Method for production of modified cells expressing HOX and modified cells obtained by the method

Abstract

A cell modified for obtaining increased proliferative capacity, decreased aging and enhanced regenerative capacity, its modification involving HOXB7 overexpression obtained using a gene vector that can insert the coding sequence into the cell, thereby affording increased protein production.

Claims

1. A method of obtaining over-expression of a HOXB7 protein encoded by a nucleotide sequence having SEQ ID NO 2 within mesenchymal stem cells (MSCs) to form skeletal tissue, comprising: yielding retroviral particles carrying a coding sequence for said HOXB7 protein; and genetically modifying said MSCs by infecting said MSCs with said retroviral particles to produce modified cells having a faster expansion, a lower senescence rate, and an increased ability to form said skeletal tissue than the MSCs before infecting wherein said MSCs comprise isolated MSCs of human origin chosen from bone marrow, fat, umbilical cord, menstrual liquids, amniotic liquid, synovial liquid, tooth pulp, hepatic tissue, pulmonary tissue, or pancreatic tissue.

2. The method as claimed in claim 1, further comprising a step of inducing said genetically modified MSCs to express higher levels than the MSCs before infecting of one or more of alkaline phosphatase molecular markers (ALP), alpha-1 type I collagen (Col1A1), or decorin (DCN).

3. The method as claimed in claim 2, wherein inducing said genetically MSCs to express higher levels than the MSCs before infecting comprises inducing said genetically modified MSCs with a conditioned medium containing dexamethasone, ascorbic acid, beta-glycerophosphate, and bone morphogenetic protein 2 (BMP2).

4. The method as claimed in claim 2, wherein the step of inducing said genetically modified MSCs to express higher levels than the MSCs before infecting of one or more of alkaline phosphatase molecular markers (ALP), alpha-1 type I collagen (Col1A1), or decorin (DCN) comprises a step of inducing said genetically modified MSCs to form the skeletal tissue with the lower senescence rate.

5. The method as claimed in claim 1, wherein said genetically modified MSCs have an increased production or an increased release of growth factors than unmodified MSCs, and wherein at least one of said growth factors is bFGF.

6. The method as claimed in claim 1, further comprising: collecting a retroviral supernatant from embryonic renal fibroblast cells; infecting a Producer Cell Line of a human fibrosarcoma line; and analyzing after 24 hours by cytofluorimetry a positivity percentage of green fluorescent protein, wherein infecting said MSCs with said retroviral particles comprises infecting said MSCs with retroviral supernatant produced by said Producer Cell Line.

Description

BRIEF DESCRIPTION OF FIGURES

(1) Further features and advantages of the invention will be more readily apparent upon reading of the detailed description of preferred non-exclusive embodiments of a cell modified for HOXB7 overexpression, which is shown as a non-limiting example in the annexed drawings, in which:

(2) FIG. 1 is a schematic representation of the construct that was used for the genetic modification of cells;

(3) FIG. 2 is a chart showing an efficiency analysis of cell transduction using the construct by assessment of the amount of fluorescence emitted by the green fluorescent protein (GFP) coded by the vector in use;

(4) FIG. 3 is a chart showing the analysis of HOXB7 expression in modified cells, assessed both as messenger (mRNA) production by real time PCT and as a protein by Western Blotting;

(5) FIG. 4 shows microscopy images showing primary cells modified for higher HOXB7 expression (image a) and corresponding GFP controls (image b), in which the arrows that indicate the individual cells highlight a smaller cell size in image (a) than in image (b);

(6) FIG. 5 is a chart showing the cytofluorimetric analysis of the physical parameters FSC and SSC, indicating cell size and internal complexity respectively;

(7) FIG. 6 is a chart showing the cyrofluorimetric analysis of characterizing surface antigens that characterize the cells being used and in which the modification has been made;

(8) FIG. 7 is a chart that shows the analysis of Ki67 mRNA expression by real time PCR;

(9) FIG. 8 is a chart for in vitro cell proliferation and growth assessment of modified cells by microscopic cell counting;

(10) FIG. 9 is a chart for the assessment of clonogenic efficiency, i.e. in vitro colony-forming ability of cells;

(11) FIG. 10 shows microscopy images of beta galactosidase-stained cells, identifying senescent cells, in which HOXB7-modified cells exhibit less senescence than GFP control cells;

(12) FIG. 11 is a chart for quantification of beta galactosidase positive cells, i.e. senescent cells, in the cultures of the modified cells;

(13) FIG. 12 is a chart showing the expression of the growth factor bFGF mRNA produced by the modified cells;

(14) FIG. 13 is a chart for ELISA assessment of the amount of bFGF protein released by the modified cells;

(15) FIG. 14 is a microscopy image of Von Kossa staining;

(16) FIG. 15 are charts showing the quantification of stained deposits in the modified cells;

(17) FIGS. 16, 17 and 18 are charts that shows the expression of alkaline phosphatase bone tissue markers (ALP), collagene 1 (Col1A1) and decorin (DCN), in which the black color indicates the controls, i.e. the cells modified with an empty vector, which express GFP but not HOXB7 and the gray color indicates the cells modified for higher HOXB7 expression;

(18) FIG. 19 is a diagrammatic representation of the 3′ UTR (3′ untranslated region) sequence of the HOXB7 gene (SEQ ID NO 1), allowing microRNA-mediated protein expression control; the 3′ UTR sequence of HOXB7 is paired to the microRNA 196 (miR-196) sequence, which prevents HOXB7 protein production (see panel).

(19) FIG. 20 is the diagrammatic representation of the constructs that have been used to show that the expression of HOXB7 gene is regulated in sequence-specific manner by the microRNA miR-196a; the construct expresses luciferase, an enzyme whose action causes a light emission and upstream from the enzyme-coding sequence the HOXB7 3′ UTR region or the deleted 3′ UTR sequence for the above region of interaction with miR-196 (HOXB7 3′ UTR 99-227) has been placed to show the direct interaction therebetween;

(20) FIG. 21 is a chart showing luciferase expression assessment by measurement of the light emission of luciferin upon introduction of miR-196a or control (miR control) into the cells transduced for the constructs as shown in FIG. 20;

(21) FIG. 22 is a chart showing a higher Ki67 expression in the P+ group than in the P− group;

(22) FIG. 23 is a list of the differentially expressed microRNAs by less proliferating cells (P−) and more proliferating cells (P+);

(23) FIG. 24 is a sequence (SEQ ID NO 2) that codes for the HOXB7 protein.

DETAILED DESCRIPTION OF A PREFERRED EMBODIMENT

(24) According to the invention, a HOXB7-coding gene has been introduced, thereby generating a cell that can have greater proliferative potential, less senescence and increases differentiative potential relative to controls having no HOXB7-overexpression modification.

Gene Modification of Cells with HOXB7 Gene

(25) This approach consists in gene modification of cells using gene sequences, known as “vectors” that can induce the expression of proteins, known as HOX.

(26) As shown in FIG. 1, the HOXB7 protein-coding sequence is introduced into the circular plasmid DNA sequence MIGR1 using the insertion sites BgIII and EcoRI.

(27) The plasmid DNA contains the GFP protein-coding sequence which will be used as a label to check that the modification has been made.

(28) HOX genes are transcription factors (i.e. factors that can bind DNA and promote synthesis of other proteins) that can influence proliferation and differentiation of human cells during pre- and postnatal life.

(29) The insertion of HOXB7 protein-producing vectors affords the production of modified cells having increased growth, while facilitating their differentiation toward bone.

Creation of the Construct

(30) The HOXB7 coding gene was produced and amplified by polymerase chain reaction (PCR) from total RNA extracted from hematopoietic cells and corresponds to the sequence filed in PubMed database with the code NM_004502.

(31) The sequence so obtained was inserted into a retroviral vector by enzyme digestion and cloning, as shown in FIG. 1.

(32) A population of carrier cells that can stably produce a pool of retroviral particles capable of infecting the cell population of interest was created through two steps.

(33) The first step is based on the obtainment of a cell line producing the retrovirus in transient mode and the second step is aimed at obtaining the generation of a Producer Cell Line (PCL) capable of stably producing a retroviral progeny.

(34) For the transient step, embryonic renal fibroblasts (293T cells) were transfected with plasmid DNA with the help of polycations to produce a supernatant containing the retroviral particles.

(35) The retroviral supernatant was collected and used to infect the PLC deriving from a human fibrosarcoma line: 24 hours after infection the cells were analyzed by cytofluorimetry to check positivity of green fluorescent protein (GFP), an infection efficiency marker.

(36) The viral supernatant that was in turns stably produced by the PLCs was used to infect the cells of interest.

Transformation of HOXB7-Overexpressing Cells

(37) The cells of interest were isolated from bone marrow aspirations by density gradient centrifugation (Ficoll) and were selected according to their ability of adhering to the plastic medium upon which they are cultivated in vitro.

(38) The cells underwent three infection cycles using the retroviral supernatants produces by the PLCs.

(39) Then infection efficiency was assessed by analysis of the expression of the green fluorescent protein (GFP).

(40) As shown in FIG. 2, this analysis indicates that all the cells were successfully modified (gray bar), similarly to the GFP control (black bar).

Assessment of Overexpression

(41) HOXB7 protein overexpression was verified by quantitative PCR and Western Blotting (FIG. 3).

(42) Quantitative PCR allowed detection of relative expression levels of the mRNA of HOXB7 (>160 fold the control infected with the empty vector, i.e. a vector not containing the HOXB7 coding sequence, while Western Blotting allowed detection of the higher expression of HOXB7, normalized to an endogenous protein constitutively expressed by the cells of interest (GAPDH).

Impact of Overexpression on Morphology and Antigen Expression

(43) The morphology of the modified cells was assessed by inverted microscope observation and by cytofluorimetry (see FIG. 4 and FIG. 5).

(44) HOXB7-overexpressing cells are morphologically different from the control, and particularly have a smaller size and a lower degree of internal complexity, as shown in FIG. 4 by arrows and in FIG. 5, which shows the values of FSC and SSC.

(45) The FSC value reflects cell size and SSC values reflect internal complexity, and both are lower for HOXB7-modified cells (gray bar) than for GFP control cells (black bar).

(46) Therefore, the cells modified for HOXB7 overexpression are smaller and less complex cells.

(47) The cells modified for HOXB7 overexpression have a particular in vitro growth, with a cord arrangement (see FIG. 4(a)).

(48) The surface markers typically expressed by the cell, CD90, CD73 and CD105 are maintained as shown in FIG. 6, with the chart showing that these markers undergo no relevant change due to the modification.

Assessment of Proliferation, Clonogenicity and Senescence

(49) HOXB7-modified cells acquire a higher proliferative potential, which is assessed as an expression of the cell proliferation marker Ki67.

(50) Ki67 is a cell proliferation marker, and its quantity reflects cell growth.

(51) As shown in FIG. 7, the cells modified for HOXB7 overexpression have higher levels of Ki67.

(52) The greater proliferation was also assessed by microscopic cell counting during in vitro expansion.

(53) FIG. 8 shows the higher proliferative capacity of HOXB7-modified cells as compared with GFP controls.

(54) Furthermore, the cells modified for HOXB7 overexpression have a greater colony formation efficiency (CFU-F) as compared with GFP control (see FIG. 9).

(55) Senescence monitoring by beta glactosidase staining allowed detection of a significantly smaller number of senescent cells in HOXB7-modified cultures.

(56) FIG. 10 shows the smaller number of senescent cells (beta-galactosidase positive cells, i.e. stained and indicated by arrows) among modified cells (a) relative to control cells (b).

(57) Referring to FIG. 11, the quantification of stained, senescent cells shows that the GFP control cell population contains a greater number of senescent cells than the cell population modified for HOXB7 overexpression.

Detection of FGF Levels in HOXB7-Overexpressing Cells

(58) HOXB7-overexpressing cells produce higher measured bFGF levels, a growth factor also known as FGF2, both as mRNA expression by real time PCR, as shown in FIG. 12, and as a secreted protein as measured by ELISA, whose values are shown in the chart of FIG. 13.

(59) In greater detail, secretion of the bFGF protein was found to be 4.3 to 8.2 fold higher in HOXB7-modified cells than in the GFP control.

Assessment of Osteogenic Differentiation

(60) The modified cells were induced to form bone tissue using a conditioned medium containing dexamethasone, ascorbic acid, beta-glycerophosphate and BMP2 (the latter being added at day seven), and were found to have a higher bone-forming ability relative to the control.

(61) Particularly, the modified cells produce a significantly greater amount of mineralized matrix, as assessed by Von Kossa staining in vitro.

(62) Von Kossa is a dye that is known to identify calcium deposits released from osteogenically differentiating cells.

(63) As shown in FIG. 14, the cells modified for HOXB7 overexpression (a) are more stained than the GFP control (b).

(64) Furthermore, particularly referring to FIG. 15, Von Kossa staining quantification shows that HOXB7-overexpressing cells (gray bar) produce 8.5 to 12.2 fold increased levels of calcium deposits as compared with GFP control cells (black bar) after 2 weeks' induction, or maintenance of cells in an appropriate bone production-stimulating culture medium.

(65) The modified cells show a higher expression of alkaline phosphatase molecular markers (ALP), alpha-1 type I collagen (Col1A1) and decorin (DCN), as shown in FIG. 16, FIG. 17 and FIG. 18.

(66) In greater detail, the change in the expression of these markers is found to be 2.7 to 41 fold higher in HOXB7-modified cells than in GFP controls.

Identification of Expression Control Mechanisms

(67) MicroRNAs are small RNAs that regulate gene expression by specific recognition of 3′ untranslated regions (3′ UTR) of messengers (mRNA).

(68) Using predictive algorithms we identified miR-196a as a potential molecule that can regulate HOXB7 expression (FIG. 19).

(69) A luciferase assay was used to show that miR-196 directly targets the 3′ UTR region of HOXB7 in cells, and hence can specifically control its expression (FIG. 20 and FIG. 21).

(70) Referring to FIG. 21, the presence of miR-196 is found to reduce light emission (column two) due to the specific interaction of miR-196 with the 3′ UTR sequence of HOXB7.

Identification of Differentially Expressed MicroRNAs of Less Proliferating Cells and More Proliferating Cells, as a Prediction of Cell Performance

(71) A set of microRNAs was identified, with microRNAs differentially expressed by cells with different proliferative potentials (P− and P+).

(72) Referring to FIG. 22 the P− group expresses lower levels of Ki67, thereby exhibiting a lower proliferative potential, and the P+ group expresses higher levels of Ki67, thereby exhibiting a higher proliferative potential.

(73) These two groups with different proliferative potentials show differences in mRNA expression: miR-99, miR-100, miR-196, miR-337-5p, miR-376, miR-431, miR-543 as shown in FIG. 23. This microRNA set may be used to obtain information about cell proliferation capacity.

(74) The invention has been found to fulfill the intended objects.

(75) The invention so conceived is susceptible to changes and variants within the inventive concept.

(76) Furthermore, all the details may be replaced by other technically equivalent elements, without departure from the scope as defined by the following claims.