124) wherein R.sup.11 and R.sup.12 are as defined for Formula I; and

(125) at least one of R.sup.y is OMs and the remaining R.sup.y are a hydrogen, cyano, OMs, CH.sub.2OMs, or CH.sub.2 group.