ANTIVIRAL ANIMAL FEED ADDITIVE

Abstract

An antiviral animal feed additive is an enzyme and is made from the following ingredients: Artemisia argyi, Lactuca sativa, Allium tuberosum, Allium cepiforme, Litsea rubescensLeeomte, Glycyrrhiza uralensis, Allium sativum, Mentha canadensis, Allium mongolicum, Lonicera japonica, Sargassum, monazite, calcium carbonate, fructo-oligosaccharide (FOS), isomaltooligosaccharide (IMO), and organic selenium. The antiviral animal feed additive can be used to prevent from influenza A virus H1N1 and its complications, and has been performed effectiveness and safety tests in a virus research institute, indicating that the antiviral animal feed additive does not damage animal cells. Moreover, the enzyme is added into animal feed to achieve a good antiviral effect.

Claims

1. An antiviral animal feed additive, which is an enzyme; and wherein the enzyme is made from the following ingredients: 300 grams (g) to 500 g of Artemisia argyi, 200 g to 300 g of Lactuca sativa, 200 g to 300 g of Allium tuberosum, 300 g to 400 g of Allium cepiforme, 200 g to 300 g of Litsea rubescensLeeomte, 50 g to 150 g of Glycyrrhiza uralensis, 50 g to 150 g of Allium sativum, 75 g to 150 g of Mentha canadensis, 100 g to 200 g of Allium mongolicum, 50 g to 150 g of Lonicera japonica, 80 g to 120 g of Sargassum, 20 g to 30 g of monazite, 25 g to 35 g of calcium carbonate, 10 g to 20 g of fructo-oligosaccharide (FOS), 8 g to 12 g of isomaltooligosaccharide (IMO), and 20 g to 40 g of organic selenium.

2. The antiviral animal feed additive as claimed in claim 1, wherein the enzyme is a toxin-dissolving biomimetic enzyme.

3. The antiviral animal feed additive as claimed in claim 2, wherein a weight percentage of the toxin-dissolving biomimetic enzyme in animal feed is in a range of 0.2%-1.0%.

4. The antiviral animal feed additive as claimed in claim 2, wherein a weight percentage of the toxin-dissolving biomimetic enzyme in animal feed is in a range of 0.4%-0.8%.

5. The antiviral animal feed additive as claimed in claim 2, wherein a weight percentage of the toxin-dissolving biomimetic enzyme in animal feed is 0.5%.

6. The antiviral animal feed additive as claimed in claim 1, wherein the enzyme is made from the following ingredients: 300 g of the Artemisia argyi, 200 g of the Lactuca sativa, 200 g of the Allium tuberosum, 300 g of the Allium cepiforme, 200 g of the Litsea rubescensLeeomte, 50 g of the Glycyrrhiza uralensis, 50 g of the Allium sativum, 75 g of the Mentha canadensis, 100 g of the Allium mongolicum, 50 g of the Lonicera japonica, 80 g of the Sargassum, 20 g of the monazite, 25 g of the calcium carbonate, 10 g of the FOS, 8 g of the IMO, and 20 g of the organic selenium.

7. The antiviral animal feed additive as claimed in claim 1, wherein the enzyme is made from the following ingredients: 500 g of the Artemisia argyi, 300 g of the Lactuca sativa, 300 g of the Allium tuberosum, 400 g of the Allium cepiforme, 300 g of the Litsea rubescensLeeomte, 150 g of the Glycyrrhiza uralensis, 100 g of the Allium sativum, 150 g of the Mentha canadensis, 200 g of the Allium mongolicum, 150 g of the Lonicera japonica, 120 g of the Sargassum, 30 g of the monazite, 35 g of the calcium carbonate, 20 g of the FOS, 12 g of the IMO, and 40 g of the organic selenium.

8. The antiviral animal feed additive as claimed in claim 1, wherein the enzyme is prepared by the following steps: step 1, drying the Lactuca sativa, the Allium tuberosum, the Litsea rubescensLeeomte, the Glycyrrhiza uralensis, the Allium mongolicum, and the Lonicera japonica individually according to their weight to obtain dried materials, grinding the dried materials in a grinder to obtain grinded materials, decocting the grinded materials by using purified water to obtain decoction, and then filtering the decoction to obtain a slurry; soaking the Artemisia argyi and the Mentha canadensis into water for 1 hour (h) to obtain soaked materials, distilling the soaked materials by a steam distillation method to obtain a distilled product, and then adding the distilled product into water to perform a time of extraction by reducing pressure to obtain an extracted product, filtering and concentrating the extracted product to a target relative density, thereby obtaining a medicinal liquid 1 for later use; and crushing the Allium sativum and the Allium cepiforme to obtain crushed materials, adding the crushed materials into water to perform a warm infusion for 3 h in a hermetic environment to obtain an infused mixture, adding ethanol into the infused mixture until a concentration of the ethanol is 50%, thereafter performing an extraction by reducing pressure to obtain a product, filtering and concentrating the product to a target relative density, thereby obtaining a medicinal liquid 2; step 2, adding the medicinal liquid 1, the medicinal liquid 2, and the slurry into a material mixing tank and then stirring evenly to obtain a mixed liquid; step 3, adding the Sargassum into the mixed liquid obtained in the step 2, and stewing the mixed liquid added with the Sargassum to obtain a stewed product, taking out the stewed product to filter and cool down to obtain a cooled mixed liquid; step 4, adding 20% of the weight of the FOS and 20% of the weight of the IMO into the cooled mixed liquid obtained in the step 3, and then stirring uniformly, and placing the cooled mixed liquid added with the FOS and the IMO into a fermentation tank for a first fermentation to obtain a first fermentation broth; taking out and filtering the first fermentation broth to obtain a filtered first fermentation broth, adding the remained FOS and the remained IMO into the filtered first fermentation broth, and then placing the filtered first fermentation broth added with the remained FOS and the remained IMO into the fermentation tank for a second fermentation to obtain a second fermentation broth; step 5, crushing the monazite and the calcium carbonate to obtain mixed powder; placing the mixed powder into a furnace with a temperature of 2,000-2,200 degrees Celsius ( C.) and then sintering for 75-105 minutes (min) to obtain sintered powder; cooling the sintered powder to obtain cooled powder, placing the cooled powder into a cloth bag with 800-1,200 meshes and then distilling the cooled powder with a steamer to obtain a distilled liquid for later use; and step 6, distilling the second fermentation broth obtained in the step 4 until there is no distillate to obtain a distilled fermentation broth for later use; mixing the distilled fermentation broth, the distilled liquid obtained in the step 5, and the organic selenium completely to obtain a mixed liquid; adding the mixed liquid into olive oil according to a volume ratio of the mixed liquid to the olive oil of 1:5-10, thereby obtaining the enzyme.

9. The antiviral animal feed additive as claimed in claim 3, wherein the animal feed is antiviral.

10. The antiviral animal feed additive as claimed in claim 8, wherein in the step 6, the volume ratio of the mixed liquid to the olive oil is 1:5.

11. The antiviral animal feed additive as claimed in claim 8, wherein in the step 6, the volume ratio of the mixed liquid to the olive oil is 1:10.

12. The antiviral animal feed additive as claimed in claim 1, wherein the organic selenium is one selected from the group consisting of selenium enriched yeast, kappa-selenocarrageenan, selenium enriched Capsella bursa-pastoris powder extract, selenium enriched Cardamine occulta powder extract, selenium enriched Brassica rapa powder extract, selenium enriched Brassica oleracea powder extract, and selenium enriched Fructus Hordei germinatus powder extract.

13. A preparation method of an antiviral animal feed additive, comprising the following steps: step 1, providing 300 g to 500 g of Artemisia argyi, 200 g to 300 g of Lactuca sativa, 200 g to 300 g of Allium tuberosum, 300 g to 400 g of Allium cepiforme, 200 g to 300 g of Litsea rubescensLeeomte, 50 g to 150 g of Glycyrrhiza uralensis, 50 g to 150 g of Allium sativum, 75 g to 150 g of Mentha canadensis, 100 g to 200 g of Allium mongolicum, 50 g to 150 g of Lonicera japonica, 80 g to 120 g of Sargassum, 20 g to 30 g of monazite, 25 g to 35 g of calcium carbonate, 10 g to 20 g of FOS, 8 g to 12 g of IMO, and 20 g to 40 g of organic selenium; step 2, drying the Lactuca sativa, the Allium tuberosum, the Litsea rubescensLeeomte, the Glycyrrhiza uralensis, the Allium mongolicum, and the Lonicera japonica individually to obtain dried materials, grinding the dried materials in a grinder to obtain grinded materials, decocting the grinded materials by using purified water to obtain decoction, and then filtering the decoction to obtain a slurry; soaking the Artemisia argyi and the Mentha canadensis into water for 1 h to obtain soaked materials, distilling the soaked materials by a steam distillation method to obtain a distilled product, and then adding the distilled product into water to perform a time of extraction by reducing pressure to obtain an extracted product, filtering and concentrating the extracted product to a target relative density, thereby obtaining a medicinal liquid 1 for later use; and crushing the Allium sativum and the Allium cepiforme to obtain crushed materials, adding the crushed materials into water to perform a warm infusion for 3 h in a hermetic environment to obtain an infused mixture, adding ethanol into the infused mixture until a concentration of the ethanol is 50%, thereafter performing an extraction by reducing pressure to obtain a product, filtering and concentrating the product to a target relative density, thereby obtaining a medicinal liquid 2; step 3, adding the medicinal liquid 1, the medicinal liquid 2, and the slurry into a material mixing tank and then stirring evenly to obtain a mixed liquid; step 4, adding the Sargassum into the mixed liquid obtained in the step 2, and stewing the mixed liquid added with the Sargassum to obtain a stewed product, taking out the stewed product to filter and cool down to obtain a cooled mixed liquid; step 5, adding 20% of the weight of the FOS and 20% of the weight of the IMO into the cooled mixed liquid obtained in the step 4, and then stirring uniformly, and placing the cooled mixed liquid added with the FOS and the IMO into a fermentation tank for a first fermentation to obtain a first fermentation broth; taking out and filtering the first fermentation broth to obtain a filtered first fermentation broth, adding the remained FOS and the remained IMO into the filtered first fermentation broth, and then placing the filtered first fermentation broth added with the remained FOS and the remained IMO into the fermentation tank for a second fermentation to obtain a second fermentation broth; step 6, crushing the monazite and the calcium carbonate to obtain mixed powder; placing the mixed powder into a furnace with a temperature of 2,000-2,200 C. and then sintering for 75-105 min to obtain sintered powder; cooling the sintered powder to obtain cooled powder, placing the cooled powder into a cloth bag with 800-1,200 meshes and then distilling the cooled powder with a steamer to obtain a distilled liquid for later use; and step 7, distilling the second fermentation broth obtained in the step 5 until there is no distillate to obtain a distilled fermentation broth for later use; mixing the distilled fermentation broth, the distilled liquid obtained in the step 6, and the organic selenium completely to obtain a mixed liquid; adding the mixed liquid into olive oil according to a volume ratio of the mixed liquid to the olive oil of 1:5-10, thereby obtaining the antiviral animal feed additive.

14. The preparation method as claimed in claim 13, wherein the step 1 comprises: providing 300 g of the Artemisia argyi, 200 g of the Lactuca sativa, 200 g of the Allium tuberosum, 300 g of the Allium cepiforme, 200 g of the Litsea rubescensLeeomte, 50 g of the Glycyrrhiza uralensis, 50 g of the Allium sativum, 75 g of the Mentha canadensis, 100 g of the Allium mongolicum, 50 g of the Lonicera japonica, 80 g of the Sargassum, 20 g of the monazite, 25 g of the calcium carbonate, 10 g of the FOS, 8 g of the IMO, and 20 g of the organic selenium.

15. The preparation method as claimed in claim 14, wherein in the step 6, the temperature is 2,000 C., a time of the sintering is 105 min, and the cloth bag is 800 meshes.

16. The preparation method as claimed in claim 13, wherein the step 1 comprises: providing 500 g of the Artemisia argyi, 300 g of the Lactuca sativa, 300 g of the Allium tuberosum, 400 g of the Allium cepiforme, 300 g of the Litsea rubescensLeeomte, 150 g of the Glycyrrhiza uralensis, 100 g of the Allium sativum, 150 g of the Mentha canadensis, 200 g of the Allium mongolicum, 150 g of the Lonicera japonica, 120 g of the Sargassum, 30 g of the monazite, 35 g of the calcium carbonate, 20 g of the FOS, 12 g of the IMO, and 40 g of the organic selenium.

17. The preparation method as claimed in claim 16, wherein in the step 6, the temperature is 2,200 C., a time of the sintering is 75 min, and the cloth bag is 1,200 meshes.

18. The preparation method as claimed in claim 13, wherein the organic selenium is one selected from the group consisting of selenium enriched yeast, kappa-selenocarrageenan, selenium enriched Capsella bursa-pastoris powder extract, selenium enriched Cardamine occulta powder extract, selenium enriched Brassica rapa powder extract, selenium enriched Brassica oleracea powder extract, and selenium enriched Fructus Hordei germinatus powder extract.

19. An application method of the antiviral animal feed additive as claimed in claim 1, comprising: adding the antiviral animal feed additive into animal feed to feed animals; and wherein a weight percentage of the antiviral animal feed additive in the animal feed is in a range of 0.2%-1.0%.

20. A composite, used to prepare an antiviral animal feed additive, wherein the composite comprises the following materials: 300 g to 500 g of Artemisia argyi, 200 g to 300 g of Lactuca sativa, 200 g to 300 g of Allium tuberosum, 300 g to 400 g of Allium cepiforme, 200 g to 300 g of Litsea rubescensLeeomte, 50 g to 150 g of Glycyrrhiza uralensis, 50 g to 150 g of Allium sativum, 75 g to 150 g of Mentha canadensis, 100 g to 200 g of Allium mongolicum, 50 g to 150 g of Lonicera japonica, 80 g to 120 g of Sargassum, 20 g to 30 g of monazite, 25 g to 35 g of calcium carbonate, 10 g to 20 g of FOS, 8 g to 12 g of IMO, and 20 g to 40 g of organic selenium.

Description

DETAILED DESCRIPTION OF EMBODIMENTS

[0030] In order to facilitate a better understanding of the disclosure by those skilled in the related art, the following is a clear and complete description of the technical solution in embodiments of the disclosure. Apparently, the described embodiments are only a part of the embodiments of the disclosure, not all of the embodiments of the disclosure. Based on the embodiments in the disclosure, all of other embodiments obtained by those skilled in the related art without creative labor shall fall within the scope of the protection of the disclosure.

Embodiment 1

[0031] An enzyme for killing viruses (also referred to as an enzyme, i.e., an antiviral animal feed additive) and a preparation method thereof are provided. Specially, the enzyme is made from the following ingredients:

[0032] 300 g of Artemisia argyi, 200 g of Lactuca sativa, 200 g of Allium tuberosum, 300 g of Allium cepiforme, 200 g of Litsea rubescensLeeomte, 50 g of Glycyrrhiza uralensis, 50 g of Allium sativum, 75 g of Mentha canadensis, 100 g of Allium mongolicum, 50 g of Lonicera japonica, 80 g of Sargassum, 20 g of monazite, 25 g of calcium carbonate, 10 g of fructo-oligosaccharide (FOS), 8 g of isomaltooligosaccharide (IMO), and 20 g of organic selenium.

[0033] The preparation method of the enzyme in the embodiment 1 is as follows: [0034] step 1, drying the Lactuca sativa, the Allium tuberosum, the Litsea rubescensLeeomte, the Glycyrrhiza uralensis, the Allium mongolicum, and the Lonicera japonica individually according to their weight to obtain dried materials, grinding the dried materials in a grinder to obtain grinded materials, decocting the grinded materials by using purified water to obtain decoction, and then filtering the decoction to obtain a slurry; soaking the Artemisia argyi and the Mentha canadensis into water for 1 hour (h) to obtain soaked materials, distilling the soaked materials by a steam distillation method to obtain a distilled product, and then adding the distilled product into water to perform a time of extraction by reducing pressure to obtain an extracted product, filtering and concentrating the extracted product to a target relative density, thereby obtaining a medicinal liquid 1 for later use; and crushing the Allium sativum and the Allium cepiforme to obtain crushed materials, adding the crushed materials into water to perform a warm infusion for 3 h in a hermetic environment to obtain an infused mixture, adding ethanol into the infused mixture until a concentration of the ethanol is 50%, thereafter performing an extraction by reducing pressure to obtain a product, filtering and concentrating the product to a target relative density, thereby obtaining a medicinal liquid 2; [0035] step 2, adding the medicinal liquid 1, the medicinal liquid 2, and the slurry into a material mixing tank and then stirring evenly to obtain a mixed liquid; [0036] step 3, adding the Sargassum into the mixed liquid obtained in the step 2, and stewing the mixed liquid added with the Sargassum to obtain a stewed product, taking out the stewed product to filter and cool down to obtain a cooled mixed liquid; [0037] step 4, adding 20% of the weight of the FOS and 20% of the weight of the IMO into the cooled mixed liquid obtained in the step 3, and then stirring uniformly, and placing the cooled mixed liquid added with the FOS and the IMO into a fermentation tank for a first fermentation to obtain a first fermentation broth; taking out and filtering the first fermentation broth to obtain a filtered first fermentation broth, adding the remained FOS and the remained IMO into the filtered first fermentation broth, and then placing the filtered first fermentation broth added with the remained FOS and the remained IMO into the fermentation tank for a second fermentation to obtain a second fermentation broth; [0038] step 5, crushing the monazite and the calcium carbonate to obtain mixed powder; placing the mixed powder into a furnace with a temperature of 2,000 degrees Celsius ( C.), and then sintering for 105 minutes (min) to obtain sintered powder; cooling the sintered powder to obtain cooled powder, placing the cooled powder into a cloth bag with 800 meshes and then distilling the cooled powder with a steamer to obtain a distilled liquid for later use; and [0039] step 6, distilling the second fermentation broth obtained in the step 4 until there is no distillate to obtain a distilled fermentation broth for later use; mixing the distilled fermentation broth, the distilled liquid obtained in the step 5, and the organic selenium completely to obtain a mixed liquid; adding the mixed liquid into olive oil according to a volume ratio of the mixed liquid to the olive oil of 1:5, thereby obtaining the enzyme.

Embodiment 2

[0040] An enzyme for killing viruses and a preparation method thereof are provided. Specially, the enzyme is made from the following ingredients: [0041] 500 g of Artemisia argyi, 300 g of Lactuca sativa, 300 g of Allium tuberosum, 400 g of Allium cepiforme, 300 g of Litsea rubescensLeeomte, 150 g of Glycyrrhiza uralensis, 100 g of Allium sativum, 150 g of Mentha canadensis, 200 g of Allium mongolicum, 150 g of Lonicera japonica, 120 g of Sargassum, 30 g of monazite, 35 g of calcium carbonate, 20 g of FOS, 12 g of IMO, and 40 g of organic selenium.

[0042] The preparation method of the enzyme in the embodiment 2 is as follows: [0043] step 1, drying the Lactuca sativa, the Allium tuberosum, the Litsea rubescensLeeomte, the Glycyrrhiza uralensis, the Allium mongolicum, and the Lonicera japonica individually according to their weight to obtain dried materials, grinding the dried materials in a grinder to obtain grinded materials, decocting the grinded materials by using purified water to obtain decoction, and then filtering the decoction to obtain a slurry; soaking the Artemisia argyi and the Mentha canadensis into water for 1 h to obtain soaked materials, distilling the soaked materials by a steam distillation method to obtain a distilled product, and then adding the distilled product into water to perform a time of extraction by reducing pressure to obtain an extracted product, filtering and concentrating the extracted product to a target relative density, thereby obtaining a medicinal liquid 1 for later use; and crushing the Allium sativum and the Allium cepiforme to obtain crushed materials, adding the crushed materials into water to perform a warm infusion for 3 h in a hermetic environment to obtain an infused mixture, adding ethanol into the infused mixture until a concentration of the ethanol is 50%, thereafter performing an extraction by reducing pressure to obtain a product, filtering and concentrating the product to a target relative density, thereby obtaining a medicinal liquid 2; [0044] step 2, adding the medicinal liquid 1, the medicinal liquid 2, and the slurry into a material mixing tank and then stirring evenly to obtain a mixed liquid; [0045] step 3, adding the Sargassum into the mixed liquid obtained in the step 2, and stewing the mixed liquid added with the Sargassum to obtain a stewed product, taking out the stewed product to filter and cool down to obtain a cooled mixed liquid; [0046] step 4, adding 20% of the weight of the FOS and 20% of the weight of the IMO into the cooled mixed liquid obtained in the step 3, and then stirring uniformly, and placing the cooled mixed liquid added with the FOS and the IMO into a fermentation tank for a first fermentation to obtain a first fermentation broth; taking out and filtering the first fermentation broth to obtain a filtered first fermentation broth, adding the remained FOS and the remained IMO into the filtered first fermentation broth, and then placing the filtered first fermentation broth added with the remained FOS and the remained IMO into the fermentation tank for a second fermentation to obtain a second fermentation broth; [0047] step 5, crushing the monazite and the calcium carbonate to obtain mixed powder; placing the mixed powder into a furnace with a temperature of 2,200 C. and then sintering for 75 min to obtain sintered powder; cooling the sintered powder to obtain cooled powder, placing the cooled powder into a cloth bag with 1,200 meshes and then distilling the cooled powder with a steamer to obtain a distilled liquid for later use; and [0048] step 6, distilling the second fermentation broth obtained in the step 4 until there is no distillate to obtain a distilled fermentation broth for later use; mixing the distilled fermentation broth, the distilled liquid obtained in the step 5, and the organic selenium completely to obtain a mixed liquid; adding the mixed liquid into olive oil according to a volume ratio of the mixed liquid to the olive oil of 1:10, thereby obtaining the enzyme.

Comparative Example 1

[0049] An enzyme for killing viruses and a preparation method thereof are provided. Specially, the enzyme is made from the following ingredients: [0050] 200 g of Allium tuberosum, 200 g of Litsea rubescensLeeomte, 50 g of Glycyrrhiza uralensis, 50 g of Allium sativum, 75 g of Mentha canadensis, 100 g of Allium mongolicum, 50 g of Lonicera japonica, 80 g of Sargassum, 20 g of monazite, 25 g of calcium carbonate, 10 g of FOS, 8 g of IMO, and 20 g of organic selenium.

[0051] The preparation method of the enzyme in the comparative example 1 is as follows: [0052] step 1, drying the Allium tuberosum, the Litsea rubescensLeeomte, the Glycyrrhiza uralensis, the Allium mongolicum, and the Lonicera japonica individually according to their weight to obtain dried materials, grinding the dried materials in a grinder to obtain grinded materials, decocting the grinded materials by using purified water to obtain decoction, and then filtering the decoction to obtain a slurry; soaking the Mentha canadensis into water for 1 h to obtain a soaked material, distilling the soaked material by a steam distillation method to obtain a distilled product, and then adding the distilled product into water to perform a time of extraction by reducing pressure to obtain an extracted product, filtering and concentrating the extracted product to a target relative density, thereby obtaining a medicinal liquid 1 for later use; and crushing the Allium sativum to obtain a crushed material, adding the crushed material into water to perform a warm infusion for 3 h in a hermetic environment to obtain an infused mixture, adding ethanol into the infused mixture until a concentration of the ethanol is 50%, thereafter performing an extraction by reducing pressure to obtain a product, filtering and concentrating the product to a target relative density, thereby obtaining a medicinal liquid 2; [0053] step 2, adding the medicinal liquid 1, the medicinal liquid 2, and the slurry into a material mixing tank and then stirring evenly to obtain a mixed liquid; [0054] step 3, adding the Sargassum into the mixed liquid obtained in the step 2, and stewing the mixed liquid added with the Sargassum to obtain a stewed product, taking out the stewed product to filter and cool down to obtain a cooled mixed liquid; [0055] step 4, adding 20% of the weight of the FOS and 20% of the weight of the IMO into the cooled mixed liquid obtained in the step 3, and then stirring uniformly, and placing the cooled mixed liquid added with the FOS and the IMO into a fermentation tank for a first fermentation to obtain a first fermentation broth; taking out and filtering the first fermentation broth to obtain a filtered first fermentation broth, adding the remained FOS and the remained IMO into the filtered first fermentation broth, and then placing the filtered first fermentation broth added with the remained FOS and the remained IMO into the fermentation tank for a second fermentation to obtain a second fermentation broth; [0056] step 5, crushing the monazite and the calcium carbonate to obtain mixed powder; placing the mixed powder into a furnace with a temperature of 2,000 C. and then sintering for 105 min to obtain sintered powder; cooling the sintered powder to obtain cooled powder, placing the cooled powder into a cloth bag with 800 meshes and then distilling the cooled powder with a steamer to obtain a distilled liquid for later use; and [0057] step 6, distilling the second fermentation broth obtained in the step 4 until there is no distillate to obtain a distilled fermentation broth for later use; mixing the distilled fermentation broth, the distilled liquid obtained in the step 5, and the organic selenium completely to obtain a mixed liquid; adding the mixed liquid into olive oil according to a volume ratio of the mixed liquid to the olive oil of 1:5, thereby obtaining the enzyme.

Comparative Example 2

[0058] An enzyme for killing viruses and a preparation method thereof are provided. Specially, the enzyme is made from the following ingredients: [0059] 500 g of Artemisia argyi, 300 g of Lactuca sativa, 300 g of Allium tuberosum, 400 g of Allium cepiforme, 300 g of Litsea rubescensLeeomte, 150 g of Glycyrrhiza uralensis, 100 g of Allium sativum, 150 g of Mentha canadensis, 200 g of Allium mongolicum, 150 g of Lonicera japonica, 120 g of Sargassum, 35 g of calcium carbonate, 20 g of FOS, 12 g of IMO, and 40 g of organic selenium.

[0060] The preparation method of the enzyme in the comparative example 2 is as follows: [0061] step 1, drying the Lactuca sativa, the Allium tuberosum, the Litsea rubescensLeeomte, the Glycyrrhiza uralensis, the Allium mongolicum, and the Lonicera japonica individually according to their weight to obtain dried materials, grinding the dried materials in a grinder to obtain grinded materials, decocting the grinded materials by using purified water to obtain decoction, and then filtering the decoction to obtain a slurry; soaking the Artemisia argyi and the Mentha canadensis into water for 1 h to obtain soaked materials, distilling the soaked materials by a steam distillation method to obtain a distilled product, and then adding the distilled product into water to perform a time of extraction by reducing pressure to obtain an extracted product, filtering and concentrating the extracted product to a target relative density, thereby obtaining a medicinal liquid 1 for later use; and crushing the Allium sativum and the Allium cepiforme to obtain crushed materials, adding the crushed materials into water to perform a warm infusion for 3 h in a hermetic environment to obtain an infused mixture, adding ethanol into the infused mixture until a concentration of the ethanol is 50%, thereafter performing an extraction by reducing pressure to obtain a product, filtering and concentrating the product to a target relative density, thereby obtaining a medicinal liquid 2; [0062] step 2, adding the medicinal liquid 1, the medicinal liquid 2, and the slurry into a material mixing tank and then stirring evenly to obtain a mixed liquid; [0063] step 3, adding the Sargassum into the mixed liquid obtained in the step 2, and stewing the mixed liquid added with the Sargassum to obtain a stewed product, taking out the stewed product to filter and cool down to obtain a cooled mixed liquid; [0064] step 4, adding 20% of the weight of the FOS and 20% of the weight of the IMO into the cooled mixed liquid obtained in the step 3, and then stirring uniformly, and placing the cooled mixed liquid added with the FOS and the IMO into a fermentation tank for a first fermentation to obtain a first fermentation broth; taking out and filtering the first fermentation broth to obtain a filtered first fermentation broth, adding the remained FOS and the remained IMO into the filtered first fermentation broth, and then placing the filtered first fermentation broth added with the remained FOS and the remained IMO into the fermentation tank for a second fermentation to obtain a second fermentation broth; [0065] step 5, crushing the calcium carbonate to obtain powder; placing the powder into a furnace with a temperature of 2,200 C. and then sintering for 75 min to obtain sintered powder; cooling the sintered powder to obtain cooled powder, placing the cooled powder into a cloth bag with 1,200 meshes and then distilling the cooled powder with a steamer to obtain a distilled liquid for later use; and [0066] step 6, distilling the second fermentation broth obtained in the step 4 until there is no distillate to obtain a distilled fermentation broth for later use; mixing the distilled fermentation broth, the distilled liquid obtained in the step 5, and the organic selenium completely to obtain a mixed liquid; adding the mixed liquid into olive oil according to a volume ratio of the mixed liquid to the olive oil of 1:10, thereby obtaining the enzyme.

Test Example 1

[0067] The enzymes obtained in the embodiments 1-2 and the comparative examples 1-2 are entrusted to Wuhan Institute of Virology to conduct experiments. The experiments are performed as follows: taking Vero E6 cells with a ratio of 1.510.sup.4 cells/well to inoculate in a 48-well cell culture plate, respectively, and then culturing the 48-well cell culture plate in a 5% CO.sub.2 incubator at 37 C. for 12-16 h; removing supernatant from the cells in the 48-well cell culture plate and incubating the cells for 1 h; adding SARS-coronavirus-2 into the cells at a biosafety level 3 (BSL-3) laboratory to infect the cells with a multiplicity of infection (referred to as a ratio of viruses to cells during infection and abbreviated as MOI) of 0.05; and then incubating the infected cells for 1 h, then removing supernatant thereof, washing the 48-well cell culture plate by using phosphate buffered saline (PBS), and adding the enzymes with different concentrations obtained from the embodiments or the comparative examples, and infecting the cells in the 48-well cell culture plate for 24 h to collect corresponding supernatant of the cells in the 48-well cell culture plate. 150 microliters (L) of the supernatant is charged from each well of the 48-well cell culture plate, and then the supernatant is added into 271 L of lysis solution to inactivate, thereby obtaining inactivated samples loaded in tubes; and then the tubes loaded with the inactivated samples are added with disinfectants, thereafter taking the tubes out of the BSL-3.

[0068] Table 1 illustrates an antibacterial effect of sample solution (i.e., the enzyme) obtained from the embodiment 1 on influenza A virus H1N1.

TABLE-US-00001 Air virus Virus Experiment content inactivation Virus name Action time number (TCID50/m.sup.3) rate/% Influenza A 0 (acting on canine 1 3.24 10.sup.6 virus H1N1 kidney abbreviated 2 2.40 10.sup.6 Host name: as CK) 3 3.24 10.sup.6 Madin-Darby 2 h 1 <1.62 10.sup.2 >99 canine kidney 2 <1.62 10.sup.2 >99 (MDCK) cell 3 <1.62 10.sup.2 >99

[0069] Table 2 illustrates effects of products (i.e., the antiviral animal feed additive) prepared by the disclosure on influenza A virus H1N1.

TABLE-US-00002 Average Logarithmic Average total value of total number virus number of of viruses Concentration titration viruses after 24 h Virus Experimental and time lgTCID50/ lgTCID50/ lgTCID50/ inactivation virus and host of action Group mL mL mL rate/% Influenza Sample Embodiment 1 <1.5 <1.5 <31.6 >99.99 A virus solution 24 h Embodiment 2 <1.5 H1N1 Comparative 4.5 4.55 2.40 10.sup.5 Host name: example 1 MDCK cell Comparative 4.6 example 2

[0070] Experimental conclusion: the products prepared by the disclosure are capable of killing over 99.99% of the influenza A virus H1N1 in the air. After the products are made into the sample solution, the killing rate of influenza A virus H1N1 is greater than 99.99%, indicating that the products obtained from the disclosure indeed have broad-spectrum antiviral effects.

[0071] Apparently, the above-mentioned embodiments are only used to clearly illustrate the disclosure, and are not intended to limit an implementation mode of the disclosure. The objective of the disclosure is to enable those skilled in the related art to understand the content of the disclosure and implement the disclosure according to the content, and the embodiments above cannot limit the scope of the protection of the disclosure. Any modification, equivalent replacement and improvement made within the spirit and principle of the disclosure shall fall within the scope of the protection of the disclosure.