Method of diagnosis of the aesthetic qualities of the skin

Abstract

The present invention relates to a method for diagnosing a degradation of esthetic qualities of the skin, based on a measurement of the level of expression of the gene encoding FLG2. The present invention also relates to a method for the cosmetic treatment of skin. The present invention also relates to a method for identifying a compound for reducing and/or slowing the degradation of esthetic quality of the skin, and a kit.

Claims

1. A method for cosmetic treatment of a skin for which the esthetic qualities are degraded in a subject, wherein the degraded esthetic qualities are selected from the reduction in shine, appearance of a rough texture, or an increase in skin flaccidity in a subject, wherein the subject does not suffer from dermatological lesions or a dermatological pathology, said method comprising the following steps: a) measuring the level of expression of the gene encoding FLG2 in a skin sample from said subject; wherein the level of expression of the gene encoding FLG2 is determined by measuring the level of expression of the protein encoded by the gene; b1) determining that the level of expression of the gene encoding FLG2 measured in step a) is statistically lower than a reference value, wherein the reference value is about 166 ng/ml of FLG2; and c1) treating the skin of the subject with a statistically lower expression level than the reference value with a cosmetic composition suitable for treating rough texture of the skin of the subject; or b2) determining that the level of expression of the gene encoding FLG2 measured in step a) is statistically higher than a reference value, wherein the reference value is about 140 ng/ml of FLG2 for shine and wherein the reference value is about 82 ng/ml of FLG2 for the flaccidity; and c2) treating the skin of the subject with a statistically higher expression level than the reference value with a cosmetic composition suitable for treating a reduction in the shine and/or an increase in the flaccidity of the skin of the subject.

2. The method according to claim 1, in which the skin sample is taken using an adhesive surface.

Description

EXAMPLE

(1) The reduction in the shine, roughness and flaccidity were evaluated on the cheeks of 376 women aged 36 to 75 years. The level of protein expression of the gene encoding FLG2 was also evaluated on these women's cheeks, by means of a D-Squame sample wherein the soluble protein extract was analyzed using an ELISA test conducted on a chip.

(2) The univariate statistical analysis of the results obtained shows a correlation between the degradation of esthetic qualities of the skin and the expression of FLG2. In particular, beyond the threshold values defined for flaccidity and for shine in Table 1, the skin is likely to evolve towards a flaccid skin or lose its shine, respectively. Below the threshold values defined for roughness in Table 1, the skin is likely to evolve towards a rough skin.

(3) TABLE-US-00001 TABLE 1 Threshold value (in Clinical sign Accuracy Sensitivity Specificity ng/ml) p-values Shine 66% 77% 53% 166 <0.001 Roughness 63% 72% 53% 144 <0.001 Flaccidity 58% 64% 51% 97