Bifunctional angiogenesis inhibitor and use thereof

Abstract

The present invention relates to a bifunctional angiogenesis inhibitor, which has VEGF inhibitory activity and FGF inhibitory activity, and can inhibit VEGF and FGF dual factors-induced or high glucose-induced cell proliferation, cell migration, and/or lumen formation. The present invention also relates to the use of the bifunctional angiogenesis inhibitor in inhibiting retinal angiogenesis, such as diabetic retinopathy, age-related macular degeneration and the like.

Claims

1. A bifunctional angiogenesis inhibitor having the amino acid sequence shown in SEQ ID NO: 1.

2. A pharmaceutical composition comprising the polypeptide as claimed in claim 1.

3. A kit comprising the pharmaceutical composition of claim 2.

4. A method of inhibiting VEGF and FGF dual factor-induced cell proliferation, cell migration, and/or lumen formation comprising administering the bifunctional angiogenesis inhibitor of claim 1 to a subject in need thereof.

5. A method of inhibiting retinal angiogenesis comprising administering the bifunctional angiogenesis inhibitor of claim 1 to a subject in need thereof.

6. A method of treating angiogenesis-related eye diseases comprising administering the bifunctional angiogenesis inhibitor of claim 1 to a subject in need thereof.

7. The method according to claim 6, wherein the angiogenesis-related eye diseases are selected from the group consisting of: age-related macular degeneration, diabetic retinopathy (DR), proliferative DR, diabetic macular edema, retinopathy of prematurity and retinal vascular occlusion.

8. A method of improving retinal damage in a subject with diabetes comprising administering the bifunctional angiogenesis inhibitor of claim 1 to the subject.

9. The method according to claim 8, wherein the retinal damage is short-term retinal damage or long-term retinal damage.

10. The method according to claim 9, wherein the improvement to the short-term retinal damage is selected from the group consisting of reducing the number of apoptotic cells in the retinal vascular network, reducing leakage of the blood-retinal barrier, inhibiting reactive proliferation of retinal glial cells, and improving ultrastructure of neural retina and retinal blood vessels; and the improvement to the long-term retinal damage is selected from the group consisting of improving retinal barrier leakage and inhibiting the thickening of capillary basement membrane.

11. A method of improving the avascular perfusion area of the retina or reducing the number of nuclei of retinal neovascularization cells in a subject with retinopathy comprising administering the bifunctional angiogenesis inhibitor of claim 1 to the subject.

12. The method according to claim 11, wherein the subject with retinopathy is a premature infant.

13. An isolated polynucleotide comprising a nucleotide sequence encoding a bifunctional angiogenesis inhibitor, wherein the amino acid sequence of the bifunctional angiogenesis inhibitor is shown in SEQ ID NO: 1.

14. The polynucleotide of claim 13 having the nucleotide sequence shown in SEQ ID NO:3.

15. A nucleic acid construct comprising the polynucleotide of claim 13, wherein the polynucleotide is operably linked to one or more regulatory sequences directing the production of the polypeptide in an expression host.

16. A vector comprising the polynucleotide of claim 13, wherein the polynucleotide is operably linked to one or more regulatory sequences.

17. The vector of claim 16, wherein the vector is an expression vector, and the regulatory sequence directs the production of the polypeptide in an expression host.

18. A host cell comprising the polynucleotide of claim 13, wherein the polynucleotide is operably linked to one or more regulatory sequences directing the production of the polypeptide.

19. The host cell of claim 18, which is a mammalian cell or a humanized cell.

20. A method for preparing a bifunctional angiogenesis inhibitor having the amino acid sequence shown in SEQ ID NO:1 comprising culturing the host cell as claimed in claim 18 under a condition allowing the expression of the bifunctional angiogenesis inhibitor and recovering the inhibitor.

21. The host cell of claim 18, wherein the mammalian cell is a Chinese Hamster Ovary (CHO) cell.

Description

BRIEF DESCRIPTION OF DRAWINGS

(1) FIG. 1. Inhibitory effects of VF28 and RC28-05 on the proliferation of HUVECs cells stimulated by VEGF165.

(2) FIG. 2. Inhibitory effects of VF28 and RC28-05 on the proliferation of HUVECs cells stimulated by bFGF.

DETAILED DESCRIPTION

(3) Definitions:

(4) Unless otherwise defined, all technical and scientific terms used herein have their ordinary meanings as understood by those of ordinary skill in the art. For definitions and terms in the art, those skilled in the art can specifically refer to Current Protocols in Molecular Biology (Ausubel). The abbreviations for amino acid residues are the standard 3-letter and/or 1-letter codes used in the art to refer to one of the 20 commonly used L-amino acids.

(5) Although the numerical ranges and parameter approximations shown in the broad scope of the present invention, the numerical values shown in the specific examples are recorded as accurately as possible. However, any numerical value must inherently comprise a certain error, which is caused by the standard deviation in their respective measurements. In addition, all ranges disclosed herein should be understood as covering any and all subranges subsumed therein. For example, a recorded range of “1 to 10” should be considered to include any and all subranges between the minimum value 1 and the maximum value 10 (including the end points); that is, all subranges starting with the minimum value 1 or greater, such as 1 to 6.1, and subranges ending with a maximum value of 10 or less, such as 5.5 to 10. In addition, any reference referred to as “incorporated herein” should be understood as being incorporated in its entirety.

(6) As used herein, the term “soluble” protein refers to a protein that is soluble in an aqueous solution at the biologically relevant temperature, pH level and osmotic pressure. In some specific technical solutions, the fusion protein of the present invention is a soluble fusion protein.

(7) As used herein, the term “isolated” refers to the following substances and/or entities, which are (1) separated from at least some of the components that were originally associated therewith (in the natural environment and/or in a test setting) and/or (2) produced, prepared and/or manufactured artificially. The separated substances and/or entities can be separated from at least about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, about 95%, about 98%, about 99%, substantially 100%, or 100% of other components that were originally associated therewith. In some specific technical solutions, the fusion protein of the present invention is an isolated fusion protein.

(8) The term “VEGF” as used herein refers to the vascular endothelial growth factor. The term “VEGFR” as used herein refers to vascular endothelial growth factor receptor, which can be VEGFR1, VEGFR2, and/or VEGFR3. Preferably, the VEGFR in the present invention is VEGFR1 and/or VEGFR2, preferably human VEGFR.

(9) The term “FGF” as used herein refers to fibroblast growth factor. The term “FGFR” as used herein refers to fibroblast growth factor receptor, which can be FGFR1, FGFR2, FGFR3, and/or FGFR4. Preferably, the FGFR in the present invention is FGFR1, more preferably human FGFR1.

(10) The term “subject” as used herein includes mammals such as humans, such as domestic animals (such as dogs, cats and the like), domestic animals (such as cows, sheep, pigs, horses and the like) or experimental animals (such as monkeys, rats, mice, rabbits, guinea pigs and the like).

(11) The fusion protein of the present invention can further comprise post-translational modifications. Such modifications include but are not limited to acetylation, carboxylation, glycosylation, phosphorylation, lipidation and acylation. As a result, the modified protein can comprise non-amino acid components, such as polyethylene glycol, lipids, polysaccharides or monosaccharides, and phosphoric acid. The effect of such non-amino acid components on protein function can be tested as described herein. When proteins are produced in cells, post-translational processing can also be important for correct folding and/or fusion protein function. Different cells (such as CHO, HeLa, MDCK, 293, WI38, NIH-3T3, or HEK293) have specific cell machinery and unique mechanisms for these post-translational activities, and different cells can be selected to ensure the correct modification and processing of proteins.

(12) The proteins described herein can be produced by any method known in the art. For example, it can be produced by chemical synthesis or from nucleic acid expression. The peptides used in the present invention can be easily prepared according to well-known standard liquid or preferably solid phase peptide synthesis methods known in the art (see, for example, J. M. Stewart and J. D. Young, Solid Phase Peptide Synthesis, 2nd edition, Pierce Chemical Company, Rockford, Ill. (1984), in M. Bodanzsky and A. Bodanzsky, The Practice of Peptide Synthesis, Springer Verlag, New York (1984)). The fusion protein can be produced using techniques known in the art to form one or more intramolecular crosslinks between cysteine residues located within the polypeptide sequence expected to be included in the protein (see, for example, U.S. Pat. No. 5,478,925). In addition, the fusion protein described herein can be conventionally modified by adding cysteines or biotins to the C-terminus or N-terminus of the fusion protein.

(13) The term “therapeutically effective amount” or “effective amount” as used herein refers to a dose sufficient to demonstrate its benefit to the subject to be administered. The actual amount, as well as the rate and time course of administration will depend on the subject's own condition and severity. The prescription of treatment (for example, the determination of dosage and the like) is ultimately the responsibility of general practitioners and other doctors and depends on them to make decisions, usually considering the disease to be treated, the individual patient's condition, the delivery site, the method of administration, and other factors known for the doctors.

(14) As used herein, the term “VF28” means a specific VEGFR-FGFR fusion protein, which comprises the second Ig-like domain of VEGFR1, the third Ig-like domain of VEGFR2, and a part derived from the intermediate functional sequence region of the FGFR Ig-like domain, the second Ig-like domain of FGFR, the third Ig-like domain of FGFR and an Fc fragment. VF28 is the abbreviation of 28.sup.# fusion protein in Chinese patent 201110131029.X, and the construction process and other information thereof can be found in the disclosure of Chinese patent 201110131029.X. Specifically, the amino acid sequence of VF28 is shown in SEQ ID NO:2.

(15) As used herein, the term “stock solution” refers to a fusion-protein solution that is purified and distributed in an intermediate storage container. The stock solution in the present invention is obtained by affinity chromatography, virus-removal treatment, crude filtration and chromatography as well as precise filtration of the cell culture solution. The term “finished product” as used herein refers to a fusion-protein solution obtained through sterilizing and filtrating the stock solution then packing it in a sterile final container and packaging. The finished product in the present patent is obtained by sterilizing and filtering the stock solution with a certain ratio of auxiliary materials (sodium dihydrogen phosphate, sodium chloride, sucrose, polysorbate 80) added.

(16) As used herein, the term “FGF-Trap” refers to the FGFR-Fc fusion protein, which can be used as a trap for FGF, thereby antagonizing FGF. Specifically, the FGF-Trap used in the examples of the present invention is the 26 #FGFR fusion protein, and the construction process and other information thereof can be found in CN102219860A.

(17) The term “VEGF-Trap” as used herein refers to the VEGFR-Fc fusion protein, which can be used as a trap for VEGF, thereby antagonizing VEGF. Specifically, the VEGF-Trap used in the examples of the present invention is an anti-VEGF recombinant protein (ETLEA, Elia) developed by Regeneron Pharmaceutical Company of the United States, which is commercially available.

(18) The examples are used to further elaborate and explain the present invention, and should not be considered as a limitation of the present invention.

Example 1: Selection of Host Cells

(19) Chinese Hamster Ovary Cells (CHO/dhfr-), which are deficient in dihydrofolate reductase (DHFR) gene, were purchased from ATCC, USA under catalog number CRL-9096 and lot number 3916620. CHO/dhfr-cells were cultured in IMDM complete medium supplemented with hypoxanthine and thymidine (HT) and 10% fetal bovine serum (FBS). The cells were polygonal and growed adherently. After 3 passages, the cells were frozen in liquid nitrogen for storage. In order to obtain CHO/dhfr-cells adapted to serum-free suspension culture, a tube of frozen cells were thawed in a 37° C. water bath, and suspended in Ex-Cell 302 CHO medium (Sigma) containing 10% FBS and HT, then growed adherently in a cell culture flask. When the cells grew well, the cells were suspended in 30 mL of Ex-Cell 302 CHO medium containing 5% fetal bovine serum for shake-flask culture. When the cells grew to 1-2×10.sup.6/mL, the cells were transferred to Ex-Cell 302 CHO cell culture medium containing 2.5% fetal bovine serum for shake-flask culture. Adaptation was thus achieved step by step in Ex Cell 302 medium containing 1.5% and 0.5% fetal bovine serum, respectively, by shake-flask culture. Finally, the cells were suspended in serum-free Ex Cell 302 CHO medium for shake-flask culture. When the cells grew well, the cells were collected by centrifugation, suspended in Ex-Cell 302 CHO medium containing 10% DMSO, and frozen in liquid nitrogen for storage, which is the original cell bank of CHO cells domesticated by serum-free culture. The CHO cells domesticated by serum-free culture were round or nearly round under suspension culture conditions.

Example 2: Gene of Interest

(20) RC28-05 fusion protein is a fusion protein with bifunctional angiogenesis inhibitory activity, which is composed of partial amino acid sequences of VEGFR and FGFR fused with a human immunoglobulin Fc fragment, the amino acid sequence of which is shown below (as shown in SEQ ID NO:1):

(21) TABLE-US-00001 GRPFVEMYSE IPEIIHMTEG RELVIPCRVT SPNITVTLKK FPLDTLIPDG KRIIWDSRKG  60 FIISNATYKE IGLLTCEATV NGHLYKTNYL THRQTNTIID VVLSPSHGIE LSVGEKLVLN 120 CTARTELNVG IDFNWEYPSS KHQHKKLVNR DLKTQSGSEM KKFLSTLTID GVTRSDQGLY 180 TCAASSGLMT KKNSTFVRVH EKPVAPYWTS PEKMEKKLHA VPAAKTVKFK CPSSGTPNPT 240 LRWLKNGKEF KPDHRIGGYK VRYATWSIIM DSVVPSDKGN YTCIVENEYG SINHTYQLDV 300 VERSPHRPIL QAGLPANKTV ALGSNVEFMC KVYSDPQPHI QWLKHIEVNG SKIGPDNLPY 360 VQILKTAGVN TTDKEMEVLH LRNVSFEDAG EYTCLAGNSI GLSHHSAWLT VLEADKTHTC 420 PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN 480 AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP 540 QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL 600 YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K 641

(22) The nucleotide sequence of RC28-05 is shown below (1923 bp) (as shown in SEQ ID NO: 3):

(23) TABLE-US-00002 ggtagaccat tcgtagagat gtacagtgaa atccccgaaa ttatacacat gactgaagga   60 agggagctcg tcattccctg ccgggttacg tcacctaaca tcactgttac tttaaaaaag  120 tttccacttg acactttgat ccctgatgga aaacgcataa tctgggacag tagaaagggc  180 ttcatcatat caaatgcaac gtacaaagaa atagggcttc tgacctgtga agcaacagtc  240 aatgggcatt tgtataagac aaactatctc acacatcgac aaaccaatac aatcatagat  300 gtggttctga gtccgtctca tggaattgaa ctatctgttg gagaaaagct tgtcttaaat  360 tgtacagcaa gaactgaact aaatgtgggg attgacttca actgggaata cccttcttcg  420 aagcatcagc ataagaaact tgtaaaccga gacctaaaaa cccagtctgg gagtgagatg  480 aagaaatttt tgagcacctt aactatagat ggtgtaaccc ggagtgacca aggattgtac  540 acctgtgcag catccagtgg gctgatgacc aagaagaaca gcacatttgt cagggtccat  600 gaaaaacccg tagctccata ttggacatcc ccagaaaaga tggaaaagaa attgcatgca  660 gtgccggctg ccaagacagt gaagttcaaa tgcccttcca gtgggacccc aaaccccaca  720 ctgcgctggt tgaaaaatgg caaagaattc aaacctgacc acagaattgg aggctacaag  780 gtccgttatg ccacctggag catcataatg gactctgtgg tgccctctga caagggcaac  840 tacacctgca ttgtggagaa tgagtacggc agcatcaacc acacatacca gctggatgtc  900 gtggagcggt cccctcaccg gcccatcctg caagcagggt tgcccgccaa caaaacagtg  960 gccctgggta gcaacgtgga gttcatgtgt aaggtgtaca gtgacccgca gccgcacatc 1020 cagtggctaa agcacatcga ggtgaatggg agcaagattg gcccagacaa cctgccttat 1080 gtccagatct tgaagactgc tggagttaat accaccgaca aagagatgga ggtgcttcac 1140 ttaagaaatg tctcctttga ggacgcaggg gagtatacgt gcttggcggg taactctatc 1200 ggactctccc atcactctgc atggttgacc gttctggaag ccgacaaaac tcacacatgc 1260 ccaccgtgcc cagcacctga actcctgggg ggaccgtcag tcttcctctt ccccccaaaa 1320 cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt ggtggacgtg 1380 agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga ggtgcataat 1440 gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt cagcgtcctc 1500 accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa 1560 gccctcccag cccccatcga gaaaaccatc tccaaagcca aagggcagcc ccgagaacca 1620 caggtgtaca ccctgccccc atcccgggat gagctgacca agaaccaggt cagcctgacc 1680 tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag caatgggcag 1740 ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc cttcttcctc 1800 tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt ctcatgctcc 1860 gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct gtctccgggt 1920 aaa 1923

(24) A conventional expression vector were inserted after introducing double cleavage sites at both ends of the RC28-05 target gene sequence. CHO cells were transfected by a general method to select an RC28-05 expressing cell strain, and expression of the RC28-05 fusion protein was performed.

Example 3: Affinity Test of the Fusion Protein

(25) VF28 and RC28-05 were tested for the affinity using ForteBio Octet (PALL). PBS (pH 7.4) was added as a balanced solution to each well of column 1 of the detection plate A-E, and the probe was soaked and activated for 10 min; RC28-05 and VF28 were diluted respectively to a concentration of 50 nM with PBS and added to column 2 of rows A-E of the 96-well assay plate with the program set to Loading for 300 s; PBS was added as a balanced solution to each well of column 3 of rows A-E of the detection plate with the program set to Baseline for 180 s; rhVEGF (R&D) and rhFGF (R&D) were diluted respectively to a concentration of 500 nM and 400 nM using PBS as the diluent, and then serially diluted at 1:2 for three gradients (a total of four gradients) to a concentration of 62.5 nM and 50 nM. The serially diluted samples were added to column 4 of rows A-D of the 96-well plate while the dilution was added to column 4 of row E as a negative control, with the program set to Association for 600 s; PBS was added as the dissociation solution to each well in column 5 of rows A-E with the program set to Dissociation for 1800 s; 10 mM glycine (pH 1.5) and PBS were added as the regeneration solution and the neutralization solution in columns 6 and 7 of rows A-E, respectively, with the programs respectively set to Regeneration and Neutralization for 15 s each, repeating for 5 times. A total of 3 cycles were tested. Data was analysed using Data analysis 7.0 software. The equilibrium constant (KD) value was calculated with the background subtracted using the corresponding unbound rhVEGF/rhFGF sensor as a control. The results were shown in Table 1. The results showed that RC28-05 and VF28 had good affinity with rhVEGF and rhFGF.

(26) TABLE-US-00003 TABLE 1 Statistics of affinity comparison results of RC28-05 and VF28 Equilibrium Binding Constant Dissociation Constant Samples Factors Constant KD (M) kon(1/Ms) kdis(1/s) N RC28-05 rhVEGF165 2.70E−10 ± 1.28E−10 2.30E+05 ± 0.64E+05 5.65E−05 ± 0.75E−05 3 VF28 rhVEGF165 2.51E−10 ± 5.65E−11 2.76E+05 ± 7.94E+04 6.68E−05 ± 1.31E−05 3 RC28-05 rhFGF2 3.28E−09 ± 1.60E−09 2.66E+04 ± 1.01E+04 7.96E−05 ± 2.42E−05 3 VF28 rhFGF2 5.24E−09 ± 2.07E−09 1.90E+04 ± 1.74E+03 9.78E−05 ± 3.26E−05 3

Example 4: Cell Activity Experiment of Fusion Protein

(27) HUVEC cells within 10 passages were inoculated to a 96-well plate at 100 μL/well (i.e. 5000 cells/well) with the density adjusted to 5×10.sup.4 cells/mL. After the cells adhered, conditioned medium or VEGF165 or bFGF factor (40 ng/mL) or VEGF165+different concentrations of RC28-05 or VF28 drugs (final concentrations of 0, 0.0125, 0.0625, 0.125, 0.25, 0.5, 1, 5, 25 nM) or bFGF+different concentrations of RC28-05 or VF28 drugs (final concentrations of 0, 0.0156, 0.0625, 0.25, 0.5, 1, 2, 8, 32 nM) were added at 100 μL/well with a final culture volume of 200 μL/well, 3 replicate wells per sample; culture was continuously performed at 37° C. in a 5% CO2 incubator. 72 hours after drug addition, the culture medium in the 96-well plate was spin-dried, and the endothelial cell basic culture medium containing 10% CCK-8 was added to each well at 100 μL/well. Incubation was performed at 37° C. for 4 hours, and OD450 was detected using a microplate reader. The inhibition rate of the corresponding drugs on the proliferation of HUVEC cells promoted by VEGF165/bFGF at each concentration was calculated as inhibition rate of cell proliferation %=(OD factor−OD(factor+drug))/OD factor×100. The IC.sub.50 values of the drugs were calculated by Prism software, and the inhibition rates of the maximum drug concentration were tested adopting measured values. The differences between RC28-05 and VF28 in inhibiting HUVEC cell proliferation under stimulation of VEGF165/bFGF were compared. The inhibition results of VF28 and RC28-05 on the proliferation of HUVEC cells stimulated by VEGF165/bFGF were shown in FIG. 1 and FIG. 2, and the maximum inhibition rate and IC50 values statistics were shown in Table 2. The results showed that RC28-05 and VF28 had significant inhibitory effects on the proliferation of HUVEC cells stimulated by VEGF165/bFGF.

(28) TABLE-US-00004 TABLE 2 Inhibitory effects of RC28-05 and VF28 on the proliferation of HUVEC cells stimulated by VEGF165/bFGF Stimulating Maximum Inhibition Drugs Factors IC.sub.50(nM) Rate (%) VF28 VEGF.sub.165 0.48 82.34 ± 1.83 bFGF 3.20 70.16 ± 2.79 RC28-05 VEGF.sub.165 0.43 80.19 ± 0.27 bFGF 1.50 70.40 ± 1.05

Example 5: Preparation of RC28-05 Stock Solutions and Finished Products

(29) The preparation method of RC28-05 stock solutions and finished products was the same as that of VF28, which specifically was:

(30) 1) After centrifuging the cell culture solution, the supernatant was collected for protein A affinity chromatography;

(31) 2) After all the collected eluate was treated with organic solvents/detergents (S/D), 1% polysorbate 80 (W/V) and 0.3% tributyl phosphate (W/V) were added to the eluate and placed at 20-25° C. for 6h to inactivate lipid-enveloped viruses;

(32) 3) After the above treatment, Sepharose cation exchange chromatography was used to remove related impurities such as polymers and degradation products;

(33) 4) Anion exchange chromatography was performed on all the collected eluates to remove a small amount of aggregates, CHO host cell proteins, host DNA and endotoxins and the like;

(34) 5) The penetrating fluid after the above chromatography was collected and then nano-membrane filtrated to remove non-lipid enveloped viruses;

(35) 6) After ultrafiltration and concentration of the protein solution after the nano-membrane filtration to a certain concentration (10-15 mg/mL), a buffer solution of 10-12 times the volume of the concentrated protein (0.02 mol/L sodium dihydrogen phosphate, 0.015 mol/L sodium chloride, 0.2 mol/L sucrose, PH 6.8) was used for substitution dialysis, then the protein solution was concentrated (40-45 mg/mL). After 0.02% (W/V) polysorbate 80 was added and stirred to dissolve, filtration was performed using 0.45+0.2 μm membrane, and the resultant protein solution was RC28-05 stock solutions with a protein concentration of 40-45 mg/mL.

(36) 7) Preparation of finished products: The preparation method of 1000 tubes of finished products of 40 mg/mL (0.2 ml/tube) was as follows. The concentration of RC28-05 protein in the above protein stock solutions was determined and defined as C mg/mL, and a total of 220 ml of protein solution needed to be prepared considering 10% of the filling loss. Then the total amount of protein required was 220 ml*40 mg/mL*0.2 ml=8.8 g protein, and the protein stock solution volume required was V=8.8/C*1000 (mL). A buffer solution (0.02 mol/L sodium dihydrogen phosphate, 0.015 mol/L sodium chloride, 0.2 mol/L sucrose, 0.02% (W/V) polysorbate 80) was added to a V mL protein stock solution till a volume of 220 mL and mixed homogeneously. Filteration was performed using a filter membrane with a pore size of not more than 0.22 μm for sterilization. RC28-05 finished products were obtained after packaging, rolling aluminum cover and boxing.

Example 6: Stability Test of RC28-05 (Stock Solutions/Finished Products)

(37) 1. Stability Test of RC28-05 Stock Solutions

(38) (1) Purity Analysis of Different Storage Time (−80° C.±10° C.)

(39) {circle around (1)} SEC-HPLC Method

(40) 3 batches of RC28-05 stock solutions (batch numbers: RC28-05-YY20160329, RC28-05-YY20160330, RC28-05-YY20160331) were placed under a long-term test condition of −80° C.±10° C. for 0 hours, 6 months, 9 months and 12 months for purity analysis (SEC-HPLC method). The test results of the SEC purity of the test samples at each monitoring time point showed that the main peaks were all ≥95.0%. See Table 14 for details.

(41) TABLE-US-00005 TABLE 14 Test results of the SEC purity of RC28-05 stock solutions (−80° C. ± 10° C.) (%) Periods SEC purity % Batches T0 6 m 9 m 12 m RC28-05-YY20160329 98.4 98.2 98.5 100.0 RC28-05-YY20160330 98.0 97.9 98.1 99.8 RC28-05-YY20160331 97.7 97.5 97.8 99.2 Note: The evaluation criterion for the SEC purity was the main peak ≥95.0%; “m” stands for “month”.

(42) {circle around (2)} SDS-PAGE Method

(43) 3 batches of RC28-05 stock solutions (batch numbers: RC28-05-YY20160329, RC28-05-YY20160330, RC28-05-YY20160331) were placed under a long-term test condition of −80° C.±10° C. for 0 hours, 6 months, 9 months and 12 months for purity analysis (SDS-PAGE method). The results were shown in Table 15. The data showed that as the storage time increased, the test results of the SDS-PAGE reducing purity of RC28-05 stock solutions were all higher than 90%.

(44) TABLE-US-00006 TABLE 15 Test results of the SDS-PAGE purity of RC28-05 stock solutions (−80° C. ± 10° C.) (%) Period SDS-PAGE reducing purity % Batches T0 6 m 9 m 12 m RC28-05-YY20160329 98.1 98.3 97.7 97.6 RC28-05-YY20160330 98.1 98.1 97.8 97.5 RC28-05-YY20160331 98.8 98.2 97.1 97.8 Note: The evaluationcriterion for the SDS-PAGE purity was non-reducing purity ≥90%; “m” stands for “month”.

(45) (2) Cell Activity Experiment of Stock Solutions at Different Storage Time (−80° C.±10° C.)

(46) 3 batches of RC28-05 stock solutions (batch numbers: RC28-05-YY20160329, RC28-05-YY20160330, RC28-05-YY20160331) were placed under a long-term test condition of −80° C.±10° C. for 12 months for cell activity analysis; the results showed that it was found through cell activity analysis of RC28-05 stock solutions placed at −80° C.±10° C. for 12 months that the relative activity of the cells at each monitoring time point in the test period all met the quality requirements. See Table 16 for details.

(47) TABLE-US-00007 TABLE 16 Test results of the relative cell activity of RC28-05 stock solutions (−80° C. ± 10° C.) (%) Periods Relative Cell activity % Batches T0 6 m 9 m 12 m RC28-05-YY20160329 85.1 92.8 81.8 103.3 RC28-05-YY20160330 93.0 88.1 77.7 95.7 RC28-05-YY20160331 86.7 100.5 92.7 106.0 Note: The evaluation criterion for the relative cell activity was that the relative cell activity should be 70% to 130%; “m” stands for “month”.

(48) (3) Binding Activity Experiment of Stock Solutions at Different Storage Time (−80° C.±10° C.)

(49) 3 batches of RC28-05 stock solutions (batch numbers: RC28-05-YY20160329, RC28-05-YY20160330, RC28-05-YY20160331) were placed under a long-term test condition of −80° C.±10° C. for 12 months for binding activity analysis (ELISA method); the results showed that it was found through the relative binding activity test analysis of RC28-05 stock solutions placed at −80° C.±10° C. for 12 months that the relative activity of the cells at each monitoring time point in the test period all met the quality requirements. See Table 17 for details.

(50) TABLE-US-00008 TABLE 17 Test results of the relative binding activity of RC28-05 stock solutions (−80° C. ± 10° C.) (%) Periods Relative binding activity T0 Batches VEGF FGF 6 m 9 m 12 m RC28-05-YY20160329 87.6 97.4 101.4 114.6 92.9 RC28-05-YY20160330 120.2 109.6 99.6 105.0 105.6 RC28-05-YY20160331 103.0 94.5 101.5 103.6 118.9 Note: The evaluation criterion for the relative binding activity was that the relative binding activity should be 70% to 130%; T0 was of the dual system analysis, while the remaining time points were of the single system analysis; “m” stands for “month”.

(51) 2. RC28-05 Finished Product Stability Test

(52) (1) Purity Analysis at Different Storage Time

(53) {circle around (1)}SEC-HPLC Method

(54) 3 batches of RC28-05 finished products (batch numbers: RC28-05-20160401-1, RC28-05-20160401-2, RC28-05-20160401-3) were placed under a long-term test condition of 5° C.±3° C. for 12 months, under an accelerated test condition of 25° C.±2° C. for 1 month, respectively, for purity analysis (SEC-HPLC method). The experimental results showed that it was found through purity analysis (SEC-HPLC method) of the finished product placed under a long-term test condition of 5° C.±3° C. for 12 months that the test results of the SEC purity of the test samples at each monitoring time point during the experimental period were all about 95%; whereas when the finished product was placed under an accelerated test condition of 25° C.±2° C. for 1 month, the test results of the SEC purity of the test samples at each monitoring time point showed that the main peak were all ≥95.0%. See Table 18 and Table 19 for details.

(55) TABLE-US-00009 TABLE 18 Test results of the SEC purity of RC28-05 finished products (5° C. ± 3° C.) (%) Periods SEC purity Batches T0 3 m 6 m 9 m 12 m RC28-05-20160401-1 98.4 98.4 97.4 96.9 97.9 RC28-05-20160401-2 98.1 98.1 97.0 96.6 97.1 RC28-05-20160401-3 97.7 97.7 96.7 96.4 96.9 Note: The evaluation criterion for the SEC purity was the main peak ≥95.0%; “m” standed for “month”.

(56) TABLE-US-00010 TABLE 19 Test results of the SEC purity of RC28-05 finished products (25° C. ±2° C.) (%) Periods SEC purity Batches 0 d 10 d 20 d 30 d RC28-05-20160401-1 98.4 96.6 96.1 96.6 RC28-05-20160401-2 98.1 96.3 95.8 96.1 RC28-05-20160401-3 97.7 96.2 95.6 95.9 Note: The evaluation criterion for the SEC purity was the main peak ≥95.0%; “m” stands for “month”.

(57) {circle around (2)} SDS-PAGE Method

(58) 3 batches of RC28-05 finished products (batch numbers: RC28-05-20160401-1, RC28-05-20160401-2, RC28-05-20160401-3) were placed under a long-term test condition of 5° C.±3° C. for 12 month, at 25° C.±2° C. for 1 month, respectively, for purity analysis (SDS-PAGE method). The results showed that it was found through purity analysis (SDS-PAGE) of the RC28-05 finished product placed at 5° C.±3° C. for 12 months that the test results of SDC-PAGE reducing purity of the test samples at each monitoring time point in the test period were all greater than 90.0%; through purity (SDS-PAGE) analysis of the RC28-05 finished product placed at 25° C.±2° C. for 6 months, it was found that the reducing purity of SDC-PAGE of the tested samples at each monitoring time point had a downward trend, while the test results were all greater than 90.0%. See Table 20 and Table 21 for details.

(59) TABLE-US-00011 TABLE 20 Test results of the SDS-PAGE purity of RC28-05 finished products (5° C. ± 3° C.) (%) Periods SDS-PAGE reduction Batches 0 h 3 m 6 m 9 m 12 m RC28-05-20160401-1 98.9 97.7 97.0 96.9 95.7 RC28-05-20160401-2 97.9 97.6 96.7 97.7 95.9 RC28-05-20160401-3 98.0 97.6 97.0 96.9 96.3 Note: The evaluation criterion for the SDS-PAGE purity was non-reducing purity ≥90%; “m” stands for “month”.

(60) TABLE-US-00012 TABLE 21 Test results of the SDS-PAGE reducing purity of RC28-05 finished products (25° C. ± 2° C.) (%) Periods SDS-PAGE reduction Batches 0 h 10 d 20 d 30 d RC28-05-20160401-1 98.9 96.3 97.5 97.8 RC28-05-20160401-2 97.9 96.1 97.4 97.6 RC28-05-20160401-3 98.0 96.0 96.8 98.1 Note: The evaluationcriterion for the SDS-PAGE purity was non-reducing purity ≥90%; “d” stands for “day”.

(61) (2) Cell Activity Experiment of Finished Products at Different Storage Time

(62) 3 batches of finished products of RC28-05 (batch numbers: RC28-05-20160401-1, RC28-05-20160401-2, RC28-05-20160401-3) were placed under a long-term test condition of 5° C.±3° C. for 12 months, at 25° C.±2° C. for 1 month, respectively, for cell activity analysis. The results showed that it was found through cell activity analysis of the finished product placed at 5° C.±3° C. for 12 months that the relative cell activity results of the test samples at each monitoring time point during the test period all met the requirements of the evaluation criteria; the test results of the relative cell activity at each monitoring time point during the test period all met the quality requirements through cell activity analysis of the finished product placed at 25° C.±2° C. for 1 month. See Table 22 and Table 23 for details.

(63) TABLE-US-00013 TABLE 22 Test results of the relative cell activity of RC28-05 finished products (5° C. ± 3° C.) (%) Periods Relative cell activity Batches 0 h 3 m 6 m 9 m 12 m RC28-05-20160401-1 120.8 125.1 111.8 85.4 100.2 RC28-05-20160401-2 104.3 125.1 96.1 85.0 103.4 RC28-05-20160401-3 105.2 111.7 100.5 93.7 100.3 Note: The evaluation criterion for the relative cell activity was that the relative cell activity should be 70% to 130%; “m” stands for “month”.

(64) TABLE-US-00014 TABLE 23 Test results of the relative cell activity of RC28-05 finished products (25° C. ± 2° C.) (%) Periods Relative cell activity Batches 0 h 10 d 20 d 30 d RC28-05-20160401-1 120.8 103.4 88.7 110.0 RC28-05-20160401-2 104.3 93.4 90.6 108.4 RC28-05-20160401-3 105.2 94.4 88.0 104.1 Note: The evaluation criterion for the relative cell activity was that the relative cell activity should be 70% to 130%; “d” stands for “day”.

(65) (3) Binding Activity Experiment of Finished Products at Different Storage Time

(66) 3 batches of RC28-05 finished products (batch numbers: RC28-05-20160401-1, RC28-05-20160401-2, RC28-05-20160401-3) were placed under a long-term test condition of 5° C.±3° C. for 12 months, at 25° C.±2° C. for 1 month, respectively, for binding activity analysis. The results showed that it was found through the binding activity analysis of the finished products placed at 5° C.±3° C. for 12 months that the results of the relative binding activity of the test samples at each monitoring time point during the test period all met the requirements of the evaluation criteria; through the binding activity analysis of the finished products at 25° C.±2° C. for 1 month, the relative binding activity (the VEGF end and the FGF end) test results at each monitoring time point in the test period all met the quality requirements. See Table 24 and Table 25 for details.

(67) TABLE-US-00015 TABLE 24 Test results of the relative binding activity of RC28-05 finished products (5° C. ± 3° C.) (%) Periods Relative binding activity Batches 0 h (VEGF; FGF) 3 m 6 m 9 m 12 m RC28-05-20160401-1 72.8; 90.5 111.6 107.6 104.9 119.3 RC28-05-20160401-2 76.9; 76.5 116.1 102.4 112.0 109.8 RC28-05-20160401-3 85.5; 86.1 107.1 98.5 110.2 111.1 Note: The evaluation criterion for the relative binding activity was that the relative binding activity should be 70% to 130%; 0 h was of the dual system analysis, while the remaining time points were of the single system analysis; “m” stands for “month”.

(68) TABLE-US-00016 TABLE 25 Test results of the relative binding activity of RC28-05 finished products (25° C. ± 2° C.) (%) Periods Relative binding activity Batches 0 h (VEGF; FGF) 10 d 20 d 30 d RC28-05-20160401-1 72.8; 90.5 112.4 104.5 95.7 RC28-05-20160401-2 76.9; 76.5 109.4 120.5 101.2 RC28-05-20160401-3 85.5; 86.1 107.8 118.6 113.1 Note: The evaluation criterion for the relative binding activity was that the relative binding activity should be 70% to 130%; 0 h was of the dual system analysis, while the remaining time points were of the single system analysis; “d” stands for “day”.

(69) 3. Experimental Conclusions

(70) The storage of 3 batches of RC28-05 stock solutions (batch numbers: RC28-05-YY20160329, RC28-05-YY20160330, RC28-05-YY20160331) under a long-term test condition of −80° C.±10° C. was investigated. The results were:

(71) {circle around (1)} SEC purity: 3 batches of RC28-05 stock solutions were placed at a condition of −80° C.±10° C. for 12 months with their SEC purity all ≥90%.

(72) {circle around (2)} SDS-PAGE reducing purity: 3 batches of RC28-05 stock solutions were placed at −80° C.±10° C. for 12 months with their reducing purity was all ≥90%.

(73) {circle around (3)} Cell activity: 3 batches of RC28-05 stock solutions were placed at −80° C.±10° C. for 12 months. It was found through the cell activity analysis that relative activity of the cells at each monitoring time point in the test period all met the quality requirements.

(74) {circle around (4)} Binding activity: 3 batches of RC28-05 stock solutions were placed at −80° C.±10° C. for 12 months. It was found through the cell activity analysis that relative activity of the cells at each monitoring time point in the test period all met the quality requirements.

(75) The storage of 3 batches of RC28-05 finished products (batch numbers: RC28-05-20160401-1, RC28-05-20160401-2, RC28-05-20160401-3) under a condition of 5° C.±3° C., 25° C.±2° C., respectively, was investigated. The results were:

(76) {circle around (1)} Sec Purity:

(77) 3 batches of RC28-05 finished products were placed at a condition of 5° C.±3° C. for 12 months with their SEC purity all ≥90%;

(78) 3 batches of RC28-05 finished products were placed at a condition of 25° C.±2° C. for 1 month with their SEC purity all ≥90%.

(79) {circle around (2)} SDS-PAGE Reducing Purity:

(80) 3 batches of RC28-05 finished products of RC28-05 were placed at a condition of 5° C.±3° C. for 12 months with their SDS-PAGE reducing purity all ≥90%;

(81) 3 batches of RC28-05 finished products of RC28-05 were placed at a condition of 25° C.±2° C. for 1 months with their SDS-PAGE reducing purity all ≥90%.

(82) {circle around (3)} Cell Activity:

(83) 3 batches of RC28-05 finished products were placed at a condition of 5° C.±3° C. for 12 months, and the test results of cell activity of the test samples at each monitoring time point all met the requirements of the evaluation criteria;

(84) 3 batches of RC28-05 finished products were placed at a condition of 25° C.±2° C. for 1 month, and the test results of cell activity of the test samples at each monitoring time point all met the requirements of the evaluation criteria.

(85) {circle around (4)} Binding Activity:

(86) 3 batches of RC28-05 finished products were placed at 5° C.±3° C. for 12 months, and the test results of cell activity of the test samples at each monitoring time point all met the requirements of the evaluation criteria;

(87) 3 batches of RC28-05 finished products were placed at 25° C.±2° C. for 1 month, and the test results of cell activity of the test samples at each monitoring time point all met the requirements of the evaluation criteria.

(88) From this, the following conclusions can be drawn:

(89) {circle around (1)} RC28-05 stock solutions can be stored stably for at least 12 months under the condition of −80° C.±10° C., whereas VF28 stock solutions did not meet the activity requirements after 6 months under the same condition.

(90) {circle around (2)} RC28-05 finished products can be stored stably for at least 12 months under the condition of 5° C.±3° C., whereas VF28 was stored stably under the same condition for 6 months.

(91) {circle around (3)} RC28-05 finished products can be stored stably at 25° C.±2° C. for at least 1 month, whereas ⅓ of the batches of VF28 stock solution did not meet the activity requirements under the same condition.

(92) In summary, RC28-05 stock solutions were stable at −80° C.±10° C., while RC28-05 finished products were stored stably under the condition of 5° C.±3° C. (for at least 12 months) and 25° C.±2° C. (for at least 1 month), the stable storage time of which was much longer than VF28 under the same condition.

(93) The present invention has been exemplified by various specific examples. However, a person of ordinary skill in the art can understand that the present invention is not limited to each specific embodiments, and a person of ordinary skill can make various changes or modifications within the scope of the present invention, and each technical feature mentioned in various places in this specification can be combined with each other without departing from the spirit and scope of the present invention. Such changes and modifications are within the scope of the present invention.