METHOD OF PRESERVING CUT ROSES DURING TRANSPORTATION AND STORAGE AND SHIPMENT KIT CONTAINING CUT ROSES

Abstract

The present invention provides a method of preserving cut roses in the absence of a water supply. The method comprises: (a) cutting roses having a flower bud or a bloomed flower; and (b) packaging the cut roses in a container without immersing or inserting the stems in a hydration system. Instead, the cut roses are treated before or during the packaging by applying a preservation composition onto the flower buds or bloomed flowers The preservation composition contains at least 5 ppm of plant hormone selected from cytokinin, gibberellins and combinations thereof. A flower shipment kit is also disclosed.

Claims

1. -16. (canceled)

17. A flower shipment kit, comprising: (a) a container defining an inner chamber; and (b) one or more cut roses contained within the inner chamber; wherein flower buds or bloomed flowers of the one or more cut roses contain added plant hormone, selected from the group consisting of cytokinins, gibberellins and combinations thereof; and wherein stems of the cut roses are not immersed or inserted into a hydration system.

18. The flower shipment kit according to claim 17, wherein the buds or flowers of the one or more cut roses contain at least 0.5 ppm of the plant hormone.

19. The flower shipment kit according to claim 17, wherein the plant hormone is a cytokinin.

20. The flower shipment kit according to claim 19, wherein the cytokinin is an adenine-type cytokinin.

21. The flower shipment kit according to claim 20, wherein the adenine-type cytokinin is 6-benzyladenine.

22. The flower shipment kit according to claim 19, wherein the cytokinin is thidiazuron.

23. The flower shipment kit according to claim 17, wherein the container is a box.

24. The flower shipment kit according to claim 17, wherein the added plant hormone at flower buds or bloomed flowers is at a concentration at least twice that of the concentration of the added plant hormone in the stems of the one or more cut roses.

25. The flower shipment kit according to claim 17, wherein the chamber of the container holds at least 10 cut roses.

26. A method of preserving cut roses during transportation or storage, comprising: (a) packaging one or more cut roses having a stem and a flower bud or a bloomed flower in a container without immersing or inserting the stems in a hydration system; (b) applying a preservation composition, before or during the packaging, onto the flower buds or bloomed flowers of the cut roses, wherein the preservation composition comprises at least 5 ppm of plant hormone selected from cytokinin, gibberellins and combinations thereof.

27. The method according to claim 26, wherein the roses are in bud stage when the preservation composition is applied.

28. The method according to claim 26, wherein the preservation composition is applied onto the flower buds or bloomed flowers after the roses have been cut.

29. The method according to claim 26, wherein the preservation composition is applied by spraying or by dipping.

30. The method according to claim 26, further comprising keeping the treated roses in the packaging for at least 1 day under refrigeration conditions and without water supply.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0034] The present invention provides a method of preserving cut roses during transportation or storage, said method comprising: [0035] cutting roses to produce one or more cut roses comprising a flower bud or a bloomed flower; and [0036] packaging one or more of the cut roses in a container without immersing or inserting the stems in a hydration system;
wherein the cut roses are treated before or during the packaging by applying a preservation composition onto the flower buds or bloomed flowers of these cut roses, said preservation composition containing at least 5 ppm of plant hormone selected from cytokinin, gibberellins and combinations thereof.

[0037] The term container as used herein should not be construed narrowly. This term encompasses, for instance, a carton box containing one or more bunches of roses. The term further encompasses, for instance, a shipping container that is filled with numerous bunches of roses.

[0038] The term cytokinin as used herein refers to a class of plant growth substances that promote cell division, or cytokinesis, in plant roots and shoots. There are two types of cytokinins: adenine-type cytokinins represented by kinetin, zeatin, and 6-benzylaminopurine, and phenylurea-type cytokinins like diphenylurea and thidiazuron.

[0039] The term gibberellins as used herein refers to plant hormones that regulate growth and influence various developmental processes. Gibberellins are tetracyclic diterpene acids.

[0040] There are two classes based on the presence of either 19 or 20 carbons. The 19-carbon gibberellins, such as gibberellic acid, have lost carbon 20 and, in place, possess a five-member member lactone bridge that links carbons 4 and 10. The 19-carbon forms are, in general, the biologically active forms of gibberellins.

[0041] Whenever reference is made herein to the cutting of roses, unless indicated otherwise, what is meant is the harvesting of roses by removing one or more roses from a rose shrub by cutting the stem.

[0042] It should be understood that the present method may comprise additional flower treatment steps before the packaging of the cut roses in the container. Prior to said packaging, the stems of the cut roses may suitably be immersed into an aqueous treatment solution containing, for instance, preservatives and/or nutrients. Different scenarios of the present method that include one or more of such pre-treatments (immersion of the stems of the cut roses in a treatment solution) are listed below: [0043] a) cutting, pretreatment, packaging [0044] b) cutting, bunching, pre-treatment, packaging [0045] c) cutting, pre-treatment, bunching, packaging [0046] d) cutting, pre-treatment (greenhouse solution), bunching, pre-treatment (pre-treatment solution), packaging.

[0047] In these scenarios the application of the preservation composition onto the flower buds or bloomed flowers of the cut roses may occur at any stage after the cutting.

[0048] The preservation composition employed in the present method preferably is a liquid, most preferably an aqueous liquid.

[0049] The concentration of plant hormone in the preservation composition preferably lies in the range of 8-200 ppm, more preferably in the range of 10-100 ppm and most preferably in the range of 20-80 ppm.

[0050] The preservation composition may suitably contain other components besides the plant hormone. Examples of other components that may be present in the composition include anti-fungal compounds like boscalid, imazalil, pyrimethanil, cyprodinil, fludioxinil, prochloraz, fenhexamid, tebuconazole and/or thiabendazole.

[0051] The preservation composition is preferably applied onto the flower buds or bloomed flowers by spraying or dipping. Application by dipping can be achieved by immersing at least a part of the cut flower, including the flower bud or bloomed flower, into a liquid preservation composition containing the plant hormone(s).

[0052] According to a particularly preferred embodiment, the preservation composition is applied by dipping the flower buds or bloomed flowers of the cut roses into a bath filled with the liquid preservation composition, preferably whilst holding the stems above the surface of the preservation composition. Typically, the flower buds or bloomed flowers are kept in the liquid preservation composition for 1-60 seconds, more preferably for 2-20 seconds and most preferably for 3-5 seconds.

[0053] Preferably the preservation composition is applied selectively to the flower buds and the bloomed flower, i.e. the leaves and stems are not (purposely) treated.

[0054] In accordance with another preferred embodiment, the treatment with a preservation composition does not include immersing the cut end of the flower stem into the preservation composition for more than 30 minutes, even more preferably for more than 10 minutes.

[0055] The roses preferably are still in the bud stage when they are treated with the preservation composition.

[0056] The time period between the cutting and the packaging of the cut roses may vary widely. If the freshly cut roses are stored under refrigerated dark conditions, packaging may be postponed by a couple of weeks. Typically, the duration of this time period between cutting and packaging does not exceed 14 days. More preferably, the cut roses are packaged within 7 days, more preferably within 1 day after the cutting.

[0057] The preservation composition is advantageously applied onto the flower buds or bloomed flowers at some stage between the cutting of the roses and the packaging of the cut roses. The preservation composition may be applied shortly after the cutting, or alternatively, it may be applied shortly before the packaging. In accordance with a preferred embodiment, the preservation composition is applied within 14 days, more preferably within 7 days and most preferably within 1 day after the cutting.

[0058] The benefits of the present invention are particularly pronounced if, following packaging, the treated roses are kept in the container for at least 1 day without water supply. Even more preferably the treated roses are kept in the container without water supply for at least 2 days, most preferably for at least 4 days.

[0059] In accordance with a particularly preferred embodiment of the present invention the packaged, treated roses are kept in the container for at least 1 day under refrigeration conditions and without water supply. Even more preferably the treated roses are kept in the container under refrigeration conditions without water supply for at least 2 days, most preferably for at least 4 days. Here the term refrigeration conditions refers to cooled transportation or storage at a temperature in the range of 0-10 C., preferably of 0-6 C., most preferably of 0-2 C.

[0060] Another aspect of the present invention relates to a flower shipment kit comprising: [0061] an outer container defining an inner chamber; and [0062] one or more cut roses contained within said inner chamber;
wherein the flower buds or bloomed flowers of the one or more cut roses contain added plant hormone, said added plant hormone being selected from cytokinins, gibberellins and combinations thereof; and wherein the stems of the cut roses are not immersed or inserted into a hydration system.

[0063] The buds or flowers of the one or more cut roses typically contain at least 0.5 ppm of the plant hormone. More preferably the plant hormone (cytokinins and/or gibberellins) is contained in the buds or flowers in a concentration of at least 1 ppm, even more preferably of 2-40 ppm and most preferably of 4-20 ppm.

[0064] The plant hormone employed in accordance with the present invention preferably is a cytokinin. According to one preferred embodiment, the cytokinin is an adenine-type cytokinin, most preferably 6-benzyladenine. According to another preferred embodiment the cytokinin is thidiazuron.

[0065] As explained herein before, the term container should not be construed narrowly. The inner chamber of the container typically has a volume in the range of 10 dm.sup.3 to 1,000 m.sup.3, more preferably of 20 dm.sup.3 to 1 m.sup.3 and most preferably of 40 dm.sup.3 to 200 dm.sup.3.

[0066] Preferably, the container holding the one or more cut roses is a box, more preferably a carton box.

[0067] The number of cut roses within the inner chamber can range of 1 to 500,000. Preferably, the inner chamber of the outer container holds at least 10 cut roses, even more preferably 100-400 cut roses.

[0068] In accordance with the present invention the plant hormone is preferably selectively applied onto the flower buds or bloomed flowers, meaning that more plant hormone is applied onto the buds or flowers than on, for instance, the stems. Accordingly, in a preferred embodiment, the concentration of the added plant hormone in the flower buds or bloomed flowers of the one or more cut roses is at least twice as high, more preferably at least 4 times as high and most preferably at least 5 times as high as the concentration of the added plant hormone in the stems of the one or more cut roses.

[0069] Yet another aspect of the invention relates to the use of a plant hormone selected from cytokinin, gibberellins and combinations thereof for preserving the colour of cut roses (delaying colour fading of cut roses), said use comprising selectively applying the plant hormone onto the flower buds or the bloomed flowers of cut roses.

[0070] This particular use of plant hormone is particularly advantageous in case the cut roses are intensely coloured, e.g red roses, purple roses, orange roses or yellow roses.

[0071] In accordance with this particular use, the plant hormone is preferably applied onto the flower buds or the bloomed flowers within 14 days, more preferably within 7 days and most preferably within 1 day after the harvesting (cutting) of the roses.

[0072] The plant hormone employed to achieve colour preservation preferably is a cytokinin, more preferably an adenine-type cytokinin. According to a particularly preferred embodiment, the plant hormone is 6-benzyladenine.

[0073] The invention is further illustrated by the following non-limiting examples.

EXAMPLES

Example 1

[0074] Freshly cut red roses (Red Desire') were obtained from a greenhouse in the Netherlands. These flowers were randomly divided into three different sets .

[0075] Within 4 hours of cutting, one set of flowers (set A) was subjected to a preservation treatment that involved dipping of the flower heads into an aqueous solution of 80 ppm 6-benzylaminopurine (BA) in tap water for 3 seconds.

[0076] Immediately after the preservation treatment, each set of flowers was bunched and sleeved (6 flowers per sleeved bunch) after the ends of the flower stems had been cut off, and in total 108 flowers of each set were kept 24 hours at 2 C. under dark conditions in buckets (54 stems per bucket) with the flower stems immersed in 2 liters of water containing 1 ml/l of a flower pre-treatment product (Chrysal Grow 20).

[0077] Following this pre-treatment, the sleeved flower bunches of set A were removed from the buckets and packaged without any external water supply in a carton box (lbh=1.00.40.2 m). The carton box contained 18 sleeved flower bunches.

[0078] One of the other two sets of flowers (Control) was packaged in the same way. The remaining set of flowers (Reference) was not packaged dry into a carton box, but immediately subjected to the sequence of storage and display conditions described in Table 1.

TABLE-US-00001 TABLE 1 Temperature Light conditions Duration Refrigerated 2 C. Dark 4 days storage .sup.1 Shop display .sup.1 20 C. Dark/1,000 lux .sup.3 2 days Home display .sup.2 20 C. Dark/1,000 lux .sup.3 Up to 14 days .sup.1 9 sleeved bunches in a bucket holding 2 liter of water; water containing 5 ml/l of Chrysal Clear Professional 2. .sup.2 6 flowers in a vase (without sleeve) holding 1 liter of water; vase water containing 10 g/l of Chrysal Clear. .sup.3 During shop and home display the flowers were alternately exposed to 12 hours of light (1,000 lux) and 12 hours of darkness.

[0079] Before the refrigerated storage and the home display the ends of the flower stems were cut off.

[0080] The packaged flowers (sleeved and in a carton box) belonging to Set A and the Control set were subjected to simulated refrigerated transport under dry, dark conditions for 14 days at 2 C. before also being subjected to the sequence of storage and display conditions described in Table 1.

[0081] Before the refrigerated storage stage the BA concentrations in the flower heads were analyzed. In addition, the vase life of the flowers was assessed by an expert panel during the home display period. The results are depicted in Table 2.

TABLE-US-00002 TABLE 2 BA concentration Vase BA Dry in flower life added transport heads (ppm) (days) Set A Yes Yes 10.0 12.8 Control No Yes 0 11.0 Reference No No 0 12.0

[0082] After 11 days on the vase, the red roses of set A were found to have retained most of their original red colour. In contrast thereto, the roses of the control set showed undesirable bluing/darkening of the colour

Example 2

[0083] Example 1 was repeated, except that this time, instead of using cut roses obtained from a Dutch green house, cut roses that had been air transported under dry conditions from Kenya to the Netherlands were used. Three different types of cut red roses were used in this study, namely Hot Blood, Rhodos and Incredible.

[0084] The results for each of these rose types are summarized in Tables 3, 4 and 5.

TABLE-US-00003 TABLE 3 BA concentration Vase Hot BA Dry in flower life Blood added transport heads (ppm) (days) Set A Yes Yes 7.2 13.9 Control No Yes 0 10.2 Reference No No 0 16.0

[0085] After 11 days on the vase, the red roses of set A were found to have retained most of their original red colour. In contrast thereto, the roses of the control set showed undesirable blueing/darkening of the colour.

TABLE-US-00004 TABLE 4 BA Dry or concentration Vase BA wet in flower life Rhodos added transport heads (ppm) (days) Set A Yes Dry 14.0 14.9 Control No Dry 0 11.0 Reference No Wet 0 11.0

[0086] After 11 days on the vase, the red roses of set A were found to have retained most of their original red colour. In contrast thereto, the roses of the control set showed undesirable blueing/darkening of the colour.

TABLE-US-00005 TABLE 5 BA Dry or concentration Vase BA wet in flower life Incredible added transport heads (ppm) (days) Set A Yes Dry 7.9 13.0 Control No Dry 0 10.1 Reference No Wet 0 8.0

[0087] After 11 days on the vase, the red roses of set A were found to have retained most of their original red colour. In contrast thereto, the roses of the control set showed undesirable blueing/darkening of the colour.

Example 3

[0088] Example 1 was repeated, except that this time no Reference set was used, i.e. the study used two sets of flowers that were both transported without water supply (dry). Furthermore, the cut roses used in the study had been air transported under dry conditions from Colombia to the Netherlands. Bunches containing 4 flowers of each of three different types of roses (Red Intuition (red), Citran (yellow), Vendela (white) were used in this study. The duration of simulated transportation was reduced from 14 to 9 days and the duration of shop display was increased from 2 to 3 days.

[0089] Besides the vase life of the flowers the expert panel also scored visual leaf quality after 12 days on the vase (1=poor, 5=good). The BA concentration in the flower heads was not analyzed in this study.

[0090] The results are shown in Table 6.

TABLE-US-00006 TABLE 6 BA Vase life Leaf added (days) quality Set A Yes 3.0 Red Intuition 9.6 Citran 12.5 Vendela 13.0 Control No 1.0 Red Intuition 5.0 Citran 13.0 Vendela 12.5

Example 4

[0091] Example 3 was repeated, except that this time the following three different types of roses were used in the study: [0092] Freedom (red) [0093] Bonanza (peach) [0094] Sandra (pink)

[0095] The results are shown in Table 7.

TABLE-US-00007 TABLE 7 BA Vase life Leaf added (days) quality Set A Yes 4.0 Freedom 18.8 Bonanza 14.5 Sandra 17.6 Control No 3.0 Freedom 15.3 Bonanza 15.8 Sandra 14.0

Example 5

[0096] Procedure

[0097] Three different types of cut red roses were used in this study, namely Hot Blood, Rhodos and Incredible. For each type flowers were randomly divided into four different sets.

[0098] One set of flowers (set A) was subjected to a preservation treatment that involved dipping of the flower heads into an aqueous solution of 80 ppm 6-benzylaminopurine (BA) in tap water for 3 seconds.

[0099] Immediately after the preservation treatment of set A, each set of flowers was bunched and sleeved (10 flowers per sleeved bunch) after the ends of the flower stems had been cut off, and in total 20 flowers of each set were kept for 4 hours at 20 C. under dark conditions in buckets (60 stems per bucket) with the flower stems immersed in 2 liters of water containing 1 ml/l of a flower pre-treatment product (Chrysal Grow 20).

[0100] Following this pre-treatment, the sleeved flower bunches of set A were removed from the buckets and packaged without any external water supply in a carton box (lbh=1.00.40.2 m). The carton box contained 6 sleeved flower bunches.

[0101] One of the other three sets of non-dipped flowers(Control) was packaged in the same way, i.e. in carton boxes without external water supply.

[0102] The remaining two sets of non-dipped flowers (sets B and C) were introduced into buckets (20 stems per bucket) that each contained 2 liters of water.

[0103] The bucket water of set B contained 7 ppm BA. The bucket water of set C contained 7 ppm BA as well as 5 ml/l of Chrysal Clear Professional 2 (CCP2). This product is based on sugars and acidifier, while biocides are present to protect these active ingredients against bacterial attack.

[0104] The four sets of roses were kept at 2 C. under dark conditions for 24 hours, following which each set was transferred into buckets (60 stems per bucket) that contained 2 liters of water to which 5 ml/l Chrysal Clear Professional 2 had been added. Before introducing the cut flowers into the buckets, the ends of the flower stems were cut off. The buckets holding the sleeved cut flowers were kept for 4 days under dark, refrigerated (2 C.) conditions, and subsequently for 2 days at 20 C. under daylight conditions (12 hours 1000 lux and 12 hours dark).

[0105] Next, for each of the three different type of cut red roses the two bunches of 10 cut flowers from each of the sets were introduced in vases, each holding 1 liter of water to which 10 g/l Chrysal Clear had previously been added. Before introducing the cut flowers into the vases, the ends of the flower stems were cut off. The vases holding the flowers were kept at 20 C. under daylight conditions (12 hours 1000 lux and 12 hours dark).

[0106] The quality of the cut flowers was assessed at regular intervals by an expert panel and from these assessments the average vase life was determined for the cut flowers of each of the 4 sets.

[0107] The results obtained from these experiments are summarized in Table 8

TABLE-US-00008 TABLE 8 Vase life (days) Hot Blood Control 10.7 a A (dipped in BA, dry) 16.2 b B (wet + BA) 11.2 a C (wet + BA + CCP2) 12.0 a Rhodos Control 8.8 a A (dipped in BA, dry) 13.9 b B (wet + BA) 9.4 a C (wet + BA + CCP2) 8.7 a Incredible Control 11.4 a A (dipped in BA, dry) 17.2 b B (wet + BA) 11.1 a C (wet + BA + CCP2) 11.8 a

[0108] The letters behind the numerical scores in Table 8 indicate to which extent the difference between the 4 scores for a given set of cut flowers are statistically significant (at a P-value of 0.05). Scores that are followed by the same latter are not statistically significant.

[0109] Table 8 shows that for each of the rose types the dipped set had a significantly longer vase life than the non-dipped sets.

Example 6

[0110] Example 5 was repeated, except that this time the sets of cut flowers were kept under refrigerated and dark conditions for 8 days instead of 24 hours.

[0111] The results of these experiments are shown in Table 9

TABLE-US-00009 TABLE 9 Vase life (days) Hot Blood Control 9.9 ab A (dipped in BA, dry) 11.8 b B (wet + BA) 7.3 a C (wet + BA + CCP2) 8.9 ab Rhodos Control 9.9 a A (dipped in BA, dry) 13.5 b B (wet + BA) 8.2 a C (wet + BA + CCP2) 8.5 a Incredible Control 12.1 a A (dipped in BA, dry) 14.8 b B (wet + BA) 10.4 a C (wet + BA + CCP2) 11.2 a

[0112] Table 9 shows that for two of the rose types the dipped set had a significantly longer vase life than the non-dipped sets. For rose type Hot Blood the average vase life of the dipped set was higher than that of the non-dipped sets, but the difference with the control set and set C was not statistically significant at a P-value of 0.05.